AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene58147

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58147.t1 MTR_8g092210 99.429 175 1 0 1 175 38 212 2.69e-130 363
MS.gene58147.t1 MTR_6g043220 45.402 174 72 5 1 167 38 195 5.38e-40 134
MS.gene58147.t1 MTR_1g098250 45.614 171 88 5 1 167 38 207 1.34e-37 128
MS.gene58147.t1 MTR_6g043220 41.772 158 69 5 17 167 49 190 1.13e-29 107
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58147.t1 AT4G09830 48.588 177 58 6 1 167 38 191 5.65e-37 126
MS.gene58147.t1 AT5G64780 39.568 139 76 4 1 137 38 170 2.29e-23 91.3

Find 49 sgRNAs with CRISPR-Local

Find 191 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGATATAGTCTCTCACTTGC+AGG 0.320186 4.2:-9935933 MS.gene58147:intron
ACGAATTTGAATCTCTGTTC+TGG 0.321939 4.2:+9933687 None:intergenic
CTGCCCTGTGCTAAATGATA+TGG 0.346225 4.2:+9933779 None:intergenic
GGCTGAAGGCTGCGACGAAT+GGG 0.351898 4.2:+9933800 None:intergenic
AGTTAGTTTGAATTGCTGTT+TGG 0.364182 4.2:+9933936 None:intergenic
TAACTATGATCAAATCAGTA+TGG 0.368060 4.2:+9933611 None:intergenic
GTTAGTTTGAATTGCTGTTT+GGG 0.374257 4.2:+9933937 None:intergenic
GCATTTGGAAGTACTGTTGC+AGG 0.382751 4.2:-9933890 MS.gene58147:CDS
ATTGACTGCCTTGGAGAAAC+TGG 0.385558 4.2:+9933971 None:intergenic
ATACACCATTTCATTGTTAC+GGG 0.393219 4.2:+9933718 None:intergenic
TCCATACCGTCCAATGCATT+TGG 0.397895 4.2:-9933905 MS.gene58147:CDS
GCAGGACAGGGGATACGAAC+TGG 0.401130 4.2:-9933872 MS.gene58147:CDS
GATACACCATTTCATTGTTA+CGG 0.418071 4.2:+9933717 None:intergenic
TGGCTGAAGGCTGCGACGAA+TGG 0.418206 4.2:+9933799 None:intergenic
TCGAGTAATGTCATGTCATC+GGG 0.423559 4.2:-9933749 MS.gene58147:CDS
TGGAAGTACTGTTGCAGGAC+AGG 0.432969 4.2:-9933885 MS.gene58147:CDS
ATGTAGAATGAGCTTGAATA+TGG 0.441794 4.2:-9934007 MS.gene58147:intron
CGGAACCCGTAACAATGAAA+TGG 0.453370 4.2:-9933723 MS.gene58147:CDS
TTCTGTTGAATTGACTGCCT+TGG 0.458544 4.2:+9933962 None:intergenic
TACTGAGTTCTTTACTTGAT+CGG 0.479541 4.2:+9933844 None:intergenic
TACAAGAGCGTGGCGTTCAC+CGG 0.488524 4.2:+9936378 None:intergenic
TCCAAATGCATTGGACGGTA+TGG 0.490213 4.2:+9933904 None:intergenic
GTGGCGTTCACCGGCTTGAA+AGG 0.497863 4.2:+9936387 None:intergenic
GGAAGTACTGTTGCAGGACA+GGG 0.507937 4.2:-9933884 MS.gene58147:CDS
GTCCATGCAATCACTGGAGT+TGG 0.512023 4.2:+9933664 None:intergenic
GAATCTCTGTTCTGGCCACT+AGG 0.523458 4.2:+9933695 None:intergenic
TTCGAGTAATGTCATGTCAT+CGG 0.525561 4.2:-9933750 MS.gene58147:CDS
TGCATTGGACGGTATGGATG+CGG 0.534195 4.2:+9933910 None:intergenic
GTCCAACTCCAGTGATTGCA+TGG 0.554317 4.2:-9933666 MS.gene58147:CDS
CAGCCATATCATTTAGCACA+GGG 0.558095 4.2:-9933782 MS.gene58147:CDS
ATGCATGTCCATGCAATCAC+TGG 0.561386 4.2:+9933658 None:intergenic
CAGTATGGAAAATCATGACC+AGG 0.574411 4.2:+9933626 None:intergenic
