Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59402.t1 | XP_003612062.1 | 93.6 | 125 | 8 | 0 | 1 | 125 | 1 | 125 | 3.90E-59 | 237.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59402.t1 | G7KF00 | 93.6 | 125 | 8 | 0 | 1 | 125 | 1 | 125 | 2.8e-59 | 237.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056530 | MS.gene59402 | 0.827016 | 1.97E-54 | -1.69E-46 |
| MS.gene059514 | MS.gene59402 | 0.802698 | 4.96E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59402.t1 | MTR_5g020860 | 93.600 | 125 | 8 | 0 | 1 | 125 | 1 | 125 | 2.39e-84 | 242 |
| MS.gene59402.t1 | MTR_8g077145 | 62.992 | 127 | 41 | 4 | 1 | 125 | 1 | 123 | 2.02e-49 | 154 |
| MS.gene59402.t1 | MTR_7g026910 | 71.429 | 91 | 5 | 1 | 1 | 91 | 1 | 70 | 6.27e-37 | 120 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59402.t1 | AT3G50900 | 40.741 | 135 | 49 | 5 | 1 | 125 | 1 | 114 | 1.46e-21 | 83.2 |
| MS.gene59402.t1 | AT2G18210 | 44.898 | 98 | 42 | 4 | 33 | 125 | 34 | 124 | 5.36e-19 | 77.0 |
| MS.gene59402.t1 | AT4G36500 | 43.011 | 93 | 49 | 1 | 33 | 125 | 34 | 122 | 6.77e-16 | 68.9 |
| MS.gene59402.t1 | AT5G66490 | 33.835 | 133 | 56 | 5 | 1 | 125 | 1 | 109 | 6.73e-13 | 60.8 |
Find 32 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGAATTTGGTGGAAATAA+TGG | 0.233549 | 5.4:+12752100 | MS.gene59402:CDS |
| ATCAAATACAAAGGTAAATA+TGG | 0.271944 | 5.4:+12752174 | MS.gene59402:CDS |
| CACTACTTGCTTTCTTTGTT+GGG | 0.272849 | 5.4:-12752237 | None:intergenic |
| GAGAAATTAGATAAAGAATT+TGG | 0.286753 | 5.4:+12752088 | MS.gene59402:CDS |
| TCAACATATCTTGCAAATTC+AGG | 0.290589 | 5.4:-12752337 | None:intergenic |
| ATATTTACCTTTGTATTTGA+TGG | 0.294161 | 5.4:-12752172 | None:intergenic |
| ATGGCCTCTTCTAGTACTAT+TGG | 0.297989 | 5.4:-12752056 | None:intergenic |
| TCACTACTTGCTTTCTTTGT+TGG | 0.309163 | 5.4:-12752238 | None:intergenic |
| TACAAAGGTAAATATGGTTA+TGG | 0.309718 | 5.4:+12752180 | MS.gene59402:CDS |
| AAGAATTTGGTGGAAATAAT+GGG | 0.329371 | 5.4:+12752101 | MS.gene59402:CDS |
| ATGGTTATGGGTTCAACCTT+AGG | 0.342083 | 5.4:+12752193 | MS.gene59402:CDS |
| GAAAGCAAGTAGTGATCATA+AGG | 0.388968 | 5.4:+12752246 | MS.gene59402:CDS |
| TGGTTATGGGTTCAACCTTA+GGG | 0.404531 | 5.4:+12752194 | MS.gene59402:CDS |
| ACAAAGGTAAATATGGTTAT+GGG | 0.413220 | 5.4:+12752181 | MS.gene59402:CDS |
| AGGAGCACAAGTGTTCCTTC+AGG | 0.428206 | 5.4:+12752148 | MS.gene59402:CDS |
| CTAATTTCTCATACTTCTCA+TGG | 0.429115 | 5.4:-12752075 | None:intergenic |
| AAGATATGTTGAGTATTTGA+AGG | 0.455536 | 5.4:+12752348 | MS.gene59402:CDS |
| TATGTTGAGTATTTGAAGGA+AGG | 0.471592 | 5.4:+12752352 | MS.gene59402:CDS |
| TTGTAGATTAATATCCCCTA+AGG | 0.515423 | 5.4:-12752209 | None:intergenic |
| GGAGCACAAGTGTTCCTTCA+GGG | 0.516731 | 5.4:+12752149 | MS.gene59402:CDS |
| TTTGTATTTGATGGCCCTGA+AGG | 0.555468 | 5.4:-12752163 | None:intergenic |
| GTTGAGTATTTGAAGGAAGG+AGG | 0.555632 | 5.4:+12752355 | MS.gene59402:CDS |
| TTTGAAGGAAGGAGGCATGT+GGG | 0.559503 | 5.4:+12752363 | MS.gene59402:CDS |
| TATCATTTATAATACATCAC+AGG | 0.563417 | 5.4:-12752403 | None:intergenic |
| AAATTAGATAAAGAATTTGG+TGG | 0.563779 | 5.4:+12752091 | MS.gene59402:CDS |
| ATTTGAAGGAAGGAGGCATG+TGG | 0.565436 | 5.4:+12752362 | MS.gene59402:CDS |
| AAATCCAATAGTACTAGAAG+AGG | 0.567361 | 5.4:+12752052 | MS.gene59402:CDS |
| CAAATGAAGAGTACTTGAAG+AGG | 0.569869 | 5.4:+12752128 | MS.gene59402:CDS |
| AAATCAAAGAAATTGAGAAG+TGG | 0.589565 | 5.4:-12752286 | None:intergenic |
| AGTACTATTGGATTTAAACA+TGG | 0.604551 | 5.4:-12752044 | None:intergenic |
