AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene60039


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60039.t1 MTR_4g010340 96.585 205 7 0 1 205 1 205 1.09e-142 396
MS.gene60039.t1 MTR_8g020760 71.770 209 55 2 1 205 3 211 1.56e-100 290
MS.gene60039.t1 MTR_8g020763 75.275 182 43 2 26 205 9 190 4.36e-93 270
MS.gene60039.t1 MTR_2g019780 65.728 213 62 5 1 205 3 212 1.30e-86 254
MS.gene60039.t1 MTR_8g020630 61.538 208 76 3 1 205 3 209 4.81e-86 253
MS.gene60039.t1 MTR_8g020670 61.765 204 75 2 5 205 6 209 1.98e-84 249
MS.gene60039.t1 MTR_3g073170 64.929 211 65 4 1 205 3 210 2.21e-84 249
MS.gene60039.t1 MTR_8g020650 63.636 187 67 1 20 205 23 209 3.86e-84 248
MS.gene60039.t1 MTR_2g045100 61.353 207 77 2 2 205 3 209 2.59e-81 241
MS.gene60039.t1 MTR_2g044040 59.135 208 81 3 1 205 3 209 7.72e-81 239
MS.gene60039.t1 MTR_8g020770 58.768 211 80 4 1 205 3 212 3.78e-79 236
MS.gene60039.t1 MTR_2g036120 57.971 207 83 3 1 204 3 208 3.31e-76 228
MS.gene60039.t1 MTR_8g020750 58.571 210 82 4 1 205 3 212 1.50e-75 226
MS.gene60039.t1 MTR_3g019710 55.882 204 87 2 5 205 6 209 8.14e-75 224
MS.gene60039.t1 MTR_0284s0010 56.250 208 87 3 1 205 3 209 1.77e-74 224
MS.gene60039.t1 MTR_7g011340 58.421 190 78 1 17 205 20 209 5.63e-74 222
MS.gene60039.t1 MTR_3g019680 54.589 207 90 3 2 205 3 208 5.90e-72 217
MS.gene60039.t1 MTR_3g019650 54.106 207 91 3 2 205 3 208 1.80e-71 216
MS.gene60039.t1 MTR_5g031460 53.846 208 92 3 1 205 3 209 1.88e-69 211
MS.gene60039.t1 MTR_8g020610 52.913 206 90 5 5 205 4 207 9.68e-67 204
MS.gene60039.t1 MTR_8g020590 50.000 208 101 3 1 205 1 208 4.02e-63 195
MS.gene60039.t1 MTR_2g041550 49.038 208 103 3 1 205 3 210 8.64e-61 189
MS.gene60039.t1 MTR_2g041570 47.115 208 107 3 1 205 3 210 3.69e-58 182
MS.gene60039.t1 MTR_2g041580 47.115 208 107 3 1 205 3 210 1.03e-57 181
MS.gene60039.t1 MTR_3g019810 51.269 197 87 3 5 198 112 302 2.64e-56 181
MS.gene60039.t1 MTR_4g027415 57.724 123 51 1 17 138 20 142 6.23e-41 136
MS.gene60039.t1 MTR_1g085140 40.816 196 107 5 13 201 22 215 2.23e-34 122
MS.gene60039.t1 MTR_1g079490 37.173 191 111 4 20 201 27 217 3.20e-32 116
MS.gene60039.t1 MTR_1g085500 37.143 210 116 7 4 200 7 213 4.36e-32 115
MS.gene60039.t1 MTR_6g005330 38.426 216 117 7 1 201 1 215 7.89e-32 115
MS.gene60039.t1 MTR_6g005310 38.889 216 116 7 1 201 1 215 9.47e-32 115
MS.gene60039.t1 MTR_6g005360 40.805 174 96 4 34 201 43 215 1.19e-31 114
MS.gene60039.t1 MTR_1g060120 32.500 200 126 3 12 202 23 222 1.47e-31 114
MS.gene60039.t1 MTR_7g111240 39.037 187 109 4 20 201 25 211 3.61e-31 113
MS.gene60039.t1 MTR_7g111240 39.572 187 105 5 20 201 25 208 4.87e-31 113
MS.gene60039.t1 MTR_6g005340 38.426 216 117 7 1 201 1 215 6.36e-31 113
MS.gene60039.t1 MTR_2g031270 37.566 189 108 5 20 201 28 213 9.95e-31 112
