Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57972.t1 | XP_003609498.2 | 96 | 150 | 6 | 0 | 1 | 150 | 1 | 150 | 1.30E-69 | 272.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57972.t1 | G7JGN5 | 96.0 | 150 | 6 | 0 | 1 | 150 | 1 | 150 | 9.5e-70 | 272.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059029 | MS.gene57972 | 0.801905 | 7.22E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57972.t1 | MTR_4g116360 | 96.000 | 150 | 6 | 0 | 1 | 150 | 1 | 150 | 3.92e-95 | 271 |
| MS.gene57972.t1 | MTR_2g450420 | 46.809 | 141 | 63 | 5 | 18 | 150 | 17 | 153 | 1.01e-29 | 105 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 25 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGGCTCACACCTCAGTAAA+TGG | 0.325930 | 8.1:+10573836 | MS.gene57972:CDS |
| TAGACTCAATTAATTGTGTA+TGG | 0.388334 | 8.1:-10573993 | None:intergenic |
| CTGCAGGAAATATAAAGAAT+AGG | 0.441652 | 8.1:+10573646 | MS.gene57972:intron |
| CTAGAAATGTGAATGTGAAT+GGG | 0.472633 | 8.1:+10573864 | MS.gene57972:CDS |
| GTGTGTGCAGGGATACGTTT+GGG | 0.472885 | 8.1:+10572927 | MS.gene57972:intron |
| GTTATGTCTACAAGGTCCTC+AGG | 0.481183 | 8.1:-10573935 | None:intergenic |
| ACTAGAAATGTGAATGTGAA+TGG | 0.484726 | 8.1:+10573863 | MS.gene57972:CDS |
| GCAGGGATACGTTTGGGAAA+AGG | 0.491954 | 8.1:+10572933 | MS.gene57972:intron |
| TGTGTGTGCAGGGATACGTT+TGG | 0.497727 | 8.1:+10572926 | MS.gene57972:intron |
| TACAACTAAATCCTTGTCAA+AGG | 0.499214 | 8.1:+10573692 | MS.gene57972:CDS |
| CCATCTCAGTTATGTCTACA+AGG | 0.517334 | 8.1:-10573943 | None:intergenic |
| CCTTGTAGACATAACTGAGA+TGG | 0.558484 | 8.1:+10573943 | MS.gene57972:CDS |
| TTTAGTTGTAGAAATTGAAG+TGG | 0.566395 | 8.1:-10573679 | None:intergenic |
| TGTGAAGAACACTGAGGATG+TGG | 0.567568 | 8.1:+10573817 | MS.gene57972:CDS |
| AGAGAGACTGCTACACAAGA+AGG | 0.574354 | 8.1:-10572795 | None:intergenic |
| AGACTCAATTAATTGTGTAT+GGG | 0.578163 | 8.1:-10573992 | None:intergenic |
| AAGAAGTAACACTGATTCTG+AGG | 0.585024 | 8.1:+10573120 | MS.gene57972:CDS |
| AGATGGATTATTCACCAGCT+AGG | 0.588549 | 8.1:+10573960 | MS.gene57972:CDS |
| TCTAGTATTGCCATTTACTG+AGG | 0.596383 | 8.1:-10573846 | None:intergenic |
| TTATAAACCAACCTTTGACA+AGG | 0.606206 | 8.1:-10573703 | None:intergenic |
| TTATGTCTACAAGGTCCTCA+GGG | 0.619907 | 8.1:-10573934 | None:intergenic |
| TAGAAATGTGAATGTGAATG+GGG | 0.652177 | 8.1:+10573865 | MS.gene57972:CDS |
| GAATGTGAATGGGGAAGCAA+AGG | 0.660105 | 8.1:+10573874 | MS.gene57972:CDS |
| TACTTCTAATTATCTCCCTG+AGG | 0.676211 | 8.1:+10573919 | MS.gene57972:CDS |
