AlfalfaGEDB | MS.gene057607

M. sativa cultivar XinJiangDaYe / MS.gene057607

KEGG: K03671
GO: GO:0003674 GO:0003824 GO:0006662 GO:0008150 GO:0008152 GO:0009987 GO:0015035 GO:0015036 GO:0016491 GO:0016667 GO:0018904 GO:0019725 GO:0042592 GO:0044281 GO:0045454 GO:0050789 GO:0050794 GO:0065007 GO:0065008 GO:0071704
PFAM: PF00085.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057607.t1	XP_003613548.1	98.4	128	2	0	1	128	1	128	2.40E-64	254.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057607.t1	Q38879	46.9	130	64	1	1	125	1	130	2.8e-29	129.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057607.t1	D8L5E1	98.4	128	2	0	1	128	1	128	1.8e-64	254.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene057607	MS.gene26474	0.828515	8.61E-55	-1.69E-46
MS.gene057607	MS.gene26475	0.819798	9.61E-53	-1.69E-46
MS.gene057607	MS.gene49609	0.955679	1.74E-113	-1.69E-46
MS.gene057607	MS.gene58517	0.901821	1.82E-78	-1.69E-46
MS.gene057607	MS.gene63578	0.823148	1.62E-53	-1.69E-46
MS.gene057607	MS.gene76405	0.802991	4.31E-49	-1.69E-46
MS.gene057607	MS.gene76883	0.819566	1.09E-52	-1.69E-46
MS.gene057607	MS.gene84157	0.804616	1.98E-49	-1.69E-46
MS.gene057607	MS.gene90819	0.815353	9.66E-52	-1.69E-46
MS.gene057607	MS.gene96836	0.808237	3.42E-50	-1.69E-46
MS.gene057607	MS.gene97340	0.805128	1.55E-49	-1.69E-46
MS.gene057607	MS.gene99127	0.818604	1.80E-52	-1.69E-46
MS.gene057607	MS.gene99603	0.809386	1.94E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057607.t1	MTR_5g037930	98.438	128	2	0	1	128	1	128	1.19e-92	263
MS.gene057607.t1	MTR_5g037950	73.228	127	33	1	1	127	1	126	1.07e-54	167
MS.gene057607.t1	MTR_5g037890	68.750	128	38	1	1	128	1	126	6.07e-54	165
MS.gene057607.t1	MTR_7g009070	59.615	104	42	0	24	127	30	133	1.95e-44	142
MS.gene057607.t1	MTR_3g112410	47.664	107	56	0	20	126	6	112	8.35e-37	122
MS.gene057607.t1	MTR_1g023140	46.296	108	58	0	21	128	7	114	3.04e-36	120
MS.gene057607.t1	MTR_5g021180	42.478	113	65	0	14	126	2	114	9.17e-35	117
MS.gene057607.t1	MTR_8g107460	41.509	106	62	0	21	126	255	360	6.38e-28	105
MS.gene057607.t1	MTR_2g010750	38.095	105	65	0	20	124	9	113	2.04e-23	88.2
MS.gene057607.t1	MTR_7g073680	40.659	91	54	0	31	121	35	125	5.22e-22	85.1
MS.gene057607.t1	MTR_8g005320	35.238	105	68	0	19	123	25	129	7.88e-22	84.7
MS.gene057607.t1	MTR_8g006685	38.710	93	57	0	31	123	106	198	2.85e-21	85.1
MS.gene057607.t1	MTR_8g006560	38.710	93	57	0	31	123	106	198	2.85e-21	85.1
MS.gene057607.t1	MTR_2g082590	32.381	105	71	0	20	124	10	114	5.88e-21	82.0
MS.gene057607.t1	MTR_4g081380	37.273	110	67	2	20	128	10	118	1.36e-20	80.9
MS.gene057607.t1	MTR_2g082590	37.209	86	54	0	39	124	42	127	6.80e-19	77.0
MS.gene057607.t1	MTR_4g094690	36.585	82	50	1	46	125	85	166	2.26e-16	71.2
MS.gene057607.t1	MTR_1g098660	31.250	96	63	2	31	125	67	160	2.39e-16	71.2
MS.gene057607.t1	MTR_2g079420	37.500	88	53	2	40	125	84	171	2.31e-14	66.2
