Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59675.t1 | XP_003614502.1 | 99.2 | 120 | 1 | 0 | 1 | 120 | 37 | 156 | 9.00E-61 | 242.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59675.t1 | Q9SX29 | 84.8 | 112 | 17 | 0 | 9 | 120 | 48 | 159 | 6.5e-52 | 204.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59675.t1 | G7JYE1 | 99.2 | 120 | 1 | 0 | 1 | 120 | 37 | 156 | 6.5e-61 | 242.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053895 | MS.gene59675 | 0.82185 | 3.24E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59675.t1 | MTR_5g054770 | 99.167 | 120 | 1 | 0 | 1 | 120 | 37 | 156 | 3.73e-86 | 248 |
| MS.gene59675.t1 | MTR_6g085050 | 82.609 | 115 | 19 | 1 | 7 | 120 | 46 | 160 | 5.89e-69 | 204 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59675.t1 | AT1G68660 | 84.821 | 112 | 17 | 0 | 9 | 120 | 48 | 159 | 3.13e-71 | 210 |
| MS.gene59675.t1 | AT1G68660 | 62.500 | 112 | 12 | 1 | 9 | 120 | 48 | 129 | 1.97e-44 | 141 |
Find 40 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGGTGGCTTTCTCAATAAC+TGG | 0.289306 | 5.4:-43326338 | None:intergenic |
| TCAAATCAAATTCAGATTCC+CGG | 0.395114 | 5.4:-43326364 | None:intergenic |
| GATCATTGTATGCAACTTAG+AGG | 0.399829 | 5.4:+43327104 | MS.gene59675:CDS |
| TTGTGCAATATTACACGATA+TGG | 0.414798 | 5.4:-43326933 | None:intergenic |
| GCATTATCAAGGGTCATTCC+AGG | 0.425052 | 5.4:-43327008 | None:intergenic |
| TCAAATTCAGATTCCCGGCC+TGG | 0.431777 | 5.4:-43326359 | None:intergenic |
| TAACAAAGGGATCTGGTAAA+GGG | 0.446713 | 5.4:+43326297 | MS.gene59675:CDS |
| TGATGTTAACTGCATTATCA+AGG | 0.464225 | 5.4:-43327019 | None:intergenic |
| CAAATTCAGATTCCCGGCCT+GGG | 0.480296 | 5.4:-43326358 | None:intergenic |
| TTAACAAAGGGATCTGGTAA+AGG | 0.483230 | 5.4:+43326296 | MS.gene59675:CDS |
| TGAGAAAGCCACCCCAGGCC+GGG | 0.496954 | 5.4:+43326346 | MS.gene59675:CDS |
| GCTGTATTAACAAAGGGATC+TGG | 0.501137 | 5.4:+43326290 | MS.gene59675:CDS |
| CCCTTTGTTAATACAGCCAT+GGG | 0.501164 | 5.4:-43326284 | None:intergenic |
| TGTATGCAACTTAGAGGCAA+TGG | 0.502603 | 5.4:+43327110 | MS.gene59675:CDS |
| CTCAGTTCAATTGAACCTGC+AGG | 0.507343 | 5.4:+43327137 | MS.gene59675:CDS |
| AATGATCTTCAGCATCAGCT+TGG | 0.508043 | 5.4:-43327088 | None:intergenic |
| ATGATCTTCAGCATCAGCTT+GGG | 0.510156 | 5.4:-43327087 | None:intergenic |
| TTCAGATTCCCGGCCTGGGG+TGG | 0.510475 | 5.4:-43326354 | None:intergenic |
| CCCATGGCTGTATTAACAAA+GGG | 0.520316 | 5.4:+43326284 | None:intergenic |
