AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene58707

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58707.t1 MTR_2g438110 99.123 342 3 0 1 342 1 342 0.0 701
MS.gene58707.t1 MTR_2g039960 65.696 309 106 0 34 342 41 349 3.02e-151 432
MS.gene58707.t1 MTR_2g099620 64.401 309 110 0 34 342 41 349 2.38e-149 427
MS.gene58707.t1 MTR_2g099620 64.401 309 110 0 34 342 25 333 3.26e-149 427
MS.gene58707.t1 MTR_4g125970 65.049 309 108 0 34 342 41 349 5.05e-148 424
MS.gene58707.t1 MTR_4g125970 65.049 309 108 0 34 342 58 366 9.91e-148 424
MS.gene58707.t1 MTR_8g018400 63.754 309 112 0 34 342 41 349 1.64e-147 423
MS.gene58707.t1 MTR_1g046570 41.768 328 156 3 52 344 9 336 5.23e-82 255
MS.gene58707.t1 MTR_8g018370 42.908 282 136 4 63 342 2 260 1.26e-71 226
MS.gene58707.t1 MTR_7g076820 36.503 326 203 3 26 349 140 463 6.05e-70 228
MS.gene58707.t1 MTR_7g076800 36.196 326 204 3 26 349 131 454 1.03e-69 227
MS.gene58707.t1 MTR_7g076830 36.196 326 204 3 26 349 133 456 1.05e-69 227
MS.gene58707.t1 MTR_0915s0010 36.396 283 178 2 47 329 2 282 1.94e-63 204
MS.gene58707.t1 MTR_2g100230 30.899 356 232 4 1 349 69 417 3.09e-56 191
MS.gene58707.t1 MTR_1g012420 35.000 320 200 7 28 342 42 358 5.84e-52 178
MS.gene58707.t1 MTR_4g105100 33.435 329 213 2 17 339 185 513 7.91e-52 182
MS.gene58707.t1 MTR_4g105100 33.129 326 212 2 17 336 185 510 5.21e-51 178
MS.gene58707.t1 MTR_3g107420 34.769 325 203 7 25 342 36 358 2.04e-50 174
MS.gene58707.t1 MTR_4g010565 34.650 329 202 8 25 342 109 435 6.20e-50 174
MS.gene58707.t1 MTR_7g109720 32.390 318 205 4 30 339 228 543 2.60e-49 177
MS.gene58707.t1 MTR_3g467550 31.003 329 209 6 32 344 115 441 5.67e-48 168
MS.gene58707.t1 MTR_2g032630 31.410 312 207 5 34 339 496 806 1.94e-47 172
MS.gene58707.t1 MTR_3g467550 31.003 329 209 6 32 344 115 441 2.29e-47 169
MS.gene58707.t1 MTR_4g049910 32.808 317 200 5 33 342 54 364 2.29e-46 164
MS.gene58707.t1 MTR_1g061970 33.642 324 198 9 34 342 80 401 4.28e-46 164
MS.gene58707.t1 MTR_3g083690 30.986 355 214 7 17 344 126 476 1.30e-45 165
MS.gene58707.t1 MTR_3g467550 29.248 359 233 8 3 344 56 410 3.49e-45 162
MS.gene58707.t1 MTR_5g090120 28.571 336 227 6 13 338 58 390 8.89e-45 162
MS.gene58707.t1 MTR_3g449250 30.218 321 211 5 37 349 93 408 1.24e-44 161
MS.gene58707.t1 MTR_3g449250 30.968 310 203 4 37 338 93 399 1.41e-44 161
MS.gene58707.t1 MTR_4g114120 32.143 336 199 6 35 344 141 473 2.69e-44 162
MS.gene58707.t1 MTR_6g005550 31.111 315 204 7 34 339 165 475 8.55e-43 158
MS.gene58707.t1 MTR_4g134170 26.571 350 231 4 20 344 273 621 1.08e-41 155
MS.gene58707.t1 MTR_1g054630 29.830 352 221 8 17 344 135 484 1.72e-41 153
MS.gene58707.t1 MTR_4g079320 30.421 309 209 4 35 338 20 327 1.33e-40 152
MS.gene58707.t1 MTR_6g005320 26.286 350 230 5 20 344 235 581 3.29e-40 150
MS.gene58707.t1 MTR_6g082080 27.937 315 217 4 30 338 6 316 8.95e-40 146
MS.gene58707.t1 MTR_7g056830 27.937 315 217 4 30 338 6 316 9.62e-40 146
MS.gene58707.t1 MTR_5g027840 29.003 331 221 4 35 353 105 433 2.83e-38 143
MS.gene58707.t1 MTR_1g095810 30.124 322 207 6 31 337 142 460 6.18e-38 143
MS.gene58707.t1 MTR_1g026830 29.193 322 210 6 31 337 144 462 3.36e-37 141
MS.gene58707.t1 MTR_2g007000 29.154 319 218 2 30 340 500 818 7.27e-35 136
MS.gene58707.t1 MTR_6g033070 27.554 323 206 6 34 338 140 452 1.31e-34 133
MS.gene58707.t1 MTR_3g049990 29.936 314 208 6 34 338 152 462 2.05e-34 134
MS.gene58707.t1 MTR_2g066050 27.619 315 215 4 33 338 52 362 8.61e-32 125
MS.gene58707.t1 MTR_7g100820 29.430 316 211 5 32 339 128 439 1.40e-31 125
MS.gene58707.t1 MTR_2g063080 25.876 371 212 7 31 340 106 474 1.11e-30 122
MS.gene58707.t1 MTR_3g107420 35.849 212 129 5 25 230 36 246 4.28e-30 117
MS.gene58707.t1 MTR_4g112340 26.923 312 211 7 46 344 109 416 5.41e-30 120
MS.gene58707.t1 MTR_2g041140 27.778 324 226 6 23 338 129 452 1.28e-29 120
MS.gene58707.t1 MTR_6g039840 25.904 332 219 7 31 338 107 435 2.88e-29 119
MS.gene58707.t1 MTR_2g055850 26.235 324 221 7 28 338 85 403 3.00e-29 118
MS.gene58707.t1 MTR_3g071620 27.607 326 208 7 40 338 22 346 1.44e-28 117
MS.gene58707.t1 MTR_8g027770 25.758 330 210 6 35 337 202 523 3.40e-27 113
MS.gene58707.t1 MTR_8g027775 26.409 337 219 6 35 347 103 434 4.05e-27 112
MS.gene58707.t1 MTR_8g027780 26.176 340 222 6 35 350 107 441 6.63e-27 111
MS.gene58707.t1 MTR_5g092560 29.245 318 198 9 57 359 99 404 6.64e-27 112
MS.gene58707.t1 MTR_6g056080 28.571 301 186 7 59 339 128 419 6.64e-27 112
MS.gene58707.t1 MTR_8g097000 25.698 358 225 11 10 337 264 610 1.55e-26 111
MS.gene58707.t1 MTR_1g083130 26.254 339 224 8 14 331 6 339 3.16e-26 110
MS.gene58707.t1 MTR_8g027780 26.331 338 212 8 35 344 107 435 1.52e-25 108
MS.gene58707.t1 MTR_4g068990 29.167 312 198 9 61 359 102 403 1.66e-25 108
MS.gene58707.t1 MTR_7g057250 24.474 380 211 9 34 338 163 541 2.40e-25 107
MS.gene58707.t1 MTR_1g102410 26.586 331 208 6 40 339 50 376 1.20e-24 105
MS.gene58707.t1 MTR_4g077370 27.919 197 139 1 34 227 250 446 2.21e-21 95.1
MS.gene58707.t1 MTR_4g077370 27.919 197 116 4 34 223 2 179 6.16e-17 82.0
MS.gene58707.t1 MTR_8g018350 47.619 105 46 4 245 342 15 117 4.03e-21 89.7
MS.gene58707.t1 MTR_8g032230 32.857 140 93 1 30 168 6 145 1.73e-19 85.1
