Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57819.t1 | XP_003620175.1 | 94.4 | 214 | 11 | 1 | 1 | 214 | 1 | 213 | 3.30E-114 | 421 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57819.t1 | P13087 | 44.2 | 215 | 106 | 5 | 6 | 213 | 9 | 216 | 1.9e-41 | 170.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57819.t1 | Q6ISX8 | 94.4 | 214 | 11 | 1 | 1 | 214 | 1 | 213 | 2.4e-114 | 421.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05227 | MS.gene57819 | 0.926779 | 2.93E-91 | -1.69E-46 |
| MS.gene05228 | MS.gene57819 | 0.817073 | 3.98E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57819.t1 | MTR_6g078140 | 94.393 | 214 | 11 | 1 | 1 | 214 | 1 | 213 | 8.10e-136 | 379 |
| MS.gene57819.t1 | MTR_6g078120 | 93.925 | 214 | 12 | 1 | 1 | 214 | 1 | 213 | 1.18e-134 | 376 |
| MS.gene57819.t1 | MTR_6g078290 | 58.605 | 215 | 85 | 4 | 1 | 214 | 1 | 212 | 5.00e-81 | 241 |
| MS.gene57819.t1 | MTR_6g078280 | 61.000 | 200 | 71 | 4 | 18 | 214 | 17 | 212 | 8.54e-81 | 240 |
| MS.gene57819.t1 | MTR_6g078250 | 56.122 | 196 | 77 | 2 | 16 | 211 | 15 | 201 | 1.32e-71 | 216 |
| MS.gene57819.t1 | MTR_6g078260 | 47.706 | 218 | 91 | 6 | 1 | 214 | 1 | 199 | 4.61e-57 | 179 |
| MS.gene57819.t1 | MTR_7g037410 | 48.663 | 187 | 84 | 4 | 29 | 214 | 27 | 202 | 1.75e-55 | 176 |
| MS.gene57819.t1 | MTR_6g078070 | 40.359 | 223 | 104 | 10 | 1 | 214 | 1 | 203 | 2.83e-40 | 137 |
| MS.gene57819.t1 | MTR_6g078100 | 40.265 | 226 | 102 | 11 | 1 | 214 | 1 | 205 | 2.85e-39 | 134 |
| MS.gene57819.t1 | MTR_3g106620 | 29.545 | 220 | 123 | 7 | 6 | 214 | 1 | 199 | 2.14e-20 | 85.5 |
| MS.gene57819.t1 | MTR_6g065460 | 30.556 | 216 | 136 | 5 | 1 | 214 | 1 | 204 | 8.82e-20 | 84.0 |
| MS.gene57819.t1 | MTR_3g106585 | 31.390 | 223 | 113 | 9 | 6 | 214 | 1 | 197 | 1.84e-18 | 80.5 |
| MS.gene57819.t1 | MTR_2g073010 | 32.589 | 224 | 115 | 11 | 1 | 214 | 1 | 198 | 1.18e-15 | 72.8 |
| MS.gene57819.t1 | MTR_8g461050 | 29.384 | 211 | 127 | 8 | 10 | 213 | 6 | 201 | 2.85e-14 | 68.9 |
| MS.gene57819.t1 | MTR_6g059650 | 30.612 | 196 | 111 | 11 | 26 | 213 | 23 | 201 | 5.49e-14 | 68.6 |
| MS.gene57819.t1 | MTR_2g072980 | 31.308 | 214 | 121 | 10 | 5 | 214 | 8 | 199 | 1.39e-13 | 67.0 |
| MS.gene57819.t1 | MTR_6g009563 | 30.233 | 215 | 124 | 9 | 6 | 214 | 9 | 203 | 1.30e-12 | 64.7 |
| MS.gene57819.t1 | MTR_6g059410 | 28.959 | 221 | 123 | 11 | 1 | 214 | 1 | 194 | 1.93e-12 | 63.9 |
| MS.gene57819.t1 | MTR_6g078040 | 47.436 | 78 | 36 | 4 | 104 | 179 | 1 | 75 | 5.58e-12 | 60.1 |
