Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051864.t1 | XP_013458484.1 | 57.6 | 297 | 18 | 5 | 1 | 297 | 1 | 189 | 7.70E-45 | 191 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051864.t1 | I3SL07 | 57.6 | 297 | 18 | 5 | 1 | 297 | 1 | 189 | 5.6e-45 | 191.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051864 | MS.gene064509 | -0.801245 | 9.86E-49 | -1.69E-46 |
| MS.gene051864 | MS.gene065052 | -0.825054 | 5.78E-54 | -1.69E-46 |
| MS.gene051864 | MS.gene35546 | -0.80024 | 1.58E-48 | -1.69E-46 |
| MS.gene051864 | MS.gene35794 | -0.828516 | 8.61E-55 | -1.69E-46 |
| MS.gene051864 | MS.gene35795 | -0.816909 | 4.34E-52 | -1.69E-46 |
| MS.gene051864 | MS.gene35853 | -0.822 | 2.99E-53 | -1.69E-46 |
| MS.gene051864 | MS.gene35931 | -0.823165 | 1.60E-53 | -1.69E-46 |
| MS.gene051864 | MS.gene49756 | -0.837755 | 4.31E-57 | -1.69E-46 |
| MS.gene051864 | MS.gene57587 | 0.91408 | 2.92E-84 | -1.69E-46 |
| MS.gene051864 | MS.gene57588 | 0.902113 | 1.35E-78 | -1.69E-46 |
| MS.gene051864 | MS.gene57590 | 0.891716 | 3.08E-74 | -1.69E-46 |
| MS.gene051864 | MS.gene57640 | 0.924615 | 5.56E-90 | -1.69E-46 |
| MS.gene051864 | MS.gene70127 | -0.840458 | 8.59E-58 | -1.69E-46 |
| MS.gene051864 | MS.gene74969 | -0.801801 | 7.59E-49 | -1.69E-46 |
| MS.gene051864 | MS.gene75365 | -0.80992 | 1.49E-50 | -1.69E-46 |
| MS.gene051864 | MS.gene82356 | 0.826779 | 2.25E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051864.t1 | MTR_4g126170 | 69.792 | 192 | 22 | 5 | 109 | 298 | 33 | 190 | 8.32e-65 | 202 |
| MS.gene051864.t1 | MTR_4g126170 | 83.478 | 115 | 10 | 3 | 1 | 113 | 1 | 108 | 3.30e-57 | 182 |
| MS.gene051864.t1 | MTR_4g126210 | 51.447 | 311 | 66 | 14 | 5 | 297 | 1 | 244 | 7.82e-62 | 196 |
| MS.gene051864.t1 | MTR_1g016100 | 48.276 | 58 | 30 | 0 | 239 | 296 | 242 | 299 | 8.18e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051864.t1 | AT2G26110 | 26.959 | 319 | 135 | 9 | 13 | 295 | 44 | 300 | 1.44e-16 | 79.0 |
| MS.gene051864.t1 | AT5G56980 | 27.586 | 377 | 162 | 17 | 17 | 295 | 10 | 373 | 9.48e-14 | 71.2 |
Find 58 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCGGACTCGGTCTCGGACTC+TGG | 0.364487 | 8.2:-6402646 | None:intergenic |
| ATCTTTGTTAACCTTGTCAT+TGG | 0.365409 | 8.2:+6402262 | MS.gene051864:CDS |
| AGTGTTCAATGACCTTTGAT+TGG | 0.386647 | 8.2:+6402872 | MS.gene051864:CDS |
| GACTTGACCGGTTCTGTAGA+TGG | 0.389512 | 8.2:-6402769 | None:intergenic |
| CCCCTCGCCATTGCAGTCGC+TGG | 0.396284 | 8.2:-6402934 | None:intergenic |
