AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene58274

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58274.t1 MTR_4g117890 95.074 203 10 0 1 203 2 204 9.60e-142 405
MS.gene58274.t1 MTR_0536s0020 87.685 203 25 0 1 203 7 209 5.19e-130 375
MS.gene58274.t1 MTR_0536s0030 78.286 175 32 1 36 204 4 178 4.73e-96 285
MS.gene58274.t1 MTR_4g118000 60.092 218 78 3 7 218 7 221 9.50e-81 249
MS.gene58274.t1 MTR_0536s0010 60.513 195 71 2 8 200 17 207 4.48e-76 237
MS.gene58274.t1 MTR_4g117980 53.883 206 85 3 8 203 7 212 8.77e-72 226
MS.gene58274.t1 MTR_4g117950 52.968 219 92 3 8 218 2 217 3.80e-71 224
MS.gene58274.t1 MTR_4g117960 48.889 225 101 3 8 218 7 231 8.23e-69 219
MS.gene58274.t1 MTR_2g034990 50.220 227 97 3 8 218 7 233 1.22e-68 218
MS.gene58274.t1 MTR_2g035020 49.029 206 95 2 8 203 7 212 1.74e-64 207
MS.gene58274.t1 MTR_5g029800 47.556 225 102 3 8 218 7 229 2.45e-61 199
MS.gene58274.t1 MTR_6g035265 51.000 200 91 2 8 203 7 203 4.17e-61 198
MS.gene58274.t1 MTR_7g012120 46.222 225 107 3 8 218 12 236 1.73e-57 189
MS.gene58274.t1 MTR_5g067170 46.018 226 106 4 8 218 7 231 7.82e-57 187
MS.gene58274.t1 MTR_6g035185 45.455 209 98 3 8 203 7 212 1.25e-52 176
MS.gene58274.t1 MTR_8g032950 44.690 226 112 3 6 218 6 231 9.45e-52 174
MS.gene58274.t1 MTR_4g031800 40.306 196 110 2 7 195 4 199 1.45e-47 163
MS.gene58274.t1 MTR_4g117970 48.092 131 64 1 77 203 53 183 3.18e-36 132
MS.gene58274.t1 MTR_7g055710 42.373 177 93 3 11 178 10 186 3.72e-36 133
MS.gene58274.t1 MTR_7g070860 38.342 193 107 3 7 190 2 191 3.73e-36 132
MS.gene58274.t1 MTR_6g035295 38.919 185 103 4 5 179 5 189 6.07e-36 132
MS.gene58274.t1 MTR_7g071050 42.077 183 101 3 1 178 1 183 9.10e-36 131
MS.gene58274.t1 MTR_7g070780 42.373 177 93 2 11 178 10 186 1.10e-35 131
MS.gene58274.t1 MTR_7g070930 41.243 177 95 2 11 178 13 189 1.22e-35 131
MS.gene58274.t1 MTR_7g070800 39.583 192 104 3 8 190 2 190 1.31e-35 131
MS.gene58274.t1 MTR_7g070660 40.113 177 97 2 11 178 10 186 3.01e-35 130
MS.gene58274.t1 MTR_7g070610 39.548 177 98 2 11 178 12 188 5.74e-35 129
MS.gene58274.t1 MTR_5g016660 34.498 229 129 4 3 215 1 224 8.77e-35 129
MS.gene58274.t1 MTR_7g070740 39.267 191 104 3 11 192 10 197 8.80e-35 129
MS.gene58274.t1 MTR_4g485630 34.783 207 121 4 7 200 4 209 1.74e-34 128
MS.gene58274.t1 MTR_7g071040 40.113 177 97 2 11 178 16 192 2.03e-34 128
MS.gene58274.t1 MTR_7g070940 36.792 212 118 3 5 200 2 213 3.68e-34 127
MS.gene58274.t1 MTR_4g108250 41.885 191 98 5 1 179 1 190 1.83e-33 125
MS.gene58274.t1 MTR_7g070910 38.418 177 100 2 11 178 10 186 3.55e-33 125
MS.gene58274.t1 MTR_7g070810 39.459 185 103 2 5 180 2 186 1.04e-31 120
MS.gene58274.t1 MTR_5g039900 34.973 183 110 2 5 178 6 188 1.60e-31 120
MS.gene58274.t1 MTR_2g035050 57.009 107 44 1 8 112 7 113 5.54e-30 108
MS.gene58274.t1 MTR_1g040310 39.011 182 101 3 7 179 10 190 1.19e-29 115
MS.gene58274.t1 MTR_8g066610 34.463 177 107 2 11 178 8 184 1.74e-29 114
