AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene59383

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59383.t1 MTR_5g020570 94.263 767 42 1 1 765 1 767 0.0 1452
MS.gene59383.t1 MTR_5g020570 94.263 767 42 1 1 765 16 782 0.0 1451
MS.gene59383.t1 MTR_8g077205 56.069 758 318 7 1 753 1 748 0.0 829
MS.gene59383.t1 MTR_8g077205 55.728 646 271 7 113 753 1 636 0.0 693
MS.gene59383.t1 MTR_8g080280 30.864 729 433 15 19 711 19 712 1.13e-98 322
MS.gene59383.t1 MTR_3g085610 29.649 769 486 15 10 751 9 749 5.19e-96 315
MS.gene59383.t1 MTR_1g022350 40.952 210 121 2 19 226 16 224 7.50e-37 138
MS.gene59383.t1 MTR_1g069845 28.195 493 287 16 271 711 122 599 9.14e-32 132
MS.gene59383.t1 MTR_1g069845 28.195 493 287 16 271 711 239 716 3.28e-31 130
MS.gene59383.t1 MTR_1g069845 28.195 493 287 16 271 711 243 720 3.74e-31 130
MS.gene59383.t1 MTR_7g116600 38.571 140 84 1 225 362 28 167 2.30e-27 116
MS.gene59383.t1 MTR_7g053260 24.835 455 244 16 242 660 54 446 3.03e-24 107
MS.gene59383.t1 MTR_4g485520 48.235 85 44 0 264 348 96 180 1.53e-23 105
MS.gene59383.t1 MTR_4g485520 48.235 85 44 0 264 348 264 348 3.01e-23 105
MS.gene59383.t1 MTR_4g063800 26.250 320 186 11 41 348 49 330 1.01e-21 100
MS.gene59383.t1 MTR_2g018010 20.721 444 281 10 274 708 293 674 3.03e-21 99.4
MS.gene59383.t1 MTR_3g466220 47.778 90 46 1 266 354 225 314 5.77e-21 99.0
MS.gene59383.t1 MTR_3g466220 47.778 90 46 1 266 354 204 293 6.80e-21 98.6
MS.gene59383.t1 MTR_1g079450 39.175 97 59 0 274 370 64 160 1.30e-20 95.9
MS.gene59383.t1 MTR_7g078330 39.286 112 59 1 265 367 266 377 2.80e-20 96.3
MS.gene59383.t1 MTR_7g059405 48.837 86 41 1 274 359 265 347 2.08e-19 93.6
MS.gene59383.t1 MTR_7g097020 48.611 72 37 0 277 348 245 316 1.22e-18 91.3
MS.gene59383.t1 MTR_7g097020 30.208 288 161 12 451 711 484 758 7.24e-12 69.3
MS.gene59383.t1 MTR_1g093965 39.844 128 70 3 286 409 1 125 1.69e-18 85.1
MS.gene59383.t1 MTR_5g083030 47.297 74 39 0 274 347 294 367 5.43e-18 89.0
MS.gene59383.t1 MTR_5g034440 45.122 82 45 0 275 356 282 363 8.02e-18 88.6
MS.gene59383.t1 MTR_1g094215 53.333 75 35 0 274 348 65 139 1.63e-17 86.3
MS.gene59383.t1 MTR_1g094215 53.333 75 35 0 274 348 248 322 1.79e-17 87.4
MS.gene59383.t1 MTR_4g028960 27.178 287 160 7 63 341 103 348 2.48e-17 87.0
MS.gene59383.t1 MTR_1g093995 39.062 128 71 3 286 409 1 125 5.29e-17 80.5
MS.gene59383.t1 MTR_4g107010 40.964 83 49 0 274 356 281 363 2.55e-16 84.0
MS.gene59383.t1 MTR_3g078160 42.500 80 46 0 274 353 276 355 1.05e-15 81.6
MS.gene59383.t1 MTR_3g078160 42.500 80 46 0 274 353 276 355 1.09e-15 81.6
MS.gene59383.t1 MTR_3g078160 42.500 80 46 0 274 353 276 355 2.38e-15 80.1
MS.gene59383.t1 MTR_4g085720 40.000 100 55 3 274 369 15 113 3.22e-15 79.0
MS.gene59383.t1 MTR_1g056840 44.330 97 40 6 265 350 1 94 8.75e-14 74.7
MS.gene59383.t1 MTR_5g048050 38.095 84 48 2 271 350 23 106 1.27e-13 74.3
MS.gene59383.t1 MTR_6g071340 38.095 84 48 2 271 350 3 86 1.55e-13 73.9
MS.gene59383.t1 MTR_3g065080 37.209 86 50 2 269 350 32 117 1.76e-13 73.9
MS.gene59383.t1 MTR_5g077510 39.286 84 47 2 271 350 33 116 1.88e-13 73.6
MS.gene59383.t1 MTR_1g056870 41.509 106 48 6 265 359 1 103 2.05e-13 73.6
MS.gene59383.t1 MTR_6g004790 31.280 211 130 7 522 721 86 292 2.08e-13 72.4
MS.gene59383.t1 MTR_1g056880 41.509 106 48 6 265 359 1 103 2.60e-13 73.2
MS.gene59383.t1 MTR_4g091880 47.761 67 34 1 274 339 6 72 8.63e-13 70.9
MS.gene59383.t1 MTR_4g091880 47.761 67 34 1 274 339 6 72 1.02e-12 70.9
MS.gene59383.t1 MTR_8g103227 36.275 102 61 2 251 348 370 471 1.78e-12 71.6
MS.gene59383.t1 MTR_5g069340 29.545 176 115 3 541 710 143 315 2.76e-12 69.3
MS.gene59383.t1 MTR_2g026130 28.743 167 112 5 548 711 290 452 6.87e-12 68.9
MS.gene59383.t1 MTR_1g056910 42.045 88 45 4 269 350 2 89 9.03e-12 68.2
MS.gene59383.t1 MTR_5g079550 24.528 212 114 3 544 710 89 299 1.50e-11 67.4
MS.gene59383.t1 MTR_3g096370 36.842 76 47 1 265 339 1 76 1.85e-11 67.4
MS.gene59383.t1 MTR_3g095730 39.130 69 42 0 271 339 11 79 3.15e-11 66.6
MS.gene59383.t1 MTR_7g106340 41.573 89 45 4 269 350 4 92 3.38e-11 66.6
MS.gene59383.t1 MTR_2g011140 44.615 65 36 0 275 339 17 81 6.96e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59383.t1 AT4G36550 36.111 756 403 17 17 753 12 706 1.26e-135 418