| AACAAAGGGATCTGGTAAAG+GGG | 0.524640 | 5.4:+43326298 | MS.gene59675:CDS |
| TCCCTTTGTTAATACAGCCA+TGG | 0.529399 | 5.4:-43326285 | None:intergenic |
| TTGAGAAAGCCACCCCAGGC+CGG | 0.534808 | 5.4:+43326345 | MS.gene59675:CDS |
| GGATCACCTTCATAAGTACT+TGG | 0.538715 | 5.4:-43326986 | None:intergenic |
| CTAAGATGTGCAGGAAATCA+CGG | 0.554342 | 5.4:+43326898 | MS.gene59675:intron |
| CATCAGCAACCATCACCTGC+AGG | 0.555211 | 5.4:-43327152 | None:intergenic |
| TGTTGTCCAAGTACTTATGA+AGG | 0.564357 | 5.4:+43326980 | MS.gene59675:CDS |
| GTACTTATGAAGGTGATCCC+TGG | 0.569299 | 5.4:+43326990 | MS.gene59675:CDS |
| GTTATTGAGAAAGCCACCCC+AGG | 0.591201 | 5.4:+43326341 | MS.gene59675:CDS |
| TCAATTGAACCTGCAGGTGA+TGG | 0.594938 | 5.4:+43327143 | MS.gene59675:CDS |
| TCCCATGGCTGTATTAACAA+AGG | 0.597866 | 5.4:+43326283 | None:intergenic |
| CATTATCAAGGGTCATTCCA+GGG | 0.602128 | 5.4:-43327007 | None:intergenic |
| GATGTTAACTGCATTATCAA+GGG | 0.610327 | 5.4:-43327018 | None:intergenic |
| CAATGACAACTTCAACAGAC+GGG | 0.612734 | 5.4:+43326953 | MS.gene59675:CDS |
| TGCAATATTACACGATATGG+CGG | 0.626220 | 5.4:-43326930 | None:intergenic |
| ACAATGACAACTTCAACAGA+CGG | 0.629056 | 5.4:+43326952 | MS.gene59675:CDS |
| ATGCAAGAAGCTCACCACAA+TGG | 0.636426 | 5.4:+43327041 | MS.gene59675:CDS |
| AAATTCAGATTCCCGGCCTG+GGG | 0.643776 | 5.4:-43326357 | None:intergenic |
| TCACCACAATGGAATGTCAG+TGG | 0.665125 | 5.4:+43327052 | MS.gene59675:CDS |
| ACAAAGGGATCTGGTAAAGG+GGG | 0.679983 | 5.4:+43326299 | MS.gene59675:CDS |
| TCACCACTGACATTCCATTG+TGG | 0.707546 | 5.4:-43327055 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATTTATGATGATTTTCAA+TGG | + | chr5.4:43326549-43326568 | MS.gene59675:intron | 15.0% |
| !!! | AATTTATGATGATTTTCAAT+GGG | + | chr5.4:43326550-43326569 | MS.gene59675:intron | 15.0% |
| !! | AGTCATAAAACTTAAACAAT+CGG | - | chr5.4:43326826-43326845 | None:intergenic | 20.0% |
| !! | AGTCATTTAGAAAATATGAT+AGG | + | chr5.4:43326719-43326738 | MS.gene59675:intron | 20.0% |
| !!! | AAAAAGAAAGTATTTGATTC+TGG | + | chr5.4:43326870-43326889 | MS.gene59675:intron | 20.0% |
| !!! | TTGTTTTTCTTAATGTGATT+AGG | + | chr5.4:43326448-43326467 | MS.gene59675:intron | 20.0% |
| !! | ACAAGTAATACTTGATGTTT+TGG | + | chr5.4:43326635-43326654 | MS.gene59675:intron | 25.0% |
| !! | TTATTAGTGATGATAACGTA+AGG | + | chr5.4:43326579-43326598 | MS.gene59675:intron | 25.0% |
| !!! | CATCAAGTATTACTTGTTTT+AGG | - | chr5.4:43326632-43326651 | None:intergenic | 25.0% |
