Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051199.t1 | XP_024631981.1 | 91.1 | 292 | 22 | 3 | 1 | 288 | 29 | 320 | 2.50E-153 | 551.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051199.t1 | P36908 | 45.8 | 271 | 136 | 5 | 1 | 267 | 26 | 289 | 4.4e-61 | 236.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051199.t1 | A0A396JAW1 | 91.1 | 292 | 22 | 3 | 1 | 288 | 29 | 320 | 1.8e-153 | 551.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050020 | MS.gene051199 | 0.80957 | 1.77E-50 | -1.69E-46 |
| MS.gene051199 | MS.gene05157 | 0.809163 | 2.17E-50 | -1.69E-46 |
| MS.gene051199 | MS.gene051719 | 0.821479 | 3.95E-53 | -1.69E-46 |
| MS.gene051199 | MS.gene051720 | 0.826431 | 2.72E-54 | -1.69E-46 |
| MS.gene051199 | MS.gene054801 | 0.801854 | 7.40E-49 | -1.69E-46 |
| MS.gene051199 | MS.gene061872 | 0.80524 | 1.47E-49 | -1.69E-46 |
| MS.gene051199 | MS.gene061947 | 0.825776 | 3.90E-54 | -1.69E-46 |
| MS.gene051199 | MS.gene062821 | 0.800814 | 1.21E-48 | -1.69E-46 |
| MS.gene051199 | MS.gene065667 | 0.837182 | 6.04E-57 | -1.69E-46 |
| MS.gene051199 | MS.gene067101 | 0.830513 | 2.82E-55 | -1.69E-46 |
| MS.gene051199 | MS.gene067102 | 0.813663 | 2.28E-51 | -1.69E-46 |
| MS.gene051199 | MS.gene071061 | 0.808626 | 2.82E-50 | -1.69E-46 |
| MS.gene051199 | MS.gene074520 | 0.836863 | 7.29E-57 | -1.69E-46 |
| MS.gene051199 | MS.gene08581 | 0.815052 | 1.13E-51 | -1.69E-46 |
| MS.gene051199 | MS.gene09176 | 0.809233 | 2.09E-50 | -1.69E-46 |
| MS.gene051199 | MS.gene21960 | 0.804202 | 2.42E-49 | -1.69E-46 |
| MS.gene051199 | MS.gene22099 | 0.822531 | 2.25E-53 | -1.69E-46 |
| MS.gene051199 | MS.gene24736 | 0.809138 | 2.19E-50 | -1.69E-46 |
| MS.gene051199 | MS.gene30882 | 0.80477 | 1.84E-49 | -1.69E-46 |
| MS.gene051199 | MS.gene30946 | 0.816844 | 4.48E-52 | -1.69E-46 |
| MS.gene051199 | MS.gene31321 | 0.803424 | 3.51E-49 | -1.69E-46 |
| MS.gene051199 | MS.gene33123 | 0.802064 | 6.70E-49 | -1.69E-46 |
| MS.gene051199 | MS.gene37745 | 0.802658 | 5.05E-49 | -1.69E-46 |
| MS.gene051199 | MS.gene39944 | 0.815102 | 1.10E-51 | -1.69E-46 |
| MS.gene051199 | MS.gene40432 | 0.801634 | 8.21E-49 | -1.69E-46 |
| MS.gene051199 | MS.gene49359 | 0.80027 | 1.56E-48 | -1.69E-46 |
| MS.gene051199 | MS.gene49378 | 0.82652 | 2.59E-54 | -1.69E-46 |
| MS.gene051199 | MS.gene51353 | 0.80634 | 8.63E-50 | -1.69E-46 |
| MS.gene051199 | MS.gene51589 | 0.80095 | 1.13E-48 | -1.69E-46 |
| MS.gene051199 | MS.gene53244 | 0.811182 | 7.96E-51 | -1.69E-46 |
| MS.gene051199 | MS.gene53586 | 0.80889 | 2.48E-50 | -1.69E-46 |
| MS.gene051199 | MS.gene55218 | 0.852914 | 3.38E-61 | -1.69E-46 |
| MS.gene051199 | MS.gene57116 | 0.800332 | 1.51E-48 | -1.69E-46 |
| MS.gene051199 | MS.gene59848 | 0.801304 | 9.59E-49 | -1.69E-46 |
| MS.gene051199 | MS.gene60245 | 0.816389 | 5.67E-52 | -1.69E-46 |
