AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene59669

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59669.t1 MTR_5g045560 86.920 237 30 1 1 236 1 237 2.00e-144 403
MS.gene59669.t1 MTR_2g085280 75.847 236 56 1 1 235 1 236 1.86e-131 370
MS.gene59669.t1 MTR_5g055100 76.793 237 54 1 1 236 1 237 4.72e-131 369
MS.gene59669.t1 MTR_2g085250 75.847 236 56 1 1 235 1 236 6.20e-131 369
MS.gene59669.t1 MTR_5g053390 77.333 225 50 1 1 224 1 225 3.84e-125 354
MS.gene59669.t1 MTR_1g075600 72.000 225 62 1 1 224 1 225 2.81e-115 329
MS.gene59669.t1 MTR_8g043650 67.511 237 76 1 1 236 1 237 7.37e-115 328
MS.gene59669.t1 MTR_1g077360 68.444 225 70 1 1 224 1 225 1.11e-108 313
MS.gene59669.t1 MTR_1g105920 68.493 219 68 1 1 218 1 219 5.68e-107 308
MS.gene59669.t1 MTR_2g030740 68.776 237 73 1 1 236 1 237 3.73e-106 306
MS.gene59669.t1 MTR_1g105910 65.333 225 77 1 1 224 1 225 2.28e-104 301
MS.gene59669.t1 MTR_1g012570 67.511 237 76 1 1 236 1 237 3.26e-101 294
MS.gene59669.t1 MTR_3g052920 66.210 219 73 1 1 218 1 219 8.99e-101 292
MS.gene59669.t1 MTR_1g108510 63.404 235 67 4 1 224 1 227 9.58e-98 285
MS.gene59669.t1 MTR_3g030770 63.404 235 67 4 1 224 1 227 2.08e-97 284
MS.gene59669.t1 MTR_0121s0080 59.494 237 94 2 1 236 1 236 2.68e-94 276
MS.gene59669.t1 MTR_1g105905 61.364 220 84 1 18 236 1 220 1.85e-93 273
MS.gene59669.t1 MTR_2g093190 61.702 235 71 4 1 224 1 227 1.02e-92 272
MS.gene59669.t1 MTR_3g052870 62.557 219 62 3 1 218 1 200 2.52e-89 263
MS.gene59669.t1 MTR_1g108580 58.159 239 82 4 1 236 1 224 8.02e-88 259
MS.gene59669.t1 MTR_1g106070 67.582 182 59 0 1 182 1 182 2.92e-86 254
MS.gene59669.t1 MTR_7g062350 51.271 236 112 3 3 236 14 248 1.38e-74 226
MS.gene59669.t1 MTR_1g075570 61.236 178 55 1 18 195 1 164 1.91e-63 195
MS.gene59669.t1 MTR_0121s0100 67.424 132 43 0 1 132 1 132 2.77e-57 178
MS.gene59669.t1 MTR_3g080940 48.864 176 90 0 1 176 1 176 3.12e-57 181
MS.gene59669.t1 MTR_3g030780 62.500 128 48 0 1 128 1 128 2.12e-52 166
MS.gene59669.t1 MTR_3g067910 76.471 102 24 0 21 122 2 103 8.22e-52 164
MS.gene59669.t1 MTR_8g086290 38.788 165 95 2 5 168 8 167 1.15e-32 117
MS.gene59669.t1 MTR_3g067915 61.702 94 36 0 33 126 3 96 3.26e-32 113
MS.gene59669.t1 MTR_2g105290 35.632 174 107 2 5 178 8 176 3.41e-31 113
MS.gene59669.t1 MTR_7g028448 40.645 155 80 2 5 159 8 150 9.66e-30 109
MS.gene59669.t1 MTR_4g036915 40.645 155 80 2 5 159 8 150 9.66e-30 109
MS.gene59669.t1 MTR_3g067875 89.286 56 6 0 18 73 1 56 1.95e-29 105
MS.gene59669.t1 MTR_3g067870 74.603 63 16 0 16 78 83 145 9.53e-25 96.3
MS.gene59669.t1 MTR_4g127140 34.807 181 104 3 5 175 8 184 1.67e-24 96.3
MS.gene59669.t1 MTR_1g108500 54.839 93 21 1 22 114 110 181 6.49e-24 98.2
MS.gene59669.t1 MTR_1g047550 51.948 77 37 0 9 85 2 78 2.55e-23 93.2
MS.gene59669.t1 MTR_8g079502 54.930 71 32 0 9 79 2 72 5.32e-23 92.4
MS.gene59669.t1 MTR_8g051580 43.103 116 62 2 9 122 2 115 7.70e-23 92.0
MS.gene59669.t1 MTR_4g131030 33.908 174 81 4 9 161 2 162 1.14e-22 92.4
MS.gene59669.t1 MTR_1g063160 37.086 151 84 5 9 148 2 152 4.09e-22 89.7
MS.gene59669.t1 MTR_1g054265 48.101 79 41 0 9 87 2 80 4.88e-21 86.7
MS.gene59669.t1 MTR_8g046350 50.649 77 38 0 9 85 2 78 5.98e-21 86.3
MS.gene59669.t1 MTR_4g094632 37.795 127 70 3 9 128 2 126 1.03e-19 83.2
