Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58185.t1 | XP_003610252.1 | 100 | 58 | 0 | 0 | 1 | 58 | 94 | 151 | 2.60E-23 | 117.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58185.t1 | G7JED5 | 100.0 | 58 | 0 | 0 | 1 | 58 | 94 | 151 | 1.9e-23 | 117.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060134 | MS.gene58185 | 0.805057 | 1.60E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 16 sgRNAs with CRISPR-Local
Find 110 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATCCTTTGGCTTGATTAA+AGG | 0.155831 | 8.4:+4389604 | MS.gene58185:CDS |
| GTCCTTGACAAACTTAGGTT+TGG | 0.351814 | 8.4:-4389796 | None:intergenic |
| CCATCATCTCTGGAAAGTTC+TGG | 0.397340 | 8.4:+4388554 | MS.gene58185:CDS |
| TTTGGAGCATACCAATCCTT+TGG | 0.411793 | 8.4:+4389592 | MS.gene58185:CDS |
| ACATGTCAGATACCAGCATT+TGG | 0.452980 | 8.4:+4389574 | MS.gene58185:intron |
| CAAGTCGCTTCCATCATCTC+TGG | 0.460619 | 8.4:+4388544 | MS.gene58185:CDS |
| GAGGGAAAAGATGGAGAAGA+AGG | 0.464994 | 8.4:+4389770 | MS.gene58185:CDS |
| TGAAAAGACCCCGCAAGAAG+AGG | 0.473639 | 8.4:+4389751 | MS.gene58185:CDS |
| CCAGAACTTTCCAGAGATGA+TGG | 0.477172 | 8.4:-4388554 | None:intergenic |
| CCGCAAGAAGAGGGAAAAGA+TGG | 0.502355 | 8.4:+4389761 | MS.gene58185:CDS |
| GAAAAGACCCCGCAAGAAGA+GGG | 0.506545 | 8.4:+4389752 | MS.gene58185:CDS |
| GATTGGTATGCTCCAAATGC+TGG | 0.509174 | 8.4:-4389586 | None:intergenic |
| CTTTAATCAAGCCAAAGGAT+TGG | 0.550609 | 8.4:-4389603 | None:intergenic |
| AAATCCTTTAATCAAGCCAA+AGG | 0.579388 | 8.4:-4389608 | None:intergenic |
| GTTCTGGTACATATATCTTG+TGG | 0.618251 | 8.4:+4388570 | MS.gene58185:CDS |
| GACCATACCTCTGAACCTTG+TGG | 0.656239 | 8.4:-4389634 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CTCTTTTAATTATTAATAAA+GGG | - | chr8.4:4389515-4389534 | None:intergenic | 10.0% |
| !!! | TCTCTTTTAATTATTAATAA+AGG | - | chr8.4:4389516-4389535 | None:intergenic | 10.0% |
| !! | GTTTATTTATGAATTATATC+AGG | + | chr8.4:4389356-4389375 | MS.gene58185:intron | 15.0% |
| !! | TTAAAGTTGTAATCAATATT+GGG | + | chr8.4:4389469-4389488 | MS.gene58185:intron | 15.0% |
| !! | ATATTGGGACATATATAAAA+TGG | + | chr8.4:4389484-4389503 | MS.gene58185:intron | 20.0% |
| !! | GTTAAAGTTGTAATCAATAT+TGG | + | chr8.4:4389468-4389487 | MS.gene58185:intron | 20.0% |
| !!! | AACCAGTAAGTATATATTTT+TGG | - | chr8.4:4389445-4389464 | None:intergenic | 20.0% |
| ! | ACTAATTATTTGCCATAAAG+AGG | + | chr8.4:4388794-4388813 | MS.gene58185:intron | 25.0% |
