Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58424.t1 | KEH31936.1 | 91.2 | 57 | 5 | 0 | 1 | 57 | 25 | 81 | 2.80E-20 | 107.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58424.t1 | A0A072URG0 | 91.2 | 57 | 5 | 0 | 1 | 57 | 25 | 81 | 2.0e-20 | 107.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049222 | MS.gene58424 | -0.801589 | 8.38E-49 | -1.69E-46 |
| MS.gene050538 | MS.gene58424 | -0.82173 | 3.46E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58424.t1 | MTR_4g108700 | 91.228 | 57 | 5 | 0 | 1 | 57 | 25 | 81 | 7.25e-33 | 107 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 16 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCATCCTCTCATTAATTCT+TGG | 0.236814 | 8.3:-15846233 | None:intergenic |
| CTCTCATTAATTCTTGGCTT+TGG | 0.277804 | 8.3:-15846227 | None:intergenic |
| AATTAATGAGAGGATGGATT+TGG | 0.333806 | 8.3:+15846238 | MS.gene58424:CDS |
| CTCAATGATTATGCACCATC+TGG | 0.362640 | 8.3:+15846263 | MS.gene58424:CDS |
| CCGGAGTAATTAGGTCAACT+TGG | 0.423783 | 8.3:-15846050 | None:intergenic |
| ACACACCATTACGGTGCTCT+TGG | 0.466740 | 8.3:-15846305 | None:intergenic |
| TATGCACCATCTGGGGCAAA+TGG | 0.473723 | 8.3:+15846272 | MS.gene58424:CDS |
| TCAATGATTATGCACCATCT+GGG | 0.530735 | 8.3:+15846264 | MS.gene58424:CDS |
| GCCAAGAATTAATGAGAGGA+TGG | 0.546052 | 8.3:+15846232 | MS.gene58424:CDS |
| TACTCCGGAAAATACCCCTC+AGG | 0.579089 | 8.3:+15846065 | MS.gene58424:CDS |
| ACACTCCAAGAGCACCGTAA+TGG | 0.580515 | 8.3:+15846300 | MS.gene58424:CDS |
| CCAAGTTGACCTAATTACTC+CGG | 0.594810 | 8.3:+15846050 | MS.gene58424:CDS |
| CAAAGCCAAGAATTAATGAG+AGG | 0.628754 | 8.3:+15846228 | MS.gene58424:CDS |
| TGACGACCATTTGCCCCAGA+TGG | 0.633835 | 8.3:-15846278 | None:intergenic |
| CAATGATTATGCACCATCTG+GGG | 0.636327 | 8.3:+15846265 | MS.gene58424:CDS |
| AGTATCAAACTGTACCTGAG+GGG | 0.746962 | 8.3:-15846079 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACAAGATTATATATGTTAC+AGG | + | chr8.3:15846172-15846191 | MS.gene58424:intron | 20.0% |
| TATTGAGAAAAACTTGCACT+TGG | + | chr8.3:15846199-15846218 | MS.gene58424:CDS | 30.0% | |
| ! | AATTAATGAGAGGATGGATT+TGG | + | chr8.3:15846238-15846257 | MS.gene58424:CDS | 30.0% |
| CAAAGCCAAGAATTAATGAG+AGG | + | chr8.3:15846228-15846247 | MS.gene58424:CDS | 35.0% | |
| CTCTCATTAATTCTTGGCTT+TGG | - | chr8.3:15846230-15846249 | None:intergenic | 35.0% | |
| TCAATGATTATGCACCATCT+GGG | + | chr8.3:15846264-15846283 | MS.gene58424:CDS | 35.0% | |
| TCCATCCTCTCATTAATTCT+TGG | - | chr8.3:15846236-15846255 | None:intergenic | 35.0% | |
| AGTATCAAACTGTACCTGAG+GGG | - | chr8.3:15846082-15846101 | None:intergenic | 40.0% | |
| CAATGATTATGCACCATCTG+GGG | + | chr8.3:15846265-15846284 | MS.gene58424:CDS | 40.0% | |
| CTCAATGATTATGCACCATC+TGG | + | chr8.3:15846263-15846282 | MS.gene58424:CDS | 40.0% | |
| GAGTATCAAACTGTACCTGA+GGG | - | chr8.3:15846083-15846102 | None:intergenic | 40.0% | |
| GCCAAGAATTAATGAGAGGA+TGG | + | chr8.3:15846232-15846251 | MS.gene58424:CDS | 40.0% | |
| ! | AGAGTATCAAACTGTACCTG+AGG | - | chr8.3:15846084-15846103 | None:intergenic | 40.0% |
| ! | CCAAGTTGACCTAATTACTC+CGG | + | chr8.3:15846050-15846069 | MS.gene58424:CDS | 40.0% |
| ! | GGGTATTTTCCGGAGTAATT+AGG | - | chr8.3:15846062-15846081 | None:intergenic | 40.0% |
| CCGGAGTAATTAGGTCAACT+TGG | - | chr8.3:15846053-15846072 | None:intergenic | 45.0% | |
| !!! | TGTACCTGAGGGGTATTTTC+CGG | - | chr8.3:15846072-15846091 | None:intergenic | 45.0% |
| TACTCCGGAAAATACCCCTC+AGG | + | chr8.3:15846065-15846084 | MS.gene58424:CDS | 50.0% | |
| ! | TATGCACCATCTGGGGCAAA+TGG | + | chr8.3:15846272-15846291 | MS.gene58424:CDS | 50.0% |
| TGACGACCATTTGCCCCAGA+TGG | - | chr8.3:15846281-15846300 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 15846039 | 15846319 | 15846039 | ID=MS.gene58424 |
| chr8.3 | mRNA | 15846039 | 15846319 | 15846039 | ID=MS.gene58424.t1;Parent=MS.gene58424 |
| chr8.3 | exon | 15846039 | 15846086 | 15846039 | ID=MS.gene58424.t1.exon1;Parent=MS.gene58424.t1 |
| chr8.3 | CDS | 15846039 | 15846086 | 15846039 | ID=cds.MS.gene58424.t1;Parent=MS.gene58424.t1 |
| chr8.3 | exon | 15846194 | 15846319 | 15846194 | ID=MS.gene58424.t1.exon2;Parent=MS.gene58424.t1 |
| chr8.3 | CDS | 15846194 | 15846319 | 15846194 | ID=cds.MS.gene58424.t1;Parent=MS.gene58424.t1 |
| Gene Sequence |
| Protein sequence |