MS.gene59383.t1 AT1G24330 32.873 724 423 16 19 711 17 708 6.99e-109 350
MS.gene59383.t1 AT1G24330 32.873 724 423 16 19 711 17 708 6.99e-109 350
MS.gene59383.t1 AT1G67530 32.577 749 455 20 19 746 17 736 2.26e-106 343
MS.gene59383.t1 AT1G67530 32.577 749 455 20 19 746 17 736 2.26e-106 343
MS.gene59383.t1 AT1G67530 32.577 749 455 20 19 746 17 736 2.26e-106 343
MS.gene59383.t1 AT1G27910 30.992 726 432 16 19 711 17 706 2.83e-88 295
MS.gene59383.t1 AT1G67530 35.282 496 291 12 19 502 17 494 3.77e-84 278
MS.gene59383.t1 AT5G67340 25.322 466 308 11 274 708 241 697 6.33e-35 142
MS.gene59383.t1 AT5G67340 25.322 466 308 11 274 708 241 697 6.33e-35 142
MS.gene59383.t1 AT3G07360 53.333 75 35 0 271 345 72 146 6.23e-25 109
MS.gene59383.t1 AT3G46510 29.341 334 199 12 23 350 31 333 6.92e-25 110
MS.gene59383.t1 AT5G42340 27.327 333 189 10 27 349 77 366 4.53e-24 108
MS.gene59383.t1 AT2G28830 48.276 87 45 0 262 348 173 259 1.85e-22 102
MS.gene59383.t1 AT2G23140 45.455 88 48 0 267 354 227 314 9.25e-22 101
MS.gene59383.t1 AT2G23140 28.090 178 118 4 535 711 654 822 1.78e-12 71.6
MS.gene59383.t1 AT2G23140 45.455 88 48 0 267 354 224 311 9.71e-22 101
MS.gene59383.t1 AT2G23140 28.090 178 118 4 535 711 651 819 1.90e-12 71.6
MS.gene59383.t1 AT2G23140 45.455 88 48 0 267 354 224 311 9.71e-22 101
MS.gene59383.t1 AT2G23140 28.090 178 118 4 535 711 651 819 1.90e-12 71.6
MS.gene59383.t1 AT2G23140 45.455 88 48 0 267 354 35 122 1.05e-21 100
MS.gene59383.t1 AT2G23140 28.090 178 118 4 535 711 462 630 1.15e-12 72.0
MS.gene59383.t1 AT2G23140 45.455 88 48 0 267 354 35 122 1.05e-21 100
MS.gene59383.t1 AT2G23140 28.090 178 118 4 535 711 462 630 1.15e-12 72.0
MS.gene59383.t1 AT3G54850 52.000 75 36 0 274 348 249 323 1.45e-21 100
MS.gene59383.t1 AT1G23030 43.333 90 51 0 259 348 227 316 2.07e-21 99.8
MS.gene59383.t1 AT1G71020 42.000 100 54 1 249 348 75 170 2.75e-20 95.5
MS.gene59383.t1 AT1G71020 42.000 100 54 1 249 348 223 318 5.99e-20 95.1
MS.gene59383.t1 AT3G54790 39.535 86 52 0 271 356 200 285 3.70e-18 89.7
MS.gene59383.t1 AT3G54790 39.535 86 52 0 271 356 236 321 5.07e-18 89.4
MS.gene59383.t1 AT3G54790 39.535 86 52 0 271 356 236 321 5.07e-18 89.4
MS.gene59383.t1 AT5G18340 33.108 148 86 3 223 358 12 158 5.65e-16 81.6
MS.gene59383.t1 AT5G01830 37.634 93 49 1 274 357 275 367 9.58e-16 82.0
MS.gene59383.t1 AT1G29340 44.737 76 42 0 271 346 303 378 1.11e-15 81.6
MS.gene59383.t1 AT5G18320 44.444 81 44 1 271 351 70 149 3.54e-15 79.3
MS.gene59383.t1 AT1G49780 45.679 81 40 2 274 350 15 95 4.03e-15 79.0
MS.gene59383.t1 AT1G66160 39.130 92 52 2 263 350 23 114 7.14e-14 75.1
MS.gene59383.t1 AT3G19380 43.902 82 41 3 274 350 15 96 8.17e-14 74.7
MS.gene59383.t1 AT1G10560 35.849 106 67 1 248 352 262 367 1.55e-13 74.7
MS.gene59383.t1 AT5G37490 40.909 88 47 2 267 350 26 112 2.80e-13 73.2
MS.gene59383.t1 AT2G35930 45.122 82 40 3 274 350 13 94 9.08e-13 71.6
MS.gene59383.t1 AT3G52450 43.902 82 41 3 274 350 8 89 1.41e-12 70.9
MS.gene59383.t1 AT1G01680 40.244 82 49 0 262 343 222 303 2.24e-12 69.3
MS.gene59383.t1 AT5G18330 25.321 312 180 12 271 577 63 326 2.94e-12 70.1
MS.gene59383.t1 AT3G61390 36.842 76 48 0 264 339 269 344 8.26e-12 68.2
MS.gene59383.t1 AT1G60190 39.024 82 50 0 275 356 280 361 8.33e-12 69.3
MS.gene59383.t1 AT3G61390 36.842 76 48 0 264 339 344 419 1.53e-11 67.8
MS.gene59383.t1 AT3G49810 46.970 66 35 0 274 339 65 130 2.20e-11 67.4
MS.gene59383.t1 AT1G56040 36.667 90 52 1 249 338 334 418 2.88e-11 66.6

Find 120 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCAAATACCCTCTCCTTTC+AGG 0.129941 5.4:+12520829 MS.gene59383:CDS
TGCATTGGATGCTGCAATTT+TGG 0.187482 5.4:-12523187 None:intergenic
GGATTATCTTAAGTTGATTT+TGG 0.208826 5.4:+12523551 MS.gene59383:CDS
AAGTCCTTCATGACAATATT+TGG 0.222378 5.4:-12522271 None:intergenic
ATAACTTCCCAAACTCTTAA+TGG 0.232924 5.4:-12522386 None:intergenic
GCCATAATGGAAGAACTTTC+TGG 0.238374 5.4:+12523147 MS.gene59383:CDS
TCAAAGTCGTCCACGGAATC+TGG 0.239441 5.4:-12521746 None:intergenic
GTAGAACTGTTGTTGGTAAT+TGG 0.247713 5.4:-12523705 None:intergenic
ACAAAGCTTAATTCCCATTC+AGG 0.250995 5.4:+12521448 MS.gene59383:CDS
AATCACCTCCATTAAGAGTT+TGG 0.258899 5.4:+12522378 MS.gene59383:CDS