| MS.gene051199 | MS.gene60816 | 0.81064 | 1.04E-50 | -1.69E-46 |
| MS.gene051199 | MS.gene61665 | 0.8222 | 2.69E-53 | -1.69E-46 |
| MS.gene051199 | MS.gene62049 | 0.813444 | 2.55E-51 | -1.69E-46 |
| MS.gene051199 | MS.gene64249 | 0.818966 | 1.49E-52 | -1.69E-46 |
| MS.gene051199 | MS.gene68021 | 0.808643 | 2.80E-50 | -1.69E-46 |
| MS.gene051199 | MS.gene68456 | 0.801841 | 7.44E-49 | -1.69E-46 |
| MS.gene051199 | MS.gene71151 | 0.812261 | 4.63E-51 | -1.69E-46 |
| MS.gene051199 | MS.gene71704 | 0.830324 | 3.13E-55 | -1.69E-46 |
| MS.gene051199 | MS.gene72268 | 0.816719 | 4.78E-52 | -1.69E-46 |
| MS.gene051199 | MS.gene76032 | 0.816907 | 4.34E-52 | -1.69E-46 |
| MS.gene051199 | MS.gene81576 | 0.810298 | 1.24E-50 | -1.69E-46 |
| MS.gene051199 | MS.gene81735 | 0.810486 | 1.13E-50 | -1.69E-46 |
| MS.gene051199 | MS.gene84426 | 0.824115 | 9.61E-54 | -1.69E-46 |
| MS.gene051199 | MS.gene90152 | 0.823172 | 1.60E-53 | -1.69E-46 |
| MS.gene051199 | MS.gene92551 | 0.800721 | 1.26E-48 | -1.69E-46 |
| MS.gene051199 | MS.gene92982 | 0.819785 | 9.68E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051199.t1 | MTR_2g040830 | 63.066 | 287 | 105 | 1 | 1 | 286 | 29 | 315 | 4.42e-129 | 372 |
| MS.gene051199.t1 | MTR_2g040690 | 63.763 | 287 | 103 | 1 | 1 | 286 | 31 | 317 | 6.29e-129 | 372 |
| MS.gene051199.t1 | MTR_2g040740 | 59.582 | 287 | 114 | 2 | 1 | 286 | 29 | 314 | 1.23e-117 | 343 |
| MS.gene051199.t1 | MTR_2g040650 | 57.986 | 288 | 114 | 4 | 1 | 286 | 29 | 311 | 8.62e-107 | 315 |
| MS.gene051199.t1 | MTR_2g040890 | 93.836 | 146 | 6 | 2 | 1 | 143 | 29 | 174 | 1.53e-96 | 284 |
| MS.gene051199.t1 | MTR_2g040900 | 88.028 | 142 | 16 | 1 | 148 | 288 | 17 | 158 | 9.55e-88 | 261 |
| MS.gene051199.t1 | MTR_1g099310 | 45.455 | 275 | 140 | 5 | 1 | 271 | 28 | 296 | 2.19e-77 | 239 |
| MS.gene051199.t1 | MTR_1g099320 | 46.324 | 272 | 136 | 6 | 1 | 267 | 27 | 293 | 2.02e-75 | 234 |
| MS.gene051199.t1 | MTR_0027s0260 | 43.682 | 277 | 142 | 8 | 1 | 271 | 30 | 298 | 6.38e-71 | 223 |
| MS.gene051199.t1 | MTR_1g099290 | 47.212 | 269 | 133 | 6 | 3 | 267 | 31 | 294 | 2.95e-70 | 229 |
| MS.gene051199.t1 | MTR_5g043550 | 44.043 | 277 | 140 | 8 | 1 | 271 | 27 | 294 | 4.75e-70 | 220 |
| MS.gene051199.t1 | MTR_8g055940 | 42.960 | 277 | 143 | 8 | 1 | 271 | 30 | 297 | 1.27e-68 | 217 |
| MS.gene051199.t1 | MTR_7g116850 | 43.165 | 278 | 147 | 7 | 1 | 271 | 53 | 326 | 1.34e-68 | 218 |
| MS.gene051199.t1 | MTR_1g099350 | 41.091 | 275 | 150 | 7 | 1 | 271 | 25 | 291 | 1.12e-65 | 209 |
| MS.gene051199.t1 | MTR_8g467650 | 41.516 | 277 | 150 | 7 | 1 | 271 | 28 | 298 | 2.09e-60 | 196 |
| MS.gene051199.t1 | MTR_7g116350 | 41.852 | 270 | 133 | 7 | 3 | 267 | 30 | 280 | 6.25e-60 | 206 |
| MS.gene051199.t1 | MTR_6g023340 | 40.860 | 279 | 152 | 10 | 1 | 271 | 29 | 302 | 1.27e-59 | 194 |
