Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053895.t1 | XP_003614502.1 | 98.3 | 121 | 1 | 1 | 1 | 121 | 37 | 156 | 2.20E-59 | 238 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053895.t1 | Q9SX29 | 78.5 | 121 | 26 | 0 | 1 | 121 | 39 | 159 | 9.5e-51 | 200.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053895.t1 | G7JYE1 | 98.3 | 121 | 1 | 1 | 1 | 121 | 37 | 156 | 1.6e-59 | 238.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053786 | MS.gene053895 | -0.816347 | 5.80E-52 | -1.69E-46 |
| MS.gene053895 | MS.gene056403 | -0.801254 | 9.82E-49 | -1.69E-46 |
| MS.gene053895 | MS.gene061898 | -0.849647 | 2.83E-60 | -1.69E-46 |
| MS.gene053895 | MS.gene069225 | -0.815096 | 1.10E-51 | -1.69E-46 |
| MS.gene053895 | MS.gene06998 | -0.801934 | 7.12E-49 | -1.69E-46 |
| MS.gene053895 | MS.gene070260 | -0.804981 | 1.66E-49 | -1.69E-46 |
| MS.gene053895 | MS.gene072328 | -0.810438 | 1.15E-50 | -1.69E-46 |
| MS.gene053895 | MS.gene072329 | -0.809288 | 2.04E-50 | -1.69E-46 |
| MS.gene053895 | MS.gene072762 | -0.821435 | 4.04E-53 | -1.69E-46 |
| MS.gene053895 | MS.gene073289 | -0.802827 | 4.66E-49 | -1.69E-46 |
| MS.gene053895 | MS.gene09852 | -0.813124 | 3.00E-51 | -1.69E-46 |
| MS.gene053895 | MS.gene21680 | -0.81871 | 1.70E-52 | -1.69E-46 |
| MS.gene053895 | MS.gene26396 | -0.829282 | 5.62E-55 | -1.69E-46 |
| MS.gene053895 | MS.gene26426 | -0.825045 | 5.81E-54 | -1.69E-46 |
| MS.gene053895 | MS.gene26605 | -0.804364 | 2.24E-49 | -1.69E-46 |
| MS.gene053895 | MS.gene29719 | -0.829256 | 5.70E-55 | -1.69E-46 |
| MS.gene053895 | MS.gene38070 | -0.805176 | 1.51E-49 | -1.69E-46 |
| MS.gene053895 | MS.gene38262 | -0.806977 | 6.33E-50 | -1.69E-46 |
| MS.gene053895 | MS.gene45785 | -0.830195 | 3.37E-55 | -1.69E-46 |
| MS.gene053895 | MS.gene47554 | -0.823158 | 1.61E-53 | -1.69E-46 |
| MS.gene053895 | MS.gene47581 | -0.8181 | 2.34E-52 | -1.69E-46 |
| MS.gene053895 | MS.gene47985 | -0.813049 | 3.12E-51 | -1.69E-46 |
| MS.gene053895 | MS.gene51111 | -0.803683 | 3.10E-49 | -1.69E-46 |
| MS.gene053895 | MS.gene57325 | -0.807871 | 4.09E-50 | -1.69E-46 |
| MS.gene053895 | MS.gene59675 | 0.82185 | 3.24E-53 | -1.69E-46 |
| MS.gene053895 | MS.gene73825 | -0.803262 | 3.79E-49 | -1.69E-46 |
| MS.gene053895 | MS.gene75564 | -0.805758 | 1.14E-49 | -1.69E-46 |
| MS.gene053895 | MS.gene81288 | -0.807291 | 5.43E-50 | -1.69E-46 |
| MS.gene053895 | MS.gene85610 | -0.810002 | 1.43E-50 | -1.69E-46 |
| MS.gene053895 | MS.gene88231 | -0.803071 | 4.15E-49 | -1.69E-46 |
| MS.gene053895 | MS.gene90712 | 0.811101 | 8.28E-51 | -1.69E-46 |
| MS.gene053895 | MS.gene94183 | -0.803191 | 3.92E-49 | -1.69E-46 |
| MS.gene053895 | MS.gene95049 | -0.81193 | 5.47E-51 | -1.69E-46 |
| MS.gene053895 | MS.gene96527 | 0.943182 | 1.91E-102 | -1.69E-46 |
| MS.gene053895 | MS.gene98889 | -0.808927 | 2.44E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053895.t1 | MTR_5g054770 | 98.347 | 121 | 1 | 1 | 1 | 121 | 37 | 156 | 2.74e-84 | 243 |
