Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58778.t1 | XP_003609498.2 | 96 | 150 | 6 | 0 | 1 | 150 | 1 | 150 | 1.30E-69 | 272.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58778.t1 | G7JGN5 | 96.0 | 150 | 6 | 0 | 1 | 150 | 1 | 150 | 9.5e-70 | 272.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053104 | MS.gene58778 | 0.804555 | 2.04E-49 | -1.69E-46 |
| MS.gene059960 | MS.gene58778 | 0.80594 | 1.05E-49 | -1.69E-46 |
| MS.gene060804 | MS.gene58778 | 0.805124 | 1.55E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58778.t1 | MTR_4g116360 | 96.000 | 150 | 6 | 0 | 1 | 150 | 1 | 150 | 3.92e-95 | 271 |
| MS.gene58778.t1 | MTR_2g450420 | 46.809 | 141 | 63 | 5 | 18 | 150 | 17 | 153 | 1.01e-29 | 105 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 25 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGGCTCACACCTCAGTAAA+TGG | 0.325930 | 8.3:-9554092 | MS.gene58778:CDS |
| TAGACTCAATTAATTGTGTA+TGG | 0.388334 | 8.3:+9553935 | None:intergenic |
| CTGCAGGAAATATAAAGAAT+AGG | 0.441652 | 8.3:-9554282 | MS.gene58778:intron |
| CTAGAAATGTGAATGTGAAT+GGG | 0.472633 | 8.3:-9554064 | MS.gene58778:CDS |
| GTGTGTGCAGGGATACGTTT+GGG | 0.472885 | 8.3:-9555002 | MS.gene58778:intron |
| GTTATGTCTACAAGGTCCTC+AGG | 0.481183 | 8.3:+9553993 | None:intergenic |
| ACTAGAAATGTGAATGTGAA+TGG | 0.484726 | 8.3:-9554065 | MS.gene58778:CDS |
| GCAGGGATACGTTTGGGAAA+AGG | 0.491954 | 8.3:-9554996 | MS.gene58778:intron |
| TGTGTGTGCAGGGATACGTT+TGG | 0.497727 | 8.3:-9555003 | MS.gene58778:intron |
| TACAACTAAATCCTTGTCAA+AGG | 0.499214 | 8.3:-9554236 | MS.gene58778:intron |
| CCATCTCAGTTATGTCTACA+AGG | 0.517334 | 8.3:+9553985 | None:intergenic |
| CCTTGTAGACATAACTGAGA+TGG | 0.558484 | 8.3:-9553985 | MS.gene58778:CDS |
| TTTAGTTGTAGAAATTGAAG+TGG | 0.566395 | 8.3:+9554249 | None:intergenic |
| TGTGAAGAACACTGAGGATG+TGG | 0.567568 | 8.3:-9554111 | MS.gene58778:CDS |
| AGAGAGACTGCTACACAAGA+AGG | 0.574354 | 8.3:+9555134 | None:intergenic |
| AGACTCAATTAATTGTGTAT+GGG | 0.578163 | 8.3:+9553936 | None:intergenic |
| AAGAAGTAACACTGATTCTG+AGG | 0.585024 | 8.3:-9554809 | MS.gene58778:CDS |
| AGATGGATTATTCACCAGCT+AGG | 0.588549 | 8.3:-9553968 | MS.gene58778:CDS |
| TCTAGTATTGCCATTTACTG+AGG | 0.596383 | 8.3:+9554082 | None:intergenic |
| TTATAAACCAACCTTTGACA+AGG | 0.606206 | 8.3:+9554225 | None:intergenic |
| TTATGTCTACAAGGTCCTCA+GGG | 0.619907 | 8.3:+9553994 | None:intergenic |
| TAGAAATGTGAATGTGAATG+GGG | 0.652177 | 8.3:-9554063 | MS.gene58778:CDS |
| GAATGTGAATGGGGAAGCAA+AGG | 0.660105 | 8.3:-9554054 | MS.gene58778:CDS |
