Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054799.t1 | XP_003602846.1 | 92.2 | 192 | 9 | 2 | 1 | 192 | 1 | 186 | 2.40E-55 | 225.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054799.t1 | Q41001 | 83.6 | 122 | 17 | 3 | 1 | 120 | 1 | 121 | 5.7e-50 | 198.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054799.t1 | B7FHG1 | 92.7 | 192 | 9 | 2 | 1 | 192 | 1 | 187 | 1.7e-55 | 225.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054799 | MS.gene20057 | 0.827504 | 1.51E-54 | -1.69E-46 |
| MS.gene054799 | MS.gene22440 | 0.81865 | 1.76E-52 | -1.69E-46 |
| MS.gene054799 | MS.gene22779 | 0.804098 | 2.54E-49 | -1.69E-46 |
| MS.gene054799 | MS.gene31312 | 0.800756 | 1.24E-48 | -1.69E-46 |
| MS.gene054799 | MS.gene38580 | 0.953774 | 1.30E-111 | -1.69E-46 |
| MS.gene054799 | MS.gene38581 | 0.997176 | 4.18E-238 | -1.69E-46 |
| MS.gene054799 | MS.gene38601 | 0.844982 | 5.39E-59 | -1.69E-46 |
| MS.gene054799 | MS.gene60039 | 0.811011 | 8.67E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene47150 | MS.gene054799 | PPI |
| MS.gene55509 | MS.gene054799 | PPI |
| MS.gene074086 | MS.gene054799 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054799.t1 | MTR_3g099580 | 87.500 | 192 | 18 | 2 | 1 | 192 | 1 | 186 | 9.85e-94 | 271 |
| MS.gene054799.t1 | MTR_3g099570 | 80.208 | 192 | 30 | 3 | 1 | 192 | 1 | 184 | 3.69e-92 | 267 |
| MS.gene054799.t1 | MTR_3g099680 | 61.818 | 220 | 41 | 4 | 7 | 188 | 6 | 220 | 6.79e-71 | 214 |
| MS.gene054799.t1 | MTR_3g099980 | 62.385 | 218 | 41 | 4 | 7 | 188 | 6 | 218 | 3.65e-70 | 213 |
| MS.gene054799.t1 | MTR_3g099540 | 67.717 | 127 | 41 | 0 | 1 | 127 | 1 | 127 | 9.32e-58 | 178 |
| MS.gene054799.t1 | MTR_8g007035 | 56.098 | 123 | 50 | 3 | 5 | 124 | 2 | 123 | 8.30e-40 | 134 |
| MS.gene054799.t1 | MTR_8g007020 | 46.486 | 185 | 89 | 6 | 7 | 186 | 4 | 183 | 8.73e-40 | 134 |
| MS.gene054799.t1 | MTR_1g090190 | 35.928 | 167 | 100 | 2 | 24 | 190 | 20 | 179 | 2.76e-32 | 114 |
| MS.gene054799.t1 | MTR_5g006040 | 41.290 | 155 | 79 | 8 | 13 | 161 | 10 | 158 | 5.42e-29 | 106 |
| MS.gene054799.t1 | MTR_2g088990 | 40.816 | 147 | 79 | 4 | 13 | 153 | 12 | 156 | 4.60e-28 | 105 |
| MS.gene054799.t1 | MTR_4g114870 | 50.833 | 120 | 51 | 4 | 25 | 138 | 27 | 144 | 6.56e-28 | 107 |
| MS.gene054799.t1 | MTR_4g114870 | 50.000 | 100 | 45 | 2 | 27 | 121 | 191 | 290 | 2.21e-25 | 101 |
| MS.gene054799.t1 | MTR_6g080660 | 48.000 | 100 | 52 | 0 | 27 | 126 | 28 | 127 | 1.22e-27 | 103 |
| MS.gene054799.t1 | MTR_1g112700 | 34.694 | 147 | 85 | 4 | 3 | 138 | 7 | 153 | 1.22e-25 | 97.8 |
| MS.gene054799.t1 | MTR_0334s0010 | 39.516 | 124 | 72 | 1 | 3 | 123 | 4 | 127 | 2.28e-25 | 97.8 |
| MS.gene054799.t1 | MTR_1g104800 | 38.710 | 124 | 71 | 5 | 1 | 121 | 1 | 122 | 3.06e-24 | 94.4 |