TCCAGAAAGTTCTTCCATTA+TGG 0.272030 5.4:-12523148 None:intergenic
AGATCGTTTCTCGATTAAAA+TGG 0.277574 5.4:-12521822 None:intergenic
GATCCTGTTATCATTGCTTC+TGG 0.293374 5.4:+12522151 MS.gene59383:CDS
ACCTGAAAGGAGAGGGTATT+TGG 0.293706 5.4:-12520830 None:intergenic
TACATCAACCTTGAGGATAT+TGG 0.317123 5.4:-12522127 None:intergenic
TGAGTGTGTTACATTTGTTC+TGG 0.318243 5.4:+12521664 MS.gene59383:CDS
CAACATGTCACTTGGATCAT+TGG 0.319176 5.4:+12522441 MS.gene59383:CDS
CAGCATCATGAGAATATTTC+CGG 0.326397 5.4:+12521109 MS.gene59383:CDS
ATCACCTCCATTAAGAGTTT+GGG 0.353633 5.4:+12522379 MS.gene59383:CDS
CTCAACTTCATGATCTTCAA+TGG 0.371229 5.4:+12522605 MS.gene59383:CDS
CTTCTTGATTCAGAAGTGAT+AGG 0.387240 5.4:+12523114 MS.gene59383:CDS
AATCCTAGTTTCTTCAGAAA+TGG 0.387275 5.4:-12523774 None:intergenic
ACTTATGCATCCCTTTGTTT+CGG 0.390748 5.4:-12523436 None:intergenic
AGGAGAAGTACTTGCCATAA+TGG 0.393561 5.4:+12523134 MS.gene59383:CDS
TTCCGACAGAAGCTGAGTTC+CGG 0.397671 5.4:-12522779 None:intergenic
GACTGGAACCATACCCTGAA+TGG 0.398503 5.4:-12521462 None:intergenic
CTTAATTCCCATTCAGGGTA+TGG 0.404422 5.4:+12521454 MS.gene59383:CDS
TCACAGAATGGAAATGATAA+AGG 0.407952 5.4:+12523606 MS.gene59383:CDS
TTATTCTTATAGCATGTTGT+TGG 0.411310 5.4:-12523254 None:intergenic
CTAGAGTTATCCCTTGCAAT+TGG 0.428602 5.4:-12521989 None:intergenic
ACTCCAGAAGCAATGATAAC+AGG 0.429341 5.4:-12522154 None:intergenic
TGAGATGTTATATTCAGTCT+TGG 0.439810 5.4:-12521794 None:intergenic
TCCAGTCATGTTGGCTGTAG+AGG 0.442914 5.4:+12521478 MS.gene59383:CDS
CAAAGTCGTCCACGGAATCT+GGG 0.444410 5.4:-12521745 None:intergenic
AAATCTTCTGTATGTCTACT+TGG 0.449559 5.4:-12522337 None:intergenic
ACCTCTACAGCCAACATGAC+TGG 0.454239 5.4:-12521479 None:intergenic
GGCCGTGCTTCCTCTATGTC+CGG 0.455061 5.4:-12521128 None:intergenic
AAGAAGTTTCTAGATAAAGT+TGG 0.465531 5.4:+12521848 MS.gene59383:CDS
ATTACTTCTGCAACATTGCT+CGG 0.466898 5.4:+12521211 MS.gene59383:CDS
TCTGTATGTCTACTTGGATC+AGG 0.467341 5.4:-12522331 None:intergenic
TCAACTTCATGATCTTCAAT+GGG 0.469345 5.4:+12522606 MS.gene59383:CDS
CATAGAGTTGATGCAAATAG+TGG 0.470306 5.4:+12521085 MS.gene59383:CDS
CATCAGATTCAAGTCATGAT+TGG 0.470843 5.4:-12522500 None:intergenic
ATTGGACACTTGTATTCCTC+AGG 0.479427 5.4:-12522109 None:intergenic
ACTGAATATAACATCTCAAA+AGG 0.479795 5.4:+12521799 MS.gene59383:CDS
GCACGGCCAAGATGTTCATC+AGG 0.480753 5.4:+12521143 MS.gene59383:CDS
TATTACTTTCAGGCTGTGAC+AGG 0.485539 5.4:+12521380 MS.gene59383:intron
TCACCATTTCTGAAGAAACT+AGG 0.488630 5.4:+12523771 MS.gene59383:CDS
GATTCTGCTGAAGAAGAGGC+TGG 0.489454 5.4:+12521686 MS.gene59383:CDS
GACGTAAAAGCGCTGAGAGC+CGG 0.495304 5.4:+12522760 MS.gene59383:CDS
AATTCTATTGCCGAAACAAA+GGG 0.496586 5.4:+12523426 MS.gene59383:CDS
TACTTGATTCTGATAACAAA+GGG 0.497678 5.4:+12523229 MS.gene59383:CDS
CTCTTAATGAGAACCAATAG+TGG 0.499914 5.4:+12520788 None:intergenic
GGAATCTGTAGAACTGTTGT+TGG 0.501183 5.4:-12523712 None:intergenic
ATGGAAGAACTTTCTGGAGA+TGG 0.503922 5.4:+12523153 MS.gene59383:CDS
TTATGAACGTATCTTCACTT+AGG 0.504024 5.4:-12523080 None:intergenic
GAGCAATGCAAATGATCAGC+AGG 0.504932 5.4:+12522738 MS.gene59383:CDS
TTTATTTATGTGTACCTGAA+AGG 0.505342 5.4:-12520843 None:intergenic
ACTGGAACCATACCCTGAAT+GGG 0.507557 5.4:-12521461 None:intergenic
GTAAGAGAATTGCTTCATCA+AGG 0.507831 5.4:+12521716 MS.gene59383:CDS
ATGTACATGTTCTCAAAATG+TGG 0.513586 5.4:+12523530 MS.gene59383:CDS
CAAAGCTTAATTCCCATTCA+GGG 0.514357 5.4:+12521449 MS.gene59383:CDS
GACTCCAAATATTGTCATGA+AGG 0.514758 5.4:+12522267 MS.gene59383:CDS
TCTATTCCTGATGAACATCT+TGG 0.521143 5.4:-12521149 None:intergenic
GCATGGAAACTTAATGTTGG+TGG 0.522527 5.4:+12521928 MS.gene59383:CDS
GGAATGCAGCAAACTCTACC+TGG 0.530347 5.4:+12521232 MS.gene59383:CDS
TTTGGAGTCAATGTCATATG+AGG 0.534604 5.4:-12522253 None:intergenic
AACATTTGTGATACTGAAGA+AGG 0.537856 5.4:+12523399 MS.gene59383:CDS
TGGACAAACTTCACTGTTGT+AGG 0.541725 5.4:-12523289 None:intergenic
GACATGTTGGTAAGATCCAT+TGG 0.542509 5.4:-12522427 None:intergenic
GCATGAACTGTATGCTTGTG+AGG 0.545176 5.4:-12522550 None:intergenic
AAAGAATGTGGATAAGAAAG+TGG 0.545796 5.4:+12522185 MS.gene59383:CDS
CAATGATCTGAACTTGCCAA+TGG 0.550541 5.4:+12522411 MS.gene59383:CDS
TTACTTGATTCTGATAACAA+AGG 0.553043 5.4:+12523228 MS.gene59383:CDS
