AlfalfaGEDB | MS.gene58516

M. sativa cultivar XinJiangDaYe / MS.gene58516

KEGG: K03671
GO: GO:0003674 GO:0003824 GO:0006662 GO:0008150 GO:0008152 GO:0009987 GO:0015035 GO:0015036 GO:0016491 GO:0016667 GO:0018904 GO:0019725 GO:0042592 GO:0044281 GO:0045454 GO:0050789 GO:0050794 GO:0065007 GO:0065008 GO:0071704
PFAM: PF00085.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58516.t1	ACZ37071.1	100	127	0	0	1	127	1	127	3.90E-62	247.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58516.t1	Q38879	52.3	111	53	0	16	126	22	132	3.9e-31	135.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58516.t1	D8L5E2	100.0	127	0	0	1	127	1	127	2.8e-62	247.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene057606	MS.gene58516	0.970297	2.10E-131	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58516.t1	MTR_5g037950	99.213	127	1	0	1	127	1	127	9.54e-91	258
MS.gene58516.t1	MTR_5g037930	72.441	127	34	1	1	126	1	127	1.45e-54	167
MS.gene58516.t1	MTR_5g037890	66.667	126	41	1	1	126	1	125	6.50e-53	162
MS.gene58516.t1	MTR_7g009070	60.577	104	41	0	23	126	30	133	7.25e-45	142
MS.gene58516.t1	MTR_3g112410	49.550	111	56	0	15	125	2	112	4.79e-38	125
MS.gene58516.t1	MTR_1g023140	43.750	112	63	0	15	126	2	113	1.37e-34	116
MS.gene58516.t1	MTR_5g021180	44.144	111	62	0	15	125	4	114	6.89e-34	114
MS.gene58516.t1	MTR_8g107460	43.137	102	58	0	21	122	256	357	2.50e-27	103
MS.gene58516.t1	MTR_4g081380	44.340	106	57	2	19	123	10	114	7.97e-26	94.4
MS.gene58516.t1	MTR_2g010750	40.952	105	62	0	19	123	9	113	2.39e-25	93.2
MS.gene58516.t1	MTR_2g082590	39.604	101	59	1	23	123	29	127	2.24e-23	88.6
MS.gene58516.t1	MTR_2g082590	35.238	105	68	0	19	123	10	114	3.36e-23	87.4
MS.gene58516.t1	MTR_8g005320	38.710	93	57	0	30	122	37	129	4.66e-23	87.8
MS.gene58516.t1	MTR_7g073680	39.560	91	55	0	30	120	35	125	4.82e-23	87.8
MS.gene58516.t1	MTR_8g006685	38.710	93	57	0	30	122	106	198	1.95e-22	87.8
MS.gene58516.t1	MTR_8g006560	38.710	93	57	0	30	122	106	198	1.95e-22	87.8
MS.gene58516.t1	MTR_1g098660	38.372	86	50	2	40	124	77	160	2.58e-19	79.0
MS.gene58516.t1	MTR_4g094690	39.024	82	48	1	45	124	85	166	9.95e-19	77.4
MS.gene58516.t1	MTR_7g080250	41.379	87	50	1	41	126	94	180	5.42e-16	70.5
MS.gene58516.t1	MTR_3g089065	37.349	83	50	2	44	124	89	171	4.50e-15	68.2
MS.gene58516.t1	MTR_2g079420	38.554	83	49	2	44	124	89	171	4.01e-14	65.5