| MS.gene57819.t1 | MTR_8g059790 | 28.421 | 190 | 114 | 8 | 31 | 213 | 29 | 203 | 1.15e-11 | 62.0 |
| MS.gene57819.t1 | MTR_6g009650 | 31.220 | 205 | 115 | 9 | 6 | 205 | 9 | 192 | 1.67e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57819.t1 | AT1G17860 | 43.578 | 218 | 96 | 8 | 1 | 214 | 1 | 195 | 1.57e-48 | 157 |
| MS.gene57819.t1 | AT1G73260 | 36.000 | 200 | 110 | 6 | 21 | 214 | 18 | 205 | 1.32e-28 | 107 |
| MS.gene57819.t1 | AT1G73325 | 33.636 | 220 | 123 | 9 | 1 | 214 | 1 | 203 | 3.07e-25 | 98.6 |
Find 51 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATCTTGAACCAATTATTAA+TGG | 0.190104 | 6.1:-69188683 | None:intergenic |
| ACACGAATAACACCCTTCTT+AGG | 0.207105 | 6.1:-69188517 | None:intergenic |
| TTGAGCACACAACCACTTCT+TGG | 0.247520 | 6.1:+69188262 | MS.gene57819:CDS |
| GATGGGAAACCATTAATAAT+TGG | 0.274442 | 6.1:+69188674 | MS.gene57819:CDS |
| GGAGTGTTTGTTGATGAAAA+TGG | 0.277827 | 6.1:+69188784 | MS.gene57819:CDS |
| CACCTGTGAATGGGTATAAC+CGG | 0.336556 | 6.1:-69188364 | None:intergenic |
| AGACAATGGTTTATTACTAT+TGG | 0.339490 | 6.1:+69188631 | MS.gene57819:CDS |
| TGTTGAAATTTAACTTTAAG+AGG | 0.341900 | 6.1:-69188835 | None:intergenic |
| ACACCCTTCTTAGGGTTAAC+AGG | 0.353495 | 6.1:-69188508 | None:intergenic |
| ATTCACAGGTGTGGACCTTA+TGG | 0.370571 | 6.1:+69188376 | MS.gene57819:CDS |
| TGAAAATGGGAATAAACGTT+TGG | 0.383026 | 6.1:+69188798 | MS.gene57819:CDS |
| TGGTTCAAGATTGAGAAGTA+TGG | 0.396705 | 6.1:+69188694 | MS.gene57819:CDS |
| TGGTGGTGTTGTTGGAAATC+CGG | 0.404788 | 6.1:+69188651 | MS.gene57819:CDS |
| GAGTGTTTGTTGATGAAAAT+GGG | 0.410318 | 6.1:+69188785 | MS.gene57819:CDS |
| ATTACTATTGGTGGTGTTGT+TGG | 0.423355 | 6.1:+69188643 | MS.gene57819:CDS |
| GAACAAGTGGTTGACACATT+AGG | 0.434007 | 6.1:+69188304 | MS.gene57819:CDS |
| TATGCCTGTTAACCCTAAGA+AGG | 0.446168 | 6.1:+69188504 | MS.gene57819:CDS |
| CACGAATAACACCCTTCTTA+GGG | 0.454923 | 6.1:-69188516 | None:intergenic |
| CTACTTCTACATTTACCATA+AGG | 0.458135 | 6.1:-69188391 | None:intergenic |
| TTGAAAGACTGCACCACACT+AGG | 0.459638 | 6.1:-69188742 | None:intergenic |
| AGTCTTGCCCTAGCAAGTAT+TGG | 0.463122 | 6.1:+69188421 | MS.gene57819:CDS |
| GTCTTTCAAGCATATGTGTA+AGG | 0.466167 | 6.1:+69188756 | MS.gene57819:CDS |
| AACTTGCCCTCTTGATGTTG+TGG | 0.473079 | 6.1:+69188447 | MS.gene57819:CDS |
| CAATGGTTTATTACTATTGG+TGG | 0.475905 | 6.1:+69188634 | MS.gene57819:CDS |
| TTATTAATGGTTTCCCATCC+CGG | 0.501459 | 6.1:-69188670 | None:intergenic |
