Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58276.t1 | XP_003609436.1 | 74.7 | 304 | 45 | 2 | 54 | 325 | 57 | 360 | 3.50E-107 | 398.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58276.t1 | G7JFF7 | 74.7 | 304 | 45 | 2 | 54 | 325 | 57 | 360 | 2.5e-107 | 398.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057174 | MS.gene58276 | 0.800798 | 1.22E-48 | -1.69E-46 |
| MS.gene060935 | MS.gene58276 | 0.803306 | 3.71E-49 | -1.69E-46 |
| MS.gene060937 | MS.gene58276 | 0.802725 | 4.90E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58276.t1 | MTR_4g115690 | 81.214 | 346 | 33 | 2 | 12 | 325 | 15 | 360 | 0.0 | 540 |
| MS.gene58276.t1 | MTR_4g014150 | 71.179 | 229 | 37 | 4 | 37 | 253 | 34 | 245 | 8.61e-102 | 299 |
| MS.gene58276.t1 | MTR_3g106630 | 39.572 | 374 | 161 | 8 | 17 | 325 | 10 | 383 | 2.28e-73 | 231 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 58 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAAGTGATTATGGTGATTT+TGG | 0.193961 | 8.3:-10664997 | None:intergenic |
| ATGGAGTGTGTTCTTGAAAT+TGG | 0.276940 | 8.3:+10665690 | MS.gene58276:CDS |
| ATTCGAGCTTTGGTTGATAT+TGG | 0.291832 | 8.3:+10665525 | MS.gene58276:CDS |
| TTAAGGGTGAAATATTAGTT+AGG | 0.342748 | 8.3:+10665877 | MS.gene58276:CDS |
| GTTCTTGAAATTGGATATTT+CGG | 0.346217 | 8.3:+10665699 | MS.gene58276:CDS |
| TGAGAGAGGTTTGAATTAGT+TGG | 0.348856 | 8.3:-10665073 | None:intergenic |
| ACTTGAAGAAGTTGAGTGAT+TGG | 0.348987 | 8.3:-10664941 | None:intergenic |
| ATTTGTTGAGTTGCAGAGAA+TGG | 0.376884 | 8.3:+10665770 | MS.gene58276:CDS |
| AGCGAGATTTGCGGTGCAAT+TGG | 0.379941 | 8.3:+10665815 | MS.gene58276:CDS |
| TGGAATCTGAGAAGTGATTA+TGG | 0.386104 | 8.3:-10665006 | None:intergenic |
| CTTGAAGAAGTTGAGTGATT+GGG | 0.387558 | 8.3:-10664940 | None:intergenic |
| TTGCGGTGCAATTGGACGTT+GGG | 0.391803 | 8.3:+10665823 | MS.gene58276:CDS |
| TTCGGATTCGATTGCGGCGG+CGG | 0.391880 | 8.3:-10665200 | None:intergenic |
| CATATCGTTGTTGAATTCTC+AGG | 0.392680 | 8.3:+10665644 | MS.gene58276:CDS |
| ATTCGTCGGTGAAGTTTGTA+TGG | 0.398592 | 8.3:+10665494 | MS.gene58276:CDS |
| GGGTCCAACCATTCGAGCTT+TGG | 0.399580 | 8.3:+10665515 | MS.gene58276:CDS |
| GTGCAATTGGACGTTGGGGA+AGG | 0.421848 | 8.3:+10665828 | MS.gene58276:CDS |
| TTGGTTGATATTGGTTCTGT+TGG | 0.438975 | 8.3:+10665534 | MS.gene58276:CDS |
| TTGGAAGAATTGTGTGGAGT+TGG | 0.459432 | 8.3:-10665054 | None:intergenic |
| AGCTTTGGAGCGAGTTAACT+AGG | 0.463627 | 8.3:-10665117 | None:intergenic |
| GTCGATCCATGATTGAAGTT+CGG | 0.476587 | 8.3:-10665218 | None:intergenic |