MS.gene59669.t1 MTR_3g005530 52.941 68 32 0 9 76 2 69 8.69e-19 82.0
MS.gene59669.t1 MTR_4g093970 57.576 66 28 0 10 75 29 94 1.51e-18 81.6
MS.gene59669.t1 MTR_4g094638 53.623 69 32 0 9 77 2 70 1.96e-18 80.1
MS.gene59669.t1 MTR_8g033270 51.899 79 37 1 9 86 2 80 1.97e-18 81.6
MS.gene59669.t1 MTR_4g102530 50.685 73 36 0 10 82 3 75 3.06e-18 80.1
MS.gene59669.t1 MTR_3g452380 48.649 74 38 0 3 76 11 84 4.47e-18 80.5
MS.gene59669.t1 MTR_5g066180 50.704 71 35 0 9 79 2 72 7.91e-18 79.7
MS.gene59669.t1 MTR_1g053070 55.882 68 30 0 9 76 2 69 1.43e-17 79.3
MS.gene59669.t1 MTR_4g084740 40.458 131 63 4 9 125 2 131 1.71e-17 80.5
MS.gene59669.t1 MTR_4g109830 52.174 69 32 1 9 76 2 70 3.02e-17 78.2
MS.gene59669.t1 MTR_8g087860 51.471 68 33 0 9 76 18 85 4.87e-17 77.8
MS.gene59669.t1 MTR_7g075870 53.731 67 31 0 10 76 3 69 7.77e-17 76.6
MS.gene59669.t1 MTR_0003s0590 54.412 68 31 0 9 76 2 69 8.91e-17 77.4
MS.gene59669.t1 MTR_2g017865 51.471 68 33 0 9 76 17 84 1.04e-16 77.0
MS.gene59669.t1 MTR_2g009890 46.053 76 41 0 9 84 2 77 1.87e-16 76.6
MS.gene59669.t1 MTR_6g464720 52.174 69 32 1 9 76 2 70 2.39e-16 75.9
MS.gene59669.t1 MTR_1g101970 52.941 68 32 0 9 76 2 69 2.43e-16 74.7
MS.gene59669.t1 MTR_5g032150 50.000 68 34 0 9 76 2 69 2.84e-16 75.5
MS.gene59669.t1 MTR_8g033220 50.725 69 34 0 10 78 3 71 3.38e-16 75.1
MS.gene59669.t1 MTR_8g033220 50.725 69 34 0 10 78 3 71 3.43e-16 75.1
MS.gene59669.t1 MTR_5g032520 50.000 68 34 0 9 76 2 69 3.53e-16 75.1
MS.gene59669.t1 MTR_5g031000 48.529 68 35 0 9 76 2 69 4.19e-16 75.1
MS.gene59669.t1 MTR_5g031000 48.529 68 35 0 9 76 2 69 4.53e-16 75.1
MS.gene59669.t1 MTR_1g029670 34.437 151 75 5 9 157 2 130 7.45e-16 73.2
MS.gene59669.t1 MTR_6g015975 52.174 69 32 1 9 76 2 70 7.57e-16 74.7
MS.gene59669.t1 MTR_7g016600 52.174 69 32 1 9 76 2 70 9.80e-16 74.3
MS.gene59669.t1 MTR_7g075870 52.941 68 31 1 10 76 3 70 1.60e-15 73.2
MS.gene59669.t1 MTR_3g102570 51.429 70 33 1 9 77 2 71 1.67e-15 74.7
MS.gene59669.t1 MTR_5g021270 51.429 70 32 1 9 76 2 71 1.85e-15 73.2
MS.gene59669.t1 MTR_5g021270 51.429 70 32 1 9 76 2 71 1.92e-15 73.2
MS.gene59669.t1 MTR_8g066260 47.826 69 35 1 9 76 2 70 2.14e-15 73.2
MS.gene59669.t1 MTR_7g016630 45.833 72 38 1 9 79 2 73 2.22e-15 73.6
MS.gene59669.t1 MTR_3g088615 47.143 70 35 1 9 76 2 71 2.46e-15 72.0
MS.gene59669.t1 MTR_5g046790 47.826 69 35 1 9 76 2 70 3.57e-15 72.0
MS.gene59669.t1 MTR_2g461710 55.357 56 25 0 9 64 2 57 4.01e-15 68.2
MS.gene59669.t1 MTR_1g038300 51.429 70 32 1 9 76 2 71 4.92e-15 72.0
MS.gene59669.t1 MTR_5g046870 50.725 69 33 1 9 76 2 70 5.41e-15 69.3
MS.gene59669.t1 MTR_8g022970 31.481 162 86 3 10 159 19 167 5.62e-15 70.9
MS.gene59669.t1 MTR_7g075850 47.561 82 40 2 9 87 2 83 6.58e-15 72.0
MS.gene59669.t1 MTR_5g066960 50.877 57 28 0 9 65 2 58 1.38e-14 66.6
MS.gene59669.t1 MTR_3g113030 49.206 63 32 0 9 71 2 64 3.11e-14 69.7
MS.gene59669.t1 MTR_4g109810 55.357 56 25 0 9 64 2 57 6.12e-14 69.3
MS.gene59669.t1 MTR_8g097090 49.275 69 34 1 9 76 2 70 6.31e-14 69.3
MS.gene59669.t1 MTR_3g109930 32.609 138 81 3 27 159 20 150 9.79e-14 67.4
MS.gene59669.t1 MTR_3g437790 30.345 145 80 4 26 159 30 164 1.02e-13 67.8
MS.gene59669.t1 MTR_3g084980 55.357 56 25 0 9 64 2 57 1.02e-13 68.6