| MS.gene053895.t1 | MTR_6g085050 | 80.000 | 115 | 23 | 0 | 7 | 121 | 46 | 160 | 1.70e-67 | 201 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053895.t1 | AT1G68660 | 78.512 | 121 | 26 | 0 | 1 | 121 | 39 | 159 | 4.94e-70 | 207 |
| MS.gene053895.t1 | AT1G68660 | 57.851 | 121 | 21 | 1 | 1 | 121 | 39 | 129 | 4.07e-43 | 138 |
Find 37 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGGTGGCTTTCTCAATAAC+TGG | 0.289306 | 5.3:-43430782 | None:intergenic |
| TCAAATCAAATTCAGATTCC+CGG | 0.395114 | 5.3:-43430808 | None:intergenic |
| GATCATTGTATGCAACTTAG+AGG | 0.399829 | 5.3:+43431550 | MS.gene053895:CDS |
| TTGTGCAATATTACACGATA+TGG | 0.414798 | 5.3:-43431379 | None:intergenic |
| TAACAAAAGGATCTGGTAAA+GGG | 0.423712 | 5.3:+43430738 | MS.gene053895:CDS |
| GCATTATCAAGGGTCATTCC+AGG | 0.425052 | 5.3:-43431454 | None:intergenic |
| TCAAATTCAGATTCCCGGCC+TGG | 0.431777 | 5.3:-43430803 | None:intergenic |
| TGATGTTAACTGCATTATCA+AGG | 0.464225 | 5.3:-43431465 | None:intergenic |
| CCCATGGCTGTATTAACAAA+AGG | 0.470418 | 5.3:+43430725 | None:intergenic |
| CAAATTCAGATTCCCGGCCT+GGG | 0.480296 | 5.3:-43430802 | None:intergenic |
| GCTGTATTAACAAAAGGATC+TGG | 0.483670 | 5.3:+43430731 | MS.gene053895:CDS |
| TTAACAAAAGGATCTGGTAA+AGG | 0.488662 | 5.3:+43430737 | MS.gene053895:CDS |
| TGAGAAAGCCACCCCAGGCC+GGG | 0.496954 | 5.3:+43430790 | MS.gene053895:CDS |
| TGTATGCAACTTAGAGGCAA+TGG | 0.502603 | 5.3:+43431556 | MS.gene053895:CDS |
| CTCAGTTCAATTGAACCTGC+AGG | 0.507343 | 5.3:+43431583 | MS.gene053895:CDS |
| AATGATCTTCAGCATCAGCT+TGG | 0.508043 | 5.3:-43431534 | None:intergenic |
| ATGATCTTCAGCATCAGCTT+GGG | 0.510156 | 5.3:-43431533 | None:intergenic |
| TTCAGATTCCCGGCCTGGGG+TGG | 0.510475 | 5.3:-43430798 | None:intergenic |
| AACAAAAGGATCTGGTAAAG+GGG | 0.514249 | 5.3:+43430739 | MS.gene053895:CDS |
| TTGAGAAAGCCACCCCAGGC+CGG | 0.534808 | 5.3:+43430789 | MS.gene053895:CDS |
| GGATCACCTTCATAAGTACT+TGG | 0.538715 | 5.3:-43431432 | None:intergenic |
| CTAAGATGTGCAGGAAATCA+CGG | 0.554342 | 5.3:+43431344 | MS.gene053895:intron |
| CATCAGCAACCATCACCTGC+AGG | 0.555211 | 5.3:-43431598 | None:intergenic |
| TGTTGTCCAAGTACTTATGA+AGG | 0.564357 | 5.3:+43431426 | MS.gene053895:CDS |
| GTACTTATGAAGGTGATCCC+TGG | 0.569299 | 5.3:+43431436 | MS.gene053895:CDS |
| GTTATTGAGAAAGCCACCCC+AGG | 0.591201 | 5.3:+43430785 | MS.gene053895:CDS |
| TCAATTGAACCTGCAGGTGA+TGG | 0.594938 | 5.3:+43431589 | MS.gene053895:CDS |
| CATTATCAAGGGTCATTCCA+GGG | 0.602128 | 5.3:-43431453 | None:intergenic |
| GATGTTAACTGCATTATCAA+GGG | 0.610327 | 5.3:-43431464 | None:intergenic |
| CAATGACAACTTCAACAGAC+GGG | 0.612734 | 5.3:+43431399 | MS.gene053895:CDS |
| TGCAATATTACACGATATGG+CGG | 0.626220 | 5.3:-43431376 | None:intergenic |
| ACAATGACAACTTCAACAGA+CGG | 0.629056 | 5.3:+43431398 | MS.gene053895:CDS |
| ATGCAAGAAGCTCACCACAA+TGG | 0.636426 | 5.3:+43431487 | MS.gene053895:CDS |
| AAATTCAGATTCCCGGCCTG+GGG | 0.643776 | 5.3:-43430801 | None:intergenic |
| ACAAAAGGATCTGGTAAAGG+GGG | 0.662590 | 5.3:+43430740 | MS.gene053895:CDS |
