AlfalfaGEDB | MS.gene58517

M. sativa cultivar XinJiangDaYe / MS.gene58517

KEGG: K03671
GO: GO:0003674 GO:0003824 GO:0006662 GO:0008150 GO:0008152 GO:0009987 GO:0015035 GO:0015036 GO:0016491 GO:0016667 GO:0018904 GO:0019725 GO:0042592 GO:0044281 GO:0045454 GO:0050789 GO:0050794 GO:0065007 GO:0065008 GO:0071704
PFAM: PF00085.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58517.t1	XP_003613548.1	98.4	128	2	0	1	128	1	128	2.40E-64	254.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58517.t1	Q38879	46.9	130	64	1	1	125	1	130	2.8e-29	129.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58517.t1	D8L5E1	98.4	128	2	0	1	128	1	128	1.8e-64	254.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene057607	MS.gene58517	0.901821	1.82E-78	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58517.t1	MTR_5g037930	98.438	128	2	0	1	128	1	128	1.19e-92	263
MS.gene58517.t1	MTR_5g037950	73.228	127	33	1	1	127	1	126	1.07e-54	167
MS.gene58517.t1	MTR_5g037890	68.750	128	38	1	1	128	1	126	6.07e-54	165
MS.gene58517.t1	MTR_7g009070	59.615	104	42	0	24	127	30	133	1.95e-44	142
MS.gene58517.t1	MTR_3g112410	47.664	107	56	0	20	126	6	112	8.35e-37	122
MS.gene58517.t1	MTR_1g023140	46.296	108	58	0	21	128	7	114	3.04e-36	120
MS.gene58517.t1	MTR_5g021180	42.478	113	65	0	14	126	2	114	9.17e-35	117
MS.gene58517.t1	MTR_8g107460	41.509	106	62	0	21	126	255	360	6.38e-28	105
MS.gene58517.t1	MTR_2g010750	38.095	105	65	0	20	124	9	113	2.04e-23	88.2
MS.gene58517.t1	MTR_7g073680	40.659	91	54	0	31	121	35	125	5.22e-22	85.1
MS.gene58517.t1	MTR_8g005320	35.238	105	68	0	19	123	25	129	7.88e-22	84.7
MS.gene58517.t1	MTR_8g006685	38.710	93	57	0	31	123	106	198	2.85e-21	85.1
MS.gene58517.t1	MTR_8g006560	38.710	93	57	0	31	123	106	198	2.85e-21	85.1
MS.gene58517.t1	MTR_2g082590	32.381	105	71	0	20	124	10	114	5.88e-21	82.0
MS.gene58517.t1	MTR_4g081380	37.273	110	67	2	20	128	10	118	1.36e-20	80.9
MS.gene58517.t1	MTR_2g082590	37.209	86	54	0	39	124	42	127	6.80e-19	77.0
MS.gene58517.t1	MTR_4g094690	36.585	82	50	1	46	125	85	166	2.26e-16	71.2
MS.gene58517.t1	MTR_1g098660	31.250	96	63	2	31	125	67	160	2.39e-16	71.2
MS.gene58517.t1	MTR_2g079420	37.500	88	53	2	40	125	84	171	2.31e-14	66.2
MS.gene58517.t1	MTR_8g059015	35.227	88	55	2	40	125	87	174	6.32e-14	65.1
MS.gene58517.t1	MTR_3g089065	32.143	84	55	2	45	126	89	172	3.05e-13	63.2
MS.gene58517.t1	MTR_7g080250	35.443	79	50	1	42	119	94	172	3.94e-13	63.2
MS.gene58517.t1	MTR_1g114290	32.911	79	49	2	40	117	84	159	5.56e-12	60.1
MS.gene58517.t1	MTR_4g085880	34.211	76	49	1	45	119	84	159	7.63e-12	59.3