MS.gene59669.t1 MTR_4g036050 28.859 149 89 3 9 156 2 134 4.52e-13 65.5
MS.gene59669.t1 MTR_4g036050 28.859 149 89 3 9 156 2 134 4.84e-13 65.9
MS.gene59669.t1 MTR_4g036050 28.859 149 89 3 9 156 2 134 5.75e-13 65.5
MS.gene59669.t1 MTR_4g093030 48.387 62 32 0 9 70 2 63 5.75e-13 65.1
MS.gene59669.t1 MTR_4g036050 28.859 149 89 3 9 156 2 134 6.28e-13 65.9
MS.gene59669.t1 MTR_4g108720 35.833 120 70 4 9 124 2 118 2.98e-12 65.5
MS.gene59669.t1 MTR_4g051538 39.326 89 54 0 1 89 1 89 3.45e-12 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59669.t1 AT5G60440 47.000 200 96 3 5 194 3 202 7.32e-55 178
MS.gene59669.t1 AT2G24840 47.531 162 72 3 9 163 63 218 1.74e-41 142
MS.gene59669.t1 AT1G01530 43.684 190 97 4 4 192 2 182 2.99e-39 136
MS.gene59669.t1 AT1G65360 41.048 229 115 7 5 224 3 220 6.61e-38 132
MS.gene59669.t1 AT4G36590 42.593 162 87 2 5 160 3 164 9.70e-34 122
MS.gene59669.t1 AT3G04100 35.119 168 93 5 2 159 7 168 2.12e-27 104
MS.gene59669.t1 AT1G72350 60.274 73 27 1 9 81 43 113 1.63e-22 92.0
MS.gene59669.t1 AT2G34440 52.113 71 34 0 9 79 2 72 2.24e-21 87.8
MS.gene59669.t1 AT3G66656 54.412 68 31 0 9 76 2 69 1.69e-20 85.5
MS.gene59669.t1 AT1G28460 33.125 160 101 3 5 161 4 160 2.39e-20 85.5
MS.gene59669.t1 AT2G45650 57.353 68 29 0 9 76 2 69 2.01e-19 84.3
MS.gene59669.t1 AT5G51870 55.556 72 32 0 5 76 21 92 4.05e-19 82.4
MS.gene59669.t1 AT2G45660 55.882 68 30 0 10 77 3 70 4.08e-19 81.6
MS.gene59669.t1 AT2G45660 55.882 68 30 0 10 77 3 70 1.05e-18 81.6
MS.gene59669.t1 AT5G51870 35.099 151 66 3 10 128 3 153 1.55e-18 81.3
MS.gene59669.t1 AT4G22950 49.315 73 37 0 10 82 3 75 1.71e-18 80.1
MS.gene59669.t1 AT5G51870 35.065 154 68 3 7 128 10 163 2.39e-18 81.3
MS.gene59669.t1 AT4G09960 51.471 68 33 0 9 76 2 69 2.62e-18 80.9
MS.gene59669.t1 AT5G51870 56.716 67 29 0 10 76 3 69 2.68e-18 79.7
MS.gene59669.t1 AT4G09960 51.471 68 33 0 9 76 3 70 3.13e-18 80.9
MS.gene59669.t1 AT4G09960 51.471 68 33 0 9 76 2 69 3.21e-18 80.9
MS.gene59669.t1 AT5G51870 35.099 151 66 3 10 128 3 153 3.77e-18 80.5
MS.gene59669.t1 AT4G09960 51.471 68 33 0 9 76 12 79 3.88e-18 80.9
MS.gene59669.t1 AT4G09960 51.471 68 33 0 9 76 28 95 6.01e-18 80.5
MS.gene59669.t1 AT4G22950 50.704 71 35 0 10 80 3 73 6.58e-18 79.7
MS.gene59669.t1 AT4G22950 50.704 71 35 0 10 80 3 73 6.58e-18 79.7
MS.gene59669.t1 AT2G22540 33.333 180 100 6 9 185 2 164 7.39e-18 80.1
MS.gene59669.t1 AT2G22540 33.333 180 100 6 9 185 2 164 7.39e-18 80.1
MS.gene59669.t1 AT3G57230 50.000 68 34 0 9 76 2 69 9.01e-18 77.4
MS.gene59669.t1 AT5G62165 53.731 67 31 0 10 76 3 69 1.01e-17 76.3
MS.gene59669.t1 AT1G29962 30.864 162 105 4 5 161 4 163 1.25e-17 78.2
MS.gene59669.t1 AT4G09960 51.471 68 33 0 9 76 97 164 1.48e-17 80.5
MS.gene59669.t1 AT5G62165 53.731 67 31 0 10 76 3 69 1.52e-17 77.4
MS.gene59669.t1 AT5G62165 53.731 67 31 0 10 76 3 69 1.52e-17 77.4
MS.gene59669.t1 AT5G62165 53.731 67 31 0 10 76 3 69 1.52e-17 77.4
MS.gene59669.t1 AT5G62165 53.731 67 31 0 10 76 3 69 2.12e-17 76.6
MS.gene59669.t1 AT5G62165 53.731 67 31 0 10 76 3 69 2.12e-17 76.6
MS.gene59669.t1 AT5G62165 53.731 67 31 0 10 76 3 69 2.12e-17 76.6
MS.gene59669.t1 AT5G62165 53.731 67 31 0 10 76 3 69 2.12e-17 76.6
MS.gene59669.t1 AT5G62165 53.731 67 31 0 10 76 3 69 2.12e-17 76.6
MS.gene59669.t1 AT3G57390 54.412 68 31 0 9 76 2 69 2.32e-17 78.6