| ACAGAATGTGAAGAACACTG+AGG | 0.882326 | 8.1:+10573811 | MS.gene57972:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATAAAGATGTAAAAAAAA+AGG | - | chr8.1:10573766-10573785 | None:intergenic | 10.0% |
| !! | AATAAAGATGTAAAAAAAAA+GGG | - | chr8.1:10573765-10573784 | None:intergenic | 10.0% |
| !! | ATAAATTATATTATAAACCT+TGG | + | chr8.1:10573734-10573753 | MS.gene57972:intron | 10.0% |
| !! | ATCATGAAAGAAATTTAATT+AGG | + | chr8.1:10573553-10573572 | MS.gene57972:intron | 15.0% |
| !! | TAATTAAATTTCTTTCATGA+TGG | - | chr8.1:10573554-10573573 | None:intergenic | 15.0% |
| !! | TCAAATCTACAATTGTAAAA+TGG | - | chr8.1:10573380-10573399 | None:intergenic | 20.0% |
| !! | TGGAAAATTATTATCTAAAG+TGG | + | chr8.1:10573345-10573364 | MS.gene57972:intron | 20.0% |
| !!! | TTTTAAAAAAATTAGGGTAG+TGG | + | chr8.1:10573325-10573344 | MS.gene57972:intron | 20.0% |
| ! | AAAAAAAAAGGGAAATTCCA+AGG | - | chr8.1:10573754-10573773 | None:intergenic | 25.0% |
| ! | AAAAAAGGAAGAAAAACTTG+TGG | - | chr8.1:10573303-10573322 | None:intergenic | 25.0% |
| ! | AATATGTCCTGTAATTTCAA+AGG | - | chr8.1:10573410-10573429 | None:intergenic | 25.0% |
| ! | TTTAGTTGTAGAAATTGAAG+TGG | - | chr8.1:10573682-10573701 | None:intergenic | 25.0% |
| !! | ATTTACCTTGAGTTTTTGTA+AGG | - | chr8.1:10572839-10572858 | None:intergenic | 25.0% |
| !! | TTGCATAGTCATAAGTTTTT+GGG | - | chr8.1:10573459-10573478 | None:intergenic | 25.0% |
| !! | TTTGCATAGTCATAAGTTTT+TGG | - | chr8.1:10573460-10573479 | None:intergenic | 25.0% |
| !!! | AAATGTTTTTATTTTGCTGC+AGG | + | chr8.1:10573630-10573649 | MS.gene57972:intron | 25.0% |
| ACTAGAAATGTGAATGTGAA+TGG | + | chr8.1:10573863-10573882 | MS.gene57972:CDS | 30.0% | |
| CAGATTCATGAAGATTACTA+TGG | + | chr8.1:10573235-10573254 | MS.gene57972:intron | 30.0% | |
| CTAGAAATGTGAATGTGAAT+GGG | + | chr8.1:10573864-10573883 | MS.gene57972:CDS | 30.0% | |
| CTGCAGGAAATATAAAGAAT+AGG | + | chr8.1:10573646-10573665 | MS.gene57972:intron | 30.0% | |
| GGAAGATTAGAAATGTAACA+AGG | - | chr8.1:10572901-10572920 | None:intergenic | 30.0% | |
| TACAACTAAATCCTTGTCAA+AGG | + | chr8.1:10573692-10573711 | MS.gene57972:CDS | 30.0% | |
| TAGAAATGTGAATGTGAATG+GGG | + | chr8.1:10573865-10573884 | MS.gene57972:CDS | 30.0% | |
| TAGTAATCTTCATGAATCTG+TGG | - | chr8.1:10573235-10573254 | None:intergenic | 30.0% | |
| TATGACTATGCAAAAATGCA+AGG | + | chr8.1:10573466-10573485 | MS.gene57972:intron | 30.0% | |
| TGTGGACTTGAAAAAACTAA+TGG | - | chr8.1:10573285-10573304 | None:intergenic | 30.0% | |
| TTATAAACCAACCTTTGACA+AGG | - | chr8.1:10573706-10573725 | None:intergenic | 30.0% | |
