Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58307.t1 | XP_013444909.1 | 92.7 | 178 | 13 | 0 | 1 | 178 | 1 | 178 | 1.20E-88 | 335.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58307.t1 | Q58FX0 | 35.5 | 203 | 97 | 6 | 3 | 176 | 8 | 205 | 5.5e-23 | 109.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58307.t1 | I3SZG8 | 92.7 | 178 | 13 | 0 | 1 | 178 | 1 | 178 | 8.3e-89 | 335.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene06061 | MS.gene58307 | 0.819927 | 8.98E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58307.t1 | MTR_8g432370 | 92.697 | 178 | 13 | 0 | 1 | 178 | 1 | 178 | 1.30e-113 | 320 |
| MS.gene58307.t1 | MTR_8g432390 | 93.855 | 179 | 10 | 1 | 1 | 178 | 1 | 179 | 2.07e-113 | 320 |
| MS.gene58307.t1 | MTR_8g432350 | 92.179 | 179 | 13 | 1 | 1 | 178 | 1 | 179 | 6.27e-112 | 316 |
| MS.gene58307.t1 | MTR_8g432400 | 91.620 | 179 | 14 | 1 | 1 | 178 | 1 | 179 | 6.64e-109 | 308 |
| MS.gene58307.t1 | MTR_7g076000 | 51.648 | 182 | 67 | 6 | 1 | 177 | 1 | 166 | 9.37e-53 | 166 |
| MS.gene58307.t1 | MTR_8g432470 | 37.705 | 183 | 97 | 5 | 2 | 174 | 5 | 180 | 1.59e-28 | 104 |
| MS.gene58307.t1 | MTR_8g432440 | 36.066 | 183 | 100 | 5 | 2 | 174 | 5 | 180 | 1.12e-27 | 102 |
| MS.gene58307.t1 | MTR_8g432420 | 36.066 | 183 | 100 | 5 | 2 | 174 | 5 | 180 | 1.12e-27 | 102 |
| MS.gene58307.t1 | MTR_8g432450 | 36.158 | 177 | 98 | 3 | 2 | 168 | 5 | 176 | 4.29e-27 | 100 |
| MS.gene58307.t1 | MTR_8g432460 | 35.165 | 182 | 102 | 5 | 2 | 174 | 5 | 179 | 2.45e-24 | 94.0 |
| MS.gene58307.t1 | MTR_8g432490 | 35.165 | 182 | 102 | 5 | 2 | 174 | 5 | 179 | 1.36e-23 | 92.0 |
| MS.gene58307.t1 | MTR_3g064780 | 34.066 | 182 | 104 | 5 | 2 | 174 | 5 | 179 | 1.34e-21 | 88.2 |
| MS.gene58307.t1 | MTR_8g432480 | 32.530 | 166 | 98 | 4 | 2 | 156 | 7 | 169 | 1.09e-20 | 84.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58307.t1 | AT2G45760 | 35.122 | 205 | 99 | 6 | 1 | 176 | 6 | 205 | 2.04e-32 | 115 |
| MS.gene58307.t1 | AT3G61190 | 36.612 | 183 | 105 | 6 | 1 | 178 | 16 | 192 | 2.19e-27 | 102 |
| MS.gene58307.t1 | AT3G61190 | 36.612 | 183 | 105 | 6 | 1 | 178 | 16 | 192 | 2.19e-27 | 102 |
Find 45 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGAACAACAAAGCCATAAA+AGG | 0.217867 | 8.2:-65558831 | MS.gene58307:CDS |
| TACACTTTCAAATGTGTCTT+TGG | 0.278737 | 8.2:+65558358 | None:intergenic |
| TTATGGCTTTGTTGTTCTCT+TGG | 0.294333 | 8.2:+65558835 | None:intergenic |
| ATAATAGAGTTAGGAGAAAT+GGG | 0.321912 | 8.2:-65558542 | MS.gene58307:CDS |
| GATAATAGAGTTAGGAGAAA+TGG | 0.337385 | 8.2:-65558543 | MS.gene58307:CDS |
| CAACAGGGATTCCTGCTGTT+TGG | 0.341709 | 8.2:-65558389 | MS.gene58307:CDS |
| TCCTGCAGCAGAGTTGCAAC+AGG | 0.395408 | 8.2:-65558405 | MS.gene58307:CDS |