MS.gene58707.t1 MTR_3g118120 25.000 360 232 9 17 344 121 474 3.22e-19 89.4
MS.gene58707.t1 MTR_6g039840 26.295 251 167 6 103 338 12 259 1.91e-18 86.7
MS.gene58707.t1 MTR_4g112320 26.761 213 151 4 136 344 23 234 1.30e-17 84.0
MS.gene58707.t1 MTR_4g131750 25.434 346 205 12 40 344 88 421 2.36e-17 83.6
MS.gene58707.t1 MTR_2g043360 25.769 260 165 7 3 234 9 268 3.09e-17 83.6
MS.gene58707.t1 MTR_8g018330 41.509 106 56 3 245 344 15 120 3.52e-17 79.0
MS.gene58707.t1 MTR_4g059460 26.471 238 148 6 24 234 34 271 5.82e-17 82.4
MS.gene58707.t1 MTR_4g459350 27.513 189 128 4 24 203 31 219 8.43e-17 80.1
MS.gene58707.t1 MTR_5g092560 28.723 188 124 4 176 359 12 193 1.61e-13 72.0
MS.gene58707.t1 MTR_4g068990 28.191 188 124 5 176 359 12 192 3.09e-13 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58707.t1 AT3G19760 83.430 344 55 1 1 342 1 344 0.0 596
MS.gene58707.t1 AT1G51380 73.313 326 87 0 19 344 8 333 0.0 514
MS.gene58707.t1 AT1G51380 74.906 267 67 0 19 285 8 274 6.93e-152 429
MS.gene58707.t1 AT3G13920 65.696 309 106 0 34 342 40 348 4.47e-151 432
MS.gene58707.t1 AT3G13920 65.696 309 106 0 34 342 30 338 5.08e-151 431
MS.gene58707.t1 AT3G13920 65.696 309 106 0 34 342 40 348 4.50e-150 429
MS.gene58707.t1 AT3G13920 65.696 309 106 0 34 342 44 352 4.72e-150 429
MS.gene58707.t1 AT1G54270 64.725 309 109 0 34 342 40 348 9.21e-149 426
MS.gene58707.t1 AT1G72730 64.725 309 109 0 34 342 42 350 2.07e-147 422
MS.gene58707.t1 AT3G13920 64.401 309 105 1 34 342 40 343 8.13e-145 416
MS.gene58707.t1 AT1G54270 63.430 309 108 1 34 342 40 343 1.13e-142 410
MS.gene58707.t1 AT1G51380 75.221 226 56 0 19 244 8 233 7.70e-125 361
MS.gene58707.t1 AT1G51380 75.221 226 56 0 19 244 8 233 1.41e-124 360
MS.gene58707.t1 AT4G00660 37.117 326 201 3 26 349 122 445 9.54e-72 232
MS.gene58707.t1 AT4G00660 37.117 326 201 3 26 349 122 445 9.54e-72 232
MS.gene58707.t1 AT3G61240 35.347 331 211 3 20 349 110 438 6.08e-70 227
MS.gene58707.t1 AT3G61240 35.347 331 211 3 20 349 110 438 6.08e-70 227
MS.gene58707.t1 AT2G45810 35.583 326 206 3 26 349 145 468 9.53e-68 222
MS.gene58707.t1 AT1G55150 32.622 328 211 3 30 349 96 421 1.39e-57 195
MS.gene58707.t1 AT1G55150 32.622 328 211 3 30 349 121 446 2.88e-57 194
MS.gene58707.t1 AT3G53110 34.796 319 196 7 35 342 93 410 1.72e-52 181
MS.gene58707.t1 AT5G63120 33.639 327 209 4 20 339 152 477 1.54e-51 181
MS.gene58707.t1 AT5G63120 33.333 324 208 4 20 336 152 474 7.01e-51 177
MS.gene58707.t1 AT5G14610 33.951 324 205 6 34 349 170 492 7.38e-51 177
MS.gene58707.t1 AT3G01540 35.256 312 195 5 34 339 159 469 7.63e-51 179
MS.gene58707.t1 AT3G01540 35.256 312 195 5 34 339 159 469 7.63e-51 179
MS.gene58707.t1 AT3G01540 35.256 312 195 5 34 339 159 469 7.63e-51 179
MS.gene58707.t1 AT3G01540 35.256 312 195 5 34 339 159 469 7.92e-51 179
MS.gene58707.t1 AT5G63120 33.333 324 208 4 20 336 152 474 1.07e-50 176
MS.gene58707.t1 AT5G63120 33.333 324 208 4 20 336 152 474 1.07e-50 176
MS.gene58707.t1 AT5G63120 33.333 324 208 4 20 336 152 474 1.07e-50 176
MS.gene58707.t1 AT5G11170 34.062 320 203 7 28 342 42 358 2.09e-50 174
MS.gene58707.t1 AT5G11200 34.062 320 203 7 28 342 42 358 2.09e-50 174
MS.gene58707.t1 AT3G06480 33.333 330 209 5 34 355 436 762 3.55e-50 179
MS.gene58707.t1 AT5G14610 33.974 312 199 5 34 339 162 472 6.35e-49 174
MS.gene58707.t1 AT5G14610 33.974 312 199 5 34 339 170 480 6.61e-49 174
MS.gene58707.t1 AT5G14610 33.974 312 199 5 34 339 162 472 6.73e-49 174
MS.gene58707.t1 AT3G06480 34.395 314 195 5 34 339 436 746 9.40e-49 176
MS.gene58707.t1 AT2G47330 31.395 344 212 6 15 339 202 540 1.76e-48 174
MS.gene58707.t1 AT3G22330 30.060 336 198 9 34 349 105 423 3.66e-46 166
MS.gene58707.t1 AT3G22310 29.814 322 211 6 37 349 120 435 2.99e-45 164
MS.gene58707.t1 AT3G22310 29.814 322 211 6 37 349 120 435 3.57e-45 164
MS.gene58707.t1 AT5G14610 33.221 298 192 5 48 339 45 341 1.05e-44 160
MS.gene58707.t1 AT5G14610 33.221 298 192 5 48 339 45 341 1.05e-44 160
MS.gene58707.t1 AT5G26742 33.443 305 182 8 59 349 128 425 8.08e-44 161
MS.gene58707.t1 AT5G26742 33.443 305 182 8 59 349 128 425 8.13e-44 161
MS.gene58707.t1 AT5G14610 33.333 300 193 5 46 339 242 540 1.05e-43 160
MS.gene58707.t1 AT2G42520 31.180 356 211 9 17 344 141 490 1.34e-43 159
MS.gene58707.t1 AT2G42520 31.180 356 211 9 17 344 141 490 1.34e-43 159
MS.gene58707.t1 AT2G42520 31.180 356 211 9 17 344 141 490 1.34e-43 159
MS.gene58707.t1 AT2G33730 28.529 340 217 4 30 344 310 648 2.42e-43 160
MS.gene58707.t1 AT5G26742 33.993 303 178 9 62 349 37 332 4.64e-43 158
MS.gene58707.t1 AT3G58510 30.659 349 220 7 17 344 133 480 5.51e-43 157
MS.gene58707.t1 AT3G58510 30.659 349 220 7 17 344 133 480 5.51e-43 157
MS.gene58707.t1 AT3G58510 30.659 349 220 7 17 344 133 480 5.51e-43 157
MS.gene58707.t1 AT5G11200 30.194 361 203 8 28 342 42 399 2.28e-42 154
MS.gene58707.t1 AT1G77030 31.190 311 204 5 35 338 30 337 8.49e-42 156
MS.gene58707.t1 AT3G58570 30.337 356 214 8 17 344 128 477 1.10e-41 154
MS.gene58707.t1 AT5G11170 33.696 276 176 6 72 342 2 275 8.00e-41 147
MS.gene58707.t1 AT5G60990 28.882 322 211 5 32 338 8 326 1.20e-38 143
MS.gene58707.t1 AT4G33370 30.841 321 204 6 32 337 96 413 3.97e-38 143
