Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57640.t1 | XP_013458484.1 | 67 | 221 | 41 | 3 | 1 | 221 | 1 | 189 | 2.50E-40 | 175.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57640.t1 | I3SL07 | 67.0 | 221 | 41 | 3 | 1 | 221 | 1 | 189 | 1.8e-40 | 175.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051864 | MS.gene57640 | 0.924615 | 5.56E-90 | -1.69E-46 |
| MS.gene061106 | MS.gene57640 | -0.811444 | 6.98E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57640.t1 | MTR_4g126170 | 75.446 | 224 | 19 | 5 | 1 | 222 | 1 | 190 | 7.60e-96 | 278 |
| MS.gene57640.t1 | MTR_4g126210 | 49.814 | 269 | 58 | 11 | 5 | 221 | 1 | 244 | 4.02e-52 | 169 |
| MS.gene57640.t1 | MTR_2g039320 | 29.911 | 224 | 119 | 7 | 17 | 222 | 16 | 219 | 1.26e-12 | 65.1 |
| MS.gene57640.t1 | MTR_1g016100 | 48.276 | 58 | 30 | 0 | 163 | 220 | 242 | 299 | 4.57e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57640.t1 | AT2G26110 | 31.179 | 263 | 119 | 8 | 13 | 219 | 44 | 300 | 4.38e-20 | 87.0 |
Find 44 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCGGACTCGGTCTCGGACTC+TGG | 0.364487 | 8.3:-4315522 | None:intergenic |
| ATCTTTGTTAACCTTGTCAT+TGG | 0.365409 | 8.3:+4315366 | MS.gene57640:CDS |
| AGTGTTCAATGACCTTTGAT+TGG | 0.386647 | 8.3:+4315748 | MS.gene57640:CDS |
| GACTTGACCGGTTCTGTAGA+TGG | 0.389512 | 8.3:-4315645 | None:intergenic |
| CTCCTCGCCATTGCAGTCGC+TGG | 0.396284 | 8.3:-4315810 | None:intergenic |
| ACTTGACCGGTTCTGTAGAT+GGG | 0.415711 | 8.3:-4315644 | None:intergenic |
| TCGGTCTCGGACTCTGGCTC+AGG | 0.437676 | 8.3:-4315516 | None:intergenic |
| GTGTTCAATGACCTTTGATT+GGG | 0.442400 | 8.3:+4315749 | MS.gene57640:CDS |
| GTTTCAGATTCTGTTTGTGC+TGG | 0.457165 | 8.3:-4315621 | None:intergenic |
| TAACCAATGTTGGCTCGTTA+TGG | 0.483691 | 8.3:-4315488 | None:intergenic |
| GAAATTATTACTTGCTGCTA+TGG | 0.484982 | 8.3:-4315304 | None:intergenic |
| AGTAGACTCGGACTCGGTCT+CGG | 0.491349 | 8.3:-4315529 | None:intergenic |
| ACATGTTTAAGAAGCAATTG+AGG | 0.499011 | 8.3:+4315895 | MS.gene57640:CDS |
| CTGAGATTGTGTGTTTGTTG+AGG | 0.500280 | 8.3:-4315450 | None:intergenic |
| TGAAACCCCATCTACAGAAC+CGG | 0.508418 | 8.3:+4315638 | MS.gene57640:CDS |
| TTCTTCTTCCTCCCAATCAA+AGG | 0.514922 | 8.3:-4315760 | None:intergenic |
| TGAGAATCACAATCGTGGTT+CGG | 0.515295 | 8.3:-4315672 | None:intergenic |
| GGAAGAAGAAGATGAAGAAA+CGG | 0.521334 | 8.3:+4315773 | MS.gene57640:CDS |
| TTCAATGACCTTTGATTGGG+AGG | 0.540849 | 8.3:+4315752 | MS.gene57640:CDS |
| TCAGGTTGTGTAACCAATGT+TGG | 0.542423 | 8.3:-4315498 | None:intergenic |
| ACAAAGATGAAAAGAGGAGA+AGG | 0.545362 | 8.3:-4315351 | None:intergenic |
| AAGGAGGCCAGCGACTGCAA+TGG | 0.559533 | 8.3:+4315803 | MS.gene57640:CDS |
| ATGAAGAAACGGTGGAGCGA+AGG | 0.562197 | 8.3:+4315784 | MS.gene57640:CDS |
| AAGAAACGGTGGAGCGAAGG+AGG | 0.565948 | 8.3:+4315787 | MS.gene57640:CDS |
| CTTGACCGGTTCTGTAGATG+GGG | 0.566562 | 8.3:-4315643 | None:intergenic |
| ATGTTGGCTCGTTATGGTGG+TGG | 0.574929 | 8.3:-4315482 | None:intergenic |
| TGGTTCGGATCCGACTTGAC+CGG | 0.581198 | 8.3:-4315657 | None:intergenic |