MS.gene60039.t1 MTR_2g072560 56.364 110 45 2 5 111 6 115 2.73e-30 109
MS.gene60039.t1 MTR_1g112200 33.166 199 127 3 5 201 13 207 1.38e-29 109
MS.gene60039.t1 MTR_2g030855 39.011 182 100 5 20 196 27 202 4.46e-29 108
MS.gene60039.t1 MTR_1g023770 38.596 171 98 4 38 201 39 209 5.96e-29 107
MS.gene60039.t1 MTR_3g019610 56.383 94 40 1 8 101 12 104 3.13e-28 102
MS.gene60039.t1 MTR_4g017040 38.506 174 99 4 34 200 43 215 1.40e-26 101
MS.gene60039.t1 MTR_4g017050 35.945 217 118 7 1 200 3 215 4.96e-26 100
MS.gene60039.t1 MTR_5g046410 35.945 217 118 7 1 200 3 215 5.12e-26 100
MS.gene60039.t1 MTR_5g046430 35.945 217 118 7 1 200 3 215 5.12e-26 100
MS.gene60039.t1 MTR_4g017030 35.945 217 118 7 1 200 3 215 5.12e-26 100
MS.gene60039.t1 MTR_2g019250 38.506 174 99 4 34 200 43 215 7.66e-26 99.8
MS.gene60039.t1 MTR_8g007300 35.673 171 96 6 6 164 11 179 1.51e-25 99.0
MS.gene60039.t1 MTR_2g030895 36.628 172 101 5 38 201 7 178 2.56e-25 97.4
MS.gene60039.t1 MTR_6g005380 40.782 179 89 6 1 164 1 177 8.66e-25 96.7
MS.gene60039.t1 MTR_5g036570 33.511 188 111 5 20 203 32 209 3.81e-24 95.5
MS.gene60039.t1 MTR_2g030865 36.628 172 101 5 38 201 7 178 1.27e-23 92.8
MS.gene60039.t1 MTR_3g096330 33.714 175 103 4 3 164 556 730 2.91e-23 97.1
MS.gene60039.t1 MTR_6g005350 32.558 215 129 6 2 201 1 214 1.38e-21 88.6
MS.gene60039.t1 MTR_2g086630 34.444 180 109 5 30 202 28 205 5.98e-21 86.7
MS.gene60039.t1 MTR_2g031300 45.283 106 53 3 38 138 7 112 1.93e-20 84.0
MS.gene60039.t1 MTR_2g086620 33.014 209 121 7 1 202 9 205 2.72e-20 85.1
MS.gene60039.t1 MTR_2g086640 32.402 179 114 5 30 202 27 204 1.53e-19 82.8
MS.gene60039.t1 MTR_2g096110 38.816 152 68 6 43 190 8 138 3.97e-19 80.1
MS.gene60039.t1 MTR_4g052770 36.585 164 98 4 44 201 42 205 2.18e-18 79.7
MS.gene60039.t1 MTR_4g052780 36.585 164 98 4 44 201 42 205 4.29e-18 79.0
MS.gene60039.t1 MTR_7g029500 68.333 60 15 1 146 205 38 93 3.49e-17 73.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60039.t1 AT1G72610 48.077 208 105 3 1 205 1 208 4.31e-60 187
MS.gene60039.t1 AT5G20630 48.077 208 104 4 1 204 3 210 1.95e-57 180
MS.gene60039.t1 AT5G38960 39.706 204 105 6 12 201 23 222 8.23e-36 125
MS.gene60039.t1 AT3G05950 40.777 206 108 6 10 201 16 221 1.00e-33 120
MS.gene60039.t1 AT1G09560 39.000 200 117 4 8 202 17 216 1.89e-33 119
MS.gene60039.t1 AT3G62020 38.462 208 115 7 7 201 8 215 2.57e-33 119
MS.gene60039.t1 AT5G26700 38.191 199 117 5 6 201 14 209 5.40e-33 118
MS.gene60039.t1 AT1G02335 38.503 187 110 4 20 201 29 215 1.10e-32 117
MS.gene60039.t1 AT3G05930 37.500 208 119 5 5 201 8 215 2.33e-32 116
MS.gene60039.t1 AT5G39190 37.379 206 115 6 10 201 14 219 4.06e-32 116
MS.gene60039.t1 AT5G38940 40.000 175 98 4 34 201 47 221 4.08e-32 116
