AlfalfaGEDB | MS.gene60534

M. sativa cultivar XinJiangDaYe / MS.gene60534

KEGG: -
GO: GO:0003674 GO:0003824 GO:0005488 GO:0005506 GO:0008150 GO:0008152 GO:0016491 GO:0016705 GO:0020037 GO:0043167 GO:0043169 GO:0046872 GO:0046906 GO:0046914 GO:0048037 GO:0055114 GO:0097159 GO:1901363
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60534.t1	RHN81335.1	97.7	128	3	0	38	165	128	255	1.60E-64	255.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60534.t1	D1MI46	39.7	141	82	1	38	175	127	267	8.7e-24	111.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60534.t1	A0A396JZC6	97.7	128	3	0	38	165	128	255	1.1e-64	255.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene06057	MS.gene60534	0.812211	4.75E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60534.t1	MTR_1g091010	96.875	128	4	0	38	165	128	255	8.50e-86	261
MS.gene60534.t1	MTR_1g091023	96.094	128	5	0	38	165	129	256	2.55e-84	257
MS.gene60534.t1	MTR_1g091003	84.375	128	20	0	38	165	129	256	9.07e-75	232
MS.gene60534.t1	MTR_2g073420	57.692	130	53	1	38	165	139	268	1.47e-42	148
MS.gene60534.t1	MTR_1g055325	56.154	130	55	1	38	165	149	278	9.81e-42	146
MS.gene60534.t1	MTR_8g046210	51.049	143	65	2	38	175	136	278	1.03e-38	138
MS.gene60534.t1	MTR_8g046240	50.350	143	66	2	38	175	136	278	9.95e-38	135
MS.gene60534.t1	MTR_1g103680	43.796	137	75	1	38	172	130	266	4.53e-34	125
MS.gene60534.t1	MTR_5g007450	46.875	128	63	1	38	165	137	259	1.47e-33	124
MS.gene60534.t1	MTR_5g007550	46.875	128	63	1	38	165	137	259	1.58e-33	124
MS.gene60534.t1	MTR_1g103670	44.615	130	70	1	38	165	129	258	3.76e-33	123
MS.gene60534.t1	MTR_1g031700	44.615	130	70	1	38	165	129	258	1.67e-32	121
MS.gene60534.t1	MTR_5g007460	40.714	140	78	1	38	172	104	243	8.58e-32	119
MS.gene60534.t1	MTR_1g037370	46.154	130	68	1	38	165	126	255	2.48e-29	112
MS.gene60534.t1	MTR_2g073480	47.692	130	49	2	38	165	61	173	6.87e-29	110
MS.gene60534.t1	MTR_2g073440	47.692	130	49	2	38	165	128	240	1.05e-28	110
MS.gene60534.t1	MTR_7g075610	38.806	134	78	1	38	167	129	262	7.20e-27	106
MS.gene60534.t1	MTR_7g075390	36.429	140	85	1	38	173	129	268	1.77e-24	99.4
MS.gene60534.t1	MTR_7g075580	35.664	143	88	1	29	167	120	262	3.20e-24	98.6
MS.gene60534.t1	MTR_7g075600	35.556	135	82	1	38	167	129	263	1.41e-22	94.0
MS.gene60534.t1	MTR_0608s0010	36.923	130	66	2	38	165	129	244	4.13e-21	89.0
MS.gene60534.t1	MTR_7g075540	36.641	131	79	1	38	164	129	259	4.59e-21	89.7
MS.gene60534.t1	MTR_1g097240	28.659	164	98	2	5	165	105	252	5.79e-21	89.4
MS.gene60534.t1	MTR_5g056720	32.308	130	69	1	38	167	92	202	7.45e-19	83.2
MS.gene60534.t1	MTR_4g078110	35.385	130	67	2	38	165	123	237	2.05e-18	82.0
MS.gene60534.t1	MTR_4g102310	29.323	133	88	3	37	165	133	263	2.68e-13	67.4
MS.gene60534.t1	MTR_1g116890	31.387	137	83	3	37	165	131	264	2.69e-13	67.4
MS.gene60534.t1	MTR_4g102280	28.571	133	89	2	37	165	138	268	8.13e-12	63.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60534.t1	AT3G61040	33.333	141	88	2	38	173	128	267	4.96e-20	86.3
MS.gene60534.t1	AT3G61040	33.333	141	88	2	38	173	128	267	6.76e-20	86.7
MS.gene60534.t1	AT4G12310	30.667	150	96	2	37	182	138	283	2.24e-17	79.3
MS.gene60534.t1	AT2G45580	31.818	132	86	1	38	165	133	264	2.82e-15	73.2
MS.gene60534.t1	AT2G45580	31.818	132	86	1	38	165	133	264	3.15e-15	73.2
MS.gene60534.t1	AT3G52970	30.328	122	81	2	37	154	131	252	8.54e-15	72.0
MS.gene60534.t1	AT2G45570	31.061	132	88	1	37	165	133	264	1.87e-14	70.9
MS.gene60534.t1	AT2G45550	27.027	148	99	2	37	175	133	280	7.14e-14	69.3
MS.gene60534.t1	AT4G12330	29.286	140	92	2	37	169	140	279	2.15e-13	67.8
MS.gene60534.t1	AT3G52970	27.206	136	81	2	37	154	131	266	4.84e-13	66.6
MS.gene60534.t1	AT4G12320	27.333	150	101	2	37	182	136	281	4.95e-13	66.6
MS.gene60534.t1	AT1G33730	25.180	139	100	2	41	175	1	139	1.50e-12	65.1
MS.gene60534.t1	AT1G33730	25.180	139	100	2	41	175	1	139	1.50e-12	65.1
MS.gene60534.t1	AT4G12300	28.873	142	93	2	38	175	137	274	2.26e-12	64.7
MS.gene60534.t1	AT5G44620	24.096	166	106	2	8	169	127	276	9.25e-11	60.1