| TAAGGGTGAAATATTAGTTA+GGG | 0.484187 | 8.3:+10665878 | MS.gene58276:CDS |
| GATTCAATTTCATCAACGAT+TGG | 0.487069 | 8.3:-10665600 | None:intergenic |
| TCGAATCCGAACTTCAATCA+TGG | 0.497527 | 8.3:+10665212 | MS.gene58276:CDS |
| GGAAGGAAGGAAGCTGTTGA+AGG | 0.498247 | 8.3:+10665720 | MS.gene58276:CDS |
| AGAGGGTGGTGACGGTTTCA+CGG | 0.504285 | 8.3:-10665240 | None:intergenic |
| GTTGATGGAATGAGATGAAA+GGG | 0.510249 | 8.3:-10665173 | None:intergenic |
| GTTGATGAAATTGAATCAAA+CGG | 0.517926 | 8.3:+10665606 | MS.gene58276:CDS |
| AGCTGTTGAAGGCATGTTGA+AGG | 0.520705 | 8.3:+10665731 | MS.gene58276:CDS |
| AATTGGATATTTCGGAAGGA+AGG | 0.527661 | 8.3:+10665707 | MS.gene58276:CDS |
| ACCCTCTCATGTCTCGTCTC+CGG | 0.531933 | 8.3:+10665256 | MS.gene58276:CDS |
| TGCAATTGGACGTTGGGGAA+GGG | 0.539508 | 8.3:+10665829 | MS.gene58276:CDS |
| TGTTGATGGAATGAGATGAA+AGG | 0.539703 | 8.3:-10665174 | None:intergenic |
| TTGAAATTGGATATTTCGGA+AGG | 0.544299 | 8.3:+10665703 | MS.gene58276:CDS |
| GAAATTGAGCTTATTAGCGA+TGG | 0.546186 | 8.3:+10665671 | MS.gene58276:CDS |
| TCAACCAAAGCTCGAATGGT+TGG | 0.549815 | 8.3:-10665519 | None:intergenic |
| TTCAACAAAGATGTATTCGT+CGG | 0.554859 | 8.3:+10665480 | MS.gene58276:CDS |
| TTGAAGTTCGGATTCGATTG+CGG | 0.559571 | 8.3:-10665206 | None:intergenic |
| TTCGTCGGTGAAGTTTGTAT+GGG | 0.560896 | 8.3:+10665495 | MS.gene58276:CDS |
| AATATCAACCAAAGCTCGAA+TGG | 0.564106 | 8.3:-10665523 | None:intergenic |
| GTTATGAACGATTTGAGACT+CGG | 0.567306 | 8.3:-10665145 | None:intergenic |
| GAGCTGTGTAGCGAGATTTG+CGG | 0.572894 | 8.3:+10665806 | MS.gene58276:CDS |
| GGTTAAGACAAAGAGAGAAG+AGG | 0.576455 | 8.3:+10665850 | MS.gene58276:CDS |
| GATTGTTTAGGTGTTGAGAG+AGG | 0.577383 | 8.3:-10665087 | None:intergenic |
| CCACAAAACTTCAGCAACAA+TGG | 0.581435 | 8.3:-10665937 | None:intergenic |
| TGCGGTGCAATTGGACGTTG+GGG | 0.585281 | 8.3:+10665824 | MS.gene58276:CDS |
| TTTGCGGTGCAATTGGACGT+TGG | 0.585765 | 8.3:+10665822 | MS.gene58276:CDS |
| GCTTTGGAGCGAGTTAACTA+GGG | 0.597907 | 8.3:-10665116 | None:intergenic |
| GCCGGAGACGAGACATGAGA+GGG | 0.609129 | 8.3:-10665257 | None:intergenic |
| GAGACATGAGAGGGTGGTGA+CGG | 0.611432 | 8.3:-10665248 | None:intergenic |
| GAAGCCTTGTGAGACTCTGT+CGG | 0.612490 | 8.3:-10665328 | None:intergenic |
| AATTAGTTGGAAGAATTGTG+TGG | 0.613623 | 8.3:-10665060 | None:intergenic |
| TGCCGGAGACGAGACATGAG+AGG | 0.646251 | 8.3:-10665258 | None:intergenic |
| TTCTCCGACAGAGTCTCACA+AGG | 0.647910 | 8.3:+10665324 | MS.gene58276:CDS |
| AAGTTCGGATTCGATTGCGG+CGG | 0.671573 | 8.3:-10665203 | None:intergenic |
