AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene060084

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060084.t1 MTR_6g059910 97.535 649 16 0 64 712 14 662 0.0 1293
MS.gene060084.t1 MTR_3g091550 75.744 437 98 4 281 712 148 581 0.0 583
MS.gene060084.t1 MTR_4g134610 67.797 118 35 1 364 481 74 188 4.01e-43 155
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060084.t1 AT1G33360 60.597 637 209 7 59 690 39 638 0.0 734
MS.gene060084.t1 AT5G53350 72.128 470 123 3 229 695 96 560 0.0 663
MS.gene060084.t1 AT5G49840 67.221 421 125 5 296 712 197 608 0.0 559
MS.gene060084.t1 AT5G49840 46.835 79 29 1 81 159 77 142 6.57e-18 88.6
MS.gene060084.t1 AT5G49840 66.983 421 126 5 296 712 197 608 0.0 557
MS.gene060084.t1 AT5G49840 46.835 79 29 1 81 159 77 142 6.57e-18 88.6
MS.gene060084.t1 AT5G49840 70.153 392 113 3 296 683 197 588 0.0 556
MS.gene060084.t1 AT5G49840 46.835 79 29 1 81 159 77 142 8.85e-18 88.2
MS.gene060084.t1 AT5G49840 71.315 251 69 2 296 543 197 447 4.60e-115 355
MS.gene060084.t1 AT5G49840 46.835 79 29 1 81 159 77 142 1.41e-17 86.7

Find 202 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCGGGACCCACATGGAATT+TGG 0.171857 6.2:-68399338 MS.gene060084:CDS
AATATCAGCCGAGATGTTTC+TGG 0.210145 6.2:-68396669 MS.gene060084:CDS
GGTCTTGATAAGTTTGTTAT+TGG 0.210515 6.2:-68398447 MS.gene060084:CDS
CAAGGTACATTTGTTGAAAT+TGG 0.223334 6.2:-68398912 MS.gene060084:CDS
CAAGATTCTTCTATTGGTTT+TGG 0.233687 6.2:-68395386 MS.gene060084:CDS
TGCATGGGGAGGGTCTAATT+TGG 0.234573 6.2:-68398505 MS.gene060084:CDS
AATGCTGGTGGTGGTAATAA+TGG 0.276535 6.2:-68398690 MS.gene060084:CDS
GGTCTCATACCAGAGTTTAT+TGG 0.285611 6.2:-68394882 MS.gene060084:CDS
ACTAGGTCGGGTGCAGATTC+AGG 0.291303 6.2:-68397763 MS.gene060084:intron
GCATGGGGAGGGTCTAATTT+GGG 0.311540 6.2:-68398504 MS.gene060084:CDS
GGAAGAACAACATACCTTCT+TGG 0.313775 6.2:+68398413 None:intergenic
CAAAGTAATGGTGGTGATTC+CGG 0.321215 6.2:-68399050 MS.gene060084:CDS
GGGGCCGCAGGAGGGTGGTC+CGG 0.325193 6.2:-68398553 MS.gene060084:CDS
GAGGGTGGTCCGGGCGGTGG+TGG 0.325608 6.2:-68398543 MS.gene060084:CDS
CAGGAGGGTGGTCCGGGCGG+TGG 0.331285 6.2:-68398546 MS.gene060084:CDS
TTAACTAGAAGGTTTAAATA+TGG 0.331920 6.2:-68398849 MS.gene060084:CDS
GGCGGAAACGGCGAAAATTT+AGG 0.338401 6.2:-68399026 MS.gene060084:CDS
AGACGGCAAGATTCTTCTAT+TGG 0.351882 6.2:-68395392 MS.gene060084:CDS
AGTAGCTGAATCAGAGTTAC+AGG 0.352303 6.2:-68391261 MS.gene060084:CDS
ATGCTGGTGGTGGTAATAAT+GGG 0.352334 6.2:-68398689 MS.gene060084:CDS
GGTCCGGGCGGTGGTGGTAA+TGG 0.353545 6.2:-68398537 MS.gene060084:CDS
GGTGCAGATTCAGGAATTTC+AGG 0.353959 6.2:-68397754 MS.gene060084:CDS
AAACCGGTTCAACCGCGTTC+AGG 0.357913 6.2:-68399282 MS.gene060084:CDS
GGGCCGCAGGAGGGTGGTCC+GGG 0.361640 6.2:-68398552 MS.gene060084:CDS
AGTGAGGATTTAGGTCTAAA+TGG 0.362272 6.2:-68398783 MS.gene060084:CDS
ACAATGCCGGCGGGTGGCGG+TGG 0.364775 6.2:-68399213 MS.gene060084:CDS
AGAAGGTTTAAATATGGTAT+TGG 0.366946 6.2:-68398843 MS.gene060084:CDS
TAAATGGCAGTGCTAGTAAT+TGG 0.367367 6.2:-68398767 MS.gene060084:CDS
CACCTGGGATTAATGTTGTA+AGG 0.367830 6.2:-68398581 MS.gene060084:CDS
GTAAAACTCAATTAACTAGA+AGG 0.367917 6.2:-68398860 MS.gene060084:CDS
GGCGAAAATTTAGGTTCTAA+TGG 0.372258 6.2:-68399017 MS.gene060084:CDS
AGTTCTGATCTTATTGCATA+TGG 0.374394 6.2:-68394903 MS.gene060084:CDS
