Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57637.t1 | XP_013458487.1 | 81.8 | 247 | 43 | 1 | 1 | 247 | 1 | 245 | 8.40E-37 | 164.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57637.t1 | A0A072UTW6 | 81.8 | 247 | 43 | 1 | 1 | 247 | 1 | 245 | 6.0e-37 | 164.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050207 | MS.gene57637 | 0.803702 | 3.07E-49 | -1.69E-46 |
| MS.gene051867 | MS.gene57637 | 0.921231 | 4.67E-88 | -1.69E-46 |
| MS.gene058208 | MS.gene57637 | 0.807128 | 5.88E-50 | -1.69E-46 |
| MS.gene060038 | MS.gene57637 | 0.806494 | 8.00E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57637.t1 | MTR_4g126210 | 90.837 | 251 | 13 | 3 | 1 | 247 | 1 | 245 | 3.71e-148 | 413 |
| MS.gene57637.t1 | MTR_4g126170 | 38.403 | 263 | 67 | 10 | 1 | 246 | 5 | 189 | 1.10e-30 | 112 |
| MS.gene57637.t1 | MTR_1g016100 | 31.359 | 287 | 144 | 7 | 8 | 245 | 17 | 299 | 2.03e-28 | 110 |
| MS.gene57637.t1 | MTR_2g039320 | 34.263 | 251 | 108 | 9 | 7 | 245 | 12 | 217 | 9.25e-25 | 98.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57637.t1 | AT2G26110 | 36.940 | 268 | 130 | 11 | 6 | 244 | 43 | 300 | 1.83e-29 | 112 |
| MS.gene57637.t1 | AT5G56980 | 32.159 | 227 | 113 | 8 | 50 | 244 | 156 | 373 | 3.55e-18 | 83.2 |
| MS.gene57637.t1 | AT5G56980 | 29.762 | 168 | 93 | 6 | 6 | 148 | 5 | 172 | 3.95e-11 | 62.8 |
| MS.gene57637.t1 | AT4G26130 | 33.798 | 287 | 131 | 12 | 6 | 244 | 5 | 280 | 9.27e-17 | 78.2 |
Find 55 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCTTTACCTCATCGCTGAC+CGG | 0.346958 | 8.3:-4303035 | None:intergenic |
| CTGCAACAAATGATGGTTCT+TGG | 0.349234 | 8.3:-4302752 | None:intergenic |
| ACCATCATTTGTTGCAGAAC+CGG | 0.359413 | 8.3:+4302758 | MS.gene57637:CDS |
| GTTGTAGTGACGGAGGTTAA+AGG | 0.369452 | 8.3:-4302730 | None:intergenic |
| CGCTGACCGGGTTCAGAGAT+GGG | 0.379247 | 8.3:-4303022 | None:intergenic |
| CTCCTCGCCGCCGCCGTCGC+TGG | 0.383420 | 8.3:-4303140 | None:intergenic |
| CTTCACTTCTCAACCGTATC+AGG | 0.388196 | 8.3:+4302706 | MS.gene57637:CDS |
| ATCATTGTAAACCTTGTCAT+AGG | 0.394513 | 8.3:+4302600 | MS.gene57637:CDS |
| GAGGATGTTGTTGTTGGATT+TGG | 0.402134 | 8.3:-4302668 | None:intergenic |
| AAGCTGATGATTTCATCAAC+AGG | 0.418680 | 8.3:+4303204 | MS.gene57637:CDS |
| AAGAAGGCCAGCGACGGCGG+CGG | 0.457267 | 8.3:+4303133 | MS.gene57637:CDS |
| TGAGTTGAGGATGTTGTTGT+TGG | 0.463508 | 8.3:-4302674 | None:intergenic |
| TCGCTGACCGGGTTCAGAGA+TGG | 0.480905 | 8.3:-4303023 | None:intergenic |
| TCCGGTTCTGCAACAAATGA+TGG | 0.484555 | 8.3:-4302759 | None:intergenic |
| CATGTTGCTATGAAAGTTGT+TGG | 0.489388 | 8.3:-4302555 | None:intergenic |
| GTTGATTCAGACTCAGACTC+GGG | 0.495280 | 8.3:-4302906 | None:intergenic |
| TGTTGATTCAGACTCAGACT+CGG | 0.501879 | 8.3:-4302907 | None:intergenic |
| TTCGTGTTTGTTGAGATTGA+AGG | 0.508523 | 8.3:-4302853 | None:intergenic |
| GCTCTAGCTTCTCGCTTCAA+TGG | 0.509488 | 8.3:+4302630 | MS.gene57637:CDS |
