Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58777.t1 | AES91696.2 | 95.4 | 715 | 30 | 2 | 1 | 714 | 9 | 721 | 0.00E+00 | 1267.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58777.t1 | D5LXJ0 | 73.0 | 718 | 162 | 8 | 1 | 714 | 1 | 690 | 3.9e-269 | 928.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58777.t1 | G7JGN6 | 95.4 | 715 | 30 | 2 | 1 | 714 | 9 | 721 | 0.0e+00 | 1267.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050527 | MS.gene58777 | -0.805899 | 1.07E-49 | -1.69E-46 |
| MS.gene050623 | MS.gene58777 | 0.816275 | 6.01E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58777.t1 | MTR_4g116370 | 96.643 | 715 | 21 | 2 | 1 | 714 | 9 | 721 | 0.0 | 1366 |
| MS.gene58777.t1 | MTR_4g116370 | 96.643 | 715 | 21 | 2 | 1 | 714 | 1 | 713 | 0.0 | 1365 |
| MS.gene58777.t1 | MTR_4g116370 | 94.798 | 692 | 31 | 3 | 26 | 714 | 10 | 699 | 0.0 | 1287 |
| MS.gene58777.t1 | MTR_4g116370 | 95.908 | 611 | 22 | 2 | 105 | 714 | 17 | 625 | 0.0 | 1145 |
| MS.gene58777.t1 | MTR_4g116370 | 95.596 | 545 | 21 | 2 | 171 | 714 | 1 | 543 | 0.0 | 997 |
| MS.gene58777.t1 | MTR_1g026290 | 34.630 | 514 | 266 | 16 | 19 | 504 | 24 | 495 | 4.55e-64 | 229 |
| MS.gene58777.t1 | MTR_1g026290 | 34.630 | 514 | 266 | 16 | 19 | 504 | 24 | 495 | 5.77e-64 | 229 |
| MS.gene58777.t1 | MTR_1g026290 | 34.630 | 514 | 266 | 16 | 19 | 504 | 24 | 495 | 1.10e-63 | 229 |
| MS.gene58777.t1 | MTR_1g026290 | 34.630 | 514 | 266 | 16 | 19 | 504 | 24 | 495 | 1.44e-63 | 229 |
| MS.gene58777.t1 | MTR_1g026290 | 34.629 | 283 | 156 | 10 | 228 | 504 | 15 | 274 | 3.01e-30 | 127 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58777.t1 | AT5G25270 | 38.710 | 682 | 317 | 19 | 1 | 672 | 1 | 591 | 6.00e-110 | 347 |
| MS.gene58777.t1 | AT5G42220 | 34.521 | 449 | 256 | 10 | 11 | 435 | 13 | 447 | 1.01e-57 | 211 |
| MS.gene58777.t1 | AT5G42220 | 34.521 | 449 | 256 | 10 | 11 | 435 | 13 | 447 | 1.01e-57 | 211 |
| MS.gene58777.t1 | AT5G11080 | 52.564 | 78 | 36 | 1 | 22 | 98 | 11 | 88 | 4.57e-16 | 80.5 |
| MS.gene58777.t1 | AT5G11080 | 31.210 | 157 | 91 | 2 | 227 | 383 | 123 | 262 | 3.76e-14 | 74.7 |
| MS.gene58777.t1 | AT5G11080 | 52.564 | 78 | 36 | 1 | 22 | 98 | 11 | 88 | 6.18e-16 | 80.5 |
| MS.gene58777.t1 | AT5G11080 | 31.210 | 157 | 91 | 2 | 227 | 383 | 123 | 262 | 6.35e-14 | 74.3 |
| MS.gene58777.t1 | AT5G11080 | 31.210 | 157 | 91 | 2 | 227 | 383 | 144 | 283 | 1.41e-13 | 73.6 |
| MS.gene58777.t1 | AT5G11080 | 41.414 | 99 | 36 | 2 | 22 | 98 | 11 | 109 | 7.31e-12 | 68.2 |
Find 205 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGTTCAGCTTCAGGGGCTT+GGG | 0.189395 | 8.3:+9539020 | None:intergenic |
