Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61171.t1 | XP_003606598.2 | 96.1 | 593 | 22 | 1 | 1 | 592 | 38 | 630 | 4.30E-305 | 1056.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61171.t1 | Q8VY27 | 42.8 | 579 | 308 | 6 | 1 | 566 | 42 | 610 | 2.3e-121 | 437.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61171.t1 | G7JTM6 | 96.1 | 593 | 22 | 1 | 1 | 592 | 38 | 630 | 3.1e-305 | 1056.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050363 | MS.gene61171 | 0.807497 | 4.91E-50 | -1.69E-46 |
| MS.gene060286 | MS.gene61171 | 0.812977 | 3.23E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61171.t1 | MTR_4g062330 | 96.290 | 593 | 21 | 1 | 1 | 592 | 38 | 630 | 0.0 | 1187 |
| MS.gene61171.t1 | MTR_2g096230 | 58.639 | 573 | 211 | 9 | 1 | 569 | 34 | 584 | 0.0 | 622 |
| MS.gene61171.t1 | MTR_1g067480 | 46.447 | 577 | 276 | 10 | 5 | 565 | 42 | 601 | 9.52e-169 | 493 |
| MS.gene61171.t1 | MTR_1g067460 | 47.491 | 558 | 274 | 9 | 5 | 556 | 40 | 584 | 4.39e-165 | 484 |
| MS.gene61171.t1 | MTR_8g021330 | 31.058 | 586 | 362 | 11 | 7 | 557 | 11 | 589 | 4.06e-92 | 296 |
| MS.gene61171.t1 | MTR_4g103540 | 32.212 | 565 | 330 | 13 | 39 | 566 | 132 | 680 | 2.02e-86 | 283 |
| MS.gene61171.t1 | MTR_5g073450 | 29.610 | 564 | 365 | 8 | 25 | 569 | 86 | 636 | 9.13e-82 | 270 |
| MS.gene61171.t1 | MTR_1g090620 | 28.975 | 566 | 342 | 9 | 37 | 566 | 100 | 641 | 1.08e-78 | 261 |
| MS.gene61171.t1 | MTR_7g013770 | 28.829 | 555 | 366 | 10 | 35 | 566 | 118 | 666 | 1.52e-77 | 259 |
| MS.gene61171.t1 | MTR_3g031130 | 33.258 | 442 | 269 | 5 | 143 | 574 | 201 | 626 | 1.88e-76 | 255 |
| MS.gene61171.t1 | MTR_1g057560 | 31.808 | 437 | 253 | 7 | 144 | 561 | 210 | 620 | 4.98e-73 | 246 |
| MS.gene61171.t1 | MTR_6g033310 | 27.099 | 524 | 359 | 7 | 56 | 566 | 212 | 725 | 3.98e-67 | 232 |
| MS.gene61171.t1 | MTR_3g025900 | 29.205 | 541 | 359 | 10 | 39 | 566 | 79 | 608 | 2.16e-66 | 228 |
| MS.gene61171.t1 | MTR_7g026630 | 28.023 | 521 | 344 | 13 | 65 | 566 | 142 | 650 | 1.48e-60 | 213 |
| MS.gene61171.t1 | MTR_5g073460 | 28.294 | 463 | 311 | 6 | 115 | 566 | 240 | 692 | 7.01e-56 | 201 |
| MS.gene61171.t1 | MTR_1g117090 | 24.838 | 463 | 309 | 8 | 144 | 568 | 209 | 670 | 4.01e-45 | 170 |
| MS.gene61171.t1 | MTR_5g073620 | 29.231 | 325 | 199 | 3 | 143 | 465 | 297 | 592 | 8.18e-41 | 159 |
| MS.gene61171.t1 | MTR_5g073620 | 31.579 | 152 | 99 | 3 | 409 | 557 | 903 | 1052 | 1.91e-13 | 74.3 |
| MS.gene61171.t1 | MTR_5g073480 | 27.333 | 300 | 208 | 2 | 119 | 418 | 245 | 534 | 6.21e-35 | 140 |