MS.gene58707.t1 AT5G51280 31.153 321 203 6 32 337 145 462 1.53e-37 142
MS.gene58707.t1 AT3G09720 31.132 318 203 6 32 339 136 447 4.71e-37 140
MS.gene58707.t1 AT3G09720 31.132 318 203 6 32 339 136 447 4.71e-37 140
MS.gene58707.t1 AT3G02065 27.273 319 213 5 34 338 111 424 5.87e-37 139
MS.gene58707.t1 AT3G02065 27.273 319 213 5 34 338 111 424 5.87e-37 139
MS.gene58707.t1 AT1G20920 29.688 320 215 5 30 340 188 506 9.36e-37 141
MS.gene58707.t1 AT1G31970 28.349 321 215 4 31 338 114 432 6.24e-36 137
MS.gene58707.t1 AT1G20920 29.688 320 215 5 30 340 526 844 2.71e-35 137
MS.gene58707.t1 AT1G20920 29.688 320 215 5 30 340 526 844 2.71e-35 137
MS.gene58707.t1 AT1G20920 29.688 320 215 5 30 340 526 844 2.71e-35 137
MS.gene58707.t1 AT1G20920 29.688 320 215 5 30 340 526 844 2.71e-35 137
MS.gene58707.t1 AT1G20920 29.688 320 215 5 30 340 526 844 2.71e-35 137
MS.gene58707.t1 AT1G20920 29.688 320 215 5 30 340 526 844 2.71e-35 137
MS.gene58707.t1 AT1G12770 26.158 367 209 6 34 340 111 475 1.69e-34 133
MS.gene58707.t1 AT1G12770 26.158 367 209 6 34 340 111 475 1.69e-34 133
MS.gene58707.t1 AT4G16630 30.341 323 218 4 23 338 156 478 8.32e-34 132
MS.gene58707.t1 AT1G16280 29.968 317 203 8 34 338 59 368 1.24e-33 130
MS.gene58707.t1 AT3G18600 27.385 325 228 5 27 344 83 406 1.55e-33 130
MS.gene58707.t1 AT1G71370 28.313 332 220 8 30 344 11 341 3.96e-33 129
MS.gene58707.t1 AT1G28180 31.010 287 183 7 63 338 2 284 1.24e-32 125
MS.gene58707.t1 AT3G02065 27.148 291 193 5 62 338 2 287 2.29e-32 124
MS.gene58707.t1 AT5G05450 27.410 332 216 7 37 344 20 350 3.42e-32 127
MS.gene58707.t1 AT5G65900 27.554 323 217 5 34 344 155 472 2.49e-31 125
MS.gene58707.t1 AT5G11200 33.333 243 146 7 28 266 42 272 4.47e-31 123
MS.gene58707.t1 AT5G63630 26.506 332 205 8 35 337 56 377 2.21e-30 121
MS.gene58707.t1 AT5G54910 28.235 340 224 10 31 359 69 399 3.96e-30 122
MS.gene58707.t1 AT5G63630 27.019 322 196 8 45 337 260 571 1.48e-29 120
MS.gene58707.t1 AT5G62190 28.617 311 187 7 52 339 115 413 7.15e-29 118
MS.gene58707.t1 AT5G08620 27.300 337 206 9 35 342 82 408 3.31e-28 115
MS.gene58707.t1 AT1G71280 27.564 312 186 8 40 338 24 308 4.91e-28 112
MS.gene58707.t1 AT1G71280 27.726 321 192 9 40 347 24 317 5.52e-28 114
MS.gene58707.t1 AT5G08610 27.434 339 207 9 35 344 384 712 7.75e-28 115
MS.gene58707.t1 AT4G34910 27.900 319 208 8 31 330 44 359 1.65e-27 114
MS.gene58707.t1 AT2G07750 27.536 345 208 10 24 337 366 699 6.06e-27 112
MS.gene58707.t1 AT1G63250 26.462 359 222 10 10 337 305 652 7.09e-27 112
MS.gene58707.t1 AT3G09620 27.187 320 194 6 30 340 394 683 1.09e-26 112
MS.gene58707.t1 AT3G09620 27.187 320 194 6 30 340 393 682 1.12e-26 112
MS.gene58707.t1 AT4G09730 23.677 359 239 8 8 338 87 438 1.20e-25 108
MS.gene58707.t1 AT3G06980 25.137 366 234 10 12 337 344 709 8.69e-21 94.4
MS.gene58707.t1 AT2G40700 23.529 391 218 11 28 337 25 415 1.54e-19 90.5
MS.gene58707.t1 AT5G19210 24.189 339 215 10 40 344 83 413 4.19e-18 85.9
MS.gene58707.t1 AT3G16840 26.486 185 112 3 44 204 201 385 1.81e-14 75.1
MS.gene58707.t1 AT3G16840 26.486 185 112 3 44 204 202 386 1.85e-14 75.1
MS.gene58707.t1 AT1G28180 31.532 111 68 2 68 170 8 118 7.06e-11 59.7

Find 94 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGGGAAAGAGATGCCATTA+TGG 0.307907 2.3:+53041937 MS.gene58707:CDS
TATTGATAACAACCGATGTT+TGG 0.322422 2.3:+53041987 MS.gene58707:CDS
GACTTCGATGATTGCCTTGA+CGG 0.331628 2.3:+53039289 MS.gene58707:CDS
GAGAAAGTTATATTGGCAAT+TGG 0.345232 2.3:+53040391 MS.gene58707:CDS
ATATCTCCCACCGGATCTTC+AGG 0.347012 2.3:+53040744 MS.gene58707:CDS
AGAAAGTTATATTGGCAATT+GGG 0.352858 2.3:+53040392 MS.gene58707:CDS
GGCCATCAAGTTGCTAGTTC+TGG 0.359331 2.3:+53040561 MS.gene58707:CDS
ACAGACTGAGAAAGTTATAT+TGG 0.367040 2.3:+53040384 MS.gene58707:CDS
TACGCTACCTCATGAAATTC+TGG 0.380878 2.3:+53041430 MS.gene58707:CDS
GGCGACAGCTTCTGTGGTTC+CGG 0.382833 2.3:+53039043 MS.gene58707:CDS
ATTGATAACAACCGATGTTT+GGG 0.383191 2.3:+53041988 MS.gene58707:CDS
ACATCCCTTCCTTGAATAAT+CGG 0.387301 2.3:-53039236 None:intergenic
ATGGCATCTCTTTCCCTTTG+AGG 0.391683 2.3:-53041932 None:intergenic
CACGGAGTTCATGTCGTGTC+TGG 0.393969 2.3:+53040487 MS.gene58707:CDS
AGCACGGAATTCACCCATAA+TGG 0.405284 2.3:-53041951 None:intergenic
TACCAGAACTAGCAACTTGA+TGG 0.419216 2.3:-53040563 None:intergenic
TCCGATTGCTTTCACGCCTT+CGG 0.420202 2.3:-53039117 None:intergenic
GATTGCCTTGACGGTTTGTC+AGG 0.429119 2.3:+53039298 MS.gene58707:CDS
AGGGAAAGAGATGCCATTAT+GGG 0.441532 2.3:+53041938 MS.gene58707:CDS
CATCGGTTGTTATCAATACT+CGG 0.443307 2.3:-53041982 None:intergenic
GAGGTAGCGTAGCAGAAATC+AGG 0.468804 2.3:-53041418 None:intergenic
GTTATTGCTCAGGCGCAATC+TGG 0.470550 2.3:+53039257 MS.gene58707:CDS
CAAGCCCATGCTTGCATTGG+AGG 0.471982 2.3:+53040430 MS.gene58707:CDS
TCGTGGCCTTGATGTACAAC+AGG 0.473001 2.3:+53042012 MS.gene58707:CDS
AGAGGAGAACATTGCGCACC+AGG 0.473245 2.3:+53040539 MS.gene58707:CDS
GAATCGCCGACGAACGGTTA+CGG 0.475337 2.3:+53039067 MS.gene58707:CDS
AATCGGAAGCTTCGAAGAGA+TGG 0.477193 2.3:+53039133 MS.gene58707:CDS
TGTGAGGCCAGTTCTCTTGT+CGG 0.478916 2.3:-53040364 None:intergenic
CTTCGTGGAATCTACCAATA+CGG 0.479176 2.3:+53039173 MS.gene58707:CDS