| TCACCACTACTTGCAAAATT+TGG | 0.191628 | 8.3:+9542531 | None:intergenic |
| AACATTCAACGTTCCTATTC+AGG | 0.214574 | 8.3:-9542702 | MS.gene58777:CDS |
| AAATATACCCTATTTATTTC+AGG | 0.218452 | 8.3:+9542665 | None:intergenic |
| CTATGGCTTGTGCTTGAATT+TGG | 0.218642 | 8.3:+9542761 | None:intergenic |
| GTATCTGTCTATCAGATTCT+TGG | 0.233942 | 8.3:+9539169 | None:intergenic |
| TGCCTTTGCAATGTGGATTC+AGG | 0.236984 | 8.3:+9539786 | None:intergenic |
| TAGAATCTCAGGAAGGTAAA+AGG | 0.241582 | 8.3:+9538417 | None:intergenic |
| ACTTCCGGCGTCAGTTTCTC+TGG | 0.257615 | 8.3:-9541416 | MS.gene58777:CDS |
| GCATGATTGGGCAGACTTCC+TGG | 0.260161 | 8.3:+9544871 | None:intergenic |
| GAGTTCCACCTGAAATAAAT+AGG | 0.264479 | 8.3:-9542673 | MS.gene58777:CDS |
| CAGGTTCAGCTTCAGGGGCT+TGG | 0.267729 | 8.3:+9539019 | None:intergenic |
| GGGCTTTACCATCCCGTTCT+TGG | 0.275600 | 8.3:-9539897 | MS.gene58777:CDS |
| GATAGATTACGGAATACTTT+TGG | 0.275889 | 8.3:-9541439 | MS.gene58777:CDS |
| GTGTATCCCCACGCTCTTCT+TGG | 0.276948 | 8.3:+9540099 | None:intergenic |
| GGGATCTGGTTCTTCACGTT+TGG | 0.283504 | 8.3:-9541038 | MS.gene58777:CDS |
| GCATCTATTGCTGGAGAATC+TGG | 0.286152 | 8.3:-9540008 | MS.gene58777:CDS |
| ACGCTCTTCTTGGCTGGCAT+TGG | 0.292527 | 8.3:+9540109 | None:intergenic |
| CGACTTGAGTACTGTGACTA+TGG | 0.296537 | 8.3:+9542744 | None:intergenic |
| CGCTCTTCTTGGCTGGCATT+GGG | 0.305234 | 8.3:+9540110 | None:intergenic |
| CTAACAGCAGGTTCAGCTTC+AGG | 0.317466 | 8.3:+9539012 | None:intergenic |
| TGTTGTAAATATCAATATTC+TGG | 0.326053 | 8.3:-9539214 | MS.gene58777:CDS |
| GAACAGATTGCTACTGTAAC+TGG | 0.331567 | 8.3:-9545745 | MS.gene58777:CDS |
| GATGGCTCTTGTCTTGTGTT+TGG | 0.340573 | 8.3:+9539246 | None:intergenic |
| TTGCATCAAACCTGCATGAT+TGG | 0.341528 | 8.3:+9544858 | None:intergenic |
| GGATCTGGTTCTTCACGTTT+GGG | 0.343525 | 8.3:-9541037 | MS.gene58777:CDS |
| TCCTAATCATATCATGGTTC+AGG | 0.344402 | 8.3:-9540437 | MS.gene58777:intron |
| GGCTTTACCATCCCGTTCTT+GGG | 0.345140 | 8.3:-9539896 | MS.gene58777:CDS |
| TGCAGCAGTACCTGGTCCCT+TGG | 0.347139 | 8.3:-9539955 | MS.gene58777:CDS |
| CCGGTCATATCTCAACAAAT+AGG | 0.355024 | 8.3:-9538946 | MS.gene58777:CDS |
| TTTCAAGCTGGAGCAAGCTT+TGG | 0.360970 | 8.3:-9540332 | MS.gene58777:CDS |
| CCGAAGTTCTTCCAAGGCCT+TGG | 0.364532 | 8.3:+9541527 | None:intergenic |
| TGCGATCAACCACTCAATCT+AGG | 0.370044 | 8.3:-9540685 | MS.gene58777:CDS |
| TTGCTGGAGAATCTGGAATA+AGG | 0.374237 | 8.3:-9540001 | MS.gene58777:CDS |
| TACTCTCAAAGTGGATAAAC+AGG | 0.377859 | 8.3:-9547385 | MS.gene58777:intron |
