AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene60555

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60555.t1 MTR_1g492730 97.049 305 9 0 1 305 1 305 0.0 618
MS.gene60555.t1 MTR_1g492730 95.714 210 9 0 1 210 1 210 2.32e-143 402
MS.gene60555.t1 MTR_1g052405 46.411 209 90 4 102 304 135 327 3.56e-58 190
MS.gene60555.t1 MTR_3g085620 51.825 137 64 1 167 303 40 174 6.57e-49 161
MS.gene60555.t1 MTR_1g052380 47.945 146 73 2 159 303 50 193 3.94e-43 147
MS.gene60555.t1 MTR_4g014750 35.294 153 83 5 165 302 77 228 7.59e-16 75.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60555.t1 AT3G57810 58.571 140 56 1 163 302 166 303 1.12e-55 183
MS.gene60555.t1 AT3G57810 58.571 140 56 1 163 302 166 303 1.12e-55 183
MS.gene60555.t1 AT3G57810 58.571 140 56 1 163 302 166 303 1.12e-55 183
MS.gene60555.t1 AT2G38025 32.075 159 86 4 165 302 53 210 4.32e-15 73.2
MS.gene60555.t1 AT2G38025 32.075 159 86 4 165 302 76 233 7.81e-15 72.8
MS.gene60555.t1 AT3G57810 39.362 94 52 2 123 211 121 214 1.72e-14 71.6

Find 96 sgRNAs with CRISPR-Local

Find 144 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGAACTTGCCGACGAATTA+AGG 0.213414 1.2:+68869392 MS.gene60555:CDS
CTCCACCGCCGGTTGAGATT+TGG 0.298384 1.2:-68868319 None:intergenic
AACATAAGTCTGTTGAATTC+TGG 0.316175 1.2:-68869708 None:intergenic
TGGAATGGTGGCGGCGGGCG+GGG 0.325826 1.2:-68868280 None:intergenic
TGAGATTTGGAGGTGGTTGC+AGG 0.327466 1.2:-68868306 None:intergenic
GGTGGAATGGTGGCGGCGGG+CGG 0.330689 1.2:-68868282 None:intergenic
AATTCAACAGACTTATGTTT+GGG 0.341402 1.2:+68869712 MS.gene60555:CDS
AGGAGAGCCTGAGCTGTTAA+TGG 0.343536 1.2:+68869736 MS.gene60555:CDS
TCTCTCATGAAGACATATAT+TGG 0.349253 1.2:-68870184 None:intergenic
GTGGAATGGTGGCGGCGGGC+GGG 0.352247 1.2:-68868281 None:intergenic
GCGCCACTCGCTCTCGTCCT+TGG 0.358149 1.2:+68868189 MS.gene60555:CDS
AGATGCAAGCTCCATGGATT+TGG 0.364917 1.2:+68870204 MS.gene60555:CDS
CTGCGCTTGCCTTGCGCCGC+CGG 0.373717 1.2:+68868517 MS.gene60555:CDS
GAAAGGCGTGGTGGTGGTGG+TGG 0.384833 1.2:-68868223 None:intergenic
ACATGGTCACTACGAACTAT+TGG 0.387672 1.2:+68870297 MS.gene60555:CDS
TGGAGTCGGCGGCGCCGCCT+CGG 0.392242 1.2:-68868558 None:intergenic
GAATTCAACAGACTTATGTT+TGG 0.396680 1.2:+68869711 MS.gene60555:CDS
TCGATGAAATAGCACATTGA+TGG 0.414542 1.2:-68870275 None:intergenic
GGCGGTGGAATGGTGGCGGC+GGG 0.418826 1.2:-68868285 None:intergenic
ATCTTTCACTTCACGTCCCT+CGG 0.423234 1.2:-68868603 None:intergenic
GAAGCATCACACCGCTGCTT+CGG 0.424353 1.2:-68868388 None:intergenic
TCCCTCTGTCGACTTTCATT+AGG 0.424371 1.2:-68869373 None:intergenic
CTCTCGTCCGGGAAAACAGC+TGG 0.428780 1.2:-68868578 None:intergenic
TTGGAGGTGGTTGCAGGCGG+TGG 0.429035 1.2:-68868300 None:intergenic
ATTACTCCTTGCGGCGGCGA+TGG 0.436846 1.2:+68868362 MS.gene60555:CDS