| MS.gene051199.t1 | MTR_2g040870 | 61.806 | 144 | 54 | 1 | 1 | 143 | 26 | 169 | 2.10e-57 | 184 |
| MS.gene051199.t1 | MTR_2g102020 | 40.357 | 280 | 149 | 8 | 1 | 267 | 35 | 309 | 2.80e-57 | 189 |
| MS.gene051199.t1 | MTR_2g040880 | 59.091 | 132 | 54 | 0 | 155 | 286 | 1 | 132 | 1.48e-47 | 157 |
| MS.gene051199.t1 | MTR_2g040850 | 57.724 | 123 | 43 | 2 | 1 | 115 | 29 | 150 | 4.89e-41 | 144 |
| MS.gene051199.t1 | MTR_2g040850 | 48.718 | 78 | 37 | 1 | 211 | 285 | 158 | 235 | 2.41e-16 | 77.8 |
| MS.gene051199.t1 | MTR_4g077310 | 39.024 | 82 | 46 | 1 | 176 | 257 | 3 | 80 | 3.37e-12 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051199.t1 | AT5G24090 | 42.182 | 275 | 151 | 6 | 1 | 271 | 32 | 302 | 3.09e-68 | 216 |
Find 50 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTATTTACTAACTTCCTTAA+TGG | 0.218965 | 2.3:-48924914 | MS.gene051199:CDS |
| AACTTCCTTAATGGTCAATT+TGG | 0.229685 | 2.3:-48924905 | MS.gene051199:CDS |
| TGTTGACAATGAATTATTTC+GGG | 0.245856 | 2.3:+48925028 | None:intergenic |
| TACTTTGAATGGAATTAGTT+TGG | 0.267005 | 2.3:-48924867 | MS.gene051199:CDS |
| TTGTTGACAATGAATTATTT+CGG | 0.275674 | 2.3:+48925027 | None:intergenic |
| TGGCAATATGGTTGTCTATT+GGG | 0.292929 | 2.3:-48925209 | None:intergenic |
| CTTGGAAGTGTTACTTTGAA+TGG | 0.306397 | 2.3:-48924878 | MS.gene051199:CDS |
| CTATGGAGGAATTGTGATTT+GGG | 0.311164 | 2.3:-48916222 | MS.gene051199:CDS |
| ACTATGGAGGAATTGTGATT+TGG | 0.332298 | 2.3:-48916223 | MS.gene051199:CDS |
| ATAATTCATTGTCAACAAAA+AGG | 0.352905 | 2.3:-48925022 | MS.gene051199:CDS |
| TTGGACGTGGTTAGTTCAAC+TGG | 0.354241 | 2.3:-48916360 | MS.gene051199:CDS |
| TTGCACGATCAAGGAATTCT+TGG | 0.356971 | 2.3:-48916379 | MS.gene051199:CDS |
| GACCTATGAAGTTTAACTTT+GGG | 0.360131 | 2.3:+48925086 | None:intergenic |
| TCTACATACGACAAAATTAC+AGG | 0.369572 | 2.3:-48924796 | MS.gene051199:intron |
| GTGGCAATATGGTTGTCTAT+TGG | 0.373642 | 2.3:-48925210 | None:intergenic |
| CTTCACATAACCTAGTTCAT+TGG | 0.378795 | 2.3:+48908980 | None:intergenic |
| CTTGACAAAGCTATCAAAAC+TGG | 0.381379 | 2.3:-48916482 | MS.gene051199:CDS |
| GTGGACCAAATTGACCATTA+AGG | 0.383641 | 2.3:+48924900 | None:intergenic |
| TGACCTATGAAGTTTAACTT+TGG | 0.403106 | 2.3:+48925085 | None:intergenic |
| TTCCTTCTTGAAAGATATTG+AGG | 0.411809 | 2.3:+48925113 | None:intergenic |
| GGAATTAGTTTGGATATTCA+AGG | 0.439079 | 2.3:-48924857 | MS.gene051199:CDS |
| TTCAAGGAGGTCCAGATCAA+TGG | 0.449888 | 2.3:-48924841 | MS.gene051199:CDS |
| GGCAATATGGTTGTCTATTG+GGG | 0.450971 | 2.3:-48925208 | None:intergenic |
| GCAAAGAACTCCCATTGATC+TGG | 0.465987 | 2.3:+48924830 | None:intergenic |
| GCTTTGTCAAGGTACTGATC+TGG | 0.481492 | 2.3:+48916494 | None:intergenic |
| ATACATTGCTTGCACGATCA+AGG | 0.483316 | 2.3:-48916388 | MS.gene051199:CDS |
| TCAAGGAGGTCCAGATCAAT+GGG | 0.485953 | 2.3:-48924840 | MS.gene051199:CDS |