MS.gene60039.t1 AT1G18980 37.313 201 119 5 6 201 20 218 4.26e-32 116
MS.gene60039.t1 AT5G39160 36.098 205 115 6 10 201 14 215 6.70e-32 115
MS.gene60039.t1 AT5G38940 40.000 175 98 4 34 201 71 245 7.11e-32 116
MS.gene60039.t1 AT5G39160 36.893 206 116 6 10 201 14 219 9.46e-32 115
MS.gene60039.t1 AT5G38910 38.614 202 107 6 13 201 22 219 2.48e-31 114
MS.gene60039.t1 AT5G39130 35.922 206 118 6 10 201 14 219 6.70e-31 113
MS.gene60039.t1 AT3G04200 36.493 211 114 7 1 194 8 215 1.15e-30 112
MS.gene60039.t1 AT3G10080 36.310 168 105 2 37 202 53 220 6.61e-30 110
MS.gene60039.t1 AT3G62020 38.506 174 101 5 34 201 13 186 6.79e-30 109
MS.gene60039.t1 AT5G39110 36.715 207 116 6 10 201 14 220 7.34e-30 110
MS.gene60039.t1 AT4G14630 37.073 205 111 6 11 201 22 222 1.62e-29 109
MS.gene60039.t1 AT5G38930 36.683 199 117 5 12 201 23 221 1.66e-29 109
MS.gene60039.t1 AT3G10080 36.310 168 105 2 37 202 124 291 2.42e-29 110
MS.gene60039.t1 AT1G18970 36.318 201 121 5 6 201 20 218 5.79e-29 107
MS.gene60039.t1 AT5G39120 35.377 212 121 7 5 201 9 219 2.04e-28 106
MS.gene60039.t1 AT3G04150 35.227 176 106 4 38 205 50 225 2.09e-28 106
MS.gene60039.t1 AT3G04150 35.227 176 106 4 38 205 63 238 2.75e-28 106
MS.gene60039.t1 AT5G39180 37.500 176 102 4 34 201 44 219 3.40e-28 105
MS.gene60039.t1 AT5G39150 37.500 176 102 4 34 201 44 219 9.06e-28 104
MS.gene60039.t1 AT5G39190 36.364 165 98 3 44 201 33 197 9.08e-27 101
MS.gene60039.t1 AT3G04190 35.838 173 103 4 38 202 50 222 1.94e-26 101
MS.gene60039.t1 AT5G39160 35.758 165 99 3 44 201 33 197 2.22e-26 100
MS.gene60039.t1 AT5G61750 40.299 134 77 3 34 164 40 173 2.45e-26 100
MS.gene60039.t1 AT3G04180 36.416 173 102 4 38 202 50 222 4.58e-26 100
MS.gene60039.t1 AT1G74820 40.940 149 83 4 20 165 41 187 7.37e-25 97.4
MS.gene60039.t1 AT1G10460 39.716 141 80 5 34 169 41 181 5.89e-24 94.7
MS.gene60039.t1 AT3G04170 33.645 214 119 8 6 202 12 219 7.07e-24 94.7
MS.gene60039.t1 AT5G38910 31.818 198 89 5 13 201 22 182 8.15e-17 75.1

Find 32 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATACTTGATACTCTATTATT+TGG 0.194314 4.3:+86241745 MS.gene60039:CDS
AAAAGGGTTTATTGTGCTTC+CGG 0.287277 4.3:-86241410 None:intergenic
TAAAGTAGTCTCTGCAATTA+TGG 0.288055 4.3:-86241785 None:intergenic
GGTGTTATTGCAAAATTAAA+AGG 0.297840 4.3:-86241427 None:intergenic
AGAGCAGATTTCGGTATAAA+TGG 0.319908 4.3:+86241502 MS.gene60039:CDS
GGATAGCCTGAAGGGGTGTT+TGG 0.320221 4.3:-86241331 None:intergenic
TTTGTAGTTAACTCTGGTAT+TGG 0.328442 4.3:+86241673 MS.gene60039:CDS
AAAGGGTTTATTGTGCTTCC+GGG 0.366887 4.3:-86241409 None:intergenic
GATAGCCTGAAGGGGTGTTT+GGG 0.403343 4.3:-86241330 None:intergenic
GAATTACTTATCGTTGTTGA+AGG 0.404833 4.3:+86241556 MS.gene60039:CDS
ACAAAATGCAATAGTCCTTC+CGG 0.407885 4.3:-86241655 None:intergenic
