Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57971.t1 | XP_003609498.2 | 94.9 | 117 | 6 | 0 | 1 | 117 | 1 | 117 | 7.00E-50 | 206.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57971.t1 | G7JGN5 | 94.9 | 117 | 6 | 0 | 1 | 117 | 1 | 117 | 5.0e-50 | 206.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059029 | MS.gene57971 | 0.805887 | 1.07E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57971.t1 | MTR_4g116360 | 94.872 | 117 | 6 | 0 | 1 | 117 | 1 | 117 | 3.64e-68 | 202 |
| MS.gene57971.t1 | MTR_2g450420 | 53.191 | 94 | 36 | 4 | 18 | 111 | 17 | 102 | 2.49e-18 | 75.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATAAAGATGTAAAAAAAA+AGG | - | 32393:3410-3429 | MS.gene57971:intergenic | 10.0% |
| !! | AATAAAGATGTAAAAAAAAA+GGG | - | 32393:3409-3428 | MS.gene57971:intergenic | 10.0% |
| !! | ATAAATTATATTATAAACCT+TGG | + | 32393:3378-3397 | MS.gene57971:intron | 10.0% |
| !! | ATCATGAAAGAAATTTAATT+AGG | + | 32393:3197-3216 | MS.gene57971:intron | 15.0% |
| !! | TAATTAAATTTCTTTCATGA+TGG | - | 32393:3198-3217 | MS.gene57971:intergenic | 15.0% |
| !! | TCAAATCTACAATTGTAAAA+TGG | - | 32393:3023-3042 | MS.gene57971:intergenic | 20.0% |
| !! | TGGAAAATTATTATCTAAAG+TGG | + | 32393:2988-3007 | MS.gene57971:intron | 20.0% |
| !!! | TTTTAAAAAAATTAGGGTAG+TGG | + | 32393:2968-2987 | MS.gene57971:intron | 20.0% |
| ! | AAAAAAAAAGGGAAATTCCA+AGG | - | 32393:3398-3417 | MS.gene57971:intergenic | 25.0% |
| ! | AAAAAAGGAAGAAAAACTTG+TGG | - | 32393:2946-2965 | MS.gene57971:intergenic | 25.0% |
| ! | ATATGTCCTTGTAATTTCAA+AGG | - | 32393:3053-3072 | MS.gene57971:intergenic | 25.0% |
| ! | TTTAGTTGTAGAAATTGAAG+TGG | - | 32393:3326-3345 | MS.gene57971:intergenic | 25.0% |
| !! | ATTTACCTTGAGTTTTTGTA+AGG | - | 32393:2482-2501 | MS.gene57971:intergenic | 25.0% |
| !! | TTGCATAGTCATAAGTTTTT+GGG | - | 32393:3103-3122 | MS.gene57971:intergenic | 25.0% |
| !! | TTTGCATAGTCATAAGTTTT+TGG | - | 32393:3104-3123 | MS.gene57971:intergenic | 25.0% |
| !!! | AAATGTTTTTATTTTGCTGC+AGG | + | 32393:3274-3293 | MS.gene57971:intron | 25.0% |
| ACTAGAAATGTGAATGTGAA+TGG | + | 32393:3507-3526 | MS.gene57971:CDS | 30.0% | |
| CAGATTCATGAAGATTACTA+TGG | + | 32393:2878-2897 | MS.gene57971:intron | 30.0% | |
| CTAGAAATGTGAATGTGAAT+GGG | + | 32393:3508-3527 | MS.gene57971:CDS | 30.0% | |
| CTGCAGGAAATATAAAGAAT+AGG | + | 32393:3290-3309 | MS.gene57971:intron | 30.0% | |
| GGAAGATTAGAAATGTAACA+AGG | - | 32393:2544-2563 | MS.gene57971:intergenic | 30.0% | |
| TACAACTAAATCCTTGTCAA+AGG | + | 32393:3336-3355 | MS.gene57971:CDS | 30.0% | |
| TAGAAATGTGAATGTGAATG+GGG | + | 32393:3509-3528 | MS.gene57971:CDS | 30.0% | |
| TAGTAATCTTCATGAATCTG+TGG | - | 32393:2878-2897 | MS.gene57971:intergenic | 30.0% | |
| TATGACTATGCAAAAATGCA+AGG | + | 32393:3110-3129 | MS.gene57971:intron | 30.0% | |
| TGTGGACTTGAAAAAACTAA+TGG | - | 32393:2928-2947 | MS.gene57971:intergenic | 30.0% | |
| TTATAAACCAACCTTTGACA+AGG | - | 32393:3350-3369 | MS.gene57971:intergenic | 30.0% | |
| ! | TGCATGCTTTCACATTTTTA+TGG | + | 32393:2680-2699 | MS.gene57971:intron | 30.0% |
| !!! | AGTGTTACTTCTTTTCAACA+AGG | - | 32393:2756-2775 | MS.gene57971:intergenic | 30.0% |
| AATGACCAACAAAAATGCAC+AGG | + | 32393:2800-2819 | MS.gene57971:CDS | 35.0% | |
| ACTAAATCCTTGTCAAAGGT+TGG | + | 32393:3340-3359 | MS.gene57971:intron | 35.0% | |
| CTTCTCCTTACAAAAACTCA+AGG | + | 32393:2474-2493 | MS.gene57971:CDS | 35.0% | |
| GACAAAATTTGTCAGCTTAG+TGG | - | 32393:3081-3100 | MS.gene57971:intergenic | 35.0% | |
