AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene58678

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58678.t1 MTR_2g035760 95.697 488 21 0 1 488 10 497 0.0 900
MS.gene58678.t1 MTR_6g015785 70.757 489 134 4 1 488 14 494 0.0 650
MS.gene58678.t1 MTR_4g094732 46.467 467 219 10 34 484 29 480 2.62e-139 409
MS.gene58678.t1 MTR_2g035790 52.113 426 184 7 73 484 69 488 3.30e-136 402
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58678.t1 AT4G20050 61.790 458 158 5 29 484 62 504 0.0 565
MS.gene58678.t1 AT4G20050 61.522 460 160 5 29 486 37 481 0.0 565
MS.gene58678.t1 AT4G20050 61.522 460 160 5 29 486 37 481 0.0 565
MS.gene58678.t1 AT4G20050 61.522 460 160 5 29 486 37 481 0.0 565
MS.gene58678.t1 AT4G20050 61.522 460 160 5 29 486 37 481 0.0 565
MS.gene58678.t1 AT4G20050 64.962 391 127 3 98 486 1 383 0.0 516
MS.gene58678.t1 AT4G20050 64.962 391 127 3 98 486 1 383 0.0 516
MS.gene58678.t1 AT4G20040 49.173 423 198 4 73 484 67 483 1.99e-136 402

Find 97 sgRNAs with CRISPR-Local

Find 130 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCATTGTAATTGGTGTTTA+TGG 0.186040 2.3:-53474630 MS.gene58678:CDS
AACTCTCAAGGACCTCTTAT+TGG 0.199279 2.3:-53473522 MS.gene58678:CDS
GTTGTTTGTTTCATTGTAAT+TGG 0.263362 2.3:-53474639 MS.gene58678:CDS
TGGTGTACATTGTTACAATA+AGG 0.274737 2.3:-53473207 MS.gene58678:CDS
TACAATAAGGCCACTGGATT+CGG 0.316472 2.3:-53473194 MS.gene58678:CDS
TGCAACGGGGATGAATTTAA+AGG 0.328386 2.3:-53472880 MS.gene58678:CDS
AGGTGTTGAAATTGTTCATT+TGG 0.332781 2.3:-53472958 MS.gene58678:CDS
TTATTGGATTCAAACTTTAG+AGG 0.335089 2.3:-53473506 MS.gene58678:CDS
AGTTTCTTATGTATGTTTCA+TGG 0.352507 2.3:+53473390 None:intergenic
TGCTATCTGAATTGCCTGTT+GGG 0.359312 2.3:+53474277 None:intergenic
TTGATCAAGTTATTGTTGAT+AGG 0.379420 2.3:-53472908 MS.gene58678:CDS
ATGTTCCCAACGCTGGTTAC+CGG 0.382500 2.3:+53474114 None:intergenic
AGAATCATGTCTTGTTAAAG+AGG 0.407306 2.3:+53474530 None:intergenic
CTCTGCTGCTATAGGAATCA+TGG 0.408545 2.3:-53473255 MS.gene58678:CDS
GTTGATAGGAACGTTGCAAC+GGG 0.411337 2.3:-53472894 MS.gene58678:CDS
GCCACTGGATTCGGCGGTAC+CGG 0.416083 2.3:-53473185 MS.gene58678:CDS
CAGTACTCATTAAGTTCAAC+AGG 0.423624 2.3:-53472762 MS.gene58678:CDS
ACATTTCTGATAGCATGATT+AGG 0.424152 2.3:+53472729 None:intergenic
CAGAAAGTCACTCAAGTTAC+CGG 0.424279 2.3:-53474133 MS.gene58678:CDS
TCAATGATGTAGCCATCATT+TGG 0.425679 2.3:+53473614 None:intergenic
CTAGAAATATGAAGTTGAAC+AGG 0.426194 2.3:+53473080 None:intergenic
GCTATAGGAATCATGGTAAC+TGG 0.427357 2.3:-53473248 MS.gene58678:CDS
ACTTGATCAACTTGATGAAA+AGG 0.437301 2.3:+53472921 None:intergenic