MS.gene057607.t1	MTR_8g059015	35.227	88	55	2	40	125	87	174	6.32e-14	65.1
MS.gene057607.t1	MTR_3g089065	32.143	84	55	2	45	126	89	172	3.05e-13	63.2
MS.gene057607.t1	MTR_7g080250	35.443	79	50	1	42	119	94	172	3.94e-13	63.2
MS.gene057607.t1	MTR_1g114290	32.911	79	49	2	40	117	84	159	5.56e-12	60.1
MS.gene057607.t1	MTR_4g085880	34.211	76	49	1	45	119	84	159	7.63e-12	59.3
MS.gene057607.t1	MTR_3g112400	30.928	97	47	3	20	116	5	81	2.87e-11	55.8
MS.gene057607.t1	MTR_2g079340	38.028	71	43	1	44	113	54	124	9.16e-11	56.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057607.t1	AT5G39950	46.923	130	64	1	1	125	1	130	1.32e-42	137
MS.gene057607.t1	AT1G59730	42.969	128	72	1	1	127	1	128	4.13e-38	125
MS.gene057607.t1	AT1G69880	49.074	108	55	0	19	126	36	143	1.86e-35	119
MS.gene057607.t1	AT5G42980	47.222	108	57	0	21	128	6	113	1.42e-34	116
MS.gene057607.t1	AT1G19730	49.533	107	53	1	21	126	7	113	1.78e-34	116
MS.gene057607.t1	AT3G51030	44.340	106	59	0	21	126	7	112	6.08e-34	114
MS.gene057607.t1	AT1G45145	44.860	107	59	0	20	126	5	111	7.70e-34	114
MS.gene057607.t1	AT3G17880	40.000	105	63	0	22	126	265	369	3.12e-25	98.2
MS.gene057607.t1	AT3G17880	39.623	106	64	0	21	126	271	376	3.96e-25	98.2
MS.gene057607.t1	AT3G56420	39.130	92	56	0	30	121	50	141	1.36e-23	89.7
MS.gene057607.t1	AT3G56420	39.130	92	56	0	30	121	50	141	1.36e-23	89.7
MS.gene057607.t1	AT3G56420	39.130	92	56	0	30	121	50	141	1.36e-23	89.7
MS.gene057607.t1	AT1G11530	34.286	105	69	0	20	124	2	106	8.93e-21	81.3
MS.gene057607.t1	AT2G40790	39.130	92	56	0	30	121	50	141	1.18e-19	79.3
MS.gene057607.t1	AT2G40790	39.130	92	56	0	30	121	50	141	1.18e-19	79.3
MS.gene057607.t1	AT3G08710	34.951	103	65	1	21	123	26	126	1.23e-19	79.0
MS.gene057607.t1	AT3G08710	34.951	103	65	1	21	123	26	126	1.23e-19	79.0
MS.gene057607.t1	AT3G08710	34.951	103	65	1	21	123	26	126	1.23e-19	79.0
MS.gene057607.t1	AT3G15360	37.363	91	49	3	40	125	102	189	4.36e-16	71.2
MS.gene057607.t1	AT1G43560	30.159	126	79	5	9	128	39	161	9.50e-16	69.7
MS.gene057607.t1	AT2G35010	38.554	83	47	2	46	125	110	191	1.64e-14	67.0
MS.gene057607.t1	AT2G35010	38.554	83	47	2	46	125	110	191	1.64e-14	67.0
MS.gene057607.t1	AT1G76760	33.333	75	49	1	41	114	80	154	2.20e-14	66.2
MS.gene057607.t1	AT4G03520	34.940	83	52	2	45	125	101	183	8.21e-14	65.1
MS.gene057607.t1	AT1G31020	30.556	108	73	1	20	125	49	156	9.70e-14	64.3
MS.gene057607.t1	AT1G03680	35.802	81	50	2	47	125	97	177	2.34e-13	63.5
MS.gene057607.t1	AT3G02730	35.632	87	55	1	43	128	88	174	2.87e-13	63.5
MS.gene057607.t1	AT5G16400	34.091	88	57	1	42	128	97	184	3.94e-13	63.2
MS.gene057607.t1	AT1G52990	31.522	92	61	2	36	126	222	312	7.63e-12	61.2
MS.gene057607.t1	AT4G04950	29.787	94	66	0	32	125	11	104	2.51e-11	60.1
MS.gene057607.t1	AT4G32580	27.660	94	68	0	32	125	11	104	7.73e-11	56.6
MS.gene057607.t1	AT2G15570	25.843	89	65	1	32	119	74	162	8.66e-11	56.6
MS.gene057607.t1	AT2G15570	25.843	89	65	1	32	119	75	163	9.36e-11	56.6

