Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60101.t1 | XP_003627814.1 | 97.5 | 118 | 3 | 0 | 1 | 118 | 1 | 118 | 6.60E-56 | 226.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60101.t1 | B8BLZ4 | 71.4 | 119 | 29 | 2 | 1 | 118 | 1 | 115 | 2.4e-38 | 159.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60101.t1 | Q1RU46 | 97.5 | 118 | 3 | 0 | 1 | 118 | 1 | 118 | 4.7e-56 | 226.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene60101.t1 | TR | Others |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049549 | MS.gene60101 | 0.811242 | 7.72E-51 | -1.69E-46 |
| MS.gene052211 | MS.gene60101 | 0.804724 | 1.88E-49 | -1.69E-46 |
| MS.gene05240 | MS.gene60101 | 0.803777 | 2.96E-49 | -1.69E-46 |
| MS.gene054742 | MS.gene60101 | 0.803391 | 3.56E-49 | -1.69E-46 |
| MS.gene059955 | MS.gene60101 | 0.819796 | 9.62E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60101.t1 | MTR_8g038620 | 97.458 | 118 | 3 | 0 | 1 | 118 | 1 | 118 | 1.60e-81 | 236 |
| MS.gene60101.t1 | MTR_7g490310 | 75.630 | 119 | 28 | 1 | 1 | 118 | 1 | 119 | 1.21e-60 | 186 |
| MS.gene60101.t1 | MTR_3g015490 | 81.308 | 107 | 17 | 2 | 8 | 112 | 3 | 108 | 5.54e-56 | 171 |
| MS.gene60101.t1 | MTR_6g007460 | 58.599 | 157 | 17 | 2 | 1 | 117 | 3 | 151 | 4.52e-53 | 166 |
| MS.gene60101.t1 | MTR_3g088630 | 57.377 | 122 | 41 | 2 | 6 | 117 | 19 | 139 | 8.88e-42 | 137 |
| MS.gene60101.t1 | MTR_1g049100 | 61.607 | 112 | 37 | 2 | 7 | 118 | 6 | 111 | 7.37e-39 | 129 |
| MS.gene60101.t1 | MTR_3g093860 | 58.559 | 111 | 36 | 3 | 10 | 118 | 19 | 121 | 2.58e-38 | 126 |
| MS.gene60101.t1 | MTR_5g036480 | 57.143 | 112 | 44 | 1 | 7 | 118 | 14 | 121 | 3.84e-38 | 128 |
| MS.gene60101.t1 | MTR_3g078613 | 55.652 | 115 | 41 | 2 | 4 | 117 | 18 | 123 | 5.13e-36 | 122 |
| MS.gene60101.t1 | MTR_4g106590 | 58.120 | 117 | 38 | 3 | 4 | 118 | 21 | 128 | 1.21e-35 | 121 |
| MS.gene60101.t1 | MTR_3g078613 | 55.172 | 116 | 41 | 3 | 4 | 117 | 18 | 124 | 3.25e-34 | 117 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60101.t1 | AT3G57040 | 67.480 | 123 | 32 | 2 | 1 | 118 | 1 | 120 | 1.43e-52 | 165 |
| MS.gene60101.t1 | AT3G57040 | 67.480 | 123 | 32 | 2 | 1 | 118 | 1 | 120 | 1.43e-52 | 165 |
| MS.gene60101.t1 | AT2G41310 | 65.254 | 118 | 41 | 0 | 1 | 118 | 1 | 118 | 6.97e-51 | 160 |
| MS.gene60101.t1 | AT1G59940 | 61.607 | 112 | 35 | 2 | 7 | 118 | 31 | 134 | 6.08e-41 | 135 |
| MS.gene60101.t1 | AT1G59940 | 61.607 | 112 | 35 | 2 | 7 | 118 | 123 | 226 | 2.34e-39 | 134 |