GGAACTTGCCGACGAATTAA+GGG 0.438594 1.2:+68869393 MS.gene60555:CDS
CGTCCCTCGGAACTCTCGTC+CGG 0.439061 1.2:-68868590 None:intergenic
GAGTTCATCGTCACGTTCAT+CGG 0.445917 1.2:-68868633 None:intergenic
AAGGATCGTGGAACGTTGCT+TGG 0.447140 1.2:+68868435 MS.gene60555:CDS
TTCATCGTTCTGACTCAGCT+TGG 0.448002 1.2:+68868480 MS.gene60555:CDS
TGCTATATTTACCAAATCCA+TGG 0.450384 1.2:-68870215 None:intergenic
TGTGTCTATAGGTTTATAGA+AGG 0.457572 1.2:+68869665 MS.gene60555:intron
GCGGTGGAGCTGCTTCGATA+TGG 0.467969 1.2:+68868333 MS.gene60555:CDS
GACGAAAGGCGTGGTGGTGG+TGG 0.470943 1.2:-68868226 None:intergenic
AGGCGGTGGAATGGTGGCGG+CGG 0.476240 1.2:-68868286 None:intergenic
CACCGCCGGTTGAGATTTGG+AGG 0.479044 1.2:-68868316 None:intergenic
GTATCAACGTCAACAATAAC+CGG 0.479760 1.2:-68868536 None:intergenic
CTTGGGATGCTCGTCCTGCT+AGG 0.480235 1.2:+68868453 MS.gene60555:CDS
TACTTTAACCCTTAATTCGT+CGG 0.484358 1.2:-68869401 None:intergenic
AAAGGCGTGGTGGTGGTGGT+GGG 0.493789 1.2:-68868222 None:intergenic
CTCCAAATCTCAACCGGCGG+TGG 0.496363 1.2:+68868317 MS.gene60555:CDS
GGTGGTTGCAGGCGGTGGAA+TGG 0.499927 1.2:-68868295 None:intergenic
GCCACTCACTCATCCATGCT+TGG 0.503058 1.2:+68868158 None:intergenic
ATCCTTCACCTTTGAGCTCG+TGG 0.505031 1.2:-68868418 None:intergenic
TTGGTAAATATAGCAAAATA+CGG 0.508333 1.2:+68870223 MS.gene60555:CDS
CGCCGGTTGAGATTTGGAGG+TGG 0.513387 1.2:-68868313 None:intergenic
AACCACCTCCAAATCTCAAC+CGG 0.518771 1.2:+68868311 MS.gene60555:CDS
AGGATCGTGGAACGTTGCTT+GGG 0.525778 1.2:+68868436 MS.gene60555:CDS
TGCAGGCGGTGGAATGGTGG+CGG 0.528482 1.2:-68868289 None:intergenic
CGGCGGGCGGGGAAAGTGAG+AGG 0.534229 1.2:-68868269 None:intergenic
TGAGAAGCCATTAACAGCTC+AGG 0.536531 1.2:-68869743 None:intergenic
GTCCCTCGGAACTCTCGTCC+GGG 0.539858 1.2:-68868589 None:intergenic
CTGTTCAGAGCAATAGCTCA+TGG 0.539965 1.2:+68869322 MS.gene60555:CDS
GTGTCTATAGGTTTATAGAA+GGG 0.543445 1.2:+68869666 MS.gene60555:intron
TGTCTATAGGTTTATAGAAG+GGG 0.544260 1.2:+68869667 MS.gene60555:intron
GAACGATGAAGCCACCTAGC+AGG 0.545883 1.2:-68868467 None:intergenic
GGTTGCAGGCGGTGGAATGG+TGG 0.547561 1.2:-68868292 None:intergenic
TAGGGGTGCTTGCTGATGGT+CGG 0.550798 1.2:+68869296 MS.gene60555:intron
CAACAATAACCGGCGGCGCA+AGG 0.554275 1.2:-68868526 None:intergenic
CGGAATCCATCGCCGCCGCA+AGG 0.559044 1.2:-68868368 None:intergenic
GGCGCAGAGTACACCAAGCA+TGG 0.563817 1.2:-68868171 None:intergenic
AGCTCAAAGGTGAAGGATCG+TGG 0.569548 1.2:+68868423 MS.gene60555:CDS
AACGCTGATTACAGAGTCAC+GGG 0.570421 1.2:+68868656 MS.gene60555:CDS
CATGAGAGATGCAAGCTCCA+TGG 0.570844 1.2:+68870198 MS.gene60555:CDS
TCCGGGAAAACAGCTGGAGT+CGG 0.574081 1.2:-68868572 None:intergenic
CAACAGACTTATGTTTGGGG+AGG 0.576052 1.2:+68869716 MS.gene60555:CDS
GACCACGAGCTCAAAGGTGA+AGG 0.577751 1.2:+68868416 MS.gene60555:CDS
GATCCAAGGACGAGAGCGAG+TGG 0.579496 1.2:-68868192 None:intergenic