CATGGAAACTTAATGTTGGT+GGG 0.555579 5.4:+12521929 MS.gene59383:CDS
GATTTCTTCGTCGAGAATCA+CGG 0.555802 5.4:-12522012 None:intergenic
GAGAATATTTCCGGACATAG+AGG 0.559119 5.4:+12521118 MS.gene59383:CDS
GATCTTACCAACATGTCACT+TGG 0.560709 5.4:+12522433 MS.gene59383:CDS
TTCTGTGAGATATAGAAAAG+AGG 0.564373 5.4:-12523591 None:intergenic
GGGTATGGTTCCAGTCATGT+TGG 0.567915 5.4:+12521469 MS.gene59383:CDS
ATGTCTACTTGGATCAGGAA+TGG 0.568043 5.4:-12522326 None:intergenic
AAATTCTATTGCCGAAACAA+AGG 0.571416 5.4:+12523425 MS.gene59383:CDS
CAATGATCCAAGTGACATGT+TGG 0.572790 5.4:-12522440 None:intergenic
TCTGGATTCTGCTGAAGAAG+AGG 0.573607 5.4:+12521682 MS.gene59383:CDS
TAAGACAGAAGTGAGTGCAT+TGG 0.577623 5.4:-12523202 None:intergenic
GATAAGAAAGTGGATGAATG+AGG 0.579915 5.4:+12522195 MS.gene59383:CDS
AGCCGGAACTCAGCTTCTGT+CGG 0.579942 5.4:+12522777 MS.gene59383:CDS
ATTGCTGAAATACTTGAGAG+TGG 0.582850 5.4:+12523462 MS.gene59383:CDS
GGACACTTGTATTCCTCAGG+TGG 0.587812 5.4:-12522106 None:intergenic
TTCCGGACATAGAGGAAGCA+CGG 0.596773 5.4:+12521126 MS.gene59383:CDS
AGGTTCAACCCCAGATTCCG+TGG 0.599696 5.4:+12521736 MS.gene59383:CDS
CTGACACTGACAAAGTAGCT+TGG 0.600806 5.4:-12522681 None:intergenic
ATTCTGCTGAAGAAGAGGCT+GGG 0.601067 5.4:+12521687 MS.gene59383:CDS
GAGTAACATATGAAAGAATG+TGG 0.601967 5.4:+12522173 MS.gene59383:CDS
TATGTGTACCTGAAAGGAGA+GGG 0.606017 5.4:-12520837 None:intergenic
CAGTGAAGTTTGTCCACACA+TGG 0.607997 5.4:+12523296 MS.gene59383:CDS
AATGTTGGTGGGAGAACACA+TGG 0.609389 5.4:+12521940 MS.gene59383:CDS
GAATGGAAATGATAAAGGAA+AGG 0.610121 5.4:+12523611 MS.gene59383:CDS
GATGAATCAGTCGGATAGAG+TGG 0.610590 5.4:+12522081 MS.gene59383:CDS
AATGTGTTGAGAATGAAGAT+AGG 0.612607 5.4:+12523673 MS.gene59383:CDS
TCTAACTTCAAAGTCGTCCA+CGG 0.613649 5.4:-12521753 None:intergenic
CAAAATATGAAGCAATTCCA+CGG 0.616213 5.4:-12523640 None:intergenic
ATAAGAAAGTGGATGAATGA+GGG 0.616816 5.4:+12522196 MS.gene59383:CDS
TAGAGAAGAACCAATTGCAA+GGG 0.618427 5.4:+12521979 MS.gene59383:CDS
CATTGAAGATCATGAAGTTG+AGG 0.619411 5.4:-12522604 None:intergenic
TAAAACTGAGATGAATCAGT+CGG 0.620886 5.4:+12522072 MS.gene59383:CDS
GGTCTTAACTTGTGACACAT+CGG 0.622908 5.4:-12522479 None:intergenic
AAAGCATGGAAACTTAATGT+TGG 0.624675 5.4:+12521925 MS.gene59383:CDS
GTAGAGAAGAACCAATTGCA+AGG 0.624828 5.4:+12521978 MS.gene59383:CDS
TTATGTGTACCTGAAAGGAG+AGG 0.628672 5.4:-12520838 None:intergenic
ACAAGTGTCCAATATCCTCA+AGG 0.631386 5.4:+12522119 MS.gene59383:CDS
AAAGTCGTCCACGGAATCTG+GGG 0.632373 5.4:-12521744 None:intergenic
GGATAGAGTGGCACCACCTG+AGG 0.636060 5.4:+12522093 MS.gene59383:CDS
ACTTCCCAAACTCTTAATGG+AGG 0.650645 5.4:-12522383 None:intergenic
TAAAGGAAAGGAAAGTGCCG+TGG 0.657866 5.4:+12523623 MS.gene59383:CDS
CACATGCATCAAAACCCAGA+AGG 0.661406 5.4:+12523814 MS.gene59383:CDS
TGAAGGACTTGATATCAAAG+TGG 0.687601 5.4:+12522284 MS.gene59383:CDS
AGGATCATACATCAACCTTG+AGG 0.687795 5.4:-12522134 None:intergenic
CAATTGACAGATACCATGTG+TGG 0.700444 5.4:-12523309 None:intergenic
GTTGTTGGTAATTGGCTGAG+AGG 0.715093 5.4:-12523697 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAAATATAAAACTCAATT+TGG - chr5.4:12521614-12521633 None:intergenic 15.0%
!! TATAAATAATTAAGTTCCTA+AGG + chr5.4:12522843-12522862 MS.gene59383:intron 15.0%
!!! ATTTCTTTATGTTTTTAGAA+AGG - chr5.4:12521273-12521292 None:intergenic 15.0%
!! AAATTTATAATACCAAAAGC+AGG - chr5.4:12523015-12523034 None:intergenic 20.0%
!! TATATGTCCAAAAACAAAAA+AGG + chr5.4:12522225-12522244 MS.gene59383:CDS 20.0%
!!! AGTTTTATATTTTCTGTTGT+AGG + chr5.4:12521619-12521638 MS.gene59383:intron 20.0%
!!! TATTTTGTTCATCATATCAT+AGG + chr5.4:12520882-12520901 MS.gene59383:intron 20.0%
!!! TGAAAAAAGGTAACAATTTT+GGG - chr5.4:12523338-12523357 None:intergenic 20.0%
!!! TTGAAAAAAGGTAACAATTT+TGG - chr5.4:12523339-12523358 None:intergenic 20.0%
! AACAAAATAGTGAAATGCAA+TGG - chr5.4:12520871-12520890 None:intergenic 25.0%
! ACTGAATATAACATCTCAAA+AGG + chr5.4:12521799-12521818 MS.gene59383:CDS 25.0%
! ATGTATCTAATGAAAAAGCA+TGG + chr5.4:12521911-12521930 MS.gene59383:CDS 25.0%
! CGGAATTTATGTTAATTAAC+AGG + chr5.4:12521046-12521065 MS.gene59383:intron 25.0%
! GAATTTGTGAAATTGTAGAA+AGG + chr5.4:12522799-12522818 MS.gene59383:intron 25.0%