| ! | AGGGAAGTAAAAAATTGTAT+TGG | + | chr8.4:4388932-4388951 | MS.gene58185:intron | 25.0% |
| ! | AGTAAATCTAATGAACCTTT+AGG | - | chr8.4:4389172-4389191 | None:intergenic | 25.0% |
| ! | ATAAAGATCTATCATCTACA+AGG | + | chr8.4:4388729-4388748 | MS.gene58185:intron | 25.0% |
| ! | CTCCAAAAATATATACTTAC+TGG | + | chr8.4:4389440-4389459 | MS.gene58185:intron | 25.0% |
| !! | TATTGGAGAATGATTTTTGA+GGG | + | chr8.4:4388949-4388968 | MS.gene58185:intron | 25.0% |
| !!! | TCTTGGTAATTTTCACTTTT+AGG | + | chr8.4:4389661-4389680 | MS.gene58185:intron | 25.0% |
| AAATCCTTTAATCAAGCCAA+AGG | - | chr8.4:4389611-4389630 | None:intergenic | 30.0% | |
| TATTACTATGAACCTTTCTG+TGG | - | chr8.4:4389687-4389706 | None:intergenic | 30.0% | |
| TTTACTTATTAGTGCATGTC+TGG | + | chr8.4:4389186-4389205 | MS.gene58185:intron | 30.0% | |
| ! | ATTAAAGGATTTTTGCCACA+AGG | + | chr8.4:4389619-4389638 | MS.gene58185:CDS | 30.0% |
| ! | GTATTGGAGAATGATTTTTG+AGG | + | chr8.4:4388948-4388967 | MS.gene58185:intron | 30.0% |
| ! | TGCATTTTGTGATTAGGAAT+CGG | + | chr8.4:4389721-4389740 | MS.gene58185:intron | 30.0% |
| ! | TGTGTATGCATTTTGTGATT+AGG | + | chr8.4:4389715-4389734 | MS.gene58185:intron | 30.0% |
| !! | AATGATTTTTGAGGGTTTTG+GGG | + | chr8.4:4388957-4388976 | MS.gene58185:intron | 30.0% |
| !! | AGAATGATTTTTGAGGGTTT+TGG | + | chr8.4:4388955-4388974 | MS.gene58185:intron | 30.0% |
| !! | GAATGATTTTTGAGGGTTTT+GGG | + | chr8.4:4388956-4388975 | MS.gene58185:intron | 30.0% |
| !!! | GTGGTTTACTTTTAGAATGT+AGG | + | chr8.4:4388639-4388658 | MS.gene58185:intron | 30.0% |
| !!! | TTTTTTACTTCCCTCAAATG+AGG | - | chr8.4:4388926-4388945 | None:intergenic | 30.0% |
| AAACTTGAAGGAAAAGGGAT+TGG | + | chr8.4:4389251-4389270 | MS.gene58185:intron | 35.0% | |
| AACTTGAAGGAAAAGGGATT+GGG | + | chr8.4:4389252-4389271 | MS.gene58185:intron | 35.0% | |
| AAGAAATAGAGGACTTTGAG+AGG | + | chr8.4:4388856-4388875 | MS.gene58185:intron | 35.0% | |
| ACAAAATCCTCCTCATTTGA+GGG | + | chr8.4:4388913-4388932 | MS.gene58185:intron | 35.0% | |
| AGAGTAAGTGGAAGAAATAG+AGG | + | chr8.4:4388845-4388864 | MS.gene58185:intron | 35.0% | |
| AGCAACAAACTTGAAGGAAA+AGG | + | chr8.4:4389245-4389264 | MS.gene58185:intron | 35.0% | |
| CCATAAAGAGGAGTTATCTA+GGG | + | chr8.4:4388806-4388825 | MS.gene58185:intron | 35.0% | |
| CTTTAATCAAGCCAAAGGAT+TGG | - | chr8.4:4389606-4389625 | None:intergenic | 35.0% | |
| GCAACAAACTTGAAGGAAAA+GGG | + | chr8.4:4389246-4389265 | MS.gene58185:intron | 35.0% | |
| GGTAAAAAGGAAAGAGTAAG+TGG | + | chr8.4:4388833-4388852 | MS.gene58185:intron | 35.0% | |