| MS.gene61171.t1 | MTR_5g074130 | 24.083 | 436 | 292 | 9 | 144 | 564 | 262 | 673 | 1.37e-31 | 130 |
| MS.gene61171.t1 | MTR_1g009620 | 24.365 | 394 | 266 | 6 | 159 | 541 | 248 | 620 | 5.41e-28 | 119 |
| MS.gene61171.t1 | MTR_5g073920 | 24.444 | 405 | 269 | 10 | 141 | 539 | 253 | 626 | 2.59e-27 | 117 |
| MS.gene61171.t1 | MTR_3g464010 | 23.239 | 426 | 296 | 8 | 123 | 542 | 238 | 638 | 3.77e-26 | 114 |
| MS.gene61171.t1 | MTR_4g008260 | 23.438 | 384 | 269 | 6 | 144 | 524 | 227 | 588 | 1.39e-22 | 102 |
| MS.gene61171.t1 | MTR_5g093530 | 23.370 | 368 | 233 | 7 | 205 | 564 | 244 | 570 | 2.35e-22 | 101 |
| MS.gene61171.t1 | MTR_1g017910 | 23.347 | 484 | 328 | 16 | 121 | 577 | 233 | 700 | 1.04e-20 | 97.1 |
| MS.gene61171.t1 | MTR_5g073770 | 28.090 | 178 | 125 | 2 | 143 | 318 | 210 | 386 | 2.09e-19 | 90.9 |
| MS.gene61171.t1 | MTR_5g073900 | 31.250 | 192 | 118 | 6 | 151 | 340 | 1 | 180 | 1.37e-16 | 82.0 |
| MS.gene61171.t1 | MTR_5g073780 | 25.764 | 229 | 136 | 5 | 332 | 557 | 10 | 207 | 4.84e-15 | 75.1 |
| MS.gene61171.t1 | MTR_5g073960 | 27.485 | 171 | 109 | 4 | 118 | 288 | 242 | 397 | 2.53e-12 | 69.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61171.t1 | AT5G59730 | 47.708 | 589 | 269 | 9 | 1 | 566 | 26 | 598 | 2.80e-180 | 524 |
| MS.gene61171.t1 | AT5G59730 | 47.708 | 589 | 269 | 9 | 1 | 566 | 26 | 598 | 2.99e-180 | 524 |
| MS.gene61171.t1 | AT3G09520 | 46.472 | 581 | 271 | 11 | 5 | 564 | 43 | 604 | 8.03e-166 | 486 |
| MS.gene61171.t1 | AT2G39380 | 43.793 | 580 | 301 | 9 | 1 | 566 | 42 | 610 | 2.41e-162 | 478 |
| MS.gene61171.t1 | AT1G07725 | 44.464 | 578 | 293 | 9 | 3 | 567 | 30 | 592 | 9.70e-160 | 471 |
| MS.gene61171.t1 | AT2G28640 | 45.000 | 560 | 282 | 9 | 31 | 587 | 62 | 598 | 1.79e-159 | 469 |
| MS.gene61171.t1 | AT3G55150 | 45.611 | 581 | 289 | 10 | 1 | 566 | 42 | 610 | 1.76e-155 | 460 |
| MS.gene61171.t1 | AT3G09530 | 41.283 | 608 | 322 | 10 | 3 | 591 | 36 | 627 | 2.29e-149 | 445 |
| MS.gene61171.t1 | AT2G28650 | 45.455 | 572 | 260 | 7 | 1 | 565 | 29 | 555 | 3.10e-145 | 432 |
| MS.gene61171.t1 | AT5G50380 | 30.732 | 615 | 346 | 12 | 7 | 566 | 82 | 671 | 2.22e-90 | 293 |
| MS.gene61171.t1 | AT3G14090 | 29.489 | 607 | 377 | 8 | 4 | 566 | 14 | 613 | 1.25e-89 | 290 |
| MS.gene61171.t1 | AT1G72470 | 30.241 | 582 | 347 | 11 | 7 | 544 | 25 | 591 | 1.23e-84 | 277 |
| MS.gene61171.t1 | AT1G54090 | 29.173 | 617 | 369 | 11 | 1 | 568 | 9 | 606 | 7.77e-84 | 274 |