! TACTTGATTCTGATAACAAA+GGG + chr5.4:12523229-12523248 MS.gene59383:CDS 25.0%
! TTACTTGATTCTGATAACAA+AGG + chr5.4:12523228-12523247 MS.gene59383:CDS 25.0%
! TTATTCTTATAGCATGTTGT+TGG - chr5.4:12523257-12523276 None:intergenic 25.0%
! TTGGTTTCAATATTACTTTC+AGG + chr5.4:12521370-12521389 MS.gene59383:intron 25.0%
! TTTATTTATGTGTACCTGAA+AGG - chr5.4:12520846-12520865 None:intergenic 25.0%
!! AAGAAGTTTCTAGATAAAGT+TGG + chr5.4:12521848-12521867 MS.gene59383:CDS 25.0%
!! ATATCGTTTGTTAGTTAGAT+TGG - chr5.4:12520941-12520960 None:intergenic 25.0%
!! TACAGAAGATTTTATGTTGT+TGG + chr5.4:12522348-12522367 MS.gene59383:CDS 25.0%
!!! GGATTATCTTAAGTTGATTT+TGG + chr5.4:12523551-12523570 MS.gene59383:CDS 25.0%
!!! TTTGATGATCTATTTTCTTC+AGG - chr5.4:12523747-12523766 None:intergenic 25.0%
AAAGAATGTGGATAAGAAAG+TGG + chr5.4:12522185-12522204 MS.gene59383:CDS 30.0%
AAAGCATGGAAACTTAATGT+TGG + chr5.4:12521925-12521944 MS.gene59383:CDS 30.0%
AAATCTTCTGTATGTCTACT+TGG - chr5.4:12522340-12522359 None:intergenic 30.0%
AAATTCTATTGCCGAAACAA+AGG + chr5.4:12523425-12523444 MS.gene59383:CDS 30.0%
AACATTTGTGATACTGAAGA+AGG + chr5.4:12523399-12523418 MS.gene59383:CDS 30.0%
AAGTCCTTCATGACAATATT+TGG - chr5.4:12522274-12522293 None:intergenic 30.0%
AATCCTAGTTTCTTCAGAAA+TGG - chr5.4:12523777-12523796 None:intergenic 30.0%
AATGTGTTGAGAATGAAGAT+AGG + chr5.4:12523673-12523692 MS.gene59383:CDS 30.0%
AATTCTATTGCCGAAACAAA+GGG + chr5.4:12523426-12523445 MS.gene59383:CDS 30.0%
AGATCGTTTCTCGATTAAAA+TGG - chr5.4:12521825-12521844 None:intergenic 30.0%
AGGAAAAAAAAATACACACC+AGG - chr5.4:12521253-12521272 None:intergenic 30.0%
ATAACTTCCCAAACTCTTAA+TGG - chr5.4:12522389-12522408 None:intergenic 30.0%
ATAAGAAAGTGGATGAATGA+GGG + chr5.4:12522196-12522215 MS.gene59383:CDS 30.0%
ATGTACATGTTCTCAAAATG+TGG + chr5.4:12523530-12523549 MS.gene59383:CDS 30.0%
CAAAATATGAAGCAATTCCA+CGG - chr5.4:12523643-12523662 None:intergenic 30.0%
CAAGAAACTTAAAATTTGCG+TGG - chr5.4:12522951-12522970 None:intergenic 30.0%
GAATGGAAATGATAAAGGAA+AGG + chr5.4:12523611-12523630 MS.gene59383:CDS 30.0%
GAGTAACATATGAAAGAATG+TGG + chr5.4:12522173-12522192 MS.gene59383:CDS 30.0%
TAAAACTGAGATGAATCAGT+CGG + chr5.4:12522072-12522091 MS.gene59383:CDS 30.0%
TCAAAGTGGTGCAAAAATAA+TGG + chr5.4:12522298-12522317 MS.gene59383:CDS 30.0%
TCAACTTCATGATCTTCAAT+GGG + chr5.4:12522606-12522625 MS.gene59383:CDS 30.0%
TCAATCATATTAGTCCATGT+CGG - chr5.4:12522873-12522892 None:intergenic 30.0%
TCACAGAATGGAAATGATAA+AGG + chr5.4:12523606-12523625 MS.gene59383:CDS 30.0%
TCGCTTAAACTCTTATAACT+TGG + chr5.4:12520998-12521017 MS.gene59383:intron 30.0%
TGAGATGTTATATTCAGTCT+TGG - chr5.4:12521797-12521816 None:intergenic 30.0%
TTATGAACGTATCTTCACTT+AGG - chr5.4:12523083-12523102 None:intergenic 30.0%
TTCTGTGAGATATAGAAAAG+AGG - chr5.4:12523594-12523613 None:intergenic 30.0%
! AATCAAGCAAACGAATTTTC+AGG - chr5.4:12522978-12522997 None:intergenic 30.0%
! CTCAAGATTGTCTTCTTTTT+CGG - chr5.4:12521884-12521903 None:intergenic 30.0%
! CTGAGAATTTTCATTACTCT+TGG - chr5.4:12522530-12522549 None:intergenic 30.0%
! CTTTTCTATATCTCACAGAA+TGG + chr5.4:12523594-12523613 MS.gene59383:CDS 30.0%
! GTAATTTTGCAGAAATGGTA+TGG + chr5.4:12523053-12523072 MS.gene59383:intron 30.0%
! TCAAGATTGTCTTCTTTTTC+GGG - chr5.4:12521883-12521902 None:intergenic 30.0%
! TGTGTGTAATTTTGCAGAAA+TGG + chr5.4:12523048-12523067 MS.gene59383:intron 30.0%
!!! AGGTTGCATAATAGTTTTGT+TGG + chr5.4:12521351-12521370 MS.gene59383:intron 30.0%
!!! CTAATGACTTTTTCGCTTTT+TGG - chr5.4:12521427-12521446 None:intergenic 30.0%
!!! GGTAGTTCCTTTTTTGTTTT+TGG - chr5.4:12522235-12522254 None:intergenic 30.0%
AAAATTTGCGTGGATCAACT+TGG - chr5.4:12522941-12522960 None:intergenic 35.0%
AACCAATAGTGGTGAAAAAG+TGG + chr5.4:12520799-12520818 MS.gene59383:CDS 35.0%
AATCACCTCCATTAAGAGTT+TGG + chr5.4:12522378-12522397 MS.gene59383:CDS 35.0%
ACAAAGCTTAATTCCCATTC+AGG + chr5.4:12521448-12521467 MS.gene59383:CDS 35.0%
ACCACTGTTTCATAATCAAC+AGG + chr5.4:12521331-12521350 MS.gene59383:intron 35.0%
ATCACCTCCATTAAGAGTTT+GGG + chr5.4:12522379-12522398 MS.gene59383:CDS 35.0%
ATTACTTCTGCAACATTGCT+CGG + chr5.4:12521211-12521230 MS.gene59383:CDS 35.0%