| TCACCACAATGGAATGTCAG+TGG | 0.665125 | 5.3:+43431498 | MS.gene053895:CDS |
| TCACCACTGACATTCCATTG+TGG | 0.707546 | 5.3:-43431501 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATTTATGATGATTTTCAA+TGG | + | chr5.3:43430993-43431012 | MS.gene053895:intron | 15.0% |
| !!! | AATTTATGATGATTTTCAAT+GGG | + | chr5.3:43430994-43431013 | MS.gene053895:intron | 15.0% |
| !! | AGTCATTTAGAAAATATGAT+AGG | + | chr5.3:43431165-43431184 | MS.gene053895:intron | 20.0% |
| !! | TTATTAGTGATGATAACTTA+AGG | + | chr5.3:43431023-43431042 | MS.gene053895:intron | 20.0% |
| !!! | AAAAAGAAAGTATTTGATTC+TGG | + | chr5.3:43431316-43431335 | MS.gene053895:intron | 20.0% |
| ! | AAGTTATCATCACTAATAAC+TGG | - | chr5.3:43431022-43431041 | None:intergenic | 25.0% |
| !! | ACAAGTAATACTTGATGTTT+TGG | + | chr5.3:43431079-43431098 | MS.gene053895:intron | 25.0% |
| !!! | CATCAAGTATTACTTGTTTT+AGG | - | chr5.3:43431076-43431095 | None:intergenic | 25.0% |
| !!! | TTGTTTTTCTGAATGTGATT+AGG | + | chr5.3:43430892-43430911 | MS.gene053895:intron | 25.0% |
| CAAGTATCTTCAAATGTCAA+TGG | - | chr5.3:43430958-43430977 | None:intergenic | 30.0% | |
| CTTAACGATGAAATTGAAGT+TGG | + | chr5.3:43431204-43431223 | MS.gene053895:intron | 30.0% | |
| GATGTTAACTGCATTATCAA+GGG | - | chr5.3:43431467-43431486 | None:intergenic | 30.0% | |
| TAACAAAAGGATCTGGTAAA+GGG | + | chr5.3:43430738-43430757 | MS.gene053895:CDS | 30.0% | |
| TCAAATCAAATTCAGATTCC+CGG | - | chr5.3:43430811-43430830 | None:intergenic | 30.0% | |
| TGATGTTAACTGCATTATCA+AGG | - | chr5.3:43431468-43431487 | None:intergenic | 30.0% | |
| TTAACAAAAGGATCTGGTAA+AGG | + | chr5.3:43430737-43430756 | MS.gene053895:CDS | 30.0% | |
| TTGTGCAATATTACACGATA+TGG | - | chr5.3:43431382-43431401 | None:intergenic | 30.0% | |
| !! | AGATACTTGTTATTGTGCAA+TGG | + | chr5.3:43430969-43430988 | MS.gene053895:intron | 30.0% |
| !! | GATACTTGTTATTGTGCAAT+GGG | + | chr5.3:43430970-43430989 | MS.gene053895:intron | 30.0% |
| AACAAAAGGATCTGGTAAAG+GGG | + | chr5.3:43430739-43430758 | MS.gene053895:CDS | 35.0% | |
| ACAATGACAACTTCAACAGA+CGG | + | chr5.3:43431398-43431417 | MS.gene053895:CDS | 35.0% | |
| ATGTCATCGCATGTAGTAAA+AGG | + | chr5.3:43431047-43431066 | MS.gene053895:intron | 35.0% | |
| GATCATTGTATGCAACTTAG+AGG | + | chr5.3:43431550-43431569 | MS.gene053895:CDS | 35.0% | |
| GCTGTATTAACAAAAGGATC+TGG | + | chr5.3:43430731-43430750 | MS.gene053895:CDS | 35.0% | |
| TATGATAGGATGTTTAAGCG+TGG | + | chr5.3:43431179-43431198 | MS.gene053895:intron | 35.0% | |
| TGCAATATTACACGATATGG+CGG | - | chr5.3:43431379-43431398 | None:intergenic | 35.0% | |
| TGTTGTCCAAGTACTTATGA+AGG | + | chr5.3:43431426-43431445 | MS.gene053895:CDS | 35.0% | |
| AATGATCTTCAGCATCAGCT+TGG | - | chr5.3:43431537-43431556 | None:intergenic | 40.0% | |
| ACAAAAGGATCTGGTAAAGG+GGG | + | chr5.3:43430740-43430759 | MS.gene053895:CDS | 40.0% | |
| ATGATCTTCAGCATCAGCTT+GGG | - | chr5.3:43431536-43431555 | None:intergenic | 40.0% | |
| CAATGACAACTTCAACAGAC+GGG | + | chr5.3:43431399-43431418 | MS.gene053895:CDS | 40.0% | |