Find 26 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CACCAGCTTGTTAGTTTCTT+TGG	0.210380	5.4:+27210194	None:intergenic
CCTTCCATTCCACTGCTAAA+TGG	0.240816	5.4:-27210236	MS.gene057607:CDS
TAAGATGGTAATTGAATTTA+CGG	0.284078	5.4:-27210079	MS.gene057607:intron
GATTGACGTGGATGAATTAA+TGG	0.293121	5.4:-27209974	MS.gene057607:intron
GCAGCAAAGTCTTGAATGAT+TGG	0.331944	5.4:+27210024	None:intergenic
AAAGTTGTTGACAAGGTTGT+AGG	0.405303	5.4:-27209382	MS.gene057607:CDS
CAGCTTGTTAGTTTCTTTGG+AGG	0.429788	5.4:+27210197	None:intergenic
TTCTTCATCAGTATGAATGT+TGG	0.495900	5.4:+27209409	None:intergenic
AAGTTGTTGACAAGGTTGTA+GGG	0.508376	5.4:-27209381	MS.gene057607:CDS
CATTCATACTGATGAAGAAA+GGG	0.508654	5.4:-27209405	MS.gene057607:CDS
CCTTCCATTTAGCAGTGGAA+TGG	0.530623	5.4:+27210232	None:intergenic
CTCCAAAGAAACTAACAAGC+TGG	0.546462	5.4:-27210196	MS.gene057607:intron
ACATTCATACTGATGAAGAA+AGG	0.550243	5.4:-27209406	MS.gene057607:CDS
GTGTGGACCTTGCAAATACA+TGG	0.552168	5.4:-27210049	MS.gene057607:CDS
CCATTTAGCAGTGGAATGGA+AGG	0.552428	5.4:+27210236	None:intergenic
CCATTCCACTGCTAAATGGA+AGG	0.553763	5.4:-27210232	MS.gene057607:CDS
ATTGGATCCATGTATTTGCA+AGG	0.586486	5.4:+27210042	None:intergenic
GGGAGGCAATTTATCCAACA+TGG	0.596510	5.4:-27210304	MS.gene057607:CDS
GAATTTACGGCTGCATGGTG+TGG	0.600755	5.4:-27210066	MS.gene057607:CDS
GAAAGGGAAAGTTGTTGACA+AGG	0.604559	5.4:-27209389	MS.gene057607:CDS
AATTTATCCAACATGGAGCA+TGG	0.613422	5.4:-27210297	MS.gene057607:CDS
ATAAGCCTTCCATTTAGCAG+TGG	0.613671	5.4:+27210227	None:intergenic
AGTTGTTGACAAGGTTGTAG+GGG	0.619000	5.4:-27209380	MS.gene057607:CDS
TGTATGACCATGCTCCATGT+TGG	0.627275	5.4:+27210290	None:intergenic
AGATTTCATCAAGATTGACG+TGG	0.662014	5.4:-27209986	MS.gene057607:CDS
TAATTGAATTTACGGCTGCA+TGG	0.665704	5.4:-27210071	MS.gene057607:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATACATTAATTATACTCAAA+TGG	+	chr5.4:27209944-27209963	None:intergenic	15.0%
!!	ATCTTTATATAATGTATAGA+TGG	+	chr5.4:27209763-27209782	None:intergenic	15.0%
!!!	AAGATGAACTTTTTTTTTAA+AGG	-	chr5.4:27210073-27210092	MS.gene057607:CDS	15.0%
!!!	AGATGAACTTTTTTTTTAAA+GGG	-	chr5.4:27210074-27210093	MS.gene057607:CDS	15.0%
!!!	ATTATTTTTCAATTGTAAAC+AGG	-	chr5.4:27210175-27210194	MS.gene057607:intron	15.0%
!!!	TTATTTTTCAATTGTAAACA+GGG	-	chr5.4:27210176-27210195	MS.gene057607:intron	15.0%
!!	ATAATTAATGTATCAAACTC+CGG	-	chr5.4:27209951-27209970	MS.gene057607:intron	20.0%
!!	ATGTTATATCTCCTAATATA+AGG	-	chr5.4:27209729-27209748	MS.gene057607:intron	20.0%
!!	TAAGATGGTAATTGAATTTA+CGG	-	chr5.4:27209560-27209579	MS.gene057607:intron	20.0%
!!	TGTTATATCTCCTAATATAA+GGG	-	chr5.4:27209730-27209749	MS.gene057607:intron	20.0%
!!!	AATACTGTTAAGTTGAATTA+AGG	+	chr5.4:27209987-27210006	None:intergenic	20.0%
!!!	ATACTGTTAAGTTGAATTAA+GGG	+	chr5.4:27209986-27210005	None:intergenic	20.0%
!!!	GATGAACTTTTTTTTTAAAG+GGG	-	chr5.4:27210075-27210094	MS.gene057607:CDS	20.0%
!!!	TATACTCAAATGGTTATTTT+AGG	+	chr5.4:27209934-27209953	None:intergenic	20.0%
!!!	TTTGTTTTTTTAACTCTCAT+GGG	-	chr5.4:27209518-27209537	MS.gene057607:intron	20.0%
!!!	TTTTGTTTTTTTAACTCTCA+TGG	-	chr5.4:27209517-27209536	MS.gene057607:intron	20.0%
!	TTACTTATAAGCTCTTACTA+TGG	-	chr5.4:27209853-27209872	MS.gene057607:intron	25.0%
!!!	TGTATTTTTTTGGCTTAAGA+TGG	-	chr5.4:27209545-27209564	MS.gene057607:intron	25.0%
