AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene60557

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60557.t1 MTR_1g492790 97.233 506 8 3 1 505 1 501 0.0 1024
MS.gene60557.t1 MTR_1g060990 39.012 405 186 16 10 402 27 382 6.45e-74 243
MS.gene60557.t1 MTR_1g098900 32.665 349 178 12 75 383 24 355 5.53e-42 159
MS.gene60557.t1 MTR_1g098900 40.541 111 62 3 98 207 436 543 8.68e-19 90.1
MS.gene60557.t1 MTR_1g098870 30.969 423 218 11 99 476 36 429 2.10e-35 139
MS.gene60557.t1 MTR_1g098870 47.525 101 51 2 110 209 364 463 4.50e-19 90.9
MS.gene60557.t1 MTR_6g035370 61.957 92 34 1 117 208 54 144 2.43e-31 128
MS.gene60557.t1 MTR_6g035370 45.652 92 48 2 114 204 460 550 1.13e-18 89.7
MS.gene60557.t1 MTR_2g026725 27.193 228 116 8 307 492 114 333 2.60e-14 75.1
MS.gene60557.t1 MTR_2g026725 39.785 93 54 2 113 204 257 348 2.74e-13 72.0
MS.gene60557.t1 MTR_7g114860 41.237 97 46 3 118 204 381 476 2.07e-13 72.8
MS.gene60557.t1 MTR_1g081180 45.122 82 42 2 110 189 410 490 6.55e-12 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60557.t1 AT5G03680 36.699 515 252 16 5 481 9 487 7.67e-86 276
MS.gene60557.t1 AT3G10000 39.071 366 191 12 40 399 15 354 3.21e-65 219
MS.gene60557.t1 AT3G10000 37.158 366 188 13 40 399 15 344 8.21e-57 196
MS.gene60557.t1 AT1G76890 31.696 448 215 12 114 488 37 466 1.01e-49 179
MS.gene60557.t1 AT1G76890 40.000 160 78 8 88 233 360 515 1.87e-19 92.0
MS.gene60557.t1 AT1G76880 54.701 117 46 2 97 213 46 155 9.62e-31 126
MS.gene60557.t1 AT1G76880 45.361 97 51 2 114 209 404 499 8.38e-18 87.0
MS.gene60557.t1 AT1G33240 44.318 88 47 2 118 204 435 521 2.76e-15 79.3
MS.gene60557.t1 AT1G33240 44.318 88 47 2 118 204 435 521 3.18e-15 79.0
MS.gene60557.t1 AT1G33240 44.318 88 47 2 118 204 396 482 4.15e-15 78.6
MS.gene60557.t1 AT5G28300 39.216 102 50 4 118 208 459 559 5.12e-13 72.0

Find 106 sgRNAs with CRISPR-Local

Find 149 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTGGCTCTTACTCTTATT+TGG 0.155961 1.2:+68914324 None:intergenic
TTGTTGTATCATTTGCTTCT+TGG 0.177243 1.2:+68914307 None:intergenic
TGCATTTCTTTCCACTTCTT+TGG 0.203428 1.2:+68915015 None:intergenic
AGGATAATGGCTGAGGAATT+TGG 0.204963 1.2:-68915041 MS.gene60557:CDS
CCTTGTGGAATGAAATTTCT+AGG 0.236619 1.2:-68916058 MS.gene60557:intron
GAACATCAAGTAAGAGAAAA+AGG 0.239292 1.2:-68914195 MS.gene60557:CDS
AGGTGAAAGGATGGTTTATA+TGG 0.259177 1.2:-68914142 MS.gene60557:CDS
GATATTTGGATGATGGAGTT+TGG 0.262012 1.2:-68914173 MS.gene60557:CDS
AAACACAAGGCCTTGGTGTT+TGG 0.270837 1.2:-68914034 MS.gene60557:CDS
GAGCTGAGTGCAAGAAAATA+TGG 0.284773 1.2:-68914104 MS.gene60557:CDS
AACACAAGGCCTTGGTGTTT+GGG 0.305639 1.2:-68914033 MS.gene60557:CDS
TCTACTAGCTTTACCTTCCT+TGG 0.314459 1.2:+68914953 None:intergenic
GATCTTCTTGTTTAGGATAA+TGG 0.314988 1.2:-68915054 MS.gene60557:intron
TGGTTTATATGGGATTTAAT+AGG 0.331668 1.2:-68914131 MS.gene60557:CDS
ATAGGGTCATAGGAATAATT+TGG 0.333734 1.2:+68916371 None:intergenic
ATCAAGAAATACATGAACTT+TGG 0.336932 1.2:-68914431 MS.gene60557:CDS