| AGACATAGTGGTTCTGAATC+AGG | 0.384452 | 8.3:-9538470 | MS.gene58777:CDS |
| TACAACCATGACATTGCGTT+TGG | 0.388304 | 8.3:-9540603 | MS.gene58777:CDS |
| CTTCCGGCGTCAGTTTCTCT+GGG | 0.397182 | 8.3:-9541415 | MS.gene58777:CDS |
| TACTATGAGCCTCAGCTGCT+TGG | 0.398831 | 8.3:+9541071 | None:intergenic |
| CAGTCTGAAGCTGTAGTTAA+TGG | 0.401358 | 8.3:-9540490 | MS.gene58777:CDS |
| AGTCGTGAATTTGATACAAT+TGG | 0.403251 | 8.3:-9541208 | MS.gene58777:intron |
| TTTACAACACGAGAATTAGA+TGG | 0.404121 | 8.3:+9539228 | None:intergenic |
| CGTCCGGATGCATCTATTGC+TGG | 0.406144 | 8.3:-9540017 | MS.gene58777:CDS |
| GAACCATCTGGTAATTCTAC+TGG | 0.406393 | 8.3:-9539918 | MS.gene58777:CDS |
| GTGGTGAAGGGATTGTTGTC+AGG | 0.408467 | 8.3:-9542515 | MS.gene58777:CDS |
| GCACAGACTGACTCAAGTTT+AGG | 0.410476 | 8.3:-9540290 | MS.gene58777:CDS |
| TGCATCAAACCTGCATGATT+GGG | 0.410770 | 8.3:+9544859 | None:intergenic |
| GGAACTCCATCGCCCTGAAT+AGG | 0.417360 | 8.3:+9542689 | None:intergenic |
| CTTCCTGGAGGTGGTAGATC+AGG | 0.417988 | 8.3:+9544886 | None:intergenic |
| CGGTCATATCTCAACAAATA+GGG | 0.421449 | 8.3:-9538945 | MS.gene58777:CDS |
| TTCTTGGCTGGCATTGGGTG+TGG | 0.423169 | 8.3:+9540115 | None:intergenic |
| TCAGTCTGTGCAGCTCCTAC+AGG | 0.423837 | 8.3:+9540302 | None:intergenic |
| AGGGCAGGCAAATAGTGAAC+AGG | 0.424813 | 8.3:-9539853 | MS.gene58777:CDS |
| TGACTAGCAGAACTTTCAAC+TGG | 0.426756 | 8.3:+9540053 | None:intergenic |
| GACTCATATGACTTAGGTAC+TGG | 0.430640 | 8.3:+9541227 | None:intergenic |
| GGTTCAGCTTCAGGGGCTTG+GGG | 0.431903 | 8.3:+9539021 | None:intergenic |
| AAGGTAAAAGGGTAACCTTT+TGG | 0.434045 | 8.3:+9538429 | None:intergenic |
| ATCCCCACGCTCTTCTTGGC+TGG | 0.442443 | 8.3:+9540103 | None:intergenic |
| GAGCATGATTCCCAAGGCCT+TGG | 0.443263 | 8.3:-9541538 | MS.gene58777:CDS |
| CGAAGTTCTTCCAAGGCCTT+GGG | 0.443435 | 8.3:+9541528 | None:intergenic |
| CGTCCCCTACTTGATCAAAC+TGG | 0.449051 | 8.3:-9541478 | MS.gene58777:CDS |
| ACCTGAACCATGATATGATT+AGG | 0.449353 | 8.3:+9540436 | None:intergenic |
| TGTTCTTTCAAAGCAGGGAC+TGG | 0.450476 | 8.3:+9545763 | None:intergenic |
| ACTATGAGCCTCAGCTGCTT+GGG | 0.454818 | 8.3:+9541072 | None:intergenic |
| ATCTGTATATCGATACGCCT+TGG | 0.455517 | 8.3:+9540242 | None:intergenic |
| TCTTCCATCACCCGCATCCT+TGG | 0.457037 | 8.3:-9540999 | MS.gene58777:CDS |
| GCTGCAACCATGGTTCTAAT+CGG | 0.458329 | 8.3:+9539972 | None:intergenic |
| ACAACCATGACATTGCGTTT+GGG | 0.460634 | 8.3:-9540602 | MS.gene58777:CDS |
| GAATGGATCTGATGCTCCAC+TGG | 0.462859 | 8.3:+9541501 | None:intergenic |
| GCAATCTGTTCTTTCAAAGC+AGG | 0.463654 | 8.3:+9545757 | None:intergenic |
