Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57641.t1 | XP_013458483.1 | 97.6 | 498 | 12 | 0 | 40 | 537 | 21 | 518 | 1.70E-289 | 1004.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57641.t1 | Q9LTS3 | 51.4 | 498 | 231 | 4 | 42 | 536 | 28 | 517 | 6.8e-149 | 528.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57641.t1 | A0A072URS8 | 97.6 | 498 | 12 | 0 | 40 | 537 | 21 | 518 | 1.3e-289 | 1004.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051863 | MS.gene57641 | 0.865718 | 4.82E-65 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57641.t1 | MTR_4g126160 | 97.590 | 498 | 12 | 0 | 40 | 537 | 21 | 518 | 0.0 | 1006 |
| MS.gene57641.t1 | MTR_2g039340 | 67.143 | 490 | 150 | 3 | 56 | 536 | 34 | 521 | 0.0 | 697 |
| MS.gene57641.t1 | MTR_4g126150 | 60.331 | 484 | 177 | 4 | 63 | 537 | 49 | 526 | 0.0 | 612 |
| MS.gene57641.t1 | MTR_2g039410 | 56.226 | 514 | 203 | 6 | 45 | 537 | 26 | 538 | 0.0 | 579 |
| MS.gene57641.t1 | MTR_3g112350 | 50.725 | 483 | 224 | 7 | 63 | 537 | 47 | 523 | 1.22e-166 | 483 |
| MS.gene57641.t1 | MTR_3g036100 | 46.791 | 483 | 237 | 8 | 63 | 537 | 71 | 541 | 5.76e-152 | 446 |
| MS.gene57641.t1 | MTR_4g044110 | 47.023 | 487 | 233 | 10 | 63 | 536 | 47 | 521 | 2.38e-149 | 439 |
| MS.gene57641.t1 | MTR_7g090920 | 46.218 | 476 | 232 | 8 | 73 | 536 | 73 | 536 | 3.29e-147 | 434 |
| MS.gene57641.t1 | MTR_1g015410 | 42.531 | 482 | 250 | 13 | 71 | 537 | 35 | 504 | 1.52e-122 | 369 |
| MS.gene57641.t1 | MTR_0005s0070 | 49.091 | 275 | 122 | 7 | 86 | 352 | 1 | 265 | 4.14e-79 | 249 |
| MS.gene57641.t1 | MTR_2g039360 | 43.678 | 87 | 48 | 1 | 452 | 537 | 77 | 163 | 7.61e-19 | 84.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57641.t1 | AT5G56970 | 51.406 | 498 | 231 | 4 | 42 | 536 | 28 | 517 | 0.0 | 526 |
| MS.gene57641.t1 | AT4G29740 | 50.823 | 486 | 219 | 4 | 66 | 537 | 45 | 524 | 3.12e-177 | 510 |
| MS.gene57641.t1 | AT2G19500 | 49.284 | 489 | 231 | 4 | 53 | 536 | 25 | 501 | 6.58e-174 | 500 |
| MS.gene57641.t1 | AT1G75450 | 48.104 | 501 | 233 | 9 | 55 | 536 | 28 | 520 | 2.78e-163 | 475 |
| MS.gene57641.t1 | AT1G75450 | 48.008 | 502 | 233 | 10 | 55 | 536 | 24 | 517 | 1.90e-161 | 470 |
| MS.gene57641.t1 | AT3G63440 | 47.689 | 476 | 228 | 8 | 73 | 536 | 60 | 526 | 3.31e-150 | 441 |
| MS.gene57641.t1 | AT2G41510 | 46.122 | 477 | 229 | 8 | 74 | 536 | 77 | 539 | 3.26e-147 | 435 |
| MS.gene57641.t1 | AT2G41510 | 46.025 | 478 | 230 | 8 | 73 | 536 | 138 | 601 | 1.99e-146 | 435 |
| MS.gene57641.t1 | AT4G29740 | 52.564 | 390 | 166 | 3 | 66 | 442 | 45 | 428 | 2.46e-142 | 417 |