GGATAATGGCTGAGGAATTT+GGG 0.344397 1.2:-68915040 MS.gene60557:CDS
AAAACATCACCAACTATCAT+AGG 0.369956 1.2:+68916353 None:intergenic
GAGAAGATGAGTGACCTATA+TGG 0.374450 1.2:-68916500 None:intergenic
ATATTTGGATGATGGAGTTT+GGG 0.376772 1.2:-68914172 MS.gene60557:CDS
GATGGAGTTTGGGATGAAAT+AGG 0.383462 1.2:-68914162 MS.gene60557:CDS
TCAAGAAATACATGAACTTT+GGG 0.384682 1.2:-68914430 MS.gene60557:CDS
GTAGCAACAAAGGAAGCATT+AGG 0.385971 1.2:+68914834 None:intergenic
GCAGCTATAGTGGTATTTGT+AGG 0.401800 1.2:+68916278 None:intergenic
GAAGTTGTAAGAAAACATAT+TGG 0.406391 1.2:-68914363 MS.gene60557:CDS
GGTTTAGAAACTGCTGATAA+AGG 0.416775 1.2:-68916197 MS.gene60557:CDS
TCCTATGACCCTATGATAGT+TGG 0.417353 1.2:-68916362 MS.gene60557:CDS
AAGTTGTAAGAAAACATATT+GGG 0.418479 1.2:-68914362 MS.gene60557:CDS
TAGCAACAAAGGAAGCATTA+GGG 0.434533 1.2:+68914835 None:intergenic
GAGTGACCTATATGGGTTAC+CGG 0.435419 1.2:-68916492 MS.gene60557:CDS
GAGGCATGAGAGTTTGTGTT+TGG 0.435521 1.2:+68914876 None:intergenic
GATCCTCCGGTAACCCATAT+AGG 0.435631 1.2:+68916486 None:intergenic
AGAAAGGGCAAAGTGGTCAT+AGG 0.438060 1.2:-68914629 MS.gene60557:CDS
ATATTAGTATGTCTCTTAGA+AGG 0.438578 1.2:-68914077 MS.gene60557:CDS
ATCAACCGCCGGAGATCCTC+CGG 0.444001 1.2:+68916473 None:intergenic
TATATGGGATTTAATAGGAA+TGG 0.452119 1.2:-68914126 MS.gene60557:CDS
ACCAACTATCATAGGGTCAT+AGG 0.456713 1.2:+68916361 None:intergenic
TGCAGCAGCAGCAGCTATAG+TGG 0.457554 1.2:+68916268 None:intergenic
ATATTATCCACACTTTCAAA+AGG 0.458934 1.2:+68914273 None:intergenic
GCTTCTACTGCATTCTGTGT+AGG 0.459654 1.2:-68916218 MS.gene60557:CDS
TATTCATAGATGGACAGAAA+TGG 0.466542 1.2:-68914253 MS.gene60557:CDS
GAACCACCACCTTGTTTGTA+TGG 0.467332 1.2:-68916416 MS.gene60557:CDS
AGAACAAGAGATGGCATCAA+AGG 0.471241 1.2:-68914490 MS.gene60557:CDS
TTGATTCTTGTAACAACAGA+TGG 0.472038 1.2:-68916160 MS.gene60557:CDS
TTTCAAACTTGATACAACTA+AGG 0.475487 1.2:-68914227 MS.gene60557:CDS
AGAAGATGAGTGACCTATAT+GGG 0.480823 1.2:-68916499 None:intergenic
ATTAACCAAGAAAATACTAA+TGG 0.483532 1.2:-68914792 MS.gene60557:CDS
TTATTGAGACTCAAGATGCT+TGG 0.485535 1.2:-68914545 MS.gene60557:CDS
ATGAAGTCGTCGAAGGAGAA+AGG 0.490837 1.2:-68914645 MS.gene60557:CDS
GGTGAAAGGATGGTTTATAT+GGG 0.497182 1.2:-68914141 MS.gene60557:CDS
AAACAAGGTGGTGGTTCAGC+TGG 0.498191 1.2:+68916422 None:intergenic
GGATGAAATAGGTGAAAGGA+TGG 0.499715 1.2:-68914151 MS.gene60557:CDS
GTGTGGATAATATTCATAGA+TGG 0.500320 1.2:-68914263 MS.gene60557:CDS
CTTGTTTAGGATAATGGCTG+AGG 0.502416 1.2:-68915048 MS.gene60557:intron
ATAGGAGGGCAAGGAAAAGT+TGG 0.504848 1.2:-68914611 MS.gene60557:CDS
TATTATCCACACTTTCAAAA+GGG 0.515340 1.2:+68914274 None:intergenic
ACTGCTGATAAAGGATGGCT+TGG 0.518070 1.2:-68916188 MS.gene60557:CDS
ATGGGTTACCGGAGGATCTC+CGG 0.518191 1.2:-68916481 MS.gene60557:CDS
CAGCTATAGTGGTATTTGTA+GGG 0.519351 1.2:+68916279 None:intergenic
TGAGACTCAAGATGCTTGGA+TGG 0.522893 1.2:-68914541 MS.gene60557:CDS
AGACAGCTTGAAGCAATATG+TGG 0.533898 1.2:-68914903 MS.gene60557:CDS
TGAAGTCGTCGAAGGAGAAA+GGG 0.536001 1.2:-68914644 MS.gene60557:CDS
