Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58407.t1 | XP_013457933.1 | 97.8 | 324 | 7 | 0 | 1 | 324 | 1 | 324 | 2.30E-167 | 598.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58407.t1 | A0A072UQ71 | 97.8 | 324 | 7 | 0 | 1 | 324 | 1 | 324 | 1.6e-167 | 598.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050649 | MS.gene58407 | 0.829605 | 4.69E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58407.t1 | MTR_4g109020 | 97.840 | 324 | 7 | 0 | 1 | 324 | 1 | 324 | 0.0 | 631 |
| MS.gene58407.t1 | MTR_3g037110 | 69.394 | 330 | 89 | 3 | 1 | 324 | 1 | 324 | 1.36e-153 | 433 |
| MS.gene58407.t1 | MTR_4g082350 | 28.082 | 292 | 198 | 9 | 6 | 294 | 49 | 331 | 4.53e-28 | 112 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58407.t1 | AT5G61340 | 46.687 | 332 | 157 | 8 | 1 | 323 | 5 | 325 | 6.26e-81 | 249 |
| MS.gene58407.t1 | AT5G61340 | 46.687 | 332 | 157 | 8 | 1 | 323 | 38 | 358 | 2.09e-80 | 249 |
| MS.gene58407.t1 | AT1G69430 | 29.667 | 300 | 183 | 10 | 6 | 294 | 45 | 327 | 2.71e-24 | 101 |
| MS.gene58407.t1 | AT1G26650 | 27.526 | 287 | 196 | 8 | 6 | 289 | 29 | 306 | 8.02e-24 | 100 |
Find 55 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCAAATTTATAACCGTTTA+AGG | 0.015328 | 8.3:-15611538 | MS.gene58407:CDS |
| TTTCAAGAAGTGGACAATTA+TGG | 0.284116 | 8.3:-15610777 | MS.gene58407:CDS |
| GAGTTTAAGTGCTTGAATTA+TGG | 0.313893 | 8.3:+15611379 | None:intergenic |
| TAAAGCTTGTATCTTGATAA+GGG | 0.315641 | 8.3:-15611051 | MS.gene58407:CDS |
| TTAAAGCTTGTATCTTGATA+AGG | 0.341052 | 8.3:-15611052 | MS.gene58407:CDS |
| AAGTGAAATAATGATAGTTT+TGG | 0.354262 | 8.3:+15611645 | None:intergenic |
| CAAGAAACAGTTGCAGATAT+AGG | 0.364089 | 8.3:+15611286 | None:intergenic |
| CTCCTCTTCATGTCAGCGAC+CGG | 0.364905 | 8.3:-15611176 | MS.gene58407:CDS |
| AATATGTCACTTGTTCTATC+TGG | 0.371364 | 8.3:-15611107 | MS.gene58407:CDS |
| GCAGCCAAGGCAATATTAAC+AGG | 0.394585 | 8.3:+15610996 | None:intergenic |
| GGTTATAAATTTGAGGCAAA+AGG | 0.402196 | 8.3:+15611546 | None:intergenic |
| TCCTCTTCATGTCAGCGACC+GGG | 0.410312 | 8.3:-15611175 | MS.gene58407:CDS |
| TGTTTCACTTCAGAATTGTA+AGG | 0.417611 | 8.3:-15610962 | MS.gene58407:CDS |
| GTAAGGGAATATCACATTGT+TGG | 0.423368 | 8.3:-15610945 | MS.gene58407:CDS |
| AATGTTGAACAATAGAGAAT+AGG | 0.432290 | 8.3:+15611487 | None:intergenic |
| CTTAAACGGTTATAAATTTG+AGG | 0.435128 | 8.3:+15611539 | None:intergenic |
| ATCCCCTCTAAAAGCACAAA+AGG | 0.440963 | 8.3:+15610912 | None:intergenic |
| ACTTCCTGTTAATATTGCCT+TGG | 0.454147 | 8.3:-15611000 | MS.gene58407:CDS |
| AGAAGGACAGTTAGTGTGAA+AGG | 0.454408 | 8.3:+15611416 | None:intergenic |
| GAGAATGAAGGTGGCAAAGA+TGG | 0.476193 | 8.3:+15611344 | None:intergenic |
| TTCAGCTTTGTTGAAACACT+TGG | 0.481261 | 8.3:-15611218 | MS.gene58407:CDS |
| GACATGAAGAGGAGGAAACT+AGG | 0.482045 | 8.3:+15611185 | None:intergenic |
| GTCACTTGTTCTATCTGGTA+TGG | 0.482263 | 8.3:-15611102 | MS.gene58407:CDS |