CTACTCCCAAACACCCCTTC+AGG 0.414099 4.3:+86241325 MS.gene60039:CDS
AAACTGAAGGCTCAGTTTAA+CGG 0.420980 4.3:+86241829 MS.gene60039:CDS
ACATAAATCATCAGCATAAG+AGG 0.443948 4.3:-86241290 None:intergenic
ACTAGTTTACCAGGTCTTAA+CGG 0.448053 4.3:+86241460 MS.gene60039:CDS
TCTGCAGCAAGAGCAGATTT+CGG 0.459225 4.3:+86241493 MS.gene60039:CDS
GTGTTCTCTGGCTTTGTACC+CGG 0.484764 4.3:+86241391 MS.gene60039:CDS
GTATCAAGTATTTGTGTGCT+AGG 0.485474 4.3:-86241733 None:intergenic
GTGTTATTGCAAAATTAAAA+GGG 0.511452 4.3:-86241426 None:intergenic
TATTCGAAAACTGTTAAACC+TGG 0.530998 4.3:+86241616 MS.gene60039:CDS
GTTGAAGGTCAAATTACTGC+CGG 0.534035 4.3:+86241571 MS.gene60039:CDS
GACTTGCATGGATAGCCTGA+AGG 0.536376 4.3:-86241340 None:intergenic
GCATTTGTCACTAGTTTACC+AGG 0.540832 4.3:+86241451 MS.gene60039:CDS
TTACCAGGTCTTAACGGACT+TGG 0.542899 4.3:+86241466 MS.gene60039:CDS
AAGGCTCAGTTTAACGGCAC+TGG 0.546212 4.3:+86241835 MS.gene60039:CDS
ACTTGCATGGATAGCCTGAA+GGG 0.557716 4.3:-86241339 None:intergenic
ACATTTGTTTCTGACTTGCA+TGG 0.591769 4.3:-86241352 None:intergenic
GGTAAACTAGTGACAAATGC+AGG 0.592955 4.3:-86241448 None:intergenic
GTTGCATCATGAGTGTGCAC+TGG 0.602414 4.3:-86241532 None:intergenic
GTCACAGATTCTGAAACTGA+AGG 0.635927 4.3:+86241816 MS.gene60039:CDS
ATACCAAGTCCGTTAAGACC+TGG 0.647866 4.3:-86241469 None:intergenic
CTTGCATGGATAGCCTGAAG+GGG 0.690334 4.3:-86241338 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATACTTGATACTCTATTATT+TGG + chr4.3:86241745-86241764 MS.gene60039:CDS 20.0%
!!! GTGTTATTGCAAAATTAAAA+GGG - chr4.3:86241429-86241448 None:intergenic 20.0%
!! TTTTGTTCGTGTAATAAATC+CGG - chr4.3:86241593-86241612 None:intergenic 25.0%
!!! GGTGTTATTGCAAAATTAAA+AGG - chr4.3:86241430-86241449 None:intergenic 25.0%
ACATAAATCATCAGCATAAG+AGG - chr4.3:86241293-86241312 None:intergenic 30.0%
GAATTACTTATCGTTGTTGA+AGG + chr4.3:86241556-86241575 MS.gene60039:CDS 30.0%
TAAAGTAGTCTCTGCAATTA+TGG - chr4.3:86241788-86241807 None:intergenic 30.0%
TATTCGAAAACTGTTAAACC+TGG + chr4.3:86241616-86241635 MS.gene60039:CDS 30.0%
TTTGTAGTTAACTCTGGTAT+TGG + chr4.3:86241673-86241692 MS.gene60039:CDS 30.0%
! TGGTGATCTTATAGTTTTTC+CGG + chr4.3:86241636-86241655 MS.gene60039:CDS 30.0%
! TTGCATTTTGTAGTTAACTC+TGG + chr4.3:86241667-86241686 MS.gene60039:CDS 30.0%
AAAAGGGTTTATTGTGCTTC+CGG - chr4.3:86241413-86241432 None:intergenic 35.0%
AAACTGAAGGCTCAGTTTAA+CGG + chr4.3:86241829-86241848 MS.gene60039:CDS 35.0%
ACAAAATGCAATAGTCCTTC+CGG - chr4.3:86241658-86241677 None:intergenic 35.0%
AGAGCAGATTTCGGTATAAA+TGG + chr4.3:86241502-86241521 MS.gene60039:CDS 35.0%
GGAAAAACTATAAGATCACC+AGG - chr4.3:86241637-86241656 None:intergenic 35.0%
GTATCAAGTATTTGTGTGCT+AGG - chr4.3:86241736-86241755 None:intergenic 35.0%
! ACTAGTTTACCAGGTCTTAA+CGG + chr4.3:86241460-86241479 MS.gene60039:CDS 35.0%
