Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58502.t1 | XP_003609669.1 | 94.9 | 256 | 11 | 2 | 1 | 254 | 1 | 256 | 2.00E-134 | 488.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58502.t1 | Q9FXC7 | 28.5 | 239 | 158 | 7 | 16 | 245 | 14 | 248 | 1.3e-17 | 91.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58502.t1 | G7JLH1 | 94.9 | 256 | 11 | 2 | 1 | 254 | 1 | 256 | 1.4e-134 | 488.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053931 | MS.gene58502 | 0.817396 | 3.37E-52 | -1.69E-46 |
| MS.gene056834 | MS.gene58502 | 0.80682 | 6.83E-50 | -1.69E-46 |
| MS.gene057208 | MS.gene58502 | 0.811263 | 7.64E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58502.t1 | MTR_4g119850 | 94.922 | 256 | 11 | 2 | 1 | 254 | 1 | 256 | 1.01e-179 | 494 |
| MS.gene58502.t1 | MTR_5g033770 | 77.734 | 256 | 53 | 3 | 1 | 254 | 1 | 254 | 1.87e-142 | 399 |
| MS.gene58502.t1 | MTR_3g075360 | 68.750 | 256 | 72 | 3 | 1 | 254 | 1 | 250 | 3.64e-122 | 348 |
| MS.gene58502.t1 | MTR_4g123430 | 81.319 | 182 | 15 | 1 | 1 | 182 | 1 | 163 | 9.11e-102 | 293 |
| MS.gene58502.t1 | MTR_3g069260 | 69.136 | 81 | 24 | 1 | 32 | 112 | 3 | 82 | 4.33e-34 | 118 |
| MS.gene58502.t1 | MTR_3g069240 | 65.333 | 75 | 26 | 0 | 45 | 119 | 1 | 75 | 4.72e-28 | 102 |
| MS.gene58502.t1 | MTR_4g112590 | 30.242 | 248 | 109 | 10 | 12 | 254 | 17 | 205 | 2.98e-21 | 89.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58502.t1 | AT1G74875 | 31.515 | 165 | 104 | 4 | 29 | 188 | 16 | 176 | 3.75e-20 | 86.7 |
| MS.gene58502.t1 | AT1G67623 | 28.452 | 239 | 158 | 7 | 16 | 245 | 14 | 248 | 7.35e-20 | 87.0 |
| MS.gene58502.t1 | AT2G35280 | 34.058 | 138 | 88 | 2 | 18 | 155 | 4 | 138 | 9.40e-15 | 70.5 |
Find 46 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAACACTCAACATGGTTTA+TGG | 0.219594 | 8.4:-12087006 | MS.gene58502:CDS |
| AAGCATAGAGTAAACATATT+TGG | 0.220285 | 8.4:+12087250 | None:intergenic |
| TTCACCACCTTCTCTGTCTC+AGG | 0.276907 | 8.4:+12087029 | None:intergenic |
| AAACTTGGGTTTGATCTCTT+TGG | 0.278212 | 8.4:-12087194 | MS.gene58502:CDS |
| CGTTTATCAACATGCAACTA+TGG | 0.338892 | 8.4:-12087462 | MS.gene58502:CDS |
| AACTCTTCCAAACGATTTGT+TGG | 0.361021 | 8.4:-12087582 | MS.gene58502:CDS |
| GATTTCTTTCTTGACTCTTA+AGG | 0.371595 | 8.4:+12087652 | None:intergenic |
| TTACGATGCTTATGGGTGTT+TGG | 0.388503 | 8.4:+12087606 | None:intergenic |
| ATATTAACCAACAAATCGTT+TGG | 0.413344 | 8.4:+12087575 | None:intergenic |
| GCAGTATTTCAGTACTTTGA+TGG | 0.417476 | 8.4:-12087327 | MS.gene58502:CDS |
| GATGAAGAAGGAAGAAAACT+TGG | 0.438079 | 8.4:-12087209 | MS.gene58502:CDS |
| CCTTCTCTGTCTCAGGGTTG+TGG | 0.443318 | 8.4:+12087036 | None:intergenic |
| TCCACAGGCATTACTATTGT+GGG | 0.445364 | 8.4:-12087158 | MS.gene58502:CDS |
| TTGCATTGGTTCCACTTCCA+TGG | 0.446150 | 8.4:-12087433 | MS.gene58502:CDS |