MS.gene58274.t1 MTR_5g040030 36.364 187 109 5 5 181 7 193 4.44e-28 110
MS.gene58274.t1 MTR_5g045970 36.464 181 105 4 11 181 15 195 5.24e-28 110
MS.gene58274.t1 MTR_7g039570 34.759 187 112 4 5 181 7 193 5.30e-28 107
MS.gene58274.t1 MTR_7g117405 33.918 171 102 3 17 176 17 187 1.09e-27 109
MS.gene58274.t1 MTR_5g045960 33.690 187 112 4 7 181 4 190 3.10e-26 105
MS.gene58274.t1 MTR_1g062380 33.149 181 111 3 11 181 15 195 3.98e-26 105
MS.gene58274.t1 MTR_7g070870 35.484 186 110 3 3 180 10 193 4.14e-26 105
MS.gene58274.t1 MTR_7g102560 31.551 187 116 5 7 181 2 188 1.19e-25 103
MS.gene58274.t1 MTR_7g102450 30.357 224 118 6 17 217 16 224 1.35e-24 101
MS.gene58274.t1 MTR_4g485620 32.927 164 101 2 24 178 1 164 1.48e-24 100
MS.gene58274.t1 MTR_8g044140 39.865 148 79 3 40 178 5 151 8.07e-24 99.0
MS.gene58274.t1 MTR_4g485590 34.135 208 108 4 12 211 9 195 1.21e-23 97.8
MS.gene58274.t1 MTR_7g102520 28.856 201 127 5 11 195 11 211 2.96e-23 97.4
MS.gene58274.t1 MTR_4g485640 33.871 186 104 5 22 195 219 397 3.05e-23 97.8
MS.gene58274.t1 MTR_7g102550 30.332 211 131 6 16 211 13 222 7.99e-23 96.3
MS.gene58274.t1 MTR_2g035040 44.211 95 45 1 117 203 23 117 1.88e-21 91.7
MS.gene58274.t1 MTR_7g102460 28.804 184 118 4 11 181 8 191 7.07e-21 90.5
MS.gene58274.t1 MTR_7g470590 44.348 115 59 3 11 120 10 124 7.81e-21 84.7
MS.gene58274.t1 MTR_7g070570 44.348 115 59 3 11 120 10 124 7.81e-21 84.7
MS.gene58274.t1 MTR_4g024020 31.905 210 125 8 11 205 8 214 2.12e-20 89.4
MS.gene58274.t1 MTR_7g102490 30.811 185 111 6 13 181 8 191 6.04e-20 87.8
MS.gene58274.t1 MTR_2g098430 29.714 175 111 5 12 175 6 179 2.45e-15 74.7
MS.gene58274.t1 MTR_0009s0240 34.637 179 96 8 13 173 8 183 1.15e-14 72.8
MS.gene58274.t1 MTR_8g068340 35.570 149 89 4 1 146 1 145 1.17e-13 69.7
MS.gene58274.t1 MTR_8g068360 36.538 156 89 6 1 153 1 149 4.83e-13 67.8
MS.gene58274.t1 MTR_8g068330 35.714 140 83 4 7 143 3 138 1.23e-12 66.6
MS.gene58274.t1 MTR_8g071070 32.903 155 95 5 13 160 8 160 2.32e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58274.t1 AT4G34131 35.450 189 101 6 9 178 7 193 4.60e-31 119
MS.gene58274.t1 AT4G34135 30.556 216 127 5 9 201 8 223 4.60e-30 114
MS.gene58274.t1 AT2G15490 35.676 185 102 4 11 178 6 190 9.05e-30 112
MS.gene58274.t1 AT4G34135 30.556 216 127 5 9 201 8 223 1.89e-29 114
MS.gene58274.t1 AT2G15480 35.135 185 103 5 11 178 9 193 2.13e-28 112
MS.gene58274.t1 AT2G15480 35.135 185 103 5 11 178 9 193 2.68e-28 111
MS.gene58274.t1 AT3G53150 35.052 194 113 5 9 189 19 212 2.76e-28 111
MS.gene58274.t1 AT2G15490 35.676 185 102 4 11 178 6 190 3.39e-28 111
MS.gene58274.t1 AT2G15490 35.676 185 102 4 11 178 6 190 3.72e-28 111
MS.gene58274.t1 AT4G34138 34.409 186 106 5 7 176 6 191 1.17e-27 109
MS.gene58274.t1 AT2G36760 30.337 178 112 4 11 176 13 190 5.99e-26 105
MS.gene58274.t1 AT3G53160 34.637 179 103 5 11 176 7 184 1.06e-25 104
MS.gene58274.t1 AT2G36790 30.769 182 106 5 11 176 12 189 4.00e-25 102
