AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene60816

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60816.t1 MTR_1g096030 98.915 461 5 0 1 461 1 461 0.0 943
MS.gene60816.t1 MTR_7g027190 33.898 413 221 14 70 459 290 673 9.54e-64 218
MS.gene60816.t1 MTR_7g074650 32.718 379 222 10 85 459 443 792 3.62e-52 188
MS.gene60816.t1 MTR_4g102790 32.426 404 232 13 74 461 286 664 1.25e-51 185
MS.gene60816.t1 MTR_3g065980 31.463 410 222 15 74 459 166 540 1.94e-50 180
MS.gene60816.t1 MTR_5g097480 30.995 442 250 15 38 459 138 544 1.60e-48 174
MS.gene60816.t1 MTR_3g089055 28.967 397 238 12 79 459 202 570 2.43e-45 166
MS.gene60816.t1 MTR_3g089055 28.967 397 238 12 79 459 197 565 2.53e-45 166
MS.gene60816.t1 MTR_1g029420 29.723 397 235 12 79 459 224 592 3.36e-45 166
MS.gene60816.t1 MTR_5g094450 29.064 406 234 14 77 459 158 532 9.93e-45 164
MS.gene60816.t1 MTR_4g104020 29.782 413 234 15 72 460 141 521 1.17e-44 164
MS.gene60816.t1 MTR_4g077760 32.161 398 225 13 76 459 189 555 2.36e-43 160
MS.gene60816.t1 MTR_3g056110 28.894 398 241 13 77 459 172 542 3.87e-43 160
MS.gene60816.t1 MTR_1g069725 30.576 399 232 15 70 458 99 462 2.48e-42 156
MS.gene60816.t1 MTR_4g133660 30.348 402 233 15 75 458 170 542 3.19e-41 154
MS.gene60816.t1 MTR_5g058860 30.602 415 241 12 70 461 109 499 5.96e-41 153
MS.gene60816.t1 MTR_2g082090 28.817 465 257 17 22 459 162 579 7.51e-41 154
MS.gene60816.t1 MTR_4g064200 30.025 403 237 13 72 458 276 649 1.28e-40 154
MS.gene60816.t1 MTR_3g072710 31.221 426 220 16 70 458 110 499 4.29e-40 150
MS.gene60816.t1 MTR_4g076020 29.381 388 216 11 85 460 89 430 1.15e-39 148
MS.gene60816.t1 MTR_2g026250 30.159 378 210 14 103 459 252 596 1.79e-37 145
MS.gene60816.t1 MTR_8g442410 29.298 413 229 17 74 459 149 525 7.00e-37 142
MS.gene60816.t1 MTR_4g064150 29.750 400 238 14 77 458 358 732 3.40e-36 142
MS.gene60816.t1 MTR_4g064120 29.188 394 236 13 77 452 251 619 4.70e-35 138
MS.gene60816.t1 MTR_4g064180 29.640 361 222 12 77 419 251 597 2.95e-34 135
MS.gene60816.t1 MTR_7g062120 27.204 397 244 14 80 459 372 740 1.06e-33 135
MS.gene60816.t1 MTR_2g097310 29.146 398 231 15 80 458 272 637 1.09e-33 134
MS.gene60816.t1 MTR_2g097467 26.087 414 259 13 61 458 271 653 8.52e-33 132
MS.gene60816.t1 MTR_2g097410 26.895 409 244 14 73 458 364 740 2.43e-32 130
MS.gene60816.t1 MTR_2g097463 26.263 396 250 11 77 458 287 654 2.81e-31 127
MS.gene60816.t1 MTR_2g097473 25.758 396 249 12 77 458 289 653 1.26e-30 125