TACATAAGAAACTCTTTCCT+TGG 0.440272 2.3:-53473380 MS.gene58678:CDS
ATTTCTAGTAGTTTCTTCCT+TGG 0.441880 2.3:-53473065 MS.gene58678:CDS
ATTGTAGATAACATGTTCTC+TGG 0.444931 2.3:-53472990 MS.gene58678:CDS
TCCATCAAATATCCTTCGCT+TGG 0.449157 2.3:+53474213 None:intergenic
TGCAAGGGAATGGAACTTCA+TGG 0.449669 2.3:-53472836 MS.gene58678:CDS
ATTACACATTTCACAACCAA+TGG 0.452369 2.3:-53473431 MS.gene58678:CDS
TCCAAGCGAAGGATATTTGA+TGG 0.464488 2.3:-53474214 MS.gene58678:CDS
GATGTCACGTGGTATACTCT+TGG 0.468628 2.3:+53474312 None:intergenic
CCTTTCGTGTTTATCGCCTC+CGG 0.470986 2.3:+53473343 None:intergenic
CCTACTAGGAAAATTTCTGA+TGG 0.476121 2.3:-53474594 MS.gene58678:CDS
ATATCCTTCGCTTGGACCGT+TGG 0.476727 2.3:+53474221 None:intergenic
ACCTCATCAAATGAAAATGA+TGG 0.476960 2.3:-53473584 MS.gene58678:CDS
GTGCTATCTGAATTGCCTGT+TGG 0.477189 2.3:+53474276 None:intergenic
AGTTATGTACTCAAAATTGT+AGG 0.480837 2.3:+53473541 None:intergenic
CGCCTCCGGCGGTTATGTGT+TGG 0.483145 2.3:+53473357 None:intergenic
TAAAGGCTACTGTCGCGAAA+AGG 0.486640 2.3:-53472863 MS.gene58678:CDS
TAAAGTTTGAATCCAATAAG+AGG 0.490425 2.3:+53473510 None:intergenic
ACCTCAGCTGGCTGAGAAGA+AGG 0.497308 2.3:+53474471 None:intergenic
TGTATGTTTCATGGCCACTT+TGG 0.501309 2.3:+53473399 None:intergenic
AAAAGGTCGTTGCAAGGGAA+TGG 0.502322 2.3:-53472846 MS.gene58678:CDS
GTATACCACGTGACATCATA+TGG 0.507968 2.3:-53474306 MS.gene58678:CDS
ATTGTATTATATACCTCAGC+TGG 0.511049 2.3:+53474459 None:intergenic
GGTGCACAAATTAATCTTGA+AGG 0.512636 2.3:-53474171 MS.gene58678:CDS
AAGGATTGTGAATTCTTACA+TGG 0.512683 2.3:-53473129 MS.gene58678:CDS
ACCGTTGGCTGCATCTGCTA+TGG 0.521251 2.3:+53474236 None:intergenic
CATTGTTACAATAAGGCCAC+TGG 0.522380 2.3:-53473200 MS.gene58678:CDS
ATGGAAGGCATCAGTAACCT+TGG 0.524813 2.3:-53474195 MS.gene58678:CDS
ACTTTGGACTAAAATTCCAT+TGG 0.525845 2.3:+53473415 None:intergenic
GTCGCGAAAAGGTCGTTGCA+AGG 0.526188 2.3:-53472852 MS.gene58678:CDS
TGTTTGTTTCATGCTTCTTG+AGG 0.528462 2.3:-53474661 None:intergenic
TCATATGGTGCAGACCCAAC+AGG 0.528783 2.3:-53474291 MS.gene58678:CDS
TCAAAATTGTAGGATGATGA+AGG 0.529691 2.3:+53473551 None:intergenic
TCCTTCTTCTCAGCCAGCTG+AGG 0.533497 2.3:-53474472 MS.gene58678:intron
AGGTAGTTTCAAATAAATTC+CGG 0.535034 2.3:+53473166 None:intergenic
AAACACGAAAGGAATTTCTC+AGG 0.538491 2.3:-53473332 MS.gene58678:CDS
AGTTACCGGTAACCAGCGTT+GGG 0.539499 2.3:-53474119 MS.gene58678:CDS
AAGTTACCGGTAACCAGCGT+TGG 0.543200 2.3:-53474120 MS.gene58678:CDS
TGGATTCAAACTTTAGAGGA+GGG 0.543439 2.3:-53473502 MS.gene58678:CDS
TGACAGCATTATCGTTACCT+TGG 0.545887 2.3:+53473294 None:intergenic
GATTAATTTGTGCACCTCCA+AGG 0.547641 2.3:+53474178 None:intergenic