TGTGTCTCCGACAAGAGAAC+TGG 0.480029 2.3:+53040357 MS.gene58707:CDS
AAGGTTTCTCAAATCCGTAT+TGG 0.482981 2.3:-53039187 None:intergenic
AGGAAGGGATGTTATTGCTC+AGG 0.483228 2.3:+53039247 MS.gene58707:CDS
GCTCAGGCGCAATCTGGTAC+TGG 0.490296 2.3:+53039263 MS.gene58707:CDS
TCAATTCATCACGCTTTACA+AGG 0.494715 2.3:-53041490 None:intergenic
ACCGTTCGTCGGCGATTCGC+CGG 0.496560 2.3:-53039062 None:intergenic
ACCGAAGGCGTGAAAGCAAT+CGG 0.496626 2.3:+53039116 MS.gene58707:CDS
TCCCCTTGGAACAGGTGGAC+TGG 0.499263 2.3:+53041852 MS.gene58707:intron
ATCGGAAGCTTCGAAGAGAT+GGG 0.501930 2.3:+53039134 MS.gene58707:CDS
ATAAAAGACGATTTGCTTCG+TGG 0.502129 2.3:+53039158 MS.gene58707:CDS
TTTCCCTCCAATGCAAGCAT+GGG 0.504031 2.3:-53040434 None:intergenic
CATGTCGTGTCTGGAACTCC+TGG 0.508662 2.3:+53040496 MS.gene58707:CDS
ATACAAGCCCATGCTTGCAT+TGG 0.509573 2.3:+53040427 MS.gene58707:CDS
GAAAGTTATATTGGCAATTG+GGG 0.511942 2.3:+53040393 MS.gene58707:CDS
GTAAAGAGAACCTGAAGATC+CGG 0.512664 2.3:-53040754 None:intergenic
GCTGTGGCGCCGATTATTCA+AGG 0.516832 2.3:+53039227 MS.gene58707:CDS
TTGAGGCATGTCACCGTGCA+TGG 0.517494 2.3:-53041915 None:intergenic
ACAACCGATGTTTGGGCTCG+TGG 0.522556 2.3:+53041995 MS.gene58707:CDS
AACTAGCAACTTGATGGCCC+TGG 0.522591 2.3:-53040557 None:intergenic
CGCTTTACAAGGATCCTCAC+TGG 0.522851 2.3:-53041479 None:intergenic
ATCATGTCACAGACTCGGCC+AGG 0.526550 2.3:-53040514 None:intergenic
TTGCGGTTGAAAGGGAGGAA+TGG 0.531108 2.3:+53041621 MS.gene58707:CDS
TGCACGGTGACATGCCTCAA+AGG 0.534214 2.3:+53041918 MS.gene58707:CDS
CTGATGAAATGTTGAGCAGA+GGG 0.540674 2.3:+53040692 MS.gene58707:CDS
GGTTACGGCGAACGAGGACA+TGG 0.543356 2.3:+53039082 MS.gene58707:CDS
TGCGATTCAACAGCGTGCTG+TGG 0.544984 2.3:+53039211 MS.gene58707:CDS
GCACGGTGACATGCCTCAAA+GGG 0.551418 2.3:+53041919 MS.gene58707:CDS
AACTTGCCTGTTGTACATCA+AGG 0.552831 2.3:-53042018 None:intergenic
ATCGGTTGTTATCAATACTC+GGG 0.555470 2.3:-53041981 None:intergenic
TTCACTGTTTCATCCATGCA+CGG 0.556873 2.3:+53041902 MS.gene58707:CDS
TGGCGCCGATTATTCAAGGA+AGG 0.572845 2.3:+53039231 MS.gene58707:CDS
GCGTGATGAATTGACATTGG+AGG 0.575652 2.3:+53041499 MS.gene58707:CDS
GGCGATGGCGACAGCTTCTG+TGG 0.581450 2.3:+53039037 None:intergenic
CGTTCGCCGTAACCGTTCGT+CGG 0.582245 2.3:-53039073 None:intergenic
AAGCCCATGCTTGCATTGGA+GGG 0.587223 2.3:+53040431 MS.gene58707:CDS
GAGGAGAACATTGCGCACCA+GGG 0.595037 2.3:+53040540 MS.gene58707:CDS
TCCGGCGAATCGCCGACGAA+CGG 0.596292 2.3:+53039061 MS.gene58707:CDS
AATAATGATAAAATGCACAC+TGG 0.597948 2.3:-53042047 None:intergenic
GAGTCTGTGACATGATAAAG+AGG 0.598721 2.3:+53040521 MS.gene58707:CDS
ATTATGGGTGAATTCCGTGC+TGG 0.607873 2.3:+53041953 MS.gene58707:CDS
GACATTAGAAAACTTGAGCA+CGG 0.609708 2.3:+53040469 MS.gene58707:CDS
GCACGCTGTTGAATCGCAGA+AGG 0.610346 2.3:-53039206 None:intergenic
GAATATAACAGCTTGAGTAA+TGG 0.612678 2.3:-53041681 None:intergenic
AAAGCGTGATGAATTGACAT+TGG 0.623480 2.3:+53041496 MS.gene58707:CDS
AAGAGAACCTGAAGATCCGG+TGG 0.623806 2.3:-53040751 None:intergenic
TCTTTATCATGTCACAGACT+CGG 0.625391 2.3:-53040519 None:intergenic
AGAAGAAACTCACTCTCTGA+CGG 0.631263 2.3:-53039333 None:intergenic
TTATATTCTGCAACACCAAG+CGG 0.634758 2.3:+53041696 MS.gene58707:CDS
TGCATTGGAGGGAAAAGTGT+GGG 0.639623 2.3:+53040442 MS.gene58707:CDS
ATTCTGCAACACCAAGCGGA+AGG 0.643180 2.3:+53041700 MS.gene58707:CDS
AACAACCTGACAAACCGTCA+AGG 0.644356 2.3:-53039303 None:intergenic
ACGAACGGTTACGGCGAACG+AGG 0.645748 2.3:+53039076 MS.gene58707:CDS
GGCGCCGATTATTCAAGGAA+GGG 0.657629 2.3:+53039232 MS.gene58707:CDS
GTCATCTCCAGAATTTCATG+AGG 0.665661 2.3:-53041437 None:intergenic
TCGGAAGCTTCGAAGAGATG+GGG 0.670150 2.3:+53039135 MS.gene58707:CDS
AGAAACTCACTCTCTGACGG+AGG 0.672890 2.3:-53039330 None:intergenic
TGTGTACAGATATCTCCCAC+CGG 0.677582 2.3:+53040735 MS.gene58707:CDS
AGAGAACCTGAAGATCCGGT+GGG 0.679640 2.3:-53040750 None:intergenic
AAGGCCACGAGCCCAAACAT+CGG 0.689261 2.3:-53041999 None:intergenic
TATAACTTTCTCAGTCTGTG+AGG 0.699081 2.3:-53040380 None:intergenic
ATACTCGGGTTGTTCCAGCA+CGG 0.705926 2.3:-53041967 None:intergenic
TCTGATGAAATGTTGAGCAG+AGG 0.713686 2.3:+53040691 MS.gene58707:CDS
TTGCATTGGAGGGAAAAGTG+TGG 0.730153 2.3:+53040441 MS.gene58707:CDS
AGTTTATGACAGATCCAGTG+AGG 0.735827 2.3:+53041465 MS.gene58707:CDS
ATGGATTTCGAAACTACCGA+AGG 0.736816 2.3:+53039101 MS.gene58707:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTAATATGTTTTCTTTATT+TGG + chr2.3:53041196-53041215 MS.gene58707:intron 10.0%
!!! TAGTTCTTTTAATTTATAAA+TGG + chr2.3:53040180-53040199 MS.gene58707:intron 10.0%
!!! ACAAAAATAGTATTGAATAT+TGG + chr2.3:53040925-53040944 MS.gene58707:intron 15.0%
!!! ATATTTTTCTTTTCAAATAC+AGG + chr2.3:53040663-53040682 MS.gene58707:intron 15.0%
!!! TTAGTTAATTTGTTTGAATT+TGG + chr2.3:53039383-53039402 MS.gene58707:intron 15.0%
!!! TTGTTTTATGTTAATTTGTT+TGG + chr2.3:53039493-53039512 MS.gene58707:intron 15.0%
!! ACTAAATCCAATTATCAAAT+GGG - chr2.3:53041023-53041042 None:intergenic 20.0%
!! ATAAAATGAGGTAAAATTTC+AGG - chr2.3:53041248-53041267 None:intergenic 20.0%
!! CTATTAAATAAGGATATAAG+AGG + chr2.3:53040986-53041005 MS.gene58707:intron 20.0%
!! TATAAATAGAGAGTAATTTG+AGG + chr2.3:53039538-53039557 MS.gene58707:intron 20.0%
!! TATATGCTTAAAACAAACTA+AGG + chr2.3:53040838-53040857 MS.gene58707:intron 20.0%