| TTTGAGCTTTATCTCAATAG+TGG | 0.465150 | 8.3:+9547428 | None:intergenic |
| TTGCCAAGGGAAAGTTCTAA+AGG | 0.470571 | 8.3:-9545695 | MS.gene58777:CDS |
| TAACAGCAGGTTCAGCTTCA+GGG | 0.470691 | 8.3:+9539013 | None:intergenic |
| CAATGCATGCTGCAACATCA+AGG | 0.471454 | 8.3:-9539050 | MS.gene58777:CDS |
| CACAGTACTCAAGTCGCTCC+AGG | 0.472501 | 8.3:-9542737 | MS.gene58777:CDS |
| AGAATCTCAGGAAGGTAAAA+GGG | 0.474369 | 8.3:+9538418 | None:intergenic |
| GCAGCAGTACCTGGTCCCTT+GGG | 0.475180 | 8.3:-9539954 | MS.gene58777:CDS |
| CTTCCAGTGCTGTTATTGCT+AGG | 0.475329 | 8.3:+9547459 | None:intergenic |
| AGTAATGAAGCAATGGGATC+TGG | 0.476060 | 8.3:-9541052 | MS.gene58777:CDS |
| TCTGTATATCGATACGCCTT+GGG | 0.477521 | 8.3:+9540243 | None:intergenic |
| AGTTCCACCTGAAATAAATA+GGG | 0.478302 | 8.3:-9542672 | MS.gene58777:intron |
| ATCAATATTCTGGCAGCTGG+TGG | 0.478719 | 8.3:-9539204 | MS.gene58777:CDS |
| CGTTGAAATCTCCCAAGAAC+GGG | 0.478770 | 8.3:+9539885 | None:intergenic |
| AACGTTCCTATTCAGGGCGA+TGG | 0.482883 | 8.3:-9542695 | MS.gene58777:CDS |
| CAATCTAGGGCATTGAGGAC+TGG | 0.486555 | 8.3:-9540671 | MS.gene58777:CDS |
| AAGCTTGCTCCAGCTTGAAA+AGG | 0.487152 | 8.3:+9540335 | None:intergenic |
| TGAACCAGTTTGATCAAGTA+GGG | 0.490565 | 8.3:+9541474 | None:intergenic |
| CCAAGGCCTTGGAAGAACTT+CGG | 0.491774 | 8.3:-9541527 | MS.gene58777:CDS |
| AGATTTCAACGTGTAACTAC+AGG | 0.493475 | 8.3:-9539873 | MS.gene58777:CDS |
| ACATTCAACGTTCCTATTCA+GGG | 0.496989 | 8.3:-9542701 | MS.gene58777:CDS |
| CGTGCTTCTTCAGAACCATC+TGG | 0.498068 | 8.3:-9539930 | MS.gene58777:CDS |
| ATGTAGGTTGAAACTAATGC+TGG | 0.498291 | 8.3:-9538503 | MS.gene58777:intron |
| AAGACTTCTGCCAAGGATGC+GGG | 0.502779 | 8.3:+9540989 | None:intergenic |
| TCAAGCAACCTCTAGGCGTC+CGG | 0.504573 | 8.3:-9540033 | MS.gene58777:CDS |
| CATCCTTTAGAACTTTCCCT+TGG | 0.506283 | 8.3:+9545692 | None:intergenic |
| CAAATGATCATCGAACAAGC+TGG | 0.507973 | 8.3:-9540953 | MS.gene58777:CDS |
| TCTGCCAAGGATGCGGGTGA+TGG | 0.508021 | 8.3:+9540995 | None:intergenic |
| ACCAGGTACTGCTGCAACCA+TGG | 0.510558 | 8.3:+9539962 | None:intergenic |
| ATCCCTAGCAATAACAGCAC+TGG | 0.511203 | 8.3:-9547462 | MS.gene58777:CDS |
| ACCATGGTTGCAGCAGTACC+TGG | 0.511482 | 8.3:-9539963 | MS.gene58777:CDS |
| CAAGACTTCTGCCAAGGATG+CGG | 0.512149 | 8.3:+9540988 | None:intergenic |
| GTATTTCTCCAGTGGGAAAG+AGG | 0.513409 | 8.3:+9539118 | None:intergenic |
| TACTGGTCCTAATCATATCA+TGG | 0.514762 | 8.3:-9540443 | MS.gene58777:CDS |
| AATATCAATATTCTGGCAGC+TGG | 0.514856 | 8.3:-9539207 | MS.gene58777:CDS |
| TTAAGAACCCTCTTTCCCAC+TGG | 0.515894 | 8.3:-9539126 | MS.gene58777:CDS |