	ACATTCATACTGATGAAGAA+AGG	-	chr5.4:27210233-27210252	MS.gene057607:CDS	30.0%
	CATTCATACTGATGAAGAAA+GGG	-	chr5.4:27210234-27210253	MS.gene057607:CDS	30.0%
	GAATTAAGGGATATAAAAGC+CGG	+	chr5.4:27209973-27209992	None:intergenic	30.0%
	GTTATGACTAGACACAAAAT+TGG	-	chr5.4:27210099-27210118	MS.gene057607:intron	30.0%
	TTCTTCATCAGTATGAATGT+TGG	+	chr5.4:27210233-27210252	None:intergenic	30.0%
!!	ATTTGAAACGAGACTTTTTG+AGG	-	chr5.4:27210137-27210156	MS.gene057607:intron	30.0%
	AAAGTTGTTGACAAGGTTGT+AGG	-	chr5.4:27210257-27210276	MS.gene057607:CDS	35.0%
	AAGTTGTTGACAAGGTTGTA+GGG	-	chr5.4:27210258-27210277	MS.gene057607:CDS	35.0%
	AATTTATCCAACATGGAGCA+TGG	-	chr5.4:27209342-27209361	MS.gene057607:CDS	35.0%
	AGATTTCATCAAGATTGACG+TGG	-	chr5.4:27209653-27209672	MS.gene057607:intron	35.0%
	ATTGGATCCATGTATTTGCA+AGG	+	chr5.4:27209600-27209619	None:intergenic	35.0%
	GATTGACGTGGATGAATTAA+TGG	-	chr5.4:27209665-27209684	MS.gene057607:intron	35.0%
	TAATTGAATTTACGGCTGCA+TGG	-	chr5.4:27209568-27209587	MS.gene057607:intron	35.0%
!!!	CATGGGATCGTGTATTTTTT+TGG	-	chr5.4:27209535-27209554	MS.gene057607:intron	35.0%
	AGTTGTTGACAAGGTTGTAG+GGG	-	chr5.4:27210259-27210278	MS.gene057607:CDS	40.0%
	ATAAGCCTTCCATTTAGCAG+TGG	+	chr5.4:27209415-27209434	None:intergenic	40.0%
	CTCCAAAGAAACTAACAAGC+TGG	-	chr5.4:27209443-27209462	MS.gene057607:CDS	40.0%
	GAAAGGGAAAGTTGTTGACA+AGG	-	chr5.4:27210250-27210269	MS.gene057607:CDS	40.0%
	GCAGCAAAGTCTTGAATGAT+TGG	+	chr5.4:27209618-27209637	None:intergenic	40.0%
!	TGGTCTTTTTCCATACGAAC+AGG	-	chr5.4:27209873-27209892	MS.gene057607:intron	40.0%
!!	CACCAGCTTGTTAGTTTCTT+TGG	+	chr5.4:27209448-27209467	None:intergenic	40.0%
!!	CAGCTTGTTAGTTTCTTTGG+AGG	+	chr5.4:27209445-27209464	None:intergenic	40.0%
	CAAGGTTGTAGGGGCAAAAA+AGG	-	chr5.4:27210268-27210287	MS.gene057607:CDS	45.0%
	CCATACGAACAGGATGACTA+AGG	-	chr5.4:27209883-27209902	MS.gene057607:intron	45.0%
	CCATTCCACTGCTAAATGGA+AGG	-	chr5.4:27209407-27209426	MS.gene057607:CDS	45.0%
	CCATTTAGCAGTGGAATGGA+AGG	+	chr5.4:27209406-27209425	None:intergenic	45.0%
	CCTTAGTCATCCTGTTCGTA+TGG	+	chr5.4:27209886-27209905	None:intergenic	45.0%
	CCTTCCATTCCACTGCTAAA+TGG	-	chr5.4:27209403-27209422	MS.gene057607:CDS	45.0%
	CCTTCCATTTAGCAGTGGAA+TGG	+	chr5.4:27209410-27209429	None:intergenic	45.0%
	GGGAGGCAATTTATCCAACA+TGG	-	chr5.4:27209335-27209354	MS.gene057607:CDS	45.0%
	GTGTGGACCTTGCAAATACA+TGG	-	chr5.4:27209590-27209609	MS.gene057607:intron	45.0%
	TGGTGTGCTGCCCTTATATT+AGG	+	chr5.4:27209743-27209762	None:intergenic	45.0%
	TGTATGACCATGCTCCATGT+TGG	+	chr5.4:27209352-27209371	None:intergenic	45.0%
	GAATTTACGGCTGCATGGTG+TGG	-	chr5.4:27209573-27209592	MS.gene057607:intron	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	27209333	27210328	27209333	ID=MS.gene057607
chr5.4	mRNA	27209333	27210328	27209333	ID=MS.gene057607.t1;Parent=MS.gene057607
chr5.4	exon	27210197	27210328	27210197	ID=MS.gene057607.t1.exon1;Parent=MS.gene057607.t1
chr5.4	CDS	27210197	27210328	27210197	ID=cds.MS.gene057607.t1;Parent=MS.gene057607.t1
chr5.4	exon	27209975	27210097	27209975	ID=MS.gene057607.t1.exon2;Parent=MS.gene057607.t1
chr5.4	CDS	27209975	27210097	27209975	ID=cds.MS.gene057607.t1;Parent=MS.gene057607.t1
chr5.4	exon	27209333	27209464	27209333	ID=MS.gene057607.t1.exon3;Parent=MS.gene057607.t1
chr5.4	CDS	27209333	27209464	27209333	ID=cds.MS.gene057607.t1;Parent=MS.gene057607.t1

