Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058650.t1 | XP_003623996.1 | 99.4 | 154 | 1 | 0 | 1 | 154 | 105 | 258 | 3.50E-81 | 310.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058650.t1 | Q1KLZ1 | 91.6 | 154 | 13 | 0 | 1 | 154 | 100 | 253 | 3.1e-78 | 292.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058650.t1 | G7L5N5 | 99.4 | 154 | 1 | 0 | 1 | 154 | 12 | 165 | 2.5e-81 | 310.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052637 | MS.gene058650 | 0.813581 | 2.38E-51 | -1.69E-46 |
| MS.gene054826 | MS.gene058650 | 0.880493 | 5.25E-70 | -1.69E-46 |
| MS.gene058650 | MS.gene058651 | 0.951749 | 1.06E-109 | -1.69E-46 |
| MS.gene058650 | MS.gene059030 | 0.80675 | 7.07E-50 | -1.69E-46 |
| MS.gene058650 | MS.gene066921 | 0.804497 | 2.10E-49 | -1.69E-46 |
| MS.gene058650 | MS.gene070915 | 0.811933 | 5.46E-51 | -1.69E-46 |
| MS.gene058650 | MS.gene24016 | 0.811139 | 8.13E-51 | -1.69E-46 |
| MS.gene058650 | MS.gene25967 | 0.816024 | 6.85E-52 | -1.69E-46 |
| MS.gene058650 | MS.gene25968 | 0.842327 | 2.77E-58 | -1.69E-46 |
| MS.gene058650 | MS.gene29360 | 0.814592 | 1.42E-51 | -1.69E-46 |
| MS.gene058650 | MS.gene36309 | 0.800069 | 1.71E-48 | -1.69E-46 |
| MS.gene058650 | MS.gene52446 | 0.96818 | 2.60E-128 | -1.69E-46 |
| MS.gene058650 | MS.gene59383 | 0.809593 | 1.75E-50 | -1.69E-46 |
| MS.gene058650 | MS.gene70630 | 0.806041 | 9.97E-50 | -1.69E-46 |
| MS.gene058650 | MS.gene72782 | 0.801714 | 7.91E-49 | -1.69E-46 |
| MS.gene058650 | MS.gene81581 | 0.800562 | 1.36E-48 | -1.69E-46 |
| MS.gene058650 | MS.gene82148 | 0.801123 | 1.04E-48 | -1.69E-46 |
| MS.gene058650 | MS.gene94815 | 0.816435 | 5.54E-52 | -1.69E-46 |
| MS.gene058650 | MS.gene96454 | 0.804851 | 1.77E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 54 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGAATCAGTTTGGCATTTA+TGG | 0.111575 | 7.2:-27717847 | MS.gene058650:CDS |
| GAGAAAATCACCTTAACATT+AGG | 0.229682 | 7.2:+27717182 | None:intergenic |
| GGTAGCGGAGGCACGGTTTC+CGG | 0.231220 | 7.2:-27717233 | MS.gene058650:CDS |
| CACTGTTCCTCCCATTCCTT+TGG | 0.269450 | 7.2:+27717716 | None:intergenic |
| GTAGCGGAGGCACGGTTTCC+GGG | 0.287571 | 7.2:-27717232 | MS.gene058650:CDS |
| AGAAAATCACCTTAACATTA+GGG | 0.297633 | 7.2:+27717183 | None:intergenic |
| AACCTTACCTTAGTATTGGC+TGG | 0.308983 | 7.2:+27717630 | None:intergenic |
| AAACAACAGGTCCTGAGATA+TGG | 0.318320 | 7.2:-27717298 | MS.gene058650:CDS |
| AGGGTTTGTATGAGAGCATT+TGG | 0.332544 | 7.2:-27717765 | MS.gene058650:CDS |
| TCGATTCAACTAACCTTTCC+AGG | 0.333739 | 7.2:+27717982 | None:intergenic |
| GACATATTTGTGATGGGAAT+AGG | 0.357383 | 7.2:-27717254 | MS.gene058650:CDS |