| GAGCAACAAAAACAACATGT+AGG | + | 32393:2612-2631 | MS.gene57971:intron | 35.0% | |
| TATTCTCTTCTCTTGGTTTC+TGG | + | 32393:3136-3155 | MS.gene57971:intron | 35.0% | |
| ! | ATTATCTAAAGTGGCGCTAA+TGG | + | 32393:2997-3016 | MS.gene57971:intron | 35.0% |
| ! | TAACTGGTTGTGTGATTTTC+TGG | - | 32393:2855-2874 | MS.gene57971:intergenic | 35.0% |
| ! | TCTAGTATTGCCATTTACTG+AGG | - | 32393:3493-3512 | MS.gene57971:intergenic | 35.0% |
| !! | AAGAAGTAACACTGATTCTG+AGG | + | 32393:2763-2782 | MS.gene57971:CDS | 35.0% |
| !! | AAGGCTTTATTCTCTTCTCT+TGG | + | 32393:3129-3148 | MS.gene57971:intron | 35.0% |
| !! | CTTCGTCCTTTGAAATTACA+AGG | + | 32393:3044-3063 | MS.gene57971:intron | 35.0% |
| !! | CTTTAGAAGCTGTTTTCTTC+AGG | - | 32393:2829-2848 | MS.gene57971:intergenic | 35.0% |
| ACAGAATGTGAAGAACACTG+AGG | + | 32393:3455-3474 | MS.gene57971:intron | 40.0% | |
| CTTCATGAATCTGTGGTAAC+TGG | - | 32393:2871-2890 | MS.gene57971:intergenic | 40.0% | |
| TATCCCTGCACACACAAAAA+AGG | - | 32393:2565-2584 | MS.gene57971:intergenic | 40.0% | |
| !! | AAGTTCACTTTTCTGACTGC+AGG | + | 32393:2715-2734 | MS.gene57971:intron | 40.0% |
| !!! | CTTCCTTTTTTGTGTGTGCA+GGG | + | 32393:2559-2578 | MS.gene57971:intron | 40.0% |
| !!! | TCTTCCTTTTTTGTGTGTGC+AGG | + | 32393:2558-2577 | MS.gene57971:intron | 40.0% |
| AGAGAGACTGCTACACAAGA+AGG | - | 32393:2441-2460 | MS.gene57971:intergenic | 45.0% | |
| GAATGTGAATGGGGAAGCAA+AGG | + | 32393:3518-3537 | MS.gene57971:CDS | 45.0% | |
| GACCTAGACGACATAGATCT+AGG | + | 32393:2900-2919 | MS.gene57971:intron | 45.0% | |
| TGCCTAGATCTATGTCGTCT+AGG | - | 32393:2905-2924 | MS.gene57971:intergenic | 45.0% | |
| TGTGAAGAACACTGAGGATG+TGG | + | 32393:3461-3480 | MS.gene57971:CDS | 45.0% | |
| ! | ACTTTTCTGACTGCAGGATG+AGG | + | 32393:2721-2740 | MS.gene57971:intron | 45.0% |
| ! | GGCTACCTGTGCATTTTTGT+TGG | - | 32393:2808-2827 | MS.gene57971:intergenic | 45.0% |
| !!! | AATTTTTTTAAAAGAAAAAA+AGG | - | 32393:2961-2980 | MS.gene57971:intergenic | 5.0% |
| !!! | TTTTTCTTTTAAAAAAATTA+GGG | + | 32393:2962-2981 | MS.gene57971:intron | 5.0% |
| !!! | TTTTTTCTTTTAAAAAAATT+AGG | + | 32393:2961-2980 | MS.gene57971:intron | 5.0% |
| GCAGGGATACGTTTGGGAAA+AGG | + | 32393:2576-2595 | MS.gene57971:intron | 50.0% | |
| GTGGCTCACACCTCAGTAAA+TGG | + | 32393:3480-3499 | MS.gene57971:CDS | 50.0% | |
| GTGTGTGCAGGGATACGTTT+GGG | + | 32393:2570-2589 | MS.gene57971:intron | 50.0% | |
| TGTGTGTGCAGGGATACGTT+TGG | + | 32393:2569-2588 | MS.gene57971:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 32393 | gene | 2432 | 3548 | 2432 | ID=MS.gene57971 |
| 32393 | mRNA | 2432 | 3548 | 2432 | ID=MS.gene57971.t1;Parent=MS.gene57971 |
| 32393 | exon | 2432 | 2495 | 2432 | ID=MS.gene57971.t1.exon1;Parent=MS.gene57971.t1 |
| 32393 | CDS | 2432 | 2495 | 2432 | ID=cds.MS.gene57971.t1;Parent=MS.gene57971.t1 |
| 32393 | exon | 2580 | 2629 | 2580 | ID=MS.gene57971.t1.exon2;Parent=MS.gene57971.t1 |
| 32393 | CDS | 2580 | 2629 | 2580 | ID=cds.MS.gene57971.t1;Parent=MS.gene57971.t1 |
| 32393 | exon | 2737 | 2821 | 2737 | ID=MS.gene57971.t1.exon3;Parent=MS.gene57971.t1 |
| 32393 | CDS | 2737 | 2821 | 2737 | ID=cds.MS.gene57971.t1;Parent=MS.gene57971.t1 |
| 32393 | exon | 3296 | 3357 | 3296 | ID=MS.gene57971.t1.exon4;Parent=MS.gene57971.t1 |
| 32393 | CDS | 3296 | 3357 | 3296 | ID=cds.MS.gene57971.t1;Parent=MS.gene57971.t1 |
| 32393 | exon | 3459 | 3548 | 3459 | ID=MS.gene57971.t1.exon5;Parent=MS.gene57971.t1 |
| 32393 | CDS | 3459 | 3548 | 3459 | ID=cds.MS.gene57971.t1;Parent=MS.gene57971.t1 |
| Gene Sequence |
| Protein sequence |