AACAGTACTTCCAAATGCAT+TGG 0.577353 4.2:+9933895 None:intergenic
TCAGCCATATCATTTAGCAC+AGG 0.580324 4.2:-9933783 MS.gene58147:CDS
GACATGCATGCAGACAGTCC+TGG 0.588311 4.2:-9933644 MS.gene58147:CDS
CAGGACAGGGGATACGAACT+GGG 0.588579 4.2:-9933871 MS.gene58147:CDS
AATGTCATGTCATCGGGGAA+CGG 0.595236 4.2:-9933743 MS.gene58147:CDS
ATTCTTAGACAACAACAAGA+AGG 0.606741 4.2:-9935974 MS.gene58147:CDS
TGCAGGAGAAAATGTATCAG+TGG 0.615078 4.2:-9935997 MS.gene58147:intron
TGAGCTTGAATATGGAGCAG+AGG 0.628224 4.2:-9933999 MS.gene58147:CDS
GCTGAAGGCTGCGACGAATG+GGG 0.632700 4.2:+9933801 None:intergenic
AGGAATCACCAGTTTCTCCA+AGG 0.640475 4.2:-9933979 MS.gene58147:CDS
GAAGTACTGTTGCAGGACAG+GGG 0.643448 4.2:-9933883 MS.gene58147:CDS
GTGCTAAATGATATGGCTGA+AGG 0.646201 4.2:+9933786 None:intergenic
AATGAAATGGTGTATCCTAG+TGG 0.663062 4.2:-9933710 MS.gene58147:CDS
GTACTTCCAAATGCATTGGA+CGG 0.665522 4.2:+9933899 None:intergenic
GAAGGAAGCATACAAGAGCG+TGG 0.686735 4.2:+9936368 None:intergenic
GATGACATGACATTACTCGA+AGG 0.723139 4.2:+9933752 None:intergenic
CGAGTAATGTCATGTCATCG+GGG 0.731834 4.2:-9933748 MS.gene58147:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAATTAAAAGAAAAGAA+TGG + chr4.2:9933770-9933789 None:intergenic 10.0%
!! AATAATTTGAAAATAAGAAA+GGG + chr4.2:9933708-9933727 None:intergenic 10.0%
!! AATATTATAAACAAAATTAG+GGG + chr4.2:9933940-9933959 None:intergenic 10.0%
!! CAAATATTATAAACAAAATT+AGG + chr4.2:9933942-9933961 None:intergenic 10.0%
!! TAATAATTTGAAAATAAGAA+AGG + chr4.2:9933709-9933728 None:intergenic 10.0%
!! ATAATTTGAAAATAAGAAAG+GGG + chr4.2:9933707-9933726 None:intergenic 15.0%
!! GTATATTAAAAGAATAATCT+TGG - chr4.2:9935167-9935186 MS.gene58147:intron 15.0%
!! TGATTTAATATAAAATGCTT+TGG - chr4.2:9933997-9934016 MS.gene58147:CDS 15.0%
!!! AATAAGATATTTTATAACAC+AGG - chr4.2:9934527-9934546 MS.gene58147:intron 15.0%
!!! ATTTTCATTATGAGTTTTAT+AGG - chr4.2:9935506-9935525 MS.gene58147:intron 15.0%
!!! TAAGCTTATATAATTTTATG+AGG + chr4.2:9935283-9935302 None:intergenic 15.0%
!! ACTAAATTGTTTATTCAAAC+AGG + chr4.2:9934211-9934230 None:intergenic 20.0%
!! CTAAATTGTTTATTCAAACA+GGG + chr4.2:9934210-9934229 None:intergenic 20.0%
!! TAATATTTGATGTTACTCAA+TGG - chr4.2:9933953-9933972 MS.gene58147:CDS 20.0%
!!! AGTTAAATGGATAAACTTTT+CGG + chr4.2:9935681-9935700 None:intergenic 20.0%
!!! CACTCAAAATCATTTTTTTA+CGG + chr4.2:9934249-9934268 None:intergenic 20.0%
! AAATAATACAGACAGGAAAA+TGG + chr4.2:9935006-9935025 None:intergenic 25.0%
! ATTCAATGGAAAAACTACTT+AGG - chr4.2:9934183-9934202 MS.gene58147:intron 25.0%
! CAAAATCAATTATACTCTAG+AGG - chr4.2:9934389-9934408 MS.gene58147:intron 25.0%
! CATAAGTGCTTATACTATAA+GGG + chr4.2:9934507-9934526 None:intergenic 25.0%
! GTTGTTAGTTAAAATACTCA+TGG - chr4.2:9935609-9935628 MS.gene58147:intron 25.0%