| GTTCCTCCCATTCCTTTGGT+TGG | 0.365478 | 7.2:+27717720 | None:intergenic |
| AATGGGAGGAACAGTGAAAA+AGG | 0.372245 | 7.2:-27717709 | MS.gene058650:CDS |
| TGTTGCAGCATAAAAGCATT+TGG | 0.385420 | 7.2:+27717663 | None:intergenic |
| AACCATCTTATATCCCTTCA+TGG | 0.389444 | 7.2:+27717821 | None:intergenic |
| CTTTCCAAGCTTGTGTAAGA+TGG | 0.401441 | 7.2:+27717789 | None:intergenic |
| GGCATTTATGGCTGCCATGA+AGG | 0.421016 | 7.2:-27717835 | MS.gene058650:CDS |
| ATCACAAAACCCTAATGTTA+AGG | 0.422500 | 7.2:-27717192 | MS.gene058650:CDS |
| CTTACACAAGCTTGGAAAGA+AGG | 0.427704 | 7.2:-27717785 | MS.gene058650:CDS |
| GGAGGCACGGTTTCCGGGGT+TGG | 0.433673 | 7.2:-27717227 | MS.gene058650:CDS |
| TGTTAACCTTACCTTAGTAT+TGG | 0.438994 | 7.2:+27717626 | None:intergenic |
| CAGACCGGCACTTGCTATGA+TGG | 0.446594 | 7.2:-27718034 | MS.gene058650:CDS |
| AGGCAACATGGGAATCAGTT+TGG | 0.448530 | 7.2:-27717856 | MS.gene058650:CDS |
| TACCTTAGTATTGGCTGGGT+TGG | 0.454890 | 7.2:+27717635 | None:intergenic |
| ACCTTACCTTAGTATTGGCT+GGG | 0.467817 | 7.2:+27717631 | None:intergenic |
| TTCCAACCCAGCCAATACTA+AGG | 0.471629 | 7.2:-27717637 | MS.gene058650:intron |
| ATTCCCATGTTGCCTGATGT+TGG | 0.472301 | 7.2:+27717864 | None:intergenic |
| AACAACAGGTCCTGAGATAT+GGG | 0.481398 | 7.2:-27717297 | MS.gene058650:CDS |
| CACTGATCCAACCAAAGGAA+TGG | 0.496426 | 7.2:-27717727 | MS.gene058650:CDS |
| TTTGTATCCTCCCATATCTC+AGG | 0.504891 | 7.2:+27717287 | None:intergenic |
| GCATTTATGGCTGCCATGAA+GGG | 0.510121 | 7.2:-27717834 | MS.gene058650:CDS |
| ACTGATCCAACCAAAGGAAT+GGG | 0.516662 | 7.2:-27717726 | MS.gene058650:CDS |
| TATGCCATCTTACACAAGCT+TGG | 0.524193 | 7.2:-27717793 | MS.gene058650:CDS |
| TTACACAAGCTTGGAAAGAA+GGG | 0.524356 | 7.2:-27717784 | MS.gene058650:CDS |
| GAGATATGGGAGGATACAAA+TGG | 0.524396 | 7.2:-27717284 | MS.gene058650:CDS |
| CATTTGGTGTGGATTCCAAA+AGG | 0.529158 | 7.2:+27717679 | None:intergenic |
| ATTCTGATAGAGCCAACATC+AGG | 0.532002 | 7.2:-27717876 | MS.gene058650:CDS |
| TAGCGGAGGCACGGTTTCCG+GGG | 0.556029 | 7.2:-27717231 | MS.gene058650:CDS |
| TGCCATGAAGGGATATAAGA+TGG | 0.557496 | 7.2:-27717823 | MS.gene058650:CDS |
| CAGGTGCATTTCGAAACAAC+AGG | 0.558100 | 7.2:-27717311 | MS.gene058650:CDS |
| AGAGCCAACATCAGGCAACA+TGG | 0.563901 | 7.2:-27717868 | MS.gene058650:CDS |
| GAATTTGATAACTCCTGGAA+AGG | 0.576005 | 7.2:-27717995 | MS.gene058650:intron |
| CAGCATAAAAGCATTTGGTG+TGG | 0.576924 | 7.2:+27717668 | None:intergenic |
| ACAAGTTGACATATTTGTGA+TGG | 0.584399 | 7.2:-27717261 | MS.gene058650:CDS |
| CAAGTTGACATATTTGTGAT+GGG | 0.592268 | 7.2:-27717260 | MS.gene058650:CDS |
| GGGAATAGGTAGCGGAGGCA+CGG | 0.600122 | 7.2:-27717240 | MS.gene058650:CDS |
| TCTTCCATCATAGCAAGTGC+CGG | 0.604090 | 7.2:+27718030 | None:intergenic |