TGGCATCAAAGGAAGAAGAA+AGG 0.537348 1.2:-68914479 MS.gene60557:CDS
TTAGACTTTGTGAATACTTG+TGG 0.538073 1.2:+68914745 None:intergenic
GGAAGAGAAAAGAGTCAATG+AGG 0.550829 1.2:-68914458 MS.gene60557:CDS
AGAGAAAGAGTCTCTTGTCT+AGG 0.553492 1.2:+68916137 None:intergenic
TAGAAACTGCTGATAAAGGA+TGG 0.556731 1.2:-68916193 MS.gene60557:CDS
AAACATCACCAACTATCATA+GGG 0.561437 1.2:+68916354 None:intergenic
TTGAGTTTGAGTAGCAACAA+AGG 0.562015 1.2:+68914824 None:intergenic
AAGGGCAAAGTGGTCATAGG+AGG 0.562963 1.2:-68914626 MS.gene60557:CDS
GGTATTTGTAGGGTTAACAG+TGG 0.564956 1.2:+68916289 None:intergenic
GGTAAAGCTAGTAGACAAGA+TGG 0.568681 1.2:-68914945 MS.gene60557:CDS
CCTAGAAATTTCATTCCACA+AGG 0.573860 1.2:+68916058 None:intergenic
TCATAGGAATAATTTGGTGG+AGG 0.577820 1.2:+68916377 None:intergenic
GAATTTGGGTACCAAAGAAG+TGG 0.582190 1.2:-68915026 MS.gene60557:CDS
CAGTGGAGGAATAGTCATAG+TGG 0.586600 1.2:+68916306 None:intergenic
TTGATGATGTTATCAACCGC+CGG 0.588660 1.2:+68916462 None:intergenic
AGGGCAAGGAAAAGTTGGAG+AGG 0.591096 1.2:-68914606 MS.gene60557:CDS
ATTTGTAGGGTTAACAGTGG+AGG 0.594970 1.2:+68916292 None:intergenic
AGCTGAGTGCAAGAAAATAT+GGG 0.595363 1.2:-68914103 MS.gene60557:CDS
TTTGGGATGAAATAGGTGAA+AGG 0.600563 1.2:-68914155 MS.gene60557:CDS
TATTACAAGAAGACCAAGGA+AGG 0.601931 1.2:-68914966 MS.gene60557:CDS
GGGTCATAGGAATAATTTGG+TGG 0.603660 1.2:+68916374 None:intergenic
AAGTTGGAGAGGTAAAGTTG+AGG 0.607935 1.2:-68914595 MS.gene60557:CDS
ATAATCAGAAAGCACCCTTG+TGG 0.619380 1.2:-68916073 MS.gene60557:CDS
AAAGTGGTCATAGGAGGGCA+AGG 0.623960 1.2:-68914620 MS.gene60557:CDS
GGTGAGTGAAGCTACTACGA+GGG 0.624158 1.2:+68916327 None:intergenic
TGAACAAAGAGAACAAGAGA+TGG 0.625612 1.2:-68914499 MS.gene60557:CDS
AGCAACAAAGGAAGCATTAG+GGG 0.629023 1.2:+68914836 None:intergenic
CTTAGAAGGACAGTGGACTG+TGG 0.637596 1.2:-68914063 MS.gene60557:CDS
AAGAAGTGGAAAGAAATGCA+AGG 0.641850 1.2:-68915012 MS.gene60557:CDS
AGGGCAAAGTGGTCATAGGA+GGG 0.643035 1.2:-68914625 MS.gene60557:CDS
ACACACTATGAAGTCGTCGA+AGG 0.660577 1.2:-68914652 MS.gene60557:CDS
TATGTCTCTTAGAAGGACAG+TGG 0.662720 1.2:-68914070 MS.gene60557:CDS
GCACCATACAAACAAGGTGG+TGG 0.666201 1.2:+68916413 None:intergenic
CTCATCTGAAAACAACGATG+AGG 0.668787 1.2:-68914691 MS.gene60557:CDS
AGCTTAAGTCCCAAACACCA+AGG 0.671681 1.2:+68914024 None:intergenic
AACTTGATACAACTAAGGAG+TGG 0.671997 1.2:-68914222 MS.gene60557:CDS
TCGAAGGAGAAAGGGCAAAG+TGG 0.682624 1.2:-68914636 MS.gene60557:CDS
GCAGCAGCACCATACAAACA+AGG 0.705312 1.2:+68916407 None:intergenic
CAAGTATTACAAGAAGACCA+AGG 0.709647 1.2:-68914970 MS.gene60557:CDS
CTAGAAATTTCATTCCACAA+GGG 0.711484 1.2:+68916059 None:intergenic
TGACCTATATGGGTTACCGG+AGG 0.716927 1.2:-68916489 MS.gene60557:CDS
TGGTGAGTGAAGCTACTACG+AGG 0.746302 1.2:+68916326 None:intergenic
GGTTACCGGAGGATCTCCGG+CGG 0.758338 1.2:-68916478 MS.gene60557:CDS
GCAGCACCATACAAACAAGG+TGG 0.760932 1.2:+68916410 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAATTTAAATTGTAGTTA+CGG - chr1.2:68915141-68915160 MS.gene60557:intron 10.0%