! TAATAGATTCACAATAAGTC+CGG - chr4.2:9935739-9935758 MS.gene58147:intron 25.0%
! TAGTAAAGTCACAGATTAAT+GGG + chr4.2:9933877-9933896 None:intergenic 25.0%
! TATTTGATATCTTGATGTGA+AGG - chr4.2:9934279-9934298 MS.gene58147:intron 25.0%
! TCATAAGTGCTTATACTATA+AGG + chr4.2:9934508-9934527 None:intergenic 25.0%
! TCCTTACGATTTATAAGTTA+AGG + chr4.2:9935404-9935423 None:intergenic 25.0%
! TTAGTAAAGTCACAGATTAA+TGG + chr4.2:9933878-9933897 None:intergenic 25.0%
! TTATACAATGACTAACATTG+TGG + chr4.2:9935722-9935741 None:intergenic 25.0%
! TTATTTATTGCAGAAGAGTT+AGG - chr4.2:9935020-9935039 MS.gene58147:intron 25.0%
! TTCAATGGAAAAACTACTTA+GGG - chr4.2:9934184-9934203 MS.gene58147:intron 25.0%
! TTTATCCATTTAACTCTAAG+AGG - chr4.2:9935686-9935705 MS.gene58147:intron 25.0%
! TTTATTCTCTATGCATTGTT+GGG - chr4.2:9934127-9934146 MS.gene58147:intron 25.0%
!! CTTCAAGTAGAAACAATTTT+GGG - chr4.2:9934364-9934383 MS.gene58147:intron 25.0%
!! TTCAAGTAGAAACAATTTTG+GGG - chr4.2:9934365-9934384 MS.gene58147:intron 25.0%
!! TTTAGAAGCTAATTCAAGTT+GGG - chr4.2:9935378-9935397 MS.gene58147:intron 25.0%
!! TTTTATTCTCTATGCATTGT+TGG - chr4.2:9934126-9934145 MS.gene58147:intron 25.0%
!! TTTTTCCATTGAATGAGTAA+TGG + chr4.2:9934177-9934196 None:intergenic 25.0%
!!! ATCTTTAAGCTTTTGGTTTT+TGG - chr4.2:9934816-9934835 MS.gene58147:intron 25.0%
AAACTATCCTGAAAAACTCA+TGG - chr4.2:9934598-9934617 MS.gene58147:intron 30.0%
AACTGTAGGACATAATATGT+GGG + chr4.2:9935912-9935931 None:intergenic 30.0%
ACACACCTATAAAACTCATA+AGG + chr4.2:9935430-9935449 None:intergenic 30.0%
ACACATACTGATAATAAGCA+AGG + chr4.2:9935655-9935674 None:intergenic 30.0%
ACATAATATGTGGGAAACTA+CGG + chr4.2:9935903-9935922 None:intergenic 30.0%
AGAGTTGAATTTGACATGTT+TGG + chr4.2:9934417-9934436 None:intergenic 30.0%
AGCATTAGAGCAAAATCTTA+TGG + chr4.2:9935804-9935823 None:intergenic 30.0%
ATACACCATTTCATTGTTAC+GGG + chr4.2:9936316-9936335 None:intergenic 30.0%
ATCAACCTCTTAGAGTTAAA+TGG + chr4.2:9935694-9935713 None:intergenic 30.0%
ATGTAGAATGAGCTTGAATA+TGG - chr4.2:9936024-9936043 MS.gene58147:intron 30.0%
ATTCTTAGACAACAACAAGA+AGG - chr4.2:9934057-9934076 MS.gene58147:intron 30.0%
CTGCAATAAATAATACAGAC+AGG + chr4.2:9935013-9935032 None:intergenic 30.0%
GATACACCATTTCATTGTTA+CGG + chr4.2:9936317-9936336 None:intergenic 30.0%
GCCTTAACTTATAAATCGTA+AGG - chr4.2:9935400-9935419 MS.gene58147:intron 30.0%
GCTACAAATAAGTGTATGTT+TGG + chr4.2:9934479-9934498 None:intergenic 30.0%
TAAAAGAAGAAGAAGAAGGA+AGG + chr4.2:9933684-9933703 None:intergenic 30.0%
TAAAATGCAAGAACGAAATC+TGG + chr4.2:9933840-9933859 None:intergenic 30.0%
TAACTGTAGGACATAATATG+TGG + chr4.2:9935913-9935932 None:intergenic 30.0%
TAAGCATTTGTGACATTGTT+CGG + chr4.2:9934678-9934697 None:intergenic 30.0%
TACTGAGTTCTTTACTTGAT+CGG + chr4.2:9936190-9936209 None:intergenic 30.0%
