Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57849.t1 | XP_003616475.1 | 100 | 58 | 0 | 0 | 1 | 58 | 1 | 58 | 6.10E-23 | 115.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57849.t1 | Q84JH5 | 94.8 | 58 | 3 | 0 | 1 | 58 | 1 | 58 | 6.2e-24 | 110.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57849.t1 | G7KGV6 | 100.0 | 58 | 0 | 0 | 1 | 58 | 1 | 58 | 4.4e-23 | 115.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051102 | MS.gene57849 | 0.804453 | 2.14E-49 | -1.69E-46 |
| MS.gene051642 | MS.gene57849 | 0.819204 | 1.31E-52 | -1.69E-46 |
| MS.gene059171 | MS.gene57849 | 0.809351 | 1.98E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57849.t1 | MTR_5g080770 | 100.000 | 58 | 0 | 0 | 1 | 58 | 1 | 58 | 3.54e-34 | 118 |
| MS.gene57849.t1 | MTR_3g069070 | 96.552 | 58 | 2 | 0 | 1 | 58 | 1 | 58 | 1.60e-32 | 114 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57849.t1 | AT2G39770 | 94.828 | 58 | 3 | 0 | 1 | 58 | 1 | 58 | 7.96e-32 | 112 |
| MS.gene57849.t1 | AT2G39770 | 94.828 | 58 | 3 | 0 | 1 | 58 | 1 | 58 | 7.96e-32 | 112 |
| MS.gene57849.t1 | AT2G39770 | 94.828 | 58 | 3 | 0 | 1 | 58 | 1 | 58 | 7.96e-32 | 112 |
| MS.gene57849.t1 | AT3G55590 | 93.103 | 58 | 4 | 0 | 1 | 58 | 1 | 58 | 1.77e-31 | 111 |
| MS.gene57849.t1 | AT4G30570 | 86.111 | 36 | 5 | 0 | 21 | 56 | 1 | 36 | 3.53e-15 | 67.4 |
Find 20 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACCTCTGGTTGGTAATTAA+TGG | 0.211786 | 5.2:-68523849 | None:intergenic |
| CACAATGAAGGCATTGATTC+TGG | 0.231325 | 5.2:+68523415 | None:intergenic |
| GCAAAATCAACAAGAGGCTT+AGG | 0.374674 | 5.2:-68523482 | None:intergenic |
| TGAAGGCATTGATTCTGGTT+GGG | 0.402317 | 5.2:+68523420 | MS.gene57849:CDS |
| ATTTCAGATAGAGGCTCTTA+AGG | 0.411528 | 5.2:+68523799 | MS.gene57849:intron |
| CTTTACCTGATGCAGAATCA+TGG | 0.441184 | 5.2:-68523513 | None:intergenic |
| CAAACCCATGATTCTGCATC+AGG | 0.445402 | 5.2:+68523508 | MS.gene57849:CDS |
| GAAGGCATTGATTCTGGTTG+GGG | 0.475018 | 5.2:+68523421 | MS.gene57849:CDS |
| ATGAAGGCATTGATTCTGGT+TGG | 0.494195 | 5.2:+68523419 | MS.gene57849:CDS |
| TGGAGTCACTGAAGTGGTAC+TGG | 0.502053 | 5.2:+68523826 | MS.gene57849:CDS |
| TTAGGGACACTGAGTGTCAA+TGG | 0.504104 | 5.2:-68523464 | None:intergenic |
| TTTACCTGATGCAGAATCAT+GGG | 0.506591 | 5.2:-68523512 | None:intergenic |
| CAAAATCAACAAGAGGCTTA+GGG | 0.510775 | 5.2:-68523481 | None:intergenic |
| ATAGAGGCTCTTAAGGCGAC+TGG | 0.515685 | 5.2:+68523806 | MS.gene57849:CDS |
| TGATCCTCCTTACCTCTGGT+TGG | 0.553108 | 5.2:-68523859 | None:intergenic |
| AGGCATTGATTCTGGTTGGG+GGG | 0.577286 | 5.2:+68523423 | MS.gene57849:CDS |
| AAGGCATTGATTCTGGTTGG+GGG | 0.602293 | 5.2:+68523422 | MS.gene57849:CDS |
| TTGTTAGCAAAATCAACAAG+AGG | 0.603818 | 5.2:-68523488 | None:intergenic |
| GGCGACTGGAGTCACTGAAG+TGG | 0.645491 | 5.2:+68523820 | MS.gene57849:CDS |
| GGCCATTAATTACCAACCAG+AGG | 0.667310 | 5.2:+68523847 | MS.gene57849:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAACAGATATAAAACTTAT+AGG | - | chr5.2:68523557-68523576 | None:intergenic | 15.0% |
| !! | TAACAGATATAAAACTTATA+GGG | - | chr5.2:68523556-68523575 | None:intergenic | 15.0% |
| !!! | ATTTAATTTTGCCATATTAA+GGG | - | chr5.2:68523778-68523797 | None:intergenic | 15.0% |
| !!! | GAAGTCATTTAATTTTAAAA+AGG | - | chr5.2:68523681-68523700 | None:intergenic | 15.0% |