MS.gene60816.t1 MTR_4g064160 28.173 394 242 15 80 458 316 683 5.86e-30 123
MS.gene60816.t1 MTR_2g034260 25.598 418 260 13 65 459 195 584 1.63e-29 121
MS.gene60816.t1 MTR_2g097390 27.157 394 244 13 80 458 275 640 2.58e-29 121
MS.gene60816.t1 MTR_2g097350 27.411 394 243 15 80 458 274 639 2.20e-28 119
MS.gene60816.t1 MTR_4g097080 25.495 404 246 14 79 459 132 503 6.20e-28 116
MS.gene60816.t1 MTR_2g034250 25.542 415 265 12 65 459 195 585 1.58e-27 115
MS.gene60816.t1 MTR_8g093070 30.479 397 224 18 77 458 136 495 3.40e-27 114
MS.gene60816.t1 MTR_5g015490 27.451 408 239 15 79 459 113 490 7.11e-27 113
MS.gene60816.t1 MTR_2g034280 25.234 428 247 15 65 459 188 575 7.36e-27 114
MS.gene60816.t1 MTR_3g022830 23.661 448 256 13 71 459 16 436 8.47e-27 112
MS.gene60816.t1 MTR_2g097380 30.488 328 177 16 87 396 231 525 1.13e-26 113
MS.gene60816.t1 MTR_7g109580 25.845 414 255 14 67 461 176 556 3.02e-26 112
MS.gene60816.t1 MTR_0092s0100 26.368 402 235 16 77 459 372 731 4.31e-20 94.0
MS.gene60816.t1 MTR_0092s0100 26.368 402 235 16 77 459 369 728 4.37e-20 94.0
MS.gene60816.t1 MTR_6g047750 26.895 409 227 15 81 459 296 662 1.25e-19 92.4
MS.gene60816.t1 MTR_4g076140 28.889 225 150 4 71 293 39 255 4.29e-19 90.1
MS.gene60816.t1 MTR_4g076140 31.325 166 96 4 295 460 323 470 9.39e-18 85.9
MS.gene60816.t1 MTR_7g057230 26.263 396 240 13 81 458 295 656 4.98e-19 90.5
MS.gene60816.t1 MTR_5g009080 27.473 273 160 9 71 336 47 288 6.97e-19 89.4
MS.gene60816.t1 MTR_5g009080 29.070 172 104 4 289 460 326 479 1.61e-18 88.2
MS.gene60816.t1 MTR_5g015950 23.333 450 252 14 76 458 22 445 2.23e-18 87.8
MS.gene60816.t1 MTR_3g053270 23.333 450 252 14 76 458 22 445 2.23e-18 87.8
MS.gene60816.t1 MTR_2g089100 21.480 419 261 12 80 458 52 442 2.35e-18 87.8
MS.gene60816.t1 MTR_2g099110 24.590 366 240 11 111 459 105 451 3.47e-17 84.0
MS.gene60816.t1 MTR_8g020840 22.983 409 257 14 79 459 170 548 1.35e-12 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60816.t1 AT3G54220 34.884 387 204 12 85 458 298 649 5.11e-55 194
MS.gene60816.t1 AT1G14920 32.767 412 225 16 63 459 155 529 1.51e-53 188
MS.gene60816.t1 AT2G04890 31.840 424 252 12 45 459 18 413 2.25e-52 182
MS.gene60816.t1 AT3G03450 32.587 402 222 16 74 459 177 545 2.40e-50 179
MS.gene60816.t1 AT5G41920 32.108 408 229 12 66 460 29 401 3.70e-50 176
MS.gene60816.t1 AT2G01570 30.941 404 223 16 73 459 217 581 2.44e-49 177
MS.gene60816.t1 AT1G21450 30.982 397 231 12 79 459 224 593 3.88e-49 177