ATTGCTGAAATACTTGAGAG+TGG + chr5.4:12523462-12523481 MS.gene59383:CDS 35.0%
CAAAGCTTAATTCCCATTCA+GGG + chr5.4:12521449-12521468 MS.gene59383:CDS 35.0%
CAGCATCATGAGAATATTTC+CGG + chr5.4:12521109-12521128 MS.gene59383:CDS 35.0%
CATCAGATTCAAGTCATGAT+TGG - chr5.4:12522503-12522522 None:intergenic 35.0%
CATGGAAACTTAATGTTGGT+GGG + chr5.4:12521929-12521948 MS.gene59383:CDS 35.0%
CATTGAAGATCATGAAGTTG+AGG - chr5.4:12522607-12522626 None:intergenic 35.0%
CGAACTAAGGATTCTATTGA+CGG + chr5.4:12521026-12521045 MS.gene59383:intron 35.0%
CTCAACTTCATGATCTTCAA+TGG + chr5.4:12522605-12522624 MS.gene59383:CDS 35.0%
CTTCTTGATTCAGAAGTGAT+AGG + chr5.4:12523114-12523133 MS.gene59383:CDS 35.0%
GACTCCAAATATTGTCATGA+AGG + chr5.4:12522267-12522286 MS.gene59383:CDS 35.0%
GATAAGAAAGTGGATGAATG+AGG + chr5.4:12522195-12522214 MS.gene59383:CDS 35.0%
GTAAGAGAATTGCTTCATCA+AGG + chr5.4:12521716-12521735 MS.gene59383:CDS 35.0%
TAACTTGGATGTTCGAACTA+AGG + chr5.4:12521013-12521032 MS.gene59383:intron 35.0%
TACATCAACCTTGAGGATAT+TGG - chr5.4:12522130-12522149 None:intergenic 35.0%
TAGAGAAGAACCAATTGCAA+GGG + chr5.4:12521979-12521998 MS.gene59383:CDS 35.0%
TCCAGAAAGTTCTTCCATTA+TGG - chr5.4:12523151-12523170 None:intergenic 35.0%
TCTATTCCTGATGAACATCT+TGG - chr5.4:12521152-12521171 None:intergenic 35.0%
TGAAGGACTTGATATCAAAG+TGG + chr5.4:12522284-12522303 MS.gene59383:CDS 35.0%
TGAGTGTGTTACATTTGTTC+TGG + chr5.4:12521664-12521683 MS.gene59383:CDS 35.0%
TTGCTCAAAAATCTGACTAC+TGG - chr5.4:12522724-12522743 None:intergenic 35.0%
! ACAGCTCTGTCTTTGAAAAA+AGG - chr5.4:12523351-12523370 None:intergenic 35.0%
! ACCTGTTGATTATGAAACAG+TGG - chr5.4:12521335-12521354 None:intergenic 35.0%
! ACTTATGCATCCCTTTGTTT+CGG - chr5.4:12523439-12523458 None:intergenic 35.0%
! ATTTTCTTCAGGATGACTCT+TGG - chr5.4:12523736-12523755 None:intergenic 35.0%
! GACATGGACTAATATGATTG+AGG + chr5.4:12522872-12522891 MS.gene59383:intron 35.0%
! TCACCATTTCTGAAGAAACT+AGG + chr5.4:12523771-12523790 MS.gene59383:CDS 35.0%
! TTTATCTTTGCACCTGCTTT+TGG + chr5.4:12523000-12523019 MS.gene59383:intron 35.0%
! TTTGGAGTCAATGTCATATG+AGG - chr5.4:12522256-12522275 None:intergenic 35.0%
!! CATAGAGTTGATGCAAATAG+TGG + chr5.4:12521085-12521104 MS.gene59383:CDS 35.0%
!! GTAGAACTGTTGTTGGTAAT+TGG - chr5.4:12523708-12523727 None:intergenic 35.0%
ACAAGTGTCCAATATCCTCA+AGG + chr5.4:12522119-12522138 MS.gene59383:CDS 40.0%
ACTCCAGAAGCAATGATAAC+AGG - chr5.4:12522157-12522176 None:intergenic 40.0%
ACTTCCCAAACTCTTAATGG+AGG - chr5.4:12522386-12522405 None:intergenic 40.0%
AGGATCATACATCAACCTTG+AGG - chr5.4:12522137-12522156 None:intergenic 40.0%
ATGTCTACTTGGATCAGGAA+TGG - chr5.4:12522329-12522348 None:intergenic 40.0%
ATTGGACACTTGTATTCCTC+AGG - chr5.4:12522112-12522131 None:intergenic 40.0%
CAATGATCCAAGTGACATGT+TGG - chr5.4:12522443-12522462 None:intergenic 40.0%
CAATGATCTGAACTTGCCAA+TGG + chr5.4:12522411-12522430 MS.gene59383:CDS 40.0%
CAATTGACAGATACCATGTG+TGG - chr5.4:12523312-12523331 None:intergenic 40.0%
CTAGAGTTATCCCTTGCAAT+TGG - chr5.4:12521992-12522011 None:intergenic 40.0%
CTTAATTCCCATTCAGGGTA+TGG + chr5.4:12521454-12521473 MS.gene59383:CDS 40.0%
GAGAATATTTCCGGACATAG+AGG + chr5.4:12521118-12521137 MS.gene59383:CDS 40.0%
GATCCTGTTATCATTGCTTC+TGG + chr5.4:12522151-12522170 MS.gene59383:CDS 40.0%
GATCTTACCAACATGTCACT+TGG + chr5.4:12522433-12522452 MS.gene59383:CDS 40.0%
GATTTCTTCGTCGAGAATCA+CGG - chr5.4:12522015-12522034 None:intergenic 40.0%
GCATGGAAACTTAATGTTGG+TGG + chr5.4:12521928-12521947 MS.gene59383:CDS 40.0%
GGAATCTGTAGAACTGTTGT+TGG - chr5.4:12523715-12523734 None:intergenic 40.0%
GTAGAGAAGAACCAATTGCA+AGG + chr5.4:12521978-12521997 MS.gene59383:CDS 40.0%
TAAGACAGAAGTGAGTGCAT+TGG - chr5.4:12523205-12523224 None:intergenic 40.0%
TATGTGTACCTGAAAGGAGA+GGG - chr5.4:12520840-12520859 None:intergenic 40.0%
TATTACTTTCAGGCTGTGAC+AGG + chr5.4:12521380-12521399 MS.gene59383:intron 40.0%
TCTAACTTCAAAGTCGTCCA+CGG - chr5.4:12521756-12521775 None:intergenic 40.0%
TCTGTATGTCTACTTGGATC+AGG - chr5.4:12522334-12522353 None:intergenic 40.0%
TGGACAAACTTCACTGTTGT+AGG - chr5.4:12523292-12523311 None:intergenic 40.0%
TTATGTGTACCTGAAAGGAG+AGG - chr5.4:12520841-12520860 None:intergenic 40.0%