MS.gene58516.t1	MTR_8g059015	36.145	83	51	2	44	124	92	174	2.04e-13	63.5
MS.gene58516.t1	MTR_1g114290	34.615	78	47	2	40	116	85	159	7.07e-13	62.4
MS.gene58516.t1	MTR_4g088905	32.500	80	54	0	45	124	25	104	1.22e-11	60.8
MS.gene58516.t1	MTR_4g081380	36.905	84	51	2	13	95	4	86	1.38e-11	57.0
MS.gene58516.t1	MTR_4g085880	31.579	76	51	1	44	118	84	159	6.77e-11	57.0
MS.gene58516.t1	MTR_2g079340	36.620	71	44	1	43	112	54	124	8.72e-11	56.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58516.t1	AT5G39950	52.252	111	53	0	16	126	22	132	1.79e-44	142
MS.gene58516.t1	AT1G59730	43.077	130	70	2	1	127	1	129	1.16e-37	124
MS.gene58516.t1	AT3G51030	44.144	111	62	0	15	125	2	112	6.05e-34	114
MS.gene58516.t1	AT5G42980	48.113	106	55	0	21	126	7	112	3.14e-33	112
MS.gene58516.t1	AT1G69880	48.077	104	54	0	21	124	39	142	2.72e-32	111
MS.gene58516.t1	AT1G19730	45.536	112	60	1	15	125	2	113	4.15e-31	107
MS.gene58516.t1	AT1G45145	41.905	105	61	0	21	125	7	111	2.03e-30	105
MS.gene58516.t1	AT3G17880	39.474	114	65	1	13	122	253	366	1.54e-25	99.0
MS.gene58516.t1	AT3G17880	39.474	114	65	1	13	122	260	373	1.61e-25	99.4
MS.gene58516.t1	AT1G11530	39.806	103	62	0	21	123	4	106	7.44e-24	89.4
MS.gene58516.t1	AT3G08710	41.758	91	53	0	30	120	34	124	2.45e-23	88.6
MS.gene58516.t1	AT3G08710	41.758	91	53	0	30	120	34	124	2.45e-23	88.6
MS.gene58516.t1	AT3G08710	41.758	91	53	0	30	120	34	124	2.45e-23	88.6
MS.gene58516.t1	AT3G56420	38.043	92	57	0	29	120	50	141	4.44e-23	88.2
MS.gene58516.t1	AT3G56420	38.043	92	57	0	29	120	50	141	4.44e-23	88.2
MS.gene58516.t1	AT3G56420	38.043	92	57	0	29	120	50	141	4.44e-23	88.2
MS.gene58516.t1	AT2G40790	39.130	92	56	0	29	120	50	141	1.72e-19	79.0
MS.gene58516.t1	AT2G40790	39.130	92	56	0	29	120	50	141	1.72e-19	79.0
MS.gene58516.t1	AT1G43560	41.379	87	49	2	40	124	75	161	4.76e-19	78.2
MS.gene58516.t1	AT1G76760	38.372	86	51	2	40	123	80	165	2.28e-18	76.6
MS.gene58516.t1	AT5G16400	42.529	87	49	1	41	126	97	183	1.71e-17	74.7
MS.gene58516.t1	AT2G35010	40.244	82	47	1	45	124	110	191	6.68e-17	73.2
MS.gene58516.t1	AT2G35010	40.244	82	47	1	45	124	110	191	6.68e-17	73.2
MS.gene58516.t1	AT3G02730	43.023	86	48	1	42	126	88	173	8.91e-17	72.4
MS.gene58516.t1	AT3G15360	32.673	101	63	3	26	124	92	189	6.05e-15	68.2
MS.gene58516.t1	AT1G31020	39.024	82	48	1	45	124	75	156	7.98e-15	67.0