| GTTGAAGGCATGTTGAAGGA+AGG | 0.684693 | 8.3:+10665735 | MS.gene58276:CDS |
| GGAGACGAGACATGAGAGGG+TGG | 0.691880 | 8.3:-10665254 | None:intergenic |
| TTGATGGAATGAGATGAAAG+GGG | 0.710167 | 8.3:-10665172 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GTTCTTGAAATTGGATATTT+CGG | + | chr8.3:10665699-10665718 | MS.gene58276:CDS | 25.0% |
| ! | TAAGGGTGAAATATTAGTTA+GGG | + | chr8.3:10665878-10665897 | MS.gene58276:CDS | 25.0% |
| ! | TTAAGGGTGAAATATTAGTT+AGG | + | chr8.3:10665877-10665896 | MS.gene58276:CDS | 25.0% |
| !! | GTTGATGAAATTGAATCAAA+CGG | + | chr8.3:10665606-10665625 | MS.gene58276:CDS | 25.0% |
| !! | TTCAACAACGATATGATTTT+CGG | - | chr8.3:10665639-10665658 | None:intergenic | 25.0% |
| AATTAGTTGGAAGAATTGTG+TGG | - | chr8.3:10665063-10665082 | None:intergenic | 30.0% | |
| GATTCAATTTCATCAACGAT+TGG | - | chr8.3:10665603-10665622 | None:intergenic | 30.0% | |
| TTCAACAAAGATGTATTCGT+CGG | + | chr8.3:10665480-10665499 | MS.gene58276:CDS | 30.0% | |
| TTGAAATTGGATATTTCGGA+AGG | + | chr8.3:10665703-10665722 | MS.gene58276:CDS | 30.0% | |
| ! | AGAAGTGATTATGGTGATTT+TGG | - | chr8.3:10665000-10665019 | None:intergenic | 30.0% |
| ! | TGCTTTTATGGAAGATGAAT+TGG | - | chr8.3:10665029-10665048 | None:intergenic | 30.0% |
| AATATCAACCAAAGCTCGAA+TGG | - | chr8.3:10665526-10665545 | None:intergenic | 35.0% | |
| AATTGGATATTTCGGAAGGA+AGG | + | chr8.3:10665707-10665726 | MS.gene58276:CDS | 35.0% | |
| ACTTGAAGAAGTTGAGTGAT+TGG | - | chr8.3:10664944-10664963 | None:intergenic | 35.0% | |
| ATGGAGTGTGTTCTTGAAAT+TGG | + | chr8.3:10665690-10665709 | MS.gene58276:CDS | 35.0% | |
| CATATCGTTGTTGAATTCTC+AGG | + | chr8.3:10665644-10665663 | MS.gene58276:CDS | 35.0% | |
| CTTGAAGAAGTTGAGTGATT+GGG | - | chr8.3:10664943-10664962 | None:intergenic | 35.0% | |
| GAAATTGAGCTTATTAGCGA+TGG | + | chr8.3:10665671-10665690 | MS.gene58276:CDS | 35.0% | |
| TGAGAGAGGTTTGAATTAGT+TGG | - | chr8.3:10665076-10665095 | None:intergenic | 35.0% | |
| TGGAATCTGAGAAGTGATTA+TGG | - | chr8.3:10665009-10665028 | None:intergenic | 35.0% | |
| TTGATGGAATGAGATGAAAG+GGG | - | chr8.3:10665175-10665194 | None:intergenic | 35.0% | |
| TTTGATCCGAAATGTTCTCA+CGG | + | chr8.3:10665414-10665433 | MS.gene58276:intron | 35.0% | |
| ! | AAGAGAGAAGAGGAGTTTTA+AGG | + | chr8.3:10665860-10665879 | MS.gene58276:CDS | 35.0% |
| ! | ATTCGAGCTTTGGTTGATAT+TGG | + | chr8.3:10665525-10665544 | MS.gene58276:CDS | 35.0% |
| ! | ATTTGTTGAGTTGCAGAGAA+TGG | + | chr8.3:10665770-10665789 | MS.gene58276:CDS | 35.0% |