| MS.gene57641.t1 | AT5G56970 | 51.733 | 404 | 184 | 4 | 42 | 442 | 28 | 423 | 9.12e-142 | 416 |
| MS.gene57641.t1 | AT5G21482 | 44.330 | 485 | 239 | 12 | 72 | 537 | 49 | 521 | 1.90e-128 | 385 |
| MS.gene57641.t1 | AT2G41510 | 46.966 | 379 | 178 | 7 | 73 | 442 | 76 | 440 | 1.12e-113 | 344 |
| MS.gene57641.t1 | AT4G29740 | 52.769 | 307 | 126 | 3 | 66 | 359 | 45 | 345 | 2.00e-106 | 323 |
Find 113 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCAACTGGGTTTGATAATT+TGG | 0.172846 | 8.3:+4333088 | MS.gene57641:CDS |
| ATTCTTCATGTGTTCTGTTT+AGG | 0.174158 | 8.3:-4333187 | None:intergenic |
| TCACTAGCCTCAACCGATTT+TGG | 0.182190 | 8.3:+4324600 | MS.gene57641:CDS |
| CCTCTTGCTGCTATAGTAAA+AGG | 0.182887 | 8.3:-4324717 | None:intergenic |
| AGAGTTATCGTCGTAATATT+TGG | 0.224291 | 8.3:-4332498 | None:intergenic |
| AATAATGAGCTTAGAAATAA+TGG | 0.225918 | 8.3:+4324807 | MS.gene57641:CDS |
| CTTGGAGGTCTAGGTCAATT+TGG | 0.234855 | 8.3:+4331863 | MS.gene57641:CDS |
| ATGCGAGGTTGATCAATTTC+TGG | 0.237168 | 8.3:-4332428 | None:intergenic |
| TTCTTCATGTGTTCTGTTTA+GGG | 0.237319 | 8.3:-4333186 | None:intergenic |
| TCAATGAAATTAAATGTTCT+TGG | 0.243318 | 8.3:-4332322 | None:intergenic |
| AAGGACTTACACCACTTTCT+TGG | 0.249326 | 8.3:+4324913 | MS.gene57641:CDS |
| GGGCAAGGGCATTCAGTTAA+TGG | 0.292683 | 8.3:+4324738 | MS.gene57641:CDS |
| ACTTACTTTGTTCGGTTCAT+TGG | 0.299489 | 8.3:-4332928 | None:intergenic |
| TTGGAGGTCTAGGTCAATTT+GGG | 0.302604 | 8.3:+4331864 | MS.gene57641:CDS |
| TGATGTAGATTATGCCATAT+TGG | 0.306461 | 8.3:-4332466 | None:intergenic |
| CATGAATTGGATGTTGTTAC+TGG | 0.312954 | 8.3:+4325029 | MS.gene57641:CDS |
| CTCTCTAATGCTGGCATTAG+TGG | 0.313714 | 8.3:+4324969 | MS.gene57641:CDS |
| AATTTCCAATGTTCATGAAT+TGG | 0.316418 | 8.3:+4325016 | MS.gene57641:CDS |
| TGGTTAGACCTATTTGTTCC+AGG | 0.317776 | 8.3:+4332820 | MS.gene57641:CDS |
| ACGTAGAAAATTTCTTCATT+AGG | 0.319059 | 8.3:-4333050 | None:intergenic |
| CACTAAGGGCACAAGGGCTT+TGG | 0.322463 | 8.3:+4332782 | MS.gene57641:CDS |
| GAACATTTAATTTCATTGAA+TGG | 0.325711 | 8.3:+4332326 | MS.gene57641:CDS |
| GACTGATTATTTGTATTTAT+CGG | 0.326686 | 8.3:+4324935 | MS.gene57641:CDS |
| AATTCACCCAATTATGTAGA+AGG | 0.351152 | 8.3:+4332362 | MS.gene57641:CDS |
| TACAGAATTGCAATATTTGT+TGG | 0.363574 | 8.3:-4333130 | None:intergenic |
| AGCATTTGGAGGATTTCTTT+TGG | 0.368836 | 8.3:-4324555 | None:intergenic |
| ACTTCCTTGGTAAACCAATA+TGG | 0.384782 | 8.3:+4332452 | MS.gene57641:CDS |
| TCTCAAAAGTATTCCATTTG+AGG | 0.386197 | 8.3:-4333226 | None:intergenic |
| AGTGGACAAAGCTTCCGATT+TGG | 0.388906 | 8.3:+4324987 | MS.gene57641:CDS |