TATAGTTCAACCATCAAGAT+CGG + chr4.2:9935837-9935856 None:intergenic 30.0%
TATCATTGCCTATAGCTTAA+AGG - chr4.2:9934745-9934764 MS.gene58147:intron 30.0%
TGTGCAATAATGTTAACTGT+AGG + chr4.2:9935926-9935945 None:intergenic 30.0%
TTATAAACAAAATTAGGGGC+TGG + chr4.2:9933936-9933955 None:intergenic 30.0%
! AGATTTCGTTCTTGCATTTT+AGG - chr4.2:9933839-9933858 MS.gene58147:CDS 30.0%
! AGTGTGAAACTGTTATTTAG+TGG - chr4.2:9934788-9934807 MS.gene58147:intron 30.0%
! AGTTAGTTTGAATTGCTGTT+TGG + chr4.2:9936098-9936117 None:intergenic 30.0%
! CCTTTTATTGAATTTTGCCT+GGG - chr4.2:9935762-9935781 MS.gene58147:intron 30.0%
! CTTATTTCCATGAGTTTTTC+AGG + chr4.2:9934608-9934627 None:intergenic 30.0%
! CTTTAGAAGCTAATTCAAGT+TGG - chr4.2:9935377-9935396 MS.gene58147:intron 30.0%
! GATTTCGTTCTTGCATTTTA+GGG - chr4.2:9933840-9933859 MS.gene58147:CDS 30.0%
! GCTTCAAGTAGAAACAATTT+TGG - chr4.2:9934363-9934382 MS.gene58147:intron 30.0%
! GGACATTATCTTTAAGCTTT+TGG - chr4.2:9934809-9934828 MS.gene58147:intron 30.0%
! GGTTTATCAACCTTTTACTT+GGG + chr4.2:9934900-9934919 None:intergenic 30.0%
! TCAAGTAGAAACAATTTTGG+GGG - chr4.2:9934366-9934385 MS.gene58147:intron 30.0%
!! GAAGTCCTTATGAGTTTTAT+AGG - chr4.2:9935422-9935441 MS.gene58147:intron 30.0%
!! GTTAGTTTGAATTGCTGTTT+GGG + chr4.2:9936097-9936116 None:intergenic 30.0%
!!! TTTTGGTGTTAGATTAGTCA+TGG - chr4.2:9934959-9934978 MS.gene58147:intron 30.0%
AACAGTACTTCCAAATGCAT+TGG + chr4.2:9936139-9936158 None:intergenic 35.0%
AAGCATTTGTGACATTGTTC+GGG + chr4.2:9934677-9934696 None:intergenic 35.0%
AATGAAATGGTGTATCCTAG+TGG - chr4.2:9936321-9936340 MS.gene58147:intron 35.0%
ACGAATTTGAATCTCTGTTC+TGG + chr4.2:9936347-9936366 None:intergenic 35.0%
AGTTTCACACTCAAAGCAAT+CGG + chr4.2:9934779-9934798 None:intergenic 35.0%
CAAAGTGACCTTTAAGCTAT+AGG + chr4.2:9934756-9934775 None:intergenic 35.0%
CCAAAGGTTATATCTACATC+CGG + chr4.2:9935992-9936011 None:intergenic 35.0%
CCCAGGCAAAATTCAATAAA+AGG + chr4.2:9935765-9935784 None:intergenic 35.0%
CTGAACCATTACTCATTCAA+TGG - chr4.2:9934169-9934188 MS.gene58147:intron 35.0%
GGCAAAATTCAATAAAAGGC+CGG + chr4.2:9935761-9935780 None:intergenic 35.0%
GGCACAAGTGATAGATATTT+GGG + chr4.2:9934921-9934940 None:intergenic 35.0%
TATATCTACATCCGGATATC+AGG + chr4.2:9935984-9936003 None:intergenic 35.0%
TGGCACAAGTGATAGATATT+TGG + chr4.2:9934922-9934941 None:intergenic 35.0%
TTCGAGTAATGTCATGTCAT+CGG - chr4.2:9936281-9936300 MS.gene58147:intron 35.0%
! ACTCGTTTGAGTTTATCTAC+TGG + chr4.2:9934703-9934722 None:intergenic 35.0%
! ATGCATTGTTGGGTTCTTTT+TGG - chr4.2:9934137-9934156 MS.gene58147:intron 35.0%
! GCCTTTTATTGAATTTTGCC+TGG - chr4.2:9935761-9935780 MS.gene58147:intron 35.0%
! GGAGGCAAAATTGATTCAAT+AGG + chr4.2:9934443-9934462 None:intergenic 35.0%