MS.gene59669.t1 AT4G37940 51.471 68 33 0 9 76 2 69 3.12e-17 77.8
MS.gene59669.t1 AT4G37940 51.471 68 33 0 9 76 2 69 3.33e-17 77.0
MS.gene59669.t1 AT4G37940 51.471 68 33 0 9 76 2 69 3.38e-17 78.2
MS.gene59669.t1 AT5G10140 50.725 69 33 1 9 76 2 70 3.44e-17 76.6
MS.gene59669.t1 AT3G57230 50.000 68 34 0 9 76 2 69 3.75e-17 78.2
MS.gene59669.t1 AT3G57230 50.000 68 34 0 9 76 2 69 3.75e-17 78.2
MS.gene59669.t1 AT4G18960 49.333 75 38 0 2 76 11 85 3.91e-17 78.2
MS.gene59669.t1 AT5G62165 53.731 67 31 0 10 76 3 69 4.76e-17 77.0
MS.gene59669.t1 AT5G62165 53.731 67 31 0 10 76 3 69 4.76e-17 77.0
MS.gene59669.t1 AT5G10140 50.000 72 35 1 9 79 2 73 4.84e-17 76.6
MS.gene59669.t1 AT3G57390 54.412 68 31 0 9 76 2 69 4.93e-17 78.2
MS.gene59669.t1 AT5G62165 53.731 67 31 0 10 76 3 69 5.10e-17 77.0
MS.gene59669.t1 AT5G62165 53.731 67 31 0 10 76 3 69 5.10e-17 77.0
MS.gene59669.t1 AT5G62165 53.731 67 31 0 10 76 3 69 5.10e-17 77.0
MS.gene59669.t1 AT2G42830 47.368 76 40 0 1 76 9 84 5.31e-17 77.8
MS.gene59669.t1 AT2G42830 47.368 76 40 0 1 76 9 84 5.42e-17 77.8
MS.gene59669.t1 AT5G10140 50.725 69 33 1 9 76 2 70 5.91e-17 76.6
MS.gene59669.t1 AT5G13790 52.941 68 32 0 9 76 2 69 6.31e-17 78.2
MS.gene59669.t1 AT3G58780 50.704 71 35 0 6 76 14 84 6.44e-17 77.8
MS.gene59669.t1 AT5G13790 52.941 68 32 0 9 76 2 69 6.63e-17 77.8
MS.gene59669.t1 AT5G23260 47.727 88 40 2 5 86 14 101 6.91e-17 77.8
MS.gene59669.t1 AT3G58780 50.704 71 35 0 6 76 20 90 7.40e-17 77.4
MS.gene59669.t1 AT5G23260 47.727 88 40 2 5 86 14 101 7.51e-17 77.8
MS.gene59669.t1 AT4G24540 52.941 68 32 0 9 76 2 69 8.04e-17 77.0
MS.gene59669.t1 AT5G10140 50.725 69 33 1 9 76 2 70 8.12e-17 76.3
MS.gene59669.t1 AT1G28450 29.310 174 114 4 5 172 4 174 9.01e-17 75.9
MS.gene59669.t1 AT5G51860 53.731 67 31 0 10 76 3 69 1.23e-16 75.9
MS.gene59669.t1 AT1G77980 45.455 88 47 1 9 95 2 89 1.30e-16 77.8
MS.gene59669.t1 AT3G54340 36.066 122 66 2 9 119 2 122 1.49e-16 76.3
MS.gene59669.t1 AT3G58780 50.704 71 35 0 6 76 14 84 1.61e-16 77.0
MS.gene59669.t1 AT3G30260 31.724 145 69 4 9 123 2 146 1.65e-16 76.6
MS.gene59669.t1 AT5G51860 53.731 67 31 0 10 76 3 69 1.87e-16 75.5
MS.gene59669.t1 AT5G23260 48.810 84 37 2 9 86 2 85 2.71e-16 75.9
MS.gene59669.t1 AT5G23260 48.810 84 37 2 9 86 2 85 2.73e-16 75.9
MS.gene59669.t1 AT3G61120 52.941 68 32 0 9 76 2 69 2.75e-16 75.9
MS.gene59669.t1 AT2G22630 50.000 68 34 0 9 76 2 69 3.08e-16 75.5
MS.gene59669.t1 AT2G22630 50.000 68 34 0 9 76 2 69 3.08e-16 75.5
MS.gene59669.t1 AT1G22130 40.541 111 62 2 9 115 2 112 3.55e-16 76.6
MS.gene59669.t1 AT1G77950 50.000 70 34 1 9 77 2 71 4.45e-16 75.5
MS.gene59669.t1 AT1G77950 50.000 70 34 1 9 77 2 71 4.45e-16 75.5
MS.gene59669.t1 AT1G77950 50.000 70 34 1 9 77 2 71 4.45e-16 75.5
MS.gene59669.t1 AT1G77950 50.000 70 34 1 9 77 2 71 4.45e-16 75.5
MS.gene59669.t1 AT4G11880 50.000 72 35 1 10 80 3 74 8.14e-16 73.2
MS.gene59669.t1 AT4G11880 50.000 72 35 1 10 80 3 74 8.14e-16 73.2
MS.gene59669.t1 AT5G60910 49.275 69 34 1 9 76 2 70 1.03e-15 74.3
MS.gene59669.t1 AT1G26310 49.275 69 34 1 9 76 2 70 1.18e-15 74.3
MS.gene59669.t1 AT4G11880 50.000 72 35 1 10 80 3 74 1.24e-15 73.6
MS.gene59669.t1 AT4G11880 50.000 72 35 1 10 80 3 74 1.24e-15 73.6