MS.gene60816.t1 AT5G17490 32.039 412 224 18 64 459 145 516 1.73e-48 174
MS.gene60816.t1 AT1G66350 31.078 399 221 16 74 459 149 506 4.09e-47 170
MS.gene60816.t1 AT5G48150 30.380 395 232 13 81 459 123 490 1.31e-46 168
MS.gene60816.t1 AT5G48150 30.380 395 232 13 81 459 123 490 1.31e-46 168
MS.gene60816.t1 AT5G48150 30.380 395 232 13 81 459 123 490 1.31e-46 168
MS.gene60816.t1 AT5G48150 30.380 395 232 13 81 459 123 490 1.31e-46 168
MS.gene60816.t1 AT1G55580 31.075 428 221 16 80 459 44 445 3.16e-46 166
MS.gene60816.t1 AT1G63100 29.381 388 227 11 99 460 264 630 5.67e-46 169
MS.gene60816.t1 AT1G63100 29.381 388 227 11 99 460 289 655 1.10e-45 169
MS.gene60816.t1 AT5G66770 32.658 395 229 14 76 459 216 584 9.27e-45 165
MS.gene60816.t1 AT4G08250 31.127 408 238 14 70 460 97 478 2.30e-42 157
MS.gene60816.t1 AT1G50600 29.873 395 235 12 80 459 159 526 1.14e-41 155
MS.gene60816.t1 AT3G50650 33.155 374 214 12 98 459 193 542 1.35e-41 155
MS.gene60816.t1 AT1G50600 29.873 395 235 12 80 459 230 597 5.09e-41 155
MS.gene60816.t1 AT1G50600 29.873 395 235 12 80 459 234 601 6.73e-41 154
MS.gene60816.t1 AT4G17230 29.829 409 227 13 75 459 153 525 6.84e-37 142
MS.gene60816.t1 AT4G17230 29.829 409 227 13 75 459 153 525 6.84e-37 142
MS.gene60816.t1 AT1G50420 27.193 456 235 17 74 459 51 479 1.04e-31 127
MS.gene60816.t1 AT2G29060 27.456 397 250 13 80 461 284 657 1.32e-31 128
MS.gene60816.t1 AT2G29060 27.456 397 250 13 80 461 321 694 1.63e-31 128
MS.gene60816.t1 AT2G37650 25.505 396 252 13 80 460 335 702 2.82e-30 124
MS.gene60816.t1 AT2G37650 25.505 396 252 13 80 460 347 714 3.29e-30 124
MS.gene60816.t1 AT2G29065 25.980 408 241 16 80 458 252 627 2.46e-29 121
MS.gene60816.t1 AT1G07530 26.396 394 250 14 80 458 396 764 4.23e-28 118
MS.gene60816.t1 AT3G46600 26.196 397 251 13 79 460 3 372 1.03e-27 114
MS.gene60816.t1 AT5G59450 25.369 406 259 13 72 459 219 598 3.22e-27 115
MS.gene60816.t1 AT3G46600 26.329 395 251 13 79 459 211 579 3.46e-27 115
MS.gene60816.t1 AT1G07520 25.187 401 252 15 80 458 154 528 4.87e-27 114
MS.gene60816.t1 AT1G07520 25.187 401 252 15 80 458 154 528 4.87e-27 114
MS.gene60816.t1 AT3G46600 26.329 395 251 13 79 459 179 547 5.43e-27 114
MS.gene60816.t1 AT1G07520 25.187 401 252 15 80 458 318 692 6.35e-27 114
MS.gene60816.t1 AT1G07520 25.187 401 252 15 80 458 319 693 7.04e-27 114
MS.gene60816.t1 AT3G60630 29.909 331 172 15 152 459 329 622 8.35e-26 110
MS.gene60816.t1 AT4G37650 23.716 409 258 13 80 459 146 529 1.43e-21 97.8