! ATGGAAGAACTTTCTGGAGA+TGG + chr5.4:12523153-12523172 MS.gene59383:CDS 40.0%
! CAACATGTCACTTGGATCAT+TGG + chr5.4:12522441-12522460 MS.gene59383:CDS 40.0%
! GACATGTTGGTAAGATCCAT+TGG - chr5.4:12522430-12522449 None:intergenic 40.0%
! GCCATAATGGAAGAACTTTC+TGG + chr5.4:12523147-12523166 MS.gene59383:CDS 40.0%
! TGCATTGGATGCTGCAATTT+TGG - chr5.4:12523190-12523209 None:intergenic 40.0%
!! AGGAGAAGTACTTGCCATAA+TGG + chr5.4:12523134-12523153 MS.gene59383:CDS 40.0%
!! GGTCTTAACTTGTGACACAT+CGG - chr5.4:12522482-12522501 None:intergenic 40.0%
!!! CTCCACTTTTTCACCACTAT+TGG - chr5.4:12520804-12520823 None:intergenic 40.0%
!!! GTCTTCTTTTTCGGGTCATT+TGG - chr5.4:12521875-12521894 None:intergenic 40.0%
ACTGGAACCATACCCTGAAT+GGG - chr5.4:12521464-12521483 None:intergenic 45.0%
ATTCTGCTGAAGAAGAGGCT+GGG + chr5.4:12521687-12521706 MS.gene59383:CDS 45.0%
CACATGCATCAAAACCCAGA+AGG + chr5.4:12523814-12523833 MS.gene59383:CDS 45.0%
CAGTGAAGTTTGTCCACACA+TGG + chr5.4:12523296-12523315 MS.gene59383:CDS 45.0%
CTGACACTGACAAAGTAGCT+TGG - chr5.4:12522684-12522703 None:intergenic 45.0%
GAGCAATGCAAATGATCAGC+AGG + chr5.4:12522738-12522757 MS.gene59383:CDS 45.0%
GATGAATCAGTCGGATAGAG+TGG + chr5.4:12522081-12522100 MS.gene59383:CDS 45.0%
GCATGAACTGTATGCTTGTG+AGG - chr5.4:12522553-12522572 None:intergenic 45.0%
TCCAAATACCCTCTCCTTTC+AGG + chr5.4:12520829-12520848 MS.gene59383:CDS 45.0%
TCTGGATTCTGCTGAAGAAG+AGG + chr5.4:12521682-12521701 MS.gene59383:CDS 45.0%
! AATGTTGGTGGGAGAACACA+TGG + chr5.4:12521940-12521959 MS.gene59383:CDS 45.0%
!! ACCTGAAAGGAGAGGGTATT+TGG - chr5.4:12520833-12520852 None:intergenic 45.0%
!! CTTCTGGGTTTTGATGCATG+TGG - chr5.4:12523816-12523835 None:intergenic 45.0%
!! GTTGTTGGTAATTGGCTGAG+AGG - chr5.4:12523700-12523719 None:intergenic 45.0%
!! TAAAGGAAAGGAAAGTGCCG+TGG + chr5.4:12523623-12523642 MS.gene59383:CDS 45.0%
AAAGTCGTCCACGGAATCTG+GGG - chr5.4:12521747-12521766 None:intergenic 50.0%
ACCTCTACAGCCAACATGAC+TGG - chr5.4:12521482-12521501 None:intergenic 50.0%
AGTCCATGTCGGACAACCTT+AGG - chr5.4:12522862-12522881 None:intergenic 50.0%
CAAAGTCGTCCACGGAATCT+GGG - chr5.4:12521748-12521767 None:intergenic 50.0%
GACTGGAACCATACCCTGAA+TGG - chr5.4:12521465-12521484 None:intergenic 50.0%
GATTCTGCTGAAGAAGAGGC+TGG + chr5.4:12521686-12521705 MS.gene59383:CDS 50.0%
GGAATGCAGCAAACTCTACC+TGG + chr5.4:12521232-12521251 MS.gene59383:CDS 50.0%
GGACACTTGTATTCCTCAGG+TGG - chr5.4:12522109-12522128 None:intergenic 50.0%
GGGTATGGTTCCAGTCATGT+TGG + chr5.4:12521469-12521488 MS.gene59383:CDS 50.0%
GTTCCTAAGGTTGTCCGACA+TGG + chr5.4:12522856-12522875 MS.gene59383:intron 50.0%
TCAAAGTCGTCCACGGAATC+TGG - chr5.4:12521749-12521768 None:intergenic 50.0%
TGCGTGGATCAACTTGGTTG+CGG - chr5.4:12522935-12522954 None:intergenic 50.0%
TTCCGACAGAAGCTGAGTTC+CGG - chr5.4:12522782-12522801 None:intergenic 50.0%
TTCCGGACATAGAGGAAGCA+CGG + chr5.4:12521126-12521145 MS.gene59383:CDS 50.0%
! TCCAGTCATGTTGGCTGTAG+AGG + chr5.4:12521478-12521497 MS.gene59383:CDS 50.0%
AGCCGGAACTCAGCTTCTGT+CGG + chr5.4:12522777-12522796 MS.gene59383:CDS 55.0%
AGGTTCAACCCCAGATTCCG+TGG + chr5.4:12521736-12521755 MS.gene59383:CDS 55.0%
GCACGGCCAAGATGTTCATC+AGG + chr5.4:12521143-12521162 MS.gene59383:CDS 55.0%
!! GACGTAAAAGCGCTGAGAGC+CGG + chr5.4:12522760-12522779 MS.gene59383:CDS 55.0%
GGATAGAGTGGCACCACCTG+AGG + chr5.4:12522093-12522112 MS.gene59383:CDS 60.0%
GGCCGTGCTTCCTCTATGTC+CGG - chr5.4:12521131-12521150 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 12520794 12523839 12520794 ID=MS.gene59383
chr5.4 mRNA 12520794 12523839 12520794 ID=MS.gene59383.t1;Parent=MS.gene59383
chr5.4 exon 12520794 12520850 12520794 ID=MS.gene59383.t1.exon1;Parent=MS.gene59383.t1
chr5.4 CDS 12520794 12520850 12520794 ID=cds.MS.gene59383.t1;Parent=MS.gene59383.t1
chr5.4 exon 12521068 12521253 12521068 ID=MS.gene59383.t1.exon2;Parent=MS.gene59383.t1
chr5.4 CDS 12521068 12521253 12521068 ID=cds.MS.gene59383.t1;Parent=MS.gene59383.t1
chr5.4 exon 12521392 12521499 12521392 ID=MS.gene59383.t1.exon3;Parent=MS.gene59383.t1
chr5.4 CDS 12521392 12521499 12521392 ID=cds.MS.gene59383.t1;Parent=MS.gene59383.t1
chr5.4 exon 12521641 12522812 12521641 ID=MS.gene59383.t1.exon4;Parent=MS.gene59383.t1