| ! | GTTATGAACGATTTGAGACT+CGG | - | chr8.3:10665148-10665167 | None:intergenic | 35.0% |
| ! | GTTGATGGAATGAGATGAAA+GGG | - | chr8.3:10665176-10665195 | None:intergenic | 35.0% |
| ! | TGTTGATGGAATGAGATGAA+AGG | - | chr8.3:10665177-10665196 | None:intergenic | 35.0% |
| !! | AGAGAGAAGAGGAGTTTTAA+GGG | + | chr8.3:10665861-10665880 | MS.gene58276:CDS | 35.0% |
| !! | ATTGTTGCTGAAGTTTTGTG+GGG | + | chr8.3:10665939-10665958 | MS.gene58276:CDS | 35.0% |
| !! | CATTGTTGCTGAAGTTTTGT+GGG | + | chr8.3:10665938-10665957 | MS.gene58276:CDS | 35.0% |
| !! | TTGGTTGATATTGGTTCTGT+TGG | + | chr8.3:10665534-10665553 | MS.gene58276:CDS | 35.0% |
| !!! | CTAGGGTTTTGAGATTGTTT+AGG | - | chr8.3:10665102-10665121 | None:intergenic | 35.0% |
| !!! | GAGTTGGAAAAGTGCTTTTA+TGG | - | chr8.3:10665041-10665060 | None:intergenic | 35.0% |
| ATTCGTCGGTGAAGTTTGTA+TGG | + | chr8.3:10665494-10665513 | MS.gene58276:CDS | 40.0% | |
| CCACAAAACTTCAGCAACAA+TGG | - | chr8.3:10665940-10665959 | None:intergenic | 40.0% | |
| CGAATTCCGTGAGAACATTT+CGG | - | chr8.3:10665423-10665442 | None:intergenic | 40.0% | |
| GGTTAAGACAAAGAGAGAAG+AGG | + | chr8.3:10665850-10665869 | MS.gene58276:CDS | 40.0% | |
| GTCGATCCATGATTGAAGTT+CGG | - | chr8.3:10665221-10665240 | None:intergenic | 40.0% | |
| TCGAATCCGAACTTCAATCA+TGG | + | chr8.3:10665212-10665231 | MS.gene58276:CDS | 40.0% | |
| TTCGTCGGTGAAGTTTGTAT+GGG | + | chr8.3:10665495-10665514 | MS.gene58276:CDS | 40.0% | |
| TTGAAGTTCGGATTCGATTG+CGG | - | chr8.3:10665209-10665228 | None:intergenic | 40.0% | |
| TTGGAAGAATTGTGTGGAGT+TGG | - | chr8.3:10665057-10665076 | None:intergenic | 40.0% | |
| ! | CCATTGTTGCTGAAGTTTTG+TGG | + | chr8.3:10665937-10665956 | MS.gene58276:CDS | 40.0% |
| !! | GATTGTTTAGGTGTTGAGAG+AGG | - | chr8.3:10665090-10665109 | None:intergenic | 40.0% |
| !! | TTCGTTTTCGTTGTTGTTGC+CGG | - | chr8.3:10665278-10665297 | None:intergenic | 40.0% |
| GAACGAGTTGTTGTAACTCG+CGG | - | chr8.3:10665356-10665375 | None:intergenic | 45.0% | |
| TCAACCAAAGCTCGAATGGT+TGG | - | chr8.3:10665522-10665541 | None:intergenic | 45.0% | |
| ! | AGCTGTTGAAGGCATGTTGA+AGG | + | chr8.3:10665731-10665750 | MS.gene58276:CDS | 45.0% |
| ! | GTTGAAGGCATGTTGAAGGA+AGG | + | chr8.3:10665735-10665754 | MS.gene58276:CDS | 45.0% |
| !! | AGCTTTGGAGCGAGTTAACT+AGG | - | chr8.3:10665120-10665139 | None:intergenic | 45.0% |
| !! | GCTTTGGAGCGAGTTAACTA+GGG | - | chr8.3:10665119-10665138 | None:intergenic | 45.0% |
| !!! | TGAGACTCGGTTTTGAGCTT+TGG | - | chr8.3:10665135-10665154 | None:intergenic | 45.0% |