MS.gene58516.t1	AT1G03680	38.272	81	48	2	46	124	97	177	1.43e-14	67.0
MS.gene58516.t1	AT4G03520	33.735	83	53	2	44	124	101	183	1.13e-13	64.7
MS.gene58516.t1	AT1G52990	34.066	91	58	2	35	124	222	311	1.73e-13	65.9
MS.gene58516.t1	AT1G50320	39.189	74	41	2	44	116	90	160	3.80e-13	63.2
MS.gene58516.t1	AT4G04950	29.787	94	66	0	31	124	11	104	9.42e-12	61.2
MS.gene58516.t1	AT4G32580	30.864	81	56	0	44	124	24	104	9.86e-12	58.9
MS.gene58516.t1	AT1G07700	36.364	99	55	2	34	124	100	198	1.72e-11	59.3
MS.gene58516.t1	AT1G07700	36.364	99	55	2	34	124	100	198	1.72e-11	59.3
MS.gene58516.t1	AT1G07700	36.364	99	55	2	34	124	100	198	1.72e-11	59.3
MS.gene58516.t1	AT1G07700	36.364	99	55	2	34	124	113	211	3.61e-11	58.5

Find 25 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCAGCTAATTCTTTGATAAT+TGG	0.122091	5.3:+26111476	None:intergenic
GGAGGTGGCAAGTGCATTTC+AGG	0.225705	5.3:-26111307	MS.gene58516:CDS
CTCAACAACAGACTCTAAAT+TGG	0.230357	5.3:+26112202	None:intergenic
GATTGACGTGGATGAGTTAA+TGG	0.268688	5.3:-26111426	MS.gene58516:intron
CCTTCCACTCAACTGCTAAA+TGG	0.287365	5.3:-26112151	MS.gene58516:CDS
CAGCTAATTCTTTGATAATT+GGG	0.312442	5.3:+26111477	None:intergenic
TACCAGCTTGTTTGTTACTT+TGG	0.383274	5.3:+26112109	None:intergenic
AGACTCTAAATTGGAAAAGT+TGG	0.412160	5.3:+26112211	None:intergenic
AAAGATGGTCATTGATTTCA+CGG	0.422756	5.3:-26111531	MS.gene58516:intron
TAAATGGAAGGCTCACTTTG+AGG	0.488391	5.3:-26112135	MS.gene58516:CDS
TCATTGATTTCACGGCTACT+TGG	0.488401	5.3:-26111523	MS.gene58516:CDS
CCATTTAGCAGTTGAGTGGA+AGG	0.494217	5.3:+26112151	None:intergenic
TTCTTCAATAGTATAAATGT+TGG	0.495545	5.3:+26111273	None:intergenic
GATTTCACGGCTACTTGGTG+TGG	0.515795	5.3:-26111518	MS.gene58516:CDS
ACATTTATACTATTGAAGAA+AGG	0.532669	5.3:-26111270	MS.gene58516:CDS
GTGTGGACCTTGCAAATACA+TGG	0.544731	5.3:-26111501	MS.gene58516:CDS
TTCCAAAGTAACAAACAAGC+TGG	0.568571	5.3:-26112111	MS.gene58516:intron
GAAACTGATTGAGAAACGTA+GGG	0.577131	5.3:-26111202	MS.gene58516:CDS
CCTTCCATTTAGCAGTTGAG+TGG	0.582577	5.3:+26112147	None:intergenic
AGAAACTGATTGAGAAACGT+AGG	0.583938	5.3:-26111203	MS.gene58516:CDS
ATTGGGTCCATGTATTTGCA+AGG	0.586486	5.3:+26111494	None:intergenic
CCACTCAACTGCTAAATGGA+AGG	0.588835	5.3:-26112147	MS.gene58516:CDS
TAGCAGTTGAGTGGAAGGTG+AGG	0.659322	5.3:+26112156	None:intergenic
TGAGTTCATTAAGATTGACG+TGG	0.684888	5.3:-26111438	MS.gene58516:CDS