MS.gene58517.t1	MTR_3g112400	30.928	97	47	3	20	116	5	81	2.87e-11	55.8
MS.gene58517.t1	MTR_2g079340	38.028	71	43	1	44	113	54	124	9.16e-11	56.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58517.t1	AT5G39950	46.923	130	64	1	1	125	1	130	1.32e-42	137
MS.gene58517.t1	AT1G59730	42.969	128	72	1	1	127	1	128	4.13e-38	125
MS.gene58517.t1	AT1G69880	49.074	108	55	0	19	126	36	143	1.86e-35	119
MS.gene58517.t1	AT5G42980	47.222	108	57	0	21	128	6	113	1.42e-34	116
MS.gene58517.t1	AT1G19730	49.533	107	53	1	21	126	7	113	1.78e-34	116
MS.gene58517.t1	AT3G51030	44.340	106	59	0	21	126	7	112	6.08e-34	114
MS.gene58517.t1	AT1G45145	44.860	107	59	0	20	126	5	111	7.70e-34	114
MS.gene58517.t1	AT3G17880	40.000	105	63	0	22	126	265	369	3.12e-25	98.2
MS.gene58517.t1	AT3G17880	39.623	106	64	0	21	126	271	376	3.96e-25	98.2
MS.gene58517.t1	AT3G56420	39.130	92	56	0	30	121	50	141	1.36e-23	89.7
MS.gene58517.t1	AT3G56420	39.130	92	56	0	30	121	50	141	1.36e-23	89.7
MS.gene58517.t1	AT3G56420	39.130	92	56	0	30	121	50	141	1.36e-23	89.7
MS.gene58517.t1	AT1G11530	34.286	105	69	0	20	124	2	106	8.93e-21	81.3
MS.gene58517.t1	AT2G40790	39.130	92	56	0	30	121	50	141	1.18e-19	79.3
MS.gene58517.t1	AT2G40790	39.130	92	56	0	30	121	50	141	1.18e-19	79.3
MS.gene58517.t1	AT3G08710	34.951	103	65	1	21	123	26	126	1.23e-19	79.0
MS.gene58517.t1	AT3G08710	34.951	103	65	1	21	123	26	126	1.23e-19	79.0
MS.gene58517.t1	AT3G08710	34.951	103	65	1	21	123	26	126	1.23e-19	79.0
MS.gene58517.t1	AT3G15360	37.363	91	49	3	40	125	102	189	4.36e-16	71.2
MS.gene58517.t1	AT1G43560	30.159	126	79	5	9	128	39	161	9.50e-16	69.7
MS.gene58517.t1	AT2G35010	38.554	83	47	2	46	125	110	191	1.64e-14	67.0
MS.gene58517.t1	AT2G35010	38.554	83	47	2	46	125	110	191	1.64e-14	67.0
MS.gene58517.t1	AT1G76760	33.333	75	49	1	41	114	80	154	2.20e-14	66.2
MS.gene58517.t1	AT4G03520	34.940	83	52	2	45	125	101	183	8.21e-14	65.1
MS.gene58517.t1	AT1G31020	30.556	108	73	1	20	125	49	156	9.70e-14	64.3
MS.gene58517.t1	AT1G03680	35.802	81	50	2	47	125	97	177	2.34e-13	63.5
MS.gene58517.t1	AT3G02730	35.632	87	55	1	43	128	88	174	2.87e-13	63.5
MS.gene58517.t1	AT5G16400	34.091	88	57	1	42	128	97	184	3.94e-13	63.2
MS.gene58517.t1	AT1G52990	31.522	92	61	2	36	126	222	312	7.63e-12	61.2
MS.gene58517.t1	AT4G04950	29.787	94	66	0	32	125	11	104	2.51e-11	60.1
MS.gene58517.t1	AT4G32580	27.660	94	68	0	32	125	11	104	7.73e-11	56.6
MS.gene58517.t1	AT2G15570	25.843	89	65	1	32	119	74	162	8.66e-11	56.6
MS.gene58517.t1	AT2G15570	25.843	89	65	1	32	119	75	163	9.36e-11	56.6