| GAGCCAACATCAGGCAACAT+GGG | 0.610601 | 7.2:-27717867 | MS.gene058650:CDS |
| TTTGTGATGGGAATAGGTAG+CGG | 0.619890 | 7.2:-27717248 | MS.gene058650:CDS |
| ATTCTCACTGATCCAACCAA+AGG | 0.629727 | 7.2:-27717732 | MS.gene058650:CDS |
| TTTAAGATATTGTCCAACCC+CGG | 0.638530 | 7.2:+27717214 | None:intergenic |
| GATCCAACCAAAGGAATGGG+AGG | 0.667757 | 7.2:-27717723 | MS.gene058650:CDS |
| GTGATGGGAATAGGTAGCGG+AGG | 0.681582 | 7.2:-27717245 | MS.gene058650:CDS |
| AACAGGTCCTGAGATATGGG+AGG | 0.695778 | 7.2:-27717294 | MS.gene058650:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATACATAACTTACGTTAAAA+TGG | - | chr7.2:27717870-27717889 | MS.gene058650:CDS | 20.0% |
| !! | TACATAACTTACGTTAAAAT+GGG | - | chr7.2:27717871-27717890 | MS.gene058650:CDS | 20.0% |
| ! | AGAAAATCACCTTAACATTA+GGG | + | chr7.2:27718029-27718048 | None:intergenic | 25.0% |
| ! | TACTATTTATGTTCGAAACA+GGG | - | chr7.2:27717619-27717638 | MS.gene058650:intron | 25.0% |
| ! | TTCATGTTTGTGTTAATTGA+TGG | - | chr7.2:27717302-27717321 | MS.gene058650:CDS | 25.0% |
| !! | AAAAAGAATTTGATAACTCC+TGG | - | chr7.2:27717209-27717228 | MS.gene058650:CDS | 25.0% |
| ATCACAAAACCCTAATGTTA+AGG | - | chr7.2:27718017-27718036 | MS.gene058650:CDS | 30.0% | |
| ATGTAAACAACGAGCAAAAT+TGG | + | chr7.2:27717267-27717286 | None:intergenic | 30.0% | |
| GAGAAAATCACCTTAACATT+AGG | + | chr7.2:27718030-27718049 | None:intergenic | 30.0% | |
| GGATCGTAAAAAATAGTAGT+TGG | + | chr7.2:27717772-27717791 | None:intergenic | 30.0% | |
| GTACTATTTATGTTCGAAAC+AGG | - | chr7.2:27717618-27717637 | MS.gene058650:intron | 30.0% | |
| TGTTAACCTTACCTTAGTAT+TGG | + | chr7.2:27717586-27717605 | None:intergenic | 30.0% | |
| TTACGTTAAAATGGGAAAAC+AGG | - | chr7.2:27717879-27717898 | MS.gene058650:CDS | 30.0% | |
| TTGTTAGATAGTAGCTATGA+CGG | - | chr7.2:27717642-27717661 | MS.gene058650:CDS | 30.0% | |
| ! | ACAAGTTGACATATTTGTGA+TGG | - | chr7.2:27717948-27717967 | MS.gene058650:intron | 30.0% |
| ! | CAAGTTGACATATTTGTGAT+GGG | - | chr7.2:27717949-27717968 | MS.gene058650:intron | 30.0% |
| AACCATCTTATATCCCTTCA+TGG | + | chr7.2:27717391-27717410 | None:intergenic | 35.0% | |
| TAAACAACGAGCAAAATTGG+TGG | + | chr7.2:27717264-27717283 | None:intergenic | 35.0% | |
| TTACACAAGCTTGGAAAGAA+GGG | - | chr7.2:27717425-27717444 | MS.gene058650:intron | 35.0% | |
| ! | AAACCAGTGTTTGTTAACCA+CGG | + | chr7.2:27717793-27717812 | None:intergenic | 35.0% |
| ! | GAAAAAGGCTTATGACCTTT+TGG | - | chr7.2:27717515-27717534 | MS.gene058650:intron | 35.0% |
| ! | GAATTTGATAACTCCTGGAA+AGG | - | chr7.2:27717214-27717233 | MS.gene058650:CDS | 35.0% |
| ! | GACATATTTGTGATGGGAAT+AGG | - | chr7.2:27717955-27717974 | MS.gene058650:intron | 35.0% |