| AAGGCAAAGTTAATAAACTA+TGG | 0.394526 | 8.3:+4324428 | MS.gene57641:CDS |
| GAAGAATGGGTGGAACAATA+TGG | 0.397648 | 8.3:+4333203 | MS.gene57641:CDS |
| CTTACTTTGTTCGGTTCATT+GGG | 0.402378 | 8.3:-4332927 | None:intergenic |
| CGGGGTCACGACTAAGCATT+TGG | 0.404940 | 8.3:-4324569 | None:intergenic |
| CTCTCAAGATCATGTTGATC+AGG | 0.418087 | 8.3:+4332517 | MS.gene57641:CDS |
| TATGCGGTTCTTGGAGGTCT+AGG | 0.425284 | 8.3:+4331854 | MS.gene57641:CDS |
| ACTAAGGGCACAAGGGCTTT+GGG | 0.426367 | 8.3:+4332783 | MS.gene57641:CDS |
| TTGCATGCAAGCCTTGAAAA+AGG | 0.427576 | 8.3:+4324894 | MS.gene57641:CDS |
| GTGAATTATGTGGCCAAAAT+CGG | 0.429011 | 8.3:-4324613 | None:intergenic |
| TCATGAACATTGGAAATTTG+AGG | 0.429894 | 8.3:-4325011 | None:intergenic |
| GGACAATCCATGACAAATGA+TGG | 0.430303 | 8.3:+4324759 | MS.gene57641:CDS |
| GTCCAAGAGCTATTCTTGCC+CGG | 0.443689 | 8.3:-4331895 | None:intergenic |
| ACAAATATTGCAATTCTGTA+AGG | 0.447727 | 8.3:+4333133 | MS.gene57641:CDS |
| GATATGTCATTAATAGAAGA+TGG | 0.452766 | 8.3:-4324660 | None:intergenic |
| TTTGGGATATCCCACATCCT+TGG | 0.453084 | 8.3:+4332800 | MS.gene57641:CDS |
| TGGAAATTTGAGGACCAAAT+CGG | 0.455857 | 8.3:-4325001 | None:intergenic |
| AATTCAGACATTCTAGATCC+TGG | 0.456400 | 8.3:-4332838 | None:intergenic |
| TGGAACAATATGGCCTCAAA+TGG | 0.459939 | 8.3:+4333213 | MS.gene57641:CDS |
| ATCGGTTGAGGCTAGTGAAA+TGG | 0.462882 | 8.3:-4324595 | None:intergenic |
| GATTATTTGTATTTATCGGT+TGG | 0.468139 | 8.3:+4324939 | MS.gene57641:CDS |
| TTACTTACCCACTTGGGTGC+AGG | 0.469378 | 8.3:-4331917 | None:intergenic |
| AAAATTCGTAAATCAATTGA+AGG | 0.472032 | 8.3:+4324409 | MS.gene57641:CDS |
| TAAACAGAACACATGAAGAA+TGG | 0.476873 | 8.3:+4333189 | MS.gene57641:CDS |
| CCAAATAAAATATTGTCACC+TGG | 0.478461 | 8.3:+4333269 | MS.gene57641:CDS |
| AGAAGTCGTTGTAAAGTAAA+CGG | 0.480233 | 8.3:-4332283 | None:intergenic |
| ATTTAATGAAGGTGTGTTCA+AGG | 0.481817 | 8.3:+4332858 | MS.gene57641:CDS |
| TTCTAGATCCTGGAACAAAT+AGG | 0.484678 | 8.3:-4332828 | None:intergenic |
| TTAATTTCTTGTAGGTAAAG+TGG | 0.485135 | 8.3:+4332258 | MS.gene57641:intron |
| ATAATGAGCTTAGAAATAAT+GGG | 0.488294 | 8.3:+4324808 | MS.gene57641:CDS |
| TCAACCTCGCATAACTTCCT+TGG | 0.491369 | 8.3:+4332439 | MS.gene57641:CDS |
| GAGCTTAGAAATAATGGGAA+TGG | 0.498772 | 8.3:+4324813 | MS.gene57641:CDS |
| AAACAGAACACATGAAGAAT+GGG | 0.503138 | 8.3:+4333190 | MS.gene57641:CDS |
| AATAGGTCTAACCAAGGATG+TGG | 0.503374 | 8.3:-4332811 | None:intergenic |
| CTCTTGCTGCTATAGTAAAA+GGG | 0.503657 | 8.3:-4324716 | None:intergenic |
| TATTTGTATTTATCGGTTGG+TGG | 0.507411 | 8.3:+4324942 | MS.gene57641:CDS |