! GGGTTTATCAACCTTTTACT+TGG + chr4.2:9934901-9934920 None:intergenic 35.0%
! GTTCTAACGTAAGTTGAAAC+TGG - chr4.2:9935215-9935234 MS.gene58147:intron 35.0%
! TGATACATTTTCTCCTGCAA+TGG + chr4.2:9934033-9934052 None:intergenic 35.0%
! TGCATTGTTGGGTTCTTTTT+GGG - chr4.2:9934138-9934157 MS.gene58147:intron 35.0%
! TGGTCTAGCTTCTAACTAAT+AGG - chr4.2:9935098-9935117 MS.gene58147:intron 35.0%
! TTATTGAATTTTGCCTGGGT+GGG - chr4.2:9935766-9935785 MS.gene58147:intron 35.0%
! TTTATTGAATTTTGCCTGGG+TGG - chr4.2:9935765-9935784 MS.gene58147:intron 35.0%
!! ACATGCTCATGAGCTATTTT+CGG + chr4.2:9934631-9934650 None:intergenic 35.0%
!! CTTCTAAAGCTCAAACACAT+GGG + chr4.2:9935366-9935385 None:intergenic 35.0%
!! TGGTGTTTTTAATCCATTGC+AGG - chr4.2:9934017-9934036 MS.gene58147:intron 35.0%
!! TGTATGTTTGGTTCCACTTT+TGG + chr4.2:9934467-9934486 None:intergenic 35.0%
!!! TTTTGGTTTTTGGTAAGCCA+AGG - chr4.2:9934826-9934845 MS.gene58147:intron 35.0%
ACATGCAAACACATAGCCAA+AGG + chr4.2:9936008-9936027 None:intergenic 40.0%
AGAGTTAGGTCCTATGTGAA+AGG - chr4.2:9935034-9935053 MS.gene58147:intron 40.0%
AGTTAGAAGCTAGACCAAAC+TGG + chr4.2:9935095-9935114 None:intergenic 40.0%
ATGTATCAGTCCGATCTTGA+TGG - chr4.2:9935824-9935843 MS.gene58147:intron 40.0%
CAAAAACTCTCCTTTCAAGC+CGG - chr4.2:9933634-9933653 MS.gene58147:CDS 40.0%
CAGCCATATCATTTAGCACA+GGG - chr4.2:9936249-9936268 MS.gene58147:intron 40.0%
CAGTATGGAAAATCATGACC+AGG + chr4.2:9936408-9936427 None:intergenic 40.0%
CCGGATGTAGATATAACCTT+TGG - chr4.2:9935989-9936008 MS.gene58147:CDS 40.0%
CTATAGGCAATGATAGACCT+GGG + chr4.2:9934740-9934759 None:intergenic 40.0%
GAGTTAGGTCCTATGTGAAA+GGG - chr4.2:9935035-9935054 MS.gene58147:intron 40.0%
GATGACATGACATTACTCGA+AGG + chr4.2:9936282-9936301 None:intergenic 40.0%
GGATCAAACTCCCAAGTAAA+AGG - chr4.2:9934887-9934906 MS.gene58147:intron 40.0%
GGGGTAAAAGAAGAAGAAGA+AGG + chr4.2:9933688-9933707 None:intergenic 40.0%
GTACTTCCAAATGCATTGGA+CGG + chr4.2:9936135-9936154 None:intergenic 40.0%
GTGCTAAATGATATGGCTGA+AGG + chr4.2:9936248-9936267 None:intergenic 40.0%
TATAGGCAATGATAGACCTG+GGG + chr4.2:9934739-9934758 None:intergenic 40.0%
TCAGCCATATCATTTAGCAC+AGG - chr4.2:9936248-9936267 MS.gene58147:intron 40.0%
TCGAGTAATGTCATGTCATC+GGG - chr4.2:9936282-9936301 MS.gene58147:intron 40.0%
TGATATAGTCTCTCACTTGC+AGG - chr4.2:9934098-9934117 MS.gene58147:intron 40.0%
TGCAGGAGAAAATGTATCAG+TGG - chr4.2:9934034-9934053 MS.gene58147:intron 40.0%
TTCGAATGTCTCCTGATATC+CGG - chr4.2:9935970-9935989 MS.gene58147:CDS 40.0%
TTCTGTTGAATTGACTGCCT+TGG + chr4.2:9936072-9936091 None:intergenic 40.0%
! AATTTTGCCTCCTCCAAAAG+TGG - chr4.2:9934451-9934470 MS.gene58147:intron 40.0%
! ACAACTTTTCTCGCTTCACT+TGG - chr4.2:9935876-9935895 MS.gene58147:intron 40.0%
! CACCAAAAGTATAACCGACA+CGG + chr4.2:9934947-9934966 None:intergenic 40.0%