| AAGCATATGTGTAAGGATGT+TGG | 0.504444 | 6.1:+69188763 | MS.gene57819:CDS |
| ATAAGGTCCACACCTGTGAA+TGG | 0.514083 | 6.1:-69188374 | None:intergenic |
| TAAGGTCCACACCTGTGAAT+GGG | 0.519817 | 6.1:-69188373 | None:intergenic |
| TGCTAATTACTATATTATCC+CGG | 0.540548 | 6.1:+69188345 | MS.gene57819:CDS |
| GGTGGTGTTGTTGGAAATCC+GGG | 0.542963 | 6.1:+69188652 | MS.gene57819:CDS |
| GCTTCAGCTGCTCCAAGAAG+TGG | 0.551993 | 6.1:-69188274 | None:intergenic |
| TCCCGGTTATACCCATTCAC+AGG | 0.554778 | 6.1:+69188362 | MS.gene57819:CDS |
| ATGCCTGTTAACCCTAAGAA+GGG | 0.557642 | 6.1:+69188505 | MS.gene57819:CDS |
| GTGTTGTTGGAAATCCGGGA+TGG | 0.564391 | 6.1:+69188656 | MS.gene57819:CDS |
| TGTTGTTGGAAATCCGGGAT+GGG | 0.569448 | 6.1:+69188657 | MS.gene57819:CDS |
| TGAAAGACTGCACCACACTA+GGG | 0.577137 | 6.1:-69188741 | None:intergenic |
| TTAACAGGCATAAAAGTAAG+TGG | 0.579627 | 6.1:-69188493 | None:intergenic |
| TATGGTAAATGTAGAAGTAG+TGG | 0.581114 | 6.1:+69188394 | MS.gene57819:CDS |
| CAAGCTTCCACACCATGGAA+TGG | 0.584005 | 6.1:-69188588 | None:intergenic |
| TGAAGCTTCAAATGAACAAG+TGG | 0.587694 | 6.1:+69188291 | MS.gene57819:CDS |
| AAGTGGTTGTGTGCTCAAGA+AGG | 0.588756 | 6.1:-69188257 | None:intergenic |
| GTCTACACCATTCCATGGTG+TGG | 0.596900 | 6.1:+69188581 | MS.gene57819:CDS |
| ACCTGTGAATGGGTATAACC+GGG | 0.598899 | 6.1:-69188363 | None:intergenic |
| AACTTGTCTACACCATTCCA+TGG | 0.601053 | 6.1:+69188576 | MS.gene57819:CDS |
| TTAATGTATCTAAGAGACAA+TGG | 0.603832 | 6.1:+69188617 | MS.gene57819:CDS |
| TCAACAACCACAACATCAAG+AGG | 0.610607 | 6.1:-69188454 | None:intergenic |
| GTATGGTGATGATTATAAGT+TGG | 0.617624 | 6.1:+69188711 | MS.gene57819:CDS |
| TATAGTAATTAGCATCAGCT+CGG | 0.621190 | 6.1:-69188336 | None:intergenic |
| GTTATACCCATTCACAGGTG+TGG | 0.621916 | 6.1:+69188367 | MS.gene57819:CDS |
| ACGATCAAGCTTCCACACCA+TGG | 0.629046 | 6.1:-69188593 | None:intergenic |
| CAACAACCACAACATCAAGA+GGG | 0.678487 | 6.1:-69188453 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATCTTGAACCAATTATTAA+TGG | - | chr6.1:69188686-69188705 | None:intergenic | 20.0% |
| !!! | TGTTGAAATTTAACTTTAAG+AGG | - | chr6.1:69188838-69188857 | None:intergenic | 20.0% |
| ! | AGACAATGGTTTATTACTAT+TGG | + | chr6.1:69188631-69188650 | MS.gene57819:CDS | 25.0% |
| ! | TGCTAATTACTATATTATCC+CGG | + | chr6.1:69188345-69188364 | MS.gene57819:CDS | 25.0% |
| ! | TTAATGTATCTAAGAGACAA+TGG | + | chr6.1:69188617-69188636 | MS.gene57819:CDS | 25.0% |