| TTTCTTAATGCTTCAAAAGA+CGG | 0.474300 | 8.4:-12087488 | MS.gene58502:CDS |
| TCACCACCTTCTCTGTCTCA+GGG | 0.476807 | 8.4:+12087030 | None:intergenic |
| AACTATGGATAAGTTTGCAT+TGG | 0.477921 | 8.4:-12087447 | MS.gene58502:CDS |
| TTCCACAGGCATTACTATTG+TGG | 0.481179 | 8.4:-12087159 | MS.gene58502:CDS |
| TTGTAAAGAAAGCGGTAACT+TGG | 0.481445 | 8.4:-12087375 | MS.gene58502:CDS |
| CGATTTGTTGGTTAATATCG+TGG | 0.497934 | 8.4:-12087570 | MS.gene58502:CDS |
| TCATCGGTGAACCATGGAAG+TGG | 0.502356 | 8.4:+12087422 | None:intergenic |
| CTATTGTGGGTTGTAGAAAG+AGG | 0.503016 | 8.4:-12087145 | MS.gene58502:CDS |
| GTTGGTTAATATCGTGGGAA+AGG | 0.510068 | 8.4:-12087564 | MS.gene58502:CDS |
| TTGGAGATTATTTATCGTGA+AGG | 0.526959 | 8.4:-12087356 | MS.gene58502:CDS |
| AGTACTTTGATGGTTGATGT+TGG | 0.529050 | 8.4:-12087317 | MS.gene58502:CDS |
| AAAGGTTGCATCACGTTCCA+TGG | 0.529793 | 8.4:-12087546 | MS.gene58502:CDS |
| TATTGTGGGTTGTAGAAAGA+GGG | 0.532034 | 8.4:-12087144 | MS.gene58502:CDS |
| GTTGTGGTGCCTCAAAATCA+AGG | 0.536571 | 8.4:-12087086 | MS.gene58502:CDS |
| GAATTTGATAATGATGATGT+TGG | 0.545329 | 8.4:-12086981 | MS.gene58502:CDS |
| GGTGAGTTGAAGAATTCCAC+AGG | 0.548279 | 8.4:-12087173 | MS.gene58502:CDS |
| GATTTGTTGGTTAATATCGT+GGG | 0.550612 | 8.4:-12087569 | MS.gene58502:CDS |
| ATGAAGAAGGAAGAAAACTT+GGG | 0.555650 | 8.4:-12087208 | MS.gene58502:CDS |
| GCTAACTGGGATGATGAAGA+AGG | 0.571368 | 8.4:-12087221 | MS.gene58502:CDS |
| TGGTTTGAAGAATTTGAAGA+AGG | 0.573775 | 8.4:-12087297 | MS.gene58502:CDS |
| GATTATTTATCGTGAAGGAA+TGG | 0.575748 | 8.4:-12087351 | MS.gene58502:CDS |
| TTTGAAGAAGGCTGCACTAG+AGG | 0.578023 | 8.4:-12087285 | MS.gene58502:CDS |
| TTGAAGAAGGCTGCACTAGA+GGG | 0.579675 | 8.4:-12087284 | MS.gene58502:CDS |
| TTGAGCCGTTGTAAAGAAAG+CGG | 0.605024 | 8.4:-12087383 | MS.gene58502:CDS |
| AGTTACCGCTTTCTTTACAA+CGG | 0.624471 | 8.4:+12087378 | None:intergenic |
| CAACCCTGAGACAGAGAAGG+TGG | 0.639230 | 8.4:-12087033 | MS.gene58502:CDS |
| TTTACAACGGCTCAAGAACG+AGG | 0.653563 | 8.4:+12087391 | None:intergenic |
| CCACAACCCTGAGACAGAGA+AGG | 0.656673 | 8.4:-12087036 | MS.gene58502:CDS |
| ACCCACAATAGTAATGCCTG+TGG | 0.667035 | 8.4:+12087157 | None:intergenic |
| GGTGTTTGCAAATATTGCGA+CGG | 0.668808 | 8.4:-12086960 | MS.gene58502:CDS |
| TGGTGAAGAAACACTCAACA+TGG | 0.674981 | 8.4:-12087013 | MS.gene58502:CDS |
| CATGTGGGTGAAGAATGTTG+TGG | 0.839774 | 8.4:-12087102 | MS.gene58502:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAGCATAGAGTAAACATATT+TGG | + | chr8.4:12087329-12087348 | None:intergenic | 25.0% |
| ! | ATATTAACCAACAAATCGTT+TGG | + | chr8.4:12087004-12087023 | None:intergenic | 25.0% |
| ! | TTTCTTAATGCTTCAAAAGA+CGG | - | chr8.4:12087088-12087107 | MS.gene58502:CDS | 25.0% |
| !!! | GAATTTGATAATGATGATGT+TGG | - | chr8.4:12087595-12087614 | MS.gene58502:CDS | 25.0% |