| TCCAGATCCAAAACGCCAAA+AGG | 0.518937 | 8.3:-9538444 | MS.gene58777:CDS |
| GGCTCATAGTAATGAAGCAA+TGG | 0.522818 | 8.3:-9541059 | MS.gene58777:CDS |
| TCAAGTTTAGGTAGTGGACT+TGG | 0.524401 | 8.3:-9540278 | MS.gene58777:CDS |
| AGATGGTCACACATTGCATC+TGG | 0.525681 | 8.3:-9544920 | MS.gene58777:CDS |
| TTCCAGTGCTGTTATTGCTA+GGG | 0.526167 | 8.3:+9547460 | None:intergenic |
| AGAGTGTATCAGATAGATTA+CGG | 0.527320 | 8.3:-9541450 | MS.gene58777:CDS |
| TATACCCTATTTATTTCAGG+TGG | 0.531188 | 8.3:+9542668 | None:intergenic |
| GCGATCAACCACTCAATCTA+GGG | 0.536771 | 8.3:-9540684 | MS.gene58777:CDS |
| CTGCTAGTCAAGCAACCTCT+AGG | 0.538894 | 8.3:-9540040 | MS.gene58777:CDS |
| AAATGATCATCGAACAAGCT+GGG | 0.539445 | 8.3:-9540952 | MS.gene58777:CDS |
| CCACTCAATCTAGGGCATTG+AGG | 0.539741 | 8.3:-9540676 | MS.gene58777:CDS |
| GCTCATAGTAATGAAGCAAT+GGG | 0.540566 | 8.3:-9541058 | MS.gene58777:CDS |
| CGTGCTTTGCCTTTGCAATG+TGG | 0.541533 | 8.3:+9539779 | None:intergenic |
| GTGGAGGATTCAAGCTTACA+AGG | 0.541912 | 8.3:-9539093 | MS.gene58777:CDS |
| ATGCCAGCCAAGAAGAGCGT+GGG | 0.546687 | 8.3:-9540106 | MS.gene58777:CDS |
| GTGAACCAGTTTGATCAAGT+AGG | 0.547727 | 8.3:+9541473 | None:intergenic |
| AGATTGAGTGGTTGATCGCA+AGG | 0.548030 | 8.3:+9540688 | None:intergenic |
| TGGTGAAGGGATTGTTGTCA+GGG | 0.549615 | 8.3:-9542514 | MS.gene58777:intron |
| CAGCAGCGCTTAATTTGCCA+AGG | 0.554164 | 8.3:-9545709 | MS.gene58777:CDS |
| TCTCCAGCAATAGATGCATC+CGG | 0.554311 | 8.3:+9540014 | None:intergenic |
| GCTCCAGCTTGAAAAGGAAG+AGG | 0.556671 | 8.3:+9540341 | None:intergenic |
| TCAATATTCTGGCAGCTGGT+GGG | 0.561013 | 8.3:-9539203 | MS.gene58777:CDS |
| GGGGAATGCCCAAGCAGCTG+AGG | 0.561312 | 8.3:-9541080 | MS.gene58777:CDS |
| GGAAGTCTGCCCAATCATGC+AGG | 0.562465 | 8.3:-9544868 | MS.gene58777:intron |
| AATGCATGCTGCAACATCAA+GGG | 0.563664 | 8.3:-9539049 | MS.gene58777:CDS |
| TGATCAAGTAGGGGACGGAA+TGG | 0.565104 | 8.3:+9541484 | None:intergenic |
| CAATCTGTTCTTTCAAAGCA+GGG | 0.571681 | 8.3:+9545758 | None:intergenic |
| ACTGACTCAAGTTTAGGTAG+TGG | 0.575634 | 8.3:-9540284 | MS.gene58777:CDS |
| TCTTGTGTTTGGAGTTGATG+AGG | 0.577749 | 8.3:+9539257 | None:intergenic |
| TTCTGAAGAAGCACGACCCA+AGG | 0.578001 | 8.3:+9539938 | None:intergenic |
| ACGGAAGACTCTGCAAGGAA+TGG | 0.578919 | 8.3:-9539759 | MS.gene58777:intron |
| ATGATATGATTAGGACCAGT+AGG | 0.579197 | 8.3:+9540445 | None:intergenic |
| AACAGCAGGTTCAGCTTCAG+GGG | 0.580718 | 8.3:+9539014 | None:intergenic |
| TATTTCTCCAGTGGGAAAGA+GGG | 0.581137 | 8.3:+9539119 | None:intergenic |
| AGCTTTGGTGCTGTCCCTGT+AGG | 0.581153 | 8.3:-9540317 | MS.gene58777:CDS |