MS.gene60816.t1 AT5G52510 25.679 405 242 15 81 459 269 640 1.82e-21 97.8
MS.gene60816.t1 AT2G45160 28.997 369 194 18 115 459 315 639 1.97e-21 97.8
MS.gene60816.t1 AT4G00150 27.446 368 208 17 105 459 233 554 1.01e-20 95.5
MS.gene60816.t1 AT4G36710 24.570 407 262 14 66 459 111 485 6.28e-20 92.8
MS.gene60816.t1 AT3G49950 23.058 412 252 13 79 458 29 407 6.60e-19 89.0

Find 56 sgRNAs with CRISPR-Local

Find 76 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAAGGTGGTGGTGGTTTC+AGG 0.088549 1.2:-72384293 MS.gene60816:CDS
GATCAAGAGTAATTAATTTA+AGG 0.159758 1.2:-72384791 MS.gene60816:CDS
TCTCTTTAGGATGGCAAAAT+AGG 0.236883 1.2:-72384242 MS.gene60816:CDS
AGAATCTCTTTCAGATTAAC+TGG 0.292650 1.2:-72384918 MS.gene60816:CDS
TCTTTCAGATTAACTGGTTT+CGG 0.307035 1.2:-72384912 MS.gene60816:CDS
GTTAGTTTCTCAAAAGGTTT+TGG 0.310158 1.2:-72384849 MS.gene60816:CDS
CACAGCAAATCAAGCTATCT+TGG 0.326969 1.2:-72385066 MS.gene60816:CDS
GTGAGAAGTAAGTGAATTAA+TGG 0.332976 1.2:+72385344 None:intergenic
GATGGAAGGGCTATCTCTTT+AGG 0.338311 1.2:-72384255 MS.gene60816:CDS
TCCATCAACATTTCATAGAA+TGG 0.366218 1.2:+72385167 None:intergenic
AGAAACTCTTCGTCGATTGT+TGG 0.372995 1.2:+72385140 None:intergenic
ATGGAGAATCATGGATTTGT+TGG 0.401767 1.2:-72384399 MS.gene60816:CDS
ACCATTCTATGAAATGTTGA+TGG 0.404027 1.2:-72385168 MS.gene60816:CDS
AGAAGGTGGTGGTGGTTTCA+GGG 0.404726 1.2:-72384292 MS.gene60816:CDS
GAGTTTCAAGGTTTGTTAAG+AGG 0.425739 1.2:-72384813 MS.gene60816:CDS
TTCTGTTGATGAAAACAACT+TGG 0.427192 1.2:-72385312 MS.gene60816:CDS
CTGTTTGGTAAAGATCTGTT+AGG 0.427840 1.2:+72385273 None:intergenic
CCACTTCAATTTGAGGAAGA+AGG 0.429497 1.2:-72384309 MS.gene60816:CDS
TTGAAAATAACTGACACATT+GGG 0.429946 1.2:-72384705 MS.gene60816:CDS
CACCGGTGAGGGAAACTGTT+TGG 0.439723 1.2:+72385258 None:intergenic
AAGGTTAAGAATTGAGAAGA+AGG 0.444053 1.2:-72384523 MS.gene60816:CDS
CTTGATAAGAAGGTTCTACT+AGG 0.446731 1.2:+72384621 None:intergenic
TTGCTGTGAAATGAGCAAAT+TGG 0.450939 1.2:+72385081 None:intergenic
AGGTTAGTTAGTTTCTCAAA+AGG 0.468291 1.2:-72384855 MS.gene60816:CDS
AACTCCTATGAAGGCTATGA+AGG 0.469990 1.2:-72385446 MS.gene60816:CDS
TTTGAGGAAGAAGGTGGTGG+TGG 0.477498 1.2:-72384300 MS.gene60816:CDS
AGGGTTTCAGAAAGAGATGA+TGG 0.479941 1.2:-72384273 MS.gene60816:CDS
TTCTTTCTCTATTAACTGAA+AGG 0.482856 1.2:+72385528 None:intergenic
ATTGAAAATAACTGACACAT+TGG 0.484927 1.2:-72384706 MS.gene60816:CDS
TTATTGTCCACTTCAATTTG+AGG 0.488195 1.2:-72384316 MS.gene60816:CDS