| MS.gene61171.t1 | AT5G03540 | 29.084 | 557 | 339 | 9 | 39 | 566 | 101 | 630 | 6.22e-78 | 259 |
| MS.gene61171.t1 | AT5G52340 | 28.624 | 545 | 350 | 8 | 39 | 566 | 101 | 623 | 3.39e-76 | 254 |
| MS.gene61171.t1 | AT5G03540 | 29.159 | 535 | 326 | 8 | 58 | 566 | 8 | 515 | 2.25e-75 | 249 |
| MS.gene61171.t1 | AT5G58430 | 30.534 | 524 | 328 | 7 | 63 | 570 | 119 | 622 | 5.16e-75 | 251 |
| MS.gene61171.t1 | AT5G03540 | 28.152 | 579 | 342 | 9 | 39 | 566 | 101 | 656 | 5.01e-74 | 249 |
| MS.gene61171.t1 | AT1G07000 | 29.317 | 498 | 303 | 8 | 65 | 553 | 120 | 577 | 6.52e-73 | 245 |
| MS.gene61171.t1 | AT3G29400 | 28.947 | 570 | 370 | 10 | 35 | 577 | 93 | 654 | 1.05e-72 | 246 |
| MS.gene61171.t1 | AT5G61010 | 29.220 | 551 | 347 | 12 | 35 | 564 | 96 | 624 | 9.96e-66 | 226 |
| MS.gene61171.t1 | AT5G61010 | 29.220 | 551 | 347 | 12 | 35 | 564 | 96 | 624 | 9.96e-66 | 226 |
| MS.gene61171.t1 | AT5G13990 | 28.625 | 531 | 337 | 13 | 57 | 566 | 172 | 681 | 3.33e-61 | 215 |
| MS.gene61171.t1 | AT5G13150 | 28.096 | 541 | 336 | 14 | 57 | 566 | 126 | 644 | 2.34e-59 | 209 |
| MS.gene61171.t1 | AT5G52350 | 28.670 | 436 | 276 | 6 | 144 | 566 | 164 | 577 | 9.54e-56 | 198 |
| MS.gene61171.t1 | AT4G31540 | 24.112 | 394 | 267 | 6 | 159 | 541 | 251 | 623 | 6.41e-28 | 119 |
| MS.gene61171.t1 | AT1G51640 | 24.129 | 402 | 261 | 7 | 160 | 541 | 215 | 592 | 3.38e-24 | 107 |
Find 107 sgRNAs with CRISPR-Local
Find 129 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGGTTGTTGGTGGAGATTC+AGG | 0.119175 | 4.4:+43068741 | MS.gene61171:CDS |
| CTCTATTTGTTGAGAGTATT+TGG | 0.171101 | 4.4:-43067291 | None:intergenic |
| CTCTCTCCGTTTCCGAGATT+TGG | 0.213889 | 4.4:-43067429 | None:intergenic |
| GTTCTCTCAGATTCAGAAAA+TGG | 0.216099 | 4.4:+43067600 | MS.gene61171:CDS |
| GGAATATATAGAGAGTTATT+TGG | 0.230887 | 4.4:+43068704 | MS.gene61171:CDS |
| TAATCTCATCCCTTAGTTTC+GGG | 0.269257 | 4.4:-43068596 | None:intergenic |
| TTAATCTCATCCCTTAGTTT+CGG | 0.281842 | 4.4:-43068597 | None:intergenic |
| AAGAGACATAGCACCTTCTT+TGG | 0.293775 | 4.4:-43067773 | None:intergenic |
| GAACGGTTAGAAACGGATTC+AGG | 0.295501 | 4.4:-43067325 | None:intergenic |
| AGCTGTTAAAACGATGTTAA+AGG | 0.314282 | 4.4:+43067972 | MS.gene61171:CDS |
| GTGAACAGATTTGTTCAAAT+TGG | 0.315184 | 4.4:+43068429 | MS.gene61171:CDS |
| GAGTTTCTCCATTGAATTAA+CGG | 0.334263 | 4.4:-43067944 | None:intergenic |
| TTGAATTAACGGCTTGTAAA+CGG | 0.336520 | 4.4:-43067933 | None:intergenic |
| AGCGAGAATTCCACCGTAAT+CGG | 0.337949 | 4.4:-43068097 | None:intergenic |