| AACCATCTGGTAATTCTACT+GGG | 0.583839 | 8.3:-9539917 | MS.gene58777:CDS |
| CCTATTTGTTGAGATATGAC+CGG | 0.584034 | 8.3:+9538946 | None:intergenic |
| CTCCACATGTATTTCTCCAG+TGG | 0.584365 | 8.3:+9539110 | None:intergenic |
| ATTCACGACTCATATGACTT+AGG | 0.585176 | 8.3:+9541221 | None:intergenic |
| TCAACGTGTAACTACAGGGC+AGG | 0.585805 | 8.3:-9539868 | MS.gene58777:CDS |
| CACATTGCAAAGGCAAAGCA+CGG | 0.586185 | 8.3:-9539778 | MS.gene58777:CDS |
| CAGTCTGTGCAGCTCCTACA+GGG | 0.586356 | 8.3:+9540303 | None:intergenic |
| ACAAACTTATACTCTCAAAG+TGG | 0.586665 | 8.3:-9547394 | MS.gene58777:CDS |
| CACTGGAGAAATACATGTGG+AGG | 0.586666 | 8.3:-9539109 | MS.gene58777:CDS |
| GATTGAGTGGTTGATCGCAA+GGG | 0.587750 | 8.3:+9540689 | None:intergenic |
| GAAGCACGACCCAAGGGACC+AGG | 0.587781 | 8.3:+9539945 | None:intergenic |
| TTGGGCAGACTTCCTGGAGG+TGG | 0.588696 | 8.3:+9544877 | None:intergenic |
| GAACCTGCTGTTAGTGCTGA+AGG | 0.590701 | 8.3:-9539003 | MS.gene58777:CDS |
| GACAAAGTTGTCAAAGAACC+AGG | 0.591819 | 8.3:+9541250 | None:intergenic |
| GGGACATCGCGCAGACATAG+TGG | 0.592620 | 8.3:-9538482 | MS.gene58777:CDS |
| TCCACATGTATTTCTCCAGT+GGG | 0.593901 | 8.3:+9539111 | None:intergenic |
| ATATGACCGGCATGATCTCA+CGG | 0.595492 | 8.3:+9538959 | None:intergenic |
| AATGTTTCAATGAATACACC+TGG | 0.600607 | 8.3:+9542719 | None:intergenic |
| ACACAAGTAGATGTTGAAGA+TGG | 0.601424 | 8.3:-9544937 | MS.gene58777:intron |
| TGGAATCCGAAGTTCTTCCA+AGG | 0.602911 | 8.3:+9541521 | None:intergenic |
| ACTCCTTCAGCACTAACAGC+AGG | 0.604034 | 8.3:+9539000 | None:intergenic |
| CAACCTGATCTACCACCTCC+AGG | 0.605336 | 8.3:-9544889 | MS.gene58777:CDS |
| TTACTTGCAGTCAGAGAAGG+GGG | 0.606399 | 8.3:-9541100 | MS.gene58777:intron |
| CCTCAATGCCCTAGATTGAG+TGG | 0.609007 | 8.3:+9540676 | None:intergenic |
| GGAAGAACTTCGGATTCCAG+TGG | 0.611373 | 8.3:-9541517 | MS.gene58777:CDS |
| TTTACTTGCAGTCAGAGAAG+GGG | 0.611476 | 8.3:-9541101 | MS.gene58777:intron |
| TGTAGGTTGAAACTAATGCT+GGG | 0.614804 | 8.3:-9538502 | MS.gene58777:intron |
| ATTGCGTTTGGGTCAAACAC+CGG | 0.617351 | 8.3:-9540591 | MS.gene58777:intron |
| ACACATTGCATCTGGTTGTG+AGG | 0.620498 | 8.3:-9544912 | MS.gene58777:CDS |
| TCTGAAGCTGTAGTTAATGG+TGG | 0.620579 | 8.3:-9540487 | MS.gene58777:CDS |
| GATAGTGCCGATTAGAACCA+TGG | 0.621547 | 8.3:-9539979 | MS.gene58777:CDS |
| AATGCCAGCCAAGAAGAGCG+TGG | 0.622070 | 8.3:-9540107 | MS.gene58777:CDS |
| ATTGAGTGGTTGATCGCAAG+GGG | 0.622548 | 8.3:+9540690 | None:intergenic |
| ACGTTGAAATCTCCCAAGAA+CGG | 0.622703 | 8.3:+9539884 | None:intergenic |
| CAGTTTGATCAAGTAGGGGA+CGG | 0.624652 | 8.3:+9541479 | None:intergenic |