| MS.gene054799.t1 | MTR_6g023760 | 44.118 | 102 | 56 | 1 | 21 | 121 | 20 | 121 | 1.42e-23 | 92.4 |
| MS.gene054799.t1 | MTR_4g067200 | 39.552 | 134 | 74 | 4 | 3 | 129 | 4 | 137 | 2.60e-23 | 91.7 |
| MS.gene054799.t1 | MTR_4g066110 | 39.552 | 134 | 74 | 4 | 3 | 129 | 4 | 137 | 2.60e-23 | 91.7 |
| MS.gene054799.t1 | MTR_7g086140 | 41.860 | 129 | 66 | 4 | 1 | 124 | 1 | 125 | 4.61e-23 | 90.5 |
| MS.gene054799.t1 | MTR_7g086190 | 41.860 | 129 | 66 | 4 | 1 | 124 | 1 | 125 | 4.61e-23 | 90.5 |
| MS.gene054799.t1 | MTR_7g086160 | 38.889 | 126 | 75 | 2 | 1 | 124 | 1 | 126 | 5.69e-22 | 87.8 |
| MS.gene054799.t1 | MTR_6g083240 | 36.364 | 165 | 95 | 5 | 1 | 158 | 1 | 162 | 1.38e-21 | 87.0 |
| MS.gene054799.t1 | MTR_1g105130 | 39.474 | 114 | 64 | 3 | 13 | 121 | 8 | 121 | 1.67e-21 | 86.3 |
| MS.gene054799.t1 | MTR_1g105120 | 39.474 | 114 | 64 | 3 | 13 | 121 | 8 | 121 | 1.67e-21 | 86.3 |
| MS.gene054799.t1 | MTR_8g099220 | 41.748 | 103 | 56 | 2 | 24 | 122 | 26 | 128 | 5.33e-21 | 84.3 |
| MS.gene054799.t1 | MTR_7g086090 | 38.400 | 125 | 71 | 3 | 1 | 122 | 1 | 122 | 8.16e-20 | 84.0 |
| MS.gene054799.t1 | MTR_7g086220 | 36.434 | 129 | 72 | 4 | 1 | 124 | 1 | 124 | 8.97e-20 | 82.0 |
| MS.gene054799.t1 | MTR_7g086230 | 37.398 | 123 | 75 | 2 | 1 | 122 | 1 | 122 | 2.00e-19 | 81.6 |
| MS.gene054799.t1 | MTR_2g025580 | 36.029 | 136 | 83 | 3 | 16 | 147 | 16 | 151 | 3.13e-19 | 80.9 |
| MS.gene054799.t1 | MTR_7g086100 | 38.525 | 122 | 73 | 2 | 1 | 121 | 1 | 121 | 3.66e-19 | 82.0 |
| MS.gene054799.t1 | MTR_4g081100 | 31.731 | 208 | 115 | 4 | 10 | 190 | 15 | 222 | 3.68e-19 | 82.0 |
| MS.gene054799.t1 | MTR_2g043730 | 43.000 | 100 | 53 | 3 | 22 | 120 | 27 | 123 | 1.27e-18 | 78.2 |
| MS.gene054799.t1 | MTR_8g463180 | 35.075 | 134 | 79 | 4 | 12 | 141 | 8 | 137 | 1.93e-18 | 81.3 |
| MS.gene054799.t1 | MTR_1g090420 | 31.429 | 175 | 100 | 5 | 1 | 155 | 2 | 176 | 2.40e-18 | 79.7 |
| MS.gene054799.t1 | MTR_7g086280 | 36.585 | 123 | 76 | 2 | 1 | 122 | 1 | 122 | 2.95e-18 | 78.6 |
| MS.gene054799.t1 | MTR_2g083250 | 42.157 | 102 | 52 | 2 | 29 | 123 | 28 | 129 | 4.55e-18 | 79.7 |
| MS.gene054799.t1 | MTR_2g090580 | 37.410 | 139 | 76 | 4 | 29 | 159 | 29 | 164 | 5.20e-18 | 78.6 |
| MS.gene054799.t1 | MTR_4g077787 | 28.485 | 165 | 108 | 3 | 2 | 160 | 7 | 167 | 2.83e-17 | 75.9 |
| MS.gene054799.t1 | MTR_6g013200 | 29.143 | 175 | 110 | 5 | 1 | 171 | 8 | 172 | 3.77e-17 | 75.5 |
| MS.gene054799.t1 | MTR_3g089035 | 43.299 | 97 | 51 | 3 | 24 | 119 | 30 | 123 | 4.41e-17 | 73.9 |
| MS.gene054799.t1 | MTR_4g111640 | 32.716 | 162 | 92 | 6 | 13 | 168 | 24 | 174 | 5.00e-17 | 75.5 |
| MS.gene054799.t1 | MTR_6g013420 | 38.655 | 119 | 66 | 5 | 8 | 122 | 20 | 135 | 6.43e-17 | 75.1 |
| MS.gene054799.t1 | MTR_2g101300 | 34.641 | 153 | 88 | 4 | 7 | 149 | 3 | 153 | 7.07e-17 | 75.1 |