TGGAAGGTGAGGATGAGCGA+TGG	0.701264	5.3:+26112167	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTCTTAATTATTTTTAAAGA+TGG	-	chr5.3:26111869-26111888	MS.gene58516:intron	10.0%
!!	TATAGATATAAGATATCAAA+AGG	+	chr5.3:26112046-26112065	None:intergenic	15.0%
!!!	ATGTGAAATAATTTTTTTAG+AGG	-	chr5.3:26111453-26111472	MS.gene58516:CDS	15.0%
!!!	GCTAAAATTTTCTTATTATT+TGG	-	chr5.3:26111809-26111828	MS.gene58516:intron	15.0%
!!!	GTTTTCATTTTTTTTTTTGT+TGG	+	chr5.3:26111536-26111555	None:intergenic	15.0%
!!!	TGTGAAATAATTTTTTTAGA+GGG	-	chr5.3:26111454-26111473	MS.gene58516:CDS	15.0%
!!!	TTGTTGGTAAATTAATTATA+TGG	+	chr5.3:26111520-26111539	None:intergenic	15.0%
!!	ACATTTATACTATTGAAGAA+AGG	-	chr5.3:26112145-26112164	MS.gene58516:CDS	20.0%
!!	GATATCAAAAGGTAATTAAA+AGG	+	chr5.3:26112035-26112054	None:intergenic	20.0%
!!	TTCTTCAATAGTATAAATGT+TGG	+	chr5.3:26112145-26112164	None:intergenic	20.0%
!!!	AATAAGAAAATTTTAGCCAA+AGG	+	chr5.3:26111806-26111825	None:intergenic	20.0%
!!!	TTAAAATCGTTTGTTTTATG+AGG	-	chr5.3:26111833-26111852	MS.gene58516:intron	20.0%
!!!	TTTTTAGATACTTTGAAGTT+TGG	+	chr5.3:26111493-26111512	None:intergenic	20.0%
!	AAAACCAAGGTTTCAAAAAA+CGG	-	chr5.3:26111618-26111637	MS.gene58516:intron	25.0%
!	ATGACAACAATATCAATCAT+GGG	+	chr5.3:26111661-26111680	None:intergenic	25.0%
!!	AAATTTTGTGACGAGAAAAA+CGG	+	chr5.3:26111564-26111583	None:intergenic	25.0%
!!	CAGCTAATTCTTTGATAATT+GGG	+	chr5.3:26111941-26111960	None:intergenic	25.0%
!!!	TTTTTCATCGGTTAAAATCT+TGG	+	chr5.3:26111601-26111620	None:intergenic	25.0%
	AAAAGTAGTTGAAAAAGTGG+TGG	-	chr5.3:26112168-26112187	MS.gene58516:CDS	30.0%
	AAAGTAGTTGAAAAAGTGGT+GGG	-	chr5.3:26112169-26112188	MS.gene58516:CDS	30.0%
	AGACTCTAAATTGGAAAAGT+TGG	+	chr5.3:26111207-26111226	None:intergenic	30.0%
	AGGAAAAGTAGTTGAAAAAG+TGG	-	chr5.3:26112165-26112184	MS.gene58516:CDS	30.0%
	CATGACAACAATATCAATCA+TGG	+	chr5.3:26111662-26111681	None:intergenic	30.0%
	GATAATGCAAAATGTACCAA+TGG	-	chr5.3:26112080-26112099	MS.gene58516:intron	30.0%
	TAACATTGAATGATTCGATG+AGG	+	chr5.3:26111398-26111417	None:intergenic	30.0%
	TTTAACCGATGAAAAAACCA+AGG	-	chr5.3:26111605-26111624	MS.gene58516:intron	30.0%
!	GCAGCTAATTCTTTGATAAT+TGG	+	chr5.3:26111942-26111961	None:intergenic	30.0%
!!	AAAGATGGTCATTGATTTCA+CGG	-	chr5.3:26111884-26111903	MS.gene58516:intron	30.0%