| AGATGCATCCGGACGCCTAG+AGG | 0.624898 | 8.3:+9540025 | None:intergenic |
| CTCCTGAATCCACATTGCAA+AGG | 0.625624 | 8.3:-9539788 | MS.gene58777:CDS |
| ATGAAGGAGCATGATTCCCA+AGG | 0.626007 | 8.3:-9541544 | MS.gene58777:intron |
| AAAGCACGGAAGACTCTGCA+AGG | 0.628187 | 8.3:-9539764 | MS.gene58777:CDS |
| TTGACCCAAACGCAATGTCA+TGG | 0.628505 | 8.3:+9540598 | None:intergenic |
| TGTACTCCGTGAGATCATGC+CGG | 0.632422 | 8.3:-9538965 | MS.gene58777:CDS |
| GGCTCAAGATTCATCAACCC+AGG | 0.632427 | 8.3:-9538893 | MS.gene58777:intron |
| AAATTCTTCTGATGATCATG+CGG | 0.635928 | 8.3:-9538914 | MS.gene58777:CDS |
| GATTTCAACGTGTAACTACA+GGG | 0.640232 | 8.3:-9539872 | MS.gene58777:CDS |
| TGATGTTGCAGCATGCATTG+AGG | 0.640694 | 8.3:+9539053 | None:intergenic |
| ATGCATGCTGCAACATCAAG+GGG | 0.641170 | 8.3:-9539048 | MS.gene58777:CDS |
| AATGATCATCGAACAAGCTG+GGG | 0.648848 | 8.3:-9540951 | MS.gene58777:CDS |
| AGAAAGCAAGACTTCTGCCA+AGG | 0.651398 | 8.3:+9540982 | None:intergenic |
| TCTGAAGAAGCACGACCCAA+GGG | 0.651864 | 8.3:+9539939 | None:intergenic |
| GGGCAGGCAAATAGTGAACA+GGG | 0.653751 | 8.3:-9539852 | MS.gene58777:CDS |
| ACTCATATGACTTAGGTACT+GGG | 0.654035 | 8.3:+9541228 | None:intergenic |
| AGCAGCGCTTAATTTGCCAA+GGG | 0.655567 | 8.3:-9545708 | MS.gene58777:CDS |
| TGCCAGCCAAGAAGAGCGTG+GGG | 0.658703 | 8.3:-9540105 | MS.gene58777:CDS |
| TGTTGCAGCATGCATTGAGG+TGG | 0.659497 | 8.3:+9539056 | None:intergenic |
| GAAATCTCCCAAGAACGGGA+TGG | 0.659546 | 8.3:+9539889 | None:intergenic |
| TTCCGGCGTCAGTTTCTCTG+GGG | 0.663260 | 8.3:-9541414 | MS.gene58777:CDS |
| AGCCCAGTAGAATTACCAGA+TGG | 0.672721 | 8.3:+9539915 | None:intergenic |
| GACCCCAGAGAAACTGACGC+CGG | 0.688330 | 8.3:+9541412 | None:intergenic |
| TACTTGCAGTCAGAGAAGGG+GGG | 0.692681 | 8.3:-9541099 | MS.gene58777:intron |
| TCCCACTGGAGAAATACATG+TGG | 0.695949 | 8.3:-9539112 | MS.gene58777:CDS |
| GTGGATTCAGGAGCTGAATG+TGG | 0.704038 | 8.3:+9539798 | None:intergenic |
| GAACCAGTTTGATCAAGTAG+GGG | 0.707025 | 8.3:+9541475 | None:intergenic |
| ATCGATATACAGATAAGACG+AGG | 0.726970 | 8.3:-9540233 | MS.gene58777:intron |
| TTGAGTGGTTGATCGCAAGG+GGG | 0.754069 | 8.3:+9540691 | None:intergenic |
| TGATTGGGCAGACTTCCTGG+AGG | 0.764411 | 8.3:+9544874 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 9538424 | 9547508 | 9538424 | ID=MS.gene58777 |
| chr8.3 | mRNA | 9538424 | 9547508 | 9538424 | ID=MS.gene58777.t1;Parent=MS.gene58777 |
| chr8.3 | exon | 9547386 | 9547508 | 9547386 | ID=MS.gene58777.t1.exon1;Parent=MS.gene58777.t1 |
| chr8.3 | CDS | 9547386 | 9547508 | 9547386 | ID=cds.MS.gene58777.t1;Parent=MS.gene58777.t1 |