| CATTATCAAGGGTCATTCCA+GGG | - | chr5.3:43431456-43431475 | None:intergenic | 40.0% | |
| CGACAACTAAATCACTACAG+TGG | - | chr5.3:43431234-43431253 | None:intergenic | 40.0% | |
| CTAAGATGTGCAGGAAATCA+CGG | + | chr5.3:43431344-43431363 | MS.gene053895:intron | 40.0% | |
| GGATCACCTTCATAAGTACT+TGG | - | chr5.3:43431435-43431454 | None:intergenic | 40.0% | |
| TGTATGCAACTTAGAGGCAA+TGG | + | chr5.3:43431556-43431575 | MS.gene053895:CDS | 40.0% | |
| ! | TGTTGTTCTGTTGTGCTGAT+TGG | + | chr5.3:43431254-43431273 | MS.gene053895:intron | 40.0% |
| !!! | TCTCAATAACTGGCTTTTCC+AGG | - | chr5.3:43430775-43430794 | None:intergenic | 40.0% |
| ATGCAAGAAGCTCACCACAA+TGG | + | chr5.3:43431487-43431506 | MS.gene053895:CDS | 45.0% | |
| CTCAGTTCAATTGAACCTGC+AGG | + | chr5.3:43431583-43431602 | MS.gene053895:CDS | 45.0% | |
| GCATTATCAAGGGTCATTCC+AGG | - | chr5.3:43431457-43431476 | None:intergenic | 45.0% | |
| GTACTTATGAAGGTGATCCC+TGG | + | chr5.3:43431436-43431455 | MS.gene053895:CDS | 45.0% | |
| TCAATTGAACCTGCAGGTGA+TGG | + | chr5.3:43431589-43431608 | MS.gene053895:CDS | 45.0% | |
| TCACCACAATGGAATGTCAG+TGG | + | chr5.3:43431498-43431517 | MS.gene053895:CDS | 45.0% | |
| TCACCACTGACATTCCATTG+TGG | - | chr5.3:43431504-43431523 | None:intergenic | 45.0% | |
| !! | CTGGCATTTCTAAGATGTGC+AGG | + | chr5.3:43431335-43431354 | MS.gene053895:intron | 45.0% |
| AAAGGATCTGGTAAAGGGGG+AGG | + | chr5.3:43430743-43430762 | MS.gene053895:CDS | 50.0% | |
| AAATTCAGATTCCCGGCCTG+GGG | - | chr5.3:43430804-43430823 | None:intergenic | 50.0% | |
| CAAATTCAGATTCCCGGCCT+GGG | - | chr5.3:43430805-43430824 | None:intergenic | 50.0% | |
| GGGGTGGCTTTCTCAATAAC+TGG | - | chr5.3:43430785-43430804 | None:intergenic | 50.0% | |
| GTTATTGAGAAAGCCACCCC+AGG | + | chr5.3:43430785-43430804 | MS.gene053895:CDS | 50.0% | |
| TCAAATTCAGATTCCCGGCC+TGG | - | chr5.3:43430806-43430825 | None:intergenic | 50.0% | |
| TAAAGGGGGAGGAGGAATCC+TGG | + | chr5.3:43430754-43430773 | MS.gene053895:CDS | 55.0% | |
| GGATCTGGTAAAGGGGGAGG+AGG | + | chr5.3:43430746-43430765 | MS.gene053895:CDS | 60.0% | |
| TTGAGAAAGCCACCCCAGGC+CGG | + | chr5.3:43430789-43430808 | MS.gene053895:CDS | 60.0% | |
| TGAGAAAGCCACCCCAGGCC+GGG | + | chr5.3:43430790-43430809 | MS.gene053895:CDS | 65.0% | |
| TTCAGATTCCCGGCCTGGGG+TGG | - | chr5.3:43430801-43430820 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 43430728 | 43431618 | 43430728 | ID=MS.gene053895 |
| chr5.3 | mRNA | 43430728 | 43431618 | 43430728 | ID=MS.gene053895.t1;Parent=MS.gene053895 |
| chr5.3 | exon | 43430728 | 43430831 | 43430728 | ID=MS.gene053895.t1.exon1;Parent=MS.gene053895.t1 |
| chr5.3 | CDS | 43430728 | 43430831 | 43430728 | ID=cds.MS.gene053895.t1;Parent=MS.gene053895.t1 |
| chr5.3 | exon | 43431357 | 43431618 | 43431357 | ID=MS.gene053895.t1.exon2;Parent=MS.gene053895.t1 |
| chr5.3 | CDS | 43431357 | 43431618 | 43431357 | ID=cds.MS.gene053895.t1;Parent=MS.gene053895.t1 |
| Gene Sequence |
| Protein sequence |
