Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60822.t1 | GAU12760.1 | 39 | 205 | 121 | 2 | 1 | 201 | 1 | 205 | 4.90E-27 | 131.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051122 | MS.gene60822 | 0.802679 | 5.00E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 49 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGAAATTCAAGGAGTTTC+TGG | 0.123756 | 1.4:-72797337 | None:intergenic |
| TATTGATTACTTGTCCTTTC+AGG | 0.165047 | 1.4:+72796993 | MS.gene60822:CDS |
| GTCGAAAAGATCTAAAATTT+TGG | 0.178634 | 1.4:+72796762 | MS.gene60822:CDS |
| TCCTTTGCCTTTCGAATTCA+TGG | 0.301017 | 1.4:+72796822 | MS.gene60822:CDS |
| GCTGTTGTATTGTAGATTAT+TGG | 0.305986 | 1.4:-72797091 | None:intergenic |
| GACAATACGAAAGCTCAAAT+TGG | 0.319470 | 1.4:+72797023 | MS.gene60822:CDS |
| AAGGAGGAGGAAGAGTTATT+TGG | 0.323852 | 1.4:-72796894 | None:intergenic |
| CCTCAAATGGTTCGAGAAAC+AGG | 0.344909 | 1.4:+72797254 | MS.gene60822:CDS |
| TAATCAATAATTTCCAAGAT+TGG | 0.354103 | 1.4:-72796979 | None:intergenic |
| AAGCTCAAATTGGCTAAGTT+TGG | 0.359836 | 1.4:+72797033 | MS.gene60822:CDS |
| ATTTGAGCTTTCGTATTGTC+AGG | 0.369492 | 1.4:-72797020 | None:intergenic |
| TCTTCGCAGACTTCCAATCT+TGG | 0.389719 | 1.4:+72796966 | MS.gene60822:CDS |
| TGGCTAAGTTTGGTGGATCT+TGG | 0.390395 | 1.4:+72797043 | MS.gene60822:CDS |
| CCTGTTTCTCGAACCATTTG+AGG | 0.415023 | 1.4:-72797254 | None:intergenic |
| CGCCATCTCGCATAAGGTGC+TGG | 0.424742 | 1.4:-72796795 | None:intergenic |
| CTCAAATTGGCTAAGTTTGG+TGG | 0.431809 | 1.4:+72797036 | MS.gene60822:CDS |
| GGTGATCGGTTGGTGATATT+CGG | 0.443933 | 1.4:+72797174 | MS.gene60822:CDS |
| GAGGCGGTAACGCAGGATCT+TGG | 0.445121 | 1.4:-72797235 | None:intergenic |
| GACCTTCGCCATCTCGCATA+AGG | 0.463108 | 1.4:-72796801 | None:intergenic |
| TCTTGGCACGCTCACTAGTA+AGG | 0.467933 | 1.4:-72797218 | None:intergenic |
| TTGCAGCTATTATTGAAAAG+AGG | 0.472869 | 1.4:+72797298 | MS.gene60822:CDS |
| TTCAATAATAGCTGCAAGCT+CGG | 0.476416 | 1.4:-72797292 | None:intergenic |
| CAGCGAACACCTTCTCCAGC+TGG | 0.484890 | 1.4:+72797110 | MS.gene60822:CDS |
| AGCGAACACCTTCTCCAGCT+GGG | 0.486800 | 1.4:+72797111 | MS.gene60822:CDS |
| TCCTGCGTTACCGCCTCAAA+TGG | 0.501071 | 1.4:+72797241 | MS.gene60822:CDS |
| CCTTTGCCTTTCGAATTCAT+GGG | 0.505515 | 1.4:+72796823 | MS.gene60822:CDS |
| ACCATTTGAGGCGGTAACGC+AGG | 0.520151 | 1.4:-72797242 | None:intergenic |
| CAGCATCGTGGGTGATCGGT+TGG | 0.552905 | 1.4:+72797164 | MS.gene60822:CDS |
| AGAGCAGCATCGTGGGTGAT+CGG | 0.565902 | 1.4:+72797160 | MS.gene60822:CDS |
| AGCATGATAAAAGAAATTCA+AGG | 0.569476 | 1.4:-72797346 | None:intergenic |
| AAGAGTGAGAGCAGCATCGT+GGG | 0.573141 | 1.4:+72797153 | MS.gene60822:CDS |
| TATTGTCAGGGTCACCTGAA+AGG | 0.575314 | 1.4:-72797007 | None:intergenic |
| CTATGAGACAGTATAATTCG+AGG | 0.577165 | 1.4:-72796939 | None:intergenic |
| CAACAATCTTCAGAAGGAGG+AGG | 0.580609 | 1.4:-72796907 | None:intergenic |
| TTTGAGCTTTCGTATTGTCA+GGG | 0.585321 | 1.4:-72797019 | None:intergenic |
| TTCCAGCACCTTATGCGAGA+TGG | 0.591021 | 1.4:+72796793 | MS.gene60822:CDS |