GATTCAAACTTTAGAGGAGG+GGG 0.548301 2.3:-53473500 MS.gene58678:CDS
ATGTTCTCTGGATCAAATCA+AGG 0.548900 2.3:-53472978 MS.gene58678:CDS
GCCATAGCAGATGCAGCCAA+CGG 0.558955 2.3:-53474237 MS.gene58678:CDS
GCTGGCTGAGAAGAAGGAGA+AGG 0.560665 2.3:+53474477 None:intergenic
GGAATTTATTTGAAACTACC+TGG 0.560862 2.3:-53473164 MS.gene58678:CDS
GAAGGTGCAAAAGAAGATGA+TGG 0.564254 2.3:+53474495 None:intergenic
GGATTCAAACTTTAGAGGAG+GGG 0.565517 2.3:-53473501 MS.gene58678:CDS
GAAAGGAATTTCTCAGGCAC+AGG 0.566208 2.3:-53473326 MS.gene58678:CDS
TGTTGATAGGAACGTTGCAA+CGG 0.568090 2.3:-53472895 MS.gene58678:CDS
TTGGATTCAAACTTTAGAGG+AGG 0.571436 2.3:-53473503 MS.gene58678:CDS
GCACAAATTAATCTTGAAGG+TGG 0.580445 2.3:-53474168 MS.gene58678:CDS
TCGCGAAAAGGTCGTTGCAA+GGG 0.583554 2.3:-53472851 MS.gene58678:CDS
TACCATGATGTTCCCAACGC+TGG 0.586939 2.3:+53474107 None:intergenic
AGCGAAGGATATTTGATGGA+AGG 0.588639 2.3:-53474210 MS.gene58678:CDS
CTTGGCCAACACATAACCGC+CGG 0.589091 2.3:-53473362 MS.gene58678:CDS
GAAGGCATCAGTAACCTTGG+AGG 0.592934 2.3:-53474192 MS.gene58678:CDS
ATCCTTGTTTGTGTTAAACC+AGG 0.593807 2.3:+53473146 None:intergenic
CGGCGGTTATGTGTTGGCCA+AGG 0.595647 2.3:+53473363 None:intergenic
AGAGAACATGTTATCTACAA+TGG 0.597546 2.3:+53472992 None:intergenic
TCATGCTATCAGAAATGTGT+CGG 0.600119 2.3:-53472724 MS.gene58678:CDS
TCCGGTACCGCCGAATCCAG+TGG 0.602767 2.3:+53473184 None:intergenic
GCAGCCAACGGTCCAAGCGA+AGG 0.606415 2.3:-53474225 MS.gene58678:CDS
GGCACAGGAATAAACATCCA+AGG 0.625123 2.3:-53473311 MS.gene58678:CDS
AATAAGGCCACTGGATTCGG+CGG 0.629975 2.3:-53473191 MS.gene58678:CDS
TACCTGGTTTAACACAAACA+AGG 0.631189 2.3:-53473148 MS.gene58678:CDS
AACCAGCGTTGGGAACATCA+TGG 0.637299 2.3:-53474109 MS.gene58678:intron
AGCAGAGAAAATTACAACAT+CGG 0.644559 2.3:+53473271 None:intergenic
ATTGTGTTGAAATCTATGAA+AGG 0.648167 2.3:-53473032 MS.gene58678:CDS
GGCCAACACATAACCGCCGG+AGG 0.651132 2.3:-53473359 MS.gene58678:CDS
TTCGTGTTTATCGCCTCCGG+CGG 0.651957 2.3:+53473346 None:intergenic
CTGCACCATATGATGTCACG+TGG 0.660523 2.3:+53474301 None:intergenic
CCGGAGGCGATAAACACGAA+AGG 0.669288 2.3:-53473343 MS.gene58678:CDS
ACACAATATTCGCATCACCA+AGG 0.694010 2.3:+53473048 None:intergenic
TTGATAGGAACGTTGCAACG+GGG 0.722305 2.3:-53472893 MS.gene58678:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAACTAATTATTTGACAAT+TGG - chr2.3:53473375-53473394 MS.gene58678:CDS 15.0%
!! TCATACTGTAAAAAAAAAAA+AGG + chr2.3:53473334-53473353 None:intergenic 15.0%
!!! TTTTTGTTGTTTTAATTTGA+TGG - chr2.3:53473614-53473633 MS.gene58678:CDS 15.0%
!! ATTGTCTCAAAAATTGAATT+TGG + chr2.3:53473427-53473446 None:intergenic 20.0%