GGTATAGGGGTGCTTGCTGA+TGG 0.584649 1.2:+68869292 MS.gene60555:intron
TATCGAAGCAGCTCCACCGC+CGG 0.586713 1.2:-68868330 None:intergenic
CATCATGACCACGAGCTCAA+AGG 0.587256 1.2:+68868410 MS.gene60555:CDS
TTCCCGGACGAGAGTTCCGA+GGG 0.588950 1.2:+68868587 MS.gene60555:CDS
AGAGACGAAAGGCGTGGTGG+TGG 0.589008 1.2:-68868229 None:intergenic
GAAAGTGAGAGGAGCGACAG+TGG 0.606183 1.2:-68868258 None:intergenic
CAACGCTGATTACAGAGTCA+CGG 0.607136 1.2:+68868655 MS.gene60555:CDS
CACGCGATTACTCCTTGCGG+CGG 0.611520 1.2:+68868356 MS.gene60555:CDS
TTTCCCGGACGAGAGTTCCG+AGG 0.612131 1.2:+68868586 MS.gene60555:CDS
GCCTAATGAAAGTCGACAGA+GGG 0.612158 1.2:+68869372 MS.gene60555:CDS
GGCGATGGATTCCGAAGCAG+CGG 0.621773 1.2:+68868377 MS.gene60555:CDS
TATTGTTGACGTTGATACCG+AGG 0.628764 1.2:+68868541 MS.gene60555:CDS
GGGAAAACAGCTGGAGTCGG+CGG 0.628808 1.2:-68868569 None:intergenic
TGAAGAATATATGAATGATG+AGG 0.630448 1.2:+68870246 MS.gene60555:CDS
GATTTGGAGGTGGTTGCAGG+CGG 0.637060 1.2:-68868303 None:intergenic
TGGTGGGAAGAGAAGATCCA+AGG 0.650314 1.2:-68868206 None:intergenic
CGCCTAATGAAAGTCGACAG+AGG 0.650904 1.2:+68869371 MS.gene60555:CDS
CACCTCCAAATCTCAACCGG+CGG 0.653859 1.2:+68868314 MS.gene60555:CDS
GGGATGCTCGTCCTGCTAGG+TGG 0.655002 1.2:+68868456 MS.gene60555:CDS
ACAGGCGCAAGGAAACAGAA+TGG 0.661069 1.2:+68869554 MS.gene60555:CDS
ATTCAACAGACTTATGTTTG+GGG 0.663997 1.2:+68869713 MS.gene60555:CDS
AATGTGCTATTTCATCGACA+TGG 0.668256 1.2:+68870280 MS.gene60555:CDS
GAGAGAGACGAAAGGCGTGG+TGG 0.678901 1.2:-68868232 None:intergenic
CGTGAGAGAGACGAAAGGCG+TGG 0.686067 1.2:-68868235 None:intergenic
AGTGGCGTGAGAGAGACGAA+AGG 0.697149 1.2:-68868240 None:intergenic
TGGCACGCGATTACTCCTTG+CGG 0.725561 1.2:+68868353 MS.gene60555:CDS
TGTTGACGTTGATACCGAGG+CGG 0.731095 1.2:+68868544 MS.gene60555:CDS
TCAACGTCAACAATAACCGG+CGG 0.746562 1.2:-68868533 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTACTTTTATTTACA+TGG + chr1.2:68869621-68869640 MS.gene60555:intron 10.0%
!! AAATAAAACATCACAAAAAT+TGG - chr1.2:68869513-68869532 None:intergenic 15.0%
!! AATAAAACATCACAAAAATT+GGG - chr1.2:68869512-68869531 None:intergenic 15.0%
!!! ATTTCATTTTTTGTCTTTAA+TGG + chr1.2:68868838-68868857 MS.gene60555:intron 15.0%
!!! ATTTTTGTGATGTTTTATTT+AGG + chr1.2:68869513-68869532 MS.gene60555:intron 15.0%
!!! TTTTTTTTCATATAAGTACA+GGG + chr1.2:68868865-68868884 MS.gene60555:intron 15.0%
!!! TTTTTTTTTCATATAAGTAC+AGG + chr1.2:68868864-68868883 MS.gene60555:intron 15.0%
!! TTGGTAAATATAGCAAAATA+CGG + chr1.2:68870223-68870242 MS.gene60555:CDS 20.0%
!!! CTTTTATTTACATGGTAAAT+TGG + chr1.2:68869629-68869648 MS.gene60555:intron 20.0%
! AACAAGAACTATTGTATCAT+TGG - chr1.2:68869114-68869133 None:intergenic 25.0%
! AATCGATGGATATTAATCTA+CGG + chr1.2:68869984-68870003 MS.gene60555:intron 25.0%