| GTTATAAATTTGAGGCAAAA+GGG | 0.486260 | 8.3:+15611547 | None:intergenic |
| AGGAGAATCATGCATTGGTC+AGG | 0.492954 | 8.3:-15610811 | MS.gene58407:CDS |
| ATGAGTATCGAAGCAGAGAA+AGG | 0.504345 | 8.3:+15611599 | None:intergenic |
| ATTTGAGGCAAAAGGGATGA+AGG | 0.511390 | 8.3:+15611554 | None:intergenic |
| CCTCTTCATGTCAGCGACCG+GGG | 0.524558 | 8.3:-15611174 | MS.gene58407:CDS |
| GGAATATCACATTGTTGGAA+AGG | 0.524960 | 8.3:-15610940 | MS.gene58407:CDS |
| GTGGACAATTATGGTTACAT+TGG | 0.525805 | 8.3:-15610768 | MS.gene58407:CDS |
| CAGATATAGGTGTGGAAGAG+TGG | 0.530195 | 8.3:+15611299 | None:intergenic |
| CAAGGCTTCAATAGCAGCCA+AGG | 0.531315 | 8.3:+15610983 | None:intergenic |
| GAAGGACAGTTAGTGTGAAA+GGG | 0.531755 | 8.3:+15611417 | None:intergenic |
| GGAAGGACATGGTGGATACT+CGG | 0.533045 | 8.3:-15611081 | MS.gene58407:CDS |
| GTTCAACATTCTTAACCTCA+AGG | 0.536846 | 8.3:-15611474 | MS.gene58407:CDS |
| CCCCGGTCGCTGACATGAAG+AGG | 0.552022 | 8.3:+15611174 | None:intergenic |
| GTTTCACTTCAGAATTGTAA+GGG | 0.558550 | 8.3:-15610961 | MS.gene58407:CDS |
| GCTATTATCGAAGAGAATGA+AGG | 0.569442 | 8.3:+15611332 | None:intergenic |
| ATTGCATATCACAAGTGCAT+TGG | 0.569840 | 8.3:+15611127 | None:intergenic |
| CTTGTTCTATCTGGTATGGA+AGG | 0.576428 | 8.3:-15611098 | MS.gene58407:CDS |
| AGAATCATGCATTGGTCAGG+AGG | 0.582085 | 8.3:-15610808 | MS.gene58407:CDS |
| GATAAGGGGAAAGACATCAA+TGG | 0.595318 | 8.3:-15611036 | MS.gene58407:CDS |
| TTGAACAATAGAGAATAGGA+AGG | 0.596055 | 8.3:+15611491 | None:intergenic |
| AAGACAGGAGAATCATGCAT+TGG | 0.601882 | 8.3:-15610816 | MS.gene58407:CDS |
| AAAGCTTGTATCTTGATAAG+GGG | 0.603714 | 8.3:-15611050 | MS.gene58407:CDS |
| TACAATTCTGAAGTGAAACA+AGG | 0.613545 | 8.3:+15610965 | None:intergenic |
| ATCACATTGTTGGAAAGGCA+AGG | 0.615036 | 8.3:-15610935 | MS.gene58407:CDS |
| GGCAAAAGGGATGAAGGAGA+AGG | 0.617930 | 8.3:+15611560 | None:intergenic |
| AACAGTTGCAGATATAGGTG+TGG | 0.621021 | 8.3:+15611291 | None:intergenic |
| AGCAACTCACAATTGTGTCA+AGG | 0.625192 | 8.3:+15610856 | None:intergenic |
| TGATTGTTTGAGAAACCTTG+AGG | 0.628286 | 8.3:+15611459 | None:intergenic |
| CGGTCGCTGACATGAAGAGG+AGG | 0.647162 | 8.3:+15611177 | None:intergenic |
| CTATCTGGTATGGAAGGACA+TGG | 0.647711 | 8.3:-15611092 | MS.gene58407:CDS |
| ATTATCGAAGAGAATGAAGG+TGG | 0.661031 | 8.3:+15611335 | None:intergenic |
| TCTGGTATGGAAGGACATGG+TGG | 0.680756 | 8.3:-15611089 | MS.gene58407:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTCTTTTTGTATTATGTTTT+TGG | - | chr8.3:15611189-15611208 | MS.gene58407:CDS | 15.0% |
| !!! | AAGTGAAATAATGATAGTTT+TGG | + | chr8.3:15610790-15610809 | None:intergenic | 20.0% |
| ! | AATGTTGAACAATAGAGAAT+AGG | + | chr8.3:15610948-15610967 | None:intergenic | 25.0% |
| ! | CTCAAATTTATAACCGTTTA+AGG | - | chr8.3:15610894-15610913 | MS.gene58407:CDS | 25.0% |
| ! | CTTAAACGGTTATAAATTTG+AGG | + | chr8.3:15610896-15610915 | None:intergenic | 25.0% |
