Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59254.t1 | RHN38533.1 | 86.8 | 91 | 12 | 0 | 5 | 95 | 20 | 110 | 7.90E-36 | 159.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59254.t1 | G7ZUE5 | 86.8 | 91 | 12 | 0 | 5 | 95 | 243 | 333 | 5.7e-36 | 159.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052447 | MS.gene59254 | 0.800611 | 1.33E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59254.t1 | MTR_4g023110 | 74.725 | 91 | 22 | 1 | 5 | 95 | 9 | 98 | 2.22e-45 | 143 |
| MS.gene59254.t1 | MTR_3g058800 | 71.579 | 95 | 26 | 1 | 1 | 95 | 6 | 99 | 8.95e-45 | 142 |
| MS.gene59254.t1 | MTR_2g020090 | 71.277 | 94 | 26 | 1 | 2 | 95 | 61 | 153 | 4.25e-44 | 142 |
| MS.gene59254.t1 | MTR_0029s0140 | 86.813 | 91 | 12 | 0 | 5 | 95 | 243 | 333 | 6.21e-44 | 146 |
| MS.gene59254.t1 | MTR_2g102860 | 87.059 | 85 | 11 | 0 | 11 | 95 | 13 | 97 | 1.07e-41 | 134 |
| MS.gene59254.t1 | MTR_4g017750 | 81.720 | 93 | 17 | 0 | 3 | 95 | 109 | 201 | 1.12e-41 | 137 |
| MS.gene59254.t1 | MTR_1g077650 | 67.778 | 90 | 28 | 1 | 5 | 94 | 47 | 135 | 3.88e-41 | 134 |
| MS.gene59254.t1 | MTR_5g080310 | 79.570 | 93 | 19 | 0 | 3 | 95 | 120 | 212 | 4.40e-41 | 136 |
| MS.gene59254.t1 | MTR_1g023240 | 83.721 | 86 | 14 | 0 | 10 | 95 | 35 | 120 | 1.11e-37 | 127 |
| MS.gene59254.t1 | MTR_6g463490 | 90.476 | 63 | 6 | 0 | 33 | 95 | 3 | 65 | 5.49e-37 | 121 |
| MS.gene59254.t1 | MTR_8g027455 | 65.556 | 90 | 30 | 1 | 5 | 94 | 25 | 113 | 1.13e-36 | 128 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 28 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATGATTGAGGCTGAGTCTA+AGG | 0.271938 | 6.4:+45070226 | None:intergenic |
| CTGGCATCAGCTCAGGCTGC+TGG | 0.304850 | 6.4:+45070190 | None:intergenic |
| CGCACTTGGACAAAAGTTGT+TGG | 0.380454 | 6.4:-45070324 | MS.gene59254:CDS |
| ATTCTTTGTCCTTCCGCACT+TGG | 0.388853 | 6.4:-45070338 | MS.gene59254:CDS |
| AAGAAGAAAGGGAAGCTATA+TGG | 0.394700 | 6.4:-45070297 | MS.gene59254:CDS |
| TGGCATCAGCTCAGGCTGCT+GGG | 0.409514 | 6.4:+45070191 | None:intergenic |
| TGAATAAGCTCCTGCATCTC+TGG | 0.428977 | 6.4:+45070171 | None:intergenic |
| TGCATCTCTGGCATCAGCTC+AGG | 0.441852 | 6.4:+45070183 | None:intergenic |
| TGAGCTGATGCCAGAGATGC+AGG | 0.443993 | 6.4:-45070181 | MS.gene59254:CDS |
| AAAGGGAAGCTATATGGTCT+TGG | 0.453421 | 6.4:-45070291 | MS.gene59254:CDS |
| AACACTACCCTTCCGATAGT+TGG | 0.493187 | 6.4:+45070257 | None:intergenic |
| GTTGTTGGCGACAAGAAGAA+AGG | 0.505708 | 6.4:-45070309 | MS.gene59254:CDS |
| TTTGCCGAGGCTATTTCTCA+AGG | 0.512005 | 6.4:-45070375 | MS.gene59254:CDS |
| GAAACTTAGCCGCCAACTAT+CGG | 0.535401 | 6.4:-45070269 | MS.gene59254:CDS |
| CTTAGACTCAGCCTCAATCA+TGG | 0.540588 | 6.4:-45070225 | MS.gene59254:CDS |
| AGGATCCTTCACCATGATTG+AGG | 0.557439 | 6.4:+45070214 | None:intergenic |
| CTCAGCCTCAATCATGGTGA+AGG | 0.569664 | 6.4:-45070219 | MS.gene59254:CDS |
| TTGTTGGCGACAAGAAGAAA+GGG | 0.576833 | 6.4:-45070308 | MS.gene59254:CDS |
| AAATAGCCTCGGCAAACTGC+CGG | 0.585843 | 6.4:+45070382 | None:intergenic |