CATCATGGAAGTGAGGATTT+AGG 0.375770 6.2:-68398792 MS.gene060084:CDS
GGCGGTGGTGGTGGGAATGC+TGG 0.379824 6.2:-68398705 MS.gene060084:CDS
TCCACGGGGTGATAACATTC+AGG 0.381577 6.2:-68395853 MS.gene060084:intron
CATAAGTTGCTCTTCGGTTA+AGG 0.383500 6.2:+68394830 None:intergenic
GTCGCTTCCTGTCTTAATAT+CGG 0.385991 6.2:+68392170 None:intergenic
CTTCTAATGTTGGCACGAAC+TGG 0.390138 6.2:+68395359 None:intergenic
ACCCACATGGAATTTGGTTC+AGG 0.391674 6.2:-68399332 MS.gene060084:CDS
TCGCCTGAACGCGGTTGAAC+CGG 0.402699 6.2:+68399279 None:intergenic
AGCATCATCTTTACTTGAAT+CGG 0.406039 6.2:-68395312 MS.gene060084:intron
CGCCGGTAGGTGGTGGCAAT+GGG 0.408892 6.2:-68398644 MS.gene060084:CDS
AATTACAATGGCGGTGGTGG+TGG 0.416026 6.2:-68398714 MS.gene060084:CDS
AGACCTTTACAAATCTCCTT+TGG 0.416275 6.2:+68398465 None:intergenic
AAAGGATTACCTCTCTGAAA+TGG 0.417863 6.2:+68395524 None:intergenic
GGTTCAGGTAACCGTCGAAC+CGG 0.418230 6.2:-68399317 MS.gene060084:CDS
TTCTGGTGAGGGTGTTCAGC+AGG 0.419447 6.2:-68396652 MS.gene060084:CDS
ATAACCCGACCCGGAAAATC+CGG 0.421552 6.2:-68399136 MS.gene060084:CDS
GGTGATTCCGGCGGCGGAAA+CGG 0.422136 6.2:-68399038 MS.gene060084:CDS
CCGGCATTGTTTGCCACCTC+CGG 0.428221 6.2:+68399226 None:intergenic
ACCGGCGGTGGCCATGTCTC+TGG 0.429640 6.2:+68398660 None:intergenic
CAAACAATGCCGGCGGGTGG+CGG 0.439337 6.2:-68399216 MS.gene060084:CDS
TGTAATGGTGATGTATTATA+CGG 0.440406 6.2:+68398933 None:intergenic
TTTAACCGATGCCATGTATG+AGG 0.441190 6.2:-68392287 MS.gene060084:intron
CCGGGTTCACTTTATTAAAC+CGG 0.441691 6.2:-68399298 MS.gene060084:CDS
CCGCCGGTAGGTGGTGGCAA+TGG 0.442297 6.2:-68398645 MS.gene060084:CDS
ACATTAATCCCAGGTGCAAA+AGG 0.444033 6.2:+68398588 None:intergenic
TCAACAAATGTACCTTGTAA+TGG 0.444954 6.2:+68398918 None:intergenic
GGTAGAGTGAGTAATAATAA+TGG 0.445653 6.2:-68398891 MS.gene060084:CDS
TGTCTAGATAGTAAATCTTC+CGG 0.446861 6.2:-68395895 MS.gene060084:intron
GGCTGACAAGCTAACTAAGA+TGG 0.447213 6.2:+68394851 None:intergenic
TTGATGGGTCCAACAGGATC+AGG 0.448560 6.2:-68397682 MS.gene060084:intron
TGGGGCCGTGGTGGGTCGGA+TGG 0.449326 6.2:-68399167 MS.gene060084:CDS
TGTTTACCAAGAGCATGTTT+TGG 0.455263 6.2:+68392513 None:intergenic
TGCCGGCGGGTGGCGGTGGT+CGG 0.456846 6.2:-68399209 MS.gene060084:CDS
TCTTCGCGGAGATGGTGCTT+TGG 0.461133 6.2:-68392076 MS.gene060084:CDS
CAAGTGGGGCCGTGGTGGGT+CGG 0.461546 6.2:-68399171 MS.gene060084:CDS
ATGAATACTTGCCTGTGTTA+AGG 0.462855 6.2:+68397494 None:intergenic
GCAGTACCTAGCTAAGATAA+AGG 0.467142 6.2:-68392052 MS.gene060084:CDS
AAGGAAATGGCGTCATTTCG+CGG 0.467697 6.2:-68399356 MS.gene060084:CDS
ATTACTTTGCATATGACGAT+TGG 0.468868 6.2:+68399064 None:intergenic
CACATCGTCGTCGCCGGAGG+TGG 0.469426 6.2:-68399239 MS.gene060084:CDS
ATTAATGTTGTAAGGGCTAG+GGG 0.478634 6.2:-68398573 MS.gene060084:CDS
TGGCCACCGCCGGTAGGTGG+TGG 0.480741 6.2:-68398651 MS.gene060084:CDS
AACTAACCCTTTCGGTAAAG+TGG 0.481023 6.2:+68397953 None:intergenic
AGTTGACAAGATTACTAAGA+AGG 0.483209 6.2:-68396786 MS.gene060084:intron
TCGCTTCCTGTCTTAATATC+GGG 0.487087 6.2:+68392171 None:intergenic
GGTGGTGGTGGGAATGCTGG+TGG 0.487115 6.2:-68398702 MS.gene060084:CDS
TTCCCATTGCCACCACCTAC+CGG 0.487592 6.2:+68398642 None:intergenic
TGGTGGCAATGGGAATGGTT+TGG 0.489942 6.2:-68398634 MS.gene060084:CDS
CCGCAGGAGGGTGGTCCGGG+CGG 0.494534 6.2:-68398549 MS.gene060084:CDS
TGAATCAGAGTTACAGGATA+GGG 0.494926 6.2:-68391255 MS.gene060084:CDS