CTCAACATTGAAGATTCAAA+TGG 0.488668 1.2:-72385371 MS.gene60816:CDS
TACCAAACAGTTTCCCTCAC+CGG 0.497883 1.2:-72385260 MS.gene60816:CDS
GGAAACATGGAAAAGTAGAA+TGG 0.498264 1.2:-72384418 MS.gene60816:CDS
AGCTTCAAGAAACTGAATCT+AGG 0.507835 1.2:-72384875 MS.gene60816:CDS
AAAAGTAGAATGGAGAATCA+TGG 0.508498 1.2:-72384408 MS.gene60816:CDS
CCTTCTTCCTCAAATTGAAG+TGG 0.513172 1.2:+72384309 None:intergenic
ACTAATAGAAACTCCTATGA+AGG 0.514783 1.2:-72385455 MS.gene60816:CDS
AAATGAGCAAATTGGTAATA+CGG 0.517397 1.2:+72385089 None:intergenic
AACCTTTCAATACTCTCAAG+TGG 0.550308 1.2:+72384540 None:intergenic
GTCTGTGAATCTTGATAAGA+AGG 0.567154 1.2:+72384611 None:intergenic
GACACGTTGAACTGAATCAC+CGG 0.567947 1.2:+72385241 None:intergenic
CAATTTGAGGAAGAAGGTGG+TGG 0.571559 1.2:-72384303 MS.gene60816:CDS
TTGAACTGAATCACCGGTGA+GGG 0.571858 1.2:+72385247 None:intergenic
GTTGTTGTTGTTGAACAAGA+AGG 0.574596 1.2:-72384654 MS.gene60816:CDS
GTCGTCGCATATTTCACCGA+TGG 0.586765 1.2:-72385221 MS.gene60816:CDS
TTTCAGAAAGAGATGATGGA+AGG 0.599854 1.2:-72384269 MS.gene60816:CDS
GCTTAATAACTATGATGATG+TGG 0.611848 1.2:-72384475 MS.gene60816:CDS
AATCAATAACATGAATAGAA+CGG 0.614448 1.2:+72385012 None:intergenic
TACCACTTGAGAGTATTGAA+AGG 0.618047 1.2:-72384542 MS.gene60816:CDS
GAAAGTGATTGAATCAGTGA+AGG 0.619627 1.2:+72384963 None:intergenic
TTCAGAAAGAGATGATGGAA+GGG 0.626052 1.2:-72384268 MS.gene60816:CDS
CTTCAATTTGAGGAAGAAGG+TGG 0.626263 1.2:-72384306 MS.gene60816:CDS
GAAGGGCTATCTCTTTAGGA+TGG 0.630794 1.2:-72384251 MS.gene60816:CDS
AATAACTATGATGATGTGGA+TGG 0.639203 1.2:-72384471 MS.gene60816:CDS
AACTATGATGATGTGGATGG+TGG 0.649454 1.2:-72384468 MS.gene60816:CDS
GTTGAACTGAATCACCGGTG+AGG 0.713037 1.2:+72385246 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAGAGAAAGAAAAAAGAA+GGG - chr1.2:72384257-72384276 MS.gene60816:CDS 20.0%
!! AATATGAAAAAATGGAAACA+TGG - chr1.2:72385344-72385363 MS.gene60816:CDS 20.0%
!! AATCAATAACATGAATAGAA+CGG + chr1.2:72384766-72384785 None:intergenic 20.0%
!! GATCAAGAGTAATTAATTTA+AGG - chr1.2:72384984-72385003 MS.gene60816:CDS 20.0%
!! TAATAGAGAAAGAAAAAAGA+AGG - chr1.2:72384256-72384275 MS.gene60816:CDS 20.0%
!! TTGTGCAAAATATGAAAAAA+TGG - chr1.2:72385336-72385355 MS.gene60816:CDS 20.0%
!!! ACAACTCATTTTGTTTAAAT+AGG + chr1.2:72385047-72385066 None:intergenic 20.0%
!!! ATTGATTTTGATGTTTCATA+TGG - chr1.2:72384779-72384798 MS.gene60816:CDS 20.0%
! ATTGAAAATAACTGACACAT+TGG - chr1.2:72385069-72385088 MS.gene60816:CDS 25.0%