MS.gene58274.t1 AT2G36780 31.148 183 106 4 10 176 12 190 3.22e-24 100
MS.gene58274.t1 AT2G36750 31.280 211 122 6 11 205 9 212 1.39e-23 98.2
MS.gene58274.t1 AT2G36770 30.769 182 106 4 11 176 13 190 2.47e-23 97.8
MS.gene58274.t1 AT2G36800 29.213 178 114 4 11 176 11 188 3.77e-22 94.4
MS.gene58274.t1 AT5G03490 33.103 145 92 4 13 154 20 162 7.63e-15 73.2
MS.gene58274.t1 AT5G49690 29.221 154 102 4 3 153 1 150 1.11e-13 69.7
MS.gene58274.t1 AT5G14860 28.834 163 95 4 17 162 13 171 2.09e-13 68.9
MS.gene58274.t1 AT1G51210 33.526 173 96 8 12 175 20 182 2.41e-12 65.9
MS.gene58274.t1 AT2G16890 25.478 157 111 2 12 162 9 165 1.07e-11 63.5
MS.gene58274.t1 AT2G16890 25.478 157 111 2 12 162 9 165 1.07e-11 63.5
MS.gene58274.t1 AT2G16890 26.282 156 109 3 13 162 10 165 1.19e-11 63.9
MS.gene58274.t1 AT2G16890 26.115 157 110 3 12 162 9 165 1.26e-11 63.5

Find 61 sgRNAs with CRISPR-Local

Find 140 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTGGCAGCTTGATAGATTT+TGG 0.242790 8.3:+8373301 None:intergenic
CTTCGGTGGTGCTGCATTCA+TGG 0.264342 8.3:+8373052 None:intergenic
AGACAACATGCTCGAGAGTT+TGG 0.291858 8.3:-8372258 MS.gene58274:CDS
ATACGGAGGTGTTGGTTTAA+AGG 0.314800 8.3:+8373410 None:intergenic
GACAACATGCTCGAGAGTTT+GGG 0.342872 8.3:-8372257 MS.gene58274:CDS
TACGGAGGTGTTGGTTTAAA+GGG 0.357603 8.3:+8373411 None:intergenic
GGCTGATTTCATGTATCCTT+GGG 0.372671 8.3:-8373222 MS.gene58274:CDS
ACCACCCATCCAACACTTCA+TGG 0.377818 8.3:-8373270 MS.gene58274:CDS
TGGCTGATTTCATGTATCCT+TGG 0.383559 8.3:-8373223 MS.gene58274:CDS
CCAAGGATTTGAGCATTAGA+AGG 0.388285 8.3:+8373443 None:intergenic
AAGAGGGGTTCGACACCATC+GGG 0.401462 8.3:+8373350 None:intergenic
GAGGTCGGTGGTGGTGAAGA+GGG 0.401972 8.3:+8373334 None:intergenic
AACAATTTACTCAGCTGCTT+CGG 0.411567 8.3:+8373035 None:intergenic
AGAGTTGCTATGTCACATAA+AGG 0.419759 8.3:+8373506 None:intergenic
GAAGCAGCTGAGTAAATTGT+TGG 0.445134 8.3:-8373033 MS.gene58274:CDS
CCTACTGCTTGTGAAGGAAA+TGG 0.454757 8.3:+8373377 None:intergenic
TCTGTTTGCTATTTGTGCCA+TGG 0.461379 8.3:-8373144 MS.gene58274:CDS
GAAGAGGGGTTCGACACCAT+CGG 0.468173 8.3:+8373349 None:intergenic
CTGCTCCATGAAGTGTTGGA+TGG 0.480259 8.3:+8373265 None:intergenic
AAATCAGCCACGATGCAATC+TGG 0.480396 8.3:+8373236 None:intergenic
CCTTCTAATGCTCAAATCCT+TGG 0.498454 8.3:-8373443 MS.gene58274:CDS
TTCATACCTTACTTAGCTCC+TGG 0.498644 8.3:-8373539 MS.gene58274:CDS
GAAATGGAACAGTGTGAATA+CGG 0.499028 8.3:+8373393 None:intergenic
GAGTTGCTATGTCACATAAA+GGG 0.502516 8.3:+8373507 None:intergenic
TGAGGTCGGTGGTGGTGAAG+AGG 0.507255 8.3:+8373333 None:intergenic
GAGGTTGTTGAGGTCGGTGG+TGG 0.509132 8.3:+8373325 None:intergenic
TGCTCCATGAAGTGTTGGAT+GGG 0.509428 8.3:+8373266 None:intergenic
TTTGGTGAGGTTGTTGAGGT+CGG 0.510671 8.3:+8373319 None:intergenic
ATTAGAAGGTGTGGTTATGA+TGG 0.516408 8.3:+8373457 None:intergenic
GGTGAGGTTGTTGAGGTCGG+TGG 0.516988 8.3:+8373322 None:intergenic
ACAAAGATGGATATGGAAGA+AGG 0.519937 8.3:-8373587 None:intergenic