| AGGTTAACAAAGATGAAAAG+AGG | 0.582940 | 8.3:-4315357 | None:intergenic |
| AGAAGCAATTGAGGTTGCAG+AGG | 0.592934 | 8.3:+4315904 | MS.gene57640:CDS |
| CCAATGTTGGCTCGTTATGG+TGG | 0.593481 | 8.3:-4315485 | None:intergenic |
| ATCTACAGAACCGGTCAAGT+CGG | 0.601074 | 8.3:+4315647 | MS.gene57640:CDS |
| GGGTTGAGTAGACTCGGACT+CGG | 0.601445 | 8.3:-4315535 | None:intergenic |
| CGATTGTGATTCTCATGATG+AGG | 0.604792 | 8.3:+4315680 | MS.gene57640:CDS |
| GGCCAGCGACTGCAATGGCG+AGG | 0.605127 | 8.3:+4315808 | MS.gene57640:CDS |
| CCACCATAACGAGCCAACAT+TGG | 0.606919 | 8.3:+4315485 | MS.gene57640:CDS |
| GAGTTGGGGTTGAGTAGACT+CGG | 0.620169 | 8.3:-4315541 | None:intergenic |
| ATTTCATAGCCACATGCATG+TGG | 0.622525 | 8.3:+4315322 | MS.gene57640:CDS |
| AGAAGAAGATGAAGAAACGG+TGG | 0.628304 | 8.3:+4315776 | MS.gene57640:CDS |
| AGGTGTAAACCACATGCATG+TGG | 0.660037 | 8.3:-4315331 | None:intergenic |
| GAAGCGAGAAACGATAGCAA+TGG | 0.660890 | 8.3:-4315391 | None:intergenic |
| AAGACTCGCTGTAACAGTGA+AGG | 0.667221 | 8.3:-4315573 | None:intergenic |
| CATCATGAGAATCACAATCG+TGG | 0.680976 | 8.3:-4315677 | None:intergenic |
| TAGCAATGGTACCAATGACA+AGG | 0.697487 | 8.3:-4315377 | None:intergenic |
| TGAGGAGAATAGAAACAAAG+CGG | 0.717010 | 8.3:+4315698 | MS.gene57640:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACATGTTTAAGAAGCAATTG+AGG | + | chr8.3:4315895-4315914 | MS.gene57640:CDS | 30.0% | |
| AGGTTAACAAAGATGAAAAG+AGG | - | chr8.3:4315360-4315379 | None:intergenic | 30.0% | |
| ATCTTTGTTAACCTTGTCAT+TGG | + | chr8.3:4315366-4315385 | MS.gene57640:CDS | 30.0% | |
| ! | GAAATTATTACTTGCTGCTA+TGG | - | chr8.3:4315307-4315326 | None:intergenic | 30.0% |
| !!! | AGGTTGTTTTTGTTGAGTTT+TGG | - | chr8.3:4315433-4315452 | None:intergenic | 30.0% |
| !!! | GGTTGTTTTTGTTGAGTTTT+GGG | - | chr8.3:4315432-4315451 | None:intergenic | 30.0% |
| ACAAAGATGAAAAGAGGAGA+AGG | - | chr8.3:4315354-4315373 | None:intergenic | 35.0% | |
| AGTGTTCAATGACCTTTGAT+TGG | + | chr8.3:4315748-4315767 | MS.gene57640:CDS | 35.0% | |
| GGAAGAAGAAGATGAAGAAA+CGG | + | chr8.3:4315773-4315792 | MS.gene57640:CDS | 35.0% | |
| GTGTTCAATGACCTTTGATT+GGG | + | chr8.3:4315749-4315768 | MS.gene57640:CDS | 35.0% | |
| TGAGGAGAATAGAAACAAAG+CGG | + | chr8.3:4315698-4315717 | MS.gene57640:CDS | 35.0% | |
| !!! | TGAAGGTTTTAGAGTGAGTT+GGG | - | chr8.3:4315559-4315578 | None:intergenic | 35.0% |
| AGAAGAAGATGAAGAAACGG+TGG | + | chr8.3:4315776-4315795 | MS.gene57640:CDS | 40.0% | |
| ATTTCATAGCCACATGCATG+TGG | + | chr8.3:4315322-4315341 | MS.gene57640:CDS | 40.0% | |
| CATCATGAGAATCACAATCG+TGG | - | chr8.3:4315680-4315699 | None:intergenic | 40.0% | |
| CGATTGTGATTCTCATGATG+AGG | + | chr8.3:4315680-4315699 | MS.gene57640:CDS | 40.0% | |
| GTTTCAGATTCTGTTTGTGC+TGG | - | chr8.3:4315624-4315643 | None:intergenic | 40.0% | |
| TAGCAATGGTACCAATGACA+AGG | - | chr8.3:4315380-4315399 | None:intergenic | 40.0% | |
| TGAGAATCACAATCGTGGTT+CGG | - | chr8.3:4315675-4315694 | None:intergenic | 40.0% | |