| MS.gene60101.t1 | AT3G56380 | 53.982 | 113 | 45 | 1 | 6 | 118 | 17 | 122 | 2.56e-39 | 129 |
| MS.gene60101.t1 | AT3G48100 | 55.932 | 118 | 43 | 2 | 2 | 118 | 18 | 127 | 1.08e-38 | 128 |
| MS.gene60101.t1 | AT1G10470 | 58.929 | 112 | 38 | 1 | 7 | 118 | 18 | 121 | 1.80e-38 | 129 |
| MS.gene60101.t1 | AT1G10470 | 58.929 | 112 | 38 | 1 | 7 | 118 | 32 | 135 | 1.99e-38 | 130 |
| MS.gene60101.t1 | AT2G40670 | 53.982 | 113 | 47 | 2 | 6 | 118 | 26 | 133 | 2.30e-38 | 127 |
| MS.gene60101.t1 | AT5G62920 | 56.522 | 115 | 42 | 1 | 4 | 118 | 20 | 126 | 5.06e-38 | 127 |
| MS.gene60101.t1 | AT1G19050 | 56.364 | 110 | 40 | 1 | 8 | 117 | 23 | 124 | 9.25e-38 | 127 |
| MS.gene60101.t1 | AT3G56380 | 53.509 | 114 | 45 | 2 | 6 | 118 | 17 | 123 | 1.65e-37 | 124 |
| MS.gene60101.t1 | AT1G74890 | 55.856 | 111 | 41 | 1 | 8 | 118 | 17 | 119 | 5.86e-37 | 124 |
| MS.gene60101.t1 | AT2G40670 | 52.586 | 116 | 49 | 3 | 4 | 118 | 24 | 134 | 1.56e-36 | 122 |
Find 22 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGTATGTCTTTGAAAGATT+TGG | 0.295699 | 8.4:-59628813 | None:intergenic |
| AGTAAGGCCTTGAAATTTCT+TGG | 0.299900 | 8.4:+59628154 | MS.gene60101:CDS |
| AGAGAATCAGTTCCATGTTT+TGG | 0.307421 | 8.4:+59627892 | MS.gene60101:CDS |
| TCTCACCTATTGATCCTAGA+TGG | 0.331221 | 8.4:-59628863 | None:intergenic |
| TATTGCTTCAGAAATTAATC+AGG | 0.389954 | 8.4:+59628213 | MS.gene60101:CDS |
| TGATTAATTTCTGAAGCAAT+AGG | 0.390281 | 8.4:-59628211 | None:intergenic |
| TACAGTTGATTCCGCTAGTA+AGG | 0.395161 | 8.4:+59628138 | MS.gene60101:CDS |
| AAGCAAACCAAGAAATTTCA+AGG | 0.430996 | 8.4:-59628161 | None:intergenic |
| ATGTTCCATCTAGGATCAAT+AGG | 0.432186 | 8.4:+59628858 | MS.gene60101:CDS |
| TATTGTATGCCAGGGATGAC+TGG | 0.449232 | 8.4:+59628356 | MS.gene60101:CDS |
| ATAACAGATTATTGTATGCC+AGG | 0.463952 | 8.4:+59628347 | MS.gene60101:CDS |
| GAAATTTCTTGGTTTGCTTG+AGG | 0.464582 | 8.4:+59628165 | MS.gene60101:CDS |
| TCTGAGGACATTATCACAAC+AGG | 0.486527 | 8.4:-59628833 | None:intergenic |
| CCTCAGAGAATGTTCCATCT+AGG | 0.494035 | 8.4:+59628849 | MS.gene60101:CDS |
| TTGATAGAATGTTGATTGAG+AGG | 0.516155 | 8.4:+59627933 | MS.gene60101:CDS |
| AGATCATAGCCAGTCATCCC+TGG | 0.546401 | 8.4:-59628365 | None:intergenic |
| TATCTTTCAAGTAATGGGCA+TGG | 0.552535 | 8.4:+59627865 | None:intergenic |
| TAACAGATTATTGTATGCCA+GGG | 0.564090 | 8.4:+59628348 | MS.gene60101:CDS |
| TATCATCAACAGCCAAAACA+TGG | 0.580138 | 8.4:-59627904 | None:intergenic |
| GTTTGCTTGAGGATGAAATG+AGG | 0.583679 | 8.4:+59628176 | MS.gene60101:CDS |