CAGTGTGAATACGGAGGTGT+TGG 0.528928 8.3:+8373402 None:intergenic
CCATTTCCTTCACAAGCAGT+AGG 0.537343 8.3:-8373377 MS.gene58274:CDS
GTTGGAAGCGCTTCTTGAGA+CGG 0.542505 8.3:-8373015 MS.gene58274:CDS
GTGTGCTTTAACAGATTCCA+TGG 0.545350 8.3:+8373127 None:intergenic
AATTTACTCAGCTGCTTCGG+TGG 0.548377 8.3:+8373038 None:intergenic
ATATGACCAGGAGCTAAGTA+AGG 0.554657 8.3:+8373533 None:intergenic
GGTGCTGCTCCATGAAGTGT+TGG 0.556000 8.3:+8373261 None:intergenic
TTTGCAAGTTCATGAACCCA+AGG 0.571492 8.3:+8373206 None:intergenic
AAACCGTTGAAAGCAAGTCT+AGG 0.572799 8.3:+8373170 None:intergenic
TTAAAGGGATAGATTTGCCA+AGG 0.573034 8.3:+8373426 None:intergenic
TCCATGAAGTGTTGGATGGG+TGG 0.574411 8.3:+8373269 None:intergenic
GCTAAACATGCAATTCAAGA+AGG 0.576496 8.3:-8372228 MS.gene58274:CDS
GCAATAGAATGAGGAAGATG+AGG 0.586049 8.3:+8373074 None:intergenic
ATTCCTAGACTTGCTTTCAA+CGG 0.587622 8.3:-8373173 MS.gene58274:CDS
AGGTCGGTGGTGGTGAAGAG+GGG 0.588224 8.3:+8373335 None:intergenic
GATTTGAGCATTAGAAGGTG+TGG 0.592894 8.3:+8373448 None:intergenic
CATAAAGGGATCATATGACC+AGG 0.606218 8.3:+8373521 None:intergenic
TGAAGTGTTGGATGGGTGGT+CGG 0.608085 8.3:+8373273 None:intergenic
GCATTCATGGCAATAGAATG+AGG 0.608211 8.3:+8373065 None:intergenic
AAACATGCAATTCAAGAAGG+TGG 0.610247 8.3:-8372225 MS.gene58274:CDS
TCAGCCACGATGCAATCTGG+TGG 0.617343 8.3:+8373239 None:intergenic
GATGGGTGGTCGGAGTAGAG+TGG 0.624759 8.3:+8373283 None:intergenic
CAGCCACGATGCAATCTGGT+GGG 0.641127 8.3:+8373240 None:intergenic
GGGGTTCGACACCATCGGGG+AGG 0.641377 8.3:+8373354 None:intergenic
GGGAGGCCTACTGCTTGTGA+AGG 0.643475 8.3:+8373371 None:intergenic
CAAGCAGTAGGCCTCCCCGA+TGG 0.669962 8.3:-8373365 MS.gene58274:CDS
ACAACATGCTCGAGAGTTTG+GGG 0.670556 8.3:-8372256 MS.gene58274:CDS
AGAGGGGTTCGACACCATCG+GGG 0.732462 8.3:+8373351 None:intergenic
GCACCCACCAGATTGCATCG+TGG 0.747144 8.3:-8373243 MS.gene58274:CDS
ATGGAACAGTGTGAATACGG+AGG 0.749071 8.3:+8373396 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATCTCATAAAGTTGTTTATC+CGG + chr8.3:8373051-8373070 None:intergenic 25.0%
AAAGAAAAATGGTTGCCAAA+GGG - chr8.3:8373156-8373175 MS.gene58274:CDS 30.0%
AAGAAGGCTATGATTATTAG+GGG - chr8.3:8373204-8373223 MS.gene58274:CDS 30.0%
AGAAGAAGGCTATGATTATT+AGG - chr8.3:8373202-8373221 MS.gene58274:CDS 30.0%
ATGAAGAGGAGAAAGAAAAA+TGG - chr8.3:8373145-8373164 MS.gene58274:CDS 30.0%
GAAGAAGGCTATGATTATTA+GGG - chr8.3:8373203-8373222 MS.gene58274:CDS 30.0%
GAGAATTATTGGAAAGAAGA+AGG - chr8.3:8373188-8373207 MS.gene58274:CDS 30.0%
TAAAATTGTTCTCCATGCAT+CGG + chr8.3:8373345-8373364 None:intergenic 30.0%
! AACAATTTTGCTGAACTTGA+CGG - chr8.3:8372820-8372839 MS.gene58274:intron 30.0%
!! TTATGATGGTGACATTTTGA+CGG + chr8.3:8372318-8372337 None:intergenic 30.0%
AAACATGCAATTCAAGAAGG+TGG - chr8.3:8373561-8373580 MS.gene58274:CDS 35.0%
AAACTACTTGTCACAAAGCT+TGG - chr8.3:8372869-8372888 MS.gene58274:intron 35.0%
AACAATTTACTCAGCTGCTT+CGG + chr8.3:8372754-8372773 None:intergenic 35.0%