| ATAACCTTTCTCGCCGATTA+CGG | 0.356904 | 4.4:+43068084 | MS.gene61171:CDS |
| GGGTTGTTGGTGGAGATTCA+GGG | 0.367290 | 4.4:+43068742 | MS.gene61171:CDS |
| GTAAACCCTTGACAGCATTA+AGG | 0.367474 | 4.4:-43067162 | None:intergenic |
| GATGAAGCACTTTATCATCT+TGG | 0.370056 | 4.4:+43067562 | MS.gene61171:CDS |
| GATGAGATTAAAGCGTTGAT+TGG | 0.379517 | 4.4:+43068609 | MS.gene61171:CDS |
| CAGAATCACTTATAACCAAA+TGG | 0.381971 | 4.4:+43067068 | MS.gene61171:CDS |
| TTTACAAGCCGTTAATTCAA+TGG | 0.396075 | 4.4:+43067936 | MS.gene61171:CDS |
| GATGACGGTGGAGCAGGTTA+AGG | 0.396814 | 4.4:+43068503 | MS.gene61171:CDS |
| GAATATATAGAGAGTTATTT+GGG | 0.414543 | 4.4:+43068705 | MS.gene61171:CDS |
| GAGAAGAATCGGCTCGGGTC+CGG | 0.423586 | 4.4:+43068666 | MS.gene61171:CDS |
| GTTGATTATTTGGTTTGTGT+TGG | 0.431217 | 4.4:-43067098 | None:intergenic |
| TTCCGGTTCATATCTTAAAC+CGG | 0.437962 | 4.4:-43068685 | None:intergenic |
| TGTGTTGGAATAATCCCATT+TGG | 0.443652 | 4.4:-43067083 | None:intergenic |
| CTCAGATTCAGAAAATGGAT+TGG | 0.447224 | 4.4:+43067605 | MS.gene61171:CDS |
| TCCACCGTAATCGGCGAGAA+AGG | 0.447422 | 4.4:-43068088 | None:intergenic |
| GGAAACTTCATCATCGTAAT+CGG | 0.448881 | 4.4:-43067380 | None:intergenic |
| AGTGTAAGAAACAAACTTCA+TGG | 0.462527 | 4.4:+43068562 | MS.gene61171:CDS |
| ACAGATTTGTTCAAATTGGA+TGG | 0.467517 | 4.4:+43068433 | MS.gene61171:CDS |
| TTGTATCCGACCCGAAACTA+AGG | 0.469300 | 4.4:+43068586 | MS.gene61171:CDS |
| CATGCAATACGTCGTCGTAA+AGG | 0.469491 | 4.4:+43068332 | MS.gene61171:CDS |
| GACGACGTGTTCGCCGCCGC+AGG | 0.471052 | 4.4:+43067715 | MS.gene61171:CDS |
| GTCCGGTTTAAGATATGAAC+CGG | 0.475745 | 4.4:+43068683 | MS.gene61171:CDS |
| GGTTTCTTCTCGGAGAAGAA+TGG | 0.476609 | 4.4:+43068373 | MS.gene61171:CDS |
| AAGGTTCGAAGATCGAATCT+AGG | 0.481716 | 4.4:+43068351 | MS.gene61171:CDS |
| TGCATGGTAAACTGTGGGTA+TGG | 0.484151 | 4.4:+43067493 | MS.gene61171:CDS |
| GACAGCATTAAGGTAGTGCT+TGG | 0.485405 | 4.4:-43067152 | None:intergenic |
| TATGCCGCAATCTCCGTTAC+CGG | 0.487493 | 4.4:+43068134 | MS.gene61171:CDS |
| GTTGATTGGTTCGAAGTTGG+TGG | 0.489505 | 4.4:+43068623 | MS.gene61171:CDS |
| GAGAGTTTCCATGCTTGCTA+TGG | 0.489637 | 4.4:+43067447 | MS.gene61171:CDS |
| TCAGATTCAGAAAATGGATT+GGG | 0.489741 | 4.4:+43067606 | MS.gene61171:CDS |
| AGCTCTTACAAGAGTGTTGG+AGG | 0.489943 | 4.4:-43067221 | None:intergenic |
| TTCGCAGAGATTACCAAAGA+AGG | 0.493577 | 4.4:+43067760 | MS.gene61171:CDS |
| GTGGATTCTATGAGAAGAAT+CGG | 0.493647 | 4.4:+43068655 | MS.gene61171:CDS |
