Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60460.t1 | PNX54969.1 | 88.2 | 51 | 6 | 0 | 1 | 51 | 18 | 68 | 8.60E-13 | 82 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60460.t1 | A0A2K3JLR2 | 88.2 | 51 | 6 | 0 | 1 | 51 | 18 | 68 | 6.2e-13 | 82.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051091 | MS.gene60460 | -0.803043 | 4.21E-49 | -1.69E-46 |
| MS.gene056525 | MS.gene60460 | -0.804307 | 2.30E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60460.t1 | MTR_3g028450 | 90.196 | 51 | 5 | 0 | 1 | 51 | 18 | 68 | 2.35e-26 | 90.9 |
| MS.gene60460.t1 | MTR_3g028465 | 78.431 | 51 | 11 | 0 | 1 | 51 | 16 | 66 | 1.05e-23 | 84.0 |
| MS.gene60460.t1 | MTR_3g028540 | 82.353 | 51 | 9 | 0 | 1 | 51 | 16 | 66 | 1.45e-23 | 83.6 |
| MS.gene60460.t1 | MTR_3g028480 | 76.471 | 51 | 12 | 0 | 1 | 51 | 16 | 66 | 4.66e-22 | 79.7 |
| MS.gene60460.t1 | MTR_3g028270 | 81.250 | 48 | 9 | 0 | 4 | 51 | 20 | 67 | 8.58e-21 | 76.6 |
| MS.gene60460.t1 | MTR_3g028495 | 88.636 | 44 | 5 | 0 | 8 | 51 | 23 | 66 | 9.03e-21 | 76.6 |
| MS.gene60460.t1 | MTR_3g028260 | 79.167 | 48 | 10 | 0 | 4 | 51 | 20 | 67 | 9.08e-20 | 73.9 |
| MS.gene60460.t1 | MTR_3g027772 | 83.333 | 42 | 7 | 0 | 10 | 51 | 24 | 65 | 1.24e-18 | 71.2 |
| MS.gene60460.t1 | MTR_3g028550 | 74.468 | 47 | 12 | 0 | 5 | 51 | 19 | 65 | 1.71e-17 | 68.2 |
| MS.gene60460.t1 | MTR_3g028340 | 79.545 | 44 | 9 | 0 | 8 | 51 | 25 | 68 | 1.95e-17 | 68.2 |
| MS.gene60460.t1 | MTR_3g028560 | 75.000 | 44 | 11 | 0 | 8 | 51 | 142 | 185 | 2.08e-16 | 68.6 |
| MS.gene60460.t1 | MTR_3g028460 | 76.190 | 42 | 10 | 0 | 10 | 51 | 26 | 67 | 7.51e-16 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 19 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTCCTGATGCCGGAGCAGC+TGG | 0.400610 | 3.2:+19830783 | MS.gene60460:CDS |
| GATCCAGCTGCTCCGGCATC+AGG | 0.412361 | 3.2:-19830786 | None:intergenic |
| CCCTCATTGTCTCCGGCACC+AGG | 0.418787 | 3.2:+19830762 | MS.gene60460:CDS |
| GTCTTGAGCCGAGGCGATAG+AGG | 0.462001 | 3.2:-19830733 | None:intergenic |
| GCTGCTCCGGCATCAGGACC+TGG | 0.469740 | 3.2:-19830780 | None:intergenic |
| CATTTCTTAGTGCTTGAAGA+TGG | 0.480630 | 3.2:-19830862 | None:intergenic |
| TGTAACAGATCCAGCTGCTC+CGG | 0.486059 | 3.2:-19830793 | None:intergenic |
| CCGGCACCAGGTCCTGATGC+CGG | 0.497722 | 3.2:+19830774 | MS.gene60460:CDS |
| CCGGCATCAGGACCTGGTGC+CGG | 0.506330 | 3.2:-19830774 | None:intergenic |
| CCGGAGACAATGAGGGGCTG+AGG | 0.541558 | 3.2:-19830755 | None:intergenic |
| ACCTGGTGCCGGAGACAATG+AGG | 0.555717 | 3.2:-19830763 | None:intergenic |