| TACTTCTAATTATCTCCCTG+AGG | 0.676211 | 8.3:-9554009 | MS.gene58778:CDS |
| ACAGAATGTGAAGAACACTG+AGG | 0.882326 | 8.3:-9554117 | MS.gene58778:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATAAAGATGTAAAAAAAA+AGG | + | chr8.3:9554922-9554941 | None:intergenic | 10.0% |
| !! | AATAAAGATGTAAAAAAAAA+GGG | + | chr8.3:9554921-9554940 | None:intergenic | 10.0% |
| !! | ATAAATTATATTATAAACCT+TGG | - | chr8.3:9554890-9554909 | MS.gene58778:intron | 10.0% |
| !! | ATCATGAAAGAAATTTAATT+AGG | - | chr8.3:9554709-9554728 | MS.gene58778:intron | 15.0% |
| !! | TAATTAAATTTCTTTCATGA+TGG | + | chr8.3:9554710-9554729 | None:intergenic | 15.0% |
| !! | TCAAATCTACAATTGTAAAA+TGG | + | chr8.3:9554535-9554554 | None:intergenic | 20.0% |
| !! | TGGAAAATTATTATCTAAAG+TGG | - | chr8.3:9554500-9554519 | MS.gene58778:intron | 20.0% |
| !!! | TTTTAAAAAAATTAGGGTAG+TGG | - | chr8.3:9554480-9554499 | MS.gene58778:intron | 20.0% |
| ! | AAAAAAAAAGGGAAATTCCA+AGG | + | chr8.3:9554910-9554929 | None:intergenic | 25.0% |
| ! | AAAAAAGGAAGAAAAACTTG+TGG | + | chr8.3:9554458-9554477 | None:intergenic | 25.0% |
| ! | ATATGTCCTTGTAATTTCAA+AGG | + | chr8.3:9554565-9554584 | None:intergenic | 25.0% |
| ! | TTTAGTTGTAGAAATTGAAG+TGG | + | chr8.3:9554838-9554857 | None:intergenic | 25.0% |
| !! | ATTTACCTTGAGTTTTTGTA+AGG | + | chr8.3:9553994-9554013 | None:intergenic | 25.0% |
| !! | TTGCATAGTCATAAGTTTTT+GGG | + | chr8.3:9554615-9554634 | None:intergenic | 25.0% |
| !! | TTTGCATAGTCATAAGTTTT+TGG | + | chr8.3:9554616-9554635 | None:intergenic | 25.0% |
| !!! | AAATGTTTTTATTTTGCTGC+AGG | - | chr8.3:9554786-9554805 | MS.gene58778:CDS | 25.0% |
| ACTAGAAATGTGAATGTGAA+TGG | - | chr8.3:9555019-9555038 | MS.gene58778:intron | 30.0% | |
| CAGATTCATGAAGATTACTA+TGG | - | chr8.3:9554390-9554409 | MS.gene58778:intron | 30.0% | |
| CTAGAAATGTGAATGTGAAT+GGG | - | chr8.3:9555020-9555039 | MS.gene58778:intron | 30.0% | |
| CTGCAGGAAATATAAAGAAT+AGG | - | chr8.3:9554802-9554821 | MS.gene58778:CDS | 30.0% | |
| GGAAGATTAGAAATGTAACA+AGG | + | chr8.3:9554056-9554075 | None:intergenic | 30.0% | |
| TACAACTAAATCCTTGTCAA+AGG | - | chr8.3:9554848-9554867 | MS.gene58778:intron | 30.0% | |
| TAGAAATGTGAATGTGAATG+GGG | - | chr8.3:9555021-9555040 | MS.gene58778:intron | 30.0% | |
| TAGTAATCTTCATGAATCTG+TGG | + | chr8.3:9554390-9554409 | None:intergenic | 30.0% | |
| TATGACTATGCAAAAATGCA+AGG | - | chr8.3:9554622-9554641 | MS.gene58778:intron | 30.0% | |
| TGTGGACTTGAAAAAACTAA+TGG | + | chr8.3:9554440-9554459 | None:intergenic | 30.0% | |