MS.gene59669.t1 AT2G14210 49.275 69 34 1 9 76 2 70 1.50e-15 73.6
MS.gene59669.t1 AT5G65050 49.275 69 34 1 9 76 2 70 1.56e-15 72.4
MS.gene59669.t1 AT2G14210 49.275 69 34 1 9 76 2 70 1.88e-15 73.6
MS.gene59669.t1 AT5G15800 52.174 69 32 1 9 76 2 70 2.22e-15 73.6
MS.gene59669.t1 AT2G22540 32.778 180 96 7 9 185 2 159 2.33e-15 73.2
MS.gene59669.t1 AT1G17310 56.757 74 30 1 8 81 47 118 2.36e-15 72.8
MS.gene59669.t1 AT1G17310 56.757 74 30 1 8 81 58 129 3.30e-15 72.8
MS.gene59669.t1 AT5G65050 49.275 69 34 1 9 76 2 70 3.42e-15 72.0
MS.gene59669.t1 AT5G65050 49.275 69 34 1 9 76 2 70 3.50e-15 71.6
MS.gene59669.t1 AT5G15800 52.174 69 32 1 9 76 2 70 3.53e-15 73.2
MS.gene59669.t1 AT1G69120 46.377 69 36 1 9 76 2 70 3.62e-15 72.8
MS.gene59669.t1 AT5G65050 49.275 69 34 1 9 76 2 70 3.71e-15 71.6
MS.gene59669.t1 AT5G20240 48.571 70 34 1 9 76 2 71 5.42e-15 70.5
MS.gene59669.t1 AT1G69120 46.377 69 36 1 9 76 2 70 6.54e-15 72.4
MS.gene59669.t1 AT5G20240 48.571 70 34 1 9 76 2 71 7.32e-15 71.2
MS.gene59669.t1 AT2G03710 51.429 70 32 2 9 76 2 71 9.98e-15 70.5
MS.gene59669.t1 AT5G65060 49.275 69 34 1 9 76 2 70 1.12e-14 70.5
MS.gene59669.t1 AT5G65060 49.275 69 34 1 9 76 2 70 1.18e-14 70.5
MS.gene59669.t1 AT5G65060 49.275 69 34 1 9 76 2 70 1.18e-14 70.5
MS.gene59669.t1 AT1G71692 48.571 70 35 1 9 77 2 71 1.63e-14 70.5
MS.gene59669.t1 AT2G03710 51.429 70 32 2 9 76 2 71 1.99e-14 70.9
MS.gene59669.t1 AT2G03710 51.429 70 32 2 9 76 2 71 2.07e-14 70.9
MS.gene59669.t1 AT5G65070 42.500 80 42 2 9 87 2 78 2.90e-14 68.2
MS.gene59669.t1 AT3G02310 47.826 69 35 1 9 76 2 70 3.42e-14 69.3
MS.gene59669.t1 AT3G58780 52.459 61 29 0 6 66 14 74 3.84e-14 69.7
MS.gene59669.t1 AT1G24260 49.275 69 34 1 9 76 2 70 5.77e-14 69.3
MS.gene59669.t1 AT1G24260 49.275 69 34 1 9 76 2 70 6.23e-14 69.3
MS.gene59669.t1 AT3G58780 52.459 61 29 0 6 66 14 74 6.29e-14 69.7
MS.gene59669.t1 AT1G47760 33.913 115 66 2 9 115 2 114 6.31e-14 68.2
MS.gene59669.t1 AT1G24260 49.275 69 34 1 9 76 2 70 6.43e-14 69.3
MS.gene59669.t1 AT5G65070 44.928 69 37 1 9 76 2 70 6.48e-14 68.6
MS.gene59669.t1 AT3G02310 47.826 69 35 1 9 76 2 70 9.37e-14 68.9
MS.gene59669.t1 AT5G65070 44.928 69 37 1 9 76 2 70 1.55e-13 68.2
MS.gene59669.t1 AT5G65070 42.500 80 42 2 9 87 2 78 1.97e-13 67.4
MS.gene59669.t1 AT5G65080 31.081 148 74 4 1 123 1 145 2.79e-13 66.6
MS.gene59669.t1 AT5G65070 50.877 57 28 0 9 65 2 58 6.19e-13 66.2
MS.gene59669.t1 AT4G37435 36.842 95 55 2 9 98 2 96 1.22e-12 64.7
MS.gene59669.t1 AT1G18750 41.558 77 42 2 9 83 2 77 3.14e-12 65.5
MS.gene59669.t1 AT1G18750 41.558 77 42 2 9 83 2 77 3.34e-12 65.9
MS.gene59669.t1 AT1G18750 41.558 77 42 2 9 83 2 77 3.46e-12 65.5
MS.gene59669.t1 AT2G03060 30.047 213 119 11 9 201 2 204 3.70e-12 65.5
MS.gene59669.t1 AT2G03060 30.047 213 119 11 9 201 2 204 3.70e-12 65.5
MS.gene59669.t1 AT2G03060 30.047 213 119 11 9 201 2 204 3.70e-12 65.5
MS.gene59669.t1 AT2G03060 29.032 217 116 10 9 201 2 204 3.81e-12 65.5
MS.gene59669.t1 AT5G65080 29.655 145 79 3 1 123 1 144 6.62e-12 63.2
MS.gene59669.t1 AT1G46408 36.585 123 68 5 10 128 5 121 7.84e-12 63.9
MS.gene59669.t1 AT5G49420 32.353 136 61 4 5 136 3 111 1.80e-11 63.2
MS.gene59669.t1 AT5G38620 32.331 133 57 4 10 136 6 111 2.21e-11 63.2
MS.gene59669.t1 AT5G49490 40.909 88 45 2 5 85 41 128 2.25e-11 62.8
