AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene050206


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050206.t1 MTR_1g492790 99.145 351 3 0 1 351 151 501 0.0 728
MS.gene050206.t1 MTR_1g060990 41.129 248 113 9 1 248 168 382 2.43e-48 170
MS.gene050206.t1 MTR_1g098900 30.827 266 138 9 4 229 96 355 3.89e-26 109
MS.gene050206.t1 MTR_1g098870 25.348 359 205 12 4 322 94 429 4.91e-23 100
MS.gene050206.t1 MTR_6g035370 61.538 52 20 0 4 55 94 145 2.66e-15 77.4
MS.gene050206.t1 MTR_2g026725 27.193 228 116 8 153 338 114 333 2.35e-14 73.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050206.t1 AT5G03680 38.418 354 169 9 1 327 156 487 2.41e-62 209
MS.gene050206.t1 AT3G10000 38.306 248 132 7 1 245 125 354 3.04e-40 148
MS.gene050206.t1 AT3G10000 37.500 240 129 7 9 245 123 344 1.93e-36 137
MS.gene050206.t1 AT1G76890 28.676 408 199 12 4 338 80 468 2.87e-32 127
MS.gene050206.t1 AT1G76880 58.929 56 23 0 4 59 100 155 2.72e-14 74.3

Find 66 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTGGCTCTTACTCTTATT+TGG 0.155961 1.1:+67161633 None:intergenic
TTGTTGTATCATTTGCTTCT+TGG 0.177243 1.1:+67161616 None:intergenic
TGCATTTCTTTCCACTTCTT+TGG 0.203428 1.1:+67162324 None:intergenic
AGGATAATGGCTGAGGAATT+TGG 0.204963 1.1:-67162350 None:intergenic
GAACATCAAGTAAGAGAAAA+AGG 0.239292 1.1:-67161504 MS.gene050206:CDS
AGGTGAAAGGATGGTTTATA+TGG 0.259177 1.1:-67161451 MS.gene050206:CDS
GATATTTGGATGATGGAGTT+TGG 0.262012 1.1:-67161482 MS.gene050206:CDS
AAACACAAGGCCTTGGTGTT+TGG 0.270837 1.1:-67161343 MS.gene050206:CDS
GAGCTGAGTGCAAGAAAATA+TGG 0.284773 1.1:-67161413 MS.gene050206:CDS
AACACAAGGCCTTGGTGTTT+GGG 0.305639 1.1:-67161342 MS.gene050206:CDS
TCTACTAGCTTTACCTTCCT+TGG 0.314459 1.1:+67162262 None:intergenic
TGGTTTATATGGGATTTAAT+AGG 0.331668 1.1:-67161440 MS.gene050206:CDS
ATCAAGAAATACATGAACTT+TGG 0.336932 1.1:-67161740 MS.gene050206:CDS
GGATAATGGCTGAGGAATTT+GGG 0.344397 1.1:-67162349 None:intergenic
ATATTTGGATGATGGAGTTT+GGG 0.376772 1.1:-67161481 MS.gene050206:CDS
GATGGAGTTTGGGATGAAAT+AGG 0.383462 1.1:-67161471 MS.gene050206:CDS
TCAAGAAATACATGAACTTT+GGG 0.384682 1.1:-67161739 MS.gene050206:CDS
GTAGCAACAAAGGAAGCATT+AGG 0.385971 1.1:+67162143 None:intergenic
GAAGTTGTAAGAAAACATAT+TGG 0.406391 1.1:-67161672 MS.gene050206:CDS
AAGTTGTAAGAAAACATATT+GGG 0.418479 1.1:-67161671 MS.gene050206:CDS
TAGCAACAAAGGAAGCATTA+GGG 0.434533 1.1:+67162144 None:intergenic
GAGGCATGAGAGTTTGTGTT+TGG 0.435521 1.1:+67162185 None:intergenic
AGAAAGGGCAAAGTGGTCAT+AGG 0.438060 1.1:-67161938 MS.gene050206:CDS
ATATTAGTATGTCTCTTAGA+AGG 0.438578 1.1:-67161386 MS.gene050206:CDS