!!! AATTTGTTTGGATAAACAAT+TGG + chr2.3:53039505-53039524 MS.gene58707:intron 20.0%
!!! AATTTTGTTATTTGTTGACA+GGG + chr2.3:53040321-53040340 MS.gene58707:intron 20.0%
!!! ACAAAATGTGCTATTAAATA+AGG + chr2.3:53040976-53040995 MS.gene58707:intron 20.0%
!!! TATTTTTTCGTAATATAAGC+TGG + chr2.3:53041097-53041116 MS.gene58707:intron 20.0%
!!! TCTTGATTAGGTTTTTTTTA+GGG + chr2.3:53039441-53039460 MS.gene58707:intron 20.0%
!!! TTCTTGATTAGGTTTTTTTT+AGG + chr2.3:53039440-53039459 MS.gene58707:intron 20.0%
! AAGGTAAAAGAAAACTATCA+AGG + chr2.3:53041719-53041738 MS.gene58707:intron 25.0%
! AAGTTAATATATATTCCCCT+TGG + chr2.3:53041838-53041857 MS.gene58707:intron 25.0%
! AATAATGATAAAATGCACAC+TGG - chr2.3:53042050-53042069 None:intergenic 25.0%
! ACATTAGAGAAAGTTTAATG+AGG + chr2.3:53039658-53039677 MS.gene58707:intron 25.0%
! AGAAAGTTATATTGGCAATT+GGG + chr2.3:53040392-53040411 MS.gene58707:CDS 25.0%
! AGATGAAAATAAGCTGAAAA+CGG + chr2.3:53039755-53039774 MS.gene58707:intron 25.0%
! CACTAAATCCAATTATCAAA+TGG - chr2.3:53041024-53041043 None:intergenic 25.0%
! CATTAGAGAAAGTTTAATGA+GGG + chr2.3:53039659-53039678 MS.gene58707:intron 25.0%
! TAAATTGAAGTGATTGTTGT+AGG + chr2.3:53040069-53040088 MS.gene58707:intron 25.0%
! TCAAGATCACAAAAATGAAA+TGG - chr2.3:53041132-53041151 None:intergenic 25.0%
! TTATGCTCGATTTAAATTTG+TGG + chr2.3:53041324-53041343 MS.gene58707:intron 25.0%
!! AGGTAAAATTTCAGGTAATT+TGG - chr2.3:53041240-53041259 None:intergenic 25.0%
!! ATTCAGAGACTACATAATTT+TGG - chr2.3:53041372-53041391 None:intergenic 25.0%
!! GGTAAAATTTCAGGTAATTT+GGG - chr2.3:53041239-53041258 None:intergenic 25.0%
!! TATATACTACTGTTGCTATT+CGG + chr2.3:53040154-53040173 MS.gene58707:intron 25.0%
!!! AAGACATTCTTGATTTGTTA+TGG + chr2.3:53039945-53039964 MS.gene58707:intron 25.0%
!!! ACTATTTTTGTTCATTGTCA+CGG - chr2.3:53040916-53040935 None:intergenic 25.0%
!!! AGACATTCTTGATTTGTTAT+GGG + chr2.3:53039946-53039965 MS.gene58707:intron 25.0%
!!! AGGTTTTTTTTAGGGTAATT+AGG + chr2.3:53039449-53039468 MS.gene58707:intron 25.0%
!!! ATGTTTTTTGCTTTTTGTTC+CGG + chr2.3:53040210-53040229 MS.gene58707:intron 25.0%
!!! CAATTTTGTTATTTGTTGAC+AGG + chr2.3:53040320-53040339 MS.gene58707:intron 25.0%
!!! CATTAAGCAATTTTTTGTTG+CGG + chr2.3:53041604-53041623 MS.gene58707:CDS 25.0%
!!! GGTTTTTTTTAGGGTAATTA+GGG + chr2.3:53039450-53039469 MS.gene58707:intron 25.0%
!!! TATCGTTTGTTATGAAAACA+GGG - chr2.3:53039692-53039711 None:intergenic 25.0%
!!! TCATTTTTTTAATACCGTGT+AGG + chr2.3:53041391-53041410 MS.gene58707:intron 25.0%
!!! TGAGATTGATTTTCTTGATT+AGG + chr2.3:53039429-53039448 MS.gene58707:intron 25.0%
!!! TTATCGTTTGTTATGAAAAC+AGG - chr2.3:53039693-53039712 None:intergenic 25.0%
!!! TTTTTGTGATATGGATTATG+TGG + chr2.3:53039618-53039637 MS.gene58707:intron 25.0%
AAAACACAATGACAAAGATG+AGG - chr2.3:53040788-53040807 None:intergenic 30.0%
AAACACAATGACAAAGATGA+GGG - chr2.3:53040787-53040806 None:intergenic 30.0%
ACAGACTGAGAAAGTTATAT+TGG + chr2.3:53040384-53040403 MS.gene58707:CDS 30.0%
AGAATCTTATCACTTCACTT+AGG - chr2.3:53041163-53041182 None:intergenic 30.0%
CACACTTGAGTTATAAAATG+AGG - chr2.3:53041260-53041279 None:intergenic 30.0%
CAGATGATTAAAAACATCTC+CGG - chr2.3:53040232-53040251 None:intergenic 30.0%
GAAAAAATAAGCAGCTACAT+TGG - chr2.3:53041086-53041105 None:intergenic 30.0%
GAAAGTTATATTGGCAATTG+GGG + chr2.3:53040393-53040412 MS.gene58707:CDS 30.0%
GAATATAACAGCTTGAGTAA+TGG - chr2.3:53041684-53041703 None:intergenic 30.0%
GAGAAAGTTATATTGGCAAT+TGG + chr2.3:53040391-53040410 MS.gene58707:CDS 30.0%
TAAAACAAACTAAGGTGACA+GGG + chr2.3:53040846-53040865 MS.gene58707:intron 30.0%
TCAAATAAGTCAATCCAAAC+AGG + chr2.3:53039852-53039871 MS.gene58707:intron 30.0%
TTAAAACAAACTAAGGTGAC+AGG + chr2.3:53040845-53040864 MS.gene58707:intron 30.0%
! ACTTATTTGAGGTTATCTAC+TGG - chr2.3:53039842-53039861 None:intergenic 30.0%
! AGTAAAAAATCAGAAAGCAC+AGG - chr2.3:53041546-53041565 None:intergenic 30.0%
! ATAGCACATTTTGTCTGAAT+TGG - chr2.3:53040970-53040989 None:intergenic 30.0%
! ATTGATAACAACCGATGTTT+GGG + chr2.3:53041988-53042007 MS.gene58707:CDS 30.0%
! GTAAAAAATCAGAAAGCACA+GGG - chr2.3:53041545-53041564 None:intergenic 30.0%
! TAGTGTTCCCATTTGATAAT+TGG + chr2.3:53041013-53041032 MS.gene58707:intron 30.0%
! TATTGATAACAACCGATGTT+TGG + chr2.3:53041987-53042006 MS.gene58707:CDS 30.0%
! TGTTTGGATTGACTTATTTG+AGG - chr2.3:53039853-53039872 None:intergenic 30.0%
! TTCATCTGTTTTCAGCTTAT+AGG - chr2.3:53039742-53039761 None:intergenic 30.0%
!! ATTTGTTGATTAGGGTTTTC+AGG + chr2.3:53039565-53039584 MS.gene58707:intron 30.0%
!! GAGGTTGAATTTGTTGATTA+GGG + chr2.3:53039557-53039576 MS.gene58707:intron 30.0%
!! GCATTTGGTTTGTTAATGAA+TGG + chr2.3:53039983-53040002 MS.gene58707:intron 30.0%
!! TGAGGTTGAATTTGTTGATT+AGG + chr2.3:53039556-53039575 MS.gene58707:intron 30.0%
!! TTTGTTGATTAGGGTTTTCA+GGG + chr2.3:53039566-53039585 MS.gene58707:intron 30.0%
!!! AATTTTTTGTTGCGGTTGAA+AGG + chr2.3:53041612-53041631 MS.gene58707:CDS 30.0%
!!! AGGGTTAGTTAGTTTTTTGT+AGG + chr2.3:53040865-53040884 MS.gene58707:intron 30.0%
!!! ATTTTTTGTTGCGGTTGAAA+GGG + chr2.3:53041613-53041632 MS.gene58707:CDS 30.0%