| TTGAACAGGAGGAGTATGAC+CGG | 0.586858 | 6.4:-45070401 | MS.gene59254:intron |
| ATGATTGAGGCTGAGTCTAA+GGG | 0.596575 | 6.4:+45070227 | None:intergenic |
| CATCAGCTCAGGCTGCTGGG+AGG | 0.597386 | 6.4:+45070194 | None:intergenic |
| TTGCCGAGGCTATTTCTCAA+GGG | 0.597756 | 6.4:-45070374 | MS.gene59254:CDS |
| ACTACCCTTCCGATAGTTGG+CGG | 0.600620 | 6.4:+45070260 | None:intergenic |
| CTTAGCCGCCAACTATCGGA+AGG | 0.616038 | 6.4:-45070265 | MS.gene59254:CDS |
| GTATGACCGGCAGTTTGCCG+AGG | 0.658653 | 6.4:-45070388 | MS.gene59254:CDS |
| TGACCCTTGAGAAATAGCCT+CGG | 0.667208 | 6.4:+45070371 | None:intergenic |
| TTAGCCGCCAACTATCGGAA+GGG | 0.671704 | 6.4:-45070264 | MS.gene59254:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATAATAACAAAATAGTTA+AGG | + | chr6.4:45070530-45070549 | None:intergenic | 10.0% |
| !! | AAAAAATTAGGTGATTAATA+AGG | - | chr6.4:45070226-45070245 | MS.gene59254:CDS | 15.0% |
| !!! | TATTTTGTTATTATTTGAAC+AGG | - | chr6.4:45070534-45070553 | MS.gene59254:intron | 15.0% |
| !! | CAGATTTGATATATGATTAA+TGG | - | chr6.4:45070288-45070307 | MS.gene59254:CDS | 20.0% |
| !! | TACTAGTTATTGTTTAATGT+TGG | - | chr6.4:45070438-45070457 | MS.gene59254:intron | 20.0% |
| ! | AGAAAATTCAAGACAAGAAA+AGG | + | chr6.4:45070330-45070349 | None:intergenic | 25.0% |
| ! | GAAAATTCAAGACAAGAAAA+GGG | + | chr6.4:45070329-45070348 | None:intergenic | 25.0% |
| ! | TAGACTATAAAGCATAAACT+AGG | - | chr6.4:45070466-45070485 | MS.gene59254:intron | 25.0% |
| !!! | AATCACCTAATTTTTTGTTC+AGG | + | chr6.4:45070222-45070241 | None:intergenic | 25.0% |
| !!! | TTTGTTATTATTTGAACAGG+AGG | - | chr6.4:45070537-45070556 | MS.gene59254:intron | 25.0% |
| CAATATAAAGCAGAACAAAG+AGG | + | chr6.4:45070405-45070424 | None:intergenic | 30.0% | |
| TGCAAAAAATGAAAGATAGC+TGG | - | chr6.4:45070265-45070284 | MS.gene59254:CDS | 30.0% | |
| TGCAACCTGAACAAAAAATT+AGG | - | chr6.4:45070214-45070233 | MS.gene59254:CDS | 30.0% | |
| AAAATAGTTAAGGACACCAC+TGG | + | chr6.4:45070520-45070539 | None:intergenic | 35.0% | |
| AAGAAGAAAGGGAAGCTATA+TGG | - | chr6.4:45070652-45070671 | MS.gene59254:intron | 35.0% | |
| AGCTATACTAGAAAATCCAC+AGG | + | chr6.4:45070192-45070211 | None:intergenic | 35.0% | |
| ! | CTTGAATTTTCTTCTGAGCA+GGG | - | chr6.4:45070338-45070357 | MS.gene59254:CDS | 35.0% |
| ! | TCTTGAATTTTCTTCTGAGC+AGG | - | chr6.4:45070337-45070356 | MS.gene59254:CDS | 35.0% |
| ! | TTTACATCTGTTTTGTCCTG+TGG | - | chr6.4:45070173-45070192 | MS.gene59254:CDS | 35.0% |
| AAAGGGAAGCTATATGGTCT+TGG | - | chr6.4:45070658-45070677 | MS.gene59254:intron | 40.0% | |
| ATACACAGAATACTCTCCAG+TGG | - | chr6.4:45070501-45070520 | MS.gene59254:intron | 40.0% | |
| ATGATTGAGGCTGAGTCTAA+GGG | + | chr6.4:45070725-45070744 | None:intergenic | 40.0% | |
| CAGAACAAAGAGGTCTGTAA+TGG | + | chr6.4:45070395-45070414 | None:intergenic | 40.0% | |
| TTGTTGGCGACAAGAAGAAA+GGG | - | chr6.4:45070641-45070660 | MS.gene59254:intron | 40.0% | |
| ! | CAACAACTTTTGTCCAAGTG+CGG | + | chr6.4:45070627-45070646 | None:intergenic | 40.0% |