| ! | TTTAAGATATTGTCCAACCC+CGG | + | chr7.2:27717998-27718017 | None:intergenic | 35.0% |
| !! | TGTTGCAGCATAAAAGCATT+TGG | + | chr7.2:27717549-27717568 | None:intergenic | 35.0% |
| !!! | CTACTATTTTTTACGATCCG+TGG | - | chr7.2:27717773-27717792 | MS.gene058650:CDS | 35.0% |
| AAACAACAGGTCCTGAGATA+TGG | - | chr7.2:27717911-27717930 | MS.gene058650:intron | 40.0% | |
| AACAACAGGTCCTGAGATAT+GGG | - | chr7.2:27717912-27717931 | MS.gene058650:intron | 40.0% | |
| AATGGGAGGAACAGTGAAAA+AGG | - | chr7.2:27717500-27717519 | MS.gene058650:intron | 40.0% | |
| ACTGATCCAACCAAAGGAAT+GGG | - | chr7.2:27717483-27717502 | MS.gene058650:intron | 40.0% | |
| ATTCTCACTGATCCAACCAA+AGG | - | chr7.2:27717477-27717496 | MS.gene058650:intron | 40.0% | |
| ATTCTGATAGAGCCAACATC+AGG | - | chr7.2:27717333-27717352 | MS.gene058650:intron | 40.0% | |
| CCATCAAATAGTGACTAGAG+CGG | - | chr7.2:27717714-27717733 | MS.gene058650:CDS | 40.0% | |
| CTTACACAAGCTTGGAAAGA+AGG | - | chr7.2:27717424-27717443 | MS.gene058650:intron | 40.0% | |
| CTTTCCAAGCTTGTGTAAGA+TGG | + | chr7.2:27717423-27717442 | None:intergenic | 40.0% | |
| GAGATATGGGAGGATACAAA+TGG | - | chr7.2:27717925-27717944 | MS.gene058650:intron | 40.0% | |
| GGGAATCAGTTTGGCATTTA+TGG | - | chr7.2:27717362-27717381 | MS.gene058650:intron | 40.0% | |
| TATGCCATCTTACACAAGCT+TGG | - | chr7.2:27717416-27717435 | MS.gene058650:intron | 40.0% | |
| TCGATTCAACTAACCTTTCC+AGG | + | chr7.2:27717230-27717249 | None:intergenic | 40.0% | |
| TGCCATGAAGGGATATAAGA+TGG | - | chr7.2:27717386-27717405 | MS.gene058650:intron | 40.0% | |
| TTTGTATCCTCCCATATCTC+AGG | + | chr7.2:27717925-27717944 | None:intergenic | 40.0% | |
| ! | TTTGTGATGGGAATAGGTAG+CGG | - | chr7.2:27717961-27717980 | MS.gene058650:intron | 40.0% |
| !! | AACCTTACCTTAGTATTGGC+TGG | + | chr7.2:27717582-27717601 | None:intergenic | 40.0% |
| !! | ACCTTACCTTAGTATTGGCT+GGG | + | chr7.2:27717581-27717600 | None:intergenic | 40.0% |
| !! | AGGGTTTGTATGAGAGCATT+TGG | - | chr7.2:27717444-27717463 | MS.gene058650:intron | 40.0% |
| !! | CAGCATAAAAGCATTTGGTG+TGG | + | chr7.2:27717544-27717563 | None:intergenic | 40.0% |
| !! | CATTTGGTGTGGATTCCAAA+AGG | + | chr7.2:27717533-27717552 | None:intergenic | 40.0% |
| ACCCAGCCAATACTAAGGTA+AGG | - | chr7.2:27717577-27717596 | MS.gene058650:intron | 45.0% | |
| AGGCAACATGGGAATCAGTT+TGG | - | chr7.2:27717353-27717372 | MS.gene058650:intron | 45.0% | |
| ATTCCCATGTTGCCTGATGT+TGG | + | chr7.2:27717348-27717367 | None:intergenic | 45.0% | |
| CACTGATCCAACCAAAGGAA+TGG | - | chr7.2:27717482-27717501 | MS.gene058650:intron | 45.0% | |
| CAGGTGCATTTCGAAACAAC+AGG | - | chr7.2:27717898-27717917 | MS.gene058650:intron | 45.0% | |