| TTCGTGTTTGTTGAGATTGA+AGG | 0.512433 | 8.3:-4302976 | None:intergenic |
| TTCATCTTCTTTGCACGTCG+TGG | 0.520246 | 8.3:-4303168 | None:intergenic |
| GGAGGAAGAAGATGAAGAAA+CGG | 0.520656 | 8.3:+4303103 | MS.gene57637:CDS |
| AAGAAACGGTGGAGAGAAGA+AGG | 0.523159 | 8.3:+4303117 | MS.gene57637:CDS |
| CGAGCTCGAAAACGTTTGAA+AGG | 0.532874 | 8.3:+4303081 | MS.gene57637:CDS |
| TCTTTACCTCATCGCTGACC+GGG | 0.535702 | 8.3:-4303034 | None:intergenic |
| GAGAAGAAGGCCAGCGACGG+CGG | 0.537567 | 8.3:+4303130 | MS.gene57637:CDS |
| GGCCAGCGACGGCGGCGGCG+AGG | 0.541710 | 8.3:+4303138 | MS.gene57637:CDS |
| ATAACTCTCTCTAACAAAGA+AGG | 0.544656 | 8.3:-4302828 | None:intergenic |
| TCGGACTCTGACTCTACCTC+CGG | 0.548761 | 8.3:-4302777 | None:intergenic |
| GAGTTGAGGCTGAGTGAACT+CGG | 0.556334 | 8.3:-4302796 | None:intergenic |
| AGAAACAACTACGGTTGCAA+AGG | 0.557099 | 8.3:+4303231 | MS.gene57637:CDS |
| CTCGAAAACGTTTGAAAGGG+AGG | 0.563627 | 8.3:+4303085 | MS.gene57637:CDS |
| ACAATGATGAAAATAGAAGA+AGG | 0.575111 | 8.3:-4302585 | None:intergenic |
| GGAGAGAAGAAGGCCAGCGA+CGG | 0.586100 | 8.3:+4303127 | MS.gene57637:CDS |
| GGAGGTTAAAGGACCTGATA+CGG | 0.586832 | 8.3:-4302719 | None:intergenic |
| ATGTTGCTATGAAAGTTGTT+GGG | 0.590580 | 8.3:-4302554 | None:intergenic |
| CTGATACGGTTGAGAAGTGA+AGG | 0.593968 | 8.3:-4302705 | None:intergenic |
| GAGCTCGAAAACGTTTGAAA+GGG | 0.597585 | 8.3:+4303082 | MS.gene57637:CDS |
| GGAAGAAGATGAAGAAACGG+TGG | 0.601259 | 8.3:+4303106 | MS.gene57637:CDS |
| AATCTCAACAAACACGAACC+AGG | 0.605009 | 8.3:+4302981 | MS.gene57637:CDS |
| CAACAACTTTCATAGCAACA+TGG | 0.606436 | 8.3:+4302556 | MS.gene57637:CDS |
| TGAAAACCCATCTCTGAACC+CGG | 0.607759 | 8.3:+4303016 | MS.gene57637:CDS |
| ATGGTTCTTGGTTGTAGTGA+CGG | 0.608061 | 8.3:-4302740 | None:intergenic |
| GAAGCGAGAAGCTAGAGCTA+TGG | 0.615154 | 8.3:-4302625 | None:intergenic |
| TAATGTGTCTGTGGATATTG+TGG | 0.622076 | 8.3:-4302876 | None:intergenic |
| ATCATTTGTTGCAGAACCGG+AGG | 0.633321 | 8.3:+4302761 | MS.gene57637:CDS |
| CAGCGATGAGGTAAAGAGCA+AGG | 0.634647 | 8.3:+4303040 | MS.gene57637:CDS |
| GTTCTTGGTTGTAGTGACGG+AGG | 0.636961 | 8.3:-4302737 | None:intergenic |
| ATGACTCGTTGTAACAGAGA+CGG | 0.646476 | 8.3:-4302951 | None:intergenic |
| ACAGGTTTAAGAAACAACTA+CGG | 0.649684 | 8.3:+4303222 | MS.gene57637:CDS |
| TCTGAACCCGGTCAGCGATG+AGG | 0.655711 | 8.3:+4303028 | MS.gene57637:CDS |
| GGTTGAACGTAATGTGTCTG+TGG | 0.666874 | 8.3:-4302885 | None:intergenic |
| GAAGGTGAACGATTGAGTTG+AGG | 0.672108 | 8.3:-4302687 | None:intergenic |
| GAGCTATGGTACCTATGACA+AGG | 0.680413 | 8.3:-4302611 | None:intergenic |
| CGATGAGGTAAAGAGCAAGG+TGG | 0.704311 | 8.3:+4303043 | MS.gene57637:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACAATGATGAAAATAGAAGA+AGG | - | chr8.3:4302588-4302607 | None:intergenic | 25.0% |
| ACAGGTTTAAGAAACAACTA+CGG | + | chr8.3:4303222-4303241 | MS.gene57637:CDS | 30.0% | |