Gene Sequence

>MS.gene057607

ATGGGAGGCAATTTATCCAACATGGAGCATGGTCATACATCTGCAAAGTCATTATCATCTCACATTCTTACCTTCCATTCCACTGCTAAATGGAAGGCTTATTTTGATGCCTCCAAAGAAACTAACAAGCTGGTGCGTTATTATTAATTTAATTTAAAGCTATACTAATTATTTTGTATTTTCATTTTGTTTTTTTAACTCTCATGGGATCGTGTATTTTTTTGGCTTAAGATGGTAATTGAATTTACGGCTGCATGGTGTGGACCTTGCAAATACATGGATCCAATCATTCAAGACTTTGCTGCAAAATACATAAAAGTAGATTTCATCAAGATTGACGTGGATGAATTAATGGTATTATTACATTTTTATTTCATTTTACTATTATTATTACTCATGTTATATCTCCTAATATAAGGGCAGCACACCATCTATACATTATATAAAGATGAATGACATATTACTTATAAGCTCTTTTTATTAATTTTACAAATATTATGTAAAACAAAAATAGTGTATTTTACTTATAAGCTCTTACTATGGTCTTTTTCCATACGAACAGGATGACTAAGGCAAAAAAAAAAAAAGTACGAATATTCCTAAAATAACCATTTGAGTATAATTAATGTATCAAACTCCGGCTTTTATATCCCTTAATTCAACTTAACAGTATTAATAAAGAGTATATTTGATAGAAAATAATAAATATATTTCATAACTTGAAATGAGATTTATATTTAAAGATGAACTTTTTTTTTAAAGGGGAGTTATGACTAGACACAAAATTGGTAATTTTTTAGCTACATTTGAAACGAGACTTTTTGAGGAATTGACTTGTGCACATTATTTTTCAATTGTAAACAGGGTGTCTCTCAAGAGTTTCAAGTGCAAGCAATGCCAACATTCATACTGATGAAGAAAGGGAAAGTTGTTGACAAGGTTGTAGGGGCAAAAAAGGAAGAGCTTGAAAAGTTGATTGAGAAGCATCAAAACTAA

Protein sequence

>MS.gene057607.t1

MGGNLSNMEHGHTSAKSLSSHILTFHSTAKWKAYFDASKETNKLMVIEFTAAWCGPCKYMDPIIQDFAAKYIKVDFIKIDVDELMGVSQEFQVQAMPTFILMKKGKVVDKVVGAKKEELEKLIEKHQN