Find 19 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACCTTCATACTCCTATAAAT+TGG	0.180918	1.2:+68579989	MS.gene60534:CDS
TCTTGTTCACCCCAATTTAT+AGG	0.237505	1.2:-68580000	None:intergenic
CAAAGAAGTCTTGATATCTT+TGG	0.274361	1.2:+68580051	MS.gene60534:CDS
TTCTCTACCAAGACTCTTGA+TGG	0.280959	1.2:+68580641	MS.gene60534:CDS
TTCAAAAGTGTTCTATGCTT+TGG	0.301447	1.2:+68580952	MS.gene60534:CDS
CCTTCATACTCCTATAAATT+GGG	0.315522	1.2:+68579990	MS.gene60534:CDS
TGTTGGAAGAGCTGCTTTCA+AGG	0.399048	1.2:+68580745	MS.gene60534:CDS
ATGCATAAAAGCAGCTTAAC+CGG	0.422718	1.2:+68580710	MS.gene60534:CDS
TGTGTCTTCTAATGCCTTGT+GGG	0.426560	1.2:-68580916	None:intergenic
AGATATCAAGACTTCTTTGA+AGG	0.447469	1.2:-68580047	None:intergenic
TCCAACATCAACAGCTTCAC+CGG	0.481843	1.2:-68580729	None:intergenic
CCCAATTTATAGGAGTATGA+AGG	0.505744	1.2:-68579990	None:intergenic
TTGACTTCCATCAAGAGTCT+TGG	0.512046	1.2:-68580648	None:intergenic
GGGAAATGATCAGCAATGTT+TGG	0.514705	1.2:-68580875	None:intergenic
TCAACTCTATTAAGTGCTGT+TGG	0.527182	1.2:+68580848	MS.gene60534:CDS
GTGTGTCTTCTAATGCCTTG+TGG	0.537078	1.2:-68580917	None:intergenic
ACCGGTGAAGCTGTTGATGT+TGG	0.556936	1.2:+68580728	MS.gene60534:CDS
GGGGTGAACAAGAACATAAG+TGG	0.586946	1.2:+68580010	MS.gene60534:CDS
CTTCATACTCCTATAAATTG+GGG	0.608665	1.2:+68579991	MS.gene60534:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAGAATAGATAATTAAAAAT+AGG	-	chr1.2:68580101-68580120	None:intergenic	10.0%
!!	ATGAACACTACTAAAAAAAT+TGG	+	chr1.2:68580995-68581014	MS.gene60534:CDS	20.0%
!!	ATTTGTAAAAAGAATCTCTT+TGG	-	chr1.2:68580492-68580511	None:intergenic	20.0%
!!	TGAACACTACTAAAAAAATT+GGG	+	chr1.2:68580996-68581015	MS.gene60534:CDS	20.0%
!	AAAAAAATTGGGAATAGAGA+CGG	+	chr1.2:68581007-68581026	MS.gene60534:CDS	25.0%
!	AGCATTAACATATATAAAGG+TGG	-	chr1.2:68580163-68580182	None:intergenic	25.0%
!	ATTAACATATATAAAGGTGG+AGG	-	chr1.2:68580160-68580179	None:intergenic	25.0%
!	TGCAGCATTAACATATATAA+AGG	-	chr1.2:68580166-68580185	None:intergenic	25.0%
!!	ATTTCAGATATGGATTATGT+TGG	+	chr1.2:68580221-68580240	MS.gene60534:intron	25.0%
!!	TAATCCATATCTGAAATCAT+CGG	-	chr1.2:68580218-68580237	None:intergenic	25.0%
	AAAAAATTGGGAATAGAGAC+GGG	+	chr1.2:68581008-68581027	MS.gene60534:CDS	30.0%
	AATCATCTCATCATCACATA+TGG	+	chr1.2:68580466-68580485	MS.gene60534:intron	30.0%
	ACCTTCATACTCCTATAAAT+TGG	+	chr1.2:68579989-68580008	MS.gene60534:CDS	30.0%
	AGATATCAAGACTTCTTTGA+AGG	-	chr1.2:68580050-68580069	None:intergenic	30.0%
	CAAAGAAGTCTTGATATCTT+TGG	+	chr1.2:68580051-68580070	MS.gene60534:CDS	30.0%
	CATAACTTAATGCGCATAAT+AGG	-	chr1.2:68580431-68580450	None:intergenic	30.0%
	CCTTCATACTCCTATAAATT+GGG	+	chr1.2:68579990-68580009	MS.gene60534:CDS	30.0%
	CTTCATACTCCTATAAATTG+GGG	+	chr1.2:68579991-68580010	MS.gene60534:CDS	30.0%
!	ATGTGATGATGAGATGATTA+TGG	-	chr1.2:68580465-68580484	None:intergenic	30.0%
!	GACATGATAGTGAAAAAAGA+AGG	-	chr1.2:68580344-68580363	None:intergenic	30.0%
!!	TTCAAAAGTGTTCTATGCTT+TGG	+	chr1.2:68580952-68580971	MS.gene60534:CDS	30.0%
	AGTTGTAGTGTTATCAGGAA+CGG	-	chr1.2:68580537-68580556	None:intergenic	35.0%