AACTTTATGAGATTGCATGC+GGG - chr8.3:8373058-8373077 MS.gene58274:CDS 35.0%
AAGAAAAATGGTTGCCAAAG+GGG - chr8.3:8373157-8373176 MS.gene58274:CDS 35.0%
AGAGTTGCTATGTCACATAA+AGG + chr8.3:8372283-8372302 None:intergenic 35.0%
ATCGAGTCACAAATTCGTAT+GGG - chr8.3:8373089-8373108 MS.gene58274:CDS 35.0%
CAACTTTATGAGATTGCATG+CGG - chr8.3:8373057-8373076 MS.gene58274:CDS 35.0%
GAAAGAAAAATGGTTGCCAA+AGG - chr8.3:8373155-8373174 MS.gene58274:CDS 35.0%
GAAATGGAACAGTGTGAATA+CGG + chr8.3:8372396-8372415 None:intergenic 35.0%
GAGTTGCTATGTCACATAAA+GGG + chr8.3:8372282-8372301 None:intergenic 35.0%
GCTAAACATGCAATTCAAGA+AGG - chr8.3:8373558-8373577 MS.gene58274:CDS 35.0%
GGGTTTGAAGAGAGAATTAT+TGG - chr8.3:8373177-8373196 MS.gene58274:CDS 35.0%
GTGCATTTATGACACATTGT+GGG - chr8.3:8373268-8373287 MS.gene58274:CDS 35.0%
GTTCAGCAAAATTGTTGACA+AGG + chr8.3:8372816-8372835 None:intergenic 35.0%
TTAAAGGGATAGATTTGCCA+AGG + chr8.3:8372363-8372382 None:intergenic 35.0%
TTGGAGGGATAATATTGAGA+AGG - chr8.3:8373461-8373480 MS.gene58274:CDS 35.0%
! ATTAGAAGGTGTGGTTATGA+TGG + chr8.3:8372332-8372351 None:intergenic 35.0%
! ATTCCTAGACTTGCTTTCAA+CGG - chr8.3:8372613-8372632 MS.gene58274:intron 35.0%
! GAGACGGTTTTCAAAAGTAA+CGG - chr8.3:8372787-8372806 MS.gene58274:intron 35.0%
!! CAATGAGAAGTTGATAACAC+AGG - chr8.3:8373365-8373384 MS.gene58274:CDS 35.0%
AAACCGTTGAAAGCAAGTCT+AGG + chr8.3:8372619-8372638 None:intergenic 40.0%
AACAGAGCATGACAATGAAG+AGG - chr8.3:8373131-8373150 MS.gene58274:CDS 40.0%
AATGGAGCTCAACTGGTATT+GGG - chr8.3:8373421-8373440 MS.gene58274:CDS 40.0%
AGGCTATGATTATTAGGGGT+TGG - chr8.3:8373208-8373227 MS.gene58274:CDS 40.0%
ATATGACCAGGAGCTAAGTA+AGG + chr8.3:8372256-8372275 None:intergenic 40.0%
ATGTGTCATAAATGCACCCA+CGG + chr8.3:8373265-8373284 None:intergenic 40.0%
ATTCAAGAGAAAGCAGAGAG+AGG - chr8.3:8372925-8372944 MS.gene58274:intron 40.0%
ATTCTCTCTTCAAACCCCTT+TGG + chr8.3:8373174-8373193 None:intergenic 40.0%
CATAAAGGGATCATATGACC+AGG + chr8.3:8372268-8372287 None:intergenic 40.0%
CATCGAGTCACAAATTCGTA+TGG - chr8.3:8373088-8373107 MS.gene58274:CDS 40.0%
CATTTATGACACATTGTGGG+TGG - chr8.3:8373271-8373290 MS.gene58274:CDS 40.0%
CCAAGGATTTGAGCATTAGA+AGG + chr8.3:8372346-8372365 None:intergenic 40.0%
CCTTCTAATGCTCAAATCCT+TGG - chr8.3:8372343-8372362 MS.gene58274:intron 40.0%
GAACTCGGAACAAGATAACT+CGG - chr8.3:8372990-8373009 MS.gene58274:intron 40.0%
GAAGCAGCTGAGTAAATTGT+TGG - chr8.3:8372753-8372772 MS.gene58274:intron 40.0%
GATTTGAGCATTAGAAGGTG+TGG + chr8.3:8372341-8372360 None:intergenic 40.0%
GCAATAGAATGAGGAAGATG+AGG + chr8.3:8372715-8372734 None:intergenic 40.0%
GCATTCATGGCAATAGAATG+AGG + chr8.3:8372724-8372743 None:intergenic 40.0%
GGCTATGATTATTAGGGGTT+GGG - chr8.3:8373209-8373228 MS.gene58274:CDS 40.0%
GGTGCATTTATGACACATTG+TGG - chr8.3:8373267-8373286 MS.gene58274:CDS 40.0%
GTGTGCTTTAACAGATTCCA+TGG + chr8.3:8372662-8372681 None:intergenic 40.0%