! GATCTTATAGTTTTTCCGGA+AGG + chr4.3:86241640-86241659 MS.gene60039:CDS 35.0%
! GTGAATGATTTTGTGTTCTC+TGG + chr4.3:86241379-86241398 MS.gene60039:CDS 35.0%
!! ACATTTGTTTCTGACTTGCA+TGG - chr4.3:86241355-86241374 None:intergenic 35.0%
AAAGGGTTTATTGTGCTTCC+GGG - chr4.3:86241412-86241431 None:intergenic 40.0%
GGTAAACTAGTGACAAATGC+AGG - chr4.3:86241451-86241470 None:intergenic 40.0%
GTTGAAGGTCAAATTACTGC+CGG + chr4.3:86241571-86241590 MS.gene60039:CDS 40.0%
! GCATTTGTCACTAGTTTACC+AGG + chr4.3:86241451-86241470 MS.gene60039:CDS 40.0%
!! GTCACAGATTCTGAAACTGA+AGG + chr4.3:86241816-86241835 MS.gene60039:CDS 40.0%
ACTTGCATGGATAGCCTGAA+GGG - chr4.3:86241342-86241361 None:intergenic 45.0%
ATACCAAGTCCGTTAAGACC+TGG - chr4.3:86241472-86241491 None:intergenic 45.0%
TCTGCAGCAAGAGCAGATTT+CGG + chr4.3:86241493-86241512 MS.gene60039:CDS 45.0%
!! TTACCAGGTCTTAACGGACT+TGG + chr4.3:86241466-86241485 MS.gene60039:CDS 45.0%
AAGGCTCAGTTTAACGGCAC+TGG + chr4.3:86241835-86241854 MS.gene60039:CDS 50.0%
CTTGCATGGATAGCCTGAAG+GGG - chr4.3:86241341-86241360 None:intergenic 50.0%
GACTTGCATGGATAGCCTGA+AGG - chr4.3:86241343-86241362 None:intergenic 50.0%
GTTGCATCATGAGTGTGCAC+TGG - chr4.3:86241535-86241554 None:intergenic 50.0%
!! GATAGCCTGAAGGGGTGTTT+GGG - chr4.3:86241333-86241352 None:intergenic 50.0%
!! GTGTTCTCTGGCTTTGTACC+CGG + chr4.3:86241391-86241410 MS.gene60039:CDS 50.0%
CTACTCCCAAACACCCCTTC+AGG + chr4.3:86241325-86241344 MS.gene60039:CDS 55.0%
!! GGATAGCCTGAAGGGGTGTT+TGG - chr4.3:86241334-86241353 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 86241244 86241861 86241244 ID=MS.gene60039
chr4.3 mRNA 86241244 86241861 86241244 ID=MS.gene60039.t1;Parent=MS.gene60039
chr4.3 exon 86241244 86241861 86241244 ID=MS.gene60039.t1.exon1;Parent=MS.gene60039.t1
chr4.3 CDS 86241244 86241861 86241244 ID=cds.MS.gene60039.t1;Parent=MS.gene60039.t1
Gene Sequence

>MS.gene60039

ATGATTCACACTATTCTTTTCTTTTTTGCTCTTCTCTCATCATATACCTCTTATGCTGATGATTTATGTGTAGCAGACTTACTACTCCCAAACACCCCTTCAGGCTATCCATGCAAGTCAGAAACAAATGTAACAGTGAATGATTTTGTGTTCTCTGGCTTTGTACCCGGAAGCACAATAAACCCTTTTAATTTTGCAATAACACCTGCATTTGTCACTAGTTTACCAGGTCTTAACGGACTTGGTATCTCTGCAGCAAGAGCAGATTTCGGTATAAATGGACGTGTTCCAGTGCACACTCATGATGCAACTGAATTACTTATCGTTGTTGAAGGTCAAATTACTGCCGGATTTATTACACGAACAAAAGTTTATTCGAAAACTGTTAAACCTGGTGATCTTATAGTTTTTCCGGAAGGACTATTGCATTTTGTAGTTAACTCTGGTATTGGAAAAGCTGTTGCTTTTGTTGCTTTTAGTAGCTCAAATCCTAGCACACAAATACTTGATACTCTATTATTTGGCAACAATTTATCAACTTCCATAATTGCAGAGACTACTTTACTTGATGTGTCACAGATTCTGAAACTGAAGGCTCAGTTTAACGGCACTGGCTAG

Protein sequence

>MS.gene60039.t1

MIHTILFFFALLSSYTSYADDLCVADLLLPNTPSGYPCKSETNVTVNDFVFSGFVPGSTINPFNFAITPAFVTSLPGLNGLGISAARADFGINGRVPVHTHDATELLIVVEGQITAGFITRTKVYSKTVKPGDLIVFPEGLLHFVVNSGIGKAVAFVAFSSSNPSTQILDTLLFGNNLSTSIIAETTLLDVSQILKLKAQFNGTG