| ACTTGACCGGTTCTGTAGAT+GGG | 0.415711 | 8.2:-6402768 | None:intergenic |
| TCGGTCTCGGACTCTGGCTC+AGG | 0.437676 | 8.2:-6402640 | None:intergenic |
| TACCCAATGTTGGCTCGTTA+TGG | 0.439407 | 8.2:-6402384 | None:intergenic |
| GGTTGAGTAGACTCGGACTC+TGG | 0.440049 | 8.2:-6402418 | None:intergenic |
| GTGTTCAATGACCTTTGATT+GGG | 0.442400 | 8.2:+6402873 | MS.gene051864:CDS |
| GTTTCAGATTCTGTTTGTGC+TGG | 0.446835 | 8.2:-6402505 | None:intergenic |
| GAAATTATTACTTGCTGCTA+TGG | 0.447524 | 8.2:-6402200 | None:intergenic |
| GTTTCAGATTCTGTTTGTGC+TGG | 0.457165 | 8.2:-6402745 | None:intergenic |
| TAACCAATGTTGGCTCGTTA+TGG | 0.483691 | 8.2:-6402612 | None:intergenic |
| CTGAGATTGTGTGTTTGATG+AGG | 0.486552 | 8.2:-6402346 | None:intergenic |
| GTAGACTCGGACTCTGGCTC+AGG | 0.489096 | 8.2:-6402412 | None:intergenic |
| AGTAGACTCGGACTCGGTCT+CGG | 0.491349 | 8.2:-6402653 | None:intergenic |
| ACATGTTTAAGAAGCAATTG+AGG | 0.499011 | 8.2:+6403019 | MS.gene051864:CDS |
| CTGAGATTGTGTGTTTGTTG+AGG | 0.500280 | 8.2:-6402574 | None:intergenic |
| CACCATAACGAGCCAACATT+GGG | 0.504691 | 8.2:+6402382 | MS.gene051864:CDS |
| TGAAACCCCATCTACAGAAC+CGG | 0.508418 | 8.2:+6402762 | MS.gene051864:CDS |
| TTCTTCTTCCTCCCAATCAA+AGG | 0.514922 | 8.2:-6402884 | None:intergenic |
| TGAGAATCACAATCGTGGTT+CGG | 0.515295 | 8.2:-6402796 | None:intergenic |
| CCACCATAACGAGCCAACAT+TGG | 0.518538 | 8.2:+6402381 | MS.gene051864:CDS |
| GGAAGAAGAAGATGAAGAAA+CGG | 0.521334 | 8.2:+6402897 | MS.gene051864:CDS |
| TTCAATGACCTTTGATTGGG+AGG | 0.540849 | 8.2:+6402876 | MS.gene051864:CDS |
| TCAGGTTGTGTAACCAATGT+TGG | 0.542423 | 8.2:-6402622 | None:intergenic |
| ACAAAGATGAAAAGAGGAGA+AGG | 0.545362 | 8.2:-6402247 | None:intergenic |
| GCCAGCGACTGCAATGGCGA+GGG | 0.549742 | 8.2:+6402933 | MS.gene051864:CDS |
| TCAGGTTGTGTACCCAATGT+TGG | 0.553871 | 8.2:-6402394 | None:intergenic |
| GGCCAGCGACTGCAATGGCG+AGG | 0.557035 | 8.2:+6402932 | MS.gene051864:CDS |
| AAGGAGGCCAGCGACTGCAA+TGG | 0.559533 | 8.2:+6402927 | MS.gene051864:CDS |
| ATGAAGAAACGGTGGAGCGA+AGG | 0.562197 | 8.2:+6402908 | MS.gene051864:CDS |
| AAGAAACGGTGGAGCGAAGG+AGG | 0.565948 | 8.2:+6402911 | MS.gene051864:CDS |
| CTTGACCGGTTCTGTAGATG+GGG | 0.566562 | 8.2:-6402767 | None:intergenic |
| ATGTTGGCTCGTTATGGTGG+TGG | 0.574929 | 8.2:-6402378 | None:intergenic |
| ATGTTGGCTCGTTATGGTGG+TGG | 0.574929 | 8.2:-6402606 | None:intergenic |
| TGGTTCGGATCCGACTTGAC+CGG | 0.581198 | 8.2:-6402781 | None:intergenic |