GGATTAATGTTGTAAGGGCT+AGG 0.495115 6.2:-68398575 MS.gene060084:CDS
TACCTGCATAAGTTGCTCTT+CGG 0.497540 6.2:+68394824 None:intergenic
TCACTATCAGCTCCAGGATG+TGG 0.499467 6.2:-68392108 MS.gene060084:CDS
AGTAAAAGAACTAACCCTTT+CGG 0.501191 6.2:+68397945 None:intergenic
GCTGACAAGCTAACTAAGAT+GGG 0.502552 6.2:+68394852 None:intergenic
GGTGGTGGGAATGCTGGTGG+TGG 0.505584 6.2:-68398699 MS.gene060084:CDS
CCAGGTGCAAAAGGAGGACC+TGG 0.506343 6.2:+68398597 None:intergenic
TATTCTACCGCTTCTAATGT+TGG 0.511712 6.2:+68395349 None:intergenic
GGTACAAGTGGGGCCGTGGT+GGG 0.513008 6.2:-68399175 MS.gene060084:CDS
GTAGGTGGTGGCAATGGGAA+TGG 0.513024 6.2:-68398639 MS.gene060084:CDS
CCGGAGGTGGCAAACAATGC+CGG 0.513244 6.2:-68399226 MS.gene060084:CDS
AGGTACAAGTGGGGCCGTGG+TGG 0.513332 6.2:-68399176 MS.gene060084:CDS
ACCATGCCACTTTACCGAAA+GGG 0.513748 6.2:-68397959 MS.gene060084:intron
AATGGTGGTGATTCCGGCGG+CGG 0.515429 6.2:-68399044 MS.gene060084:CDS
ATAGTAAATCTTCCGGAGAA+AGG 0.517235 6.2:-68395888 MS.gene060084:CDS
GTTCAGGTAACCGTCGAACC+GGG 0.518609 6.2:-68399316 MS.gene060084:CDS
TTGGCTGTACATACTCCTCC+AGG 0.518736 6.2:-68398615 MS.gene060084:CDS
TATTAAGGCTCTTAAACCCC+TGG 0.520946 6.2:+68392318 None:intergenic
GAAAGGTACAAGTGGGGCCG+TGG 0.522337 6.2:-68399179 MS.gene060084:CDS
AGAAATTACAATGGCGGTGG+TGG 0.525820 6.2:-68398717 MS.gene060084:CDS
CCAGATCAATACCTCATACA+TGG 0.530171 6.2:+68392276 None:intergenic
TTAGGTTCTAATGGTGATGG+TGG 0.530173 6.2:-68399008 MS.gene060084:CDS
ACAGAACCAAAACATGCTCT+TGG 0.532014 6.2:-68392519 MS.gene060084:CDS
AACCGAAGAGCAACTTATGC+AGG 0.532015 6.2:-68394826 MS.gene060084:intron
TTACAAATCTCCTTTGGTGA+AGG 0.533110 6.2:+68398471 None:intergenic
AGCCGAGATGTTTCTGGTGA+GGG 0.533599 6.2:-68396663 MS.gene060084:CDS
CTGAATCCTTTATCTTAGCT+AGG 0.533656 6.2:+68392046 None:intergenic
CTGAATCACATCGTCGTCGC+CGG 0.534974 6.2:-68399245 MS.gene060084:CDS
TCACCAAAGGAGATTTGTAA+AGG 0.535509 6.2:-68398468 MS.gene060084:CDS
ACTATAATGTTACATGCTCA+TGG 0.536473 6.2:+68391211 None:intergenic
GACCCGGAAAATCCGGGCTG+AGG 0.537027 6.2:-68399129 MS.gene060084:CDS
CTGTGTTAAGGTTGTAGCAT+CGG 0.538448 6.2:+68397506 None:intergenic
AGTATTAAGAGTGGTCATCA+TGG 0.538727 6.2:-68398807 MS.gene060084:CDS
CTCCGACCACCGCCACCCGC+CGG 0.539473 6.2:+68399207 None:intergenic
GATTAATGTTGTAAGGGCTA+GGG 0.541289 6.2:-68398574 MS.gene060084:CDS
TTGCCACCACCTACCGGCGG+TGG 0.541369 6.2:+68398648 None:intergenic
GTAGGATCACTATCAGCTCC+AGG 0.543376 6.2:-68392114 MS.gene060084:CDS
TGGCAAACAATGCCGGCGGG+TGG 0.545347 6.2:-68399219 MS.gene060084:CDS
AATTTAGGTTCTAATGGTGA+TGG 0.550448 6.2:-68399011 MS.gene060084:CDS
CTGAATCAGAGTTACAGGAT+AGG 0.553897 6.2:-68391256 MS.gene060084:CDS
AATCGTCATATGCAAAGTAA+TGG 0.555241 6.2:-68399062 MS.gene060084:CDS
TAACCCGACCCGGAAAATCC+GGG 0.555787 6.2:-68399135 MS.gene060084:CDS
CCGGTTTAATAAAGTGAACC+CGG 0.558513 6.2:+68399298 None:intergenic
ATAAAGTGAACCCGGTTCGA+CGG 0.559005 6.2:+68399306 None:intergenic
GAAGGATTTGCCTTCACCAA+AGG 0.562550 6.2:-68398481 MS.gene060084:CDS
CACCATGCCACTTTACCGAA+AGG 0.562697 6.2:-68397960 MS.gene060084:CDS
CAGATTCCCGATATTAAGAC+AGG 0.562888 6.2:-68392177 MS.gene060084:CDS
ATTACAATGGCGGTGGTGGT+GGG 0.563135 6.2:-68398713 MS.gene060084:CDS
TTGAGAAATTACAATGGCGG+TGG 0.564158 6.2:-68398720 MS.gene060084:CDS
AGGAAATGGCGTCATTTCGC+GGG 0.564292 6.2:-68399355 MS.gene060084:CDS