| ACCCGGGCAAGAATAGCTCT+TGG | 0.507789 | 8.3:+4331893 | MS.gene57641:CDS |
| AACATCCAATTCATGAACAT+TGG | 0.509585 | 8.3:-4325021 | None:intergenic |
| CACAATCCCATCATTTGTCA+TGG | 0.510884 | 8.3:-4324766 | None:intergenic |
| GGTGGAACTCTCTCTAATGC+TGG | 0.510904 | 8.3:+4324960 | MS.gene57641:CDS |
| GTCAATTTGGGATTATAACC+CGG | 0.514638 | 8.3:+4331876 | MS.gene57641:CDS |
| ACTATAGCAGCAAGAGGGCA+AGG | 0.517048 | 8.3:+4324723 | MS.gene57641:CDS |
| CTTAAACAAAACATTACCGC+TGG | 0.517824 | 8.3:+4332889 | MS.gene57641:CDS |
| TCCAAGAGCTATTCTTGCCC+GGG | 0.517850 | 8.3:-4331894 | None:intergenic |
| AACTGGGTTTGATAATTTGG+AGG | 0.517975 | 8.3:+4333091 | MS.gene57641:CDS |
| TTTCACAGGGAAAGGAGACT+TGG | 0.527908 | 8.3:+4331796 | MS.gene57641:intron |
| GAACATGTTACTTACCCACT+TGG | 0.533058 | 8.3:-4331924 | None:intergenic |
| AAATAATCAGTCCAAGAAAG+TGG | 0.540656 | 8.3:-4324924 | None:intergenic |
| TCAATTTGGGATTATAACCC+GGG | 0.543945 | 8.3:+4331877 | MS.gene57641:CDS |
| CAAATAAAATATTGTCACCT+GGG | 0.545989 | 8.3:+4333270 | MS.gene57641:CDS |
| GATAAATATGTAGATGTTGG+TGG | 0.546726 | 8.3:+4324849 | MS.gene57641:CDS |
| TCTTCATGTGTTCTGTTTAG+GGG | 0.551746 | 8.3:-4333185 | None:intergenic |
| GAGGCTAGTGAAATGGTCAC+GGG | 0.552141 | 8.3:-4324588 | None:intergenic |
| AGAATGTCTGAATTTAATGA+AGG | 0.553370 | 8.3:+4332847 | MS.gene57641:CDS |
| TTTAATGAAGGTGTGTTCAA+GGG | 0.557994 | 8.3:+4332859 | MS.gene57641:CDS |
| GCTGCTATAGTAAAAGGGAT+AGG | 0.561618 | 8.3:-4324711 | None:intergenic |
| TTTGATAAATATGTAGATGT+TGG | 0.561969 | 8.3:+4324846 | MS.gene57641:CDS |
| GACAATCCATGACAAATGAT+GGG | 0.567061 | 8.3:+4324760 | MS.gene57641:CDS |
| AAAATATTGTCACCTGGGCA+AGG | 0.567615 | 8.3:+4333275 | MS.gene57641:CDS |
| TAGTTGAAAATGCCTTGCCC+AGG | 0.572154 | 8.3:-4333287 | None:intergenic |
| TGAGGCTAGTGAAATGGTCA+CGG | 0.586140 | 8.3:-4324589 | None:intergenic |
| AACAAGCACAACTACAACCA+TGG | 0.591747 | 8.3:+4324517 | MS.gene57641:CDS |
| TACTGATGAGCTAACACTAA+GGG | 0.595369 | 8.3:+4332768 | MS.gene57641:CDS |
| TTGCAATTCTGTAAGGATGT+CGG | 0.599370 | 8.3:+4333140 | MS.gene57641:CDS |
| CAGAACACATGAAGAATGGG+TGG | 0.600011 | 8.3:+4333193 | MS.gene57641:CDS |
| AAAGTTAATAAACTATGGCA+AGG | 0.605305 | 8.3:+4324433 | MS.gene57641:CDS |
| CTCTTGGACCTGCACCCAAG+TGG | 0.606022 | 8.3:+4331909 | MS.gene57641:CDS |
| AACATGTTACTTACCCACTT+GGG | 0.607057 | 8.3:-4331923 | None:intergenic |
| AAATATGTAGATGTTGGTGG+TGG | 0.607390 | 8.3:+4324852 | MS.gene57641:CDS |
| GGTGCATCTAGGGGTGACCA+TGG | 0.610908 | 8.3:-4324534 | None:intergenic |
| ATACTGATGAGCTAACACTA+AGG | 0.611848 | 8.3:+4332767 | MS.gene57641:CDS |