| ACGTTATCATCACTAATAAC+TGG | - | chr5.4:43326578-43326597 | None:intergenic | 30.0% | |
| CAAGTATCTTCAAATGTCAA+TGG | - | chr5.4:43326514-43326533 | None:intergenic | 30.0% | |
| CTTAACGATGAAATTGAAGT+TGG | + | chr5.4:43326758-43326777 | MS.gene59675:intron | 30.0% | |
| GATGTTAACTGCATTATCAA+GGG | - | chr5.4:43327021-43327040 | None:intergenic | 30.0% | |
| TCAAATCAAATTCAGATTCC+CGG | - | chr5.4:43326367-43326386 | None:intergenic | 30.0% | |
| TGATGTTAACTGCATTATCA+AGG | - | chr5.4:43327022-43327041 | None:intergenic | 30.0% | |
| TTGTGCAATATTACACGATA+TGG | - | chr5.4:43326936-43326955 | None:intergenic | 30.0% | |
| !! | AGATACTTGTTATTGTGCAA+TGG | + | chr5.4:43326525-43326544 | MS.gene59675:intron | 30.0% |
| !! | GATACTTGTTATTGTGCAAT+GGG | + | chr5.4:43326526-43326545 | MS.gene59675:intron | 30.0% |
| ACAATGACAACTTCAACAGA+CGG | + | chr5.4:43326952-43326971 | MS.gene59675:CDS | 35.0% | |
| ATGTCATCGCATGTAGTAAA+AGG | + | chr5.4:43326603-43326622 | MS.gene59675:intron | 35.0% | |
| GATCATTGTATGCAACTTAG+AGG | + | chr5.4:43327104-43327123 | MS.gene59675:CDS | 35.0% | |
| TAACAAAGGGATCTGGTAAA+GGG | + | chr5.4:43326297-43326316 | MS.gene59675:CDS | 35.0% | |
| TATGATAGGATGTTTAAGCG+TGG | + | chr5.4:43326733-43326752 | MS.gene59675:intron | 35.0% | |
| TGCAATATTACACGATATGG+CGG | - | chr5.4:43326933-43326952 | None:intergenic | 35.0% | |
| TGTTGTCCAAGTACTTATGA+AGG | + | chr5.4:43326980-43326999 | MS.gene59675:CDS | 35.0% | |
| TTAACAAAGGGATCTGGTAA+AGG | + | chr5.4:43326296-43326315 | MS.gene59675:CDS | 35.0% | |
| AACAAAGGGATCTGGTAAAG+GGG | + | chr5.4:43326298-43326317 | MS.gene59675:CDS | 40.0% | |
| AATGATCTTCAGCATCAGCT+TGG | - | chr5.4:43327091-43327110 | None:intergenic | 40.0% | |
| ATGATCTTCAGCATCAGCTT+GGG | - | chr5.4:43327090-43327109 | None:intergenic | 40.0% | |
| CAATGACAACTTCAACAGAC+GGG | + | chr5.4:43326953-43326972 | MS.gene59675:CDS | 40.0% | |
| CATTATCAAGGGTCATTCCA+GGG | - | chr5.4:43327010-43327029 | None:intergenic | 40.0% | |
| CTAAGATGTGCAGGAAATCA+CGG | + | chr5.4:43326898-43326917 | MS.gene59675:intron | 40.0% | |
| GCTGTATTAACAAAGGGATC+TGG | + | chr5.4:43326290-43326309 | MS.gene59675:CDS | 40.0% | |
| GGATCACCTTCATAAGTACT+TGG | - | chr5.4:43326989-43327008 | None:intergenic | 40.0% | |
| TGTATGCAACTTAGAGGCAA+TGG | + | chr5.4:43327110-43327129 | MS.gene59675:CDS | 40.0% | |
| !!! | TCTCAATAACTGGCTTTTCC+AGG | - | chr5.4:43326331-43326350 | None:intergenic | 40.0% |