! AATTCAACAGACTTATGTTT+GGG + chr1.2:68869712-68869731 MS.gene60555:CDS 25.0%
! CTTATAGAGAAACAAATTTC+CGG + chr1.2:68870041-68870060 MS.gene60555:intron 25.0%
! GGAAACTACATACAATTATT+TGG + chr1.2:68869595-68869614 MS.gene60555:intron 25.0%
! TAAGTTATGAAGACAATGTT+AGG - chr1.2:68870156-68870175 None:intergenic 25.0%
! TATAAGTTTGTATCACTGAA+TGG + chr1.2:68869271-68869290 MS.gene60555:intron 25.0%
! TGAAGAATATATGAATGATG+AGG + chr1.2:68870246-68870265 MS.gene60555:CDS 25.0%
!!! TCATTGTTCAGTTATTTTGA+TGG + chr1.2:68869200-68869219 MS.gene60555:intron 25.0%
AACATAAGTCTGTTGAATTC+TGG - chr1.2:68869711-68869730 None:intergenic 30.0%
AACATGAGTTAGAGATTATG+TGG + chr1.2:68869241-68869260 MS.gene60555:intron 30.0%
AAGAATGTTGACAAAGAAGA+AGG - chr1.2:68869465-68869484 None:intergenic 30.0%
ACAAGTTAAACTGTTGTTGT+AGG - chr1.2:68869923-68869942 None:intergenic 30.0%
ACATGAGTTAGAGATTATGT+GGG + chr1.2:68869242-68869261 MS.gene60555:intron 30.0%
ACTTGTTCAATATGATCTGT+CGG + chr1.2:68869151-68869170 MS.gene60555:intron 30.0%
AGTAGCATAATGATAATCGA+TGG + chr1.2:68869970-68869989 MS.gene60555:intron 30.0%
ATATAAGTACAGGGATTGTT+TGG + chr1.2:68868874-68868893 MS.gene60555:intron 30.0%
ATCGATGGATATTAATCTAC+GGG + chr1.2:68869985-68870004 MS.gene60555:intron 30.0%
ATTCAACAGACTTATGTTTG+GGG + chr1.2:68869713-68869732 MS.gene60555:CDS 30.0%
CATTGTCTTCATAACTTACA+AGG + chr1.2:68870158-68870177 MS.gene60555:intron 30.0%
GAATTCAACAGACTTATGTT+TGG + chr1.2:68869711-68869730 MS.gene60555:CDS 30.0%
GTGTCTATAGGTTTATAGAA+GGG + chr1.2:68869666-68869685 MS.gene60555:intron 30.0%
TACTTTAACCCTTAATTCGT+CGG - chr1.2:68869404-68869423 None:intergenic 30.0%
TCTCTCATGAAGACATATAT+TGG - chr1.2:68870187-68870206 None:intergenic 30.0%
TGCTATATTTACCAAATCCA+TGG - chr1.2:68870218-68870237 None:intergenic 30.0%
TGGGTAACAAAATTCATTGA+AGG - chr1.2:68869493-68869512 None:intergenic 30.0%
TGTCTATAGGTTTATAGAAG+GGG + chr1.2:68869667-68869686 MS.gene60555:intron 30.0%
TGTGTCTATAGGTTTATAGA+AGG + chr1.2:68869665-68869684 MS.gene60555:intron 30.0%
TTACAACTACGAAACAAGAA+CGG + chr1.2:68869012-68869031 MS.gene60555:intron 30.0%
TTGTATCACTGAATGGTATA+GGG + chr1.2:68869278-68869297 MS.gene60555:intron 30.0%
TTTGTATCACTGAATGGTAT+AGG + chr1.2:68869277-68869296 MS.gene60555:intron 30.0%
! TCATTTTGTGGTAAATCGTT+GGG + chr1.2:68870065-68870084 MS.gene60555:intron 30.0%
! TTCATTTTGTGGTAAATCGT+TGG + chr1.2:68870064-68870083 MS.gene60555:intron 30.0%
!! AACAACAGTTTAACTTGTTG+TGG + chr1.2:68869925-68869944 MS.gene60555:intron 30.0%
!! GTTATATTTGCTGTGTCTAT+AGG + chr1.2:68869654-68869673 MS.gene60555:intron 30.0%
!! TCTTGCTAGAACTTTTGAAA+CGG + chr1.2:68869430-68869449 MS.gene60555:intron 30.0%
ATTGAACAAGTGTCTTGAGT+TGG - chr1.2:68869142-68869161 None:intergenic 35.0%