TTCATACCTTACTTAGCTCC+TGG - chr8.3:8372247-8372266 MS.gene58274:CDS 40.0%
TTTGCAAGTTCATGAACCCA+AGG + chr8.3:8372583-8372602 None:intergenic 40.0%
! AGTGGCAGCTTGATAGATTT+TGG + chr8.3:8372488-8372507 None:intergenic 40.0%
! GGCTGATTTCATGTATCCTT+GGG - chr8.3:8372564-8372583 MS.gene58274:intron 40.0%
! TGCTCTGTTCGTTTCTTTTC+AGG + chr8.3:8373120-8373139 None:intergenic 40.0%
! TGGCTGATTTCATGTATCCT+TGG - chr8.3:8372563-8372582 MS.gene58274:intron 40.0%
!! AAGTTGATAACACAGGTGCA+TGG - chr8.3:8373372-8373391 MS.gene58274:CDS 40.0%
!! AGATTTTGGTGAGGTTGTTG+AGG + chr8.3:8372474-8372493 None:intergenic 40.0%
!! AGTTGATAACACAGGTGCAT+GGG - chr8.3:8373373-8373392 MS.gene58274:CDS 40.0%
!! ATACGGAGGTGTTGGTTTAA+AGG + chr8.3:8372379-8372398 None:intergenic 40.0%
!! CAGCTTGATAGATTTTGGTG+AGG + chr8.3:8372483-8372502 None:intergenic 40.0%
!! TACGGAGGTGTTGGTTTAAA+GGG + chr8.3:8372378-8372397 None:intergenic 40.0%
!! TCTGTTTGCTATTTGTGCCA+TGG - chr8.3:8372642-8372661 MS.gene58274:intron 40.0%
!!! TGGAAGTCGATGTCATTTTC+CGG - chr8.3:8373029-8373048 MS.gene58274:CDS 40.0%
AAATCAGCCACGATGCAATC+TGG + chr8.3:8372553-8372572 None:intergenic 45.0%
AATTTACTCAGCTGCTTCGG+TGG + chr8.3:8372751-8372770 None:intergenic 45.0%
ACAACATGCTCGAGAGTTTG+GGG - chr8.3:8373530-8373549 MS.gene58274:CDS 45.0%
AGACAACATGCTCGAGAGTT+TGG - chr8.3:8373528-8373547 MS.gene58274:CDS 45.0%
ATAACACAGGTGCATGGGAT+TGG - chr8.3:8373378-8373397 MS.gene58274:CDS 45.0%
ATGGAACAGTGTGAATACGG+AGG + chr8.3:8372393-8372412 None:intergenic 45.0%
ATGGAGCTCAACTGGTATTG+GGG - chr8.3:8373422-8373441 MS.gene58274:CDS 45.0%
CCATTTCCTTCACAAGCAGT+AGG - chr8.3:8372409-8372428 MS.gene58274:intron 45.0%
CCTACTGCTTGTGAAGGAAA+TGG + chr8.3:8372412-8372431 None:intergenic 45.0%
GAATGGAGCTCAACTGGTAT+TGG - chr8.3:8373420-8373439 MS.gene58274:CDS 45.0%
GACAACATGCTCGAGAGTTT+GGG - chr8.3:8373529-8373548 MS.gene58274:CDS 45.0%
GCTCAGAATCATAACCTGTG+GGG + chr8.3:8373235-8373254 None:intergenic 45.0%
GGCTCAGAATCATAACCTGT+GGG + chr8.3:8373236-8373255 None:intergenic 45.0%
GTTCTGCGAATAAGAGAAGC+TGG + chr8.3:8372904-8372923 None:intergenic 45.0%
TAACACAGGTGCATGGGATT+GGG - chr8.3:8373379-8373398 MS.gene58274:CDS 45.0%
TATTGGGGAAAGAGAGAAGG+TGG - chr8.3:8373437-8373456 MS.gene58274:CDS 45.0%
TCGGTGGTGTACATATGCTT+TGG - chr8.3:8373009-8373028 MS.gene58274:CDS 45.0%
TGGCTCAGAATCATAACCTG+TGG + chr8.3:8373237-8373256 None:intergenic 45.0%
TGTCACAAAGCTTGGCATCT+TGG - chr8.3:8372877-8372896 MS.gene58274:intron 45.0%
TTTGGTGAGGTTGTTGAGGT+CGG + chr8.3:8372470-8372489 None:intergenic 45.0%
! AAAGAGAGAAGGTGGTTTGG+AGG - chr8.3:8373445-8373464 MS.gene58274:CDS 45.0%
! AAGAGAGAAGGTGGTTTGGA+GGG - chr8.3:8373446-8373465 MS.gene58274:CDS 45.0%
! TGGTATTGGGGAAAGAGAGA+AGG - chr8.3:8373434-8373453 MS.gene58274:CDS 45.0%
!! GTGTTTGAGTTGGCTGAACT+CGG - chr8.3:8372975-8372994 MS.gene58274:intron 45.0%
!! TGCTCCATGAAGTGTTGGAT+GGG + chr8.3:8372523-8372542 None:intergenic 45.0%