| GTTCTGGTACATATATCTTG+TGG | + | chr8.4:4388570-4388589 | MS.gene58185:CDS | 35.0% | |
| TACAAAATCCTCCTCATTTG+AGG | + | chr8.4:4388912-4388931 | MS.gene58185:intron | 35.0% | |
| TTATGCTTGGCACAAAAATG+TGG | + | chr8.4:4388620-4388639 | MS.gene58185:intron | 35.0% | |
| ! | ACATTTTTGTGCCAAGCATA+AGG | - | chr8.4:4388621-4388640 | None:intergenic | 35.0% |
| ! | TAAGACTACGTTCTTTGAAG+TGG | - | chr8.4:4388706-4388725 | None:intergenic | 35.0% |
| ! | TATCTAGGGCTTTGGTAAAA+AGG | + | chr8.4:4388820-4388839 | MS.gene58185:intron | 35.0% |
| !! | ATGAACCTTTAGGCTTTGTT+TGG | - | chr8.4:4389162-4389181 | None:intergenic | 35.0% |
| !! | ATGATTTTTGAGGGTTTTGG+GGG | + | chr8.4:4388958-4388977 | MS.gene58185:intron | 35.0% |
| !! | CAATCCTTTGGCTTGATTAA+AGG | + | chr8.4:4389604-4389623 | MS.gene58185:CDS | 35.0% |
| !! | TGAACCTTTAGGCTTTGTTT+GGG | - | chr8.4:4389161-4389180 | None:intergenic | 35.0% |
| !! | TTCTTTGAAGTGGTACCAAT+TGG | - | chr8.4:4388696-4388715 | None:intergenic | 35.0% |
| AACTCCCAAACAAAGCCTAA+AGG | + | chr8.4:4389154-4389173 | MS.gene58185:intron | 40.0% | |
| AAGGTTACTTAGGAGCAGAA+TGG | - | chr8.4:4388602-4388621 | None:intergenic | 40.0% | |
| ACATGTCAGATACCAGCATT+TGG | + | chr8.4:4389574-4389593 | MS.gene58185:intron | 40.0% | |
| CCAGAAAGCAACAAACTTGA+AGG | + | chr8.4:4389239-4389258 | MS.gene58185:intron | 40.0% | |
| CCCTAGATAACTCCTCTTTA+TGG | - | chr8.4:4388809-4388828 | None:intergenic | 40.0% | |
| CTCCTAAGTAACCTTATGCT+TGG | + | chr8.4:4388607-4388626 | MS.gene58185:intron | 40.0% | |
| CTGTACAATCAACTGTGGTT+GGG | + | chr8.4:4389383-4389402 | MS.gene58185:intron | 40.0% | |
| GATCTATCATCTACAAGGCT+TGG | + | chr8.4:4388734-4388753 | MS.gene58185:intron | 40.0% | |
| GCCATAAAGAGGAGTTATCT+AGG | + | chr8.4:4388805-4388824 | MS.gene58185:intron | 40.0% | |
| GGTATCTGACATGTCATGAA+GGG | - | chr8.4:4389568-4389587 | None:intergenic | 40.0% | |
| GTGTTCTGTACAATCAACTG+TGG | + | chr8.4:4389378-4389397 | MS.gene58185:intron | 40.0% | |
| TCTGTACAATCAACTGTGGT+TGG | + | chr8.4:4389382-4389401 | MS.gene58185:intron | 40.0% | |
| TGCCAAGCATAAGGTTACTT+AGG | - | chr8.4:4388612-4388631 | None:intergenic | 40.0% | |
| TGGTATCTGACATGTCATGA+AGG | - | chr8.4:4389569-4389588 | None:intergenic | 40.0% | |
| TTGTAGTCACACACTCCAAT+TGG | + | chr8.4:4388678-4388697 | MS.gene58185:intron | 40.0% | |
| TTTACTTCCCTCAAATGAGG+AGG | - | chr8.4:4388923-4388942 | None:intergenic | 40.0% | |