| !!! | GTTTGTTTTGTAAGATGTTT+CGG | - | chr8.4:12087653-12087672 | MS.gene58502:CDS | 25.0% |
| !!! | TTTGTTTTGTAAGATGTTTC+GGG | - | chr8.4:12087654-12087673 | MS.gene58502:CDS | 25.0% |
| AACTATGGATAAGTTTGCAT+TGG | - | chr8.4:12087129-12087148 | MS.gene58502:CDS | 30.0% | |
| ATGAAGAAGGAAGAAAACTT+GGG | - | chr8.4:12087368-12087387 | MS.gene58502:CDS | 30.0% | |
| GATTATTTATCGTGAAGGAA+TGG | - | chr8.4:12087225-12087244 | MS.gene58502:CDS | 30.0% | |
| GATTTCTTTCTTGACTCTTA+AGG | + | chr8.4:12086927-12086946 | None:intergenic | 30.0% | |
| TGGTTTGAAGAATTTGAAGA+AGG | - | chr8.4:12087279-12087298 | MS.gene58502:CDS | 30.0% | |
| TTGGAGATTATTTATCGTGA+AGG | - | chr8.4:12087220-12087239 | MS.gene58502:CDS | 30.0% | |
| ! | AAGAGTTTTTACGATGCTTA+TGG | + | chr8.4:12086981-12087000 | None:intergenic | 30.0% |
| ! | AGAGTTTTTACGATGCTTAT+GGG | + | chr8.4:12086980-12086999 | None:intergenic | 30.0% |
| ! | AGTCTTTTATTCAAAGCATG+TGG | - | chr8.4:12087458-12087477 | MS.gene58502:CDS | 30.0% |
| ! | GTCTTTTATTCAAAGCATGT+GGG | - | chr8.4:12087459-12087478 | MS.gene58502:CDS | 30.0% |
| ! | TGTTTACTCTATGCTTTTGA+TGG | - | chr8.4:12087333-12087352 | MS.gene58502:CDS | 30.0% |
| !! | GATTTGTTGGTTAATATCGT+GGG | - | chr8.4:12087007-12087026 | MS.gene58502:CDS | 30.0% |
| AAACTTGGGTTTGATCTCTT+TGG | - | chr8.4:12087382-12087401 | MS.gene58502:CDS | 35.0% | |
| AACTCTTCCAAACGATTTGT+TGG | - | chr8.4:12086994-12087013 | MS.gene58502:CDS | 35.0% | |
| AGTTACCGCTTTCTTTACAA+CGG | + | chr8.4:12087201-12087220 | None:intergenic | 35.0% | |
| CGTTTATCAACATGCAACTA+TGG | - | chr8.4:12087114-12087133 | MS.gene58502:CDS | 35.0% | |
| GAAACACTCAACATGGTTTA+TGG | - | chr8.4:12087570-12087589 | MS.gene58502:CDS | 35.0% | |
| GATGAAGAAGGAAGAAAACT+TGG | - | chr8.4:12087367-12087386 | MS.gene58502:CDS | 35.0% | |
| GCAGTATTTCAGTACTTTGA+TGG | - | chr8.4:12087249-12087268 | MS.gene58502:CDS | 35.0% | |
| TTGTAAAGAAAGCGGTAACT+TGG | - | chr8.4:12087201-12087220 | MS.gene58502:CDS | 35.0% | |
| ! | AAAGAGAGTCCTTGATTTTG+AGG | + | chr8.4:12087502-12087521 | None:intergenic | 35.0% |
| ! | AGTACTTTGATGGTTGATGT+TGG | - | chr8.4:12087259-12087278 | MS.gene58502:CDS | 35.0% |
| ! | TATTGTGGGTTGTAGAAAGA+GGG | - | chr8.4:12087432-12087451 | MS.gene58502:CDS | 35.0% |
| !! | CGATTTGTTGGTTAATATCG+TGG | - | chr8.4:12087006-12087025 | MS.gene58502:CDS | 35.0% |
| !! | GAACGAGGTTTCTTTTTCAT+CGG | + | chr8.4:12087173-12087192 | None:intergenic | 35.0% |
| !! | TATGCTTTTGATGGCTAACT+GGG | - | chr8.4:12087342-12087361 | MS.gene58502:CDS | 35.0% |
| !!! | TTATGGGTGTTTGGTTTTTG+CGG | + | chr8.4:12086964-12086983 | None:intergenic | 35.0% |
| GGTGTTTGCAAATATTGCGA+CGG | - | chr8.4:12087616-12087635 | MS.gene58502:CDS | 40.0% | |