CAATGTTAGGTACAACGAAA+TGG - chr1.2:68870143-68870162 None:intergenic 35.0%
CGATTTACCACAAAATGAAC+CGG - chr1.2:68870063-68870082 None:intergenic 35.0%
GTATCAACGTCAACAATAAC+CGG - chr1.2:68868539-68868558 None:intergenic 35.0%
TCGATGAAATAGCACATTGA+TGG - chr1.2:68870278-68870297 None:intergenic 35.0%
TGTATCACTGAATGGTATAG+GGG + chr1.2:68869279-68869298 MS.gene60555:intron 35.0%
! CAAATTTCCGGTTCATTTTG+TGG + chr1.2:68870053-68870072 MS.gene60555:intron 35.0%
! TTGCTGAAGAACTTTTGAAC+AGG + chr1.2:68869536-68869555 MS.gene60555:CDS 35.0%
!! AATGTGCTATTTCATCGACA+TGG + chr1.2:68870280-68870299 MS.gene60555:CDS 35.0%
!! TGGTGAGCTTTGTTATTTGA+AGG + chr1.2:68869574-68869593 MS.gene60555:intron 35.0%
ACATGGTCACTACGAACTAT+TGG + chr1.2:68870297-68870316 MS.gene60555:CDS 40.0%
GTGACAAACCATCAAACACT+TGG - chr1.2:68868983-68869002 None:intergenic 40.0%
GTTTGTGACCAAGTGTTTGA+TGG + chr1.2:68868972-68868991 MS.gene60555:intron 40.0%
TATTGTTGACGTTGATACCG+AGG + chr1.2:68868541-68868560 MS.gene60555:CDS 40.0%
! AGCTTGCTTGAAAAATGGAG+AGG + chr1.2:68869345-68869364 MS.gene60555:CDS 40.0%
! CATGGAGCTTGCTTGAAAAA+TGG + chr1.2:68869340-68869359 MS.gene60555:CDS 40.0%
!!! CTAGAACTTTTGAAACGGAG+AGG + chr1.2:68869435-68869454 MS.gene60555:intron 40.0%
AACCACCTCCAAATCTCAAC+CGG + chr1.2:68868311-68868330 MS.gene60555:CDS 45.0%
AGATGCAAGCTCCATGGATT+TGG + chr1.2:68870204-68870223 MS.gene60555:CDS 45.0%
ATCTTTCACTTCACGTCCCT+CGG - chr1.2:68868606-68868625 None:intergenic 45.0%
CAACAGACTTATGTTTGGGG+AGG + chr1.2:68869716-68869735 MS.gene60555:CDS 45.0%
CTGTTCAGAGCAATAGCTCA+TGG + chr1.2:68869322-68869341 MS.gene60555:CDS 45.0%
GAGTTCATCGTCACGTTCAT+CGG - chr1.2:68868636-68868655 None:intergenic 45.0%
GCCTAATGAAAGTCGACAGA+GGG + chr1.2:68869372-68869391 MS.gene60555:CDS 45.0%
GGAACTTGCCGACGAATTAA+GGG + chr1.2:68869393-68869412 MS.gene60555:CDS 45.0%
GTGGTAAATCGTTGGGACTT+GGG + chr1.2:68870072-68870091 MS.gene60555:intron 45.0%
TCAACGTCAACAATAACCGG+CGG - chr1.2:68868536-68868555 None:intergenic 45.0%
TGAGAAGCCATTAACAGCTC+AGG - chr1.2:68869746-68869765 None:intergenic 45.0%
TGTGGTAAATCGTTGGGACT+TGG + chr1.2:68870071-68870090 MS.gene60555:intron 45.0%
TTCATCGTTCTGACTCAGCT+TGG + chr1.2:68868480-68868499 MS.gene60555:CDS 45.0%
! AACGCTGATTACAGAGTCAC+GGG + chr1.2:68868656-68868675 MS.gene60555:CDS 45.0%
! AGAACTTTTGAACAGGCGCA+AGG + chr1.2:68869543-68869562 MS.gene60555:CDS 45.0%
! CAACGCTGATTACAGAGTCA+CGG + chr1.2:68868655-68868674 MS.gene60555:CDS 45.0%
! GACTCAGCTTGGCTTCTTTT+CGG + chr1.2:68868491-68868510 MS.gene60555:CDS 45.0%
!! TCCCTCTGTCGACTTTCATT+AGG - chr1.2:68869376-68869395 None:intergenic 45.0%
AAGGATCGTGGAACGTTGCT+TGG + chr1.2:68868435-68868454 MS.gene60555:CDS 50.0%
ACAGGCGCAAGGAAACAGAA+TGG + chr1.2:68869554-68869573 MS.gene60555:CDS 50.0%