| ATAACTCTCTCTAACAAAGA+AGG | - | chr8.3:4302831-4302850 | None:intergenic | 30.0% | |
| ATCATTGTAAACCTTGTCAT+AGG | + | chr8.3:4302600-4302619 | MS.gene57637:CDS | 30.0% | |
| ATGTTGCTATGAAAGTTGTT+GGG | - | chr8.3:4302557-4302576 | None:intergenic | 30.0% | |
| AAGCTGATGATTTCATCAAC+AGG | + | chr8.3:4303204-4303223 | MS.gene57637:CDS | 35.0% | |
| CAACAACTTTCATAGCAACA+TGG | + | chr8.3:4302556-4302575 | MS.gene57637:CDS | 35.0% | |
| CATGTTGCTATGAAAGTTGT+TGG | - | chr8.3:4302558-4302577 | None:intergenic | 35.0% | |
| TAATGTGTCTGTGGATATTG+TGG | - | chr8.3:4302879-4302898 | None:intergenic | 35.0% | |
| ! | TTCGTGTTTGTTGAGATTGA+AGG | - | chr8.3:4302856-4302875 | None:intergenic | 35.0% |
| !! | TTGTTGGATTTGGTCGTTTT+TGG | - | chr8.3:4302661-4302680 | None:intergenic | 35.0% |
| !!! | TGTTGGATTTGGTCGTTTTT+GGG | - | chr8.3:4302660-4302679 | None:intergenic | 35.0% |
| AATCTCAACAAACACGAACC+AGG | + | chr8.3:4302858-4302877 | MS.gene57637:CDS | 40.0% | |
| AGAAACAACTACGGTTGCAA+AGG | + | chr8.3:4303231-4303250 | MS.gene57637:CDS | 40.0% | |
| ATGACTCGTTGTAACAGAGA+CGG | - | chr8.3:4302954-4302973 | None:intergenic | 40.0% | |
| ATGGTTCTTGGTTGTAGTGA+CGG | - | chr8.3:4302743-4302762 | None:intergenic | 40.0% | |
| CTGCAACAAATGATGGTTCT+TGG | - | chr8.3:4302755-4302774 | None:intergenic | 40.0% | |
| GAGCTCGAAAACGTTTGAAA+GGG | + | chr8.3:4303082-4303101 | MS.gene57637:CDS | 40.0% | |
| GGAGGAAGAAGATGAAGAAA+CGG | + | chr8.3:4303103-4303122 | MS.gene57637:CDS | 40.0% | |
| ! | ACCATCATTTGTTGCAGAAC+CGG | + | chr8.3:4302758-4302777 | MS.gene57637:CDS | 40.0% |
| ! | GAGGATGTTGTTGTTGGATT+TGG | - | chr8.3:4302671-4302690 | None:intergenic | 40.0% |
| ! | TGAGTTGAGGATGTTGTTGT+TGG | - | chr8.3:4302677-4302696 | None:intergenic | 40.0% |
| !! | TGTTGATTCAGACTCAGACT+CGG | - | chr8.3:4302910-4302929 | None:intergenic | 40.0% |
| AAGAAACGGTGGAGAGAAGA+AGG | + | chr8.3:4303117-4303136 | MS.gene57637:CDS | 45.0% | |
| CGAGCTCGAAAACGTTTGAA+AGG | + | chr8.3:4303081-4303100 | MS.gene57637:CDS | 45.0% | |
| CTCGAAAACGTTTGAAAGGG+AGG | + | chr8.3:4303085-4303104 | MS.gene57637:CDS | 45.0% | |
| CTGATACGGTTGAGAAGTGA+AGG | - | chr8.3:4302708-4302727 | None:intergenic | 45.0% | |
| CTTCACTTCTCAACCGTATC+AGG | + | chr8.3:4302706-4302725 | MS.gene57637:CDS | 45.0% | |
| GAGCTATGGTACCTATGACA+AGG | - | chr8.3:4302614-4302633 | None:intergenic | 45.0% | |
| GGAAGAAGATGAAGAAACGG+TGG | + | chr8.3:4303106-4303125 | MS.gene57637:CDS | 45.0% | |
| GGAGGTTAAAGGACCTGATA+CGG | - | chr8.3:4302722-4302741 | None:intergenic | 45.0% | |
| GGTTGAACGTAATGTGTCTG+TGG | - | chr8.3:4302888-4302907 | None:intergenic | 45.0% | |
| TCCGGTTCTGCAACAAATGA+TGG | - | chr8.3:4302762-4302781 | None:intergenic | 45.0% | |
| TGAAAACCCATCTCTGAACC+CGG | + | chr8.3:4303016-4303035 | MS.gene57637:CDS | 45.0% | |