GCCAGAGACATGGCCACCGC+CGG 0.564968 6.2:-68398661 MS.gene060084:CDS
TGGACAAGGTAGAGCCAAGA+AGG 0.565290 6.2:-68398427 MS.gene060084:intron
CGGTGGTCGGAGTTCAAGAA+AGG 0.567810 6.2:-68399196 MS.gene060084:CDS
ACCTGGGATTAATGTTGTAA+GGG 0.569494 6.2:-68398580 MS.gene060084:CDS
GTAGCATCGGCAATGACAAA+CGG 0.571154 6.2:+68397519 None:intergenic
AGGTGGCAAACAATGCCGGC+GGG 0.572353 6.2:-68399222 MS.gene060084:CDS
ATCTTCCGGAGAAAGGAGCC+AGG 0.574643 6.2:-68395881 MS.gene060084:CDS
AAACAGTACAAGAAATTAGT+CGG 0.574698 6.2:-68392495 MS.gene060084:CDS
CACCCTCACCAGAAACATCT+CGG 0.574800 6.2:+68396661 None:intergenic
CATGGGGAGGGTCTAATTTG+GGG 0.582506 6.2:-68398503 MS.gene060084:CDS
GCCCTTACAACATTAATCCC+AGG 0.585045 6.2:+68398579 None:intergenic
ATGGGGAGCCGCCAGAGACA+TGG 0.585081 6.2:-68398671 MS.gene060084:CDS
ACCCTTTCGGTAAAGTGGCA+TGG 0.587487 6.2:+68397958 None:intergenic
GAGAGCAATAGTATTAAGAG+TGG 0.588209 6.2:-68398816 MS.gene060084:CDS
GGGAATCGTCCAATAAACTC+TGG 0.588253 6.2:+68394873 None:intergenic
GTTGTTGATGAAGAATCAGT+AGG 0.595178 6.2:-68392132 MS.gene060084:CDS
AGCTAAGATAAAGGATTCAG+AGG 0.595722 6.2:-68392043 MS.gene060084:intron
GTCATTTCGCGGGACCCACA+TGG 0.596667 6.2:-68399345 MS.gene060084:CDS
CAGCCGAGATGTTTCTGGTG+AGG 0.600057 6.2:-68396664 MS.gene060084:CDS
ACATGGCCACCGCCGGTAGG+TGG 0.600558 6.2:-68398654 MS.gene060084:CDS
AATCACATCGTCGTCGCCGG+AGG 0.601297 6.2:-68399242 MS.gene060084:CDS
CAATACCTCATACATGGCAT+CGG 0.601448 6.2:+68392282 None:intergenic
TCACCATTACCACCACCGCC+CGG 0.602216 6.2:+68398534 None:intergenic
CGTCATATGCAAAGTAATGG+TGG 0.604007 6.2:-68399059 MS.gene060084:CDS
GGGAGGGTCTAATTTGGGGA+AGG 0.605141 6.2:-68398499 MS.gene060084:CDS
AGAGTGAGTAATAATAATGG+TGG 0.605589 6.2:-68398888 MS.gene060084:CDS
TGATTCTTCATCAACAACTA+CGG 0.607482 6.2:+68392137 None:intergenic
GAGACATGGCCACCGCCGGT+AGG 0.614301 6.2:-68398657 MS.gene060084:CDS
GTGAGAAGAATGCATGGGGA+GGG 0.615617 6.2:-68398515 MS.gene060084:CDS
CTATCAGCTCCAGGATGTGG+AGG 0.618119 6.2:-68392105 MS.gene060084:CDS
AGTTCAAGAAAGGTACAAGT+GGG 0.618171 6.2:-68399186 MS.gene060084:CDS
CCGCCCGGACCACCCTCCTG+CGG 0.618646 6.2:+68398549 None:intergenic
GTGGACAATTAGCCTCAGCC+CGG 0.619101 6.2:+68399117 None:intergenic
TATAATACATCACCATTACA+AGG 0.622701 6.2:-68398930 MS.gene060084:CDS
AATGTTATACCTGATCCTGT+TGG 0.626529 6.2:+68397673 None:intergenic
GAGTTCAAGAAAGGTACAAG+TGG 0.626887 6.2:-68399187 MS.gene060084:CDS
GAGTGGTCATCATGGAAGTG+AGG 0.633503 6.2:-68398799 MS.gene060084:CDS
GAGTTGAAATCGCCTGAACG+CGG 0.635875 6.2:+68399270 None:intergenic
GTAATGGTGAGAAGAATGCA+TGG 0.637507 6.2:-68398521 MS.gene060084:CDS
GATAAGTTTGTTATTGGACA+AGG 0.639549 6.2:-68398441 MS.gene060084:CDS
GGGCTGAGGCTAATTGTCCA+CGG 0.640912 6.2:-68399115 MS.gene060084:CDS
ACCTGAACCAAATTCCATGT+GGG 0.644247 6.2:+68399331 None:intergenic
TAATGGTGAGAAGAATGCAT+GGG 0.644865 6.2:-68398520 MS.gene060084:CDS
GTTCGTGCCAACATTAGAAG+CGG 0.646985 6.2:-68395356 MS.gene060084:CDS
GTGATATGCATAACCCGACC+CGG 0.648968 6.2:-68399145 MS.gene060084:CDS
TTGGCTTGAAGTGTCAATGT+GGG 0.651884 6.2:-68398748 MS.gene060084:CDS
GGTGAGAAGAATGCATGGGG+AGG 0.654081 6.2:-68398516 MS.gene060084:CDS
TTAATCCCAGGTGCAAAAGG+AGG 0.655193 6.2:+68398591 None:intergenic
TATCACCATCCGACCCACCA+CGG 0.665482 6.2:+68399162 None:intergenic
CGATGCTACAACCTTAACAC+AGG 0.665547 6.2:-68397505 MS.gene060084:intron