MS.gene59669.t1 AT2G26320 40.541 74 42 1 5 76 14 87 7.07e-11 58.2

Find 50 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTATTTGGCTTCAATAATA+TGG 0.142453 5.4:+43274628 MS.gene59669:CDS
ATCGCATGATGTCATCATTT+TGG 0.158385 5.4:-43274223 None:intergenic
ATGTTTCAACCTCATTTATT+TGG 0.202774 5.4:+43274614 MS.gene59669:CDS
TCTATGACCGTTTCAACATT+TGG 0.298799 5.4:-43274176 None:intergenic
ATCGAACCTTGTTCAAGATT+TGG 0.319284 5.4:-43274566 None:intergenic
TGAATCATTCAAGAAGGCTT+TGG 0.361264 5.4:+43274421 MS.gene59669:CDS
CTATGACCGTTTCAACATTT+GGG 0.365857 5.4:-43274175 None:intergenic
GGTGGACTTGTCCATTTGAT+AGG 0.370680 5.4:+43274381 MS.gene59669:CDS
TTATTTGGCTTCAATAATAT+GGG 0.386621 5.4:+43274629 MS.gene59669:CDS
CCCCGTTATTCTTTCGCAGT+TGG 0.393777 5.4:-43274349 None:intergenic
ATGCTCCAACGACATGTTAG+AGG 0.400267 5.4:-43274540 None:intergenic
GCCAACTGCGAAAGAATAAC+GGG 0.412211 5.4:+43274348 MS.gene59669:CDS
ATAAATGAGGTTGAAACATT+GGG 0.423985 5.4:-43274610 None:intergenic
AGTGAGCTTTGCACCCTTTG+TGG 0.424656 5.4:+43274095 MS.gene59669:CDS
TAGCAATGAAAGCAACCTTC+AGG 0.435188 5.4:+43274031 MS.gene59669:CDS
ACAAAATTCATCCTATCAAA+TGG 0.436863 5.4:-43274392 None:intergenic
TGCTGATAAGGTTGAAATCC+AGG 0.437169 5.4:+43274472 MS.gene59669:CDS
TGTTGCACATTATGCTGATA+AGG 0.451161 5.4:+43274460 MS.gene59669:CDS
AATAAATGAGGTTGAAACAT+TGG 0.452635 5.4:-43274611 None:intergenic
CCTTCTTGAATGATTCAAGT+TGG 0.473474 5.4:-43274415 None:intergenic
CCAACTTGAATCATTCAAGA+AGG 0.481167 5.4:+43274415 MS.gene59669:CDS
CATAGATCGTTATCTCTCTC+AGG 0.489608 5.4:+43274193 MS.gene59669:CDS
ATCATTCAAGAAGGCTTTGG+AGG 0.496315 5.4:+43274424 MS.gene59669:CDS
AGCCAACTGCGAAAGAATAA+CGG 0.500112 5.4:+43274347 MS.gene59669:CDS
AATAATATGGGAGGAGAAGG+TGG 0.510617 5.4:+43274641 MS.gene59669:CDS
TTCAATAATATGGGAGGAGA+AGG 0.523654 5.4:+43274638 MS.gene59669:CDS
TGGTCACCCAAATGTTGAAA+CGG 0.524927 5.4:+43274169 MS.gene59669:CDS
GTGCTTTAACCATGTCGAGC+GGG 0.525687 5.4:+43273967 None:intergenic
AGCGATATATGCACCACAAA+GGG 0.537976 5.4:-43274108 None:intergenic
GCTCGCGCACACTAGCATTA+CGG 0.539420 5.4:-43274259 None:intergenic
ATGGGAGGAGAAGGTGGGCA+TGG 0.547684 5.4:+43274647 MS.gene59669:CDS
ACTTTCTCAAAGCGCCGTAG+TGG 0.560981 5.4:+43274056 MS.gene59669:CDS
CTTTCTCAAAGCGCCGTAGT+GGG 0.568304 5.4:+43274057 MS.gene59669:CDS
TTCATCCTCTAACATGTCGT+TGG 0.581198 5.4:+43274535 MS.gene59669:CDS
TTATTGAAGCCAAATAAATG+AGG 0.584472 5.4:-43274623 None:intergenic
AATGAAAAGACCTTCTCACT+AGG 0.596511 5.4:-43274146 None:intergenic
TTTGGCTTCAATAATATGGG+AGG 0.603750 5.4:+43274632 MS.gene59669:CDS
TGACATCATGCGATTCATAG+AGG 0.604612 5.4:+43274232 MS.gene59669:CDS
TATATTCTCACCTAGTGAGA+AGG 0.609022 5.4:+43274136 MS.gene59669:CDS
CATCAGCCAAATCTTGAACA+AGG 0.611251 5.4:+43274560 MS.gene59669:CDS
ATAATATGGGAGGAGAAGGT+GGG 0.618375 5.4:+43274642 MS.gene59669:CDS
GCTGATAAGGTTGAAATCCA+GGG 0.621876 5.4:+43274473 MS.gene59669:CDS
CTTTCTTGAAGAGCCCACTA+CGG 0.627906 5.4:-43274070 None:intergenic
GCTTTGAGAAAGTCACCTGA+AGG 0.631860 5.4:-43274046 None:intergenic
GGGGTTGGGTAGAAGTACCC+TGG 0.634945 5.4:-43274490 None:intergenic
GAGCGATATATGCACCACAA+AGG 0.634962 5.4:-43274109 None:intergenic
TCTGATCAAAAGAACTCATG+AGG 0.644620 5.4:-43274671 None:intergenic