	ATAGTGAAAAAAGAAGGTCC+TGG	-	chr1.2:68580338-68580357	None:intergenic	35.0%
	ATGCATAAAAGCAGCTTAAC+CGG	+	chr1.2:68580710-68580729	MS.gene60534:CDS	35.0%
	GGTCAAAAATCTTCAACACT+GGG	-	chr1.2:68580898-68580917	None:intergenic	35.0%
	TCTTGTTCACCCCAATTTAT+AGG	-	chr1.2:68580003-68580022	None:intergenic	35.0%
	TGACAATGCATGTCAACAAA+AGG	-	chr1.2:68580259-68580278	None:intergenic	35.0%
	TGCATTGTCATGTTCTTCAT+TGG	+	chr1.2:68580269-68580288	MS.gene60534:intron	35.0%
	TTGTGAGTTGTAGTGTTATC+AGG	-	chr1.2:68580542-68580561	None:intergenic	35.0%
!	AACTTTGCAAGTGTTTGTTG+TGG	-	chr1.2:68580392-68580411	None:intergenic	35.0%
!	CCCAATTTATAGGAGTATGA+AGG	-	chr1.2:68579993-68580012	None:intergenic	35.0%
!	TCAACTCTATTAAGTGCTGT+TGG	+	chr1.2:68580848-68580867	MS.gene60534:CDS	35.0%
!	TTGAAGATTTTTGACCCACA+AGG	+	chr1.2:68580902-68580921	MS.gene60534:CDS	35.0%
!!	AACACTTGCAAAGTTTGCTA+AGG	+	chr1.2:68580397-68580416	MS.gene60534:intron	35.0%
	GGGAAATGATCAGCAATGTT+TGG	-	chr1.2:68580878-68580897	None:intergenic	40.0%
	GGGTCAAAAATCTTCAACAC+TGG	-	chr1.2:68580899-68580918	None:intergenic	40.0%
	GTTGCCAAAGAGGTGAAAAT+GGG	-	chr1.2:68580592-68580611	None:intergenic	40.0%
	TCCTCCGATGATTTCAGATA+TGG	+	chr1.2:68580211-68580230	MS.gene60534:intron	40.0%
	TGTGTCTTCTAATGCCTTGT+GGG	-	chr1.2:68580919-68580938	None:intergenic	40.0%
	TGTTGCCAAAGAGGTGAAAA+TGG	-	chr1.2:68580593-68580612	None:intergenic	40.0%
	TTCCTAAGATGTTGCCAAAG+AGG	-	chr1.2:68580602-68580621	None:intergenic	40.0%
	TTCTCTACCAAGACTCTTGA+TGG	+	chr1.2:68580641-68580660	MS.gene60534:CDS	40.0%
	TTGACTTCCATCAAGAGTCT+TGG	-	chr1.2:68580651-68580670	None:intergenic	40.0%
!	TCCATATCTGAAATCATCGG+AGG	-	chr1.2:68580215-68580234	None:intergenic	40.0%
!!	GCAAAGTTTGCTAAGGTCTA+TGG	+	chr1.2:68580404-68580423	MS.gene60534:intron	40.0%
	CACCTCTTTGGCAACATCTT+AGG	+	chr1.2:68580597-68580616	MS.gene60534:intron	45.0%
	GGGGTGAACAAGAACATAAG+TGG	+	chr1.2:68580010-68580029	MS.gene60534:CDS	45.0%
	GTGAAAAAAGAAGGTCCTGG+TGG	-	chr1.2:68580335-68580354	None:intergenic	45.0%
	GTGTGTCTTCTAATGCCTTG+TGG	-	chr1.2:68580920-68580939	None:intergenic	45.0%
	TCCAACATCAACAGCTTCAC+CGG	-	chr1.2:68580732-68580751	None:intergenic	45.0%
!	TCCTCCCATTTTCACCTCTT+TGG	+	chr1.2:68580585-68580604	MS.gene60534:intron	45.0%
!	TGTTGGAAGAGCTGCTTTCA+AGG	+	chr1.2:68580745-68580764	MS.gene60534:CDS	45.0%
	ACCGGTGAAGCTGTTGATGT+TGG	+	chr1.2:68580728-68580747	MS.gene60534:CDS	50.0%
	GCCAAAGAGGTGAAAATGGG+AGG	-	chr1.2:68580589-68580608	None:intergenic	50.0%
	TCGAACTACAAGCTTCCACC+AGG	+	chr1.2:68580317-68580336	MS.gene60534:intron	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	68579976	68581051	68579976	ID=MS.gene60534
chr1.2	mRNA	68579976	68581051	68579976	ID=MS.gene60534.t1;Parent=MS.gene60534
chr1.2	exon	68579976	68580091	68579976	ID=MS.gene60534.t1.exon1;Parent=MS.gene60534.t1
chr1.2	CDS	68579976	68580091	68579976	ID=cds.MS.gene60534.t1;Parent=MS.gene60534.t1
chr1.2	exon	68580619	68581051	68580619	ID=MS.gene60534.t1.exon2;Parent=MS.gene60534.t1
chr1.2	CDS	68580619	68581051	68580619	ID=cds.MS.gene60534.t1;Parent=MS.gene60534.t1