| GAGCTAACACTAAGGGCACA+AGG | 0.614915 | 8.3:+4332775 | MS.gene57641:CDS |
| GGTCACGACTAAGCATTTGG+AGG | 0.616476 | 8.3:-4324566 | None:intergenic |
| CTATAGCAGCAAGAGGGCAA+GGG | 0.617692 | 8.3:+4324724 | MS.gene57641:CDS |
| CATGACAAATGATGGGATTG+TGG | 0.622617 | 8.3:+4324767 | MS.gene57641:CDS |
| TCTTGGACCTGCACCCAAGT+GGG | 0.630916 | 8.3:+4331910 | MS.gene57641:CDS |
| TATGCCATATTGGTTTACCA+AGG | 0.647993 | 8.3:-4332456 | None:intergenic |
| AGCTAACACTAAGGGCACAA+GGG | 0.649584 | 8.3:+4332776 | MS.gene57641:CDS |
| ATAGGTCTAACCAAGGATGT+GGG | 0.663453 | 8.3:-4332810 | None:intergenic |
| TATGTGGCCAAAATCGGTTG+AGG | 0.666331 | 8.3:-4324607 | None:intergenic |
| GGAACAAATAGGTCTAACCA+AGG | 0.693806 | 8.3:-4332817 | None:intergenic |
| TTTACCAAGGAAGTTATGCG+AGG | 0.707702 | 8.3:-4332443 | None:intergenic |
| AGGCTAGTGAAATGGTCACG+GGG | 0.748023 | 8.3:-4324587 | None:intergenic |
| GTACACTATTACAAGTCCAG+CGG | 0.753165 | 8.3:-4332905 | None:intergenic |
| ATGTTGGTGGTGGACAACGT+TGG | 0.759382 | 8.3:+4324862 | MS.gene57641:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 4318375 | 4333310 | 4318375 | ID=MS.gene57641 |
| chr8.3 | mRNA | 4318375 | 4333310 | 4318375 | ID=MS.gene57641.t1;Parent=MS.gene57641 |
| chr8.3 | exon | 4318375 | 4318391 | 4318375 | ID=MS.gene57641.t1.exon1;Parent=MS.gene57641.t1 |
| chr8.3 | CDS | 4318375 | 4318391 | 4318375 | ID=cds.MS.gene57641.t1;Parent=MS.gene57641.t1 |
| chr8.3 | exon | 4324354 | 4324454 | 4324354 | ID=MS.gene57641.t1.exon2;Parent=MS.gene57641.t1 |
| chr8.3 | CDS | 4324354 | 4324454 | 4324354 | ID=cds.MS.gene57641.t1;Parent=MS.gene57641.t1 |
| chr8.3 | exon | 4324508 | 4325050 | 4324508 | ID=MS.gene57641.t1.exon3;Parent=MS.gene57641.t1 |
| chr8.3 | CDS | 4324508 | 4325050 | 4324508 | ID=cds.MS.gene57641.t1;Parent=MS.gene57641.t1 |
| chr8.3 | exon | 4331804 | 4331931 | 4331804 | ID=MS.gene57641.t1.exon4;Parent=MS.gene57641.t1 |
| chr8.3 | CDS | 4331804 | 4331931 | 4331804 | ID=cds.MS.gene57641.t1;Parent=MS.gene57641.t1 |
| chr8.3 | exon | 4332272 | 4332538 | 4332272 | ID=MS.gene57641.t1.exon5;Parent=MS.gene57641.t1 |
| chr8.3 | CDS | 4332272 | 4332538 | 4332272 | ID=cds.MS.gene57641.t1;Parent=MS.gene57641.t1 |
| chr8.3 | exon | 4332676 | 4332944 | 4332676 | ID=MS.gene57641.t1.exon6;Parent=MS.gene57641.t1 |
| chr8.3 | CDS | 4332676 | 4332944 | 4332676 | ID=cds.MS.gene57641.t1;Parent=MS.gene57641.t1 |
| chr8.3 | exon | 4333022 | 4333310 | 4333022 | ID=MS.gene57641.t1.exon7;Parent=MS.gene57641.t1 |
| chr8.3 | CDS | 4333022 | 4333310 | 4333022 | ID=cds.MS.gene57641.t1;Parent=MS.gene57641.t1 |
| Gene Sequence |
| Protein sequence |