| CCGCTCTAGTCACTATTTGA+TGG | + | chr7.2:27717717-27717736 | None:intergenic | 45.0% | |
| GATCCGTGGTTAACAAACAC+TGG | - | chr7.2:27717787-27717806 | MS.gene058650:CDS | 45.0% | |
| GCATTTATGGCTGCCATGAA+GGG | - | chr7.2:27717375-27717394 | MS.gene058650:intron | 45.0% | |
| TCTTCCATCATAGCAAGTGC+CGG | + | chr7.2:27717182-27717201 | None:intergenic | 45.0% | |
| TTCCAACCCAGCCAATACTA+AGG | - | chr7.2:27717572-27717591 | MS.gene058650:intron | 45.0% | |
| ! | AACGAGCAAAATTGGTGGCA+TGG | + | chr7.2:27717259-27717278 | None:intergenic | 45.0% |
| !! | TACCTTAGTATTGGCTGGGT+TGG | + | chr7.2:27717577-27717596 | None:intergenic | 45.0% |
| AACAGGTCCTGAGATATGGG+AGG | - | chr7.2:27717915-27717934 | MS.gene058650:intron | 50.0% | |
| AGAGCCAACATCAGGCAACA+TGG | - | chr7.2:27717341-27717360 | MS.gene058650:intron | 50.0% | |
| CACTGTTCCTCCCATTCCTT+TGG | + | chr7.2:27717496-27717515 | None:intergenic | 50.0% | |
| GAGCCAACATCAGGCAACAT+GGG | - | chr7.2:27717342-27717361 | MS.gene058650:intron | 50.0% | |
| GATCCAACCAAAGGAATGGG+AGG | - | chr7.2:27717486-27717505 | MS.gene058650:intron | 50.0% | |
| GGCATTTATGGCTGCCATGA+AGG | - | chr7.2:27717374-27717393 | MS.gene058650:intron | 50.0% | |
| GTTCCTCCCATTCCTTTGGT+TGG | + | chr7.2:27717492-27717511 | None:intergenic | 50.0% | |
| ! | GTGATGGGAATAGGTAGCGG+AGG | - | chr7.2:27717964-27717983 | MS.gene058650:intron | 55.0% |
| !! | GGGAATAGGTAGCGGAGGCA+CGG | - | chr7.2:27717969-27717988 | MS.gene058650:intron | 60.0% |
| ! | GTAGCGGAGGCACGGTTTCC+GGG | - | chr7.2:27717977-27717996 | MS.gene058650:intron | 65.0% |
| ! | TAGCGGAGGCACGGTTTCCG+GGG | - | chr7.2:27717978-27717997 | MS.gene058650:intron | 65.0% |
| !! | GGTAGCGGAGGCACGGTTTC+CGG | - | chr7.2:27717976-27717995 | MS.gene058650:intron | 65.0% |
| ! | GGAGGCACGGTTTCCGGGGT+TGG | - | chr7.2:27717982-27718001 | MS.gene058650:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 27717178 | 27718053 | 27717178 | ID=MS.gene058650 |
| chr7.2 | mRNA | 27717178 | 27718053 | 27717178 | ID=MS.gene058650.t1;Parent=MS.gene058650 |
| chr7.2 | exon | 27717996 | 27718053 | 27717996 | ID=MS.gene058650.t1.exon1;Parent=MS.gene058650.t1 |
| chr7.2 | CDS | 27717996 | 27718053 | 27717996 | ID=cds.MS.gene058650.t1;Parent=MS.gene058650.t1 |
| chr7.2 | exon | 27717638 | 27717901 | 27717638 | ID=MS.gene058650.t1.exon2;Parent=MS.gene058650.t1 |
| chr7.2 | CDS | 27717638 | 27717901 | 27717638 | ID=cds.MS.gene058650.t1;Parent=MS.gene058650.t1 |
| chr7.2 | exon | 27717178 | 27717330 | 27717178 | ID=MS.gene058650.t1.exon3;Parent=MS.gene058650.t1 |
| chr7.2 | CDS | 27717178 | 27717330 | 27717178 | ID=cds.MS.gene058650.t1;Parent=MS.gene058650.t1 |
| Gene Sequence |
| Protein sequence |