| AATTAGAACAAGAACTTTGT+TGG | 0.403293 | 8.2:+65558487 | None:intergenic |
| GTGAATGGAGTTCCGGTAAC+AGG | 0.406336 | 8.2:-65558450 | MS.gene58307:CDS |
| ATTAGAACAAGAACTTTGTT+GGG | 0.411878 | 8.2:+65558488 | None:intergenic |
| TCGATGTCATCGACGGTGAA+TGG | 0.414254 | 8.2:-65558465 | MS.gene58307:CDS |
| GTTACAAACCTTGCATGTAA+TGG | 0.418004 | 8.2:+65558693 | None:intergenic |
| GGCGTCCCGGTGGCCGGTAA+TGG | 0.421185 | 8.2:-65558429 | MS.gene58307:CDS |
| CACATACCCTCCAAGAAAAT+CGG | 0.422233 | 8.2:+65558605 | None:intergenic |
| AAGTAGTAGTAGTGTTGGTA+TGG | 0.432057 | 8.2:-65558643 | MS.gene58307:CDS |
| CCCTGTTGCAACTCTGCTGC+AGG | 0.438745 | 8.2:+65558404 | None:intergenic |
| GCCACCGGGACGCCTGTTAC+CGG | 0.441527 | 8.2:+65558438 | None:intergenic |
| TCTGCTGCAGGAGCCATTAC+CGG | 0.444058 | 8.2:+65558416 | None:intergenic |
| GGTTGTGGGATAATAGAGTT+AGG | 0.466052 | 8.2:-65558551 | MS.gene58307:CDS |
| ACCTTCGGAATCAAGTTTCG+TGG | 0.478987 | 8.2:+65558758 | None:intergenic |
| GTAACAGGCGTCCCGGTGGC+CGG | 0.481746 | 8.2:-65558435 | MS.gene58307:CDS |
| AGAGGAAGTAGTAGTAGTGT+TGG | 0.485422 | 8.2:-65558648 | MS.gene58307:CDS |
| TGGTAGTTTAACCAAACAGC+AGG | 0.487555 | 8.2:+65558378 | None:intergenic |
| CAGGAGCCATTACCGGCCAC+CGG | 0.491582 | 8.2:+65558423 | None:intergenic |
| TTCTAATTCGATGTCATCGA+CGG | 0.494293 | 8.2:-65558472 | MS.gene58307:CDS |
| AGAACAACAAAGCCATAAAA+GGG | 0.500399 | 8.2:-65558830 | MS.gene58307:CDS |
| TGTTGGTATGGCTAGAATAC+CGG | 0.506863 | 8.2:-65558631 | MS.gene58307:CDS |
| ATCGACGGTGAATGGAGTTC+CGG | 0.517982 | 8.2:-65558457 | MS.gene58307:CDS |
| CCTCCAAGAAAATCGGAAAC+CGG | 0.520232 | 8.2:+65558612 | None:intergenic |
| TGCAAGGTTTGTAACAATAG+AGG | 0.531016 | 8.2:-65558685 | MS.gene58307:CDS |
| AAGGTGGAAGTTATCCTACT+TGG | 0.531635 | 8.2:-65558740 | MS.gene58307:CDS |
| TCCTACTTGGAACGAAAAGT+TGG | 0.532011 | 8.2:-65558727 | MS.gene58307:CDS |
| AGTAGGATAACTTCCACCTT+CGG | 0.541429 | 8.2:+65558743 | None:intergenic |
| ACGAAACTTGATTCCGAAGG+TGG | 0.571494 | 8.2:-65558756 | MS.gene58307:CDS |
| AGGAGCCATTACCGGCCACC+GGG | 0.575429 | 8.2:+65558424 | None:intergenic |
| GGTTGTAAATATTTCAGTGA+AGG | 0.581610 | 8.2:-65558520 | MS.gene58307:CDS |
| AGTTCCGGTAACAGGCGTCC+CGG | 0.582783 | 8.2:-65558442 | MS.gene58307:CDS |
| TCGACGTGCCATTACATGCA+AGG | 0.603595 | 8.2:-65558701 | MS.gene58307:CDS |
| TTTGTCACGGTTCAATGTGA+CGG | 0.605787 | 8.2:-65558801 | MS.gene58307:CDS |
| GAGGTGAAGTACAAAACAAG+AGG | 0.611853 | 8.2:-65558666 | MS.gene58307:CDS |
| AAAAGGGAACACATTTGTCA+CGG | 0.633602 | 8.2:-65558814 | MS.gene58307:CDS |
| TAATAGAGTTAGGAGAAATG+GGG | 0.635926 | 8.2:-65558541 | MS.gene58307:CDS |
| ACCACGAAACTTGATTCCGA+AGG | 0.658333 | 8.2:-65558759 | MS.gene58307:CDS |