| ACAAAGGGATCTGGTAAAGG+GGG | + | chr5.4:43326299-43326318 | MS.gene59675:CDS | 45.0% | |
| ATCGGCACAACAGAACAACA+CGG | - | chr5.4:43326808-43326827 | None:intergenic | 45.0% | |
| ATGCAAGAAGCTCACCACAA+TGG | + | chr5.4:43327041-43327060 | MS.gene59675:CDS | 45.0% | |
| CGGCATCTAAATCACTACAG+TGG | - | chr5.4:43326788-43326807 | None:intergenic | 45.0% | |
| CTCAGTTCAATTGAACCTGC+AGG | + | chr5.4:43327137-43327156 | MS.gene59675:CDS | 45.0% | |
| GCATTATCAAGGGTCATTCC+AGG | - | chr5.4:43327011-43327030 | None:intergenic | 45.0% | |
| GTACTTATGAAGGTGATCCC+TGG | + | chr5.4:43326990-43327009 | MS.gene59675:CDS | 45.0% | |
| TCAATTGAACCTGCAGGTGA+TGG | + | chr5.4:43327143-43327162 | MS.gene59675:CDS | 45.0% | |
| TCACCACAATGGAATGTCAG+TGG | + | chr5.4:43327052-43327071 | MS.gene59675:CDS | 45.0% | |
| TCACCACTGACATTCCATTG+TGG | - | chr5.4:43327058-43327077 | None:intergenic | 45.0% | |
| !! | CTGGCATTTCTAAGATGTGC+AGG | + | chr5.4:43326889-43326908 | MS.gene59675:intron | 45.0% |
| AAATTCAGATTCCCGGCCTG+GGG | - | chr5.4:43326360-43326379 | None:intergenic | 50.0% | |
| CAAATTCAGATTCCCGGCCT+GGG | - | chr5.4:43326361-43326380 | None:intergenic | 50.0% | |
| GGGGTGGCTTTCTCAATAAC+TGG | - | chr5.4:43326341-43326360 | None:intergenic | 50.0% | |
| GTTATTGAGAAAGCCACCCC+AGG | + | chr5.4:43326341-43326360 | MS.gene59675:CDS | 50.0% | |
| TCAAATTCAGATTCCCGGCC+TGG | - | chr5.4:43326362-43326381 | None:intergenic | 50.0% | |
| AAGGGATCTGGTAAAGGGGG+AGG | + | chr5.4:43326302-43326321 | MS.gene59675:CDS | 55.0% | |
| TGGTAAAGGGGGAGGAATCC+TGG | + | chr5.4:43326310-43326329 | MS.gene59675:CDS | 55.0% | |
| TTGAGAAAGCCACCCCAGGC+CGG | + | chr5.4:43326345-43326364 | MS.gene59675:CDS | 60.0% | |
| TGAGAAAGCCACCCCAGGCC+GGG | + | chr5.4:43326346-43326365 | MS.gene59675:CDS | 65.0% | |
| TTCAGATTCCCGGCCTGGGG+TGG | - | chr5.4:43326357-43326376 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 43326287 | 43327172 | 43326287 | ID=MS.gene59675 |
| chr5.4 | mRNA | 43326287 | 43327172 | 43326287 | ID=MS.gene59675.t1;Parent=MS.gene59675 |
| chr5.4 | exon | 43326287 | 43326387 | 43326287 | ID=MS.gene59675.t1.exon1;Parent=MS.gene59675.t1 |
| chr5.4 | CDS | 43326287 | 43326387 | 43326287 | ID=cds.MS.gene59675.t1;Parent=MS.gene59675.t1 |
| chr5.4 | exon | 43326911 | 43327172 | 43326911 | ID=MS.gene59675.t1.exon2;Parent=MS.gene59675.t1 |
| chr5.4 | CDS | 43326911 | 43327172 | 43326911 | ID=cds.MS.gene59675.t1;Parent=MS.gene59675.t1 |
| Gene Sequence |
| Protein sequence |