Find 23 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CACCAGCTTGTTAGTTTCTT+TGG	0.210380	5.3:+26070197	None:intergenic
CCTTCCATTCCACTGCTAAA+TGG	0.240816	5.3:-26070239	MS.gene58517:CDS
TAAGATGGTAATTGAATTTA+CGG	0.284078	5.3:-26070080	MS.gene58517:intron
GATTGACGTGGATGAATTAA+TGG	0.293121	5.3:-26069975	MS.gene58517:intron
GCAGCAAAGTCTTGAATGAT+TGG	0.331944	5.3:+26070025	None:intergenic
AAAGTTGTTGACAAGGTTGT+AGG	0.419387	5.3:-26069396	MS.gene58517:CDS
CAGCTTGTTAGTTTCTTTGG+AGG	0.429788	5.3:+26070200	None:intergenic
TTCTTCATCAGTATGAATGT+TGG	0.495900	5.3:+26069423	None:intergenic
CCTTCCATTTAGCAGTGGAA+TGG	0.530623	5.3:+26070235	None:intergenic
CTCCAAAGAAACTAACAAGC+TGG	0.546462	5.3:-26070199	MS.gene58517:intron
GTGTGGACCTTGCAAATACA+TGG	0.552168	5.3:-26070050	MS.gene58517:CDS
CCATTTAGCAGTGGAATGGA+AGG	0.552428	5.3:+26070239	None:intergenic
CCATTCCACTGCTAAATGGA+AGG	0.553763	5.3:-26070235	MS.gene58517:CDS
ACATTCATACTGATGAAGAA+AGG	0.574346	5.3:-26069420	MS.gene58517:CDS
ATTGGATCCATGTATTTGCA+AGG	0.586486	5.3:+26070043	None:intergenic
GAATTTACGGCTGCATGGTG+TGG	0.600755	5.3:-26070067	MS.gene58517:CDS
GAAAGGAAAAGTTGTTGACA+AGG	0.605147	5.3:-26069403	MS.gene58517:CDS
GGGAGGCAATTTATCCAACA+TGG	0.609050	5.3:-26070307	MS.gene58517:CDS
AATTTATCCAACATGGAGCA+TGG	0.613422	5.3:-26070300	MS.gene58517:CDS
GTAAGCCTTCCATTTAGCAG+TGG	0.620491	5.3:+26070230	None:intergenic
TGTATGACCATGCTCCATGT+TGG	0.633501	5.3:+26070293	None:intergenic
AGATTTCATCAAGATTGACG+TGG	0.662014	5.3:-26069987	MS.gene58517:CDS
TAATTGAATTTACGGCTGCA+TGG	0.665704	5.3:-26070072	MS.gene58517:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AACTTAACAGTATTAATAAA+GGG	-	chr5.3:26069995-26070014	MS.gene58517:CDS	15.0%
!!	ATATTATATCTCCTAATATA+AGG	-	chr5.3:26069745-26069764	MS.gene58517:intron	15.0%
!!	ATCTTTATATAATGTATAGA+TGG	+	chr5.3:26069779-26069798	None:intergenic	15.0%
!!	GAAATGAGATTTATATTTAA+AGG	-	chr5.3:26070056-26070075	MS.gene58517:CDS	15.0%
!!	TATTATATCTCCTAATATAA+GGG	-	chr5.3:26069746-26069765	MS.gene58517:intron	15.0%
!!!	AAGGTGATTTTTTTTTTTAA+AGG	-	chr5.3:26070075-26070094	MS.gene58517:CDS	15.0%
!!!	AGGTGATTTTTTTTTTTAAA+GGG	-	chr5.3:26070076-26070095	MS.gene58517:CDS	15.0%
!!!	ATTATTTTTCAATTGTAAAC+AGG	-	chr5.3:26070178-26070197	MS.gene58517:intron	15.0%
!!!	TTATTTTTCAATTGTAAACA+GGG	-	chr5.3:26070179-26070198	MS.gene58517:intron	15.0%
!!	CAACTTAACAGTATTAATAA+AGG	-	chr5.3:26069994-26070013	MS.gene58517:CDS	20.0%
!!	TAAGATGGTAATTGAATTTA+CGG	-	chr5.3:26069576-26069595	MS.gene58517:intron	20.0%
!!!	AATACTGTTAAGTTGAATTA+AGG	+	chr5.3:26069989-26070008	None:intergenic	20.0%
!!!	ATACTGTTAAGTTGAATTAA+GGG	+	chr5.3:26069988-26070007	None:intergenic	20.0%
!!!	ATTTGAAATGAGACTTTTTT+AGG	-	chr5.3:26070140-26070159	MS.gene58517:intron	20.0%
!!!	GGTGATTTTTTTTTTTAAAG+GGG	-	chr5.3:26070077-26070096	MS.gene58517:CDS	20.0%
!!!	TATTTTTTTTTGGCTTAAGA+TGG	-	chr5.3:26069561-26069580	MS.gene58517:intron	20.0%
!!!	TTTGTTTCTTTAACTCTAAT+GGG	-	chr5.3:26069532-26069551	MS.gene58517:intron	20.0%
!!!	TTTTGTTTCTTTAACTCTAA+TGG	-	chr5.3:26069531-26069550	MS.gene58517:intron	20.0%
!!	GTATTTTATGTGATTTCCTA+TGG	-	chr5.3:26069862-26069881	MS.gene58517:intron	25.0%