!!! TTTATTTCACTTTTTTTTTT+TGG - chr1.2:68914502-68914521 MS.gene60557:CDS 10.0%
!! ATCATAATAAAAAAAACACA+TGG + chr1.2:68914615-68914634 None:intergenic 15.0%
!! AAGTTGTAAGAAAACATATT+GGG - chr1.2:68916134-68916153 MS.gene60557:CDS 20.0%
!! ATTAACCAAGAAAATACTAA+TGG - chr1.2:68915704-68915723 MS.gene60557:intron 20.0%
!! GACAAAAAAGAAAATTATCA+TGG + chr1.2:68914567-68914586 None:intergenic 20.0%
!! TTAGTGTCTTAATTAATTAC+TGG - chr1.2:68914467-68914486 MS.gene60557:CDS 20.0%
!!! TTTTGTTTGAAAATATCATG+TGG - chr1.2:68914976-68914995 MS.gene60557:CDS 20.0%
! AGTAAGAGAAAAAGGATATT+TGG - chr1.2:68916309-68916328 MS.gene60557:CDS 25.0%
! ATATTAGTATGTCTCTTAGA+AGG - chr1.2:68916419-68916438 MS.gene60557:CDS 25.0%
! ATATTATCCACACTTTCAAA+AGG + chr1.2:68916226-68916245 None:intergenic 25.0%
! ATCAAGAAATACATGAACTT+TGG - chr1.2:68916065-68916084 MS.gene60557:CDS 25.0%
! CAATGAATGATTTAGTATCA+TGG + chr1.2:68914710-68914729 None:intergenic 25.0%
! GAAGTTGTAAGAAAACATAT+TGG - chr1.2:68916133-68916152 MS.gene60557:CDS 25.0%
! TATATGGGATTTAATAGGAA+TGG - chr1.2:68916370-68916389 MS.gene60557:CDS 25.0%
! TATTATCCACACTTTCAAAA+GGG + chr1.2:68916225-68916244 None:intergenic 25.0%
! TCAAGAAATACATGAACTTT+GGG - chr1.2:68916066-68916085 MS.gene60557:CDS 25.0%
! TGGTTTATATGGGATTTAAT+AGG - chr1.2:68916365-68916384 MS.gene60557:CDS 25.0%
! TTTCAAACTTGATACAACTA+AGG - chr1.2:68916269-68916288 MS.gene60557:CDS 25.0%
!! ATTTTCTTGGTTAATTGTGA+AGG + chr1.2:68915699-68915718 None:intergenic 25.0%
AAAACATCACCAACTATCAT+AGG + chr1.2:68914146-68914165 None:intergenic 30.0%
AAACATCACCAACTATCATA+GGG + chr1.2:68914145-68914164 None:intergenic 30.0%
AAATCAAGTGATACTGTGAT+AGG + chr1.2:68914952-68914971 None:intergenic 30.0%
ATAGGGTCATAGGAATAATT+TGG + chr1.2:68914128-68914147 None:intergenic 30.0%
CGTATATACAAAACAAAGAC+AGG + chr1.2:68914766-68914785 None:intergenic 30.0%
CTAGAAATTTCATTCCACAA+GGG + chr1.2:68914440-68914459 None:intergenic 30.0%
GAAAAAGGATATTTGGATGA+TGG - chr1.2:68916316-68916335 MS.gene60557:CDS 30.0%
GAACATCAAGTAAGAGAAAA+AGG - chr1.2:68916301-68916320 MS.gene60557:CDS 30.0%
GATACTAAATCATTCATTGC+AGG - chr1.2:68914711-68914730 MS.gene60557:CDS 30.0%
GTGATTGTAATTGAAACCTT+GGG + chr1.2:68915034-68915053 None:intergenic 30.0%
GTGTGGATAATATTCATAGA+TGG - chr1.2:68916233-68916252 MS.gene60557:CDS 30.0%
TATTCATAGATGGACAGAAA+TGG - chr1.2:68916243-68916262 MS.gene60557:CDS 30.0%
TGATTGTAATTGAAACCTTG+GGG + chr1.2:68915033-68915052 None:intergenic 30.0%
TGTGATTGTAATTGAAACCT+TGG + chr1.2:68915035-68915054 None:intergenic 30.0%
TTAGACTTTGTGAATACTTG+TGG + chr1.2:68915754-68915773 None:intergenic 30.0%
TTGAAAATATCATGTGGTCT+TGG - chr1.2:68914982-68915001 MS.gene60557:CDS 30.0%
TTGATTCTTGTAACAACAGA+TGG - chr1.2:68914336-68914355 MS.gene60557:CDS 30.0%
TTGTTGTATCATTTGCTTCT+TGG + chr1.2:68916192-68916211 None:intergenic 30.0%
! CAACACCATTAGTATTTTCT+TGG + chr1.2:68915712-68915731 None:intergenic 30.0%
! CTTTTGTGATTGAAAATGAC+AGG - chr1.2:68914534-68914553 MS.gene60557:CDS 30.0%