| MS.gene054799.t1 | MTR_4g007250 | 38.835 | 103 | 61 | 2 | 21 | 122 | 23 | 124 | 2.22e-16 | 73.6 |
| MS.gene054799.t1 | MTR_2g090575 | 36.364 | 121 | 70 | 3 | 8 | 121 | 2 | 122 | 7.33e-16 | 74.7 |
| MS.gene054799.t1 | MTR_6g022170 | 37.864 | 103 | 58 | 3 | 24 | 121 | 31 | 132 | 9.37e-16 | 71.6 |
| MS.gene054799.t1 | MTR_4g078410 | 32.258 | 155 | 100 | 4 | 29 | 180 | 27 | 179 | 4.37e-15 | 70.5 |
| MS.gene054799.t1 | MTR_3g105930 | 33.333 | 120 | 76 | 2 | 42 | 160 | 47 | 163 | 9.79e-15 | 69.3 |
| MS.gene054799.t1 | MTR_8g094990 | 29.897 | 194 | 108 | 6 | 8 | 174 | 2 | 194 | 1.08e-14 | 70.9 |
| MS.gene054799.t1 | MTR_4g112310 | 32.812 | 128 | 81 | 3 | 41 | 168 | 2 | 124 | 1.63e-14 | 67.4 |
| MS.gene054799.t1 | MTR_2g043790 | 41.414 | 99 | 54 | 3 | 22 | 119 | 2 | 97 | 1.81e-14 | 66.6 |
| MS.gene054799.t1 | MTR_1g014120 | 38.614 | 101 | 58 | 2 | 42 | 138 | 45 | 145 | 3.59e-14 | 67.8 |
| MS.gene054799.t1 | MTR_4g130780 | 30.714 | 140 | 91 | 3 | 1 | 134 | 3 | 142 | 5.81e-14 | 67.4 |
| MS.gene054799.t1 | MTR_4g124280 | 30.769 | 143 | 85 | 3 | 24 | 161 | 22 | 155 | 6.83e-14 | 68.2 |
| MS.gene054799.t1 | MTR_8g095020 | 34.228 | 149 | 78 | 5 | 28 | 162 | 26 | 168 | 8.84e-14 | 68.6 |
| MS.gene054799.t1 | MTR_4g114830 | 37.037 | 108 | 59 | 5 | 18 | 116 | 18 | 125 | 1.15e-13 | 65.9 |
| MS.gene054799.t1 | MTR_4g130800 | 34.375 | 128 | 76 | 5 | 1 | 121 | 3 | 129 | 1.27e-13 | 67.8 |
| MS.gene054799.t1 | MTR_8g086360 | 36.029 | 136 | 75 | 6 | 12 | 138 | 5 | 137 | 1.72e-13 | 66.2 |
| MS.gene054799.t1 | MTR_4g130800 | 40.506 | 79 | 46 | 1 | 44 | 121 | 50 | 128 | 5.24e-13 | 65.9 |
| MS.gene054799.t1 | MTR_8g088830 | 39.000 | 100 | 52 | 5 | 24 | 119 | 25 | 119 | 5.57e-13 | 63.2 |
| MS.gene054799.t1 | MTR_8g095013 | 30.682 | 176 | 101 | 6 | 8 | 166 | 2 | 173 | 9.93e-13 | 65.5 |
| MS.gene054799.t1 | MTR_5g088170 | 33.929 | 112 | 68 | 4 | 30 | 138 | 20 | 128 | 1.98e-11 | 59.7 |
| MS.gene054799.t1 | MTR_5g088180 | 33.929 | 112 | 68 | 4 | 30 | 138 | 33 | 141 | 2.48e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054799.t1 | AT1G72230 | 47.594 | 187 | 84 | 3 | 3 | 187 | 1 | 175 | 1.15e-49 | 159 |
| MS.gene054799.t1 | AT1G22480 | 64.130 | 92 | 33 | 0 | 35 | 126 | 27 | 118 | 4.20e-36 | 124 |
| MS.gene054799.t1 | AT2G32300 | 43.651 | 126 | 70 | 1 | 4 | 128 | 3 | 128 | 7.67e-30 | 110 |
| MS.gene054799.t1 | AT2G32300 | 43.651 | 126 | 70 | 1 | 4 | 128 | 33 | 158 | 2.82e-29 | 110 |
| MS.gene054799.t1 | AT3G60270 | 47.115 | 104 | 55 | 0 | 23 | 126 | 22 | 125 | 5.23e-28 | 104 |
| MS.gene054799.t1 | AT2G26720 | 38.182 | 165 | 98 | 4 | 25 | 186 | 27 | 190 | 5.60e-27 | 102 |
| MS.gene054799.t1 | AT3G27200 | 41.129 | 124 | 67 | 3 | 1 | 121 | 1 | 121 | 1.82e-26 | 99.8 |