! TTCTTTCTCTATTAACTGAA+AGG + chr1.2:72384250-72384269 None:intergenic 25.0%
! TTGAAAATAACTGACACATT+GGG - chr1.2:72385070-72385089 MS.gene60816:CDS 25.0%
!! AAATGAGCAAATTGGTAATA+CGG + chr1.2:72384689-72384708 None:intergenic 25.0%
!! ATGTTTCATATGGTTTTCAA+TGG - chr1.2:72384789-72384808 MS.gene60816:CDS 25.0%
!!! AACATTGTTTTTGAGTTTCA+AGG - chr1.2:72384950-72384969 MS.gene60816:CDS 25.0%
!!! TTTCTCTTCTTCTTTTTCAA+AGG + chr1.2:72384738-72384757 None:intergenic 25.0%
AAAAGTAGAATGGAGAATCA+TGG - chr1.2:72385367-72385386 MS.gene60816:CDS 30.0%
AAGGTTAAGAATTGAGAAGA+AGG - chr1.2:72385252-72385271 MS.gene60816:CDS 30.0%
ACCATTCTATGAAATGTTGA+TGG - chr1.2:72384607-72384626 MS.gene60816:CDS 30.0%
ACTAATAGAAACTCCTATGA+AGG - chr1.2:72384320-72384339 MS.gene60816:CDS 30.0%
AGAATCTCTTTCAGATTAAC+TGG - chr1.2:72384857-72384876 MS.gene60816:CDS 30.0%
CTCAACATTGAAGATTCAAA+TGG - chr1.2:72384404-72384423 MS.gene60816:CDS 30.0%
GAAGAAGAAAAATGAAACTG+TGG - chr1.2:72385006-72385025 MS.gene60816:CDS 30.0%
GTGAGAAGTAAGTGAATTAA+TGG + chr1.2:72384434-72384453 None:intergenic 30.0%
TCCATCAACATTTCATAGAA+TGG + chr1.2:72384611-72384630 None:intergenic 30.0%
TTATTGTCCACTTCAATTTG+AGG - chr1.2:72385459-72385478 MS.gene60816:CDS 30.0%
TTGTTCAACAACAACAACAA+TGG + chr1.2:72385119-72385138 None:intergenic 30.0%
! AATAACTATGATGATGTGGA+TGG - chr1.2:72385304-72385323 MS.gene60816:CDS 30.0%
! AGAATTGAGAAGAAGGTTTT+TGG - chr1.2:72385259-72385278 MS.gene60816:CDS 30.0%
! AGGTTAGTTAGTTTCTCAAA+AGG - chr1.2:72384920-72384939 MS.gene60816:CDS 30.0%
! GCTTAATAACTATGATGATG+TGG - chr1.2:72385300-72385319 MS.gene60816:CDS 30.0%
! TCTTTCAGATTAACTGGTTT+CGG - chr1.2:72384863-72384882 MS.gene60816:CDS 30.0%
! TTCTGTTGATGAAAACAACT+TGG - chr1.2:72384463-72384482 MS.gene60816:CDS 30.0%
!! GTTAGTTTCTCAAAAGGTTT+TGG - chr1.2:72384926-72384945 MS.gene60816:CDS 30.0%
!!! AGAATGGTGATTTTTTTGTG+AGG + chr1.2:72384595-72384614 None:intergenic 30.0%
AACCTTTCAATACTCTCAAG+TGG + chr1.2:72385238-72385257 None:intergenic 35.0%
AGCTTCAAGAAACTGAATCT+AGG - chr1.2:72384900-72384919 MS.gene60816:CDS 35.0%
ATGGAGAATCATGGATTTGT+TGG - chr1.2:72385376-72385395 MS.gene60816:CDS 35.0%
CAACAACAACAACAATGGAA+GGG + chr1.2:72385114-72385133 None:intergenic 35.0%
CTGTTTGGTAAAGATCTGTT+AGG + chr1.2:72384505-72384524 None:intergenic 35.0%
CTTGATAAGAAGGTTCTACT+AGG + chr1.2:72385157-72385176 None:intergenic 35.0%
CTTTCAGAAAAAGCAACAAG+TGG - chr1.2:72384830-72384849 MS.gene60816:CDS 35.0%