| TTCAATGACCTTTGATTGGG+AGG | + | chr8.3:4315752-4315771 | MS.gene57640:CDS | 40.0% | |
| TTCTTCTTCCTCCCAATCAA+AGG | - | chr8.3:4315763-4315782 | None:intergenic | 40.0% | |
| ! | CTGAGATTGTGTGTTTGTTG+AGG | - | chr8.3:4315453-4315472 | None:intergenic | 40.0% |
| ! | TCAGGTTGTGTAACCAATGT+TGG | - | chr8.3:4315501-4315520 | None:intergenic | 40.0% |
| !! | TAACCAATGTTGGCTCGTTA+TGG | - | chr8.3:4315491-4315510 | None:intergenic | 40.0% |
| !!! | GAAGGTTTTAGAGTGAGTTG+GGG | - | chr8.3:4315558-4315577 | None:intergenic | 40.0% |
| !!! | GTGAAGGTTTTAGAGTGAGT+TGG | - | chr8.3:4315560-4315579 | None:intergenic | 40.0% |
| AAGACTCGCTGTAACAGTGA+AGG | - | chr8.3:4315576-4315595 | None:intergenic | 45.0% | |
| AGAAGCAATTGAGGTTGCAG+AGG | + | chr8.3:4315904-4315923 | MS.gene57640:CDS | 45.0% | |
| AGGTGTAAACCACATGCATG+TGG | - | chr8.3:4315334-4315353 | None:intergenic | 45.0% | |
| ATCTACAGAACCGGTCAAGT+CGG | + | chr8.3:4315647-4315666 | MS.gene57640:CDS | 45.0% | |
| GAAGCGAGAAACGATAGCAA+TGG | - | chr8.3:4315394-4315413 | None:intergenic | 45.0% | |
| TGAAACCCCATCTACAGAAC+CGG | + | chr8.3:4315638-4315657 | MS.gene57640:CDS | 45.0% | |
| ! | ACTTGACCGGTTCTGTAGAT+GGG | - | chr8.3:4315647-4315666 | None:intergenic | 45.0% |
| ATGAAGAAACGGTGGAGCGA+AGG | + | chr8.3:4315784-4315803 | MS.gene57640:CDS | 50.0% | |
| CCACCATAACGAGCCAACAT+TGG | + | chr8.3:4315485-4315504 | MS.gene57640:CDS | 50.0% | |
| GAGTTGGGGTTGAGTAGACT+CGG | - | chr8.3:4315544-4315563 | None:intergenic | 50.0% | |
| ! | CTTGACCGGTTCTGTAGATG+GGG | - | chr8.3:4315646-4315665 | None:intergenic | 50.0% |
| ! | GACTTGACCGGTTCTGTAGA+TGG | - | chr8.3:4315648-4315667 | None:intergenic | 50.0% |
| !! | ATGTTGGCTCGTTATGGTGG+TGG | - | chr8.3:4315485-4315504 | None:intergenic | 50.0% |
| !! | CCAATGTTGGCTCGTTATGG+TGG | - | chr8.3:4315488-4315507 | None:intergenic | 50.0% |
| AAGAAACGGTGGAGCGAAGG+AGG | + | chr8.3:4315787-4315806 | MS.gene57640:CDS | 55.0% | |
| GGGTTGAGTAGACTCGGACT+CGG | - | chr8.3:4315538-4315557 | None:intergenic | 55.0% | |
| ! | AGTAGACTCGGACTCGGTCT+CGG | - | chr8.3:4315532-4315551 | None:intergenic | 55.0% |
| ! | TGGTTCGGATCCGACTTGAC+CGG | - | chr8.3:4315660-4315679 | None:intergenic | 55.0% |
| AAGGAGGCCAGCGACTGCAA+TGG | + | chr8.3:4315803-4315822 | MS.gene57640:CDS | 60.0% | |
| CTCCTCGCCATTGCAGTCGC+TGG | - | chr8.3:4315813-4315832 | None:intergenic | 65.0% | |
| ! | TCGGACTCGGTCTCGGACTC+TGG | - | chr8.3:4315525-4315544 | None:intergenic | 65.0% |
| ! | TCGGTCTCGGACTCTGGCTC+AGG | - | chr8.3:4315519-4315538 | None:intergenic | 65.0% |
| GGCCAGCGACTGCAATGGCG+AGG | + | chr8.3:4315808-4315827 | MS.gene57640:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 4315294 | 4315962 | 4315294 | ID=MS.gene57640 |
| chr8.3 | mRNA | 4315294 | 4315962 | 4315294 | ID=MS.gene57640.t1;Parent=MS.gene57640 |
| chr8.3 | exon | 4315294 | 4315962 | 4315294 | ID=MS.gene57640.t1.exon1;Parent=MS.gene57640.t1 |
| chr8.3 | CDS | 4315294 | 4315962 | 4315294 | ID=cds.MS.gene57640.t1;Parent=MS.gene57640.t1 |
| Gene Sequence |
| Protein sequence |