TATATGGGATTTAATAGGAA+TGG 0.452119 1.1:-67161435 MS.gene050206:CDS
ATATTATCCACACTTTCAAA+AGG 0.458934 1.1:+67161582 None:intergenic
TATTCATAGATGGACAGAAA+TGG 0.466542 1.1:-67161562 MS.gene050206:CDS
AGAACAAGAGATGGCATCAA+AGG 0.471241 1.1:-67161799 MS.gene050206:CDS
TTTCAAACTTGATACAACTA+AGG 0.475487 1.1:-67161536 MS.gene050206:CDS
ATTAACCAAGAAAATACTAA+TGG 0.483532 1.1:-67162101 MS.gene050206:CDS
TTATTGAGACTCAAGATGCT+TGG 0.485535 1.1:-67161854 MS.gene050206:CDS
ATGAAGTCGTCGAAGGAGAA+AGG 0.490837 1.1:-67161954 MS.gene050206:CDS
GGTGAAAGGATGGTTTATAT+GGG 0.497182 1.1:-67161450 MS.gene050206:CDS
GGATGAAATAGGTGAAAGGA+TGG 0.499715 1.1:-67161460 MS.gene050206:CDS
GTGTGGATAATATTCATAGA+TGG 0.500320 1.1:-67161572 MS.gene050206:CDS
CTTGTTTAGGATAATGGCTG+AGG 0.502416 1.1:-67162357 None:intergenic
ATAGGAGGGCAAGGAAAAGT+TGG 0.504848 1.1:-67161920 MS.gene050206:CDS
TATTATCCACACTTTCAAAA+GGG 0.515340 1.1:+67161583 None:intergenic
TGAGACTCAAGATGCTTGGA+TGG 0.522893 1.1:-67161850 MS.gene050206:CDS
AGACAGCTTGAAGCAATATG+TGG 0.533898 1.1:-67162212 MS.gene050206:CDS
TGAAGTCGTCGAAGGAGAAA+GGG 0.536001 1.1:-67161953 MS.gene050206:CDS
TGGCATCAAAGGAAGAAGAA+AGG 0.537348 1.1:-67161788 MS.gene050206:CDS
TTAGACTTTGTGAATACTTG+TGG 0.538073 1.1:+67162054 None:intergenic
GGAAGAGAAAAGAGTCAATG+AGG 0.550829 1.1:-67161767 MS.gene050206:CDS
TTGAGTTTGAGTAGCAACAA+AGG 0.562015 1.1:+67162133 None:intergenic
AAGGGCAAAGTGGTCATAGG+AGG 0.562963 1.1:-67161935 MS.gene050206:CDS
GGTAAAGCTAGTAGACAAGA+TGG 0.568681 1.1:-67162254 MS.gene050206:CDS
GAATTTGGGTACCAAAGAAG+TGG 0.582190 1.1:-67162335 MS.gene050206:CDS
AGGGCAAGGAAAAGTTGGAG+AGG 0.591096 1.1:-67161915 MS.gene050206:CDS
AGCTGAGTGCAAGAAAATAT+GGG 0.595363 1.1:-67161412 MS.gene050206:CDS
TTTGGGATGAAATAGGTGAA+AGG 0.600563 1.1:-67161464 MS.gene050206:CDS
TATTACAAGAAGACCAAGGA+AGG 0.601931 1.1:-67162275 MS.gene050206:CDS
AAGTTGGAGAGGTAAAGTTG+AGG 0.607935 1.1:-67161904 MS.gene050206:CDS
AAAGTGGTCATAGGAGGGCA+AGG 0.623960 1.1:-67161929 MS.gene050206:CDS
TGAACAAAGAGAACAAGAGA+TGG 0.625612 1.1:-67161808 MS.gene050206:CDS
AGCAACAAAGGAAGCATTAG+GGG 0.629023 1.1:+67162145 None:intergenic
CTTAGAAGGACAGTGGACTG+TGG 0.637596 1.1:-67161372 MS.gene050206:CDS
AAGAAGTGGAAAGAAATGCA+AGG 0.641850 1.1:-67162321 MS.gene050206:CDS
AGGGCAAAGTGGTCATAGGA+GGG 0.643035 1.1:-67161934 MS.gene050206:CDS
ACACACTATGAAGTCGTCGA+AGG 0.660577 1.1:-67161961 MS.gene050206:CDS
TATGTCTCTTAGAAGGACAG+TGG 0.662720 1.1:-67161379 MS.gene050206:CDS
CTCATCTGAAAACAACGATG+AGG 0.668787 1.1:-67162000 MS.gene050206:CDS
AGCTTAAGTCCCAAACACCA+AGG 0.671681 1.1:+67161333 None:intergenic