! GATCTTCTTGTTTAGGATAA+TGG - chr1.2:68915442-68915461 MS.gene60557:intron 30.0%
! TGTTAGTGTTTTGTACTGAA+AGG + chr1.2:68914918-68914937 None:intergenic 30.0%
!! ATATTTGGATGATGGAGTTT+GGG - chr1.2:68916324-68916343 MS.gene60557:CDS 30.0%
!! GGTAATTTTGTCTAAAGTTG+AGG + chr1.2:68915642-68915661 None:intergenic 30.0%
!!! CTGTCATTTTCAATCACAAA+AGG + chr1.2:68914536-68914555 None:intergenic 30.0%
AACTTGATACAACTAAGGAG+TGG - chr1.2:68916274-68916293 MS.gene60557:CDS 35.0%
AAGAAGTGGAAAGAAATGCA+AGG - chr1.2:68915484-68915503 MS.gene60557:intron 35.0%
AGCTGAGTGCAAGAAAATAT+GGG - chr1.2:68916393-68916412 MS.gene60557:CDS 35.0%
AGGTGAAAGGATGGTTTATA+TGG - chr1.2:68916354-68916373 MS.gene60557:CDS 35.0%
CAAGTATTACAAGAAGACCA+AGG - chr1.2:68915526-68915545 MS.gene60557:intron 35.0%
CAGCTATAGTGGTATTTGTA+GGG + chr1.2:68914220-68914239 None:intergenic 35.0%
CCTAGAAATTTCATTCCACA+AGG + chr1.2:68914441-68914460 None:intergenic 35.0%
CCTTGTGGAATGAAATTTCT+AGG - chr1.2:68914438-68914457 MS.gene60557:CDS 35.0%
GCACAAAGCTGTAATAAATG+TGG - chr1.2:68915250-68915269 MS.gene60557:intron 35.0%
GGTGAAAGGATGGTTTATAT+GGG - chr1.2:68916355-68916374 MS.gene60557:CDS 35.0%
GGTTTAGAAACTGCTGATAA+AGG - chr1.2:68914299-68914318 MS.gene60557:CDS 35.0%
TAGAAACTGCTGATAAAGGA+TGG - chr1.2:68914303-68914322 MS.gene60557:CDS 35.0%
TATCCCACAAATCTTTGCAT+TGG - chr1.2:68915079-68915098 MS.gene60557:intron 35.0%
TATTACAAGAAGACCAAGGA+AGG - chr1.2:68915530-68915549 MS.gene60557:intron 35.0%
TGAACAAAGAGAACAAGAGA+TGG - chr1.2:68915997-68916016 MS.gene60557:intron 35.0%
TGCATTTCTTTCCACTTCTT+TGG + chr1.2:68915484-68915503 None:intergenic 35.0%
TTATTGAGACTCAAGATGCT+TGG - chr1.2:68915951-68915970 MS.gene60557:intron 35.0%
TTCATTGCAGGTGCAAATTA+AGG - chr1.2:68914723-68914742 MS.gene60557:CDS 35.0%
TTGAGTTTGAGTAGCAACAA+AGG + chr1.2:68915675-68915694 None:intergenic 35.0%
TTTGGGCTAAAGAAAAAGCA+TGG - chr1.2:68916083-68916102 MS.gene60557:CDS 35.0%
! GCTTTGTGATCTTCTTGTTT+AGG - chr1.2:68915435-68915454 MS.gene60557:intron 35.0%
! TAGTACAACTTTTTACCCCA+AGG - chr1.2:68915015-68915034 MS.gene60557:CDS 35.0%
! TCATAGGAATAATTTGGTGG+AGG + chr1.2:68914122-68914141 None:intergenic 35.0%
! TCTTGGCTCTTACTCTTATT+TGG + chr1.2:68916175-68916194 None:intergenic 35.0%
! TTGGGCTAAAGAAAAAGCAT+GGG - chr1.2:68916084-68916103 MS.gene60557:CDS 35.0%
! TTTGGGATGAAATAGGTGAA+AGG - chr1.2:68916341-68916360 MS.gene60557:CDS 35.0%
!! GATATTTGGATGATGGAGTT+TGG - chr1.2:68916323-68916342 MS.gene60557:CDS 35.0%
!! TAGCAACAAAGGAAGCATTA+GGG + chr1.2:68915664-68915683 None:intergenic 35.0%
!! TGAATACCCTTTTGAAAGTG+TGG - chr1.2:68916216-68916235 MS.gene60557:CDS 35.0%
AAGTTGGAGAGGTAAAGTTG+AGG - chr1.2:68915901-68915920 MS.gene60557:intron 40.0%
ACCAACTATCATAGGGTCAT+AGG + chr1.2:68914138-68914157 None:intergenic 40.0%
ACTGTGGAGTCAAAAACACA+AGG - chr1.2:68916449-68916468 MS.gene60557:CDS 40.0%
AGAACAAGAGATGGCATCAA+AGG - chr1.2:68916006-68916025 MS.gene60557:intron 40.0%
AGACAGCTTGAAGCAATATG+TGG - chr1.2:68915593-68915612 MS.gene60557:intron 40.0%