| TCCGGTAACAGGCGTCCCGG+TGG | 0.661367 | 8.2:-65558439 | MS.gene58307:CDS |
| CCTGCAGCAGAGTTGCAACA+GGG | 0.674047 | 8.2:-65558404 | MS.gene58307:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTGCAATTTTTAAGTTAT+AGG | - | chr8.2:65558672-65558691 | MS.gene58307:CDS | 15.0% |
| !!! | AATTTTTAAGTTATAGGTTG+TGG | - | chr8.2:65558678-65558697 | MS.gene58307:CDS | 20.0% |
| !!! | ATTTTTAAGTTATAGGTTGT+GGG | - | chr8.2:65558679-65558698 | MS.gene58307:CDS | 20.0% |
| ! | AATTAGAACAAGAACTTTGT+TGG | + | chr8.2:65558760-65558779 | None:intergenic | 25.0% |
| ! | ATAATAGAGTTAGGAGAAAT+GGG | - | chr8.2:65558702-65558721 | MS.gene58307:CDS | 25.0% |
| ! | ATTAGAACAAGAACTTTGTT+GGG | + | chr8.2:65558759-65558778 | None:intergenic | 25.0% |
| AGAACAACAAAGCCATAAAA+GGG | - | chr8.2:65558414-65558433 | MS.gene58307:CDS | 30.0% | |
| GATAATAGAGTTAGGAGAAA+TGG | - | chr8.2:65558701-65558720 | MS.gene58307:CDS | 30.0% | |
| GGTTGTAAATATTTCAGTGA+AGG | - | chr8.2:65558724-65558743 | MS.gene58307:CDS | 30.0% | |
| TAATAGAGTTAGGAGAAATG+GGG | - | chr8.2:65558703-65558722 | MS.gene58307:CDS | 30.0% | |
| AAAAGGGAACACATTTGTCA+CGG | - | chr8.2:65558430-65558449 | MS.gene58307:CDS | 35.0% | |
| GAGAACAACAAAGCCATAAA+AGG | - | chr8.2:65558413-65558432 | MS.gene58307:CDS | 35.0% | |
| GTTACAAACCTTGCATGTAA+TGG | + | chr8.2:65558554-65558573 | None:intergenic | 35.0% | |
| TGCAAGGTTTGTAACAATAG+AGG | - | chr8.2:65558559-65558578 | MS.gene58307:CDS | 35.0% | |
| TTCTAATTCGATGTCATCGA+CGG | - | chr8.2:65558772-65558791 | MS.gene58307:CDS | 35.0% | |
| ! | AGAGATGACTATTTTGTCAG+CGG | - | chr8.2:65558379-65558398 | MS.gene58307:CDS | 35.0% |
| ! | GACAAATGTGTTCCCTTTTA+TGG | + | chr8.2:65558429-65558448 | None:intergenic | 35.0% |
| !! | AAGTAGTAGTAGTGTTGGTA+TGG | - | chr8.2:65558601-65558620 | MS.gene58307:CDS | 35.0% |
| !! | TTATGGCTTTGTTGTTCTCT+TGG | + | chr8.2:65558412-65558431 | None:intergenic | 35.0% |
| AAGGTGGAAGTTATCCTACT+TGG | - | chr8.2:65558504-65558523 | MS.gene58307:CDS | 40.0% | |
| AGAGGAAGTAGTAGTAGTGT+TGG | - | chr8.2:65558596-65558615 | MS.gene58307:CDS | 40.0% | |
| AGTAGGATAACTTCCACCTT+CGG | + | chr8.2:65558504-65558523 | None:intergenic | 40.0% | |
| CACATACCCTCCAAGAAAAT+CGG | + | chr8.2:65558642-65558661 | None:intergenic | 40.0% | |
| GAGGTGAAGTACAAAACAAG+AGG | - | chr8.2:65558578-65558597 | MS.gene58307:CDS | 40.0% | |
| GGTTGTGGGATAATAGAGTT+AGG | - | chr8.2:65558693-65558712 | MS.gene58307:CDS | 40.0% | |
| TCCTACTTGGAACGAAAAGT+TGG | - | chr8.2:65558517-65558536 | MS.gene58307:CDS | 40.0% | |
| TGGTAGTTTAACCAAACAGC+AGG | + | chr8.2:65558869-65558888 | None:intergenic | 40.0% | |
| TGTTGGTATGGCTAGAATAC+CGG | - | chr8.2:65558613-65558632 | MS.gene58307:CDS | 40.0% | |