AACTTGATACAACTAAGGAG+TGG 0.671997 1.1:-67161531 MS.gene050206:CDS
TCGAAGGAGAAAGGGCAAAG+TGG 0.682624 1.1:-67161945 MS.gene050206:CDS
CAAGTATTACAAGAAGACCA+AGG 0.709647 1.1:-67162279 MS.gene050206:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGTTGTAAGAAAACATATT+GGG - chr1.1:67161984-67162003 MS.gene050206:CDS 20.0%
!! ATTAACCAAGAAAATACTAA+TGG - chr1.1:67161554-67161573 MS.gene050206:CDS 20.0%
! AGTAAGAGAAAAAGGATATT+TGG - chr1.1:67162159-67162178 MS.gene050206:CDS 25.0%
! ATATTAGTATGTCTCTTAGA+AGG - chr1.1:67162269-67162288 MS.gene050206:CDS 25.0%
! ATATTATCCACACTTTCAAA+AGG + chr1.1:67162076-67162095 None:intergenic 25.0%
! ATCAAGAAATACATGAACTT+TGG - chr1.1:67161915-67161934 MS.gene050206:CDS 25.0%
! GAAGTTGTAAGAAAACATAT+TGG - chr1.1:67161983-67162002 MS.gene050206:CDS 25.0%
! TATATGGGATTTAATAGGAA+TGG - chr1.1:67162220-67162239 MS.gene050206:CDS 25.0%
! TATTATCCACACTTTCAAAA+GGG + chr1.1:67162075-67162094 None:intergenic 25.0%
! TCAAGAAATACATGAACTTT+GGG - chr1.1:67161916-67161935 MS.gene050206:CDS 25.0%
! TGGTTTATATGGGATTTAAT+AGG - chr1.1:67162215-67162234 MS.gene050206:CDS 25.0%
! TTTCAAACTTGATACAACTA+AGG - chr1.1:67162119-67162138 MS.gene050206:CDS 25.0%
!! ATTTTCTTGGTTAATTGTGA+AGG + chr1.1:67161549-67161568 None:intergenic 25.0%
GAAAAAGGATATTTGGATGA+TGG - chr1.1:67162166-67162185 MS.gene050206:CDS 30.0%
GAACATCAAGTAAGAGAAAA+AGG - chr1.1:67162151-67162170 MS.gene050206:CDS 30.0%
GTGTGGATAATATTCATAGA+TGG - chr1.1:67162083-67162102 MS.gene050206:CDS 30.0%
TATTCATAGATGGACAGAAA+TGG - chr1.1:67162093-67162112 MS.gene050206:CDS 30.0%
TTAGACTTTGTGAATACTTG+TGG + chr1.1:67161604-67161623 None:intergenic 30.0%
TTGTTGTATCATTTGCTTCT+TGG + chr1.1:67162042-67162061 None:intergenic 30.0%
! CAACACCATTAGTATTTTCT+TGG + chr1.1:67161562-67161581 None:intergenic 30.0%
!! ATATTTGGATGATGGAGTTT+GGG - chr1.1:67162174-67162193 MS.gene050206:CDS 30.0%
!! GGTAATTTTGTCTAAAGTTG+AGG + chr1.1:67161492-67161511 None:intergenic 30.0%
AACTTGATACAACTAAGGAG+TGG - chr1.1:67162124-67162143 MS.gene050206:CDS 35.0%
AAGAAGTGGAAAGAAATGCA+AGG - chr1.1:67161334-67161353 MS.gene050206:CDS 35.0%
AGCTGAGTGCAAGAAAATAT+GGG - chr1.1:67162243-67162262 MS.gene050206:CDS 35.0%
AGGTGAAAGGATGGTTTATA+TGG - chr1.1:67162204-67162223 MS.gene050206:CDS 35.0%
CAAGTATTACAAGAAGACCA+AGG - chr1.1:67161376-67161395 MS.gene050206:CDS 35.0%
GGTGAAAGGATGGTTTATAT+GGG - chr1.1:67162205-67162224 MS.gene050206:CDS 35.0%
TATTACAAGAAGACCAAGGA+AGG - chr1.1:67161380-67161399 MS.gene050206:CDS 35.0%
TGAACAAAGAGAACAAGAGA+TGG - chr1.1:67161847-67161866 MS.gene050206:CDS 35.0%
TGCATTTCTTTCCACTTCTT+TGG + chr1.1:67161334-67161353 None:intergenic 35.0%