AGCTCAAAGGTGAAGGATCG+TGG + chr1.2:68868423-68868442 MS.gene60555:CDS 50.0%
AGGAGAGCCTGAGCTGTTAA+TGG + chr1.2:68869736-68869755 MS.gene60555:CDS 50.0%
AGGATCGTGGAACGTTGCTT+GGG + chr1.2:68868436-68868455 MS.gene60555:CDS 50.0%
ATCCTTCACCTTTGAGCTCG+TGG - chr1.2:68868421-68868440 None:intergenic 50.0%
CATCATGACCACGAGCTCAA+AGG + chr1.2:68868410-68868429 MS.gene60555:CDS 50.0%
CATGAGAGATGCAAGCTCCA+TGG + chr1.2:68870198-68870217 MS.gene60555:CDS 50.0%
CGCCTAATGAAAGTCGACAG+AGG + chr1.2:68869371-68869390 MS.gene60555:CDS 50.0%
GGGAACTTGCCGACGAATTA+AGG + chr1.2:68869392-68869411 MS.gene60555:CDS 50.0%
TGAGATTTGGAGGTGGTTGC+AGG - chr1.2:68868309-68868328 None:intergenic 50.0%
TGGTGGGAAGAGAAGATCCA+AGG - chr1.2:68868209-68868228 None:intergenic 50.0%
TGTTGACGTTGATACCGAGG+CGG + chr1.2:68868544-68868563 MS.gene60555:CDS 50.0%
AGTGGCGTGAGAGAGACGAA+AGG - chr1.2:68868243-68868262 None:intergenic 55.0%
CACCTCCAAATCTCAACCGG+CGG + chr1.2:68868314-68868333 MS.gene60555:CDS 55.0%
GAAAGTGAGAGGAGCGACAG+TGG - chr1.2:68868261-68868280 None:intergenic 55.0%
GAACGATGAAGCCACCTAGC+AGG - chr1.2:68868470-68868489 None:intergenic 55.0%
GACCACGAGCTCAAAGGTGA+AGG + chr1.2:68868416-68868435 MS.gene60555:CDS 55.0%
GATTTGGAGGTGGTTGCAGG+CGG - chr1.2:68868306-68868325 None:intergenic 55.0%
TCCGGGAAAACAGCTGGAGT+CGG - chr1.2:68868575-68868594 None:intergenic 55.0%
TGGCACGCGATTACTCCTTG+CGG + chr1.2:68868353-68868372 MS.gene60555:CDS 55.0%
! GAAGCATCACACCGCTGCTT+CGG - chr1.2:68868391-68868410 None:intergenic 55.0%
!! GGTATAGGGGTGCTTGCTGA+TGG + chr1.2:68869292-68869311 MS.gene60555:intron 55.0%
!! TAGGGGTGCTTGCTGATGGT+CGG + chr1.2:68869296-68869315 MS.gene60555:intron 55.0%
ATTACTCCTTGCGGCGGCGA+TGG + chr1.2:68868362-68868381 MS.gene60555:CDS 60.0%
CAACAATAACCGGCGGCGCA+AGG - chr1.2:68868529-68868548 None:intergenic 60.0%
CACGCGATTACTCCTTGCGG+CGG + chr1.2:68868356-68868375 MS.gene60555:CDS 60.0%
CGTGAGAGAGACGAAAGGCG+TGG - chr1.2:68868238-68868257 None:intergenic 60.0%
CTCCAAATCTCAACCGGCGG+TGG + chr1.2:68868317-68868336 MS.gene60555:CDS 60.0%
CTCTCGTCCGGGAAAACAGC+TGG - chr1.2:68868581-68868600 None:intergenic 60.0%
CTTGGGATGCTCGTCCTGCT+AGG + chr1.2:68868453-68868472 MS.gene60555:CDS 60.0%
GATCCAAGGACGAGAGCGAG+TGG - chr1.2:68868195-68868214 None:intergenic 60.0%
GCGGTGGAGCTGCTTCGATA+TGG + chr1.2:68868333-68868352 MS.gene60555:CDS 60.0%
GGGAAAACAGCTGGAGTCGG+CGG - chr1.2:68868572-68868591 None:intergenic 60.0%
TATCGAAGCAGCTCCACCGC+CGG - chr1.2:68868333-68868352 None:intergenic 60.0%
TTCCCGGACGAGAGTTCCGA+GGG + chr1.2:68868587-68868606 MS.gene60555:CDS 60.0%
TTTCCCGGACGAGAGTTCCG+AGG + chr1.2:68868586-68868605 MS.gene60555:CDS 60.0%
! AGAGACGAAAGGCGTGGTGG+TGG - chr1.2:68868232-68868251 None:intergenic 60.0%