GTTGGCTCAGATCATTCGCA+CGG 0.646465 5.4:-43274331 None:intergenic
TGTGCTTTAACCATGTCGAG+CGG 0.682145 5.4:+43273966 None:intergenic
CCAACTGCGAAAGAATAACG+GGG 0.752571 5.4:+43274349 MS.gene59669:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTCAACAAAAAATGGAA+GGG - chr5.4:43274513-43274532 None:intergenic 20.0%
!!! TTATTTGGCTTCAATAATAT+GGG + chr5.4:43274629-43274648 MS.gene59669:CDS 20.0%
!!! TTTATTTGGCTTCAATAATA+TGG + chr5.4:43274628-43274647 MS.gene59669:CDS 20.0%
! AATAAATGAGGTTGAAACAT+TGG - chr5.4:43274614-43274633 None:intergenic 25.0%
! ACAAAATTCATCCTATCAAA+TGG - chr5.4:43274395-43274414 None:intergenic 25.0%
! ATAAATGAGGTTGAAACATT+GGG - chr5.4:43274613-43274632 None:intergenic 25.0%
! ATGTTTCAACCTCATTTATT+TGG + chr5.4:43274614-43274633 MS.gene59669:CDS 25.0%
! TTATTGAAGCCAAATAAATG+AGG - chr5.4:43274626-43274645 None:intergenic 25.0%
!! CATTTTCAACAAAAAATGGA+AGG - chr5.4:43274514-43274533 None:intergenic 25.0%
!! GAACCATTTTCAACAAAAAA+TGG - chr5.4:43274518-43274537 None:intergenic 25.0%
!! TTTTCAACAAAAAATGGAAG+GGG - chr5.4:43274512-43274531 None:intergenic 25.0%
!!! CTTCCATTTTTTGTTGAAAA+TGG + chr5.4:43274512-43274531 MS.gene59669:CDS 25.0%
!!! TCTTTTTCATCTCAATCTTT+TGG - chr5.4:43274010-43274029 None:intergenic 25.0%
GGTTATGTAAAAATTCTGCT+AGG - chr5.4:43274592-43274611 None:intergenic 30.0%
AACAAAAAATGGAAGGGGTT+GGG - chr5.4:43274507-43274526 None:intergenic 35.0%
AATGAAAAGACCTTCTCACT+AGG - chr5.4:43274149-43274168 None:intergenic 35.0%
ATCGAACCTTGTTCAAGATT+TGG - chr5.4:43274569-43274588 None:intergenic 35.0%
CCAACTTGAATCATTCAAGA+AGG + chr5.4:43274415-43274434 MS.gene59669:CDS 35.0%
CCTTCTTGAATGATTCAAGT+TGG - chr5.4:43274418-43274437 None:intergenic 35.0%
CTATGACCGTTTCAACATTT+GGG - chr5.4:43274178-43274197 None:intergenic 35.0%
TATATTCTCACCTAGTGAGA+AGG + chr5.4:43274136-43274155 MS.gene59669:CDS 35.0%
TCTATGACCGTTTCAACATT+TGG - chr5.4:43274179-43274198 None:intergenic 35.0%
TGAATCATTCAAGAAGGCTT+TGG + chr5.4:43274421-43274440 MS.gene59669:CDS 35.0%
TGTTGCACATTATGCTGATA+AGG + chr5.4:43274460-43274479 MS.gene59669:CDS 35.0%
TTCAATAATATGGGAGGAGA+AGG + chr5.4:43274638-43274657 MS.gene59669:CDS 35.0%
! AGTGAGAAGGTCTTTTCATT+TGG + chr5.4:43274149-43274168 MS.gene59669:CDS 35.0%
! ATCGCATGATGTCATCATTT+TGG - chr5.4:43274226-43274245 None:intergenic 35.0%
! TCGCATGATGTCATCATTTT+GGG - chr5.4:43274225-43274244 None:intergenic 35.0%
! TTTGGCTTCAATAATATGGG+AGG + chr5.4:43274632-43274651 MS.gene59669:CDS 35.0%
!! AGAATAACGGGGCTAATTTT+TGG + chr5.4:43274360-43274379 MS.gene59669:CDS 35.0%
AATAATATGGGAGGAGAAGG+TGG + chr5.4:43274641-43274660 MS.gene59669:CDS 40.0%
AGCCAACTGCGAAAGAATAA+CGG + chr5.4:43274347-43274366 MS.gene59669:CDS 40.0%
AGCGATATATGCACCACAAA+GGG - chr5.4:43274111-43274130 None:intergenic 40.0%
ATAATATGGGAGGAGAAGGT+GGG + chr5.4:43274642-43274661 MS.gene59669:CDS 40.0%
CAACAAAAAATGGAAGGGGT+TGG - chr5.4:43274508-43274527 None:intergenic 40.0%
CATAGATCGTTATCTCTCTC+AGG + chr5.4:43274193-43274212 MS.gene59669:CDS 40.0%
CATCAGCCAAATCTTGAACA+AGG + chr5.4:43274560-43274579 MS.gene59669:CDS 40.0%
GCTGATAAGGTTGAAATCCA+GGG + chr5.4:43274473-43274492 MS.gene59669:CDS 40.0%
TAGCAATGAAAGCAACCTTC+AGG + chr5.4:43274031-43274050 MS.gene59669:CDS 40.0%