| TTATAAACCAACCTTTGACA+AGG | + | chr8.3:9554862-9554881 | None:intergenic | 30.0% | |
| ! | TGCATGCTTTCACATTTTTA+TGG | - | chr8.3:9554192-9554211 | MS.gene58778:intron | 30.0% |
| !!! | AGTGTTACTTCTTTTCAACA+AGG | + | chr8.3:9554268-9554287 | None:intergenic | 30.0% |
| AATGACCAACAAAAATGCAC+AGG | - | chr8.3:9554312-9554331 | MS.gene58778:intron | 35.0% | |
| ACTAAATCCTTGTCAAAGGT+TGG | - | chr8.3:9554852-9554871 | MS.gene58778:intron | 35.0% | |
| CTTCTCCTTACAAAAACTCA+AGG | - | chr8.3:9553986-9554005 | MS.gene58778:CDS | 35.0% | |
| GACAAAATTTGTCAGCTTAG+TGG | + | chr8.3:9554593-9554612 | None:intergenic | 35.0% | |
| GAGCAACAAAAACAACATGT+AGG | - | chr8.3:9554124-9554143 | MS.gene58778:intron | 35.0% | |
| TACTTCTAATTATCTCCCTG+AGG | - | chr8.3:9555075-9555094 | MS.gene58778:intron | 35.0% | |
| TATTCTCTTCTCTTGGTTTC+TGG | - | chr8.3:9554648-9554667 | MS.gene58778:intron | 35.0% | |
| ! | ATTATCTAAAGTGGCGCTAA+TGG | - | chr8.3:9554509-9554528 | MS.gene58778:intron | 35.0% |
| ! | TAACTGGTTGTGTGATTTTC+TGG | + | chr8.3:9554367-9554386 | None:intergenic | 35.0% |
| ! | TCTAGTATTGCCATTTACTG+AGG | + | chr8.3:9555005-9555024 | None:intergenic | 35.0% |
| !! | AAGAAGTAACACTGATTCTG+AGG | - | chr8.3:9554275-9554294 | MS.gene58778:CDS | 35.0% |
| !! | AAGGCTTTATTCTCTTCTCT+TGG | - | chr8.3:9554641-9554660 | MS.gene58778:intron | 35.0% |
| !! | CTTCGTCCTTTGAAATTACA+AGG | - | chr8.3:9554556-9554575 | MS.gene58778:intron | 35.0% |
| !! | CTTTAGAAGCTGTTTTCTTC+AGG | + | chr8.3:9554341-9554360 | None:intergenic | 35.0% |
| ACAGAATGTGAAGAACACTG+AGG | - | chr8.3:9554967-9554986 | MS.gene58778:CDS | 40.0% | |
| CCATCTCAGTTATGTCTACA+AGG | + | chr8.3:9555102-9555121 | None:intergenic | 40.0% | |
| CCTTGTAGACATAACTGAGA+TGG | - | chr8.3:9555099-9555118 | MS.gene58778:CDS | 40.0% | |
| CTTCATGAATCTGTGGTAAC+TGG | + | chr8.3:9554383-9554402 | None:intergenic | 40.0% | |
| TATCCCTGCACACACAAAAA+AGG | + | chr8.3:9554077-9554096 | None:intergenic | 40.0% | |
| TTATGTCTACAAGGTCCTCA+GGG | + | chr8.3:9555093-9555112 | None:intergenic | 40.0% | |
| !! | AAGTTCACTTTTCTGACTGC+AGG | - | chr8.3:9554227-9554246 | MS.gene58778:intron | 40.0% |
| !! | AGATGGATTATTCACCAGCT+AGG | - | chr8.3:9555116-9555135 | MS.gene58778:CDS | 40.0% |
| !!! | CTTCCTTTTTTGTGTGTGCA+GGG | - | chr8.3:9554071-9554090 | MS.gene58778:CDS | 40.0% |
| !!! | TCTTCCTTTTTTGTGTGTGC+AGG | - | chr8.3:9554070-9554089 | MS.gene58778:CDS | 40.0% |
| AGAGAGACTGCTACACAAGA+AGG | + | chr8.3:9553953-9553972 | None:intergenic | 45.0% | |