| AAGTTCGGATTCGATTGCGG+CGG | - | chr8.3:10665206-10665225 | None:intergenic | 50.0% | |
| AGCGAGATTTGCGGTGCAAT+TGG | + | chr8.3:10665815-10665834 | MS.gene58276:CDS | 50.0% | |
| GAGCTGTGTAGCGAGATTTG+CGG | + | chr8.3:10665806-10665825 | MS.gene58276:CDS | 50.0% | |
| GGAAGGAAGGAAGCTGTTGA+AGG | + | chr8.3:10665720-10665739 | MS.gene58276:CDS | 50.0% | |
| TGCAATTGGACGTTGGGGAA+GGG | + | chr8.3:10665829-10665848 | MS.gene58276:CDS | 50.0% | |
| TTCTCCGACAGAGTCTCACA+AGG | + | chr8.3:10665324-10665343 | MS.gene58276:CDS | 50.0% | |
| TTGCGGTGCAATTGGACGTT+GGG | + | chr8.3:10665823-10665842 | MS.gene58276:CDS | 50.0% | |
| TTTGCGGTGCAATTGGACGT+TGG | + | chr8.3:10665822-10665841 | MS.gene58276:CDS | 50.0% | |
| ! | GAAGCCTTGTGAGACTCTGT+CGG | - | chr8.3:10665331-10665350 | None:intergenic | 50.0% |
| ACCCTCTCATGTCTCGTCTC+CGG | + | chr8.3:10665256-10665275 | MS.gene58276:CDS | 55.0% | |
| CACGGAATTCGCGAACATGC+AGG | + | chr8.3:10665432-10665451 | MS.gene58276:intron | 55.0% | |
| GAGACATGAGAGGGTGGTGA+CGG | - | chr8.3:10665251-10665270 | None:intergenic | 55.0% | |
| GGGTCCAACCATTCGAGCTT+TGG | + | chr8.3:10665515-10665534 | MS.gene58276:CDS | 55.0% | |
| GTGCAATTGGACGTTGGGGA+AGG | + | chr8.3:10665828-10665847 | MS.gene58276:CDS | 55.0% | |
| TGCGGTGCAATTGGACGTTG+GGG | + | chr8.3:10665824-10665843 | MS.gene58276:CDS | 55.0% | |
| !! | AGAGGGTGGTGACGGTTTCA+CGG | - | chr8.3:10665243-10665262 | None:intergenic | 55.0% |
| GCCGGAGACGAGACATGAGA+GGG | - | chr8.3:10665260-10665279 | None:intergenic | 60.0% | |
| GGAGACGAGACATGAGAGGG+TGG | - | chr8.3:10665257-10665276 | None:intergenic | 60.0% | |
| TGCCGGAGACGAGACATGAG+AGG | - | chr8.3:10665261-10665280 | None:intergenic | 60.0% | |
| TTCGGATTCGATTGCGGCGG+CGG | - | chr8.3:10665203-10665222 | None:intergenic | 60.0% | |
| !!! | TGCGGCGGCGGTTTTGTTGA+TGG | - | chr8.3:10665191-10665210 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 10664902 | 10665974 | 10664902 | ID=MS.gene58276 |
| chr8.3 | mRNA | 10664902 | 10665974 | 10664902 | ID=MS.gene58276.t1;Parent=MS.gene58276 |
| chr8.3 | exon | 10664902 | 10665358 | 10664902 | ID=MS.gene58276.t1.exon1;Parent=MS.gene58276.t1 |
| chr8.3 | CDS | 10664902 | 10665358 | 10664902 | ID=cds.MS.gene58276.t1;Parent=MS.gene58276.t1 |
| chr8.3 | exon | 10665454 | 10665974 | 10665454 | ID=MS.gene58276.t1.exon2;Parent=MS.gene58276.t1 |
| chr8.3 | CDS | 10665454 | 10665974 | 10665454 | ID=cds.MS.gene58276.t1;Parent=MS.gene58276.t1 |
| Gene Sequence |
| Protein sequence |