!!!	AAATCGTTTGTTTTATGAGG+TGG	-	chr5.3:26111836-26111855	MS.gene58516:intron	30.0%
!!!	TAGCCAAAGGTTTTAAATCA+CGG	+	chr5.3:26111793-26111812	None:intergenic	30.0%
!!!	TGAAACCTTGGTTTTTTCAT+CGG	+	chr5.3:26111613-26111632	None:intergenic	30.0%
	AACGATACTACGTAGTAGTT+TGG	-	chr5.3:26111359-26111378	MS.gene58516:intron	35.0%
	AGAAACTGATTGAGAAACGT+AGG	-	chr5.3:26112212-26112231	MS.gene58516:CDS	35.0%
	CAAAATGTACCAATGGAAAC+AGG	-	chr5.3:26112087-26112106	MS.gene58516:intron	35.0%
	CTCAACAACAGACTCTAAAT+TGG	+	chr5.3:26111216-26111235	None:intergenic	35.0%
	GAAACTGATTGAGAAACGTA+GGG	-	chr5.3:26112213-26112232	MS.gene58516:CDS	35.0%
	GTTAAAATCTTGGTTGTGCA+TGG	+	chr5.3:26111591-26111610	None:intergenic	35.0%
	TGAGTTCATTAAGATTGACG+TGG	-	chr5.3:26111977-26111996	MS.gene58516:intron	35.0%
	TTCCAAAGTAACAAACAAGC+TGG	-	chr5.3:26111304-26111323	MS.gene58516:CDS	35.0%
!	TACCAGCTTGTTTGTTACTT+TGG	+	chr5.3:26111309-26111328	None:intergenic	35.0%
	AAACGGCCCATGATTGATAT+CGG	-	chr5.3:26111635-26111654	MS.gene58516:intron	40.0%
	AATGTACCAATGGAAACAGG+AGG	-	chr5.3:26112090-26112109	MS.gene58516:intron	40.0%
	ATTGGGTCCATGTATTTGCA+AGG	+	chr5.3:26111924-26111943	None:intergenic	40.0%
	GATTGACGTGGATGAGTTAA+TGG	-	chr5.3:26111989-26112008	MS.gene58516:intron	40.0%
	TAAATGGAAGGCTCACTTTG+AGG	-	chr5.3:26111280-26111299	MS.gene58516:CDS	40.0%
!	AAAAGTGGTGGGAGCAAAAA+AGG	-	chr5.3:26112180-26112199	MS.gene58516:CDS	40.0%
!	CGACCGTGATTTAAAACCTT+TGG	-	chr5.3:26111787-26111806	MS.gene58516:intron	40.0%
!!	TCATTGATTTCACGGCTACT+TGG	-	chr5.3:26111892-26111911	MS.gene58516:intron	40.0%
!!!	AAGGTTTTAAATCACGGTCG+TGG	+	chr5.3:26111787-26111806	None:intergenic	40.0%
	CATGGGCCGATATCAATCAT+GGG	+	chr5.3:26111644-26111663	None:intergenic	45.0%
	CCACTCAACTGCTAAATGGA+AGG	-	chr5.3:26111268-26111287	MS.gene58516:CDS	45.0%
	CCATTTAGCAGTTGAGTGGA+AGG	+	chr5.3:26111267-26111286	None:intergenic	45.0%
	CCTTCCACTCAACTGCTAAA+TGG	-	chr5.3:26111264-26111283	MS.gene58516:CDS	45.0%
	CCTTCCATTTAGCAGTTGAG+TGG	+	chr5.3:26111271-26111290	None:intergenic	45.0%
	GTGTGGACCTTGCAAATACA+TGG	-	chr5.3:26111914-26111933	MS.gene58516:intron	45.0%
	TCATGGGCCGATATCAATCA+TGG	+	chr5.3:26111645-26111664	None:intergenic	45.0%
!!!	TGGGCCGTTTTTTGAAACCT+TGG	+	chr5.3:26111625-26111644	None:intergenic	45.0%
	GTACCAATGGAAACAGGAGG+TGG	-	chr5.3:26112093-26112112	MS.gene58516:intron	50.0%