| AAATTTCAAGGCCTTACTAG+CGG | 0.637282 | 8.4:-59628149 | None:intergenic |
| CCTAGATGGAACATTCTCTG+AGG | 0.707424 | 8.4:-59628849 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTATTTTGTTTTTCTATTT+AGG | + | chr8.4:59628549-59628568 | MS.gene60101:intron | 10.0% |
| !! | TACTGAATTGATAAATATTA+TGG | + | chr8.4:59628246-59628265 | MS.gene60101:intron | 15.0% |
| !!! | AGATTAGTTAATTGAAAATT+TGG | - | chr8.4:59628664-59628683 | None:intergenic | 15.0% |
| !!! | GATTAGTTAATTGAAAATTT+GGG | - | chr8.4:59628663-59628682 | None:intergenic | 15.0% |
| !!! | TTGTATAGTCTATTATTTTT+TGG | + | chr8.4:59628057-59628076 | MS.gene60101:intron | 15.0% |
| !! | TAGGAAACAATTTGAAAAAT+AGG | + | chr8.4:59628568-59628587 | MS.gene60101:intron | 20.0% |
| ! | AATATAATTCATTGATCTGC+AGG | + | chr8.4:59628787-59628806 | MS.gene60101:intron | 25.0% |
| ! | TAGACTAATCAAATAACTAG+TGG | - | chr8.4:59628453-59628472 | None:intergenic | 25.0% |
| ! | TATTGCTTCAGAAATTAATC+AGG | + | chr8.4:59628213-59628232 | MS.gene60101:CDS | 25.0% |
| ! | TTGAATGCATCAAAAAAGTT+TGG | + | chr8.4:59628522-59628541 | MS.gene60101:intron | 25.0% |
| ! | TTTGATTCAATTATTCATGC+AGG | + | chr8.4:59628304-59628323 | MS.gene60101:intron | 25.0% |
| !! | CATTAAAAGAAGAAGTTTTG+AGG | - | chr8.4:59627961-59627980 | None:intergenic | 25.0% |
| !! | CTCAAAACTTCTTCTTTTAA+TGG | + | chr8.4:59627959-59627978 | MS.gene60101:CDS | 25.0% |
| !!! | TGATTAATTTCTGAAGCAAT+AGG | - | chr8.4:59628214-59628233 | None:intergenic | 25.0% |
| AAGCAAACCAAGAAATTTCA+AGG | - | chr8.4:59628164-59628183 | None:intergenic | 30.0% | |
| ATAACAGATTATTGTATGCC+AGG | + | chr8.4:59628347-59628366 | MS.gene60101:CDS | 30.0% | |
| ATCACTTCTCACAATTGATT+TGG | + | chr8.4:59628630-59628649 | MS.gene60101:intron | 30.0% | |
| CAAAAGTGAGGAAAAAATTG+TGG | - | chr8.4:59628498-59628517 | None:intergenic | 30.0% | |
| GCATTCAAATTTCAAAAGTG+AGG | - | chr8.4:59628510-59628529 | None:intergenic | 30.0% | |
| GTTGAATATTTATGCTTTCC+AGG | - | chr8.4:59628702-59628721 | None:intergenic | 30.0% | |
| TAACAGATTATTGTATGCCA+GGG | + | chr8.4:59628348-59628367 | MS.gene60101:CDS | 30.0% | |
| TGATCTGTTGAGAAAAATCA+AGG | + | chr8.4:59628382-59628401 | MS.gene60101:CDS | 30.0% | |
| ! | AGGTATGTCTTTGAAAGATT+TGG | - | chr8.4:59628816-59628835 | None:intergenic | 30.0% |
| ! | TTGATAGAATGTTGATTGAG+AGG | + | chr8.4:59627933-59627952 | MS.gene60101:CDS | 30.0% |
| AAATTTCAAGGCCTTACTAG+CGG | - | chr8.4:59628152-59628171 | None:intergenic | 35.0% | |