| MS.gene054799.t1 | AT5G07475 | 36.538 | 156 | 82 | 3 | 24 | 179 | 27 | 165 | 3.50e-26 | 99.8 |
| MS.gene054799.t1 | AT2G31050 | 45.217 | 115 | 58 | 3 | 25 | 137 | 27 | 138 | 2.83e-25 | 97.4 |
| MS.gene054799.t1 | AT2G44790 | 50.526 | 95 | 45 | 1 | 35 | 127 | 36 | 130 | 3.83e-25 | 97.1 |
| MS.gene054799.t1 | AT5G26330 | 44.554 | 101 | 53 | 2 | 24 | 121 | 21 | 121 | 9.57e-24 | 93.2 |
| MS.gene054799.t1 | AT3G17675 | 38.095 | 105 | 64 | 1 | 18 | 121 | 23 | 127 | 3.05e-21 | 85.1 |
| MS.gene054799.t1 | AT3G60280 | 42.157 | 102 | 59 | 0 | 23 | 124 | 20 | 121 | 1.70e-20 | 85.5 |
| MS.gene054799.t1 | AT5G53870 | 33.566 | 143 | 79 | 3 | 7 | 138 | 7 | 144 | 4.20e-18 | 81.3 |
| MS.gene054799.t1 | AT5G53870 | 33.566 | 143 | 79 | 3 | 7 | 138 | 7 | 144 | 6.58e-18 | 80.5 |
| MS.gene054799.t1 | AT5G20230 | 36.628 | 172 | 90 | 5 | 27 | 179 | 25 | 196 | 1.13e-17 | 77.4 |
| MS.gene054799.t1 | AT4G31840 | 33.803 | 142 | 86 | 4 | 3 | 136 | 1 | 142 | 4.29e-17 | 75.5 |
| MS.gene054799.t1 | AT2G25060 | 37.719 | 114 | 65 | 3 | 28 | 135 | 32 | 145 | 8.47e-17 | 74.7 |
| MS.gene054799.t1 | AT3G18590 | 34.646 | 127 | 76 | 2 | 1 | 121 | 1 | 126 | 1.42e-16 | 74.3 |
| MS.gene054799.t1 | AT4G28365 | 35.965 | 114 | 69 | 3 | 29 | 138 | 31 | 144 | 1.13e-15 | 72.0 |
| MS.gene054799.t1 | AT1G64640 | 37.705 | 122 | 69 | 3 | 24 | 138 | 30 | 151 | 1.16e-15 | 72.0 |
| MS.gene054799.t1 | AT5G15350 | 36.667 | 120 | 71 | 3 | 8 | 122 | 7 | 126 | 2.94e-15 | 70.5 |
| MS.gene054799.t1 | AT4G27520 | 34.722 | 144 | 83 | 4 | 10 | 142 | 9 | 152 | 3.78e-15 | 72.8 |
| MS.gene054799.t1 | AT5G57920 | 37.113 | 97 | 59 | 2 | 42 | 137 | 43 | 138 | 5.19e-15 | 70.1 |
| MS.gene054799.t1 | AT5G57920 | 37.113 | 97 | 59 | 2 | 42 | 137 | 75 | 170 | 5.63e-15 | 70.5 |
| MS.gene054799.t1 | AT5G57920 | 37.113 | 97 | 59 | 2 | 42 | 137 | 42 | 137 | 8.75e-15 | 69.3 |
| MS.gene054799.t1 | AT3G53330 | 32.540 | 126 | 78 | 4 | 23 | 144 | 184 | 306 | 1.75e-14 | 70.5 |
| MS.gene054799.t1 | AT4G30590 | 33.673 | 98 | 64 | 1 | 42 | 138 | 48 | 145 | 4.93e-14 | 67.8 |
| MS.gene054799.t1 | AT4G12880 | 34.167 | 120 | 72 | 5 | 7 | 122 | 10 | 126 | 8.44e-14 | 65.9 |
| MS.gene054799.t1 | AT1G45063 | 36.207 | 116 | 63 | 5 | 26 | 135 | 148 | 258 | 1.25e-13 | 67.8 |
| MS.gene054799.t1 | AT1G45063 | 39.216 | 102 | 54 | 4 | 26 | 121 | 148 | 247 | 1.42e-13 | 68.6 |
| MS.gene054799.t1 | AT5G14345 | 30.469 | 128 | 81 | 3 | 13 | 133 | 2 | 128 | 1.69e-13 | 65.1 |
| MS.gene054799.t1 | AT3G20570 | 34.286 | 175 | 102 | 6 | 9 | 173 | 15 | 186 | 2.35e-13 | 66.2 |
| MS.gene054799.t1 | AT1G48940 | 33.333 | 120 | 73 | 3 | 8 | 121 | 7 | 125 | 5.82e-13 | 64.3 |
| MS.gene054799.t1 | AT4G32490 | 30.841 | 107 | 71 | 2 | 29 | 132 | 33 | 139 | 1.06e-12 | 64.7 |
| MS.gene054799.t1 | AT2G02850 | 39.000 | 100 | 51 | 4 | 24 | 119 | 33 | 126 | 2.05e-12 | 62.0 |