| chr8.3 | exon | 9545671 | 9545788 | 9545671 | ID=MS.gene58777.t1.exon2;Parent=MS.gene58777.t1 |
| chr8.3 | CDS | 9545671 | 9545788 | 9545671 | ID=cds.MS.gene58777.t1;Parent=MS.gene58777.t1 |
| chr8.3 | exon | 9544869 | 9544949 | 9544869 | ID=MS.gene58777.t1.exon3;Parent=MS.gene58777.t1 |
| chr8.3 | CDS | 9544869 | 9544949 | 9544869 | ID=cds.MS.gene58777.t1;Parent=MS.gene58777.t1 |
| chr8.3 | exon | 9542673 | 9542791 | 9542673 | ID=MS.gene58777.t1.exon4;Parent=MS.gene58777.t1 |
| chr8.3 | CDS | 9542673 | 9542791 | 9542673 | ID=cds.MS.gene58777.t1;Parent=MS.gene58777.t1 |
| chr8.3 | exon | 9542515 | 9542589 | 9542515 | ID=MS.gene58777.t1.exon5;Parent=MS.gene58777.t1 |
| chr8.3 | CDS | 9542515 | 9542589 | 9542515 | ID=cds.MS.gene58777.t1;Parent=MS.gene58777.t1 |
| chr8.3 | exon | 9541399 | 9541560 | 9541399 | ID=MS.gene58777.t1.exon6;Parent=MS.gene58777.t1 |
| chr8.3 | CDS | 9541399 | 9541560 | 9541399 | ID=cds.MS.gene58777.t1;Parent=MS.gene58777.t1 |
| chr8.3 | exon | 9541209 | 9541278 | 9541209 | ID=MS.gene58777.t1.exon7;Parent=MS.gene58777.t1 |
| chr8.3 | CDS | 9541209 | 9541278 | 9541209 | ID=cds.MS.gene58777.t1;Parent=MS.gene58777.t1 |
| chr8.3 | exon | 9540940 | 9541112 | 9540940 | ID=MS.gene58777.t1.exon8;Parent=MS.gene58777.t1 |
| chr8.3 | CDS | 9540940 | 9541112 | 9540940 | ID=cds.MS.gene58777.t1;Parent=MS.gene58777.t1 |
| chr8.3 | exon | 9540592 | 9540756 | 9540592 | ID=MS.gene58777.t1.exon9;Parent=MS.gene58777.t1 |
| chr8.3 | CDS | 9540592 | 9540756 | 9540592 | ID=cds.MS.gene58777.t1;Parent=MS.gene58777.t1 |
| chr8.3 | exon | 9540438 | 9540509 | 9540438 | ID=MS.gene58777.t1.exon10;Parent=MS.gene58777.t1 |
| chr8.3 | CDS | 9540438 | 9540509 | 9540438 | ID=cds.MS.gene58777.t1;Parent=MS.gene58777.t1 |
| chr8.3 | exon | 9540234 | 9540363 | 9540234 | ID=MS.gene58777.t1.exon11;Parent=MS.gene58777.t1 |
| chr8.3 | CDS | 9540234 | 9540363 | 9540234 | ID=cds.MS.gene58777.t1;Parent=MS.gene58777.t1 |
| chr8.3 | exon | 9539760 | 9540146 | 9539760 | ID=MS.gene58777.t1.exon12;Parent=MS.gene58777.t1 |
| chr8.3 | CDS | 9539760 | 9540146 | 9539760 | ID=cds.MS.gene58777.t1;Parent=MS.gene58777.t1 |
| chr8.3 | exon | 9538894 | 9539282 | 9538894 | ID=MS.gene58777.t1.exon13;Parent=MS.gene58777.t1 |
| chr8.3 | CDS | 9538894 | 9539282 | 9538894 | ID=cds.MS.gene58777.t1;Parent=MS.gene58777.t1 |
| chr8.3 | exon | 9538424 | 9538519 | 9538424 | ID=MS.gene58777.t1.exon14;Parent=MS.gene58777.t1 |
| chr8.3 | CDS | 9538424 | 9538519 | 9538424 | ID=cds.MS.gene58777.t1;Parent=MS.gene58777.t1 |
| Gene Sequence |
| Protein sequence |