| ! | TGCATGCTTTCACATTTTTA+TGG | + | chr8.1:10573037-10573056 | MS.gene57972:intron | 30.0% |
| !!! | AGTGTTACTTCTTTTCAACA+AGG | - | chr8.1:10573113-10573132 | None:intergenic | 30.0% |
| AATGACCAACAAAAATGCAC+AGG | + | chr8.1:10573157-10573176 | MS.gene57972:CDS | 35.0% | |
| ACTAAATCCTTGTCAAAGGT+TGG | + | chr8.1:10573696-10573715 | MS.gene57972:intron | 35.0% | |
| CTTCTCCTTACAAAAACTCA+AGG | + | chr8.1:10572831-10572850 | MS.gene57972:CDS | 35.0% | |
| GACAAAATTTGTCAGCTTAG+TGG | - | chr8.1:10573437-10573456 | None:intergenic | 35.0% | |
| GAGCAACAAAAACAACATGT+AGG | + | chr8.1:10572969-10572988 | MS.gene57972:intron | 35.0% | |
| TACTTCTAATTATCTCCCTG+AGG | + | chr8.1:10573919-10573938 | MS.gene57972:CDS | 35.0% | |
| TATTCTCTTCTCTTGGTTTC+TGG | + | chr8.1:10573492-10573511 | MS.gene57972:intron | 35.0% | |
| ! | ATTATCTAAAGTGGCGCTAA+TGG | + | chr8.1:10573354-10573373 | MS.gene57972:intron | 35.0% |
| ! | TAACTGGTTGTGTGATTTTC+TGG | - | chr8.1:10573212-10573231 | None:intergenic | 35.0% |
| ! | TCTAGTATTGCCATTTACTG+AGG | - | chr8.1:10573849-10573868 | None:intergenic | 35.0% |
| !! | AAGAAGTAACACTGATTCTG+AGG | + | chr8.1:10573120-10573139 | MS.gene57972:CDS | 35.0% |
| !! | AAGGCTTTATTCTCTTCTCT+TGG | + | chr8.1:10573485-10573504 | MS.gene57972:intron | 35.0% |
| !! | ACTTCGTCCTTTGAAATTAC+AGG | + | chr8.1:10573400-10573419 | MS.gene57972:intron | 35.0% |
| !! | CTTTAGAAGCTGTTTTCTTC+AGG | - | chr8.1:10573186-10573205 | None:intergenic | 35.0% |
| ACAGAATGTGAAGAACACTG+AGG | + | chr8.1:10573811-10573830 | MS.gene57972:intron | 40.0% | |
| CCATCTCAGTTATGTCTACA+AGG | - | chr8.1:10573946-10573965 | None:intergenic | 40.0% | |
| CCTTGTAGACATAACTGAGA+TGG | + | chr8.1:10573943-10573962 | MS.gene57972:CDS | 40.0% | |
| CTTCATGAATCTGTGGTAAC+TGG | - | chr8.1:10573228-10573247 | None:intergenic | 40.0% | |
| TATCCCTGCACACACAAAAA+AGG | - | chr8.1:10572922-10572941 | None:intergenic | 40.0% | |
| TTATGTCTACAAGGTCCTCA+GGG | - | chr8.1:10573937-10573956 | None:intergenic | 40.0% | |
| !! | AAGTTCACTTTTCTGACTGC+AGG | + | chr8.1:10573072-10573091 | MS.gene57972:intron | 40.0% |
| !! | AGATGGATTATTCACCAGCT+AGG | + | chr8.1:10573960-10573979 | MS.gene57972:CDS | 40.0% |
| !!! | CTTCCTTTTTTGTGTGTGCA+GGG | + | chr8.1:10572916-10572935 | MS.gene57972:intron | 40.0% |
| !!! | TCTTCCTTTTTTGTGTGTGC+AGG | + | chr8.1:10572915-10572934 | MS.gene57972:intron | 40.0% |
| AGAGAGACTGCTACACAAGA+AGG | - | chr8.1:10572798-10572817 | None:intergenic | 45.0% | |
| GAATGTGAATGGGGAAGCAA+AGG | + | chr8.1:10573874-10573893 | MS.gene57972:CDS | 45.0% | |