| MS.gene054799.t1 | AT1G79800 | 33.333 | 117 | 67 | 3 | 29 | 135 | 36 | 151 | 2.23e-12 | 63.2 |
| MS.gene054799.t1 | AT2G23990 | 33.684 | 95 | 61 | 2 | 42 | 134 | 47 | 141 | 3.11e-12 | 63.2 |
| MS.gene054799.t1 | AT5G25090 | 32.258 | 124 | 77 | 3 | 7 | 125 | 9 | 130 | 1.96e-11 | 60.5 |
| MS.gene054799.t1 | AT4G01380 | 31.193 | 109 | 65 | 4 | 33 | 133 | 78 | 184 | 5.69e-11 | 59.7 |
| MS.gene054799.t1 | AT3G01070 | 38.462 | 117 | 66 | 3 | 9 | 122 | 12 | 125 | 6.15e-11 | 58.9 |
Find 55 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAAGGAGTGGTGGTGGTGG+GGG | 0.228476 | 3.3:+81976043 | None:intergenic |
| CTTCTTCTTAGCAATTAATA+TGG | 0.256926 | 3.3:-81976644 | MS.gene054799:CDS |
| GGTGTGGTGGTGGTTGCAGC+AGG | 0.286767 | 3.3:+81976065 | None:intergenic |
| TGGAAGGAGTGGTGGTGGTG+GGG | 0.303181 | 3.3:+81976042 | None:intergenic |
| GAAGGAGTGGTGGTGGTGGG+GGG | 0.308049 | 3.3:+81976044 | None:intergenic |
| TGTGGAAGGAGTGGTGGTGG+TGG | 0.315589 | 3.3:+81976040 | None:intergenic |
| GATCTTGATCAGTTATATAT+TGG | 0.317167 | 3.3:-81975955 | MS.gene054799:CDS |
| TGTGGTGGTGGTTGCAGCAG+GGG | 0.329834 | 3.3:+81976067 | None:intergenic |
| GAAACTTTCTGTTAAGGTTA+AGG | 0.337776 | 3.3:-81976162 | MS.gene054799:CDS |
| TACTTCATATGTGCTGTTCC+TGG | 0.349993 | 3.3:-81976203 | MS.gene054799:CDS |
| GTGGAAGGAGTGGTGGTGGT+GGG | 0.351920 | 3.3:+81976041 | None:intergenic |
| GATAAATCAGGTTGGGCAAT+TGG | 0.365257 | 3.3:-81976586 | MS.gene054799:CDS |
| GACTATAAATCATGCACAAC+TGG | 0.368543 | 3.3:-81976290 | MS.gene054799:CDS |
| ACTATGACAGTGTTCAACTA+TGG | 0.385966 | 3.3:-81976347 | MS.gene054799:intron |
| TGGCATGAAACTTTCTGTTA+AGG | 0.430118 | 3.3:-81976168 | MS.gene054799:CDS |
| TCCTGCTTTCTTGAGAGGGA+TGG | 0.445617 | 3.3:+81976232 | None:intergenic |
| TCTGCAACACCATCACCATC+AGG | 0.461815 | 3.3:-81976113 | MS.gene054799:CDS |
| GTGTGGTGGTGGTTGCAGCA+GGG | 0.463658 | 3.3:+81976066 | None:intergenic |
| ATGCCACCTGTGCAATGTCC+AGG | 0.481287 | 3.3:+81976185 | None:intergenic |
| GTGTTCCTGCTTTCTTGAGA+GGG | 0.482705 | 3.3:+81976228 | None:intergenic |
| TGTGTTCCTGCTTTCTTGAG+AGG | 0.493116 | 3.3:+81976227 | None:intergenic |
| TTTCTTGAGAGGGATGGTTG+TGG | 0.493786 | 3.3:+81976238 | None:intergenic |
| CACACCGTTGGAGATAAATC+AGG | 0.494660 | 3.3:-81976598 | MS.gene054799:CDS |
| GGCTAGTGACAAAACATTTG+CGG | 0.495560 | 3.3:-81976545 | MS.gene054799:CDS |
| TTTGCGGTTGGTGACAGTCT+TGG | 0.500707 | 3.3:-81976529 | MS.gene054799:intron |
| CGTTGGAGATAAATCAGGTT+GGG | 0.514147 | 3.3:-81976593 | MS.gene054799:CDS |
| GGTTGCAGCAGGGGTGCCAT+CGG | 0.524569 | 3.3:+81976076 | None:intergenic |
| AAGAAGCCTAAAATCAAAGA+AGG | 0.528032 | 3.3:+81976661 | None:intergenic |
| CTTGCAACTGTCCACACCGT+TGG | 0.529565 | 3.3:-81976610 | MS.gene054799:CDS |