	ACATTCATACTGATGAAGAA+AGG	-	chr5.3:26070236-26070255	MS.gene58517:CDS	30.0%
	GAATTAAGGGATATAAAAGC+CGG	+	chr5.3:26069975-26069994	None:intergenic	30.0%
	TTCTTCATCAGTATGAATGT+TGG	+	chr5.3:26070236-26070255	None:intergenic	30.0%
!	TACACGCAAATGAGTATTTT+AGG	+	chr5.3:26069939-26069958	None:intergenic	30.0%
!!!	TGGGATCGTGTATTTTTTTT+TGG	-	chr5.3:26069551-26069570	MS.gene58517:intron	30.0%
	AAAGTTGTTGACAAGGTTGT+AGG	-	chr5.3:26070260-26070279	MS.gene58517:CDS	35.0%
	AATTTATCCAACATGGAGCA+TGG	-	chr5.3:26069356-26069375	MS.gene58517:CDS	35.0%
	AGATTTCATCAAGATTGACG+TGG	-	chr5.3:26069669-26069688	MS.gene58517:intron	35.0%
	ATTGGATCCATGTATTTGCA+AGG	+	chr5.3:26069616-26069635	None:intergenic	35.0%
	CGTGTAAATGTATCAAACTC+CGG	-	chr5.3:26069953-26069972	MS.gene58517:intron	35.0%
	GAAAGGAAAAGTTGTTGACA+AGG	-	chr5.3:26070253-26070272	MS.gene58517:CDS	35.0%
	GATTGACGTGGATGAATTAA+TGG	-	chr5.3:26069681-26069700	MS.gene58517:intron	35.0%
	TAATTGAATTTACGGCTGCA+TGG	-	chr5.3:26069584-26069603	MS.gene58517:intron	35.0%
	TTCGTATGGAAAAAGACCAT+AGG	+	chr5.3:26069881-26069900	None:intergenic	35.0%
!	TCTTAATCATCCGATTCGTA+TGG	+	chr5.3:26069895-26069914	None:intergenic	35.0%
!	TGGTCTTTTTCCATACGAAT+CGG	-	chr5.3:26069882-26069901	MS.gene58517:intron	35.0%
	CAAGGTTGTAGGAGCAAAAA+AGG	-	chr5.3:26070271-26070290	MS.gene58517:CDS	40.0%
	CTCCAAAGAAACTAACAAGC+TGG	-	chr5.3:26069457-26069476	MS.gene58517:CDS	40.0%
	GCAGCAAAGTCTTGAATGAT+TGG	+	chr5.3:26069634-26069653	None:intergenic	40.0%
	TGGTGTGTTGCCCTTATATT+AGG	+	chr5.3:26069759-26069778	None:intergenic	40.0%
!!	CACCAGCTTGTTAGTTTCTT+TGG	+	chr5.3:26069462-26069481	None:intergenic	40.0%
!!	CAGCTTGTTAGTTTCTTTGG+AGG	+	chr5.3:26069459-26069478	None:intergenic	40.0%
	CCATTCCACTGCTAAATGGA+AGG	-	chr5.3:26069421-26069440	MS.gene58517:CDS	45.0%
	CCATTTAGCAGTGGAATGGA+AGG	+	chr5.3:26069420-26069439	None:intergenic	45.0%
	CCTTCCATTCCACTGCTAAA+TGG	-	chr5.3:26069417-26069436	MS.gene58517:CDS	45.0%
	CCTTCCATTTAGCAGTGGAA+TGG	+	chr5.3:26069424-26069443	None:intergenic	45.0%
	GGGAGGCAATTTATCCAACA+TGG	-	chr5.3:26069349-26069368	MS.gene58517:CDS	45.0%
	GTAAGCCTTCCATTTAGCAG+TGG	+	chr5.3:26069429-26069448	None:intergenic	45.0%
	GTGTGGACCTTGCAAATACA+TGG	-	chr5.3:26069606-26069625	MS.gene58517:intron	45.0%
	TGTATGACCATGCTCCATGT+TGG	+	chr5.3:26069366-26069385	None:intergenic	45.0%
	GAATTTACGGCTGCATGGTG+TGG	-	chr5.3:26069589-26069608	MS.gene58517:intron	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.3	gene	26069347	26070331	26069347	ID=MS.gene58517
chr5.3	mRNA	26069347	26070331	26069347	ID=MS.gene58517.t1;Parent=MS.gene58517
chr5.3	exon	26070200	26070331	26070200	ID=MS.gene58517.t1.exon1;Parent=MS.gene58517.t1
chr5.3	CDS	26070200	26070331	26070200	ID=cds.MS.gene58517.t1;Parent=MS.gene58517.t1
chr5.3	exon	26069976	26070098	26069976	ID=MS.gene58517.t1.exon2;Parent=MS.gene58517.t1
chr5.3	CDS	26069976	26070098	26069976	ID=cds.MS.gene58517.t1;Parent=MS.gene58517.t1
chr5.3	exon	26069347	26069478	26069347	ID=MS.gene58517.t1.exon3;Parent=MS.gene58517.t1
chr5.3	CDS	26069347	26069478	26069347	ID=cds.MS.gene58517.t1;Parent=MS.gene58517.t1