| GACTATGAAACCTACTCTCT+TGG | 0.486009 | 2.3:-48924971 | MS.gene051199:CDS |
| CTGATCTGGAATAATACATT+GGG | 0.492176 | 2.3:+48916508 | None:intergenic |
| TTGAAATCAACTTGTGACAC+TGG | 0.504021 | 2.3:-48925163 | MS.gene051199:CDS |
| TGTGGTATGTAACCACTACT+AGG | 0.505291 | 2.3:+48916293 | None:intergenic |
| TTCCTCAATATCTTTCAAGA+AGG | 0.510674 | 2.3:-48925115 | MS.gene051199:CDS |
| AATGGTCAATTTGGTCCACT+TGG | 0.524588 | 2.3:-48924896 | MS.gene051199:CDS |
| ACTGATCTGGAATAATACAT+TGG | 0.537010 | 2.3:+48916507 | None:intergenic |
| ATCCCAAAGTTAAACTTCAT+AGG | 0.538270 | 2.3:-48925088 | MS.gene051199:CDS |
| TCGTGCAAGCAATGTATCAT+TGG | 0.540494 | 2.3:+48916394 | None:intergenic |
| TTTCAATCTTATAACTATGG+AGG | 0.547146 | 2.3:-48916236 | MS.gene051199:CDS |
| GGTTTCATAGTCACCTCCAA+TGG | 0.548013 | 2.3:+48924982 | None:intergenic |
| ACGACTTATTCATAAAATGA+AGG | 0.552787 | 2.3:-48909475 | MS.gene051199:CDS |
| TTCTATTGAACCAAGAGAGT+AGG | 0.571812 | 2.3:+48924961 | None:intergenic |
| TGCATCAAGGGATGCACAAG+TGG | 0.572832 | 2.3:+48908956 | None:intergenic |
| TAGGGAACAACCAATGAACT+AGG | 0.582521 | 2.3:-48908990 | MS.gene051199:CDS |
| AACAAAGACAACAAACATTG+AGG | 0.604929 | 2.3:-48909538 | MS.gene051199:intron |
| GCTACTGCAGCTCCTAGTAG+TGG | 0.609914 | 2.3:-48916305 | MS.gene051199:CDS |
| GATCAAGGAATTCTTGGACG+TGG | 0.637226 | 2.3:-48916373 | MS.gene051199:CDS |
| AAATAGACGAGCATGTTAAG+CGG | 0.653426 | 2.3:-48916163 | MS.gene051199:CDS |
| TTCAAAGTAACACTTCCAAG+TGG | 0.663392 | 2.3:+48924881 | None:intergenic |
| ATTAGTTTGGATATTCAAGG+AGG | 0.668285 | 2.3:-48924854 | MS.gene051199:CDS |
| ACTTATTCATAAAATGAAGG+AGG | 0.670389 | 2.3:-48909472 | MS.gene051199:intron |
| TTAGATATGAGATAATCTTG+TGG | 0.695616 | 2.3:+48916275 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 48908938 | 48925224 | 48908938 | ID=MS.gene051199 |
| chr2.3 | mRNA | 48908938 | 48925224 | 48908938 | ID=MS.gene051199.t1;Parent=MS.gene051199 |
| chr2.3 | exon | 48924797 | 48925224 | 48924797 | ID=MS.gene051199.t1.exon1;Parent=MS.gene051199.t1 |
| chr2.3 | CDS | 48924797 | 48925224 | 48924797 | ID=cds.MS.gene051199.t1;Parent=MS.gene051199.t1 |
| chr2.3 | exon | 48916118 | 48916553 | 48916118 | ID=MS.gene051199.t1.exon2;Parent=MS.gene051199.t1 |
| chr2.3 | CDS | 48916118 | 48916553 | 48916118 | ID=cds.MS.gene051199.t1;Parent=MS.gene051199.t1 |
| chr2.3 | exon | 48909473 | 48909553 | 48909473 | ID=MS.gene051199.t1.exon3;Parent=MS.gene051199.t1 |
| chr2.3 | CDS | 48909473 | 48909553 | 48909473 | ID=cds.MS.gene051199.t1;Parent=MS.gene051199.t1 |
| chr2.3 | exon | 48908938 | 48909009 | 48908938 | ID=MS.gene051199.t1.exon4;Parent=MS.gene051199.t1 |
| chr2.3 | CDS | 48908938 | 48909009 | 48908938 | ID=cds.MS.gene051199.t1;Parent=MS.gene051199.t1 |
| Gene Sequence |
| Protein sequence |