TACCTGAACCAAATTCCATG+TGG 0.665846 6.2:+68399330 None:intergenic
ACTTTGAGAAATTACAATGG+CGG 0.666968 6.2:-68398723 MS.gene060084:CDS
ACCTGAATGTTATCACCCCG+TGG 0.667143 6.2:+68395852 None:intergenic
AATGATAGCGAAGAAAGCAA+TGG 0.670703 6.2:-68392356 MS.gene060084:CDS
AATGTTGCAGCAGCTCAACA+AGG 0.670896 6.2:-68396827 MS.gene060084:CDS
GAGGTGGCAAACAATGCCGG+CGG 0.671349 6.2:-68399223 MS.gene060084:CDS
AATACTATATAAGCTACTTG+CGG 0.672585 6.2:-68397297 MS.gene060084:intron
GGCGGTGGCCATGTCTCTGG+CGG 0.672785 6.2:+68398663 None:intergenic
TGCTGGTGGTGGTAATAATG+GGG 0.676168 6.2:-68398688 MS.gene060084:CDS
CTGACAAGCTAACTAAGATG+GGG 0.678326 6.2:+68394853 None:intergenic
CCATTGCCACCACCTACCGG+CGG 0.691662 6.2:+68398645 None:intergenic
AATGGTGAGAAGAATGCATG+GGG 0.695640 6.2:-68398519 MS.gene060084:CDS
AGTAATGGTGGTGATTCCGG+CGG 0.695700 6.2:-68399047 MS.gene060084:CDS
GGTGCAAAAGGAGGACCTGG+AGG 0.695787 6.2:+68398600 None:intergenic
TTCATATCTTTGGAACACCG+TGG 0.701650 6.2:+68399098 None:intergenic
GTTCAAGAAAGGTACAAGTG+GGG 0.709770 6.2:-68399185 MS.gene060084:CDS
GAAACTTTGAGAAATTACAA+TGG 0.710583 6.2:-68398726 MS.gene060084:CDS
ATTGGCTTGAAGTGTCAATG+TGG 0.726659 6.2:-68398749 MS.gene060084:CDS
TTAATGTTGTAAGGGCTAGG+GGG 0.730354 6.2:-68398572 MS.gene060084:CDS
CAACAACTACGGCGTCAATG+CGG 0.734475 6.2:+68392148 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr6.2 gene 68391220 68399381 68391220 ID=MS.gene060084
chr6.2 mRNA 68391220 68399381 68391220 ID=MS.gene060084.t1;Parent=MS.gene060084
chr6.2 exon 68398428 68399381 68398428 ID=MS.gene060084.t1.exon1;Parent=MS.gene060084.t1
chr6.2 CDS 68398428 68399381 68398428 ID=cds.MS.gene060084.t1;Parent=MS.gene060084.t1
chr6.2 exon 68397960 68398021 68397960 ID=MS.gene060084.t1.exon2;Parent=MS.gene060084.t1
chr6.2 CDS 68397960 68398021 68397960 ID=cds.MS.gene060084.t1;Parent=MS.gene060084.t1
chr6.2 exon 68397683 68397780 68397683 ID=MS.gene060084.t1.exon3;Parent=MS.gene060084.t1
chr6.2 CDS 68397683 68397780 68397683 ID=cds.MS.gene060084.t1;Parent=MS.gene060084.t1
chr6.2 exon 68397506 68397585 68397506 ID=MS.gene060084.t1.exon4;Parent=MS.gene060084.t1
chr6.2 CDS 68397506 68397585 68397506 ID=cds.MS.gene060084.t1;Parent=MS.gene060084.t1
chr6.2 exon 68397298 68397348 68397298 ID=MS.gene060084.t1.exon5;Parent=MS.gene060084.t1
chr6.2 CDS 68397298 68397348 68397298 ID=cds.MS.gene060084.t1;Parent=MS.gene060084.t1
chr6.2 exon 68396787 68396861 68396787 ID=MS.gene060084.t1.exon6;Parent=MS.gene060084.t1
chr6.2 CDS 68396787 68396861 68396787 ID=cds.MS.gene060084.t1;Parent=MS.gene060084.t1
chr6.2 exon 68396626 68396703 68396626 ID=MS.gene060084.t1.exon7;Parent=MS.gene060084.t1
chr6.2 CDS 68396626 68396703 68396626 ID=cds.MS.gene060084.t1;Parent=MS.gene060084.t1
chr6.2 exon 68395854 68395910 68395854 ID=MS.gene060084.t1.exon8;Parent=MS.gene060084.t1
chr6.2 CDS 68395854 68395910 68395854 ID=cds.MS.gene060084.t1;Parent=MS.gene060084.t1
chr6.2 exon 68395534 68395604 68395534 ID=MS.gene060084.t1.exon9;Parent=MS.gene060084.t1
chr6.2 CDS 68395534 68395604 68395534 ID=cds.MS.gene060084.t1;Parent=MS.gene060084.t1
chr6.2 exon 68395313 68395412 68395313 ID=MS.gene060084.t1.exon10;Parent=MS.gene060084.t1
chr6.2 CDS 68395313 68395412 68395313 ID=cds.MS.gene060084.t1;Parent=MS.gene060084.t1