TTATTGAGACTCAAGATGCT+TGG - chr1.1:67161801-67161820 MS.gene050206:CDS 35.0%
TTGAGTTTGAGTAGCAACAA+AGG + chr1.1:67161525-67161544 None:intergenic 35.0%
TTTGGGCTAAAGAAAAAGCA+TGG - chr1.1:67161933-67161952 MS.gene050206:CDS 35.0%
! TCTTGGCTCTTACTCTTATT+TGG + chr1.1:67162025-67162044 None:intergenic 35.0%
! TTGGGCTAAAGAAAAAGCAT+GGG - chr1.1:67161934-67161953 MS.gene050206:CDS 35.0%
! TTTGGGATGAAATAGGTGAA+AGG - chr1.1:67162191-67162210 MS.gene050206:CDS 35.0%
!! GATATTTGGATGATGGAGTT+TGG - chr1.1:67162173-67162192 MS.gene050206:CDS 35.0%
!! TAGCAACAAAGGAAGCATTA+GGG + chr1.1:67161514-67161533 None:intergenic 35.0%
!! TGAATACCCTTTTGAAAGTG+TGG - chr1.1:67162066-67162085 MS.gene050206:CDS 35.0%
AAGTTGGAGAGGTAAAGTTG+AGG - chr1.1:67161751-67161770 MS.gene050206:CDS 40.0%
ACTGTGGAGTCAAAAACACA+AGG - chr1.1:67162299-67162318 MS.gene050206:CDS 40.0%
AGAACAAGAGATGGCATCAA+AGG - chr1.1:67161856-67161875 MS.gene050206:CDS 40.0%
AGACAGCTTGAAGCAATATG+TGG - chr1.1:67161443-67161462 MS.gene050206:CDS 40.0%
CTCATCTGAAAACAACGATG+AGG - chr1.1:67161655-67161674 MS.gene050206:CDS 40.0%
GAATTTGGGTACCAAAGAAG+TGG - chr1.1:67161320-67161339 MS.gene050206:CDS 40.0%
GAGCTGAGTGCAAGAAAATA+TGG - chr1.1:67162242-67162261 MS.gene050206:CDS 40.0%
GGAAGAGAAAAGAGTCAATG+AGG - chr1.1:67161888-67161907 MS.gene050206:CDS 40.0%
GGATGAAATAGGTGAAAGGA+TGG - chr1.1:67162195-67162214 MS.gene050206:CDS 40.0%
GGTAAAGCTAGTAGACAAGA+TGG - chr1.1:67161401-67161420 MS.gene050206:CDS 40.0%
TATGTCTCTTAGAAGGACAG+TGG - chr1.1:67162276-67162295 MS.gene050206:CDS 40.0%
TGGCATCAAAGGAAGAAGAA+AGG - chr1.1:67161867-67161886 MS.gene050206:CDS 40.0%
! GATGGAGTTTGGGATGAAAT+AGG - chr1.1:67162184-67162203 MS.gene050206:CDS 40.0%
!! AGCAACAAAGGAAGCATTAG+GGG + chr1.1:67161513-67161532 None:intergenic 40.0%
!! CTCATCGTTGTTTTCAGATG+AGG + chr1.1:67161657-67161676 None:intergenic 40.0%
!! GTAGCAACAAAGGAAGCATT+AGG + chr1.1:67161515-67161534 None:intergenic 40.0%
!! TCTACTAGCTTTACCTTCCT+TGG + chr1.1:67161396-67161415 None:intergenic 40.0%
ACACACTATGAAGTCGTCGA+AGG - chr1.1:67161694-67161713 MS.gene050206:CDS 45.0%
AGAAAGGGCAAAGTGGTCAT+AGG - chr1.1:67161717-67161736 MS.gene050206:CDS 45.0%
AGCTTAAGTCCCAAACACCA+AGG + chr1.1:67162325-67162344 None:intergenic 45.0%
ATAGGAGGGCAAGGAAAAGT+TGG - chr1.1:67161735-67161754 MS.gene050206:CDS 45.0%
ATGAAGTCGTCGAAGGAGAA+AGG - chr1.1:67161701-67161720 MS.gene050206:CDS 45.0%
GAGGCATGAGAGTTTGTGTT+TGG + chr1.1:67161473-67161492 None:intergenic 45.0%
GAGTCAAAAACACAAGGCCT+TGG - chr1.1:67162305-67162324 MS.gene050206:CDS 45.0%