| TTTGGGGGTGGAAAATATAG+GGG | - | chr8.4:4389116-4389135 | None:intergenic | 40.0% | |
| ! | ATTTTTGCCACAAGGTTCAG+AGG | + | chr8.4:4389627-4389646 | MS.gene58185:CDS | 40.0% |
| ! | CTTTGGGGGTGGAAAATATA+GGG | - | chr8.4:4389117-4389136 | None:intergenic | 40.0% |
| ! | TTTGGAGCATACCAATCCTT+TGG | + | chr8.4:4389592-4389611 | MS.gene58185:CDS | 40.0% |
| !! | CCTTCAAGTTTGTTGCTTTC+TGG | - | chr8.4:4389242-4389261 | None:intergenic | 40.0% |
| !! | TTAGGCTTTGTTTGGGAGTT+TGG | - | chr8.4:4389154-4389173 | None:intergenic | 40.0% |
| !! | TTCACTTTTAGGCCACAGAA+AGG | + | chr8.4:4389672-4389691 | MS.gene58185:intron | 40.0% |
| !!! | TGATTTTTGAGGGTTTTGGG+GGG | + | chr8.4:4388959-4388978 | MS.gene58185:intron | 40.0% |
| CCAGAACTTTCCAGAGATGA+TGG | - | chr8.4:4388557-4388576 | None:intergenic | 45.0% | |
| CCATTGTGAGAGAAGTCACT+AGG | - | chr8.4:4389329-4389348 | None:intergenic | 45.0% | |
| CCTAGTGACTTCTCTCACAA+TGG | + | chr8.4:4389326-4389345 | MS.gene58185:intron | 45.0% | |
| GAGGGAAAAGATGGAGAAGA+AGG | + | chr8.4:4389770-4389789 | MS.gene58185:CDS | 45.0% | |
| GATTGGTATGCTCCAAATGC+TGG | - | chr8.4:4389589-4389608 | None:intergenic | 45.0% | |
| TGCAACAACCTTCACTGACA+CGG | + | chr8.4:4389537-4389556 | MS.gene58185:intron | 45.0% | |
| TTTGAGAGGAGAGAGCTTTG+GGG | + | chr8.4:4388870-4388889 | MS.gene58185:intron | 45.0% | |
| ! | ACTTTGAGAGGAGAGAGCTT+TGG | + | chr8.4:4388868-4388887 | MS.gene58185:intron | 45.0% |
| ! | AGAGGAGTTATCTAGGGCTT+TGG | + | chr8.4:4388812-4388831 | MS.gene58185:intron | 45.0% |
| ! | ATCTTTTCCCTCTTCTTGCG+GGG | - | chr8.4:4389762-4389781 | None:intergenic | 45.0% |
| ! | CATCTTTTCCCTCTTCTTGC+GGG | - | chr8.4:4389763-4389782 | None:intergenic | 45.0% |
| ! | CCATCATCTCTGGAAAGTTC+TGG | + | chr8.4:4388554-4388573 | MS.gene58185:CDS | 45.0% |
| ! | CCATCTTTTCCCTCTTCTTG+CGG | - | chr8.4:4389764-4389783 | None:intergenic | 45.0% |
| ! | CTTTGAGAGGAGAGAGCTTT+GGG | + | chr8.4:4388869-4388888 | MS.gene58185:intron | 45.0% |
| ! | CTTTGTTTGGGAGTTTGGAG+GGG | - | chr8.4:4389149-4389168 | None:intergenic | 45.0% |
| !! | GCTTTGGGGGTGGAAAATAT+AGG | - | chr8.4:4389118-4389137 | None:intergenic | 45.0% |
| !! | GCTTTGTTTGGGAGTTTGGA+GGG | - | chr8.4:4389150-4389169 | None:intergenic | 45.0% |
| AAGGGATTGGGTCGCTAAAC+AGG | + | chr8.4:4389264-4389283 | MS.gene58185:intron | 50.0% | |
| CAAGTCGCTTCCATCATCTC+TGG | + | chr8.4:4388544-4388563 | MS.gene58185:CDS | 50.0% | |
| CCGCAAGAAGAGGGAAAAGA+TGG | + | chr8.4:4389761-4389780 | MS.gene58185:CDS | 50.0% | |