chr6.2 exon 68394827 68394931 68394827 ID=MS.gene060084.t1.exon11;Parent=MS.gene060084.t1
chr6.2 CDS 68394827 68394931 68394827 ID=cds.MS.gene060084.t1;Parent=MS.gene060084.t1
chr6.2 exon 68392485 68392547 68392485 ID=MS.gene060084.t1.exon12;Parent=MS.gene060084.t1
chr6.2 CDS 68392485 68392547 68392485 ID=cds.MS.gene060084.t1;Parent=MS.gene060084.t1
chr6.2 exon 68392288 68392410 68392288 ID=MS.gene060084.t1.exon13;Parent=MS.gene060084.t1
chr6.2 CDS 68392288 68392410 68392288 ID=cds.MS.gene060084.t1;Parent=MS.gene060084.t1
chr6.2 exon 68392044 68392196 68392044 ID=MS.gene060084.t1.exon14;Parent=MS.gene060084.t1
chr6.2 CDS 68392044 68392196 68392044 ID=cds.MS.gene060084.t1;Parent=MS.gene060084.t1
chr6.2 exon 68391220 68391288 68391220 ID=MS.gene060084.t1.exon15;Parent=MS.gene060084.t1
chr6.2 CDS 68391220 68391288 68391220 ID=cds.MS.gene060084.t1;Parent=MS.gene060084.t1
Gene Sequence

>MS.gene060084

ATGAAGGAAATGGCGTCATTTCGCGGGACCCACATGGAATTTGGTTCAGGTAACCGTCGAACCGGGTTCACTTTATTAAACCGGTTCAACCGCGTTCAGGCGATTTCAACTCATCTGAATCACATCGTCGTCGCCGGAGGTGGCAAACAATGCCGGCGGGTGGCGGTGGTCGGAGTTCAAGAAAGGTACAAGTGGGGCCGTGGTGGGTCGGATGGTGATATGCATAACCCGACCCGGAAAATCCGGGCTGAGGCTAATTGTCCACGGTGTTCCAAAGATATGAATTTGTTTTTCTCCAATCGTCATATGCAAAGTAATGGTGGTGATTCCGGCGGCGGAAACGGCGAAAATTTAGGTTCTAATGGTGATGGTGGTTATCAAGCAGTGAATTTATGTCCAAATTGTAAAACAGCGTATCATTTTCGACCGTATAATACATCACCATTACAAGGTACATTTGTTGAAATTGGTAGAGTGAGTAATAATAATGGTGGTAATAGTAAAACTCAATTAACTAGAAGGTTTAAATATGGTATTGGAAAAGAGAGCAATAGTATTAAGAGTGGTCATCATGGAAGTGAGGATTTAGGTCTAAATGGCAGTGCTAGTAATTGGCTTGAAGTGTCAATGTGGGAAACTTTGAGAAATTACAATGGCGGTGGTGGTGGGAATGCTGGTGGTGGTAATAATGGGGAGCCGCCAGAGACATGGCCACCGCCGGTAGGTGGTGGCAATGGGAATGGTTTGGCTGTACATACTCCTCCAGGTCCTCCTTTTGCACCTGGGATTAATGTTGTAAGGGCTAGGGGGCCGCAGGAGGGTGGTCCGGGCGGTGGTGGTAATGGTGAGAAGAATGCATGGGGAGGGTCTAATTTGGGGAAGGATTTGCCTTCACCAAAGGAGATTTGTAAAGGTCTTGATAAGTTTGTTATTGGACAAGGTAGAGCCAAGAAGGTATGTTGTTCTTCCCAAATCCATTTAATGTTATCAATACTGGCGGAAATACGGGCATGTGTGCTATTGCGTGCCCGTTTGTCTGTTTTCTATTGTGCTTTGTTGTATATATGTTCTATCGTATAGGATATTCATTTTAATTTAATTTGCATGTTTCAGTTTCATAATATCGATGTTATTTGAACTTATCTAATTTTAGTTTCAATAATCACGTTGTAAGTTTTAATTATACATTTGGAAAGGAATTGTGTTGTTAAGGCAAATCTGTATAGAAAATTTTTCGATCATATTTGTCTATCTATTTACTATTTTCAAGACATATTGCTAATGCATAATGCAAATGCATTTTCTAACTTATTGCCTTTAATTGTACCTCATTAATTAATGGTTTATGTTTCTGTTGAAGGTGCTTTCTGTAGCTGTTTATAATCACTATAAAAGAATACACCATGCCACTTTACCGAAAGGGTTAGTTCTTTTACTTTCACTCTCATTTTCTTTATTCACGTTCCTCGGTTGAATTTCAACTGATGCTTCTGATTATATGTGATGATAAATGTCTGGAAAATTTACGGAATGATAAACTTATAGTTTGTTCTTTTGCATTATCTATTTTATGTGATTGAAATGGTGGATTTCAATACTAGGTCGGGTGCAGATTCAGGAATTTCAGGCATATTAGATGATGACGAAAATGTTGAGCTAGAAAAGAGTAACGTTCTTTTGATGGGTCCAACAGGATCAGGTATAACATTCATTACAACTAGTTTATAAAACGAAAATAACATTGATGCACCGTTCATATCAGTATCGGCTAATCTAATCTAACTTTCATCATATAGGGAAGACATTACTTGCAAAAACATTAGCTCGATTTGTGAATGTTCCGTTTGTCATTGCCGATGCTACAACCTTAACACAGGCAAGTATTCATTTATTATCAGATAATATTAGAAATTCGTAAACCAAGTGTTCAGCATAAAAAAGTTAAAAAATAATTATTCAGATTTTTACTTATTTTGTCTTGGGACGAAATTTCTGTTGTGTTAACTTGAAATATGAAATGTTCTGATTTTCAGGCTGGTTATGTTGGAGAAGATGTCGAATCAATACTATATAAGCTACTTGCGGTACGTGTATTGTTCTGCAGGGTTATGTTGTTTGAATAAATTATCAATTCTTTCAATTTTTATGTTGTTTGAATAAATTATCAATTCTTTCAATTTTCACTGTCAATTGTGATCGCAATAAATAGTGGCTTGTTCAAATTCCGCTACTCTCTAGCACTAGCTATAACGCCGCAATAGCTGCTATTTGACAACACTTTGTACTAAATAGCATATTGTAGAACTATAGCGATTTCTCCAAATATCGCTATGCTACAGTACCACTACAGTCGCTGTTCGACATAGTGCCGTATCTTAGAAATTGTCATAATTTTTGTTTTATGTGTGTTTACTTTTTTGTCTAACAGCAGATCTTCTTTGCAAAATTTGTTCTTTCATCTCATTGTTTGTAATTGTACCTTCCTTCTCATTGCTACAACGATTATTTTTTTCTGTATAGGCAGCAGATTATAATGTTGCAGCAGCTCAACAAGGAATAATTTACATTGACGAAGTTGACAAGATTACTAAGAAGGTTATTTGTTTAAAATAATTAGATTAAGTTCAAATTTTCTCATCCATAATAGATTTTAAATGTTATGATATATATTTTTACAGGCTGAGAGCTTAAATATCAGCCGAGATGTTTCTGGTGAGGGTGTTCAGCAGGCATTATTAAAAATGCTCGAAGGAACTGTAAGTAGAGCGATTCCAGATTGCTGTTGTTGCTAATTGGTTTATAATCTCTTTTTTAATGAAATCTGTTATGGGAACTGTTAGTTACTTCAATCTTTTGCATTAGCAGCGCTTGTATCATGACATTGCAAAGATACATCGTTTGTACCACAACTAGAATAAAGAAGCAATTTGTTCACATAGGTTGATTAGGTTCTCGTAGCAATTGCATCATGAGCTTCTATTACATAATACGAGCAAGGATGTTTTAGTTGGTGTTTTACCGATAACAAATATTGTTGATGCATTATGGTAATTTCAATTAGGTTAAGAAAGTACAGAAATAGTATATTATTTTGCTTGGTGGGATCTGAATTTTGAGTTTCGAAGGACAAACAAACACGGTAGTGTATGTTTGGTTTCACATTTGGAGGAGGCAAATTTGTTTCTAGAGGTGCAAATTTTTGAGAGTTCCATAGGTGCATTATTGATTTTGACATGTTTGAATGTTTTCGAGTAAAACTGATTATAACTTGAAGCTAGAATTTGGAGCTTTTAATCTGCAATTGATTTTTAGCCTTGAATTAAACAAACCCCAAAATATCATAATATACTTTTTGTTAAGACATCAATTTATACAAATCAATTCATTGTGTGAACTGAATGTTTTTGAAGCACTAAATTCGTAAATGCCATTCTTACGGAATCCTAAACGCAATGATTTTGTATTGTCTAGATAGTAAATCTTCCGGAGAAAGGAGCCAGGAAAAATCCACGGGGTGATAACATTCAGGTATGTTGTTTTGGAACAAATATCATCTCTTCGCTCCGTTTTGTTTAGACATCTATATGATTAGACAAATTTTCTCATCTTTTTTCTGTTCGTATTTTCTTGAATCCTGATACATTTGTCTGGAAATGCATTTCTATTGTTATGGTTAATAAATCAATATTTTTTCTATTTTGAAAGCTCTAGTTTAATAACACATAAGTTGTTAATATTTTGAATAATTTTCTATTCATCTTCTATAATATTATGCAGATAGACACAAAGAACATTCTTTTTATATGTGGTGGAGCATTTATTGATCTTGAAAAAACCATTTCAGAGAGGTAATCCTTTATGCCTTAGTCTTGAAAAAATATTATTTCTTTCACTGATAATAAATGTTTTACACTCGTATTGGTTTCTGCGGAATATTCGTTAACTTGAATTATCAATCAAATAAACCAGACGGCAAGATTCTTCTATTGGTTTTGGAGCACCAGTTCGTGCCAACATTAGAAGCGGTAGAATAATCGATTCTGCTGTAGCATCATCTTTACTTGAATCGGTGAGAAATTCTCTTTTATTAGCTGAAATTTTATTGACTCGTTTTTGTTTTAATCATTATAAATCAAAATAGATAGACAGATAGATATAAAAGTATACGCATAGTTTAAAAAATTGAAACTCATAAGTAATGCATTCAAGATATTGCAGTGCTGTGTGCTTTACCAAATCCTCCAAGATGCAGCACTGACACTTCAGATTGAGGCCATGCCCAGACACCGGCACGGCACCGACACATATAGTTAAAATCAATTATTCTATTTTTTCATATCATTATGGTGTCGACGTGTCAGTGTCAGTTTCGTGTCCGTGTAGTTGATTGTTATATCTGAGAAATTCCAAATTCCAAATGAGTATAAAACGCTGTGTTAATAATTTTCAGGTGGAAAGTTCTGATCTTATTGCATATGGTCTCATACCAGAGTTTATTGGACGATTCCCCATCTTAGTTAGCTTGTCAGCCTTAACCGAAGAGCAACTTATGCAGGTAATCCCTCATTGTTTATATTGCTTTTGATCCTATCATATCTCTTCCTAATTTCTCTGAACAATTAGTGTTCATAGGATTAACAAAATATGGTTATTTAACATTCGCAAGAACGTGAATGATGATATTTAACAGAAAGCTACAGGATATATATATATGTACATATATATATATATATATATATGTATATATATGTGTACATATATATATATACGTGTACATATATATATATACGTGTACATATATGTATATACGTGTACATATATGTATATACGTGTACATATATGTATATACGTATACATATGTATATATATGTGTATATATGGGGGCTGTTAACTTGCACCCACCTAAAAACACTAAGGTGCAAGTTAACAAGTTGCACCTACTAGTTATTTACCAAAACACCCATGTTTTTCTATTATAAACAAATATTATTTATGCTATTTACAAAAATGTCCTTTTAATTAAAATAAAGTTTAATAGAGACAAAAGGGTGTTTTAGTAAAGTACAAGTAGGTGCAGCTTGTTAACTTGCACCTTAGTGTTTTTAGGTGGGTGCAAGTTAACAGCCCCCACTGTTAACTTGCACCCACCTAAAAACACTAAGGTGCAAGTTAACAAGCTGCACCTACTTGTTATTTACCAAAACACCCATTTTTTCTATTATAAACAAATATTATTTATGCTATTTACAAAAATGTCCTTTTAATTAAAATAAAGTTTAATAGAGACAAAAGGGTGTTTTGGTAAAGTACAAGTAGGTGCAGCTTGTTAACTTGCACCTTAGTGTTTTTAGGTGGGTGCAAGTTAACAGCCCCCTATATATATATATATATATATATATATATGAGATAGGATCCGTTGACACCAGGTGTCAACGAATTCGTTGACACCAAATCTCAACCGTTTATTTCATTTAATCAAAAGGCTCATATTTATTTAATTGAAATCTTACATGATCATTACTTTCCGCATTTTCCCTGATCTTTGTTATCTCTATGGTTTTTCATATCGTTGTTACTTATTATGATGATTGCTCATATTTCCTCATCTCAACCATTGGACGCCTATTCCAAATGGAAGTCGCAGCTTCTTCTTAGTTTCAATTTATTTTACCGGTTCAAATATTAAGGGTGAATTGTGATTTATTTGGCTAAATAAATTCTATTTCAAAATTATAATTGATCAACAAACTAGATTATTTCAGAAATTGATTGTTGTCGGTGATTACATTTTCAATTTTACCTCTCTGTTCGGCGGTATATAGCAGCAATCTTTAGCTTCAATCCCAACAATACCTTCCTCTTTTTATGATGAATGTTCATCGTCTCTCTTCCTTTTTTCATTATACCAATTTGCATTCTAAGCATTAAGGTGAATGGGGAAATTAAAGTTAATGGAATAGAGTATGTGGAACGTTTTTGATTAAAACAAATTAAAATTTATACCCTTTAATTTCTTTGGTCAAATCAAAAACCAAGCCTATAGTATCAATTCTTAATATCATGAGTCCTATTGGTTCACGCACAAAACTCAAGCGGTCAAGCCTATCATATCAATTTTATTATCTATCGTATGCACAAAAGTAACTATCTCTTCTCGCATCCATTTTGACGACGACCAAACAATTCGAATTTGGATGGATTTGTTTTGATTGTTGGTCATTAAATTCGTTGTGTTGAAGCTGATGGAGAAGAAGAAAGAGCGTGTGGATCAGGTGAGTCAAAGCAAACAAGATCACACAAGTTAATTTAAGAAATAATCACGGACCATTAGATTAAACAAGATAGACGGTTGAGATTTGGTGTCAACGAATTCGTTGACACCAGGTGTCAACGGATCCTATCTCTCTCTCTATATATATATATATATATATATATATATGGGGAATGCTAGTTTAGAGTTTAAATTTGTTGTCCATATATATATTTATCCTGCTTGCAAAATGTCGCATATGCTAGTTTAGAGTTTAAATTTGTTGTCACATGTCAGCGATTAGTTACGAACGGTTGCATTAGTTAGTTTCTTGCAACTGTACACATGTTAAGCCTAACCAAATCTATATAAATACAAGTTTAATAGCTGGAATCAGACTATGAAAATCATTTTCATCAGAAAGAATATTAGAATTCTCTCTTGCTTTCTTTTTAAATCTCTAGAACTTTATCAATGTGGAATTCTTGATTTTGGCGTTCTTAAGGAAGATTTATAGTTATTGCTTATTTTAGATTTTGATTTGTAGTAAATATATGACAATGAAAATATTGTTTGGCTTGCAGGTCCTCACAGAACCAAAACATGCTCTTGGTAAACAGTACAAGAAATTAGTCGGCATGAATGAAGTAAGCATCTCCGTTTGTGTGTGTTTTTGCGCGCACGTATTGAATTAGTCTGATACTTTAATCTTGCACTTCAGGTAAAGCTACACTTCACAGAGAAAGCTCTGAGAATGATAGCGAAGAAAGCAATGGCCAAAAATACTGGTGCCAGGGGTTTAAGAGCCTTAATAGAAAACATTTTAACCGATGCCATGTATGAGGTATTGATCTGGCTATCTTCGATCATCAAAAACTGTTTCCAATCTCTGCGGTTTTGATATTAATTACACACAATGTTTATGTGTGCACCAGATTCCCGATATTAAGACAGGAAGCGACCGCATTGACGCCGTAGTTGTTGATGAAGAATCAGTAGGATCACTATCAGCTCCAGGATGTGGAGGAAAAATTCTTCGCGGAGATGGTGCTTTGGAGCAGTACCTAGCTAAGATAAAGGATTCAGAGGTACAATCTTTCATAAAGGCTAATTGACTGATTTTTTTTCTTTTGGGAATGATTATTATAAAAGGCCTATCCTCAATGAGGGTTGACAGAATTGATTAAGGATCCGAGGTTTCACACGTGTATCCATTATATCCATGTAGCAACACATGTCTCCCCTGATAATCTCAGTTGCTACATGGATACACGAGTTGGATACATGGATCCATGTAGCTAAGGAGAGGACTATAATTGAGTAAAAATCGGAGTGAAAATCGGAAGAAACTTTTGTCATCACTTATATCAATCAGATATGTAAATTGTATTTCTGAACATCATATTTTGTCTAGAAGTATACTTCGATTTTTTGACTCAACTAGTTGTGTACTTCTGAACAAAATTTGCGCCTTTCGCTGATGTGTTCATCTGAATTCATGTTGGGATGGAGAAAATTCCTCCCTGAAAATCACATATTCGCAGCCCTAGTATGCCATTGGCACGCTGCCCAGTTGCATGACTTGGTGTCTCCCCTACCTACCACTTTTATTGAGTGGGAAAATAATATAGAATGGCCATAATTTTCTTTACGTAGCTACTCTGAAATTTATGTAAACGGTGCTAAAAAAAGTGAGCCCATCTCGTTGAATTAGATCAACGTAAAATTCATCCTCGATTGTAATGCACTTAACAGAATGTATTGCTAGCATTTCGATGTCTGTTATTTACAATAGTTCTAGGCCATATTTTTGACCATATATTTTTTTCTTAATCTTTTGCAGGATGTAGTAGCTGAATCAGAGTTACAGGATAGGGATTCAGATACTTCATCAAGAGCCATGAGCATGTAA
Protein sequence

>MS.gene060084.t1

MKEMASFRGTHMEFGSGNRRTGFTLLNRFNRVQAISTHLNHIVVAGGGKQCRRVAVVGVQERYKWGRGGSDGDMHNPTRKIRAEANCPRCSKDMNLFFSNRHMQSNGGDSGGGNGENLGSNGDGGYQAVNLCPNCKTAYHFRPYNTSPLQGTFVEIGRVSNNNGGNSKTQLTRRFKYGIGKESNSIKSGHHGSEDLGLNGSASNWLEVSMWETLRNYNGGGGGNAGGGNNGEPPETWPPPVGGGNGNGLAVHTPPGPPFAPGINVVRARGPQEGGPGGGGNGEKNAWGGSNLGKDLPSPKEICKGLDKFVIGQGRAKKVLSVAVYNHYKRIHHATLPKGSGADSGISGILDDDENVELEKSNVLLMGPTGSGKTLLAKTLARFVNVPFVIADATTLTQAGYVGEDVESILYKLLAAADYNVAAAQQGIIYIDEVDKITKKAESLNISRDVSGEGVQQALLKMLEGTIVNLPEKGARKNPRGDNIQIDTKNILFICGGAFIDLEKTISERRQDSSIGFGAPVRANIRSGRIIDSAVASSLLESVESSDLIAYGLIPEFIGRFPILVSLSALTEEQLMQVLTEPKHALGKQYKKLVGMNEVKLHFTEKALRMIAKKAMAKNTGARGLRALIENILTDAMYEIPDIKTGSDRIDAVVVDEESVGSLSAPGCGGKILRGDGALEQYLAKIKDSEDVVAESELQDRDSDTSSRAMSM