| AGGTTAACAAAGATGAAAAG+AGG | 0.582940 | 8.2:-6402253 | None:intergenic |
| AGAAGCAATTGAGGTTGCAG+AGG | 0.592934 | 8.2:+6403028 | MS.gene051864:CDS |
| CCAATGTTGGCTCGTTATGG+TGG | 0.593481 | 8.2:-6402609 | None:intergenic |
| CCAGCGACTGCAATGGCGAG+GGG | 0.593879 | 8.2:+6402934 | MS.gene051864:CDS |
| ATCTACAGAACCGGTCAAGT+CGG | 0.601074 | 8.2:+6402771 | MS.gene051864:CDS |
| GGGTTGAGTAGACTCGGACT+CGG | 0.601445 | 8.2:-6402659 | None:intergenic |
| CCAATGTTGGCTCGTTATGG+TGG | 0.604599 | 8.2:-6402381 | None:intergenic |
| CGATTGTGATTCTCATGATG+AGG | 0.604792 | 8.2:+6402804 | MS.gene051864:CDS |
| CCACCATAACGAGCCAACAT+TGG | 0.606919 | 8.2:+6402609 | MS.gene051864:CDS |
| GAGTTGGGGTTGAGTAGACT+CGG | 0.620169 | 8.2:-6402425 | None:intergenic |
| GAGTTGGGGTTGAGTAGACT+CGG | 0.620169 | 8.2:-6402665 | None:intergenic |
| ATTTCATAGCCACATGCATG+TGG | 0.622525 | 8.2:+6402218 | MS.gene051864:CDS |
| AGAAGAAGATGAAGAAACGG+TGG | 0.628304 | 8.2:+6402900 | MS.gene051864:CDS |
| AGGTGTAAACCACATGCATG+TGG | 0.660037 | 8.2:-6402227 | None:intergenic |
| GAAGCGAGAAACGATAGCAA+TGG | 0.660890 | 8.2:-6402287 | None:intergenic |
| AAGACTCGCTGTAACAGTGA+AGG | 0.667221 | 8.2:-6402457 | None:intergenic |
| AAGACTCGCTGTAACAGTGA+AGG | 0.667221 | 8.2:-6402697 | None:intergenic |
| CATCATGAGAATCACAATCG+TGG | 0.680976 | 8.2:-6402801 | None:intergenic |
| TAGCAATGGTACCAATGACA+AGG | 0.697487 | 8.2:-6402273 | None:intergenic |
| TGAGGAGAATAGAAACAAAG+CGG | 0.717010 | 8.2:+6402822 | MS.gene051864:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACATGTTTAAGAAGCAATTG+AGG | + | chr8.2:6403019-6403038 | MS.gene051864:CDS | 30.0% | |
| AGGTTAACAAAGATGAAAAG+AGG | - | chr8.2:6402256-6402275 | None:intergenic | 30.0% | |
| ATCTTTGTTAACCTTGTCAT+TGG | + | chr8.2:6402262-6402281 | MS.gene051864:CDS | 30.0% | |
| ! | GAAATTATTACTTGCTGCTA+TGG | - | chr8.2:6402203-6402222 | None:intergenic | 30.0% |
| !!! | AGGTTGTTTTTGTTGAGTTT+TGG | - | chr8.2:6402557-6402576 | None:intergenic | 30.0% |
| !!! | GGTTGTTTTTGTTGAGTTTT+GGG | - | chr8.2:6402556-6402575 | None:intergenic | 30.0% |
| !!! | TTGATGAGGTTGTTTTTGTT+GGG | - | chr8.2:6402335-6402354 | None:intergenic | 30.0% |
| !!! | TTTGATGAGGTTGTTTTTGT+TGG | - | chr8.2:6402336-6402355 | None:intergenic | 30.0% |
| ACAAAGATGAAAAGAGGAGA+AGG | - | chr8.2:6402250-6402269 | None:intergenic | 35.0% | |
| AGTGTTCAATGACCTTTGAT+TGG | + | chr8.2:6402872-6402891 | MS.gene051864:CDS | 35.0% | |