!!! ATAATTAGTTTTTCTACAGT+TGG + chr2.3:53473367-53473386 None:intergenic 20.0%
!!! ATGATTTTTTTTTGTTGTGT+AGG - chr2.3:53472949-53472968 MS.gene58678:CDS 20.0%
!!! TAATTTTAGTAGAGAATACT+AGG - chr2.3:53472868-53472887 MS.gene58678:CDS 20.0%
!!! TGATTTTTTTTTGTTGTGTA+GGG - chr2.3:53472950-53472969 MS.gene58678:CDS 20.0%
!!! TTTAGTATTTTTAGTCATGA+TGG - chr2.3:53473456-53473475 MS.gene58678:CDS 20.0%
! AAACTTAGGTACAATGTTAT+AGG + chr2.3:53472901-53472920 None:intergenic 25.0%
! AGGTAGTTTCAAATAAATTC+CGG + chr2.3:53474132-53474151 None:intergenic 25.0%
! AGTTATGTACTCAAAATTGT+AGG + chr2.3:53473757-53473776 None:intergenic 25.0%
! AGTTTCTTATGTATGTTTCA+TGG + chr2.3:53473908-53473927 None:intergenic 25.0%
! ATTGTGTTGAAATCTATGAA+AGG - chr2.3:53474263-53474282 MS.gene58678:CDS 25.0%
! GTTGTTTGTTTCATTGTAAT+TGG - chr2.3:53472656-53472675 MS.gene58678:CDS 25.0%
! TAAAGTTTGAATCCAATAAG+AGG + chr2.3:53473788-53473807 None:intergenic 25.0%
! TTATTGGATTCAAACTTTAG+AGG - chr2.3:53473789-53473808 MS.gene58678:intron 25.0%
! TTGATCAAGTTATTGTTGAT+AGG - chr2.3:53474387-53474406 MS.gene58678:intron 25.0%
!! ATAGTCAATTCTCATTTTCT+TGG + chr2.3:53473504-53473523 None:intergenic 25.0%
!! TGAACATTTTTGATAAGGTT+AGG + chr2.3:53474515-53474534 None:intergenic 25.0%
!! TTCATTGTAATTGGTGTTTA+TGG - chr2.3:53472665-53472684 MS.gene58678:CDS 25.0%
!!! AAAATCATCTGAAGCTTTTA+TGG + chr2.3:53473661-53473680 None:intergenic 25.0%
!!! AGTACTGAACATTTTTGATA+AGG + chr2.3:53474520-53474539 None:intergenic 25.0%
!!! TCTATGAAAGGTGTTTTAAA+TGG - chr2.3:53474275-53474294 MS.gene58678:CDS 25.0%
AAGGATTGTGAATTCTTACA+TGG - chr2.3:53474166-53474185 MS.gene58678:CDS 30.0%
ACATTTCTGATAGCATGATT+AGG + chr2.3:53474569-53474588 None:intergenic 30.0%
ACCTCATCAAATGAAAATGA+TGG - chr2.3:53473711-53473730 MS.gene58678:intron 30.0%
ACTTGATCAACTTGATGAAA+AGG + chr2.3:53474377-53474396 None:intergenic 30.0%
ACTTTGGACTAAAATTCCAT+TGG + chr2.3:53473883-53473902 None:intergenic 30.0%
AGAGAACATGTTATCTACAA+TGG + chr2.3:53474306-53474325 None:intergenic 30.0%
AGCAGAGAAAATTACAACAT+CGG + chr2.3:53474027-53474046 None:intergenic 30.0%
ATTACACATTTCACAACCAA+TGG - chr2.3:53473864-53473883 MS.gene58678:intron 30.0%
ATTGTAGATAACATGTTCTC+TGG - chr2.3:53474305-53474324 MS.gene58678:CDS 30.0%
ATTGTATTATATACCTCAGC+TGG + chr2.3:53472839-53472858 None:intergenic 30.0%
CTAGAAATATGAAGTTGAAC+AGG + chr2.3:53474218-53474237 None:intergenic 30.0%
GTGACAAAAATTTACCTACT+AGG - chr2.3:53472687-53472706 MS.gene58678:CDS 30.0%
TACATAAGAAACTCTTTCCT+TGG - chr2.3:53473915-53473934 MS.gene58678:intron 30.0%
TCAAAATTGTAGGATGATGA+AGG + chr2.3:53473747-53473766 None:intergenic 30.0%