| TTGTGTGGAAGGAGTGGTGG+TGG | 0.529906 | 3.3:+81976037 | None:intergenic |
| GCAGCAGGGGTGCCATCGGA+AGG | 0.553762 | 3.3:+81976080 | None:intergenic |
| ACCATCCCTCTCAAGAAAGC+AGG | 0.564418 | 3.3:-81976233 | MS.gene054799:CDS |
| GAACCTTTCCCTGATGGTGA+TGG | 0.566964 | 3.3:+81976104 | None:intergenic |
| GTTCCTGGACATTGCACAGG+TGG | 0.574513 | 3.3:-81976188 | MS.gene054799:CDS |
| GAAGGTGAACCTTTCCCTGA+TGG | 0.578557 | 3.3:+81976098 | None:intergenic |
| CCAACCTGATTTATCTCCAA+CGG | 0.588831 | 3.3:+81976594 | None:intergenic |
| GTGTGGACAGTTGCAAGAGT+TGG | 0.591396 | 3.3:+81976616 | None:intergenic |
| AGTGACAAAACATTTGCGGT+TGG | 0.594495 | 3.3:-81976541 | MS.gene054799:CDS |
| GCTGTTCCTGGACATTGCAC+AGG | 0.594776 | 3.3:-81976191 | MS.gene054799:CDS |
| CTTTAACTTCATCCACTGTG+TGG | 0.606822 | 3.3:+81976318 | None:intergenic |
| ACAGTGTTCAACTATGGTGC+TGG | 0.611527 | 3.3:-81976341 | MS.gene054799:intron |
| GATGGTGATGGTGTTGCAGA+AGG | 0.612608 | 3.3:+81976116 | None:intergenic |
| TTGGTTCTGATTATAACACA+TGG | 0.617869 | 3.3:-81976567 | MS.gene054799:CDS |
| AGCTGAAGATGCACTTTGTG+TGG | 0.619201 | 3.3:+81976022 | None:intergenic |
| CTGCAACACCATCACCATCA+GGG | 0.628504 | 3.3:-81976112 | MS.gene054799:CDS |
| GAAGATGCACTTTGTGTGGA+AGG | 0.630949 | 3.3:+81976026 | None:intergenic |
| TGCACTTTGTGTGGAAGGAG+TGG | 0.632667 | 3.3:+81976031 | None:intergenic |
| CCGTTGGAGATAAATCAGGT+TGG | 0.633245 | 3.3:-81976594 | MS.gene054799:CDS |
| GGGAAAGGTTCACCTTCCGA+TGG | 0.642087 | 3.3:-81976092 | MS.gene054799:CDS |
| ACTTTGTGTGGAAGGAGTGG+TGG | 0.644852 | 3.3:+81976034 | None:intergenic |
| ACACCATCACCATCAGGGAA+AGG | 0.655836 | 3.3:-81976107 | MS.gene054799:CDS |
| TGGTTCTGATTATAACACAT+GGG | 0.658992 | 3.3:-81976566 | MS.gene054799:CDS |
| CTGATTTATCTCCAACGGTG+TGG | 0.663800 | 3.3:+81976599 | None:intergenic |
| TCAATTAGTACAGACAGTAG+TGG | 0.678569 | 3.3:-81976263 | MS.gene054799:CDS |
| CTATGGTGCTGGCCACACAG+TGG | 0.772990 | 3.3:-81976330 | MS.gene054799:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATATTAGATTTGTAAATTG+TGG | + | chr3.3:81976250-81976269 | None:intergenic | 15.0% |
| ! | CTTCTTCTTAGCAATTAATA+TGG | - | chr3.3:81975982-81976001 | MS.gene054799:CDS | 25.0% |
| ! | GATCTTGATCAGTTATATAT+TGG | - | chr3.3:81976671-81976690 | MS.gene054799:CDS | 25.0% |
| ! | GTGAAAAATAAAGCAACAAT+TGG | + | chr3.3:81976642-81976661 | None:intergenic | 25.0% |
| !!! | CTTTATTTTTCACTGTTTCA+TGG | - | chr3.3:81976649-81976668 | MS.gene054799:CDS | 25.0% |
| !!! | TCTAATCCTTCTTTGATTTT+AGG | - | chr3.3:81975959-81975978 | MS.gene054799:CDS | 25.0% |
| AAGAAGCCTAAAATCAAAGA+AGG | + | chr3.3:81975968-81975987 | None:intergenic | 30.0% | |
| GAAACTTTCTGTTAAGGTTA+AGG | - | chr3.3:81976464-81976483 | MS.gene054799:intron | 30.0% | |