TGACATCATGCGATTCATAG+AGG + chr5.4:43274232-43274251 MS.gene59669:CDS 40.0%
TGCTGATAAGGTTGAAATCC+AGG + chr5.4:43274472-43274491 MS.gene59669:CDS 40.0%
TGGTCACCCAAATGTTGAAA+CGG + chr5.4:43274169-43274188 MS.gene59669:CDS 40.0%
TTCATCCTCTAACATGTCGT+TGG + chr5.4:43274535-43274554 MS.gene59669:CDS 40.0%
! ATCATTCAAGAAGGCTTTGG+AGG + chr5.4:43274424-43274443 MS.gene59669:CDS 40.0%
! CGCATGATGTCATCATTTTG+GGG - chr5.4:43274224-43274243 None:intergenic 40.0%
!! ATAACGGGGCTAATTTTTGG+TGG + chr5.4:43274363-43274382 MS.gene59669:CDS 40.0%
!! ATGATGTCATCATTTTGGGG+TGG - chr5.4:43274221-43274240 None:intergenic 40.0%
!!! TGATGTCATCATTTTGGGGT+GGG - chr5.4:43274220-43274239 None:intergenic 40.0%
ATGCTCCAACGACATGTTAG+AGG - chr5.4:43274543-43274562 None:intergenic 45.0%
CCAACTGCGAAAGAATAACG+GGG + chr5.4:43274349-43274368 MS.gene59669:CDS 45.0%
CTTTCTTGAAGAGCCCACTA+CGG - chr5.4:43274073-43274092 None:intergenic 45.0%
GAGCGATATATGCACCACAA+AGG - chr5.4:43274112-43274131 None:intergenic 45.0%
GCCAACTGCGAAAGAATAAC+GGG + chr5.4:43274348-43274367 MS.gene59669:CDS 45.0%
TCGAGCGGGAAAAAAACTCA+AGG + chr5.4:43273981-43274000 MS.gene59669:CDS 45.0%
! GGTGGACTTGTCCATTTGAT+AGG + chr5.4:43274381-43274400 MS.gene59669:CDS 45.0%
!! GCTTTGAGAAAGTCACCTGA+AGG - chr5.4:43274049-43274068 None:intergenic 45.0%
AGTGAGCTTTGCACCCTTTG+TGG + chr5.4:43274095-43274114 MS.gene59669:CDS 50.0%
CCCCGTTATTCTTTCGCAGT+TGG - chr5.4:43274352-43274371 None:intergenic 50.0%
! GTTGGCTCAGATCATTCGCA+CGG - chr5.4:43274334-43274353 None:intergenic 50.0%
!! ACTTTCTCAAAGCGCCGTAG+TGG + chr5.4:43274056-43274075 MS.gene59669:CDS 50.0%
!! CTTTCTCAAAGCGCCGTAGT+GGG + chr5.4:43274057-43274076 MS.gene59669:CDS 50.0%
GCTCGCGCACACTAGCATTA+CGG - chr5.4:43274262-43274281 None:intergenic 55.0%
ATGGGAGGAGAAGGTGGGCA+TGG + chr5.4:43274647-43274666 MS.gene59669:CDS 60.0%
!! GGGGTTGGGTAGAAGTACCC+TGG - chr5.4:43274493-43274512 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 43273978 43274688 43273978 ID=MS.gene59669
chr5.4 mRNA 43273978 43274688 43273978 ID=MS.gene59669.t1;Parent=MS.gene59669
chr5.4 exon 43273978 43274688 43273978 ID=MS.gene59669.t1.exon1;Parent=MS.gene59669.t1
chr5.4 CDS 43273978 43274688 43273978 ID=cds.MS.gene59669.t1;Parent=MS.gene59669.t1
Gene Sequence

>MS.gene59669

ATGTCGAGCGGGAAAAAAACTCAAGGTCGCCAAAAGATTGAGATGAAAAAGATTAGCAATGAAAGCAACCTTCAGGTGACTTTCTCAAAGCGCCGTAGTGGGCTCTTCAAGAAAGCTAGTGAGCTTTGCACCCTTTGTGGTGCATATATCGCTCTAATTATATTCTCACCTAGTGAGAAGGTCTTTTCATTTGGTCACCCAAATGTTGAAACGGTCATAGATCGTTATCTCTCTCAGGTCCCACCCCAAAATGATGACATCATGCGATTCATAGAGGCTTACCGTAATGCTAGTGTGCGCGAGCTCAATGATCTATTGACTCGTATGAAAGATGCATTAGACATCAACAAGAACCGTGCGAATGATCTGAGCCAACTGCGAAAGAATAACGGGGCTAATTTTTGGTGGACTTGTCCATTTGATAGGATGAATTTTGTCCAACTTGAATCATTCAAGAAGGCTTTGGAGGATCTTAAAAAACTTGTTGCACATTATGCTGATAAGGTTGAAATCCAGGGTACTTCTACCCAACCCCTTCCATTTTTTGTTGAAAATGGTTCATCCTCTAACATGTCGTTGGAGCATCAGCCAAATCTTGAACAAGGTTCGATCCTAGCAGAATTTTTACATAACCCAATGTTTCAACCTCATTTATTTGGCTTCAATAATATGGGAGGAGAAGGTGGGCATGGTCCTCATGAGTTCTTTTGA
Protein sequence

>MS.gene59669.t1

MSSGKKTQGRQKIEMKKISNESNLQVTFSKRRSGLFKKASELCTLCGAYIALIIFSPSEKVFSFGHPNVETVIDRYLSQVPPQNDDIMRFIEAYRNASVRELNDLLTRMKDALDINKNRANDLSQLRKNNGANFWWTCPFDRMNFVQLESFKKALEDLKKLVAHYADKVEIQGTSTQPLPFFVENGSSSNMSLEHQPNLEQGSILAEFLHNPMFQPHLFGFNNMGGEGGHGPHEFF