TGGTGTACATTGTTACAATA+AGG - chr2.3:53474088-53474107 MS.gene58678:intron 30.0%
TTGATGGAAAAACAGATACA+TGG - chr2.3:53473630-53473649 MS.gene58678:CDS 30.0%
! AGAATCATGTCTTGTTAAAG+AGG + chr2.3:53472768-53472787 None:intergenic 30.0%
! GGAATTTATTTGAAACTACC+TGG - chr2.3:53474131-53474150 MS.gene58678:CDS 30.0%
! TCAGATGATTTTCCAAATGA+TGG - chr2.3:53473669-53473688 MS.gene58678:intron 30.0%
! TCCATCATTTTCATTTGATG+AGG + chr2.3:53473715-53473734 None:intergenic 30.0%
!! AATGGAATTTTAGTCCAAAG+TGG - chr2.3:53473882-53473901 MS.gene58678:intron 30.0%
!! AGGTGTTGAAATTGTTCATT+TGG - chr2.3:53474337-53474356 MS.gene58678:intron 30.0%
!! ATTTCTAGTAGTTTCTTCCT+TGG - chr2.3:53474230-53474249 MS.gene58678:CDS 30.0%
!! TTTTGAGTACATAACTCTCA+AGG - chr2.3:53473761-53473780 MS.gene58678:intron 30.0%
!!! GGAAGTGTTTTAAGACTTTA+TGG - chr2.3:53473396-53473415 MS.gene58678:CDS 30.0%
!!! TGTTTTAAGACTTTATGGCT+AGG - chr2.3:53473401-53473420 MS.gene58678:CDS 30.0%
AAACACGAAAGGAATTTCTC+AGG - chr2.3:53473963-53473982 MS.gene58678:intron 35.0%
AGCAAATGAAACAATTTCGC+AGG + chr2.3:53473216-53473235 None:intergenic 35.0%
ATGGAAAAACAGATACATGG+AGG - chr2.3:53473633-53473652 MS.gene58678:CDS 35.0%
ATGTTCTCTGGATCAAATCA+AGG - chr2.3:53474317-53474336 MS.gene58678:CDS 35.0%
CAGTACTCATTAAGTTCAAC+AGG - chr2.3:53474533-53474552 MS.gene58678:CDS 35.0%
CCTACTAGGAAAATTTCTGA+TGG - chr2.3:53472701-53472720 MS.gene58678:CDS 35.0%
GCACAAATTAATCTTGAAGG+TGG - chr2.3:53473127-53473146 MS.gene58678:CDS 35.0%
GGTGCACAAATTAATCTTGA+AGG - chr2.3:53473124-53473143 MS.gene58678:CDS 35.0%
GTACCTAAGTTTGTCATTGT+CGG - chr2.3:53472909-53472928 MS.gene58678:CDS 35.0%
TACCTGGTTTAACACAAACA+AGG - chr2.3:53474147-53474166 MS.gene58678:CDS 35.0%
TCAATGATGTAGCCATCATT+TGG + chr2.3:53473684-53473703 None:intergenic 35.0%
TCATGCTATCAGAAATGTGT+CGG - chr2.3:53474571-53474590 MS.gene58678:CDS 35.0%
TTTCCGACAATGACAAACTT+AGG + chr2.3:53472915-53472934 None:intergenic 35.0%
! ATCCTTGTTTGTGTTAAACC+AGG + chr2.3:53474152-53474171 None:intergenic 35.0%
! CCATCAGAAATTTTCCTAGT+AGG + chr2.3:53472704-53472723 None:intergenic 35.0%
! GTAATTTTCTCTGCTGCTAT+AGG - chr2.3:53474032-53474051 MS.gene58678:intron 35.0%
! TGGATTCAAACTTTAGAGGA+GGG - chr2.3:53473793-53473812 MS.gene58678:intron 35.0%
! TTGGATTCAAACTTTAGAGG+AGG - chr2.3:53473792-53473811 MS.gene58678:intron 35.0%
AACTCTCAAGGACCTCTTAT+TGG - chr2.3:53473773-53473792 MS.gene58678:intron 40.0%
ACACAATATTCGCATCACCA+AGG + chr2.3:53474250-53474269 None:intergenic 40.0%
AGCGAAGGATATTTGATGGA+AGG - chr2.3:53473085-53473104 MS.gene58678:CDS 40.0%
CAGAAAGTCACTCAAGTTAC+CGG - chr2.3:53473162-53473181 MS.gene58678:CDS 40.0%