| !! | AATTAAATGATTTCAGATAG+AGG | + | chr5.2:68523790-68523809 | MS.gene57849:intron | 20.0% |
| !!! | CATTTAATTTTGCCATATTA+AGG | - | chr5.2:68523779-68523798 | None:intergenic | 20.0% |
| !!! | TATTTCTATTTCCCTTAATA+TGG | + | chr5.2:68523764-68523783 | MS.gene57849:intron | 20.0% |
| !!! | TTATGTTCTAGAATATGTTT+GGG | + | chr5.2:68523573-68523592 | MS.gene57849:intron | 20.0% |
| ! | GTTCTACACAAATAAAAAAG+TGG | - | chr5.2:68523719-68523738 | None:intergenic | 25.0% |
| !!! | GTTATGTTCTAGAATATGTT+TGG | + | chr5.2:68523572-68523591 | MS.gene57849:intron | 25.0% |
| AATCGAAAATCTGTGTTATG+TGG | - | chr5.2:68523657-68523676 | None:intergenic | 30.0% | |
| TATGCACATAAATACATGAC+AGG | - | chr5.2:68523741-68523760 | None:intergenic | 30.0% | |
| TTGTTAGCAAAATCAACAAG+AGG | - | chr5.2:68523491-68523510 | None:intergenic | 30.0% | |
| ATTTCAGATAGAGGCTCTTA+AGG | + | chr5.2:68523799-68523818 | MS.gene57849:intron | 35.0% | |
| CAAAATCAACAAGAGGCTTA+GGG | - | chr5.2:68523484-68523503 | None:intergenic | 35.0% | |
| TGTTGAATGCTTTCTAAACC+CGG | + | chr5.2:68523607-68523626 | MS.gene57849:intron | 35.0% | |
| TTTACCTGATGCAGAATCAT+GGG | - | chr5.2:68523515-68523534 | None:intergenic | 35.0% | |
| CTTTACCTGATGCAGAATCA+TGG | - | chr5.2:68523516-68523535 | None:intergenic | 40.0% | |
| GCAAAATCAACAAGAGGCTT+AGG | - | chr5.2:68523485-68523504 | None:intergenic | 40.0% | |
| ! | AAAGCATCAGAATCAGATGC+CGG | - | chr5.2:68523629-68523648 | None:intergenic | 40.0% |
| !! | ATGAAGGCATTGATTCTGGT+TGG | + | chr5.2:68523419-68523438 | MS.gene57849:CDS | 40.0% |
| !! | TGAAGGCATTGATTCTGGTT+GGG | + | chr5.2:68523420-68523439 | MS.gene57849:CDS | 40.0% |
| AAGCATCAGAATCAGATGCC+GGG | - | chr5.2:68523628-68523647 | None:intergenic | 45.0% | |
| CAAACCCATGATTCTGCATC+AGG | + | chr5.2:68523508-68523527 | MS.gene57849:CDS | 45.0% | |
| TTAGGGACACTGAGTGTCAA+TGG | - | chr5.2:68523467-68523486 | None:intergenic | 45.0% | |
| !! | AAGGCATTGATTCTGGTTGG+GGG | + | chr5.2:68523422-68523441 | MS.gene57849:CDS | 45.0% |
| !! | GAAGGCATTGATTCTGGTTG+GGG | + | chr5.2:68523421-68523440 | MS.gene57849:CDS | 45.0% |
| ATAGAGGCTCTTAAGGCGAC+TGG | + | chr5.2:68523806-68523825 | MS.gene57849:CDS | 50.0% | |
| TGGAGTCACTGAAGTGGTAC+TGG | + | chr5.2:68523826-68523845 | MS.gene57849:CDS | 50.0% | |
| ! | TTGATTCTGGTTGGGGGGTT+TGG | + | chr5.2:68523428-68523447 | MS.gene57849:CDS | 50.0% |
| !! | AGGCATTGATTCTGGTTGGG+GGG | + | chr5.2:68523423-68523442 | MS.gene57849:CDS | 50.0% |
| ! | GGGGGTTTGGAACTAGGTTG+AGG | + | chr5.2:68523441-68523460 | MS.gene57849:CDS | 55.0% |
| ! | TGGTTGGGGGGTTTGGAACT+AGG | + | chr5.2:68523435-68523454 | MS.gene57849:CDS | 55.0% |
| GGCGACTGGAGTCACTGAAG+TGG | + | chr5.2:68523820-68523839 | MS.gene57849:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 68523419 | 68523868 | 68523419 | ID=MS.gene57849 |
| chr5.2 | mRNA | 68523419 | 68523868 | 68523419 | ID=MS.gene57849.t1;Parent=MS.gene57849 |
| chr5.2 | exon | 68523419 | 68523529 | 68523419 | ID=MS.gene57849.t1.exon1;Parent=MS.gene57849.t1 |
| chr5.2 | CDS | 68523419 | 68523529 | 68523419 | ID=cds.MS.gene57849.t1;Parent=MS.gene57849.t1 |
| chr5.2 | exon | 68523806 | 68523868 | 68523806 | ID=MS.gene57849.t1.exon2;Parent=MS.gene57849.t1 |
| chr5.2 | CDS | 68523806 | 68523868 | 68523806 | ID=cds.MS.gene57849.t1;Parent=MS.gene57849.t1 |
| Gene Sequence |
| Protein sequence |