| TTTGTCACGGTTCAATGTGA+CGG | - | chr8.2:65558443-65558462 | MS.gene58307:CDS | 40.0% | |
| ! | ACCAACTTTTCGTTCCAAGT+AGG | + | chr8.2:65558521-65558540 | None:intergenic | 40.0% |
| !!! | ATACCGGTTTCCGATTTTCT+TGG | - | chr8.2:65558629-65558648 | MS.gene58307:CDS | 40.0% |
| ACCACGAAACTTGATTCCGA+AGG | - | chr8.2:65558485-65558504 | MS.gene58307:CDS | 45.0% | |
| ACCTTCGGAATCAAGTTTCG+TGG | + | chr8.2:65558489-65558508 | None:intergenic | 45.0% | |
| ACGAAACTTGATTCCGAAGG+TGG | - | chr8.2:65558488-65558507 | MS.gene58307:CDS | 45.0% | |
| CCTCCAAGAAAATCGGAAAC+CGG | + | chr8.2:65558635-65558654 | None:intergenic | 45.0% | |
| ! | CGGTTTCCGATTTTCTTGGA+GGG | - | chr8.2:65558633-65558652 | MS.gene58307:CDS | 45.0% |
| ATCGACGGTGAATGGAGTTC+CGG | - | chr8.2:65558787-65558806 | MS.gene58307:CDS | 50.0% | |
| CAACAGGGATTCCTGCTGTT+TGG | - | chr8.2:65558855-65558874 | MS.gene58307:CDS | 50.0% | |
| GTGAATGGAGTTCCGGTAAC+AGG | - | chr8.2:65558794-65558813 | MS.gene58307:CDS | 50.0% | |
| TCGATGTCATCGACGGTGAA+TGG | - | chr8.2:65558779-65558798 | MS.gene58307:CDS | 50.0% | |
| ! | CCGGTTTCCGATTTTCTTGG+AGG | - | chr8.2:65558632-65558651 | MS.gene58307:CDS | 50.0% |
| ! | TCGACGTGCCATTACATGCA+AGG | - | chr8.2:65558543-65558562 | MS.gene58307:CDS | 50.0% |
| CCTGCAGCAGAGTTGCAACA+GGG | - | chr8.2:65558840-65558859 | MS.gene58307:CDS | 55.0% | |
| TCCTGCAGCAGAGTTGCAAC+AGG | - | chr8.2:65558839-65558858 | MS.gene58307:CDS | 55.0% | |
| TCTGCTGCAGGAGCCATTAC+CGG | + | chr8.2:65558831-65558850 | None:intergenic | 55.0% | |
| AGTTCCGGTAACAGGCGTCC+CGG | - | chr8.2:65558802-65558821 | MS.gene58307:CDS | 60.0% | |
| CCCTGTTGCAACTCTGCTGC+AGG | + | chr8.2:65558843-65558862 | None:intergenic | 60.0% | |
| AGGAGCCATTACCGGCCACC+GGG | + | chr8.2:65558823-65558842 | None:intergenic | 65.0% | |
| CAGGAGCCATTACCGGCCAC+CGG | + | chr8.2:65558824-65558843 | None:intergenic | 65.0% | |
| GCCACCGGGACGCCTGTTAC+CGG | + | chr8.2:65558809-65558828 | None:intergenic | 70.0% | |
| GTAACAGGCGTCCCGGTGGC+CGG | - | chr8.2:65558809-65558828 | MS.gene58307:CDS | 70.0% | |
| TCCGGTAACAGGCGTCCCGG+TGG | - | chr8.2:65558805-65558824 | MS.gene58307:CDS | 70.0% | |
| GGCGTCCCGGTGGCCGGTAA+TGG | - | chr8.2:65558815-65558834 | MS.gene58307:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 65558365 | 65558901 | 65558365 | ID=MS.gene58307 |
| chr8.2 | mRNA | 65558365 | 65558901 | 65558365 | ID=MS.gene58307.t1;Parent=MS.gene58307 |
| chr8.2 | exon | 65558365 | 65558901 | 65558365 | ID=MS.gene58307.t1.exon1;Parent=MS.gene58307.t1 |
| chr8.2 | CDS | 65558365 | 65558901 | 65558365 | ID=cds.MS.gene58307.t1;Parent=MS.gene58307.t1 |
| Gene Sequence |
| Protein sequence |