TGAAGTCGTCGAAGGAGAAA+GGG - chr1.1:67161702-67161721 MS.gene050206:CDS 45.0%
TGAGACTCAAGATGCTTGGA+TGG - chr1.1:67161805-67161824 MS.gene050206:CDS 45.0%
!! AAACACAAGGCCTTGGTGTT+TGG - chr1.1:67162312-67162331 MS.gene050206:CDS 45.0%
!! AACACAAGGCCTTGGTGTTT+GGG - chr1.1:67162313-67162332 MS.gene050206:CDS 45.0%
AAAGTGGTCATAGGAGGGCA+AGG - chr1.1:67161726-67161745 MS.gene050206:CDS 50.0%
AAGGGCAAAGTGGTCATAGG+AGG - chr1.1:67161720-67161739 MS.gene050206:CDS 50.0%
AGGGCAAAGTGGTCATAGGA+GGG - chr1.1:67161721-67161740 MS.gene050206:CDS 50.0%
AGGGCAAGGAAAAGTTGGAG+AGG - chr1.1:67161740-67161759 MS.gene050206:CDS 50.0%
TCGAAGGAGAAAGGGCAAAG+TGG - chr1.1:67161710-67161729 MS.gene050206:CDS 50.0%
!! CTTAGAAGGACAGTGGACTG+TGG - chr1.1:67162283-67162302 MS.gene050206:CDS 50.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 67161311 67162366 67161311 ID=MS.gene050206
chr1.1 mRNA 67161311 67162366 67161311 ID=MS.gene050206.t1;Parent=MS.gene050206
chr1.1 exon 67161311 67162366 67161311 ID=MS.gene050206.t1.exon1;Parent=MS.gene050206.t1
chr1.1 CDS 67161311 67162366 67161311 ID=cds.MS.gene050206.t1;Parent=MS.gene050206.t1
Gene Sequence

>MS.gene050206

ATGGCTGAGGAATTTGGGTACCAAAGAAGTGGAAAGAAATGCAAGGAAAAGTTTGAGAATTTGTACAAGTATTACAAGAAGACCAAGGAAGGTAAAGCTAGTAGACAAGATGGTAAGCACTATAGATTTTTCAGACAGCTTGAAGCAATATGTGGAGAACCAAACACAAACTCTCATGCCTCAACTTTAGACAAAATTACCCCTAATGCTTCCTTTGTTGCTACTCAAACTCAAACCTTCACAATTAACCAAGAAAATACTAATGGTGTTGATCACAATTTGATCAATAACCACAAGTATTCACAAAGTCTAAGTTTCTCGAACTCGTCTGAATTTGAGACATCCTCATCTGAAAACAACGATGAGGATCTCTCAACTATTGCACACACTATGAAGTCGTCGAAGGAGAAAGGGCAAAGTGGTCATAGGAGGGCAAGGAAAAGTTGGAGAGGTAAAGTTGAGGAGATTGTAGATTCTCACATGAAAAATATTATTGAGACTCAAGATGCTTGGATGGAGAGAATGTTGAGTGTTGTTGAACAAAGAGAACAAGAGATGGCATCAAAGGAAGAAGAAAGGAAGAGAAAAGAGTCAATGAGGTTTGATCAAGAAATACATGAACTTTGGGCTAAAGAAAAAGCATGGGTTGAAGCAAGAGATTCTGCATTGTTAGAAGTTGTAAGAAAACATATTGGGATACAACTTGAAGCACCAAATAAGAGTAAGAGCCAAGAAGCAAATGATACAACAAATTATGAATACCCTTTTGAAAGTGTGGATAATATTCATAGATGGACAGAAATGGAAATTTCAAACTTGATACAACTAAGGAGTGGTTTTGAACATCAAGTAAGAGAAAAAGGATATTTGGATGATGGAGTTTGGGATGAAATAGGTGAAAGGATGGTTTATATGGGATTTAATAGGAATGGAGCTGAGTGCAAGAAAATATGGGATGATATTAGTATGTCTCTTAGAAGGACAGTGGACTGTGGAGTCAAAAACACAAGGCCTTGGTGTTTGGGACTTAAGCTGACAGATGATGACGACCTTTGA

Protein sequence

>MS.gene050206.t1

MAEEFGYQRSGKKCKEKFENLYKYYKKTKEGKASRQDGKHYRFFRQLEAICGEPNTNSHASTLDKITPNASFVATQTQTFTINQENTNGVDHNLINNHKYSQSLSFSNSSEFETSSSENNDEDLSTIAHTMKSSKEKGQSGHRRARKSWRGKVEEIVDSHMKNIIETQDAWMERMLSVVEQREQEMASKEEERKRKESMRFDQEIHELWAKEKAWVEARDSALLEVVRKHIGIQLEAPNKSKSQEANDTTNYEYPFESVDNIHRWTEMEISNLIQLRSGFEHQVREKGYLDDGVWDEIGERMVYMGFNRNGAECKKIWDDISMSLRRTVDCGVKNTRPWCLGLKLTDDDDL