| GGAAGAAGAAGATGAAGAAA+CGG | + | chr8.2:6402897-6402916 | MS.gene051864:CDS | 35.0% | |
| GTGTTCAATGACCTTTGATT+GGG | + | chr8.2:6402873-6402892 | MS.gene051864:CDS | 35.0% | |
| TGAGGAGAATAGAAACAAAG+CGG | + | chr8.2:6402822-6402841 | MS.gene051864:CDS | 35.0% | |
| !!! | AGGTTGTTTTTGTTGGGTTT+TGG | - | chr8.2:6402329-6402348 | None:intergenic | 35.0% |
| !!! | GGTTGTTTTTGTTGGGTTTT+GGG | - | chr8.2:6402328-6402347 | None:intergenic | 35.0% |
| !!! | TGAAGGTTTTAGAGTGAGTT+GGG | - | chr8.2:6402443-6402462 | None:intergenic | 35.0% |
| AGAAGAAGATGAAGAAACGG+TGG | + | chr8.2:6402900-6402919 | MS.gene051864:CDS | 40.0% | |
| ATTTCATAGCCACATGCATG+TGG | + | chr8.2:6402218-6402237 | MS.gene051864:CDS | 40.0% | |
| CATCATGAGAATCACAATCG+TGG | - | chr8.2:6402804-6402823 | None:intergenic | 40.0% | |
| CGATTGTGATTCTCATGATG+AGG | + | chr8.2:6402804-6402823 | MS.gene051864:CDS | 40.0% | |
| CTGAGATTGTGTGTTTGATG+AGG | - | chr8.2:6402349-6402368 | None:intergenic | 40.0% | |
| GTTTCAGATTCTGTTTGTGC+TGG | - | chr8.2:6402508-6402527 | None:intergenic | 40.0% | |
| TAGCAATGGTACCAATGACA+AGG | - | chr8.2:6402276-6402295 | None:intergenic | 40.0% | |
| TGAGAATCACAATCGTGGTT+CGG | - | chr8.2:6402799-6402818 | None:intergenic | 40.0% | |
| TTCAATGACCTTTGATTGGG+AGG | + | chr8.2:6402876-6402895 | MS.gene051864:CDS | 40.0% | |
| TTCTTCTTCCTCCCAATCAA+AGG | - | chr8.2:6402887-6402906 | None:intergenic | 40.0% | |
| ! | CTGAGATTGTGTGTTTGTTG+AGG | - | chr8.2:6402577-6402596 | None:intergenic | 40.0% |
| ! | TCAGGTTGTGTAACCAATGT+TGG | - | chr8.2:6402625-6402644 | None:intergenic | 40.0% |
| !! | TAACCAATGTTGGCTCGTTA+TGG | - | chr8.2:6402615-6402634 | None:intergenic | 40.0% |
| !!! | GAAGGTTTTAGAGTGAGTTG+GGG | - | chr8.2:6402442-6402461 | None:intergenic | 40.0% |
| !!! | GTGAAGGTTTTAGAGTGAGT+TGG | - | chr8.2:6402444-6402463 | None:intergenic | 40.0% |
| AAGACTCGCTGTAACAGTGA+AGG | - | chr8.2:6402460-6402479 | None:intergenic | 45.0% | |
| AGAAGCAATTGAGGTTGCAG+AGG | + | chr8.2:6403028-6403047 | MS.gene051864:CDS | 45.0% | |
| AGGTGTAAACCACATGCATG+TGG | - | chr8.2:6402230-6402249 | None:intergenic | 45.0% | |
| ATCTACAGAACCGGTCAAGT+CGG | + | chr8.2:6402771-6402790 | MS.gene051864:CDS | 45.0% | |
| CACCATAACGAGCCAACATT+GGG | + | chr8.2:6402382-6402401 | MS.gene051864:CDS | 45.0% | |
| GAAGCGAGAAACGATAGCAA+TGG | - | chr8.2:6402290-6402309 | None:intergenic | 45.0% | |
| TGAAACCCCATCTACAGAAC+CGG | + | chr8.2:6402762-6402781 | MS.gene051864:CDS | 45.0% | |