!!! GTATTGGTCTTTTTGTGATA+TGG + chr2.3:53039609-53039628 MS.gene58707:intron 30.0%
!!! TAGGGTTTTTGAGTTAGTAT+TGG + chr2.3:53039593-53039612 MS.gene58707:intron 30.0%
!!! TGTGCTTTCTGATTTTTTAC+TGG + chr2.3:53041545-53041564 MS.gene58707:intron 30.0%
AAAGCGTGATGAATTGACAT+TGG + chr2.3:53041496-53041515 MS.gene58707:CDS 35.0%
AAATAAGCAGCTACATTGGT+TGG - chr2.3:53041082-53041101 None:intergenic 35.0%
AAGCATTTGTGAGACAGTTT+GGG - chr2.3:53039816-53039835 None:intergenic 35.0%
AAGGTTTCTCAAATCCGTAT+TGG - chr2.3:53039190-53039209 None:intergenic 35.0%
AATCTACCATACTGATCCTA+CGG - chr2.3:53041747-53041766 None:intergenic 35.0%
ACAAACATCACTTCAGTAAC+CGG - chr2.3:53041305-53041324 None:intergenic 35.0%
ACATCCCTTCCTTGAATAAT+CGG - chr2.3:53039239-53039258 None:intergenic 35.0%
AGAAAGTTTAATGAGGGACT+TGG + chr2.3:53039665-53039684 MS.gene58707:intron 35.0%
ATATATATTCCCCTTGGAAC+AGG + chr2.3:53041844-53041863 MS.gene58707:intron 35.0%
ATCGGTTGTTATCAATACTC+GGG - chr2.3:53041984-53042003 None:intergenic 35.0%
ATGAGCCAAATCCAAATACA+AGG - chr2.3:53039878-53039897 None:intergenic 35.0%
GAAAGTTTAATGAGGGACTT+GGG + chr2.3:53039666-53039685 MS.gene58707:intron 35.0%
GACATTAGAAAACTTGAGCA+CGG + chr2.3:53040469-53040488 MS.gene58707:CDS 35.0%
TAAGCATTTGTGAGACAGTT+TGG - chr2.3:53039817-53039836 None:intergenic 35.0%
TATAACTTTCTCAGTCTGTG+AGG - chr2.3:53040383-53040402 None:intergenic 35.0%
TCAATTCATCACGCTTTACA+AGG - chr2.3:53041493-53041512 None:intergenic 35.0%
TCTTTATCATGTCACAGACT+CGG - chr2.3:53040522-53040541 None:intergenic 35.0%
TTATATTCTGCAACACCAAG+CGG + chr2.3:53041696-53041715 MS.gene58707:CDS 35.0%
TTCATAACCTTAACGATGTG+TGG + chr2.3:53040257-53040276 MS.gene58707:intron 35.0%
TTGCTTAATGCCCTGTAATA+AGG - chr2.3:53041594-53041613 None:intergenic 35.0%
TTTAGTGACCTGACATCATA+TGG + chr2.3:53041037-53041056 MS.gene58707:intron 35.0%
! ATAAAAGACGATTTGCTTCG+TGG + chr2.3:53039158-53039177 MS.gene58707:CDS 35.0%
! CATCGGTTGTTATCAATACT+CGG - chr2.3:53041985-53042004 None:intergenic 35.0%
! TTAGGGTAATTAGGGTTTTG+AGG + chr2.3:53039458-53039477 MS.gene58707:intron 35.0%
!! CGGAGATGTTTTTAATCATC+TGG + chr2.3:53040230-53040249 MS.gene58707:intron 35.0%
!! CTTTGTCATTGTGTTTTGAG+TGG + chr2.3:53040791-53040810 MS.gene58707:intron 35.0%
!! TAGGGTTTTCAGGGTAATTA+GGG + chr2.3:53039575-53039594 MS.gene58707:intron 35.0%
!! TTAGGGTTTTCAGGGTAATT+AGG + chr2.3:53039574-53039593 MS.gene58707:intron 35.0%
!!! TTAGGGTTTTGAGGTTTGAT+TGG + chr2.3:53039467-53039486 MS.gene58707:intron 35.0%
AACTTGCCTGTTGTACATCA+AGG - chr2.3:53042021-53042040 None:intergenic 40.0%
AAGGGAAAGAGATGCCATTA+TGG + chr2.3:53041937-53041956 MS.gene58707:CDS 40.0%
AGAAATCAGGCAAACCTACA+CGG - chr2.3:53041408-53041427 None:intergenic 40.0%
AGAAGAAACTCACTCTCTGA+CGG - chr2.3:53039336-53039355 None:intergenic 40.0%
AGACAATCCTTGACACATGA+CGG - chr2.3:53040640-53040659 None:intergenic 40.0%
AGGAAGACTTCACAATGTCA+AGG + chr2.3:53040885-53040904 MS.gene58707:intron 40.0%
AGGGAAAGAGATGCCATTAT+GGG + chr2.3:53041938-53041957 MS.gene58707:CDS 40.0%
AGTTTATGACAGATCCAGTG+AGG + chr2.3:53041465-53041484 MS.gene58707:CDS 40.0%
ATCCAAATACAAGGCCTGTT+TGG - chr2.3:53039869-53039888 None:intergenic 40.0%
ATGGATTTCGAAACTACCGA+AGG + chr2.3:53039101-53039120 MS.gene58707:CDS 40.0%
CAAACATCACTTCAGTAACC+GGG - chr2.3:53041304-53041323 None:intergenic 40.0%
CTGATGAAATGTTGAGCAGA+GGG + chr2.3:53040692-53040711 MS.gene58707:CDS 40.0%
CTGCTTGCATTACAGATACT+CGG + chr2.3:53040022-53040041 MS.gene58707:intron 40.0%
CTTAATGCCCTGTAATAAGG+AGG - chr2.3:53041591-53041610 None:intergenic 40.0%
CTTCGTGGAATCTACCAATA+CGG + chr2.3:53039173-53039192 MS.gene58707:CDS 40.0%
GAAAACTATCAAGGATCCGT+AGG + chr2.3:53041728-53041747 MS.gene58707:intron 40.0%
GAGTCTGTGACATGATAAAG+AGG + chr2.3:53040521-53040540 MS.gene58707:CDS 40.0%
GTAAAGAGAACCTGAAGATC+CGG - chr2.3:53040757-53040776 None:intergenic 40.0%
GTCATCTCCAGAATTTCATG+AGG - chr2.3:53041440-53041459 None:intergenic 40.0%
TACCAGAACTAGCAACTTGA+TGG - chr2.3:53040566-53040585 None:intergenic 40.0%
TACGCTACCTCATGAAATTC+TGG + chr2.3:53041430-53041449 MS.gene58707:CDS 40.0%
TCTGATGAAATGTTGAGCAG+AGG + chr2.3:53040691-53040710 MS.gene58707:CDS 40.0%
TTCACTGTTTCATCCATGCA+CGG + chr2.3:53041902-53041921 MS.gene58707:CDS 40.0%
! ACAGGCCTTGTATTTGGATT+TGG + chr2.3:53039870-53039889 MS.gene58707:intron 40.0%
! ATCCAAACAGGCCTTGTATT+TGG + chr2.3:53039864-53039883 MS.gene58707:intron 40.0%
! GTTTTCTTTTACCTTCCGCT+TGG - chr2.3:53041714-53041733 None:intergenic 40.0%
!! TTAACGATGTGTGGTTTGCT+CGG + chr2.3:53040266-53040285 MS.gene58707:intron 40.0%
AACAACCTGACAAACCGTCA+AGG - chr2.3:53039306-53039325 None:intergenic 45.0%
AACCCTGCCTCCTTATTACA+GGG + chr2.3:53041581-53041600 MS.gene58707:intron 45.0%
AAGGATCCGTAGGATCAGTA+TGG + chr2.3:53041738-53041757 MS.gene58707:intron 45.0%
AATCGGAAGCTTCGAAGAGA+TGG + chr2.3:53039133-53039152 MS.gene58707:CDS 45.0%
ACGTGACTCCATATGATGTC+AGG - chr2.3:53041048-53041067 None:intergenic 45.0%
ACTCAAGTGTGTGCATCGTT+AGG + chr2.3:53041269-53041288 MS.gene58707:intron 45.0%
AGCACGGAATTCACCCATAA+TGG - chr2.3:53041954-53041973 None:intergenic 45.0%
AGGAAGGGATGTTATTGCTC+AGG + chr2.3:53039247-53039266 MS.gene58707:CDS 45.0%