| CAATGGTTTATTACTATTGG+TGG | + | chr6.1:69188634-69188653 | MS.gene57819:CDS | 30.0% | |
| CTACTTCTACATTTACCATA+AGG | - | chr6.1:69188394-69188413 | None:intergenic | 30.0% | |
| GATGGGAAACCATTAATAAT+TGG | + | chr6.1:69188674-69188693 | MS.gene57819:CDS | 30.0% | |
| TATAGTAATTAGCATCAGCT+CGG | - | chr6.1:69188339-69188358 | None:intergenic | 30.0% | |
| TATGGTAAATGTAGAAGTAG+TGG | + | chr6.1:69188394-69188413 | MS.gene57819:CDS | 30.0% | |
| TGAAAATGGGAATAAACGTT+TGG | + | chr6.1:69188798-69188817 | MS.gene57819:CDS | 30.0% | |
| TTAACAGGCATAAAAGTAAG+TGG | - | chr6.1:69188496-69188515 | None:intergenic | 30.0% | |
| ! | AAGTTTTTCCAATACTTGCT+AGG | - | chr6.1:69188432-69188451 | None:intergenic | 30.0% |
| ! | GAGTGTTTGTTGATGAAAAT+GGG | + | chr6.1:69188785-69188804 | MS.gene57819:CDS | 30.0% |
| ! | GTATGGTGATGATTATAAGT+TGG | + | chr6.1:69188711-69188730 | MS.gene57819:CDS | 30.0% |
| !! | AGTTTTTCCAATACTTGCTA+GGG | - | chr6.1:69188431-69188450 | None:intergenic | 30.0% |
| AAGCATATGTGTAAGGATGT+TGG | + | chr6.1:69188763-69188782 | MS.gene57819:CDS | 35.0% | |
| GTCTTTCAAGCATATGTGTA+AGG | + | chr6.1:69188756-69188775 | MS.gene57819:CDS | 35.0% | |
| TGAAGCTTCAAATGAACAAG+TGG | + | chr6.1:69188291-69188310 | MS.gene57819:CDS | 35.0% | |
| TGGTTCAAGATTGAGAAGTA+TGG | + | chr6.1:69188694-69188713 | MS.gene57819:CDS | 35.0% | |
| TTATTAATGGTTTCCCATCC+CGG | - | chr6.1:69188673-69188692 | None:intergenic | 35.0% | |
| ! | GGAGTGTTTGTTGATGAAAA+TGG | + | chr6.1:69188784-69188803 | MS.gene57819:CDS | 35.0% |
| !! | ATTACTATTGGTGGTGTTGT+TGG | + | chr6.1:69188643-69188662 | MS.gene57819:CDS | 35.0% |
| AACTTGTCTACACCATTCCA+TGG | + | chr6.1:69188576-69188595 | MS.gene57819:CDS | 40.0% | |
| ACACGAATAACACCCTTCTT+AGG | - | chr6.1:69188520-69188539 | None:intergenic | 40.0% | |
| ATGCCTGTTAACCCTAAGAA+GGG | + | chr6.1:69188505-69188524 | MS.gene57819:CDS | 40.0% | |
| CAACAACCACAACATCAAGA+GGG | - | chr6.1:69188456-69188475 | None:intergenic | 40.0% | |
| CACGAATAACACCCTTCTTA+GGG | - | chr6.1:69188519-69188538 | None:intergenic | 40.0% | |
| GAACAAGTGGTTGACACATT+AGG | + | chr6.1:69188304-69188323 | MS.gene57819:CDS | 40.0% | |
| TATGCCTGTTAACCCTAAGA+AGG | + | chr6.1:69188504-69188523 | MS.gene57819:CDS | 40.0% | |
| TCAACAACCACAACATCAAG+AGG | - | chr6.1:69188457-69188476 | None:intergenic | 40.0% | |
| TGCTCAAGAAGGCAAAAAGA+AGG | - | chr6.1:69188249-69188268 | None:intergenic | 40.0% | |
