Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60727.t1 | RHN81635.1 | 64.5 | 141 | 41 | 2 | 8 | 142 | 6 | 143 | 2.10E-35 | 158.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60727.t1 | A0A396K0M0 | 64.5 | 141 | 41 | 2 | 8 | 142 | 6 | 143 | 1.5e-35 | 158.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049853 | MS.gene60727 | 0.81074 | 9.92E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 13 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATTTCTGTCACACATTACT+TGG | 0.271603 | 1.2:+71268674 | None:intergenic |
| GTGTGACAGAAATGTTAATT+TGG | 0.350573 | 1.2:-71268665 | MS.gene60727:CDS |
| AGCGTATCAAGATTTAAAAT+TGG | 0.351945 | 1.2:-71268743 | MS.gene60727:CDS |
| TGTATCAATTCTTTGAGTCT+TGG | 0.440490 | 1.2:+71268801 | None:intergenic |
| AACAAGTGCAGAAGCAAGAC+TGG | 0.444644 | 1.2:-71268779 | MS.gene60727:CDS |
| TGAGGAGTTTGTTAAAGCTC+TGG | 0.448360 | 1.2:-71268833 | MS.gene60727:CDS |
| CTCCTTGTCAAAATATATGA+AGG | 0.490071 | 1.2:-71268713 | MS.gene60727:CDS |
| TCTCTAAGAATTGATACAGT+AGG | 0.554329 | 1.2:-71268949 | MS.gene60727:CDS |
| TTTAGACAATTTGGTGAAGG+TGG | 0.561718 | 1.2:-71268866 | MS.gene60727:CDS |
| GGAGTTTGTTAAAGCTCTGG+AGG | 0.615592 | 1.2:-71268830 | MS.gene60727:CDS |
| AGTAGGTGACACAACAACAC+TGG | 0.626223 | 1.2:-71268932 | MS.gene60727:CDS |
| TTGGTGAAGGTGGCGAATTG+CGG | 0.700526 | 1.2:-71268856 | MS.gene60727:CDS |
| GAAGGTGGCGAATTGCGGTG+AGG | 0.752986 | 1.2:-71268851 | MS.gene60727:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATAAAGCATATAAGTAATT+AGG | - | chr1.2:71268913-71268932 | MS.gene60727:CDS | 15.0% |
| !! | AGCGTATCAAGATTTAAAAT+TGG | - | chr1.2:71268760-71268779 | MS.gene60727:CDS | 25.0% |
| !!! | TCAACGTTTTTTAGACATTA+TGG | - | chr1.2:71268871-71268890 | MS.gene60727:CDS | 25.0% |
| !!! | TCAACTTGTTTTAGACAATT+TGG | - | chr1.2:71268628-71268647 | MS.gene60727:CDS | 25.0% |
| !!! | TTCCTTCATATATTTTGACA+AGG | + | chr1.2:71268795-71268814 | None:intergenic | 25.0% |
| CTCCTTGTCAAAATATATGA+AGG | - | chr1.2:71268790-71268809 | MS.gene60727:CDS | 30.0% | |
| GTCTAAAAAACGTTGAAACT+GGG | + | chr1.2:71268867-71268886 | None:intergenic | 30.0% | |
| GTGTGACAGAAATGTTAATT+TGG | - | chr1.2:71268838-71268857 | MS.gene60727:CDS | 30.0% | |
| TGTATCAATTCTTTGAGTCT+TGG | + | chr1.2:71268705-71268724 | None:intergenic | 30.0% | |
| TGTCTAAAAAACGTTGAAAC+TGG | + | chr1.2:71268868-71268887 | None:intergenic | 30.0% | |
| ! | CGTTGGTTAAATTTGTTTAC+AGG | - | chr1.2:71268978-71268997 | MS.gene60727:CDS | 30.0% |
| ! | TCTCTAAGAATTGATACAGT+AGG | - | chr1.2:71268554-71268573 | MS.gene60727:intron | 30.0% |
| !! | TGTTTTAGACAATTTGGTGA+AGG | - | chr1.2:71268634-71268653 | MS.gene60727:CDS | 30.0% |
| CATTTCTGTCACACATTACT+TGG | + | chr1.2:71268832-71268851 | None:intergenic | 35.0% | |
| ! | TTTAGACAATTTGGTGAAGG+TGG | - | chr1.2:71268637-71268656 | MS.gene60727:CDS | 35.0% |
| CTTTGTTCAACGTGATACGT+TGG | - | chr1.2:71268961-71268980 | MS.gene60727:CDS | 40.0% | |
| !! | TGAGGAGTTTGTTAAAGCTC+TGG | - | chr1.2:71268670-71268689 | MS.gene60727:CDS | 40.0% |
| AACAAGTGCAGAAGCAAGAC+TGG | - | chr1.2:71268724-71268743 | MS.gene60727:CDS | 45.0% | |
| AGTAGGTGACACAACAACAC+TGG | - | chr1.2:71268571-71268590 | MS.gene60727:intron | 45.0% | |
| !! | GGAGTTTGTTAAAGCTCTGG+AGG | - | chr1.2:71268673-71268692 | MS.gene60727:CDS | 45.0% |
| TTGGTGAAGGTGGCGAATTG+CGG | - | chr1.2:71268647-71268666 | MS.gene60727:CDS | 50.0% | |
| GAAGGTGGCGAATTGCGGTG+AGG | - | chr1.2:71268652-71268671 | MS.gene60727:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 71268503 | 71269022 | 71268503 | ID=MS.gene60727 |
| chr1.2 | mRNA | 71268503 | 71269022 | 71268503 | ID=MS.gene60727.t1;Parent=MS.gene60727 |
| chr1.2 | exon | 71268599 | 71269022 | 71268599 | ID=MS.gene60727.t1.exon1;Parent=MS.gene60727.t1 |
| chr1.2 | CDS | 71268599 | 71269022 | 71268599 | ID=cds.MS.gene60727.t1;Parent=MS.gene60727.t1 |
| chr1.2 | exon | 71268503 | 71268525 | 71268503 | ID=MS.gene60727.t1.exon2;Parent=MS.gene60727.t1 |
| chr1.2 | CDS | 71268503 | 71268525 | 71268503 | ID=cds.MS.gene60727.t1;Parent=MS.gene60727.t1 |
| Gene Sequence |
| Protein sequence |