| ! | TAAAGCTTGTATCTTGATAA+GGG | - | chr8.3:15611381-15611400 | MS.gene58407:CDS | 25.0% |
| ! | TTAAAGCTTGTATCTTGATA+AGG | - | chr8.3:15611380-15611399 | MS.gene58407:CDS | 25.0% |
| !! | GTTATAAATTTGAGGCAAAA+GGG | + | chr8.3:15610888-15610907 | None:intergenic | 25.0% |
| AAAGCTTGTATCTTGATAAG+GGG | - | chr8.3:15611382-15611401 | MS.gene58407:CDS | 30.0% | |
| CATCAAAAAGAGTCCTTAAA+CGG | + | chr8.3:15610910-15610929 | None:intergenic | 30.0% | |
| GAGTTTAAGTGCTTGAATTA+TGG | + | chr8.3:15611056-15611075 | None:intergenic | 30.0% | |
| GTTTCACTTCAGAATTGTAA+GGG | - | chr8.3:15611471-15611490 | MS.gene58407:CDS | 30.0% | |
| TGTTTCACTTCAGAATTGTA+AGG | - | chr8.3:15611470-15611489 | MS.gene58407:CDS | 30.0% | |
| TTGAACAATAGAGAATAGGA+AGG | + | chr8.3:15610944-15610963 | None:intergenic | 30.0% | |
| TTTCAAGAAGTGGACAATTA+TGG | - | chr8.3:15611655-15611674 | MS.gene58407:CDS | 30.0% | |
| ! | AATATGTCACTTGTTCTATC+TGG | - | chr8.3:15611325-15611344 | MS.gene58407:CDS | 30.0% |
| ! | GGTTATAAATTTGAGGCAAA+AGG | + | chr8.3:15610889-15610908 | None:intergenic | 30.0% |
| ! | TTTTACAAAAGCTTCAAGAC+AGG | - | chr8.3:15611601-15611620 | MS.gene58407:CDS | 30.0% |
| !! | TACAATTCTGAAGTGAAACA+AGG | + | chr8.3:15611470-15611489 | None:intergenic | 30.0% |
| ACTTCCTGTTAATATTGCCT+TGG | - | chr8.3:15611432-15611451 | MS.gene58407:CDS | 35.0% | |
| ATTATCGAAGAGAATGAAGG+TGG | + | chr8.3:15611100-15611119 | None:intergenic | 35.0% | |
| ATTGCATATCACAAGTGCAT+TGG | + | chr8.3:15611308-15611327 | None:intergenic | 35.0% | |
| CAAGAAACAGTTGCAGATAT+AGG | + | chr8.3:15611149-15611168 | None:intergenic | 35.0% | |
| GCTATTATCGAAGAGAATGA+AGG | + | chr8.3:15611103-15611122 | None:intergenic | 35.0% | |
| GGAATATCACATTGTTGGAA+AGG | - | chr8.3:15611492-15611511 | MS.gene58407:CDS | 35.0% | |
| GTAAGGGAATATCACATTGT+TGG | - | chr8.3:15611487-15611506 | MS.gene58407:CDS | 35.0% | |
| GTGGACAATTATGGTTACAT+TGG | - | chr8.3:15611664-15611683 | MS.gene58407:CDS | 35.0% | |
| GTTCAACATTCTTAACCTCA+AGG | - | chr8.3:15610958-15610977 | MS.gene58407:CDS | 35.0% | |
| TGATTGTTTGAGAAACCTTG+AGG | + | chr8.3:15610976-15610995 | None:intergenic | 35.0% | |
| !! | GGACAAACATTTTCAAGAAG+TGG | - | chr8.3:15611645-15611664 | MS.gene58407:CDS | 35.0% |
| !! | TTCAGCTTTGTTGAAACACT+TGG | - | chr8.3:15611214-15611233 | MS.gene58407:CDS | 35.0% |
| AACAGTTGCAGATATAGGTG+TGG | + | chr8.3:15611144-15611163 | None:intergenic | 40.0% | |
| AAGACAGGAGAATCATGCAT+TGG | - | chr8.3:15611616-15611635 | MS.gene58407:CDS | 40.0% | |
| AGAAGGACAGTTAGTGTGAA+AGG | + | chr8.3:15611019-15611038 | None:intergenic | 40.0% | |
| AGCAACTCACAATTGTGTCA+AGG | + | chr8.3:15611579-15611598 | None:intergenic | 40.0% | |
| ATCACATTGTTGGAAAGGCA+AGG | - | chr8.3:15611497-15611516 | MS.gene58407:CDS | 40.0% | |
| CTTGTTCTATCTGGTATGGA+AGG | - | chr8.3:15611334-15611353 | MS.gene58407:CDS | 40.0% | |
