Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59874.t1 | KEH19286.1 | 86.3 | 146 | 19 | 1 | 1 | 145 | 1 | 146 | 7.80E-62 | 246.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59874.t1 | A0A072TPZ9 | 86.3 | 146 | 19 | 1 | 1 | 145 | 1 | 146 | 5.6e-62 | 246.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049758 | MS.gene59874 | 0.800444 | 1.44E-48 | -1.69E-46 |
| MS.gene050227 | MS.gene59874 | 0.801437 | 9.01E-49 | -1.69E-46 |
| MS.gene051663 | MS.gene59874 | 0.820029 | 8.51E-53 | -1.69E-46 |
| MS.gene054942 | MS.gene59874 | 0.812895 | 3.37E-51 | -1.69E-46 |
| MS.gene060084 | MS.gene59874 | 0.805189 | 1.51E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59874.t1 | MTR_8g046180 | 93.836 | 146 | 8 | 1 | 1 | 145 | 1 | 146 | 2.63e-96 | 277 |
| MS.gene59874.t1 | MTR_8g046180 | 93.836 | 146 | 8 | 1 | 1 | 145 | 1 | 146 | 1.27e-95 | 278 |
| MS.gene59874.t1 | MTR_8g046180 | 93.836 | 146 | 8 | 1 | 1 | 145 | 1 | 146 | 1.71e-95 | 278 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 39 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAAGTATTTCAGCAAATTC+TGG | 0.215370 | 8.3:+53443596 | None:intergenic |
| GAAGAAGGAAAAGGTGTAAA+AGG | 0.229245 | 8.3:+53443953 | None:intergenic |
| AAGTGATTGTTACGGGAAAA+AGG | 0.270164 | 8.3:+53443908 | None:intergenic |
| GAGTAGAAAAGGAGGTATAT+AGG | 0.369542 | 8.3:+53443877 | None:intergenic |
| TTTGATGAAGTGATTGTTAC+GGG | 0.375482 | 8.3:+53443901 | None:intergenic |
| CCAGAATTTGCTGAAATACT+TGG | 0.378222 | 8.3:-53443596 | MS.gene59874:CDS |
| ACGCGAGAATTCAAACAGTT+TGG | 0.400615 | 8.3:-53443807 | MS.gene59874:CDS |
| GGTGATGGCAGAAGATATTC+AGG | 0.433237 | 8.3:-53443665 | MS.gene59874:CDS |
| ATGTCGTTCTGGCATTTCTC+AGG | 0.437588 | 8.3:+53443855 | None:intergenic |
| GTACCTGAGAATGATGATTA+TGG | 0.446888 | 8.3:-53443752 | MS.gene59874:CDS |
| TCAGGTGGAGGACCATATAA+AGG | 0.453495 | 8.3:-53443647 | MS.gene59874:CDS |
| ATTTGATGAAGTGATTGTTA+CGG | 0.461943 | 8.3:+53443900 | None:intergenic |
| ATATAAAGGCGGGGATGAAA+AGG | 0.474783 | 8.3:-53443633 | MS.gene59874:CDS |
| TTTGGAGCATGTAATGGTAA+TGG | 0.476210 | 8.3:+53443931 | None:intergenic |
| GATATTATTGATGTAGATGA+AGG | 0.500419 | 8.3:-53443698 | MS.gene59874:CDS |
| ATAGATGACGTTGCTTACTC+TGG | 0.502126 | 8.3:+53443541 | None:intergenic |
| GCATTTCTCAGGAGTAGAAA+AGG | 0.502576 | 8.3:+53443866 | None:intergenic |
| ACTACATAACAATGTCGTTC+TGG | 0.506409 | 8.3:+53443844 | None:intergenic |
| GAATTTGCTGAAATACTTGG+TGG | 0.508733 | 8.3:-53443593 | MS.gene59874:CDS |
| ACTCTGGACTGTGCCTTCTC+CGG | 0.509116 | 8.3:+53443557 | None:intergenic |
| TGGTGGTTATCTAGATGATC+CGG | 0.512558 | 8.3:-53443576 | MS.gene59874:CDS |
| CATGTAATGGTAATGGAAGA+AGG | 0.514454 | 8.3:+53443938 | None:intergenic |
| CCACCATAATCATCATTCTC+AGG | 0.518299 | 8.3:+53443749 | None:intergenic |