AGTGAGTGTAGAGTAGAGTG+AGG + chr2.3:53039902-53039921 MS.gene58707:intron 45.0%
ATACAAGCCCATGCTTGCAT+TGG + chr2.3:53040427-53040446 MS.gene58707:CDS 45.0%
ATCGGAAGCTTCGAAGAGAT+GGG + chr2.3:53039134-53039153 MS.gene58707:CDS 45.0%
ATGGCATCTCTTTCCCTTTG+AGG - chr2.3:53041935-53041954 None:intergenic 45.0%
ATTATGGGTGAATTCCGTGC+TGG + chr2.3:53041953-53041972 MS.gene58707:CDS 45.0%
CTCAAGTGTGTGCATCGTTA+GGG + chr2.3:53041270-53041289 MS.gene58707:intron 45.0%
GACTTCGATGATTGCCTTGA+CGG + chr2.3:53039289-53039308 MS.gene58707:CDS 45.0%
GAGCAAACCACACATCGTTA+AGG - chr2.3:53040267-53040286 None:intergenic 45.0%
GCGTGATGAATTGACATTGG+AGG + chr2.3:53041499-53041518 MS.gene58707:CDS 45.0%
GTGCATGTGATACTGGCATT+TGG + chr2.3:53039968-53039987 MS.gene58707:intron 45.0%
GTTATGGGTGCATGTGATAC+TGG + chr2.3:53039961-53039980 MS.gene58707:intron 45.0%
TAACCCTGCCTCCTTATTAC+AGG + chr2.3:53041580-53041599 MS.gene58707:intron 45.0%
TATATTCCCCTTGGAACAGG+TGG + chr2.3:53041847-53041866 MS.gene58707:intron 45.0%
TGTGTACAGATATCTCCCAC+CGG + chr2.3:53040735-53040754 MS.gene58707:CDS 45.0%
TTCTTTGCCGTCATGTGTCA+AGG + chr2.3:53040630-53040649 MS.gene58707:intron 45.0%
TTGCATTGGAGGGAAAAGTG+TGG + chr2.3:53040441-53040460 MS.gene58707:CDS 45.0%
TTTCCCTCCAATGCAAGCAT+GGG - chr2.3:53040437-53040456 None:intergenic 45.0%
! TGCATTGGAGGGAAAAGTGT+GGG + chr2.3:53040442-53040461 MS.gene58707:CDS 45.0%
! TTTTCCCTCCAATGCAAGCA+TGG - chr2.3:53040438-53040457 None:intergenic 45.0%
! TTTTGTTGCGGTTGAAAGGG+AGG + chr2.3:53041616-53041635 MS.gene58707:CDS 45.0%
!! TGACAAAGATGAGGGCATCA+AGG - chr2.3:53040779-53040798 None:intergenic 45.0%
AACCAGTCCACCTGTTCCAA+GGG - chr2.3:53041857-53041876 None:intergenic 50.0%
AAGAGAACCTGAAGATCCGG+TGG - chr2.3:53040754-53040773 None:intergenic 50.0%
AAGCCCATGCTTGCATTGGA+GGG + chr2.3:53040431-53040450 MS.gene58707:CDS 50.0%
AGAAACTCACTCTCTGACGG+AGG - chr2.3:53039333-53039352 None:intergenic 50.0%
AGAGAACCTGAAGATCCGGT+GGG - chr2.3:53040753-53040772 None:intergenic 50.0%
ATATCTCCCACCGGATCTTC+AGG + chr2.3:53040744-53040763 MS.gene58707:CDS 50.0%
ATCGTTAGGGTCCTTGATCC+CGG + chr2.3:53041283-53041302 MS.gene58707:intron 50.0%
ATGCCCTGTAATAAGGAGGC+AGG - chr2.3:53041587-53041606 None:intergenic 50.0%
ATTCTGCAACACCAAGCGGA+AGG + chr2.3:53041700-53041719 MS.gene58707:CDS 50.0%
CACTTCAGTAACCGGGATCA+AGG - chr2.3:53041297-53041316 None:intergenic 50.0%
CGCTTTACAAGGATCCTCAC+TGG - chr2.3:53041482-53041501 None:intergenic 50.0%
GAGGTAGCGTAGCAGAAATC+AGG - chr2.3:53041421-53041440 None:intergenic 50.0%
GATTGCCTTGACGGTTTGTC+AGG + chr2.3:53039298-53039317 MS.gene58707:CDS 50.0%
GTTATTGCTCAGGCGCAATC+TGG + chr2.3:53039257-53039276 MS.gene58707:CDS 50.0%
TAACCAGTCCACCTGTTCCA+AGG - chr2.3:53041858-53041877 None:intergenic 50.0%
TCCGATTGCTTTCACGCCTT+CGG - chr2.3:53039120-53039139 None:intergenic 50.0%
TCGGAAGCTTCGAAGAGATG+GGG + chr2.3:53039135-53039154 MS.gene58707:CDS 50.0%
TGCCCTGTAATAAGGAGGCA+GGG - chr2.3:53041586-53041605 None:intergenic 50.0%
TGTGAGGCCAGTTCTCTTGT+CGG - chr2.3:53040367-53040386 None:intergenic 50.0%
TGTGTCTCCGACAAGAGAAC+TGG + chr2.3:53040357-53040376 MS.gene58707:CDS 50.0%
TTGCGGTTGAAAGGGAGGAA+TGG + chr2.3:53041621-53041640 MS.gene58707:CDS 50.0%
! AACTAGCAACTTGATGGCCC+TGG - chr2.3:53040560-53040579 None:intergenic 50.0%
! ACCGAAGGCGTGAAAGCAAT+CGG + chr2.3:53039116-53039135 MS.gene58707:CDS 50.0%
! ATACTCGGGTTGTTCCAGCA+CGG - chr2.3:53041970-53041989 None:intergenic 50.0%
! GGCCATCAAGTTGCTAGTTC+TGG + chr2.3:53040561-53040580 MS.gene58707:CDS 50.0%
! TCGTGGCCTTGATGTACAAC+AGG + chr2.3:53042012-53042031 MS.gene58707:CDS 50.0%
!! GGCGCCGATTATTCAAGGAA+GGG + chr2.3:53039232-53039251 MS.gene58707:CDS 50.0%
!! TGGCGCCGATTATTCAAGGA+AGG + chr2.3:53039231-53039250 MS.gene58707:CDS 50.0%
AAGGCCACGAGCCCAAACAT+CGG - chr2.3:53042002-53042021 None:intergenic 55.0%
ACAACCGATGTTTGGGCTCG+TGG + chr2.3:53041995-53042014 MS.gene58707:CDS 55.0%
ACCAGTCCACCTGTTCCAAG+GGG - chr2.3:53041856-53041875 None:intergenic 55.0%
AGAGGAGAACATTGCGCACC+AGG + chr2.3:53040539-53040558 MS.gene58707:CDS 55.0%
ATCATGTCACAGACTCGGCC+AGG - chr2.3:53040517-53040536 None:intergenic 55.0%
CAAGCCCATGCTTGCATTGG+AGG + chr2.3:53040430-53040449 MS.gene58707:CDS 55.0%
CACGGAGTTCATGTCGTGTC+TGG + chr2.3:53040487-53040506 MS.gene58707:CDS 55.0%
GAATCGCCGACGAACGGTTA+CGG + chr2.3:53039067-53039086 MS.gene58707:CDS 55.0%
GAGGAGAACATTGCGCACCA+GGG + chr2.3:53040540-53040559 MS.gene58707:CDS 55.0%
GCACGCTGTTGAATCGCAGA+AGG - chr2.3:53039209-53039228 None:intergenic 55.0%
GCACGGTGACATGCCTCAAA+GGG + chr2.3:53041919-53041938 MS.gene58707:CDS 55.0%
TGCACGGTGACATGCCTCAA+AGG + chr2.3:53041918-53041937 MS.gene58707:CDS 55.0%
TGCGATTCAACAGCGTGCTG+TGG + chr2.3:53039211-53039230 MS.gene58707:CDS 55.0%
TTGAGGCATGTCACCGTGCA+TGG - chr2.3:53041918-53041937 None:intergenic 55.0%
! CATGTCGTGTCTGGAACTCC+TGG + chr2.3:53040496-53040515 MS.gene58707:CDS 55.0%
!! GCTGTGGCGCCGATTATTCA+AGG + chr2.3:53039227-53039246 MS.gene58707:CDS 55.0%
ACGAACGGTTACGGCGAACG+AGG + chr2.3:53039076-53039095 MS.gene58707:CDS 60.0%
CGTTCGCCGTAACCGTTCGT+CGG - chr2.3:53039076-53039095 None:intergenic 60.0%