| !! | TGGTTCTGATTATAACACAT+GGG | - | chr3.3:81976060-81976079 | MS.gene054799:CDS | 30.0% |
| !! | TTGGTTCTGATTATAACACA+TGG | - | chr3.3:81976059-81976078 | MS.gene054799:CDS | 30.0% |
| ACTATGACAGTGTTCAACTA+TGG | - | chr3.3:81976279-81976298 | MS.gene054799:CDS | 35.0% | |
| GACTATAAATCATGCACAAC+TGG | - | chr3.3:81976336-81976355 | MS.gene054799:CDS | 35.0% | |
| TCAATTAGTACAGACAGTAG+TGG | - | chr3.3:81976363-81976382 | MS.gene054799:intron | 35.0% | |
| TGGCATGAAACTTTCTGTTA+AGG | - | chr3.3:81976458-81976477 | MS.gene054799:intron | 35.0% | |
| AGTGACAAAACATTTGCGGT+TGG | - | chr3.3:81976085-81976104 | MS.gene054799:CDS | 40.0% | |
| CCAACCTGATTTATCTCCAA+CGG | + | chr3.3:81976035-81976054 | None:intergenic | 40.0% | |
| CGTTGGAGATAAATCAGGTT+GGG | - | chr3.3:81976033-81976052 | MS.gene054799:CDS | 40.0% | |
| GGCTAGTGACAAAACATTTG+CGG | - | chr3.3:81976081-81976100 | MS.gene054799:CDS | 40.0% | |
| ! | CTTTAACTTCATCCACTGTG+TGG | + | chr3.3:81976311-81976330 | None:intergenic | 40.0% |
| ! | TACTTCATATGTGCTGTTCC+TGG | - | chr3.3:81976423-81976442 | MS.gene054799:intron | 40.0% |
| !! | GATAAATCAGGTTGGGCAAT+TGG | - | chr3.3:81976040-81976059 | MS.gene054799:CDS | 40.0% |
| ACAGTGTTCAACTATGGTGC+TGG | - | chr3.3:81976285-81976304 | MS.gene054799:CDS | 45.0% | |
| AGCTGAAGATGCACTTTGTG+TGG | + | chr3.3:81976607-81976626 | None:intergenic | 45.0% | |
| CACACCGTTGGAGATAAATC+AGG | - | chr3.3:81976028-81976047 | MS.gene054799:CDS | 45.0% | |
| CCGTTGGAGATAAATCAGGT+TGG | - | chr3.3:81976032-81976051 | MS.gene054799:CDS | 45.0% | |
| CTGATTTATCTCCAACGGTG+TGG | + | chr3.3:81976030-81976049 | None:intergenic | 45.0% | |
| GAAGATGCACTTTGTGTGGA+AGG | + | chr3.3:81976603-81976622 | None:intergenic | 45.0% | |
| GTGTTCCTGCTTTCTTGAGA+GGG | + | chr3.3:81976401-81976420 | None:intergenic | 45.0% | |
| TGTGTTCCTGCTTTCTTGAG+AGG | + | chr3.3:81976402-81976421 | None:intergenic | 45.0% | |
| TTTCTTGAGAGGGATGGTTG+TGG | + | chr3.3:81976391-81976410 | None:intergenic | 45.0% | |
| ACACCATCACCATCAGGGAA+AGG | - | chr3.3:81976519-81976538 | MS.gene054799:intron | 50.0% | |
| ACCATCCCTCTCAAGAAAGC+AGG | - | chr3.3:81976393-81976412 | MS.gene054799:intron | 50.0% | |
| ACTTTGTGTGGAAGGAGTGG+TGG | + | chr3.3:81976595-81976614 | None:intergenic | 50.0% | |
| CTGCAACACCATCACCATCA+GGG | - | chr3.3:81976514-81976533 | MS.gene054799:intron | 50.0% | |
| GAAGGTGAACCTTTCCCTGA+TGG | + | chr3.3:81976531-81976550 | None:intergenic | 50.0% | |
| GTGTGGACAGTTGCAAGAGT+TGG | + | chr3.3:81976013-81976032 | None:intergenic | 50.0% | |
| TCCTGCTTTCTTGAGAGGGA+TGG | + | chr3.3:81976397-81976416 | None:intergenic | 50.0% | |
| TCTGCAACACCATCACCATC+AGG | - | chr3.3:81976513-81976532 | MS.gene054799:intron | 50.0% | |
| TGCACTTTGTGTGGAAGGAG+TGG | + | chr3.3:81976598-81976617 | None:intergenic | 50.0% | |