| AAGAAGGAAAAGGTGTAAAA+GGG | 0.522013 | 8.3:+53443954 | None:intergenic |
| GTGGAGGACCATATAAAGGC+GGG | 0.527561 | 8.3:-53443643 | MS.gene59874:CDS |
| AAAAGGTTTGGAGCATGTAA+TGG | 0.540169 | 8.3:+53443925 | None:intergenic |
| GGCAGAAGATATTCAGGTGG+AGG | 0.560514 | 8.3:-53443659 | MS.gene59874:CDS |
| ATGGTAATGGAAGAAGGAAA+AGG | 0.566706 | 8.3:+53443944 | None:intergenic |
| TTGTTATGTAGTGCTAGAAG+TGG | 0.566730 | 8.3:-53443833 | MS.gene59874:CDS |
| TTATCTAGATGATCCGGAGA+AGG | 0.610544 | 8.3:-53443570 | MS.gene59874:CDS |
| GTAGATGAAGGACAAGATGA+TGG | 0.636384 | 8.3:-53443686 | MS.gene59874:CDS |
| CCTGAGAATGATGATTATGG+TGG | 0.646501 | 8.3:-53443749 | MS.gene59874:CDS |
| CTGAGAATGATGATTATGGT+GGG | 0.649683 | 8.3:-53443748 | MS.gene59874:CDS |
| GAAGGACAAGATGATGGTGA+TGG | 0.665835 | 8.3:-53443680 | MS.gene59874:CDS |
| TTTCTCAGGAGTAGAAAAGG+AGG | 0.674623 | 8.3:+53443869 | None:intergenic |
| GGTGGAGGACCATATAAAGG+CGG | 0.683885 | 8.3:-53443644 | MS.gene59874:CDS |
| TGGAGGACCATATAAAGGCG+GGG | 0.705810 | 8.3:-53443642 | MS.gene59874:CDS |
| GATGGCAGAAGATATTCAGG+TGG | 0.711832 | 8.3:-53443662 | MS.gene59874:CDS |
| CTTTGATATAGACATCAATG+AGG | 0.723579 | 8.3:-53443783 | MS.gene59874:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATTTGATGAAGTGATTGTTA+CGG | + | chr8.3:53443609-53443628 | None:intergenic | 25.0% |
| ! | GATATTATTGATGTAGATGA+AGG | - | chr8.3:53443808-53443827 | MS.gene59874:CDS | 25.0% |
| CTTTGATATAGACATCAATG+AGG | - | chr8.3:53443723-53443742 | MS.gene59874:CDS | 30.0% | |
| TTTGATGAAGTGATTGTTAC+GGG | + | chr8.3:53443608-53443627 | None:intergenic | 30.0% | |
| ! | AAGAAGGAAAAGGTGTAAAA+GGG | + | chr8.3:53443555-53443574 | None:intergenic | 30.0% |
| AAGTGATTGTTACGGGAAAA+AGG | + | chr8.3:53443601-53443620 | None:intergenic | 35.0% | |
| ACTACATAACAATGTCGTTC+TGG | + | chr8.3:53443665-53443684 | None:intergenic | 35.0% | |
| ATTGTTACGGGAAAAAGGTT+TGG | + | chr8.3:53443596-53443615 | None:intergenic | 35.0% | |
| CCAAGTATTTCAGCAAATTC+TGG | + | chr8.3:53443913-53443932 | None:intergenic | 35.0% | |
| CTGAGAATGATGATTATGGT+GGG | - | chr8.3:53443758-53443777 | MS.gene59874:CDS | 35.0% | |
| GTACCTGAGAATGATGATTA+TGG | - | chr8.3:53443754-53443773 | MS.gene59874:CDS | 35.0% | |
| TTGTTATGTAGTGCTAGAAG+TGG | - | chr8.3:53443673-53443692 | MS.gene59874:CDS | 35.0% | |
| ! | AAAAGGTTTGGAGCATGTAA+TGG | + | chr8.3:53443584-53443603 | None:intergenic | 35.0% |
| ! | GAAGAAGGAAAAGGTGTAAA+AGG | + | chr8.3:53443556-53443575 | None:intergenic | 35.0% |
| ! | GAGTAGAAAAGGAGGTATAT+AGG | + | chr8.3:53443632-53443651 | None:intergenic | 35.0% |
| ! | TTTGGAGCATGTAATGGTAA+TGG | + | chr8.3:53443578-53443597 | None:intergenic | 35.0% |
| !! | ATGGTAATGGAAGAAGGAAA+AGG | + | chr8.3:53443565-53443584 | None:intergenic | 35.0% |