Gene Sequence

>MS.gene58517

ATGGGAGGCAATTTATCCAACATGGAGCATGGTCATACATCAGCAAAGTCATTATCATCTCACATTCTTACCTTCCATTCCACTGCTAAATGGAAGGCTTACTTTGATGCCTCCAAAGAAACTAACAAGCTGGTGCGTTATTATTAATTTAATTTAAAGCTATACTAATTATTTTGTATTTTCATTTTGTTTCTTTAACTCTAATGGGATCGTGTATTTTTTTTTGGCTTAAGATGGTAATTGAATTTACGGCTGCATGGTGTGGACCTTGCAAATACATGGATCCAATCATTCAAGACTTTGCTGCAAAATACATAAAAGTAGATTTCATCAAGATTGACGTGGATGAATTAATGGTATTATTACATTTTTATTTCATTTTACTATTATTATTACTCATATTATATCTCCTAATATAAGGGCAACACACCATCTATACATTATATAAAGATGAATAACATATTACTTATAAGCTCTTTTTATTATTTTTACAAATATTATGTAAAACAAATAGTGTATTTTATGTGATTTCCTATGGTCTTTTTCCATACGAATCGGATGATTAAGACAAAAAAAAAGTACGAATATTCCTAAAATACTCATTTGCGTGTAAATGTATCAAACTCCGGCTTTTATATCCCTTAATTCAACTTAACAGTATTAATAAAGGGTATATTTGATAGAAAATAATAAATATATTTCATAACTTGAAATGAGATTTATATTTAAAGGTGATTTTTTTTTTTAAAGGGGAGTTATGACTAGAGACAAAATTAGTAATTTTTTTAGCTGCATTTGAAATGAGACTTTTTTAGGAATTGACTTGTGCACATTATTTTTCAATTGTAAACAGGGTGTCTCTCAAGAGTTTCAAGTGCAAGCAATGCCAACATTCATACTGATGAAGAAAGGAAAAGTTGTTGACAAGGTTGTAGGAGCAAAAAAGGAAGAGCTTGAAAAGTTGATTGAGAAGCATCAAAACTAA

Protein sequence

>MS.gene58517.t1

MGGNLSNMEHGHTSAKSLSSHILTFHSTAKWKAYFDASKETNKLMVIEFTAAWCGPCKYMDPIIQDFAAKYIKVDFIKIDVDELMGVSQEFQVQAMPTFILMKKGKVVDKVVGAKKEELEKLIEKHQN