| CCCATGAATTCGAAAGGCAA+AGG | 0.595552 | 1.4:-72796823 | None:intergenic |
| GACTCGCAACAATCTTCAGA+AGG | 0.595638 | 1.4:-72796913 | None:intergenic |
| TTATCGCCCATGAATTCGAA+AGG | 0.600486 | 1.4:-72796829 | None:intergenic |
| GCGAACACCTTCTCCAGCTG+GGG | 0.603030 | 1.4:+72797112 | MS.gene60822:CDS |
| CACCTTATGCGAGATGGCGA+AGG | 0.605631 | 1.4:+72796799 | MS.gene60822:CDS |
| GGAGACATGCAGCAGAGAGG+TGG | 0.612152 | 1.4:+72797195 | MS.gene60822:CDS |
| TATTGGCTATGAGACTCTCG+AGG | 0.631466 | 1.4:-72797074 | None:intergenic |
| TCGCAACAATCTTCAGAAGG+AGG | 0.652776 | 1.4:-72796910 | None:intergenic |
| GTTTCTCGAACCATTTGAGG+CGG | 0.659651 | 1.4:-72797251 | None:intergenic |
| GAAGAGTGAGAGCAGCATCG+TGG | 0.660813 | 1.4:+72797152 | MS.gene60822:CDS |
| CGAACACCTTCTCCAGCTGG+GGG | 0.680093 | 1.4:+72797113 | MS.gene60822:CDS |
| ATTGGCTATGAGACTCTCGA+GGG | 0.685902 | 1.4:-72797073 | None:intergenic |
| TTCGGAGACATGCAGCAGAG+AGG | 0.705215 | 1.4:+72797192 | MS.gene60822:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATCAATAATTTCCAAGAT+TGG | - | chr1.4:72796982-72797001 | None:intergenic | 20.0% |
| ! | AGCATGATAAAAGAAATTCA+AGG | - | chr1.4:72797349-72797368 | None:intergenic | 25.0% |
| !!! | GTCGAAAAGATCTAAAATTT+TGG | + | chr1.4:72796762-72796781 | MS.gene60822:CDS | 25.0% |
| AAAGAAATTCAAGGAGTTTC+TGG | - | chr1.4:72797340-72797359 | None:intergenic | 30.0% | |
| CAATAAACTCTGCACAAAAA+CGG | - | chr1.4:72796861-72796880 | None:intergenic | 30.0% | |
| GCTGTTGTATTGTAGATTAT+TGG | - | chr1.4:72797094-72797113 | None:intergenic | 30.0% | |
| TTGCAGCTATTATTGAAAAG+AGG | + | chr1.4:72797298-72797317 | MS.gene60822:CDS | 30.0% | |
| ! | TATTGATTACTTGTCCTTTC+AGG | + | chr1.4:72796993-72797012 | MS.gene60822:CDS | 30.0% |
| AAGCTCAAATTGGCTAAGTT+TGG | + | chr1.4:72797033-72797052 | MS.gene60822:CDS | 35.0% | |
| ATTTGAGCTTTCGTATTGTC+AGG | - | chr1.4:72797023-72797042 | None:intergenic | 35.0% | |
| CTATGAGACAGTATAATTCG+AGG | - | chr1.4:72796942-72796961 | None:intergenic | 35.0% | |
| GACAATACGAAAGCTCAAAT+TGG | + | chr1.4:72797023-72797042 | MS.gene60822:CDS | 35.0% | |
| TTCAATAATAGCTGCAAGCT+CGG | - | chr1.4:72797295-72797314 | None:intergenic | 35.0% | |
| TTTGAGCTTTCGTATTGTCA+GGG | - | chr1.4:72797022-72797041 | None:intergenic | 35.0% | |
| AAGGAGGAGGAAGAGTTATT+TGG | - | chr1.4:72796897-72796916 | None:intergenic | 40.0% | |
| CCTTTGCCTTTCGAATTCAT+GGG | + | chr1.4:72796823-72796842 | MS.gene60822:CDS | 40.0% | |
| CTCAAATTGGCTAAGTTTGG+TGG | + | chr1.4:72797036-72797055 | MS.gene60822:CDS | 40.0% | |
| TCCTTTGCCTTTCGAATTCA+TGG | + | chr1.4:72796822-72796841 | MS.gene60822:CDS | 40.0% | |
| TTATCGCCCATGAATTCGAA+AGG | - | chr1.4:72796832-72796851 | None:intergenic | 40.0% | |
| ATTGGCTATGAGACTCTCGA+GGG | - | chr1.4:72797076-72797095 | None:intergenic | 45.0% | |
| CAACAATCTTCAGAAGGAGG+AGG | - | chr1.4:72796910-72796929 | None:intergenic | 45.0% | |
| CCCATGAATTCGAAAGGCAA+AGG | - | chr1.4:72796826-72796845 | None:intergenic | 45.0% | |
| CCTCAAATGGTTCGAGAAAC+AGG | + | chr1.4:72797254-72797273 | MS.gene60822:CDS | 45.0% | |