| TTCAGGGCCATCAAATACAA+AGG | 0.612946 | 5.4:+12752165 | MS.gene59402:CDS |
| GGTTATGGGTTCAACCTTAG+GGG | 0.624193 | 5.4:+12752195 | MS.gene59402:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTAGATAAAGAATTTGG+TGG | + | chr5.4:12752091-12752110 | MS.gene59402:CDS | 20.0% |
| !! | ATATTTACCTTTGTATTTGA+TGG | - | chr5.4:12752175-12752194 | None:intergenic | 20.0% |
| !! | ATCAAATACAAAGGTAAATA+TGG | + | chr5.4:12752174-12752193 | MS.gene59402:CDS | 20.0% |
| !! | GAGAAATTAGATAAAGAATT+TGG | + | chr5.4:12752088-12752107 | MS.gene59402:CDS | 20.0% |
| !!! | TTCTCAATTTCTTTGATTTT+CGG | + | chr5.4:12752290-12752309 | MS.gene59402:CDS | 20.0% |
| ! | AAATCAAAGAAATTGAGAAG+TGG | - | chr5.4:12752289-12752308 | None:intergenic | 25.0% |
| ! | AAGATATGTTGAGTATTTGA+AGG | + | chr5.4:12752348-12752367 | MS.gene59402:CDS | 25.0% |
| ! | ACAAAGGTAAATATGGTTAT+GGG | + | chr5.4:12752181-12752200 | MS.gene59402:CDS | 25.0% |
| ! | TACAAAGGTAAATATGGTTA+TGG | + | chr5.4:12752180-12752199 | MS.gene59402:CDS | 25.0% |
| !! | AAAGAATTTGGTGGAAATAA+TGG | + | chr5.4:12752100-12752119 | MS.gene59402:CDS | 25.0% |
| !! | AAGAATTTGGTGGAAATAAT+GGG | + | chr5.4:12752101-12752120 | MS.gene59402:CDS | 25.0% |
| AAATCCAATAGTACTAGAAG+AGG | + | chr5.4:12752052-12752071 | MS.gene59402:CDS | 30.0% | |
| CTAATTTCTCATACTTCTCA+TGG | - | chr5.4:12752078-12752097 | None:intergenic | 30.0% | |
| TATGTTGAGTATTTGAAGGA+AGG | + | chr5.4:12752352-12752371 | MS.gene59402:CDS | 30.0% | |
| TCAACATATCTTGCAAATTC+AGG | - | chr5.4:12752340-12752359 | None:intergenic | 30.0% | |
| TTGTAGATTAATATCCCCTA+AGG | - | chr5.4:12752212-12752231 | None:intergenic | 30.0% | |
| CACTACTTGCTTTCTTTGTT+GGG | - | chr5.4:12752240-12752259 | None:intergenic | 35.0% | |
| GAAAGCAAGTAGTGATCATA+AGG | + | chr5.4:12752246-12752265 | MS.gene59402:CDS | 35.0% | |
| TCACTACTTGCTTTCTTTGT+TGG | - | chr5.4:12752241-12752260 | None:intergenic | 35.0% | |
| !! | CAAATGAAGAGTACTTGAAG+AGG | + | chr5.4:12752128-12752147 | MS.gene59402:CDS | 35.0% |
| ATGGCCTCTTCTAGTACTAT+TGG | - | chr5.4:12752059-12752078 | None:intergenic | 40.0% | |
| ATGGTTATGGGTTCAACCTT+AGG | + | chr5.4:12752193-12752212 | MS.gene59402:CDS | 40.0% | |
| GTTGAGTATTTGAAGGAAGG+AGG | + | chr5.4:12752355-12752374 | MS.gene59402:CDS | 40.0% | |
| TGGTTATGGGTTCAACCTTA+GGG | + | chr5.4:12752194-12752213 | MS.gene59402:CDS | 40.0% | |
| TTCAGGGCCATCAAATACAA+AGG | + | chr5.4:12752165-12752184 | MS.gene59402:CDS | 40.0% | |
| ! | TTTGTATTTGATGGCCCTGA+AGG | - | chr5.4:12752166-12752185 | None:intergenic | 40.0% |
| GGTTATGGGTTCAACCTTAG+GGG | + | chr5.4:12752195-12752214 | MS.gene59402:CDS | 45.0% | |
| ! | ATTTGAAGGAAGGAGGCATG+TGG | + | chr5.4:12752362-12752381 | MS.gene59402:CDS | 45.0% |
| ! | TTTGAAGGAAGGAGGCATGT+GGG | + | chr5.4:12752363-12752382 | MS.gene59402:CDS | 45.0% |
| GGAGCACAAGTGTTCCTTCA+GGG | + | chr5.4:12752149-12752168 | MS.gene59402:CDS | 50.0% | |
| ! | AGGAGCACAAGTGTTCCTTC+AGG | + | chr5.4:12752148-12752167 | MS.gene59402:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 12752046 | 12752423 | 12752046 | ID=MS.gene59402 |
| chr5.4 | mRNA | 12752046 | 12752423 | 12752046 | ID=MS.gene59402.t1;Parent=MS.gene59402 |
| chr5.4 | exon | 12752046 | 12752423 | 12752046 | ID=MS.gene59402.t1.exon1;Parent=MS.gene59402.t1 |
| chr5.4 | CDS | 12752046 | 12752423 | 12752046 | ID=cds.MS.gene59402.t1;Parent=MS.gene59402.t1 |
| Gene Sequence |
| Protein sequence |