| GAAAAGACCCCGCAAGAAGA+GGG | + | chr8.4:4389752-4389771 | MS.gene58185:CDS | 50.0% | |
| GACCATACCTCTGAACCTTG+TGG | - | chr8.4:4389637-4389656 | None:intergenic | 50.0% | |
| TGAAAAGACCCCGCAAGAAG+AGG | + | chr8.4:4389751-4389770 | MS.gene58185:CDS | 50.0% | |
| TGCCACAAGGTTCAGAGGTA+TGG | + | chr8.4:4389632-4389651 | MS.gene58185:intron | 50.0% | |
| ! | CAGAGGTATGGTCAACGTCT+TGG | + | chr8.4:4389644-4389663 | MS.gene58185:intron | 50.0% |
| ! | TTGAGAGGAGAGAGCTTTGG+GGG | + | chr8.4:4388871-4388890 | MS.gene58185:intron | 50.0% |
| !! | GGCTTTGTTTGGGAGTTTGG+AGG | - | chr8.4:4389151-4389170 | None:intergenic | 50.0% |
| GGATTGGGTCGCTAAACAGG+AGG | + | chr8.4:4389267-4389286 | MS.gene58185:intron | 55.0% | |
| TGTTTGGGAGTTTGGAGGGG+AGG | - | chr8.4:4389146-4389165 | None:intergenic | 55.0% | |
| TTGGGTCGCTAAACAGGAGG+AGG | + | chr8.4:4389270-4389289 | MS.gene58185:intron | 55.0% | |
| !! | TGAGAGGAGAGAGCTTTGGG+GGG | + | chr8.4:4388872-4388891 | MS.gene58185:intron | 55.0% |
| GGAGTTTGGAGGGGAGGAGA+GGG | - | chr8.4:4389140-4389159 | None:intergenic | 60.0% | |
| GGGAGCAACCGTGTCAGTGA+AGG | - | chr8.4:4389548-4389567 | None:intergenic | 60.0% | |
| GAGGGGAGGAGAGGGCTTTG+GGG | - | chr8.4:4389132-4389151 | None:intergenic | 65.0% | |
| GGAGGGGAGGAGAGGGCTTT+GGG | - | chr8.4:4389133-4389152 | None:intergenic | 65.0% | |
| GGGAGTTTGGAGGGGAGGAG+AGG | - | chr8.4:4389141-4389160 | None:intergenic | 65.0% | |
| TGGAGGGGAGGAGAGGGCTT+TGG | - | chr8.4:4389134-4389153 | None:intergenic | 65.0% | |
| ! | AGGGGAGGAGAGGGCTTTGG+GGG | - | chr8.4:4389131-4389150 | None:intergenic | 65.0% |
| !! | GGAGGAGAGGGCTTTGGGGG+TGG | - | chr8.4:4389128-4389147 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 4388507 | 4389805 | 4388507 | ID=MS.gene58185 |
| chr8.4 | mRNA | 4388507 | 4389805 | 4388507 | ID=MS.gene58185.t1;Parent=MS.gene58185 |
| chr8.4 | exon | 4388507 | 4388591 | 4388507 | ID=MS.gene58185.t1.exon1;Parent=MS.gene58185.t1 |
| chr8.4 | CDS | 4388507 | 4388591 | 4388507 | ID=cds.MS.gene58185.t1;Parent=MS.gene58185.t1 |
| chr8.4 | exon | 4389583 | 4389648 | 4389583 | ID=MS.gene58185.t1.exon2;Parent=MS.gene58185.t1 |
| chr8.4 | CDS | 4389583 | 4389648 | 4389583 | ID=cds.MS.gene58185.t1;Parent=MS.gene58185.t1 |
| chr8.4 | exon | 4389737 | 4389805 | 4389737 | ID=MS.gene58185.t1.exon3;Parent=MS.gene58185.t1 |
| chr8.4 | CDS | 4389737 | 4389805 | 4389737 | ID=cds.MS.gene58185.t1;Parent=MS.gene58185.t1 |
| Gene Sequence |
| Protein sequence |