CATTGTTACAATAAGGCCAC+TGG - chr2.3:53474095-53474114 MS.gene58678:intron 40.0%
GCTATAGGAATCATGGTAAC+TGG - chr2.3:53474047-53474066 MS.gene58678:intron 40.0%
GTATACCACGTGACATCATA+TGG - chr2.3:53472989-53473008 MS.gene58678:CDS 40.0%
TACAATAAGGCCACTGGATT+CGG - chr2.3:53474101-53474120 MS.gene58678:intron 40.0%
TCCAAGCGAAGGATATTTGA+TGG - chr2.3:53473081-53473100 MS.gene58678:CDS 40.0%
TCCATCAAATATCCTTCGCT+TGG + chr2.3:53473085-53473104 None:intergenic 40.0%
TGACAGCATTATCGTTACCT+TGG + chr2.3:53474004-53474023 None:intergenic 40.0%
TGCAACGGGGATGAATTTAA+AGG - chr2.3:53474415-53474434 MS.gene58678:intron 40.0%
TGCTATCTGAATTGCCTGTT+GGG + chr2.3:53473021-53473040 None:intergenic 40.0%
TGTATGTTTCATGGCCACTT+TGG + chr2.3:53473899-53473918 None:intergenic 40.0%
! ACTGTAGAAACTGATTCTCG+AGG - chr2.3:53473278-53473297 MS.gene58678:CDS 40.0%
! AGTCATGATGGTGAAGTAGT+TGG - chr2.3:53473468-53473487 MS.gene58678:CDS 40.0%
! GAAGGTGCAAAAGAAGATGA+TGG + chr2.3:53472803-53472822 None:intergenic 40.0%
! GATTCAAACTTTAGAGGAGG+GGG - chr2.3:53473795-53473814 MS.gene58678:intron 40.0%
! GGATTCAAACTTTAGAGGAG+GGG - chr2.3:53473794-53473813 MS.gene58678:intron 40.0%
! GGTCAAGCAAACACATTTTC+TGG - chr2.3:53474068-53474087 MS.gene58678:intron 40.0%
! TGTTGATAGGAACGTTGCAA+CGG - chr2.3:53474400-53474419 MS.gene58678:intron 40.0%
!! GATTAATTTGTGCACCTCCA+AGG + chr2.3:53473120-53473139 None:intergenic 40.0%
AAAAGGTCGTTGCAAGGGAA+TGG - chr2.3:53474449-53474468 MS.gene58678:intron 45.0%
CTCTGCTGCTATAGGAATCA+TGG - chr2.3:53474040-53474059 MS.gene58678:intron 45.0%
GAAAGGAATTTCTCAGGCAC+AGG - chr2.3:53473969-53473988 MS.gene58678:intron 45.0%
GATGTCACGTGGTATACTCT+TGG + chr2.3:53472986-53473005 None:intergenic 45.0%
GGCACAGGAATAAACATCCA+AGG - chr2.3:53473984-53474003 MS.gene58678:intron 45.0%
GTGCTATCTGAATTGCCTGT+TGG + chr2.3:53473022-53473041 None:intergenic 45.0%
TAAAGGCTACTGTCGCGAAA+AGG - chr2.3:53474432-53474451 MS.gene58678:intron 45.0%
TGCAAGGGAATGGAACTTCA+TGG - chr2.3:53474459-53474478 MS.gene58678:intron 45.0%
TTGATAGGAACGTTGCAACG+GGG - chr2.3:53474402-53474421 MS.gene58678:intron 45.0%
! GTTGATAGGAACGTTGCAAC+GGG - chr2.3:53474401-53474420 MS.gene58678:intron 45.0%
!! ATGGAAGGCATCAGTAACCT+TGG - chr2.3:53473100-53473119 MS.gene58678:CDS 45.0%
AAGTTACCGGTAACCAGCGT+TGG - chr2.3:53473175-53473194 MS.gene58678:CDS 50.0%
AATAAGGCCACTGGATTCGG+CGG - chr2.3:53474104-53474123 MS.gene58678:intron 50.0%
AGTTACCGGTAACCAGCGTT+GGG - chr2.3:53473176-53473195 MS.gene58678:CDS 50.0%
ATATCCTTCGCTTGGACCGT+TGG + chr2.3:53473077-53473096 None:intergenic 50.0%