!! ATGTTTGGTTCCACTTTTGG+AGG + chr4.2:9934464-9934483 None:intergenic 40.0%
!! GCTTCTAAAGCTCAAACACA+TGG + chr4.2:9935367-9935386 None:intergenic 40.0%
AAAGTATAACCGACACGGTG+TGG + chr4.2:9934942-9934961 None:intergenic 45.0%
AATGTCATGTCATCGGGGAA+CGG - chr4.2:9936288-9936307 MS.gene58147:intron 45.0%
AGGAATCACCAGTTTCTCCA+AGG - chr4.2:9936052-9936071 MS.gene58147:intron 45.0%
AGTGCAGCTTAAGTCCAGTT+TGG - chr4.2:9935078-9935097 MS.gene58147:intron 45.0%
ATGCATGTCCATGCAATCAC+TGG + chr4.2:9936376-9936395 None:intergenic 45.0%
ATTGACTGCCTTGGAGAAAC+TGG + chr4.2:9936063-9936082 None:intergenic 45.0%
CAAAATTAGGGGCTGGAATG+TGG + chr4.2:9933929-9933948 None:intergenic 45.0%
CGAGTAATGTCATGTCATCG+GGG - chr4.2:9936283-9936302 MS.gene58147:intron 45.0%
CGGAACCCGTAACAATGAAA+TGG - chr4.2:9936308-9936327 MS.gene58147:intron 45.0%
CTGCCCTGTGCTAAATGATA+TGG + chr4.2:9936255-9936274 None:intergenic 45.0%
GCTATAGGCAATGATAGACC+TGG + chr4.2:9934741-9934760 None:intergenic 45.0%
TAGGTCCTATGTGAAAGGGA+GGG - chr4.2:9935039-9935058 MS.gene58147:intron 45.0%
TCAAACGAGTCAGTCCAAAC+AGG - chr4.2:9934713-9934732 MS.gene58147:intron 45.0%
TCCAAATGCATTGGACGGTA+TGG + chr4.2:9936130-9936149 None:intergenic 45.0%
TCCATACCGTCCAATGCATT+TGG - chr4.2:9936126-9936145 MS.gene58147:intron 45.0%
TGAGCTTGAATATGGAGCAG+AGG - chr4.2:9936032-9936051 MS.gene58147:intron 45.0%
TTAGGTCCTATGTGAAAGGG+AGG - chr4.2:9935038-9935057 MS.gene58147:intron 45.0%
! TTGCCTGGGTGGGATTTTTA+TGG - chr4.2:9935776-9935795 MS.gene58147:intron 45.0%
!! GCATTTGGAAGTACTGTTGC+AGG - chr4.2:9936141-9936160 MS.gene58147:intron 45.0%
!! TTTGGTTCCACTTTTGGAGG+AGG + chr4.2:9934461-9934480 None:intergenic 45.0%
!!! CACCGTGTCGGTTATACTTT+TGG - chr4.2:9934942-9934961 MS.gene58147:intron 45.0%
!! AAATATTATAAACAAAATTA+GGG + chr4.2:9933941-9933960 None:intergenic 5.0%
ACTTGGGAGTTTGATCCCGA+GGG + chr4.2:9934884-9934903 None:intergenic 50.0%
AGGTCCTATGTGAAAGGGAG+GGG - chr4.2:9935040-9935059 MS.gene58147:intron 50.0%
GAATCTCTGTTCTGGCCACT+AGG + chr4.2:9936339-9936358 None:intergenic 50.0%
GTCCAACTCCAGTGATTGCA+TGG - chr4.2:9936365-9936384 MS.gene58147:intron 50.0%
GTCCATGCAATCACTGGAGT+TGG + chr4.2:9936370-9936389 None:intergenic 50.0%
GTGCCATAAAAATCCCACCC+AGG + chr4.2:9935782-9935801 None:intergenic 50.0%
TACTTGGGAGTTTGATCCCG+AGG + chr4.2:9934885-9934904 None:intergenic 50.0%
TGCATTGGACGGTATGGATG+CGG + chr4.2:9936124-9936143 None:intergenic 50.0%
! GAAGGAAGCATACAAGAGCG+TGG + chr4.2:9933666-9933685 None:intergenic 50.0%
!! GAAGTACTGTTGCAGGACAG+GGG - chr4.2:9936148-9936167 MS.gene58147:intron 50.0%
!! GGAAGTACTGTTGCAGGACA+GGG - chr4.2:9936147-9936166 MS.gene58147:intron 50.0%
!! TGGAAGTACTGTTGCAGGAC+AGG - chr4.2:9936146-9936165 MS.gene58147:intron 50.0%