| GACTCGCAACAATCTTCAGA+AGG | - | chr1.4:72796916-72796935 | None:intergenic | 45.0% | |
| GTTTCTCGAACCATTTGAGG+CGG | - | chr1.4:72797254-72797273 | None:intergenic | 45.0% | |
| TATTGTCAGGGTCACCTGAA+AGG | - | chr1.4:72797010-72797029 | None:intergenic | 45.0% | |
| TCGCAACAATCTTCAGAAGG+AGG | - | chr1.4:72796913-72796932 | None:intergenic | 45.0% | |
| TCTTCGCAGACTTCCAATCT+TGG | + | chr1.4:72796966-72796985 | MS.gene60822:CDS | 45.0% | |
| ! | CCTGTTTCTCGAACCATTTG+AGG | - | chr1.4:72797257-72797276 | None:intergenic | 45.0% |
| ! | TATTGGCTATGAGACTCTCG+AGG | - | chr1.4:72797077-72797096 | None:intergenic | 45.0% |
| ! | TGGCTAAGTTTGGTGGATCT+TGG | + | chr1.4:72797043-72797062 | MS.gene60822:CDS | 45.0% |
| !! | GGTGATCGGTTGGTGATATT+CGG | + | chr1.4:72797174-72797193 | MS.gene60822:CDS | 45.0% |
| AAGAGTGAGAGCAGCATCGT+GGG | + | chr1.4:72797153-72797172 | MS.gene60822:CDS | 50.0% | |
| TCTTGGCACGCTCACTAGTA+AGG | - | chr1.4:72797221-72797240 | None:intergenic | 50.0% | |
| TTCCAGCACCTTATGCGAGA+TGG | + | chr1.4:72796793-72796812 | MS.gene60822:CDS | 50.0% | |
| AGAGCAGCATCGTGGGTGAT+CGG | + | chr1.4:72797160-72797179 | MS.gene60822:CDS | 55.0% | |
| AGCGAACACCTTCTCCAGCT+GGG | + | chr1.4:72797111-72797130 | MS.gene60822:CDS | 55.0% | |
| CACCTTATGCGAGATGGCGA+AGG | + | chr1.4:72796799-72796818 | MS.gene60822:CDS | 55.0% | |
| GAAGAGTGAGAGCAGCATCG+TGG | + | chr1.4:72797152-72797171 | MS.gene60822:CDS | 55.0% | |
| GACCTTCGCCATCTCGCATA+AGG | - | chr1.4:72796804-72796823 | None:intergenic | 55.0% | |
| TCCTGCGTTACCGCCTCAAA+TGG | + | chr1.4:72797241-72797260 | MS.gene60822:CDS | 55.0% | |
| TTCGGAGACATGCAGCAGAG+AGG | + | chr1.4:72797192-72797211 | MS.gene60822:CDS | 55.0% | |
| ! | ACCATTTGAGGCGGTAACGC+AGG | - | chr1.4:72797245-72797264 | None:intergenic | 55.0% |
| ! | CATTTTCTCGATCCCCCAGC+TGG | - | chr1.4:72797128-72797147 | None:intergenic | 55.0% |
| CAGCATCGTGGGTGATCGGT+TGG | + | chr1.4:72797164-72797183 | MS.gene60822:CDS | 60.0% | |
| CAGCGAACACCTTCTCCAGC+TGG | + | chr1.4:72797110-72797129 | MS.gene60822:CDS | 60.0% | |
| CGAACACCTTCTCCAGCTGG+GGG | + | chr1.4:72797113-72797132 | MS.gene60822:CDS | 60.0% | |
| CGCCATCTCGCATAAGGTGC+TGG | - | chr1.4:72796798-72796817 | None:intergenic | 60.0% | |
| GAGGCGGTAACGCAGGATCT+TGG | - | chr1.4:72797238-72797257 | None:intergenic | 60.0% | |
| GCGAACACCTTCTCCAGCTG+GGG | + | chr1.4:72797112-72797131 | MS.gene60822:CDS | 60.0% | |
| GGAGACATGCAGCAGAGAGG+TGG | + | chr1.4:72797195-72797214 | MS.gene60822:CDS | 60.0% | |
| CTCGATCCCCCAGCTGGAGA+AGG | - | chr1.4:72797122-72797141 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 72796760 | 72797371 | 72796760 | ID=MS.gene60822 |
| chr1.4 | mRNA | 72796760 | 72797371 | 72796760 | ID=MS.gene60822.t1;Parent=MS.gene60822 |
| chr1.4 | exon | 72796760 | 72797371 | 72796760 | ID=MS.gene60822.t1.exon1;Parent=MS.gene60822.t1 |
| chr1.4 | CDS | 72796760 | 72797371 | 72796760 | ID=cds.MS.gene60822.t1;Parent=MS.gene60822.t1 |
| Gene Sequence |
| Protein sequence |