chr5.4 CDS 12521641 12522812 12521641 ID=cds.MS.gene59383.t1;Parent=MS.gene59383.t1
chr5.4 exon 12523065 12523839 12523065 ID=MS.gene59383.t1.exon5;Parent=MS.gene59383.t1
chr5.4 CDS 12523065 12523839 12523065 ID=cds.MS.gene59383.t1;Parent=MS.gene59383.t1
Gene Sequence

>MS.gene59383

ATGAGAACCAATAGTGGTGAAAAAGTGGAGACTTTTCCAAATACCCTCTCCTTTCAGGTACACATAAATAAATACCATTGCATTTCACTATTTTGTTCATCATATCATAGGTGTTTCTGAATAACAAACTCTTAACAGAACTAACCAATCTAACTAACAAACGATATCTGATTTGTAACTGATTTGTCGTTCATTCACATGTCTTCGCTTAAACTCTTATAACTTGGATGTTCGAACTAAGGATTCTATTGACGGAATTTATGTTAATTAACAGGTACATAGTAAAATATGCATAGAGTTGATGCAAATAGTGGACAGCATCATGAGAATATTTCCGGACATAGAGGAAGCACGGCCAAGATGTTCATCAGGAATAGAGTCTCTATGTTTTCTGAACAACTCAATTGAGAAAGCTAAATTACTTCTGCAACATTGCTCGGAATGCAGCAAACTCTACCTGGTGTGTATTTTTTTTTCCTTTCTAAAAACATAAAGAAATTAACTTGCATAACTTTCTGAACAAGTCAATGAAATATGACCACTGTTTCATAATCAACAGGTTGCATAATAGTTTTGTTGGTTTCAATATTACTTTCAGGCTGTGACAGGAGAAACAGTACTTTCGAGATGCCAAAAAGCGAAAAAGTCATTAGAACAAAGCTTAATTCCCATTCAGGGTATGGTTCCAGTCATGTTGGCTGTAGAGGTTAGTCAATTTTTTCTTCATCATCTAACTTTTTTGCAAGTCTACGTGCTTTTTCATAACTAAAAATTAAGTATTTATTGTACTTGAAAAAAATTCTATAAACAAATTAATCCAAATTGAGTTTTATATTTTCTGTTGTAGGTTTCTAGAATAATTGATGATCTTGAGTGTGTTACATTTGTTCTGGATTCTGCTGAAGAAGAGGCTGGGAGAGTTGTAAGAGAATTGCTTCATCAAGGTTCAACCCCAGATTCCGTGGACGACTTTGAAGTTAGAGCTCTTCAGTTTGCAGCACCAAGACTGAATATAACATCTCAAAAGGCCATTTTAATCGAGAAACGATCTATCAAGAAGTTTCTAGATAAAGTTGGACCAAATGACCCGAAAAAGAAGACAATCTTGAGATATCTTATGTATCTAATGAAAAAGCATGGAAACTTAATGTTGGTGGGAGAACACATGGATAATTTTTATTCTCGTAGAGAAGAACCAATTGCAAGGGATAACTCTAGCCGTGATTCTCGACGAAGAAATCGTGTTGAGTCAGATAATAGCATGAACTATGATCAGCATAAAACTGAGATGAATCAGTCGGATAGAGTGGCACCACCTGAGGAATACAAGTGTCCAATATCCTCAAGGTTGATGTATGATCCTGTTATCATTGCTTCTGGAGTAACATATGAAAGAATGTGGATAAGAAAGTGGATGAATGAGGGTAAAACTATATGTCCAAAAACAAAAAAGGAACTACCTCATATGACATTGACTCCAAATATTGTCATGAAGGACTTGATATCAAAGTGGTGCAAAAATAATGGTGTCTCCATTCCTGATCCAAGTAGACATACAGAAGATTTTATGTTGTTGGATGCTTCAATCACCTCCATTAAGAGTTTGGGAAGTTATTTCAATGATCTGAACTTGCCAATGGATCTTACCAACATGTCACTTGGATCATTGGATAATAGTTTTAGTTCCGATGTGTCACAAGTTAAGACCAATCATGACTTGAATCTGATGATGACCAAGAGTAATGAAAATTCTCAGCCTCACAAGCATACAGTTCATGCAGAGATACATGATACAGATTTGATGCTCTTGCCTCAACTTCATGATCTTCAATGGGATTCTCAATGTAAGATCATTGAAGATTTGAAAGATCATATGAAAAGCAATAGCCAAGCTACTTTGTCAGTGTCAGCTGAAAATTTAGTTGAACCAGTAGTCAGATTTTTGAGCAATGCAAATGATCAGCAGGACGTAAAAGCGCTGAGAGCCGGAACTCAGCTTCTGTCGGAATTTGTGAAATTGTAGAAAGGAGCTAATTTAGTCTATAAAACTATAAATAATTAAGTTCCTAAGGTTGTCCGACATGGACTAATATGATTGAGGATTTCAAATTTCAGACTCTTTTGAATATTAATCATTTCCGCAACCAAGTTGATCCACGCAAATTTTAAGTTTCTTGCAGACCTGAAAATTCGTTTGCTTGATTGCTTTATCTTTGCACCTGCTTTTGGTATTATAAATTTATAATGACTTTTTTGTGTGTAATTTTGCAGAAATGGTATGGCTAACCTAAGTGAAGATACGTTCATAAAGTTGACAAATCTTCTTGATTCAGAAGTGATAGGAGAAGTACTTGCCATAATGGAAGAACTTTCTGGAGATGGAAATAGTAAAGCCAAAATTGCAGCATCCAATGCACTCACTTCTGTCTTAAAGTTACTTGATTCTGATAACAAAGGGTTCCAACAACATGCTATAAGAATAATCTACAATTTGTCCTACAACAGTGAAGTTTGTCCACACATGGTATCTGTCAATTGCATCCCAAAATTGTTACCTTTTTTCAAAGACAGAGCTGTTTTGAGATACTGCATATATATATTGAAAAACATTTGTGATACTGAAGAAGGTAGAAATTCTATTGCCGAAACAAAGGGATGCATAAGTTCTATTGCTGAAATACTTGAGAGTGGAAGTAATGAAGAGCAAGAACATGCACTTGATGTTCTACTATCTTTATGTACATGTTCTCAAAATGTGGATTATCTTAAGTTGATTTTGGATGAAGATATTATCACTCCTCTTTTCTATATCTCACAGAATGGAAATGATAAAGGAAAGGAAAGTGCCGTGGAATTGCTTCATATTTTGAGAGATGCAAAATGTGTTGAGAATGAAGATAGGTCCTCTCAGCCAATTACCAACAACAGTTCTACAGATTCCAAGAGTCATCCTGAAGAAAATAGATCATCAAAGAAATCACCATTTCTGAAGAAACTAGGATTGTTCTCGAAATCTACTCCACATGCATCAAAACCCAGAAGGTGA
Protein sequence

>MS.gene59383.t1

MRTNSGEKVETFPNTLSFQVHSKICIELMQIVDSIMRIFPDIEEARPRCSSGIESLCFLNNSIEKAKLLLQHCSECSKLYLAVTGETVLSRCQKAKKSLEQSLIPIQGMVPVMLAVEVSRIIDDLECVTFVLDSAEEEAGRVVRELLHQGSTPDSVDDFEVRALQFAAPRLNITSQKAILIEKRSIKKFLDKVGPNDPKKKTILRYLMYLMKKHGNLMLVGEHMDNFYSRREEPIARDNSSRDSRRRNRVESDNSMNYDQHKTEMNQSDRVAPPEEYKCPISSRLMYDPVIIASGVTYERMWIRKWMNEGKTICPKTKKELPHMTLTPNIVMKDLISKWCKNNGVSIPDPSRHTEDFMLLDASITSIKSLGSYFNDLNLPMDLTNMSLGSLDNSFSSDVSQVKTNHDLNLMMTKSNENSQPHKHTVHAEIHDTDLMLLPQLHDLQWDSQCKIIEDLKDHMKSNSQATLSVSAENLVEPVVRFLSNANDQQDVKALRAGTQLLSEFVKLNGMANLSEDTFIKLTNLLDSEVIGEVLAIMEELSGDGNSKAKIAASNALTSVLKLLDSDNKGFQQHAIRIIYNLSYNSEVCPHMVSVNCIPKLLPFFKDRAVLRYCIYILKNICDTEEGRNSIAETKGCISSIAEILESGSNEEQEHALDVLLSLCTCSQNVDYLKLILDEDIITPLFYISQNGNDKGKESAVELLHILRDAKCVENEDRSSQPITNNSSTDSKSHPEENRSSKKSPFLKKLGLFSKSTPHASKPRR