! CACCGCCGGTTGAGATTTGG+AGG - chr1.2:68868319-68868338 None:intergenic 60.0%
! CGCCGGTTGAGATTTGGAGG+TGG - chr1.2:68868316-68868335 None:intergenic 60.0%
! CTCCACCGCCGGTTGAGATT+TGG - chr1.2:68868322-68868341 None:intergenic 60.0%
! GAGAGAGACGAAAGGCGTGG+TGG - chr1.2:68868235-68868254 None:intergenic 60.0%
! GGCGATGGATTCCGAAGCAG+CGG + chr1.2:68868377-68868396 MS.gene60555:CDS 60.0%
!! AAAGGCGTGGTGGTGGTGGT+GGG - chr1.2:68868225-68868244 None:intergenic 60.0%
!! GCCGACTCCAGCTGTTTTCC+CGG + chr1.2:68868571-68868590 MS.gene60555:CDS 60.0%
CGTCCCTCGGAACTCTCGTC+CGG - chr1.2:68868593-68868612 None:intergenic 65.0%
GGGATGCTCGTCCTGCTAGG+TGG + chr1.2:68868456-68868475 MS.gene60555:CDS 65.0%
GGTGGTTGCAGGCGGTGGAA+TGG - chr1.2:68868298-68868317 None:intergenic 65.0%
GGTTGCAGGCGGTGGAATGG+TGG - chr1.2:68868295-68868314 None:intergenic 65.0%
GTCCCTCGGAACTCTCGTCC+GGG - chr1.2:68868592-68868611 None:intergenic 65.0%
TGCAGGCGGTGGAATGGTGG+CGG - chr1.2:68868292-68868311 None:intergenic 65.0%
TTGGAGGTGGTTGCAGGCGG+TGG - chr1.2:68868303-68868322 None:intergenic 65.0%
!! GAAAGGCGTGGTGGTGGTGG+TGG - chr1.2:68868226-68868245 None:intergenic 65.0%
!! GACGAAAGGCGTGGTGGTGG+TGG - chr1.2:68868229-68868248 None:intergenic 65.0%
CGGAATCCATCGCCGCCGCA+AGG - chr1.2:68868371-68868390 None:intergenic 70.0%
GCGCCACTCGCTCTCGTCCT+TGG + chr1.2:68868189-68868208 MS.gene60555:CDS 70.0%
!! AGGCGGTGGAATGGTGGCGG+CGG - chr1.2:68868289-68868308 None:intergenic 70.0%
CGGCGGGCGGGGAAAGTGAG+AGG - chr1.2:68868272-68868291 None:intergenic 75.0%
CTGCGCTTGCCTTGCGCCGC+CGG + chr1.2:68868517-68868536 MS.gene60555:CDS 75.0%
!! GGCGGTGGAATGGTGGCGGC+GGG - chr1.2:68868288-68868307 None:intergenic 75.0%
!! GGTGGAATGGTGGCGGCGGG+CGG - chr1.2:68868285-68868304 None:intergenic 75.0%
!! GTGGAATGGTGGCGGCGGGC+GGG - chr1.2:68868284-68868303 None:intergenic 75.0%
!! TGGAATGGTGGCGGCGGGCG+GGG - chr1.2:68868283-68868302 None:intergenic 75.0%
! TGGAGTCGGCGGCGCCGCCT+CGG - chr1.2:68868561-68868580 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 68868173 68870330 68868173 ID=MS.gene60555
chr1.2 mRNA 68868173 68870330 68868173 ID=MS.gene60555.t1;Parent=MS.gene60555
chr1.2 exon 68868173 68868677 68868173 ID=MS.gene60555.t1.exon1;Parent=MS.gene60555.t1
chr1.2 CDS 68868173 68868677 68868173 ID=cds.MS.gene60555.t1;Parent=MS.gene60555.t1
chr1.2 exon 68869299 68869420 68869299 ID=MS.gene60555.t1.exon2;Parent=MS.gene60555.t1
chr1.2 CDS 68869299 68869420 68869299 ID=cds.MS.gene60555.t1;Parent=MS.gene60555.t1
chr1.2 exon 68869535 68869575 68869535 ID=MS.gene60555.t1.exon3;Parent=MS.gene60555.t1
chr1.2 CDS 68869535 68869575 68869535 ID=cds.MS.gene60555.t1;Parent=MS.gene60555.t1
chr1.2 exon 68869676 68869774 68869676 ID=MS.gene60555.t1.exon4;Parent=MS.gene60555.t1
chr1.2 CDS 68869676 68869774 68869676 ID=cds.MS.gene60555.t1;Parent=MS.gene60555.t1