AGAGAAAGAGTCTCTTGTCT+AGG + chr1.2:68914362-68914381 None:intergenic 40.0%
ATTTGTAGGGTTAACAGTGG+AGG + chr1.2:68914207-68914226 None:intergenic 40.0%
CCTCCAATGCAAAGATTTGT+GGG + chr1.2:68915085-68915104 None:intergenic 40.0%
CTCATCTGAAAACAACGATG+AGG - chr1.2:68915805-68915824 MS.gene60557:intron 40.0%
GAATTTGGGTACCAAAGAAG+TGG - chr1.2:68915470-68915489 MS.gene60557:intron 40.0%
GAGCTGAGTGCAAGAAAATA+TGG - chr1.2:68916392-68916411 MS.gene60557:CDS 40.0%
GCAGCTATAGTGGTATTTGT+AGG + chr1.2:68914221-68914240 None:intergenic 40.0%
GCTGAGCGAATTAGGTTAAT+AGG + chr1.2:68914795-68914814 None:intergenic 40.0%
GGAAGAGAAAAGAGTCAATG+AGG - chr1.2:68916038-68916057 MS.gene60557:intron 40.0%
GGATGAAATAGGTGAAAGGA+TGG - chr1.2:68916345-68916364 MS.gene60557:CDS 40.0%
GGGTCATAGGAATAATTTGG+TGG + chr1.2:68914125-68914144 None:intergenic 40.0%
GGTAAAGCTAGTAGACAAGA+TGG - chr1.2:68915551-68915570 MS.gene60557:intron 40.0%
GGTATTTGTAGGGTTAACAG+TGG + chr1.2:68914210-68914229 None:intergenic 40.0%
GTGACTATACTTACTTGCAC+TGG - chr1.2:68914677-68914696 MS.gene60557:CDS 40.0%
TATGTCTCTTAGAAGGACAG+TGG - chr1.2:68916426-68916445 MS.gene60557:CDS 40.0%
TCCTATGACCCTATGATAGT+TGG - chr1.2:68914134-68914153 MS.gene60557:CDS 40.0%
TCCTCCAATGCAAAGATTTG+TGG + chr1.2:68915086-68915105 None:intergenic 40.0%
TGGCATCAAAGGAAGAAGAA+AGG - chr1.2:68916017-68916036 MS.gene60557:intron 40.0%
TTGCATTGGAGGAGATCAAA+GGG - chr1.2:68915093-68915112 MS.gene60557:intron 40.0%
TTTGCATTGGAGGAGATCAA+AGG - chr1.2:68915092-68915111 MS.gene60557:intron 40.0%
! AGGATAATGGCTGAGGAATT+TGG - chr1.2:68915455-68915474 MS.gene60557:intron 40.0%
! CTTGTTTAGGATAATGGCTG+AGG - chr1.2:68915448-68915467 MS.gene60557:intron 40.0%
! GATGGAGTTTGGGATGAAAT+AGG - chr1.2:68916334-68916353 MS.gene60557:CDS 40.0%
! GGATAATGGCTGAGGAATTT+GGG - chr1.2:68915456-68915475 MS.gene60557:intron 40.0%
!! AGCAACAAAGGAAGCATTAG+GGG + chr1.2:68915663-68915682 None:intergenic 40.0%
!! ATAATCAGAAAGCACCCTTG+TGG - chr1.2:68914423-68914442 MS.gene60557:CDS 40.0%
!! CTCATCGTTGTTTTCAGATG+AGG + chr1.2:68915807-68915826 None:intergenic 40.0%
!! GTAGCAACAAAGGAAGCATT+AGG + chr1.2:68915665-68915684 None:intergenic 40.0%
!! TCTACTAGCTTTACCTTCCT+TGG + chr1.2:68915546-68915565 None:intergenic 40.0%
!! TTGATGATGTTATCAACCGC+CGG + chr1.2:68914037-68914056 None:intergenic 40.0%
AAACTCCAATAGCCCTAGCT+AGG + chr1.2:68914820-68914839 None:intergenic 45.0%
ACACACTATGAAGTCGTCGA+AGG - chr1.2:68915844-68915863 MS.gene60557:intron 45.0%
ACTGCTGATAAAGGATGGCT+TGG - chr1.2:68914308-68914327 MS.gene60557:CDS 45.0%
AGAAAGGGCAAAGTGGTCAT+AGG - chr1.2:68915867-68915886 MS.gene60557:intron 45.0%
AGCTTAAGTCCCAAACACCA+AGG + chr1.2:68916475-68916494 None:intergenic 45.0%
ATAGGAGGGCAAGGAAAAGT+TGG - chr1.2:68915885-68915904 MS.gene60557:intron 45.0%
ATGAAGTCGTCGAAGGAGAA+AGG - chr1.2:68915851-68915870 MS.gene60557:intron 45.0%
CAGTGGAGGAATAGTCATAG+TGG + chr1.2:68914193-68914212 None:intergenic 45.0%
CCCACAAATCTTTGCATTGG+AGG - chr1.2:68915082-68915101 MS.gene60557:intron 45.0%