AACACAGGTGCATGGGATTG+GGG - chr8.3:8373380-8373399 MS.gene58274:CDS 50.0%
ACCACCCATCCAACACTTCA+TGG - chr8.3:8372516-8372535 MS.gene58274:intron 50.0%
CTCGGAACAAGATAACTCGG+TGG - chr8.3:8372993-8373012 MS.gene58274:intron 50.0%
GAAGGCTGTGAAGCGATTGA+TGG - chr8.3:8373479-8373498 MS.gene58274:CDS 50.0%
GCTGTGAAGCGATTGATGGA+CGG - chr8.3:8373483-8373502 MS.gene58274:CDS 50.0%
GGGAAAGAGAGAAGGTGGTT+TGG - chr8.3:8373442-8373461 MS.gene58274:CDS 50.0%
GTGTGCACGAGTGTTTGAGT+TGG - chr8.3:8372965-8372984 MS.gene58274:intron 50.0%
TGGGTGGAATTCCACTGTTG+AGG - chr8.3:8373287-8373306 MS.gene58274:CDS 50.0%
! CAGTGTGAATACGGAGGTGT+TGG + chr8.3:8372387-8372406 None:intergenic 50.0%
!! CTGCTCCATGAAGTGTTGGA+TGG + chr8.3:8372524-8372543 None:intergenic 50.0%
!! GGTGACATTTTGACGGCGTA+AGG + chr8.3:8372311-8372330 None:intergenic 50.0%
!! GTTGGAAGCGCTTCTTGAGA+CGG - chr8.3:8372771-8372790 MS.gene58274:intron 50.0%
!! TCCATGAAGTGTTGGATGGG+TGG + chr8.3:8372520-8372539 None:intergenic 50.0%
!! TGAAGTGTTGGATGGGTGGT+CGG + chr8.3:8372516-8372535 None:intergenic 50.0%
AAGAGGGGTTCGACACCATC+GGG + chr8.3:8372439-8372458 None:intergenic 55.0%
ACTGTTGAGGCAGTTAGCGC+AGG - chr8.3:8373300-8373319 MS.gene58274:CDS 55.0%
ATGATCACGTGGCCGATGCA+TGG - chr8.3:8373330-8373349 MS.gene58274:CDS 55.0%
ATTCTGAGCCACACAGCCGT+GGG - chr8.3:8373246-8373265 MS.gene58274:CDS 55.0%
CAGCCACGATGCAATCTGGT+GGG + chr8.3:8372549-8372568 None:intergenic 55.0%
CAGGGGTTCCAATGATCACG+TGG - chr8.3:8373319-8373338 MS.gene58274:CDS 55.0%
CTGTTGAGGCAGTTAGCGCA+GGG - chr8.3:8373301-8373320 MS.gene58274:CDS 55.0%
GAAGAGGGGTTCGACACCAT+CGG + chr8.3:8372440-8372459 None:intergenic 55.0%
GCAACGGAATGGAGCTCAAC+TGG - chr8.3:8373414-8373433 MS.gene58274:CDS 55.0%
TAAATGCACCCACGGCTGTG+TGG + chr8.3:8373257-8373276 None:intergenic 55.0%
TCAGCCACGATGCAATCTGG+TGG + chr8.3:8372550-8372569 None:intergenic 55.0%
TGCGCTAACTGCCTCAACAG+TGG + chr8.3:8373301-8373320 None:intergenic 55.0%
TGTTGAGGCAGTTAGCGCAG+GGG - chr8.3:8373302-8373321 MS.gene58274:CDS 55.0%
! GTGAAGCGATTGATGGACGG+TGG - chr8.3:8373486-8373505 MS.gene58274:CDS 55.0%
! TGCATCGGCCACGTGATCAT+TGG + chr8.3:8373330-8373349 None:intergenic 55.0%
!! CTTCGGTGGTGCTGCATTCA+TGG + chr8.3:8372737-8372756 None:intergenic 55.0%
!! GGTGCTGCTCCATGAAGTGT+TGG + chr8.3:8372528-8372547 None:intergenic 55.0%
ACAGGTGCATGGGATTGGGG+TGG - chr8.3:8373383-8373402 MS.gene58274:CDS 60.0%
AGAGGGGTTCGACACCATCG+GGG + chr8.3:8372438-8372457 None:intergenic 60.0%
CATGGGATTGGGGTGGAGGT+CGG - chr8.3:8373390-8373409 MS.gene58274:CDS 60.0%
GAGGTTGTTGAGGTCGGTGG+TGG + chr8.3:8372464-8372483 None:intergenic 60.0%
GATGGGTGGTCGGAGTAGAG+TGG + chr8.3:8372506-8372525 None:intergenic 60.0%
GATTCTGAGCCACACAGCCG+TGG - chr8.3:8373245-8373264 MS.gene58274:CDS 60.0%
GCACCCACCAGATTGCATCG+TGG - chr8.3:8372543-8372562 MS.gene58274:intron 60.0%
GGGAGGCCTACTGCTTGTGA+AGG + chr8.3:8372418-8372437 None:intergenic 60.0%