| AGTAAGGCCTTGAAATTTCT+TGG | + | chr8.4:59628154-59628173 | MS.gene60101:CDS | 35.0% | |
| CTGTTGAGAAAAATCAAGGT+AGG | + | chr8.4:59628386-59628405 | MS.gene60101:intron | 35.0% | |
| TATCATCAACAGCCAAAACA+TGG | - | chr8.4:59627907-59627926 | None:intergenic | 35.0% | |
| TCTCAATCCAAAATTGTTCC+TGG | + | chr8.4:59628681-59628700 | MS.gene60101:intron | 35.0% | |
| ! | AGAGAATCAGTTCCATGTTT+TGG | + | chr8.4:59627892-59627911 | MS.gene60101:CDS | 35.0% |
| ! | ATGCTTTCCAGGAACAATTT+TGG | - | chr8.4:59628691-59628710 | None:intergenic | 35.0% |
| ! | ATGTTCCATCTAGGATCAAT+AGG | + | chr8.4:59628858-59628877 | MS.gene60101:CDS | 35.0% |
| !! | GAAATTTCTTGGTTTGCTTG+AGG | + | chr8.4:59628165-59628184 | MS.gene60101:CDS | 35.0% |
| GTTTGCTTGAGGATGAAATG+AGG | + | chr8.4:59628176-59628195 | MS.gene60101:CDS | 40.0% | |
| TCTGAGGACATTATCACAAC+AGG | - | chr8.4:59628836-59628855 | None:intergenic | 40.0% | |
| ! | TACAGTTGATTCCGCTAGTA+AGG | + | chr8.4:59628138-59628157 | MS.gene60101:CDS | 40.0% |
| CCTAGATGGAACATTCTCTG+AGG | - | chr8.4:59628852-59628871 | None:intergenic | 45.0% | |
| CCTCAGAGAATGTTCCATCT+AGG | + | chr8.4:59628849-59628868 | MS.gene60101:CDS | 45.0% | |
| TATTGTATGCCAGGGATGAC+TGG | + | chr8.4:59628356-59628375 | MS.gene60101:CDS | 45.0% | |
| !! | AAAAATTTATTGTATAAATT+TGG | + | chr8.4:59628274-59628293 | MS.gene60101:intron | 5.0% |
| AGATCATAGCCAGTCATCCC+TGG | - | chr8.4:59628368-59628387 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 59627878 | 59628883 | 59627878 | ID=MS.gene60101 |
| chr8.4 | mRNA | 59627878 | 59628883 | 59627878 | ID=MS.gene60101.t1;Parent=MS.gene60101 |
| chr8.4 | exon | 59627878 | 59627980 | 59627878 | ID=MS.gene60101.t1.exon1;Parent=MS.gene60101.t1 |
| chr8.4 | CDS | 59627878 | 59627980 | 59627878 | ID=cds.MS.gene60101.t1;Parent=MS.gene60101.t1 |
| chr8.4 | exon | 59628134 | 59628234 | 59628134 | ID=MS.gene60101.t1.exon2;Parent=MS.gene60101.t1 |
| chr8.4 | CDS | 59628134 | 59628234 | 59628134 | ID=cds.MS.gene60101.t1;Parent=MS.gene60101.t1 |
| chr8.4 | exon | 59628326 | 59628403 | 59628326 | ID=MS.gene60101.t1.exon3;Parent=MS.gene60101.t1 |
| chr8.4 | CDS | 59628326 | 59628403 | 59628326 | ID=cds.MS.gene60101.t1;Parent=MS.gene60101.t1 |
| chr8.4 | exon | 59628809 | 59628883 | 59628809 | ID=MS.gene60101.t1.exon4;Parent=MS.gene60101.t1 |
| chr8.4 | CDS | 59628809 | 59628883 | 59628809 | ID=cds.MS.gene60101.t1;Parent=MS.gene60101.t1 |
| Gene Sequence |
| Protein sequence |