| AACTTGCCCTCTTGATGTTG+TGG | + | chr6.1:69188447-69188466 | MS.gene57819:CDS | 45.0% | |
| AAGTGGTTGTGTGCTCAAGA+AGG | - | chr6.1:69188260-69188279 | None:intergenic | 45.0% | |
| ACACCCTTCTTAGGGTTAAC+AGG | - | chr6.1:69188511-69188530 | None:intergenic | 45.0% | |
| ACCTGTGAATGGGTATAACC+GGG | - | chr6.1:69188366-69188385 | None:intergenic | 45.0% | |
| AGTCTTGCCCTAGCAAGTAT+TGG | + | chr6.1:69188421-69188440 | MS.gene57819:CDS | 45.0% | |
| ATAAGGTCCACACCTGTGAA+TGG | - | chr6.1:69188377-69188396 | None:intergenic | 45.0% | |
| ATTCACAGGTGTGGACCTTA+TGG | + | chr6.1:69188376-69188395 | MS.gene57819:CDS | 45.0% | |
| CACCTGTGAATGGGTATAAC+CGG | - | chr6.1:69188367-69188386 | None:intergenic | 45.0% | |
| GTTATACCCATTCACAGGTG+TGG | + | chr6.1:69188367-69188386 | MS.gene57819:CDS | 45.0% | |
| TAAGGTCCACACCTGTGAAT+GGG | - | chr6.1:69188376-69188395 | None:intergenic | 45.0% | |
| TGAAAGACTGCACCACACTA+GGG | - | chr6.1:69188744-69188763 | None:intergenic | 45.0% | |
| TGTTGTTGGAAATCCGGGAT+GGG | + | chr6.1:69188657-69188676 | MS.gene57819:CDS | 45.0% | |
| TTGAAAGACTGCACCACACT+AGG | - | chr6.1:69188745-69188764 | None:intergenic | 45.0% | |
| TTGAGCACACAACCACTTCT+TGG | + | chr6.1:69188262-69188281 | MS.gene57819:CDS | 45.0% | |
| !! | TGGTGGTGTTGTTGGAAATC+CGG | + | chr6.1:69188651-69188670 | MS.gene57819:CDS | 45.0% |
| ACGATCAAGCTTCCACACCA+TGG | - | chr6.1:69188596-69188615 | None:intergenic | 50.0% | |
| CAAGCTTCCACACCATGGAA+TGG | - | chr6.1:69188591-69188610 | None:intergenic | 50.0% | |
| GTCTACACCATTCCATGGTG+TGG | + | chr6.1:69188581-69188600 | MS.gene57819:CDS | 50.0% | |
| TCCCGGTTATACCCATTCAC+AGG | + | chr6.1:69188362-69188381 | MS.gene57819:CDS | 50.0% | |
| ! | GTGTTGTTGGAAATCCGGGA+TGG | + | chr6.1:69188656-69188675 | MS.gene57819:CDS | 50.0% |
| !! | GGTGGTGTTGTTGGAAATCC+GGG | + | chr6.1:69188652-69188671 | MS.gene57819:CDS | 50.0% |
| !! | GTTGGTGTTTTGCCCTAGTG+TGG | + | chr6.1:69188729-69188748 | MS.gene57819:CDS | 50.0% |
| GCTTCAGCTGCTCCAAGAAG+TGG | - | chr6.1:69188277-69188296 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 69188220 | 69188864 | 69188220 | ID=MS.gene57819 |
| chr6.1 | mRNA | 69188220 | 69188864 | 69188220 | ID=MS.gene57819.t1;Parent=MS.gene57819 |
| chr6.1 | exon | 69188220 | 69188864 | 69188220 | ID=MS.gene57819.t1.exon1;Parent=MS.gene57819.t1 |
| chr6.1 | CDS | 69188220 | 69188864 | 69188220 | ID=cds.MS.gene57819.t1;Parent=MS.gene57819.t1 |
| Gene Sequence |
| Protein sequence |