| ! | ACTTGACCGGTTCTGTAGAT+GGG | - | chr8.2:6402771-6402790 | None:intergenic | 45.0% |
| ! | TCAGGTTGTGTACCCAATGT+TGG | - | chr8.2:6402397-6402416 | None:intergenic | 45.0% |
| !! | TACCCAATGTTGGCTCGTTA+TGG | - | chr8.2:6402387-6402406 | None:intergenic | 45.0% |
| ATGAAGAAACGGTGGAGCGA+AGG | + | chr8.2:6402908-6402927 | MS.gene051864:CDS | 50.0% | |
| CCACCATAACGAGCCAACAT+TGG | + | chr8.2:6402381-6402400 | MS.gene051864:CDS | 50.0% | |
| GAGTTGGGGTTGAGTAGACT+CGG | - | chr8.2:6402428-6402447 | None:intergenic | 50.0% | |
| ! | CTTGACCGGTTCTGTAGATG+GGG | - | chr8.2:6402770-6402789 | None:intergenic | 50.0% |
| ! | GACTTGACCGGTTCTGTAGA+TGG | - | chr8.2:6402772-6402791 | None:intergenic | 50.0% |
| !! | ATGTTGGCTCGTTATGGTGG+TGG | - | chr8.2:6402381-6402400 | None:intergenic | 50.0% |
| !! | CCAATGTTGGCTCGTTATGG+TGG | - | chr8.2:6402384-6402403 | None:intergenic | 50.0% |
| AAGAAACGGTGGAGCGAAGG+AGG | + | chr8.2:6402911-6402930 | MS.gene051864:CDS | 55.0% | |
| GGGTTGAGTAGACTCGGACT+CGG | - | chr8.2:6402422-6402441 | None:intergenic | 55.0% | |
| GGTTGAGTAGACTCGGACTC+TGG | - | chr8.2:6402421-6402440 | None:intergenic | 55.0% | |
| ! | AGTAGACTCGGACTCGGTCT+CGG | - | chr8.2:6402656-6402675 | None:intergenic | 55.0% |
| ! | TGGTTCGGATCCGACTTGAC+CGG | - | chr8.2:6402784-6402803 | None:intergenic | 55.0% |
| AAGGAGGCCAGCGACTGCAA+TGG | + | chr8.2:6402927-6402946 | MS.gene051864:CDS | 60.0% | |
| ! | GTAGACTCGGACTCTGGCTC+AGG | - | chr8.2:6402415-6402434 | None:intergenic | 60.0% |
| CCAGCGACTGCAATGGCGAG+GGG | + | chr8.2:6402934-6402953 | MS.gene051864:CDS | 65.0% | |
| GCCAGCGACTGCAATGGCGA+GGG | + | chr8.2:6402933-6402952 | MS.gene051864:CDS | 65.0% | |
| ! | TCGGACTCGGTCTCGGACTC+TGG | - | chr8.2:6402649-6402668 | None:intergenic | 65.0% |
| ! | TCGGTCTCGGACTCTGGCTC+AGG | - | chr8.2:6402643-6402662 | None:intergenic | 65.0% |
| CCCCTCGCCATTGCAGTCGC+TGG | - | chr8.2:6402937-6402956 | None:intergenic | 70.0% | |
| GGCCAGCGACTGCAATGGCG+AGG | + | chr8.2:6402932-6402951 | MS.gene051864:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 6402190 | 6403086 | 6402190 | ID=MS.gene051864 |
| chr8.2 | mRNA | 6402190 | 6403086 | 6402190 | ID=MS.gene051864.t1;Parent=MS.gene051864 |
| chr8.2 | exon | 6402190 | 6403086 | 6402190 | ID=MS.gene051864.t1.exon1;Parent=MS.gene051864.t1 |
| chr8.2 | CDS | 6402190 | 6403086 | 6402190 | ID=cds.MS.gene051864.t1;Parent=MS.gene051864.t1 |
| Gene Sequence |
| Protein sequence |