GGTGAGGTTGTTGAGGTCGG+TGG + chr8.3:8372467-8372486 None:intergenic 60.0%
TATTAGGGGTTGGGCCCCAC+AGG - chr8.3:8373218-8373237 MS.gene58274:CDS 60.0%
TGGAGGTCGGTGCAACGGAA+TGG - chr8.3:8373403-8373422 MS.gene58274:CDS 60.0%
! AGGTCGGTGGTGGTGAAGAG+GGG + chr8.3:8372454-8372473 None:intergenic 60.0%
! GAGGTCGGTGGTGGTGAAGA+GGG + chr8.3:8372455-8372474 None:intergenic 60.0%
! TGAGGTCGGTGGTGGTGAAG+AGG + chr8.3:8372456-8372475 None:intergenic 60.0%
CAAGCAGTAGGCCTCCCCGA+TGG - chr8.3:8372421-8372440 MS.gene58274:intron 65.0%
GGTGCATGGGATTGGGGTGG+AGG - chr8.3:8373386-8373405 MS.gene58274:CDS 65.0%
TGGGGTGGAGGTCGGTGCAA+CGG - chr8.3:8373398-8373417 MS.gene58274:CDS 65.0%
GGGGTTCGACACCATCGGGG+AGG + chr8.3:8372435-8372454 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 8372205 8373603 8372205 ID=MS.gene58274
chr8.3 mRNA 8372205 8373603 8372205 ID=MS.gene58274.t1;Parent=MS.gene58274
chr8.3 exon 8373006 8373603 8373006 ID=MS.gene58274.t1.exon1;Parent=MS.gene58274.t1
chr8.3 CDS 8373006 8373603 8373006 ID=cds.MS.gene58274.t1;Parent=MS.gene58274.t1
chr8.3 exon 8372205 8372278 8372205 ID=MS.gene58274.t1.exon2;Parent=MS.gene58274.t1
chr8.3 CDS 8372205 8372278 8372205 ID=cds.MS.gene58274.t1;Parent=MS.gene58274.t1
Gene Sequence

>MS.gene58274

ATGGATATGGAAGAAGGAGAAGAGAAAGCACTTAATATCTACTTCATACCTTACTTAGCTCCTGGTCATATGATCCCTTTATGTGACATAGCAACTCTCTTTGCCTTACGCCGTCAAAATGTCACCATCATAACCACACCTTCTAATGCTCAAATCCTTGGCAAATCTATCCCTTTAAACCAACACCTCCGTATTCACACTGTTCCATTTCCTTCACAAGCAGTAGGCCTCCCCGATGGTGTCGAACCCCTCTTCACCACCACCGACCTCAACAACCTCACCAAAATCTATCAAGCTGCCACTCTACTCCGACCACCCATCCAACACTTCATGGAGCAGCACCCACCAGATTGCATCGTGGCTGATTTCATGTATCCTTGGGTTCATGAACTTGCAAACAAGCTACAAATTCCTAGACTTGCTTTCAACGGTTTCTCTCTGTTTGCTATTTGTGCCATGGAATCTGTTAAAGCACACTCTCTTTATGAATCTGCTTCTTTTGTAATTCCTCATCTTCCTCATTCTATTGCCATGAATGCAGCACCACCGAAGCAGCTGAGTAAATTGTTGGAAGCGCTTCTTGAGACGGTTTTCAAAAGTAACGGAATCCTTGTCAACAATTTTGCTGAACTTGACGGTGAAGAGTATATCGAACACTACGAGAAAACTACTTGTCACAAAGCTTGGCATCTTGGTCCAGCTTCTCTTATTCGCAGAACTATTCAAGAGAAAGCAGAGAGAGGAGAAGAGAGTGTTGTGAGTGTGCACGAGTGTTTGAGTTGGCTGAACTCGGAACAAGATAACTCGGTGGTGTACATATGCTTTGGAAGTCGATGTCATTTTCCGGATAAACAACTTTATGAGATTGCATGCGGGATTGAAGCATCGAGTCACAAATTCGTATGGGTTGTTCCTGAAAAGAAACGAACAGAGCATGACAATGAAGAGGAGAAAGAAAAATGGTTGCCAAAGGGGTTTGAAGAGAGAATTATTGGAAAGAAGAAGGCTATGATTATTAGGGGTTGGGCCCCACAGGTTATGATTCTGAGCCACACAGCCGTGGGTGCATTTATGACACATTGTGGGTGGAATTCCACTGTTGAGGCAGTTAGCGCAGGGGTTCCAATGATCACGTGGCCGATGCATGGAGAACAATTTTACAATGAGAAGTTGATAACACAGGTGCATGGGATTGGGGTGGAGGTCGGTGCAACGGAATGGAGCTCAACTGGTATTGGGGAAAGAGAGAAGGTGGTTTGGAGGGATAATATTGAGAAGGCTGTGAAGCGATTGATGGACGGTGGTGATGAAGCAGAGAAAATCAGACAACATGCTCGAGAGTTTGGGGAGAAAGCTAAACATGCAATTCAAGAAGGTGGTTCATCTCATTCGAATTTAA
Protein sequence

>MS.gene58274.t1

MDMEEGEEKALNIYFIPYLAPGHMIPLCDIATLFALRRQNVTIITTPSNAQILGKSIPLNQHLRIHTVPFPSQAVGLPDGVEPLFTTTDLNNLTKIYQAATLLRPPIQHFMEQHPPDCIVADFMYPWVHELANKLQIPRLAFNGFSLFAICAMESVKAHSLYESASFVIPHLPHSIAMNAAPPKQLSKLLEALLETVFKNNMLESLGRKLNMQFKKVVHLIRI