CCTTTCGTGTTTATCGCCTC+CGG + chr2.3:53473955-53473974 None:intergenic 50.0%
CTGCACCATATGATGTCACG+TGG + chr2.3:53472997-53473016 None:intergenic 50.0%
TACCATGATGTTCCCAACGC+TGG + chr2.3:53473191-53473210 None:intergenic 50.0%
TCATATGGTGCAGACCCAAC+AGG - chr2.3:53473004-53473023 MS.gene58678:CDS 50.0%
TCGCGAAAAGGTCGTTGCAA+GGG - chr2.3:53474444-53474463 MS.gene58678:intron 50.0%
! AACCAGCGTTGGGAACATCA+TGG - chr2.3:53473186-53473205 MS.gene58678:CDS 50.0%
! ATGTTCCCAACGCTGGTTAC+CGG + chr2.3:53473184-53473203 None:intergenic 50.0%
!! CGCTGCGAATGTTTTTGTCG+CGG - chr2.3:53474628-53474647 MS.gene58678:CDS 50.0%
!! GAAGGCATCAGTAACCTTGG+AGG - chr2.3:53473103-53473122 MS.gene58678:CDS 50.0%
!! TGCGAATGTTTTTGTCGCGG+TGG - chr2.3:53474631-53474650 MS.gene58678:CDS 50.0%
ACCTCAGCTGGCTGAGAAGA+AGG + chr2.3:53472827-53472846 None:intergenic 55.0%
CCGGAGGCGATAAACACGAA+AGG - chr2.3:53473952-53473971 MS.gene58678:intron 55.0%
CTTGGCCAACACATAACCGC+CGG - chr2.3:53473933-53473952 MS.gene58678:intron 55.0%
GCCATAGCAGATGCAGCCAA+CGG - chr2.3:53473058-53473077 MS.gene58678:CDS 55.0%
GCTGGCTGAGAAGAAGGAGA+AGG + chr2.3:53472821-53472840 None:intergenic 55.0%
GTCGCGAAAAGGTCGTTGCA+AGG - chr2.3:53474443-53474462 MS.gene58678:intron 55.0%
TCCTTCTTCTCAGCCAGCTG+AGG - chr2.3:53472823-53472842 MS.gene58678:CDS 55.0%
TTCGTGTTTATCGCCTCCGG+CGG + chr2.3:53473952-53473971 None:intergenic 55.0%
! ACCGTTGGCTGCATCTGCTA+TGG + chr2.3:53473062-53473081 None:intergenic 55.0%
! CGGCGGTTATGTGTTGGCCA+AGG + chr2.3:53473935-53473954 None:intergenic 60.0%
CGCCTCCGGCGGTTATGTGT+TGG + chr2.3:53473941-53473960 None:intergenic 65.0%
GCAGCCAACGGTCCAAGCGA+AGG - chr2.3:53473070-53473089 MS.gene58678:CDS 65.0%
GCCACTGGATTCGGCGGTAC+CGG - chr2.3:53474110-53474129 MS.gene58678:CDS 65.0%
GGCCAACACATAACCGCCGG+AGG - chr2.3:53473936-53473955 MS.gene58678:intron 65.0%
TCCGGTACCGCCGAATCCAG+TGG + chr2.3:53474114-53474133 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 53472644 53474673 53472644 ID=MS.gene58678
chr2.3 mRNA 53472644 53474673 53472644 ID=MS.gene58678.t1;Parent=MS.gene58678
chr2.3 exon 53474473 53474673 53474473 ID=MS.gene58678.t1.exon1;Parent=MS.gene58678.t1
chr2.3 CDS 53474473 53474673 53474473 ID=cds.MS.gene58678.t1;Parent=MS.gene58678.t1
chr2.3 exon 53474110 53474346 53474110 ID=MS.gene58678.t1.exon2;Parent=MS.gene58678.t1
chr2.3 CDS 53474110 53474346 53474110 ID=cds.MS.gene58678.t1;Parent=MS.gene58678.t1
chr2.3 exon 53472644 53473672 53472644 ID=MS.gene58678.t1.exon3;Parent=MS.gene58678.t1
chr2.3 CDS 53472644 53473672 53472644 ID=cds.MS.gene58678.t1;Parent=MS.gene58678.t1
Gene Sequence