| AACACTACCCTTCCGATAGT+TGG | + | chr6.4:45070695-45070714 | None:intergenic | 45.0% | |
| AGGATCCTTCACCATGATTG+AGG | + | chr6.4:45070738-45070757 | None:intergenic | 45.0% | |
| ATTCTTTGTCCTTCCGCACT+TGG | - | chr6.4:45070611-45070630 | MS.gene59254:intron | 45.0% | |
| CATGATTGAGGCTGAGTCTA+AGG | + | chr6.4:45070726-45070745 | None:intergenic | 45.0% | |
| CGCACTTGGACAAAAGTTGT+TGG | - | chr6.4:45070625-45070644 | MS.gene59254:intron | 45.0% | |
| CTTAGACTCAGCCTCAATCA+TGG | - | chr6.4:45070724-45070743 | MS.gene59254:intron | 45.0% | |
| GAAACTTAGCCGCCAACTAT+CGG | - | chr6.4:45070680-45070699 | MS.gene59254:intron | 45.0% | |
| GTTGTTGGCGACAAGAAGAA+AGG | - | chr6.4:45070640-45070659 | MS.gene59254:intron | 45.0% | |
| TGAATAAGCTCCTGCATCTC+TGG | + | chr6.4:45070781-45070800 | None:intergenic | 45.0% | |
| TGACCCTTGAGAAATAGCCT+CGG | + | chr6.4:45070581-45070600 | None:intergenic | 45.0% | |
| ! | AACTTTTGTCCAAGTGCGGA+AGG | + | chr6.4:45070623-45070642 | None:intergenic | 45.0% |
| ! | TTGAACAGGAGGAGTATGAC+CGG | - | chr6.4:45070548-45070567 | MS.gene59254:intron | 45.0% |
| !! | TTGCCGAGGCTATTTCTCAA+GGG | - | chr6.4:45070575-45070594 | MS.gene59254:intron | 45.0% |
| !! | TTTGCCGAGGCTATTTCTCA+AGG | - | chr6.4:45070574-45070593 | MS.gene59254:intron | 45.0% |
| AAATAGCCTCGGCAAACTGC+CGG | + | chr6.4:45070570-45070589 | None:intergenic | 50.0% | |
| ACTACCCTTCCGATAGTTGG+CGG | + | chr6.4:45070692-45070711 | None:intergenic | 50.0% | |
| CTCAGCCTCAATCATGGTGA+AGG | - | chr6.4:45070730-45070749 | MS.gene59254:intron | 50.0% | |
| CTTCTGAGCAGGGTTTGCAT+TGG | - | chr6.4:45070348-45070367 | MS.gene59254:CDS | 50.0% | |
| TTAGCCGCCAACTATCGGAA+GGG | - | chr6.4:45070685-45070704 | MS.gene59254:intron | 50.0% | |
| CTTAGCCGCCAACTATCGGA+AGG | - | chr6.4:45070684-45070703 | MS.gene59254:intron | 55.0% | |
| TGAGCTGATGCCAGAGATGC+AGG | - | chr6.4:45070768-45070787 | MS.gene59254:intron | 55.0% | |
| TGCATCTCTGGCATCAGCTC+AGG | + | chr6.4:45070769-45070788 | None:intergenic | 55.0% | |
| GTATGACCGGCAGTTTGCCG+AGG | - | chr6.4:45070561-45070580 | MS.gene59254:intron | 60.0% | |
| TGGCATCAGCTCAGGCTGCT+GGG | + | chr6.4:45070761-45070780 | None:intergenic | 60.0% | |
| CATCAGCTCAGGCTGCTGGG+AGG | + | chr6.4:45070758-45070777 | None:intergenic | 65.0% | |
| CTGGCATCAGCTCAGGCTGC+TGG | + | chr6.4:45070762-45070781 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 45070140 | 45070831 | 45070140 | ID=MS.gene59254 |
| chr6.4 | mRNA | 45070140 | 45070831 | 45070140 | ID=MS.gene59254.t1;Parent=MS.gene59254 |
| chr6.4 | exon | 45070820 | 45070831 | 45070820 | ID=MS.gene59254.t1.exon1;Parent=MS.gene59254.t1 |
| chr6.4 | CDS | 45070820 | 45070831 | 45070820 | ID=cds.MS.gene59254.t1;Parent=MS.gene59254.t1 |
| chr6.4 | exon | 45070140 | 45070415 | 45070140 | ID=MS.gene59254.t1.exon2;Parent=MS.gene59254.t1 |
| chr6.4 | CDS | 45070140 | 45070415 | 45070140 | ID=cds.MS.gene59254.t1;Parent=MS.gene59254.t1 |
| Gene Sequence |
| Protein sequence |