| TTCATCTTCTTTGCACGTCG+TGG | - | chr8.3:4303171-4303190 | None:intergenic | 45.0% | |
| ! | ATCATTTGTTGCAGAACCGG+AGG | + | chr8.3:4302761-4302780 | MS.gene57637:CDS | 45.0% |
| ! | GAAGGTGAACGATTGAGTTG+AGG | - | chr8.3:4302690-4302709 | None:intergenic | 45.0% |
| ! | GAAGGTTTTCGAACGAGTTG+AGG | - | chr8.3:4302813-4302832 | None:intergenic | 45.0% |
| ! | GTTGATTCAGACTCAGACTC+GGG | - | chr8.3:4302909-4302928 | None:intergenic | 45.0% |
| ! | GTTGTAGTGACGGAGGTTAA+AGG | - | chr8.3:4302733-4302752 | None:intergenic | 45.0% |
| !! | AGAGATGGGTTTTCAGCTTC+GGG | - | chr8.3:4303011-4303030 | None:intergenic | 45.0% |
| !! | CAGAGATGGGTTTTCAGCTT+CGG | - | chr8.3:4303012-4303031 | None:intergenic | 45.0% |
| !!! | TGGATTTGGTCGTTTTTGGG+TGG | - | chr8.3:4302657-4302676 | None:intergenic | 45.0% |
| CAGCGATGAGGTAAAGAGCA+AGG | + | chr8.3:4303040-4303059 | MS.gene57637:CDS | 50.0% | |
| CGATGAGGTAAAGAGCAAGG+TGG | + | chr8.3:4303043-4303062 | MS.gene57637:CDS | 50.0% | |
| CTCTTTACCTCATCGCTGAC+CGG | - | chr8.3:4303038-4303057 | None:intergenic | 50.0% | |
| GAAGCGAGAAGCTAGAGCTA+TGG | - | chr8.3:4302628-4302647 | None:intergenic | 50.0% | |
| GAGTTGAGGCTGAGTGAACT+CGG | - | chr8.3:4302799-4302818 | None:intergenic | 50.0% | |
| GTTCTTGGTTGTAGTGACGG+AGG | - | chr8.3:4302740-4302759 | None:intergenic | 50.0% | |
| TCTTTACCTCATCGCTGACC+GGG | - | chr8.3:4303037-4303056 | None:intergenic | 50.0% | |
| ! | GACGGTTTTCGAACGAGTTG+AGG | - | chr8.3:4302936-4302955 | None:intergenic | 50.0% |
| ! | GCTCTAGCTTCTCGCTTCAA+TGG | + | chr8.3:4302630-4302649 | MS.gene57637:CDS | 50.0% |
| !!! | TTTTCAGCTTCGGGTTGTCC+TGG | - | chr8.3:4303002-4303021 | None:intergenic | 50.0% |
| TCGGACTCTGACTCTACCTC+CGG | - | chr8.3:4302780-4302799 | None:intergenic | 55.0% | |
| CGCTGACCGGGTTCAGAGAT+GGG | - | chr8.3:4303025-4303044 | None:intergenic | 60.0% | |
| GGAGAGAAGAAGGCCAGCGA+CGG | + | chr8.3:4303127-4303146 | MS.gene57637:CDS | 60.0% | |
| TCGCTGACCGGGTTCAGAGA+TGG | - | chr8.3:4303026-4303045 | None:intergenic | 60.0% | |
| TCTGAACCCGGTCAGCGATG+AGG | + | chr8.3:4303028-4303047 | MS.gene57637:CDS | 60.0% | |
| GAGAAGAAGGCCAGCGACGG+CGG | + | chr8.3:4303130-4303149 | MS.gene57637:CDS | 65.0% | |
| AAGAAGGCCAGCGACGGCGG+CGG | + | chr8.3:4303133-4303152 | MS.gene57637:CDS | 70.0% | |
| ! | CTCCTCGCCGCCGCCGTCGC+TGG | - | chr8.3:4303143-4303162 | None:intergenic | 85.0% |
| ! | GGCCAGCGACGGCGGCGGCG+AGG | + | chr8.3:4303138-4303157 | MS.gene57637:CDS | 90.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 4302546 | 4303292 | 4302546 | ID=MS.gene57637 |
| chr8.3 | mRNA | 4302546 | 4303292 | 4302546 | ID=MS.gene57637.t1;Parent=MS.gene57637 |
| chr8.3 | exon | 4302546 | 4303292 | 4302546 | ID=MS.gene57637.t1.exon1;Parent=MS.gene57637.t1 |
| chr8.3 | CDS | 4302546 | 4303292 | 4302546 | ID=cds.MS.gene57637.t1;Parent=MS.gene57637.t1 |
| Gene Sequence |
| Protein sequence |