| ACAAATTCAGCGGCTATCAT+CGG | 0.555852 | 3.2:+19830813 | MS.gene60460:CDS |
| GGCGATAGAGGCAGCCAACA+TGG | 0.557671 | 3.2:-19830721 | None:intergenic |
| GTTGGCTGCCTCTATCGCCT+CGG | 0.577294 | 3.2:+19830725 | MS.gene60460:CDS |
| CCTGGTGCCGGAGACAATGA+GGG | 0.578445 | 3.2:-19830762 | None:intergenic |
| CCTCAGCCCCTCATTGTCTC+CGG | 0.589131 | 3.2:+19830755 | MS.gene60460:CDS |
| GGGGCTGAGGTCTTGAGCCG+AGG | 0.618003 | 3.2:-19830742 | None:intergenic |
| CTGGTGCCGGAGACAATGAG+GGG | 0.652003 | 3.2:-19830761 | None:intergenic |
| TGGATCTGTTACAAATTCAG+CGG | 0.670408 | 3.2:+19830803 | MS.gene60460:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TGGATCTGTTACAAATTCAG+CGG | + | chr3.2:19830803-19830822 | MS.gene60460:CDS | 35.0% | |
| ACAAATTCAGCGGCTATCAT+CGG | + | chr3.2:19830813-19830832 | MS.gene60460:CDS | 40.0% | |
| TGTAACAGATCCAGCTGCTC+CGG | - | chr3.2:19830796-19830815 | None:intergenic | 50.0% | |
| CCTCAGCCCCTCATTGTCTC+CGG | + | chr3.2:19830755-19830774 | MS.gene60460:CDS | 60.0% | |
| GTCTTGAGCCGAGGCGATAG+AGG | - | chr3.2:19830736-19830755 | None:intergenic | 60.0% | |
| GTTGGCTGCCTCTATCGCCT+CGG | + | chr3.2:19830725-19830744 | MS.gene60460:CDS | 60.0% | |
| ! | ACCTGGTGCCGGAGACAATG+AGG | - | chr3.2:19830766-19830785 | None:intergenic | 60.0% |
| ! | CCTGGTGCCGGAGACAATGA+GGG | - | chr3.2:19830765-19830784 | None:intergenic | 60.0% |
| ! | CTGGTGCCGGAGACAATGAG+GGG | - | chr3.2:19830764-19830783 | None:intergenic | 60.0% |
| CCCTCATTGTCTCCGGCACC+AGG | + | chr3.2:19830762-19830781 | MS.gene60460:CDS | 65.0% | |
| CCGGAGACAATGAGGGGCTG+AGG | - | chr3.2:19830758-19830777 | None:intergenic | 65.0% | |
| GATCCAGCTGCTCCGGCATC+AGG | - | chr3.2:19830789-19830808 | None:intergenic | 65.0% | |
| CCGGCACCAGGTCCTGATGC+CGG | + | chr3.2:19830774-19830793 | MS.gene60460:CDS | 70.0% | |
| CCGGCATCAGGACCTGGTGC+CGG | - | chr3.2:19830777-19830796 | None:intergenic | 70.0% | |
| GCTGCTCCGGCATCAGGACC+TGG | - | chr3.2:19830783-19830802 | None:intergenic | 70.0% | |
| GGGGCTGAGGTCTTGAGCCG+AGG | - | chr3.2:19830745-19830764 | None:intergenic | 70.0% | |
| GGTCCTGATGCCGGAGCAGC+TGG | + | chr3.2:19830783-19830802 | MS.gene60460:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 19830723 | 19830878 | 19830723 | ID=MS.gene60460 |
| chr3.2 | mRNA | 19830723 | 19830878 | 19830723 | ID=MS.gene60460.t1;Parent=MS.gene60460 |
| chr3.2 | exon | 19830723 | 19830878 | 19830723 | ID=MS.gene60460.t1.exon1;Parent=MS.gene60460.t1 |
| chr3.2 | CDS | 19830723 | 19830878 | 19830723 | ID=cds.MS.gene60460.t1;Parent=MS.gene60460.t1 |
| Gene Sequence |
| Protein sequence |