| !! | CATGTAATGGTAATGGAAGA+AGG | + | chr8.3:53443571-53443590 | None:intergenic | 35.0% |
| !! | CCAGAATTTGCTGAAATACT+TGG | - | chr8.3:53443910-53443929 | MS.gene59874:CDS | 35.0% |
| !! | GAATTTGCTGAAATACTTGG+TGG | - | chr8.3:53443913-53443932 | MS.gene59874:CDS | 35.0% |
| ACGCGAGAATTCAAACAGTT+TGG | - | chr8.3:53443699-53443718 | MS.gene59874:CDS | 40.0% | |
| ATAGATGACGTTGCTTACTC+TGG | + | chr8.3:53443968-53443987 | None:intergenic | 40.0% | |
| ATATAAAGGCGGGGATGAAA+AGG | - | chr8.3:53443873-53443892 | MS.gene59874:CDS | 40.0% | |
| CCACCATAATCATCATTCTC+AGG | + | chr8.3:53443760-53443779 | None:intergenic | 40.0% | |
| CCTGAGAATGATGATTATGG+TGG | - | chr8.3:53443757-53443776 | MS.gene59874:CDS | 40.0% | |
| GCATTTCTCAGGAGTAGAAA+AGG | + | chr8.3:53443643-53443662 | None:intergenic | 40.0% | |
| GTAGATGAAGGACAAGATGA+TGG | - | chr8.3:53443820-53443839 | MS.gene59874:CDS | 40.0% | |
| TGGTGGTTATCTAGATGATC+CGG | - | chr8.3:53443930-53443949 | MS.gene59874:CDS | 40.0% | |
| TTATCTAGATGATCCGGAGA+AGG | - | chr8.3:53443936-53443955 | MS.gene59874:CDS | 40.0% | |
| TTTCTCAGGAGTAGAAAAGG+AGG | + | chr8.3:53443640-53443659 | None:intergenic | 40.0% | |
| ! | TTTTCATCCCCGCCTTTATA+TGG | + | chr8.3:53443874-53443893 | None:intergenic | 40.0% |
| ATGTCGTTCTGGCATTTCTC+AGG | + | chr8.3:53443654-53443673 | None:intergenic | 45.0% | |
| GATGGCAGAAGATATTCAGG+TGG | - | chr8.3:53443844-53443863 | MS.gene59874:CDS | 45.0% | |
| TCAGGTGGAGGACCATATAA+AGG | - | chr8.3:53443859-53443878 | MS.gene59874:CDS | 45.0% | |
| ! | GAAGGACAAGATGATGGTGA+TGG | - | chr8.3:53443826-53443845 | MS.gene59874:CDS | 45.0% |
| ! | GGTGATGGCAGAAGATATTC+AGG | - | chr8.3:53443841-53443860 | MS.gene59874:CDS | 45.0% |
| !!! | ATGGTGGGTTTTCAGCTCAA+GGG | - | chr8.3:53443773-53443792 | MS.gene59874:CDS | 45.0% |
| !!! | TATGGTGGGTTTTCAGCTCA+AGG | - | chr8.3:53443772-53443791 | MS.gene59874:CDS | 45.0% |
| GGCAGAAGATATTCAGGTGG+AGG | - | chr8.3:53443847-53443866 | MS.gene59874:CDS | 50.0% | |
| GGTGGAGGACCATATAAAGG+CGG | - | chr8.3:53443862-53443881 | MS.gene59874:CDS | 50.0% | |
| GTGGAGGACCATATAAAGGC+GGG | - | chr8.3:53443863-53443882 | MS.gene59874:CDS | 50.0% | |
| TGGAGGACCATATAAAGGCG+GGG | - | chr8.3:53443864-53443883 | MS.gene59874:CDS | 50.0% | |
| ! | TGGGTTTTCAGCTCAAGGGA+TGG | - | chr8.3:53443777-53443796 | MS.gene59874:CDS | 50.0% |
| ACTCTGGACTGTGCCTTCTC+CGG | + | chr8.3:53443952-53443971 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 53443538 | 53443990 | 53443538 | ID=MS.gene59874 |
| chr8.3 | mRNA | 53443538 | 53443990 | 53443538 | ID=MS.gene59874.t1;Parent=MS.gene59874 |
| chr8.3 | exon | 53443538 | 53443990 | 53443538 | ID=MS.gene59874.t1.exon1;Parent=MS.gene59874.t1 |
| chr8.3 | CDS | 53443538 | 53443990 | 53443538 | ID=cds.MS.gene59874.t1;Parent=MS.gene59874.t1 |
| Gene Sequence |
| Protein sequence |