GAGGCATGAGAGTTTGTGTT+TGG + chr1.2:68915623-68915642 None:intergenic 45.0%
GAGTCAAAAACACAAGGCCT+TGG - chr1.2:68916455-68916474 MS.gene60557:CDS 45.0%
GCTTCTACTGCATTCTGTGT+AGG - chr1.2:68914278-68914297 MS.gene60557:CDS 45.0%
TGAAGTCGTCGAAGGAGAAA+GGG - chr1.2:68915852-68915871 MS.gene60557:intron 45.0%
TGAGACTCAAGATGCTTGGA+TGG - chr1.2:68915955-68915974 MS.gene60557:intron 45.0%
! GAACCACCACCTTGTTTGTA+TGG - chr1.2:68914080-68914099 MS.gene60557:CDS 45.0%
! GAGTGACCTATATGGGTTAC+CGG - chr1.2:68914004-68914023 MS.gene60557:CDS 45.0%
!! AAACACAAGGCCTTGGTGTT+TGG - chr1.2:68916462-68916481 MS.gene60557:CDS 45.0%
!! AACACAAGGCCTTGGTGTTT+GGG - chr1.2:68916463-68916482 MS.gene60557:CDS 45.0%
AAAGTGGTCATAGGAGGGCA+AGG - chr1.2:68915876-68915895 MS.gene60557:intron 50.0%
AAGGGCAAAGTGGTCATAGG+AGG - chr1.2:68915870-68915889 MS.gene60557:intron 50.0%
AGCTACCTAGCTAGGGCTAT+TGG - chr1.2:68914812-68914831 MS.gene60557:CDS 50.0%
AGGGCAAAGTGGTCATAGGA+GGG - chr1.2:68915871-68915890 MS.gene60557:intron 50.0%
AGGGCAAGGAAAAGTTGGAG+AGG - chr1.2:68915890-68915909 MS.gene60557:intron 50.0%
ATTCGCTCAGCTACCTAGCT+AGG - chr1.2:68914804-68914823 MS.gene60557:CDS 50.0%
GATCCTCCGGTAACCCATAT+AGG + chr1.2:68914013-68914032 None:intergenic 50.0%
GCACCATACAAACAAGGTGG+TGG + chr1.2:68914086-68914105 None:intergenic 50.0%
GCAGCACCATACAAACAAGG+TGG + chr1.2:68914089-68914108 None:intergenic 50.0%
GCAGCAGCACCATACAAACA+AGG + chr1.2:68914092-68914111 None:intergenic 50.0%
GCTAGGTAGCTGAGCGAATT+AGG + chr1.2:68914803-68914822 None:intergenic 50.0%
GGTGAGTGAAGCTACTACGA+GGG + chr1.2:68914172-68914191 None:intergenic 50.0%
TCGAAGGAGAAAGGGCAAAG+TGG - chr1.2:68915860-68915879 MS.gene60557:intron 50.0%
TGGTGAGTGAAGCTACTACG+AGG + chr1.2:68914173-68914192 None:intergenic 50.0%
TTCGCTCAGCTACCTAGCTA+GGG - chr1.2:68914805-68914824 MS.gene60557:CDS 50.0%
! AAACAAGGTGGTGGTTCAGC+TGG + chr1.2:68914077-68914096 None:intergenic 50.0%
! TGACCTATATGGGTTACCGG+AGG - chr1.2:68914007-68914026 MS.gene60557:CDS 50.0%
!! CTTAGAAGGACAGTGGACTG+TGG - chr1.2:68916433-68916452 MS.gene60557:CDS 50.0%
TGCAGCAGCAGCAGCTATAG+TGG + chr1.2:68914231-68914250 None:intergenic 55.0%
! ATGGGTTACCGGAGGATCTC+CGG - chr1.2:68914015-68914034 MS.gene60557:CDS 55.0%
ATCAACCGCCGGAGATCCTC+CGG + chr1.2:68914026-68914045 None:intergenic 60.0%
GGTTACCGGAGGATCTCCGG+CGG - chr1.2:68914018-68914037 MS.gene60557:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 68914002 68916516 68914002 ID=MS.gene60557
chr1.2 mRNA 68914002 68916516 68914002 ID=MS.gene60557.t1;Parent=MS.gene60557
chr1.2 exon 68916059 68916516 68916059 ID=MS.gene60557.t1.exon1;Parent=MS.gene60557.t1
chr1.2 CDS 68916059 68916516 68916059 ID=cds.MS.gene60557.t1;Parent=MS.gene60557.t1
chr1.2 exon 68914002 68915061 68914002 ID=MS.gene60557.t1.exon2;Parent=MS.gene60557.t1
chr1.2 CDS 68914002 68915061 68914002 ID=cds.MS.gene60557.t1;Parent=MS.gene60557.t1
Gene Sequence

>MS.gene60557