GCTCAGGCGCAATCTGGTAC+TGG + chr2.3:53039263-53039282 MS.gene58707:CDS 60.0%
GGCGACAGCTTCTGTGGTTC+CGG + chr2.3:53039043-53039062 MS.gene58707:CDS 60.0%
GGTTACGGCGAACGAGGACA+TGG + chr2.3:53039082-53039101 MS.gene58707:CDS 60.0%
TCCCCTTGGAACAGGTGGAC+TGG + chr2.3:53041852-53041871 MS.gene58707:intron 60.0%
ACCGTTCGTCGGCGATTCGC+CGG - chr2.3:53039065-53039084 None:intergenic 65.0%
TCCGGCGAATCGCCGACGAA+CGG + chr2.3:53039061-53039080 MS.gene58707:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 53039041 53042093 53039041 ID=MS.gene58707
chr2.3 mRNA 53039041 53042093 53039041 ID=MS.gene58707.t1;Parent=MS.gene58707
chr2.3 exon 53039041 53039342 53039041 ID=MS.gene58707.t1.exon1;Parent=MS.gene58707.t1
chr2.3 CDS 53039041 53039342 53039041 ID=cds.MS.gene58707.t1;Parent=MS.gene58707.t1
chr2.3 exon 53040342 53040582 53040342 ID=MS.gene58707.t1.exon2;Parent=MS.gene58707.t1
chr2.3 CDS 53040342 53040582 53040342 ID=cds.MS.gene58707.t1;Parent=MS.gene58707.t1
chr2.3 exon 53040685 53040765 53040685 ID=MS.gene58707.t1.exon3;Parent=MS.gene58707.t1
chr2.3 CDS 53040685 53040765 53040685 ID=cds.MS.gene58707.t1;Parent=MS.gene58707.t1
chr2.3 exon 53041413 53041520 53041413 ID=MS.gene58707.t1.exon4;Parent=MS.gene58707.t1
chr2.3 CDS 53041413 53041520 53041413 ID=cds.MS.gene58707.t1;Parent=MS.gene58707.t1
chr2.3 exon 53041602 53041721 53041602 ID=MS.gene58707.t1.exon5;Parent=MS.gene58707.t1
chr2.3 CDS 53041602 53041721 53041602 ID=cds.MS.gene58707.t1;Parent=MS.gene58707.t1
chr2.3 exon 53041866 53042093 53041866 ID=MS.gene58707.t1.exon6;Parent=MS.gene58707.t1
chr2.3 CDS 53041866 53042093 53041866 ID=cds.MS.gene58707.t1;Parent=MS.gene58707.t1
Gene Sequence

>MS.gene58707

ATGGCGACAGCTTCTGTGGTTCCGGCGAATCGCCGACGAACGGTTACGGCGAACGAGGACATGGATTTCGAAACTACCGAAGGCGTGAAAGCAATCGGAAGCTTCGAAGAGATGGGGATAAAAGACGATTTGCTTCGTGGAATCTACCAATACGGATTTGAGAAACCTTCTGCGATTCAACAGCGTGCTGTGGCGCCGATTATTCAAGGAAGGGATGTTATTGCTCAGGCGCAATCTGGTACTGGAAAGACTTCGATGATTGCCTTGACGGTTTGTCAGGTTGTTGATACCTCCGTCAGAGAGTGAGTTTCTTCTTCAATCTTGCTTTTGATTTTTGTTGTGTTAGTTAATTTGTTTGAATTTGGAATTTGAAGAGTAGAGATTAATTTGAGATTGATTTTCTTGATTAGGTTTTTTTTAGGGTAATTAGGGTTTTGAGGTTTGATTGGTGTTTGTTTTATGTTAATTTGTTTGGATAAACAATTGGAAGAGAAAAGTATAAATAGAGAGTAATTTGAGGTTGAATTTGTTGATTAGGGTTTTCAGGGTAATTAGGGTTTTTGAGTTAGTATTGGTCTTTTTGTGATATGGATTATGTGGATTTAAGAATTATGAAGACATTAGAGAAAGTTTAATGAGGGACTTGGGCCCTGTTTTCATAACAAACGATAAATTAAAGTCAAACTGTTTTTAATTAACCTATAAGCTGAAAACAGATGAAAATAAGCTGAAAACGGCTCATGAACATGTCATAAGTCGTTACATAAGATGTCCCAAACTGTCTCACAAATGCTTATGCCAGTAGATAACCTCAAATAAGTCAATCCAAACAGGCCTTGTATTTGGATTTGGCTCATCTAGAGTGAGTGTAGAGTAGAGTGAGGAAGTCAATGATTTATATTTCAAGACATTCTTGATTTGTTATGGGTGCATGTGATACTGGCATTTGGTTTGTTAATGAATGGTTAATAACTTAATAAGCTGCTTGCATTACAGATACTCGGAATTATTTTTTTATTTTATTTTTTTAAATTGAAGTGATTGTTGTAGGAATTGATGATTATTTTTAGAGTAGAAGTAATAAAATGATTATTATTTGTTGTTTGTATAGACTATATACTACTGTTGCTATTCGGATATAGTTCTTTTAATTTATAAATGGAAATCTGATGTTTTTTGCTTTTTGTTCCGGAGATGTTTTTAATCATCTGGTATATTCATAACCTTAACGATGTGTGGTTTGCTCGGAACAAAGTACATCAGTTTATTAATTTGTGTTCAATTTTGTTATTTGTTGACAGGGTGCAAGCTTTAATTGTGTCTCCGACAAGAGAACTGGCCTCACAGACTGAGAAAGTTATATTGGCAATTGGGGATTTTATCAATATACAAGCCCATGCTTGCATTGGAGGGAAAAGTGTGGGAGAAGACATTAGAAAACTTGAGCACGGAGTTCATGTCGTGTCTGGAACTCCTGGCCGAGTCTGTGACATGATAAAGAGGAGAACATTGCGCACCAGGGCCATCAAGTTGCTAGTTCTGGTAATTAATTGCTTCTAATTGCTGATGCTCGATTGTATGTTTTTTTCTTCTTTGCCGTCATGTGTCAAGGATTGTCTCATATATTTTTCTTTTCAAATACAGGATGAATCTGATGAAATGTTGAGCAGAGGGTTTAAAGATCAGATTTATGATGTGTACAGATATCTCCCACCGGATCTTCAGGTTCTCTTTACCTTGATGCCCTCATCTTTGTCATTGTGTTTTGAGTGGAATTTCTGCAATTTGAAATCTTATTATATGCTTAAAACAAACTAAGGTGACAGGGTTAGTTAGTTTTTTGTAGGAAGACTTCACAATGTCAAGGATTTACCGTGACAATGAACAAAAATAGTATTGAATATTGGATTTTAGACATAACAATTTCCAATTCAGACAAAATGTGCTATTAAATAAGGATATAAGAGGTTTATAGTGTTCCCATTTGATAATTGGATTTAGTGACCTGACATCATATGGAGTCACGTTAATGTTTCAACCAACCAATGTAGCTGCTTATTTTTTCGTAATATAAGCTGGTAGCAGTAACCATTTCATTTTTGTGATCTTGAATGAAGAACCTAAGTGAAGTGATAAGATTCTGATACAGATTTTTATTAATATGTTTTCTTTATTTGGTTTTCAAACTTATGTCACCCAAATTACCTGAAATTTTACCTCATTTTATAACTCAAGTGTGTGCATCGTTAGGGTCCTTGATCCCGGTTACTGAAGTGATGTTTGTTATGCTCGATTTAAATTTGTGGAACAGTTTTGTCTTTATTCTTTCCAAAATTATGTAGTCTCTGAATCATTTTTTTAATACCGTGTAGGTTTGCCTGATTTCTGCTACGCTACCTCATGAAATTCTGGAGATGACAAACAAGTTTATGACAGATCCAGTGAGGATCCTTGTAAAGCGTGATGAATTGACATTGGAGGTAAGCAATTGCTTTTATTGTCCCTGTGCTTTCTGATTTTTTACTGGATATTGATACAATAACCCTGCCTCCTTATTACAGGGCATTAAGCAATTTTTTGTTGCGGTTGAAAGGGAGGAATGGAAGTTTGATACTCTGTGTGATCTATATGATACTCTCACCATTACTCAAGCTGTTATATTCTGCAACACCAAGCGGAAGGTAAAAGAAAACTATCAAGGATCCGTAGGATCAGTATGGTAGATTATTAGATTCGATACTCTATATTTGCACATGTTGATCAGTTTACAGTTTTTATCTTATCATTATATTGATTCAAGTTAATATATATTCCCCTTGGAACAGGTGGACTGGTTAACTGAAAAAATGCGTAACAATAATTTCACTGTTTCATCCATGCACGGTGACATGCCTCAAAGGGAAAGAGATGCCATTATGGGTGAATTCCGTGCTGGAACAACCCGAGTATTGATAACAACCGATGTTTGGGCTCGTGGCCTTGATGTACAACAGGCAAGTTATCTGTCCAGTGTGCATTTTATCATTATTATTATTTGTGCCTCTAAAATATGA
Protein sequence

>MS.gene58707.t1

MATASVVPANRRRTVTANEDMDFETTEGVKAIGSFEEMGIKDDLLRGIYQYGFEKPSAIQQRAVAPIIQGRDVIAQAQSGTGKTSMIALTVCQVVDTSVREVQALIVSPTRELASQTEKVILAIGDFINIQAHACIGGKSVGEDIRKLEHGVHVVSGTPGRVCDMIKRRTLRTRAIKLLVLDESDEMLSRGFKDQIYDVYRYLPPDLQVCLISATLPHEILEMTNKFMTDPVRILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTEKMRNNNFTVSSMHGDMPQRERDAIMGEFRAGTTRVLITTDVWARGLDVQQASYLSSVHFIIIIICASKI