GAAAGTGATTGAATCAGTGA+AGG + chr1.2:72384815-72384834 None:intergenic 35.0%
GAAGAAAAATGAAACTGTGG+CGG - chr1.2:72385009-72385028 MS.gene60816:CDS 35.0%
GGAAACATGGAAAAGTAGAA+TGG - chr1.2:72385357-72385376 MS.gene60816:CDS 35.0%
GTCTGTGAATCTTGATAAGA+AGG + chr1.2:72385167-72385186 None:intergenic 35.0%
TCAACAACAACAACAATGGA+AGG + chr1.2:72385115-72385134 None:intergenic 35.0%
TTGCTGTGAAATGAGCAAAT+TGG + chr1.2:72384697-72384716 None:intergenic 35.0%
! GAGTTTTGCAGTTAAACCAT+CGG + chr1.2:72384573-72384592 None:intergenic 35.0%
! GTTGTTGTTGTTGAACAAGA+AGG - chr1.2:72385121-72385140 MS.gene60816:CDS 35.0%
! TTTTCCTTCATAGCCTTCAT+AGG + chr1.2:72384336-72384355 None:intergenic 35.0%
!! GAGTTTCAAGGTTTGTTAAG+AGG - chr1.2:72384962-72384981 MS.gene60816:CDS 35.0%
!! TACCACTTGAGAGTATTGAA+AGG - chr1.2:72385233-72385252 MS.gene60816:CDS 35.0%
!! TCTCTTTAGGATGGCAAAAT+AGG - chr1.2:72385533-72385552 MS.gene60816:CDS 35.0%
!! TTCAGAAAGAGATGATGGAA+GGG - chr1.2:72385507-72385526 MS.gene60816:CDS 35.0%
!! TTTCAGAAAGAGATGATGGA+AGG - chr1.2:72385506-72385525 MS.gene60816:CDS 35.0%
AACTCCTATGAAGGCTATGA+AGG - chr1.2:72384329-72384348 MS.gene60816:CDS 40.0%
AGGGTTTCAGAAAGAGATGA+TGG - chr1.2:72385502-72385521 MS.gene60816:CDS 40.0%
CACAGCAAATCAAGCTATCT+TGG - chr1.2:72384709-72384728 MS.gene60816:CDS 40.0%
CCACTTCAATTTGAGGAAGA+AGG - chr1.2:72385466-72385485 MS.gene60816:CDS 40.0%
CCTTCTTCCTCAAATTGAAG+TGG + chr1.2:72385469-72385488 None:intergenic 40.0%
CTTCAATTTGAGGAAGAAGG+TGG - chr1.2:72385469-72385488 MS.gene60816:CDS 40.0%
! AACTATGATGATGTGGATGG+TGG - chr1.2:72385307-72385326 MS.gene60816:CDS 40.0%
! AGAAACTCTTCGTCGATTGT+TGG + chr1.2:72384638-72384657 None:intergenic 40.0%
! TTTTGCTTACTGTGTCAGCA+TGG - chr1.2:72385557-72385576 MS.gene60816:CDS 40.0%
CAATTTGAGGAAGAAGGTGG+TGG - chr1.2:72385472-72385491 MS.gene60816:CDS 45.0%
GACACGTTGAACTGAATCAC+CGG + chr1.2:72384537-72384556 None:intergenic 45.0%
GATGGAAGGGCTATCTCTTT+AGG - chr1.2:72385520-72385539 MS.gene60816:CDS 45.0%
TACCAAACAGTTTCCCTCAC+CGG - chr1.2:72384515-72384534 MS.gene60816:CDS 45.0%
TTGAACTGAATCACCGGTGA+GGG + chr1.2:72384531-72384550 None:intergenic 45.0%
! GAAGGGCTATCTCTTTAGGA+TGG - chr1.2:72385524-72385543 MS.gene60816:CDS 45.0%
GTCGTCGCATATTTCACCGA+TGG - chr1.2:72384554-72384573 MS.gene60816:CDS 50.0%
GTTGAACTGAATCACCGGTG+AGG + chr1.2:72384532-72384551 None:intergenic 50.0%
! TTTGAGGAAGAAGGTGGTGG+TGG - chr1.2:72385475-72385494 MS.gene60816:CDS 50.0%
!! AAGAAGGTGGTGGTGGTTTC+AGG - chr1.2:72385482-72385501 MS.gene60816:CDS 50.0%