chr1.2 exon 68870180 68870330 68870180 ID=MS.gene60555.t1.exon5;Parent=MS.gene60555.t1
chr1.2 CDS 68870180 68870330 68870180 ID=cds.MS.gene60555.t1;Parent=MS.gene60555.t1
Gene Sequence

>MS.gene60555

ATGCTTGGTGTACTCTGCGCCACTCGCTCTCGTCCTTGGATCTTCTCTTCCCACCACCACCACCACGCCTTTCGTCTCTCTCACGCCACTGTCGCTCCTCTCACTTTCCCCGCCCGCCGCCACCATTCCACCGCCTGCAACCACCTCCAAATCTCAACCGGCGGTGGAGCTGCTTCGATATGGCACGCGATTACTCCTTGCGGCGGCGATGGATTCCGAAGCAGCGGTGTGATGCTTCATCATGACCACGAGCTCAAAGGTGAAGGATCGTGGAACGTTGCTTGGGATGCTCGTCCTGCTAGGTGGCTTCATCGTTCTGACTCAGCTTGGCTTCTTTTCGGTGTCTGCGCTTGCCTTGCGCCGCCGGTTATTGTTGACGTTGATACCGAGGCGGCGCCGCCGACTCCAGCTGTTTTCCCGGACGAGAGTTCCGAGGGACGTGAAGTGAAAGATGAGCTTTCCGATGAACGTGACGATGAACTCAACGCTGATTACAGAGTCACGGGTTCGTTTAATTTTTTTTTATTTCGTAAAAGTGTTTAATCATAATCTTTCTCTTTGTGTGTGCGTTAATAACGTGTTTAATCATAATTTGTTTATGAACAGATTTTACTTTGAGAAAAACAAACAACTTTATAAAAGATGAATACTTTATGAAAAATAATATTTCATTTTTTGTCTTTAATGGATATTTTTTTTTCATATAAGTACAGGGATTGTTTGGTTTGATGATATTTGTGCTGATTTTGTTATTGTGTCAACATAGTGTCATCAAACTTACTAAAATAATACTCTTTTTGTTTGTGACCAAGTGTTTGATGGTTTGTCACAATGCAACTTTACAACTACGAAACAAGAACGGACATCAGACACAACATTGACACAGACACAACATTGACACGTGTATATTCAAAATATTTTTAAAAAATAAAAGTAATCCAATGATACAATAGTTCTTGTTAGTGTCCAACTCAAGACACTTGTTCAATATGATCTGTCGGTGCTCACTGTTTATGTTGTTACGTTGTCATTGTTCAGTTATTTTGATGGTGTGTTTAGTAAGTAATAAACATGAGTTAGAGATTATGTGGGTTCATTTATAAGTTTGTATCACTGAATGGTATAGGGGTGCTTGCTGATGGTCGGTGTCTGTTCAGAGCAATAGCTCATGGAGCTTGCTTGAAAAATGGAGAGGAAGCGCCTAATGAAAGTCGACAGAGGGAACTTGCCGACGAATTAAGGGTTAAAGTAAGATCATCTTGCTAGAACTTTTGAAACGGAGAGGCATTCCTTCTTCTTTGTCAACATTCTTTTTTTCCTTCAATGAATTTTGTTACCCAATTTTTGTGATGTTTTATTTAGGTTGCTGAAGAACTTTTGAACAGGCGCAAGGAAACAGAATGGTGAGCTTTGTTATTTGAAGGAAACTACATACAATTATTTGGTTTTTATTTTACTTTTATTTACATGGTAAATTGGTTGTTATATTTGCTGTGTCTATAGGTTTATAGAAGGGGATTTTGACACATATGTGACCAGAATTCAACAGACTTATGTTTGGGGAGGAGAGCCTGAGCTGTTAATGGCTTCTCATGTTTTGAAGTGAGTTTTGATGAATTAACTTTCTTGTTATTTTCTAGTTTCAGTTACATTTGATTGATGATTGACGATGACTGTTAAAGTTTAAATTTTGATGATGTTGTAGCATACACATTAACAATTTTACACAAACAATAAAACATCATATCCTACAACAACAGTTTAACTTGTTGTGGTACTTTTCAGAACATACATATTAGTAGCATAATGATAATCGATGGATATTAATCTACGGGTCAAACAATGAGCATGATACTAAAATTTGAAAACTTATAGAGAAACAAATTTCCGGTTCATTTTGTGGTAAATCGTTGGGACTTGGGAGTCATGTGAAATCTAATATTCGTACTATTTTTATATATGTTATTCCATTTCGTTGTACCTAACATTGTCTTCATAACTTACAAGGACTCCAATATATGTCTTCATGAGAGATGCAAGCTCCATGGATTTGGTAAATATAGCAAAATACGGTGAAGAATATATGAATGATGAGGAGATTTCCATCAATGTGCTATTTCATCGACATGGTCACTACGAACTATTGGAGACCTTATGA
Protein sequence

>MS.gene60555.t1

MLGVLCATRSRPWIFSSHHHHHAFRLSHATVAPLTFPARRHHSTACNHLQISTGGGAASIWHAITPCGGDGFRSSGVMLHHDHELKGEGSWNVAWDARPARWLHRSDSAWLLFGVCACLAPPVIVDVDTEAAPPTPAVFPDESSEGREVKDELSDERDDELNADYRVTGVLADGRCLFRAIAHGACLKNGEEAPNESRQRELADELRVKVAEELLNRRKETEWFIEGDFDTYVTRIQQTYVWGGEPELLMASHVLKTPIYVFMRDASSMDLVNIAKYGEEYMNDEEISINVLFHRHGHYELLETL