| GAAGGACAGTTAGTGTGAAA+GGG | + | chr8.3:15611018-15611037 | None:intergenic | 40.0% | |
| GATAAGGGGAAAGACATCAA+TGG | - | chr8.3:15611396-15611415 | MS.gene58407:CDS | 40.0% | |
| GTCACTTGTTCTATCTGGTA+TGG | - | chr8.3:15611330-15611349 | MS.gene58407:CDS | 40.0% | |
| ! | AAGGCCTTTTGTGCTTTTAG+AGG | - | chr8.3:15611516-15611535 | MS.gene58407:CDS | 40.0% |
| ! | AGGCCTTTTGTGCTTTTAGA+GGG | - | chr8.3:15611517-15611536 | MS.gene58407:CDS | 40.0% |
| ! | ATCCCCTCTAAAAGCACAAA+AGG | + | chr8.3:15611523-15611542 | None:intergenic | 40.0% |
| ! | ATTTGAGGCAAAAGGGATGA+AGG | + | chr8.3:15610881-15610900 | None:intergenic | 40.0% |
| ! | TGGATGCTTTTGCAATCAGA+AGG | + | chr8.3:15611036-15611055 | None:intergenic | 40.0% |
| !! | ATGAGTATCGAAGCAGAGAA+AGG | + | chr8.3:15610836-15610855 | None:intergenic | 40.0% |
| CACAGAAAAAAGAACTGCCC+CGG | + | chr8.3:15611278-15611297 | None:intergenic | 45.0% | |
| CAGATATAGGTGTGGAAGAG+TGG | + | chr8.3:15611136-15611155 | None:intergenic | 45.0% | |
| CTATCTGGTATGGAAGGACA+TGG | - | chr8.3:15611340-15611359 | MS.gene58407:CDS | 45.0% | |
| GACATGAAGAGGAGGAAACT+AGG | + | chr8.3:15611250-15611269 | None:intergenic | 45.0% | |
| GAGAATGAAGGTGGCAAAGA+TGG | + | chr8.3:15611091-15611110 | None:intergenic | 45.0% | |
| GCAGCCAAGGCAATATTAAC+AGG | + | chr8.3:15611439-15611458 | None:intergenic | 45.0% | |
| ! | AGAATCATGCATTGGTCAGG+AGG | - | chr8.3:15611624-15611643 | MS.gene58407:CDS | 45.0% |
| ! | AGGACTCTTTTTGATGCTGC+AGG | - | chr8.3:15610914-15610933 | MS.gene58407:CDS | 45.0% |
| ! | AGGAGAATCATGCATTGGTC+AGG | - | chr8.3:15611621-15611640 | MS.gene58407:CDS | 45.0% |
| !! | GGCCTTTTGTGCTTTTAGAG+GGG | - | chr8.3:15611518-15611537 | MS.gene58407:CDS | 45.0% |
| CAAGGCTTCAATAGCAGCCA+AGG | + | chr8.3:15611452-15611471 | None:intergenic | 50.0% | |
| GGCAAAAGGGATGAAGGAGA+AGG | + | chr8.3:15610875-15610894 | None:intergenic | 50.0% | |
| TCTGGTATGGAAGGACATGG+TGG | - | chr8.3:15611343-15611362 | MS.gene58407:CDS | 50.0% | |
| !! | GGAAGGACATGGTGGATACT+CGG | - | chr8.3:15611351-15611370 | MS.gene58407:CDS | 50.0% |
| CTCCTCTTCATGTCAGCGAC+CGG | - | chr8.3:15611256-15611275 | MS.gene58407:CDS | 55.0% | |
| TCCTCTTCATGTCAGCGACC+GGG | - | chr8.3:15611257-15611276 | MS.gene58407:CDS | 55.0% | |
| CCTCTTCATGTCAGCGACCG+GGG | - | chr8.3:15611258-15611277 | MS.gene58407:CDS | 60.0% | |
| CGGTCGCTGACATGAAGAGG+AGG | + | chr8.3:15611258-15611277 | None:intergenic | 60.0% | |
| CCCCGGTCGCTGACATGAAG+AGG | + | chr8.3:15611261-15611280 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 15610740 | 15611714 | 15610740 | ID=MS.gene58407 |
| chr8.3 | mRNA | 15610740 | 15611714 | 15610740 | ID=MS.gene58407.t1;Parent=MS.gene58407 |
| chr8.3 | exon | 15610740 | 15611714 | 15610740 | ID=MS.gene58407.t1.exon1;Parent=MS.gene58407.t1 |
| chr8.3 | CDS | 15610740 | 15611714 | 15610740 | ID=cds.MS.gene58407.t1;Parent=MS.gene58407.t1 |
| Gene Sequence |
| Protein sequence |