| GTTGGTTAATATCGTGGGAA+AGG | - | chr8.4:12087012-12087031 | MS.gene58502:CDS | 40.0% | |
| TCCACAGGCATTACTATTGT+GGG | - | chr8.4:12087418-12087437 | MS.gene58502:CDS | 40.0% | |
| TGGTGAAGAAACACTCAACA+TGG | - | chr8.4:12087563-12087582 | MS.gene58502:CDS | 40.0% | |
| TTACGATGCTTATGGGTGTT+TGG | + | chr8.4:12086973-12086992 | None:intergenic | 40.0% | |
| TTCCACAGGCATTACTATTG+TGG | - | chr8.4:12087417-12087436 | MS.gene58502:CDS | 40.0% | |
| TTGAGCCGTTGTAAAGAAAG+CGG | - | chr8.4:12087193-12087212 | MS.gene58502:CDS | 40.0% | |
| ! | CTATTGTGGGTTGTAGAAAG+AGG | - | chr8.4:12087431-12087450 | MS.gene58502:CDS | 40.0% |
| ! | GATTTTGCAAAGATCAGCCA+TGG | + | chr8.4:12087050-12087069 | None:intergenic | 40.0% |
| !! | CTATGCTTTTGATGGCTAAC+TGG | - | chr8.4:12087341-12087360 | MS.gene58502:CDS | 40.0% |
| !! | TCTTTTTCATCGGTGAACCA+TGG | + | chr8.4:12087163-12087182 | None:intergenic | 40.0% |
| !!! | GTTTGGTTTTTGCGGCTTTT+AGG | + | chr8.4:12086956-12086975 | None:intergenic | 40.0% |
| AAAGGTTGCATCACGTTCCA+TGG | - | chr8.4:12087030-12087049 | MS.gene58502:CDS | 45.0% | |
| ACCCACAATAGTAATGCCTG+TGG | + | chr8.4:12087422-12087441 | None:intergenic | 45.0% | |
| CATGTGGGTGAAGAATGTTG+TGG | - | chr8.4:12087474-12087493 | MS.gene58502:CDS | 45.0% | |
| GCTAACTGGGATGATGAAGA+AGG | - | chr8.4:12087355-12087374 | MS.gene58502:CDS | 45.0% | |
| GTTGTGGTGCCTCAAAATCA+AGG | - | chr8.4:12087490-12087509 | MS.gene58502:CDS | 45.0% | |
| TTGAAGAAGGCTGCACTAGA+GGG | - | chr8.4:12087292-12087311 | MS.gene58502:CDS | 45.0% | |
| TTTACAACGGCTCAAGAACG+AGG | + | chr8.4:12087188-12087207 | None:intergenic | 45.0% | |
| TTTGAAGAAGGCTGCACTAG+AGG | - | chr8.4:12087291-12087310 | MS.gene58502:CDS | 45.0% | |
| ! | GGTGAGTTGAAGAATTCCAC+AGG | - | chr8.4:12087403-12087422 | MS.gene58502:CDS | 45.0% |
| !! | TTGCATTGGTTCCACTTCCA+TGG | - | chr8.4:12087143-12087162 | MS.gene58502:CDS | 45.0% |
| TCACCACCTTCTCTGTCTCA+GGG | + | chr8.4:12087549-12087568 | None:intergenic | 50.0% | |
| TCATCGGTGAACCATGGAAG+TGG | + | chr8.4:12087157-12087176 | None:intergenic | 50.0% | |
| TTCACCACCTTCTCTGTCTC+AGG | + | chr8.4:12087550-12087569 | None:intergenic | 50.0% | |
| CAACCCTGAGACAGAGAAGG+TGG | - | chr8.4:12087543-12087562 | MS.gene58502:CDS | 55.0% | |
| CCACAACCCTGAGACAGAGA+AGG | - | chr8.4:12087540-12087559 | MS.gene58502:CDS | 55.0% | |
| CCTTCTCTGTCTCAGGGTTG+TGG | + | chr8.4:12087543-12087562 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 12086917 | 12087681 | 12086917 | ID=MS.gene58502 |
| chr8.4 | mRNA | 12086917 | 12087681 | 12086917 | ID=MS.gene58502.t1;Parent=MS.gene58502 |
| chr8.4 | exon | 12086917 | 12087681 | 12086917 | ID=MS.gene58502.t1.exon1;Parent=MS.gene58502.t1 |
| chr8.4 | CDS | 12086917 | 12087681 | 12086917 | ID=cds.MS.gene58502.t1;Parent=MS.gene58502.t1 |
| Gene Sequence |
| Protein sequence |