	TAGCAGTTGAGTGGAAGGTG+AGG	+	chr5.3:26111262-26111281	None:intergenic	50.0%
	TTGCCACCTCCTGTTTCCAT+TGG	+	chr5.3:26112099-26112118	None:intergenic	50.0%
!	GATTTCACGGCTACTTGGTG+TGG	-	chr5.3:26111897-26111916	MS.gene58516:intron	50.0%
	GGAGGTGGCAAGTGCATTTC+AGG	-	chr5.3:26112108-26112127	MS.gene58516:intron	55.0%
	TGGAAGGTGAGGATGAGCGA+TGG	+	chr5.3:26111251-26111270	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.3	gene	26111197	26112240	26111197	ID=MS.gene58516
chr5.3	mRNA	26111197	26112240	26111197	ID=MS.gene58516.t1;Parent=MS.gene58516
chr5.3	exon	26112112	26112240	26112112	ID=MS.gene58516.t1.exon1;Parent=MS.gene58516.t1
chr5.3	CDS	26112112	26112240	26112112	ID=cds.MS.gene58516.t1;Parent=MS.gene58516.t1
chr5.3	exon	26111427	26111549	26111427	ID=MS.gene58516.t1.exon2;Parent=MS.gene58516.t1
chr5.3	CDS	26111427	26111549	26111427	ID=cds.MS.gene58516.t1;Parent=MS.gene58516.t1
chr5.3	exon	26111197	26111328	26111197	ID=MS.gene58516.t1.exon3;Parent=MS.gene58516.t1
chr5.3	CDS	26111197	26111328	26111197	ID=cds.MS.gene58516.t1;Parent=MS.gene58516.t1

Gene Sequence

>MS.gene58516

ATGGGAGCCAACTTTTCCAATTTAGAGTCTGTTGTTGAGAAATCATCACAGCCATCGCTCATCCTCACCTTCCACTCAACTGCTAAATGGAAGGCTCACTTTGAGGCTTCCAAAGTAACAAACAAGCTGGTATAAACTTCTGATATATAATTAGATCAACACAACGATACTACGTAGTAGTTTGGTTGAATATATACTCCTCATCGAATCATTCAATGTTATCTTATATTTTATATTAATTTATTTTAAACTTCAAATGTGAAATAATTTTTTTAGAGGGTATCAGTATTCAACCAAACTTCAAAGTATCTAAAAATTTACCATATAATTAATTTACCAACAAAAAAAAAAATGAAAACTTTCTCCGTTTTTCTCGTCACAAAATTTAGTGCCATGCACAACCAAGATTTTAACCGATGAAAAAACCAAGGTTTCAAAAAACGGCCCATGATTGATATCGGCCCATGATTGATATTGTTGTCATGAAGTCAAGTTTCTCAACATTTTCTTTGCATTGCGTTTGTCATTGCGACTATATGACATATATTTGATCACAATTTGTTGCAATATCAAAATTCACGATGCAACCACGACCGTGATTTAAAACCTTTGGCTAAAATTTTCTTATTATTTGGCTTAAAATCGTTTGTTTTATGAGGTGGTTAATTTTGTTTCTTAATTATTTTTAAAGATGGTCATTGATTTCACGGCTACTTGGTGTGGACCTTGCAAATACATGGACCCAATTATCAAAGAATTAGCTGCAAAATACAAAGATGTTGAGTTCATTAAGATTGACGTGGATGAGTTAATGGTATGATACATATGATTGATTCCTTTTAATTACCTTTTGATATCTTATATCTATATATATAAACAATATGATAATGCAAAATGTACCAATGGAAACAGGAGGTGGCAAGTGCATTTCAGGTGCAAGCAATGCCAACATTTATACTATTGAAGAAAGGAAAAGTAGTTGAAAAAGTGGTGGGAGCAAAAAAGGAGCAATTACAGAAACTGATTGAGAAACGTAGGGTTTGA

Protein sequence

>MS.gene58516.t1

MGANFSNLESVVEKSSQPSLILTFHSTAKWKAHFEASKVTNKLMVIDFTATWCGPCKYMDPIIKELAAKYKDVEFIKIDVDELMEVASAFQVQAMPTFILLKKGKVVEKVVGAKKEQLQKLIEKRRV