| GAATGTGAATGGGGAAGCAA+AGG | - | chr8.3:9555030-9555049 | MS.gene58778:intron | 45.0% | |
| GACCTAGACGACATAGATCT+AGG | - | chr8.3:9554412-9554431 | MS.gene58778:intron | 45.0% | |
| GTTATGTCTACAAGGTCCTC+AGG | + | chr8.3:9555094-9555113 | None:intergenic | 45.0% | |
| TGCCTAGATCTATGTCGTCT+AGG | + | chr8.3:9554417-9554436 | None:intergenic | 45.0% | |
| TGTGAAGAACACTGAGGATG+TGG | - | chr8.3:9554973-9554992 | MS.gene58778:CDS | 45.0% | |
| ! | ACTTTTCTGACTGCAGGATG+AGG | - | chr8.3:9554233-9554252 | MS.gene58778:intron | 45.0% |
| ! | GGCTACCTGTGCATTTTTGT+TGG | + | chr8.3:9554320-9554339 | None:intergenic | 45.0% |
| !! | ATGGGAGACTTTTTCCTAGC+TGG | + | chr8.3:9555133-9555152 | None:intergenic | 45.0% |
| !!! | AATTTTTTTAAAAGAAAAAA+AGG | + | chr8.3:9554473-9554492 | None:intergenic | 5.0% |
| !!! | TTTTTCTTTTAAAAAAATTA+GGG | - | chr8.3:9554474-9554493 | MS.gene58778:intron | 5.0% |
| !!! | TTTTTTCTTTTAAAAAAATT+AGG | - | chr8.3:9554473-9554492 | MS.gene58778:intron | 5.0% |
| GCAGGGATACGTTTGGGAAA+AGG | - | chr8.3:9554088-9554107 | MS.gene58778:CDS | 50.0% | |
| GTGGCTCACACCTCAGTAAA+TGG | - | chr8.3:9554992-9555011 | MS.gene58778:CDS | 50.0% | |
| GTGTGTGCAGGGATACGTTT+GGG | - | chr8.3:9554082-9554101 | MS.gene58778:CDS | 50.0% | |
| TGTGTGTGCAGGGATACGTT+TGG | - | chr8.3:9554081-9554100 | MS.gene58778:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 9553944 | 9555162 | 9553944 | ID=MS.gene58778 |
| chr8.3 | mRNA | 9553944 | 9555162 | 9553944 | ID=MS.gene58778.t1;Parent=MS.gene58778 |
| chr8.3 | exon | 9555099 | 9555162 | 9555099 | ID=MS.gene58778.t1.exon1;Parent=MS.gene58778.t1 |
| chr8.3 | CDS | 9555099 | 9555162 | 9555099 | ID=cds.MS.gene58778.t1;Parent=MS.gene58778.t1 |
| chr8.3 | exon | 9554965 | 9555014 | 9554965 | ID=MS.gene58778.t1.exon2;Parent=MS.gene58778.t1 |
| chr8.3 | CDS | 9554965 | 9555014 | 9554965 | ID=cds.MS.gene58778.t1;Parent=MS.gene58778.t1 |
| chr8.3 | exon | 9554773 | 9554857 | 9554773 | ID=MS.gene58778.t1.exon3;Parent=MS.gene58778.t1 |
| chr8.3 | CDS | 9554773 | 9554857 | 9554773 | ID=cds.MS.gene58778.t1;Parent=MS.gene58778.t1 |
| chr8.3 | exon | 9554237 | 9554298 | 9554237 | ID=MS.gene58778.t1.exon4;Parent=MS.gene58778.t1 |
| chr8.3 | CDS | 9554237 | 9554298 | 9554237 | ID=cds.MS.gene58778.t1;Parent=MS.gene58778.t1 |
| chr8.3 | exon | 9553944 | 9554135 | 9553944 | ID=MS.gene58778.t1.exon5;Parent=MS.gene58778.t1 |
| chr8.3 | CDS | 9553944 | 9554135 | 9553944 | ID=cds.MS.gene58778.t1;Parent=MS.gene58778.t1 |
| Gene Sequence |
| Protein sequence |