| ! | GAACCTTTCCCTGATGGTGA+TGG | + | chr3.3:81976525-81976544 | None:intergenic | 50.0% |
| !! | GATGGTGATGGTGTTGCAGA+AGG | + | chr3.3:81976513-81976532 | None:intergenic | 50.0% |
| !! | TTTGCGGTTGGTGACAGTCT+TGG | - | chr3.3:81976097-81976116 | MS.gene054799:CDS | 50.0% |
| ATGCCACCTGTGCAATGTCC+AGG | + | chr3.3:81976444-81976463 | None:intergenic | 55.0% | |
| CTTGCAACTGTCCACACCGT+TGG | - | chr3.3:81976016-81976035 | MS.gene054799:CDS | 55.0% | |
| GCTGTTCCTGGACATTGCAC+AGG | - | chr3.3:81976435-81976454 | MS.gene054799:intron | 55.0% | |
| GGGAAAGGTTCACCTTCCGA+TGG | - | chr3.3:81976534-81976553 | MS.gene054799:CDS | 55.0% | |
| GTTCCTGGACATTGCACAGG+TGG | - | chr3.3:81976438-81976457 | MS.gene054799:intron | 55.0% | |
| TTGTGTGGAAGGAGTGGTGG+TGG | + | chr3.3:81976592-81976611 | None:intergenic | 55.0% | |
| !! | CTATGGTGCTGGCCACACAG+TGG | - | chr3.3:81976296-81976315 | MS.gene054799:CDS | 60.0% |
| !! | GTGGAAGGAGTGGTGGTGGT+GGG | + | chr3.3:81976588-81976607 | None:intergenic | 60.0% |
| !! | GTGTGGTGGTGGTTGCAGCA+GGG | + | chr3.3:81976563-81976582 | None:intergenic | 60.0% |
| !! | TGGAAGGAGTGGTGGTGGTG+GGG | + | chr3.3:81976587-81976606 | None:intergenic | 60.0% |
| !! | TGTGGAAGGAGTGGTGGTGG+TGG | + | chr3.3:81976589-81976608 | None:intergenic | 60.0% |
| !! | TGTGGTGGTGGTTGCAGCAG+GGG | + | chr3.3:81976562-81976581 | None:intergenic | 60.0% |
| !! | GAAGGAGTGGTGGTGGTGGG+GGG | + | chr3.3:81976585-81976604 | None:intergenic | 65.0% |
| !! | GGAAGGAGTGGTGGTGGTGG+GGG | + | chr3.3:81976586-81976605 | None:intergenic | 65.0% |
| !! | GGTGTGGTGGTGGTTGCAGC+AGG | + | chr3.3:81976564-81976583 | None:intergenic | 65.0% |
| !! | GGTTGCAGCAGGGGTGCCAT+CGG | + | chr3.3:81976553-81976572 | None:intergenic | 65.0% |
| !! | AGTGGTGGTGGTGGGGGGTG+TGG | + | chr3.3:81976580-81976599 | None:intergenic | 70.0% |
| !! | GCAGCAGGGGTGCCATCGGA+AGG | + | chr3.3:81976549-81976568 | None:intergenic | 70.0% |
| !! | GGTGGTGGGGGGTGTGGTGG+TGG | + | chr3.3:81976574-81976593 | None:intergenic | 75.0% |
| !! | GGTGGTGGTGGGGGGTGTGG+TGG | + | chr3.3:81976577-81976596 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 81975950 | 81976698 | 81975950 | ID=MS.gene054799 |
| chr3.3 | mRNA | 81975950 | 81976698 | 81975950 | ID=MS.gene054799.t1;Parent=MS.gene054799 |
| chr3.3 | exon | 81976530 | 81976698 | 81976530 | ID=MS.gene054799.t1.exon1;Parent=MS.gene054799.t1 |
| chr3.3 | CDS | 81976530 | 81976698 | 81976530 | ID=cds.MS.gene054799.t1;Parent=MS.gene054799.t1 |
| chr3.3 | exon | 81975950 | 81976359 | 81975950 | ID=MS.gene054799.t1.exon2;Parent=MS.gene054799.t1 |
| chr3.3 | CDS | 81975950 | 81976359 | 81975950 | ID=cds.MS.gene054799.t1;Parent=MS.gene054799.t1 |
| Gene Sequence |
| Protein sequence |