!! AGAAGGTGGTGGTGGTTTCA+GGG - chr1.2:72385483-72385502 MS.gene60816:CDS 50.0%
CACCGGTGAGGGAAACTGTT+TGG + chr1.2:72384520-72384539 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 72384206 72385591 72384206 ID=MS.gene60816
chr1.2 mRNA 72384206 72385591 72384206 ID=MS.gene60816.t1;Parent=MS.gene60816
chr1.2 exon 72384206 72385591 72384206 ID=MS.gene60816.t1.exon1;Parent=MS.gene60816.t1
chr1.2 CDS 72384206 72385591 72384206 ID=cds.MS.gene60816.t1;Parent=MS.gene60816.t1
Gene Sequence

>MS.gene60816

ATGGAAGCTGATTCAGAAGAAGATGAGCTTCTGAATCTTAGCCTTTCAGTTAATAGAGAAAGAAAAAAGAAGGGAAAAATCATCATAAGTAGAGAAAATAACAACATGATGAGTACTAATAGAAACTCCTATGAAGGCTATGAAGGAAAAATCTTTCATCTTCTTCAAATGAGAGAACAAATGCTAAGAAAAAGCACTCTCAACATTGAAGATTCAAATGGTCTTCCATTAATTCACTTACTTCTCACAACAGCAACTTCTGTTGATGAAAACAACTTGGATTCATCTCTTGAAAACCTAACAGATCTTTACCAAACAGTTTCCCTCACCGGTGATTCAGTTCAACGTGTCGTCGCATATTTCACCGATGGTTTAACTGCAAAACTCCTCACAAAAAAATCACCATTCTATGAAATGTTGATGGAAGAACCAACAATCGACGAAGAGTTTCTCGCATTCACAGATCTCTACAGAGTTTCACCGTATTACCAATTTGCTCATTTCACAGCAAATCAAGCTATCTTGGAAGCCTTTGAAAAAGAAGAAGAGAAAAATAACCGTTCTATTCATGTTATTGATTTTGATGTTTCATATGGTTTTCAATGGCCTTCACTGATTCAATCACTTTCAGAAAAAGCAACAAGTGGCAACAGAATCTCTTTCAGATTAACTGGTTTCGGTAAGAATTTAAAAGAGCTTCAAGAAACTGAATCTAGGTTAGTTAGTTTCTCAAAAGGTTTTGGTAACATTGTTTTTGAGTTTCAAGGTTTGTTAAGAGGATCAAGAGTAATTAATTTAAGGAAGAAGAAAAATGAAACTGTGGCGGTTAATTTAGTTTCCTATTTAAACAAAATGAGTTGTTTATTGAAAATAACTGACACATTGGGATTTGTTCATTCTCTTAACCCTTCCATTGTTGTTGTTGTTGAACAAGAAGGTAGTAAAAATCCTAGTAGAACCTTCTTATCAAGATTCACAGACACATTGCATTATTTTGCAGCTATGTTTGATTCACTTGATGATTGTTTACCACTTGAGAGTATTGAAAGGTTAAGAATTGAGAAGAAGGTTTTTGGTAAAGAGATTAAAAGCATGCTTAATAACTATGATGATGTGGATGGTGGTGTTGATTGTGCAAAATATGAAAAAATGGAAACATGGAAAAGTAGAATGGAGAATCATGGATTTGTTGGTATGAAAATGAGTTCAAAATGTTTAATACAAGCAAAATTGTTGCTTAAGATGAGAACACATTATTGTCCACTTCAATTTGAGGAAGAAGGTGGTGGTGGTTTCAGGGTTTCAGAAAGAGATGATGGAAGGGCTATCTCTTTAGGATGGCAAAATAGGTTTTTGCTTACTGTGTCAGCATGGCAATCACTTTGA
Protein sequence

>MS.gene60816.t1

MEADSEEDELLNLSLSVNRERKKKGKIIISRENNNMMSTNRNSYEGYEGKIFHLLQMREQMLRKSTLNIEDSNGLPLIHLLLTTATSVDENNLDSSLENLTDLYQTVSLTGDSVQRVVAYFTDGLTAKLLTKKSPFYEMLMEEPTIDEEFLAFTDLYRVSPYYQFAHFTANQAILEAFEKEEEKNNRSIHVIDFDVSYGFQWPSLIQSLSEKATSGNRISFRLTGFGKNLKELQETESRLVSFSKGFGNIVFEFQGLLRGSRVINLRKKKNETVAVNLVSYLNKMSCLLKITDTLGFVHSLNPSIVVVVEQEGSKNPSRTFLSRFTDTLHYFAAMFDSLDDCLPLESIERLRIEKKVFGKEIKSMLNNYDDVDGGVDCAKYEKMETWKSRMENHGFVGMKMSSKCLIQAKLLLKMRTHYCPLQFEEEGGGGFRVSERDDGRAISLGWQNRFLLTVSAWQSL