ATGAGTGACCTATATGGGTTACCGGAGGATCTCCGGCGGTTGATAACATCATCAAGAAACACTACACATCTACCAGCTGAACCACCACCTTGTTTGTATGGTGCTGCTGCTACTTTTCCTCCACCAAATTATTCCTATGACCCTATGATAGTTGGTGATGTTTTCTACCCTCGTAGTAGCTTCACTCACCACTATGACTATTCCTCCACTGTTAACCCTACAAATACCACTATAGCTGCTGCTGCTGCAGCAGCTTCTAATTCAGATCATGCTACTGCTTCTACTGCATTCTGTGTAGGTTTAGAAACTGCTGATAAAGGATGGCTTGGTAACTTTGATTCTTGTAACAACAGATGGCCTAGACAAGAGACTCTTTCTCTTCTTGAAATCAGATCTCGTCTTGATTCTAAGTTCAGAGAGAATAATCAGAAAGCACCCTTGTGGAATGAAATTTCTAGGTATATGTTAGTGTCTTAATTAATTACTGGTTTAGCAAGCTTTTTATTTCACTTTTTTTTTTTGGAATTATTTCCTTTTGTGATTGAAAATGACAGGTTTTTTTCCATGATAATTTTCTTTTTTGTCTTGAGTGTTAAAGCTAACTGAAGATCCATGTGTTTTTTTTATTATGATATATTTCTAGTTTTGTCTAAGAATTTTCACTGACTTAGTGTAGTGACTATACTTACTTGCACTGGTTTTTTTCCATGATACTAAATCATTCATTGCAGGTGCAAATTAAGGATTTCTCACACTGAAAGCCTGTCTTTGTTTTGTATATACGTATAGTCCTATTAACCTAATTCGCTCAGCTACCTAGCTAGGGCTATTGGAGTTTTGTTAATTCAAATGCATATATTTGATGTTTCTGACATCATTCATGAAAAGATCAGAATGTTTTCATTATTTTAATCCTTTCAGTACAAAACACTAACATTAATATTCAGCCTATCACAGTATCACTTGATTTGATTTTTTGTTTGAAAATATCATGTGGTCTTGGTCTTCATTATTAGTACAACTTTTTACCCCAAGGTTTCAATTACAATCACATATAATTTTCTAGTATTTCGTATATATCCCACAAATCTTTGCATTGGAGGAGATCAAAGGGTTTTGTAAGAGATTTGTTGACAATTTTAAATTTAAATTGTAGTTACGGTTGTATATTTTTTTATAATATTGTAGAGAATTATAATAAATTTGTTTCATTTTAAACAGCTAAATTTATTTAAACAACTCAAATATGCACAAAGCTGTAATAAATGTGGTTGATATAATATCGATGATTGCATTAGTGTTCTGATATTAATGACATAAAAAATCATTATGTTCAAAAATATTTTTAAAAAAAATTGCAATTAAATTTGTTGATGTGTTTTTTTGCTACAAATTTTGTTGATGTTTTAATGTGTATAACATATAATATAATAGCTTTGTGATCTTCTTGTTTAGGATAATGGCTGAGGAATTTGGGTACCAAAGAAGTGGAAAGAAATGCAAGGAAAAGTTTGAGAATTTGTACAAGTATTACAAGAAGACCAAGGAAGGTAAAGCTAGTAGACAAGATGGTAAGCACTATAGATTTTTCAGACAGCTTGAAGCAATATGTGGAGAACCAAACACAAACTCTCATGCCTCAACTTTAGACAAAATTACCCCTAATGCTTCCTTTGTTGCTACTCAAACTCAAACCTTCACAATTAACCAAGAAAATACTAATGGTGTTGATCACAATTTGATCAATAACCACAAGTATTCACAAAGTCTAAGTTTCTCGAACTCGTCTGAATTTGAGACATCCTCATCTGAAAACAACGATGAGGATCTCTCAACTATTGCACACACTATGAAGTCGTCGAAGGAGAAAGGGCAAAGTGGTCATAGGAGGGCAAGGAAAAGTTGGAGAGGTAAAGTTGAGGAGATTGTAGATTCTCACATGAAAAATATTATTGAGACTCAAGATGCTTGGATGGAGAGAATGTTGAGTGTTGTTGAACAAAGAGAACAAGAGATGGCATCAAAGGAAGAAGAAAGGAAGAGAAAAGAGTCAATGAGGTTTGATCAAGAAATACATGAACTTTGGGCTAAAGAAAAAGCATGGGTTGAAGCAAGAGATTCTGCATTGTTAGAAGTTGTAAGAAAACATATTGGGATACAACTTGAAGCACCAAATAAGAGTAAGAGCCAAGAAGCAAATGATACAACAAATTATGAATACCCTTTTGAAAGTGTGGATAATATTCATAGATGGACAGAAATGGAAATTTCAAACTTGATACAACTAAGGAGTGGTTTTGAACATCAAGTAAGAGAAAAAGGATATTTGGATGATGGAGTTTGGGATGAAATAGGTGAAAGGATGGTTTATATGGGATTTAATAGGAATGGAGCTGAGTGCAAGAAAATATGGGATGATATTAGTATGTCTCTTAGAAGGACAGTGGACTGTGGAGTCAAAAACACAAGGCCTTGGTGTTTGGGACTTAAGCTGACAGATGATGACGACCTTTGA
Protein sequence

>MS.gene60557.t1

MSDLYGLPEDLRRLITSSRNTTHLPAEPPPCLYGAAATFPPPNYSYDPMIVGDVFYPRSSFTHHYDYSSTVNPTNTTIAAAAAAASNSDHATASTAFCVGLETADKGWLGNFDSCNNRWPRQETLSLLEIRSRLDSKFRENNQKAPLWNEISRIMAEEFGYQRSGKKCKEKFENLYKYYKKTKEGKASRQDGKHYRFFRQLEAICGEPNTNSHASTLDKITPNASFVATQTQTFTINQENTNGVDHNLINNHKYSQSLSFSNSSEFETSSSENNDEDLSTIAHTMKSSKEKGQSGHRRARKSWRGKVEEIVDSHMKNIIETQDAWMERMLSVVEQREQEMASKEEERKRKESMRFDQEIHELWAKEKAWVEARDSALLEVVRKHIGIQLEAPNKSKSQEANDTTNYEYPFESVDNIHRWTEMEISNLIQLRSGFEHQVREKGYLDDGVWDEIGERMVYMGFNRNGAECKKIWDDISMSLRRTVDCGVKNTRPWCLGLKLTDDDDL