>MS.gene58678

ATGCTTCTTGAGGTTGTTTGTTTCATTGTAATTGGTGTTTATGGTGACAAAAATTTACCTACTAGGAAAATTTCTGATGGAAATTATCATGAAACAATACGAAGATTGCAATCATTTAAAGCCTCTTTAACAAGACATGATTCTATTGTTTCAACACCATCATCTTCTTTTGCACCTTCTCCTTCTTCTCAGCCAGCTGAGGTATATAATACAATCATATACGATAATTTTAGTAGAGAATACTAGGAACAATGCCTATAACATTGTACCTAAGTTTGTCATTGTCGGAAAATATCGAATGTTTGATGATTTTTTTTTGTTGTGTAGGGTATGAACAATCCAAGAGTATACCACGTGACATCATATGGTGCAGACCCAACAGGCAATTCAGATAGCACTGAAGCATTACTTGCAGCCATAGCAGATGCAGCCAACGGTCCAAGCGAAGGATATTTGATGGAAGGCATCAGTAACCTTGGAGGTGCACAAATTAATCTTGAAGGTGGAAATTACATGATCAGAAAGTCACTCAAGTTACCGGTAACCAGCGTTGGGAACATCATGGTACGCCTGCGAAATTGTTTCATTTGCTAATTACATAAACAAAACTATAAATCAAATATCTCTGCGTACAACTGTAGAAACTGATTCTCGAGGATGCACAACTGTAGAAAAATTGCATGCTGTCCTTTTTTTTTTTTACAGTATGAAAAGTATCTACCAACTGTAGAAAAACTAATTATTTGACAATTGGAAGTGTTTTAAGACTTTATGGCTAGGCCAAATTCAATTTTTGAGACAATTAGTCACAATTTAGTATTTTTAGTCATGATGGTGAAGTAGTTGGTCTAACTAGACCAAGAAAATGAGAATTGACTATTTCTATTAACATATTCAAGTTTTTGTTTTTCATTTGTTTATTCAATAAAGCATTCAAAAGAAATCAAAATTTTTGACATGTTAATTTGATTTTTTGTTGTTTTAATTTGATGGAAAAACAGATACATGGAGGAACCATAAAAGCTTCAGATGATTTTCCAAATGATGGCTACATCATTGATTTGTCAACCTCATCAAATGAAAATGATGGAAAAAATTCACCTTCATCATCCTACAATTTTGAGTACATAACTCTCAAGGACCTCTTATTGGATTCAAACTTTAGAGGAGGGGGCATTTCAGTCATAAACTCACTCAGAACAAACATAGACAATTGTTACATTACACATTTCACAACCAATGGAATTTTAGTCCAAAGTGGCCATGAAACATACATAAGAAACTCTTTCCTTGGCCAACACATAACCGCCGGAGGCGATAAACACGAAAGGAATTTCTCAGGCACAGGAATAAACATCCAAGGTAACGATAATGCTGTCACCGATGTTGTAATTTTCTCTGCTGCTATAGGAATCATGGTAACTGGTCAAGCAAACACATTTTCTGGTGTACATTGTTACAATAAGGCCACTGGATTCGGCGGTACCGGAATTTATTTGAAACTACCTGGTTTAACACAAACAAGGATTGTGAATTCTTACATGGATTACACAAGTATTGTTGCTGAAGATCCTGTTCAACTTCATATTTCTAGTAGTTTCTTCCTTGGTGATGCGAATATTGTGTTGAAATCTATGAAAGGTGTTTTAAATGGTGTTACCATTGTAGATAACATGTTCTCTGGATCAAATCAAGGTGTTGAAATTGTTCATTTGGATAAATCAAATAGTCCTTTTCATCAAGTTGATCAAGTTATTGTTGATAGGAACGTTGCAACGGGGATGAATTTAAAGGCTACTGTCGCGAAAAGGTCGTTGCAAGGGAATGGAACTTCATGGAATGTTGATTTTAACAACATTCTTCTTTTTCCTAACCTTATCAAAAATGTTCAGTACTCATTAAGTTCAACAGGTAGCAGTTTTCCTAATCATGCTATCAGAAATGTGTCGGACAATCGCGTTGTGATTGAAACGAATGAAGCAGTCGCTGCGAATGTTTTTGTCGCGGTGGATCAAAGTATGTCAAGTTGA
Protein sequence

>MS.gene58678.t1

MLLEVVCFIVIGVYGDKNLPTRKISDGNYHETIRRLQSFKASLTRHDSIVSTPSSSFAPSPSSQPAEGMNNPRVYHVTSYGADPTGNSDSTEALLAAIADAANGPSEGYLMEGISNLGGAQINLEGGNYMIRKSLKLPVTSVGNIMIHGGTIKASDDFPNDGYIIDLSTSSNENDGKNSPSSSYNFEYITLKDLLLDSNFRGGGISVINSLRTNIDNCYITHFTTNGILVQSGHETYIRNSFLGQHITAGGDKHERNFSGTGINIQGNDNAVTDVVIFSAAIGIMVTGQANTFSGVHCYNKATGFGGTGIYLKLPGLTQTRIVNSYMDYTSIVAEDPVQLHISSSFFLGDANIVLKSMKGVLNGVTIVDNMFSGSNQGVEIVHLDKSNSPFHQVDQVIVDRNVATGMNLKATVAKRSLQGNGTSWNVDFNNILLFPNLIKNVQYSLSSTGSSFPNHAIRNVSDNRVVIETNEAVAANVFVAVDQSMSS