| GACCTAGACGACATAGATCT+AGG | + | chr8.1:10573257-10573276 | MS.gene57972:intron | 45.0% | |
| GTTATGTCTACAAGGTCCTC+AGG | - | chr8.1:10573938-10573957 | None:intergenic | 45.0% | |
| TGCCTAGATCTATGTCGTCT+AGG | - | chr8.1:10573262-10573281 | None:intergenic | 45.0% | |
| TGTGAAGAACACTGAGGATG+TGG | + | chr8.1:10573817-10573836 | MS.gene57972:CDS | 45.0% | |
| ! | ACTTTTCTGACTGCAGGATG+AGG | + | chr8.1:10573078-10573097 | MS.gene57972:intron | 45.0% |
| ! | GGCTACCTGTGCATTTTTGT+TGG | - | chr8.1:10573165-10573184 | None:intergenic | 45.0% |
| !! | ATGGGAGACTTTTTCCTAGC+TGG | - | chr8.1:10573977-10573996 | None:intergenic | 45.0% |
| !!! | AATTTTTTTAAAAGAAAAAA+AGG | - | chr8.1:10573318-10573337 | None:intergenic | 5.0% |
| !!! | TTTTTCTTTTAAAAAAATTA+GGG | + | chr8.1:10573319-10573338 | MS.gene57972:intron | 5.0% |
| !!! | TTTTTTCTTTTAAAAAAATT+AGG | + | chr8.1:10573318-10573337 | MS.gene57972:intron | 5.0% |
| GCAGGGATACGTTTGGGAAA+AGG | + | chr8.1:10572933-10572952 | MS.gene57972:intron | 50.0% | |
| GTGGCTCACACCTCAGTAAA+TGG | + | chr8.1:10573836-10573855 | MS.gene57972:CDS | 50.0% | |
| GTGTGTGCAGGGATACGTTT+GGG | + | chr8.1:10572927-10572946 | MS.gene57972:intron | 50.0% | |
| TGTGTGTGCAGGGATACGTT+TGG | + | chr8.1:10572926-10572945 | MS.gene57972:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 10572789 | 10574006 | 10572789 | ID=MS.gene57972 |
| chr8.1 | mRNA | 10572789 | 10574006 | 10572789 | ID=MS.gene57972.t1;Parent=MS.gene57972 |
| chr8.1 | exon | 10572789 | 10572852 | 10572789 | ID=MS.gene57972.t1.exon1;Parent=MS.gene57972.t1 |
| chr8.1 | CDS | 10572789 | 10572852 | 10572789 | ID=cds.MS.gene57972.t1;Parent=MS.gene57972.t1 |
| chr8.1 | exon | 10572937 | 10572986 | 10572937 | ID=MS.gene57972.t1.exon2;Parent=MS.gene57972.t1 |
| chr8.1 | CDS | 10572937 | 10572986 | 10572937 | ID=cds.MS.gene57972.t1;Parent=MS.gene57972.t1 |
| chr8.1 | exon | 10573094 | 10573178 | 10573094 | ID=MS.gene57972.t1.exon3;Parent=MS.gene57972.t1 |
| chr8.1 | CDS | 10573094 | 10573178 | 10573094 | ID=cds.MS.gene57972.t1;Parent=MS.gene57972.t1 |
| chr8.1 | exon | 10573652 | 10573713 | 10573652 | ID=MS.gene57972.t1.exon4;Parent=MS.gene57972.t1 |
| chr8.1 | CDS | 10573652 | 10573713 | 10573652 | ID=cds.MS.gene57972.t1;Parent=MS.gene57972.t1 |
| chr8.1 | exon | 10573815 | 10574006 | 10573815 | ID=MS.gene57972.t1.exon5;Parent=MS.gene57972.t1 |
| chr8.1 | CDS | 10573815 | 10574006 | 10573815 | ID=cds.MS.gene57972.t1;Parent=MS.gene57972.t1 |
| Gene Sequence |
| Protein sequence |