Gene Sequence

>MS.gene60534

ATGAACGTAATTAACCTTCATACTCCTATAAATTGGGGTGAACAAGAACATAAGTGGCAAGAACAAGAAGTCCTTCAAAGAAGTCTTGATATCTTTGGTTCACTTTTAGTACTTAAGTAAGTCCTATTTTTAATTATCTATTCTTTCTTAATTATTATTTCAAGCTTTATAATCAATATTTCCTCCACCTTTATATATGTTAATGCTGCATGTATGAAATTAATAAAGAACTTGTTCCTCCGATGATTTCAGATATGGATTATGTTGGTAGTGTTATGCTCCTTTTGTTGACATGCATTGTCATGTTCTTCATTGGTTCACTACATGCAAGAAGCAAAAAATCGAACTACAAGCTTCCACCAGGACCTTCTTTTTTCACTATCATGTCACATGTTGTTGAATTGTACAACAAGCCACAACAAACACTTGCAAAGTTTGCTAAGGTCTATGGTCCTATTATGCGCATTAAGTTATGCACAGAAACTACCATAATCATCTCATCATCACATATGGCCAAAGAGATTCTTTTTACAAATGATTCATTGTTCACTGATAGATCCGTTCCTGATAACACTACAACTCACAATCACAACAATTTTAGCTTAGTTTTCCTCCCATTTTCACCTCTTTGGCAACATCTTAGGAAAATATGTCATAATAATTTATTCTCTACCAAGACTCTTGATGGAAGTCAAGAACTTAGACGTATGAAATTAAAAGATCTTCTCAATGATATGCATAAAAGCAGCTTAACCGGTGAAGCTGTTGATGTTGGAAGAGCTGCTTTCAAGGCTTGCATTAATTTTTTGTCATACACTTTTGTGTCTCAAGATTTTGTTGAGAATTTAGATGATGAATATAAAAATATAGTGTCAACTCTATTAAGTGCTGTTGGAACACCAAACATTGCTGATCATTTCCCAGTGTTGAAGATTTTTGACCCACAAGGCATTAGAAGACACACTACTCTTTATGTTTCAAAAGTGTTCTATGCTTTGGATATCATAATTGACAAACGAATGAACACTACTAAAAAAATTGGGAATAGAGACGGGCAAAATCCGTCTCTAAAATGA

Protein sequence

>MS.gene60534.t1

MNVINLHTPINWGEQEHKWQEQEVLQRSLDIFGSLLVLKKICHNNLFSTKTLDGSQELRRMKLKDLLNDMHKSSLTGEAVDVGRAAFKACINFLSYTFVSQDFVENLDDEYKNIVSTLLSAVGTPNIADHFPVLKIFDPQGIRRHTTLYVSKVFYALDIIIDKRMNTTKKIGNRDGQNPSLK