AAGCCCCCTCCCTTTCACAT+AGG + chr4.2:9935047-9935066 None:intergenic 55.0%
CAGGACAGGGGATACGAACT+GGG - chr4.2:9936160-9936179 MS.gene58147:intron 55.0%
GACATGCATGCAGACAGTCC+TGG - chr4.2:9936387-9936406 MS.gene58147:CDS 55.0%
GCTGGGGAGATTAATCCCTC+GGG - chr4.2:9934866-9934885 MS.gene58147:intron 55.0%
GGCTGGGGAGATTAATCCCT+CGG - chr4.2:9934865-9934884 MS.gene58147:intron 55.0%
GGTCCTATGTGAAAGGGAGG+GGG - chr4.2:9935041-9935060 MS.gene58147:intron 55.0%
TATGTGAAAGGGAGGGGGCT+TGG - chr4.2:9935046-9935065 MS.gene58147:intron 55.0%
TCACTTGTGCCACACCGTGT+CGG - chr4.2:9934930-9934949 MS.gene58147:intron 55.0%
TGATAGACCTGGGGCCTGTT+TGG + chr4.2:9934730-9934749 None:intergenic 55.0%
! CAAGGTATCATCCCCTCGCA+CGG - chr4.2:9934844-9934863 MS.gene58147:intron 55.0%
! TACAAGAGCGTGGCGTTCAC+CGG + chr4.2:9933656-9933675 None:intergenic 55.0%
AGTCAGTCCAAACAGGCCCC+AGG - chr4.2:9934720-9934739 MS.gene58147:intron 60.0%
CGTGCGAGGGGATGATACCT+TGG + chr4.2:9934846-9934865 None:intergenic 60.0%
GCAGGACAGGGGATACGAAC+TGG - chr4.2:9936159-9936178 MS.gene58147:intron 60.0%
GCTGAAGGCTGCGACGAATG+GGG + chr4.2:9936233-9936252 None:intergenic 60.0%
GGCTGAAGGCTGCGACGAAT+GGG + chr4.2:9936234-9936253 None:intergenic 60.0%
TAATCTCCCCAGCCGTGCGA+GGG + chr4.2:9934859-9934878 None:intergenic 60.0%
TATCATCCCCTCGCACGGCT+GGG - chr4.2:9934849-9934868 MS.gene58147:intron 60.0%
TGGCTGAAGGCTGCGACGAA+TGG + chr4.2:9936235-9936254 None:intergenic 60.0%
TTAATCTCCCCAGCCGTGCG+AGG + chr4.2:9934860-9934879 None:intergenic 60.0%
!! GTGGCGTTCACCGGCTTGAA+AGG + chr4.2:9933647-9933666 None:intergenic 60.0%
AATCTCCCCAGCCGTGCGAG+GGG + chr4.2:9934858-9934877 None:intergenic 65.0%
ATCATCCCCTCGCACGGCTG+GGG - chr4.2:9934850-9934869 MS.gene58147:intron 65.0%
GTATCATCCCCTCGCACGGC+TGG - chr4.2:9934848-9934867 MS.gene58147:intron 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 9933625 9936428 9933625 ID=MS.gene58147
chr4.2 mRNA 9933625 9936428 9933625 ID=MS.gene58147.t1;Parent=MS.gene58147
chr4.2 exon 9936381 9936428 9936381 ID=MS.gene58147.t1.exon1;Parent=MS.gene58147.t1
chr4.2 CDS 9936381 9936428 9936381 ID=cds.MS.gene58147.t1;Parent=MS.gene58147.t1
chr4.2 exon 9935934 9936014 9935934 ID=MS.gene58147.t1.exon2;Parent=MS.gene58147.t1
chr4.2 CDS 9935934 9936014 9935934 ID=cds.MS.gene58147.t1;Parent=MS.gene58147.t1
chr4.2 exon 9933625 9934023 9933625 ID=MS.gene58147.t1.exon3;Parent=MS.gene58147.t1
chr4.2 CDS 9933625 9934023 9933625 ID=cds.MS.gene58147.t1;Parent=MS.gene58147.t1
Gene Sequence

>MS.gene58147

ATGAATCAACAAAAACTCTCCTTTCAAGCCGGTGAACGCCACGCTCTTGTATGCTTCCTTCCTTCTTCTTCTTCTTTTACCCCTTTCTTATTTTCAAATTATTATTCAATCAACAATTAATAATTTATTATACACGCAATCTCCATTCTTTTCTTTTAATTATTTAATTAATTAATTAATTATAATTATTATAGTTTTTAAGCTAAACAGTACCAGATTTCGTTCTTGCATTTTAGGGTTATTCAATTTCCCATTAATCTGTGACTTTACTAATTAAATTTTAATTCTTGAAGTAATTTCACCACATTCCAGCCCCTAATTTTGTTTATAATATTTGATGTTACTCAATGGTTGAAATTTTGTTTATTTATTTGATTTAATATAAAATGCTTTGGTGTTTTTAATCCATTGCAGGAGAAAATGTATCAGTGGATTCTTAGACAACAACAAGAAGGAATGAGAGTCACTACTATTGATATAGTCTCTCACTTGCAGGTTTTGTTTTATTCTCTATGCATTGTTGGGTTCTTTTTGGGTTTTTGTGCTGAACCATTACTCATTCAATGGAAAAACTACTTAGGGCCCTGTTTGAATAAACAATTTAGTGTATGTTTGATTGTGCCGTAAAAAAATGATTTTGAGTGAATTGATTTATATTTGATATCTTGATGTGAAGGTGTGTTGAACAATACATTTGAGTGTGAAAATAAACTCTAGAATCAGAAGTTACAATTTCTAGCTTCAAGTAGAAACAATTTTGGGGGCAAAATCAATTATACTCTAGAGGAACCAAACATGTCAAATTCAACTCTACACCTATTGAATCAATTTTGCCTCCTCCAAAAGTGGAACCAAACATACACTTATTTGTAGCACAAGCCCTTATAGTATAAGCACTTATGAATAAGATATTTTATAACACAGGATATAATGAAGTCAATTTGTTTTTGTATAATCTATAAGCTGTTCTCATAAACTATCCTGAAAAACTCATGGAAATAAGCCGAAAATAGCTCATGAGCATGTTGTAAGTTGTTTTCATAAGTTCTCCCGAACAATGTCACAAATGCTTATACCAGTAGATAAACTCAAACGAGTCAGTCCAAACAGGCCCCAGGTCTATCATTGCCTATAGCTTAAAGGTCACTTTGCCGATTGCTTTGAGTGTGAAACTGTTATTTAGTGGACATTATCTTTAAGCTTTTGGTTTTTGGTAAGCCAAGGTATCATCCCCTCGCACGGCTGGGGAGATTAATCCCTCGGGATCAAACTCCCAAGTAAAAGGTTGATAAACCCAAATATCTATCACTTGTGCCACACCGTGTCGGTTATACTTTTGGTGTTAGATTAGTCATGGATGATTGTACTAGAAAACTTGCCATTTTCCTGTCTGTATTATTTATTGCAGAAGAGTTAGGTCCTATGTGAAAGGGAGGGGGCTTGGAGTTGCTAAAGTGCAGCTTAAGTCCAGTTTGGTCTAGCTTCTAACTAATAGGAGCTTTTGAACTGAAAATAGTTTCTCAATGAACAGATGTGAGAAAAGTATATTAAAAGAATAATCTTGGAATAGTTATAAGCATGTAAACAAAAGTTCTAACGTAAGTTGAAACTGGCATCTGCATATCAGTTTTTTCATAAGCTCTCTTATTTAACTTCCTCATAAAATTATATAAGCTTAGTAGCTTGCGAAGATTTTTCTGCAATTGAACATCTATAAACTTATTTTAATCGAGAAACTCCCATGTGTTTGAGCTTTAGAAGCTAATTCAAGTTGGGCCTTAACTTATAAATCGTAAGGAAGTCCTTATGAGTTTTATAGGTGTGTATAGAGAAGTTAAACGAAAGAGCTTCTGAAATATTGACATGCAGAAGTTGATTTCAATTTTCATTATGAGTTTTATAGGTGTGAATAGAGAAGTTAAACGAAGAGCTTCTGAAATATTGACATGCAGAAGTTGATTTCAATTTTCAACTTATAAATGAAGTTGTTAGTTAAAATACTCATGGTTATTGCAAGATAATTTAAACCTTGCTTATTATCAGTATGTGTTTGCCGAAAAGTTTATCCATTTAACTCTAAGAGGTTGATTGCAACCACAATGTTAGTCATTGTATAATAGATTCACAATAAGTCCGGCCTTTTATTGAATTTTGCCTGGGTGGGATTTTTATGGCACCATAAGATTTTGCTCTAATGCTATGTATCAGTCCGATCTTGATGGTTGAACTATAGTCAAATCTTTGAAAAGATACAACTTTTCTCGCTTCACTTGGACCGTAGTTTCCCACATATTATGTCCTACAGTTAACATTATTGCACAATATCAATACAGAATAAATCTGTCTTCGAATGTCTCCTGATATCCGGATGTAGATATAACCTTTGGCTATGTGTTTGCATGTAGAATGAGCTTGAATATGGAGCAGAGGAATCACCAGTTTCTCCAAGGCAGTCAATTCAACAGAACTCCCAAACAGCAATTCAAACTAACTTTGCCGCATCCATACCGTCCAATGCATTTGGAAGTACTGTTGCAGGACAGGGGATACGAACTGGGCAAACCGATCAAGTAAAGAACTCAGTATTCTCAAATGCACTCTCTAGCCCCATTCGTCGCAGCCTTCAGCCATATCATTTAGCACAGGGCAGCAATCCTTCGAGTAATGTCATGTCATCGGGGAACGGAACCCGTAACAATGAAATGGTGTATCCTAGTGGCCAGAACAGAGATTCAAATTCGTCCAACTCCAGTGATTGCATGGACATGCATGCAGACAGTCCTGGTCATGATTTTCCATACTGA
Protein sequence

>MS.gene58147.t1

MNQQKLSFQAGERHALEKMYQWILRQQQEGMRVTTIDIVSHLQNELEYGAEESPVSPRQSIQQNSQTAIQTNFAASIPSNAFGSTVAGQGIRTGQTDQVKNSVFSNALSSPIRRSLQPYHLAQGSNPSSNVMSSGNGTRNNEMVYPSGQNRDSNSSNSSDCMDMHADSPGHDFPY