| TTGAGCTCTTACAAGAGTGT+TGG | 0.495027 | 4.4:-43067224 | None:intergenic |
| CGTTTCTAACCGTTCTTCAA+CGG | 0.498578 | 4.4:+43067333 | MS.gene61171:CDS |
| ATGACGGTGGAGCAGGTTAA+GGG | 0.499266 | 4.4:+43068504 | MS.gene61171:CDS |
| TCGAATCTAGGGTTTCTTCT+CGG | 0.502075 | 4.4:+43068363 | MS.gene61171:CDS |
| TCGAAGTTGGTGGCGAAGTA+CGG | 0.502714 | 4.4:+43068633 | MS.gene61171:CDS |
| AGAATCACTTATAACCAAAT+GGG | 0.512386 | 4.4:+43067069 | MS.gene61171:CDS |
| AACGGTTAGAAACGGATTCA+GGG | 0.513535 | 4.4:-43067324 | None:intergenic |
| TTCATCATCGTAATCGGAAA+CGG | 0.513891 | 4.4:-43067374 | None:intergenic |
| GACGGAGATGACGGTGGAGC+AGG | 0.515839 | 4.4:+43068497 | MS.gene61171:CDS |
| CGATAGTACGACTCCGGTAA+CGG | 0.518318 | 4.4:-43068147 | None:intergenic |
| AGCGTTGATTGGTTCGAAGT+TGG | 0.520695 | 4.4:+43068620 | MS.gene61171:CDS |
| GAAAATCAAGACGTGGTTGA+AGG | 0.527556 | 4.4:+43067642 | MS.gene61171:CDS |
| GTTAACGAATCATGAATTGA+AGG | 0.537316 | 4.4:+43068395 | MS.gene61171:CDS |
| GCACTACCTTAATGCTGTCA+AGG | 0.537818 | 4.4:+43067156 | MS.gene61171:CDS |
| AAATAACTCTCTATATATTC+CGG | 0.538767 | 4.4:-43068702 | None:intergenic |
| CGTTGAAGAACGGTTAGAAA+CGG | 0.538885 | 4.4:-43067332 | None:intergenic |
| AGTTTCCATGCTTGCTATGG+CGG | 0.539595 | 4.4:+43067450 | MS.gene61171:CDS |
| AGGTTCGAAGATCGAATCTA+GGG | 0.540290 | 4.4:+43068352 | MS.gene61171:CDS |
| TTCTATGAGAAGAATCGGCT+CGG | 0.541073 | 4.4:+43068660 | MS.gene61171:CDS |
| ACGACGTGTTCGCCGCCGCA+GGG | 0.548048 | 4.4:+43067716 | MS.gene61171:CDS |
| CAAATCCGCCATAGCAAGCA+TGG | 0.549061 | 4.4:-43067455 | None:intergenic |
| CACTACCTTAATGCTGTCAA+GGG | 0.552203 | 4.4:+43067157 | MS.gene61171:CDS |
| TGTTCAAATTGGATGGAACA+AGG | 0.553685 | 4.4:+43068440 | MS.gene61171:CDS |
| TTCACGGGCGAATAGAGCTA+AGG | 0.555917 | 4.4:-43068803 | None:intergenic |
| ACCTTTCTCGCCGATTACGG+TGG | 0.562892 | 4.4:+43068087 | MS.gene61171:CDS |
| TTCGACGATCCGTTGAAGAA+CGG | 0.564288 | 4.4:-43067342 | None:intergenic |
| CGGAAACCAAATCTCGGAAA+CGG | 0.565126 | 4.4:+43067423 | MS.gene61171:CDS |
| GTATGGGAAAGAGTGTGTTA+AGG | 0.568103 | 4.4:+43067510 | MS.gene61171:CDS |
| CGCAGAGAATTCTTTCGCCG+TGG | 0.572907 | 4.4:-43067693 | None:intergenic |
| GAAAGCTATTGCTGATTGCA+TGG | 0.572953 | 4.4:+43067477 | MS.gene61171:CDS |
| GATGAATTCTCGCTGCGCAT+CGG | 0.573377 | 4.4:-43068174 | None:intergenic |
| AAATTCCACGACGGAGATGA+CGG | 0.573888 | 4.4:+43068488 | MS.gene61171:CDS |
| GTGTAAGAAACAAACTTCAT+GGG | 0.576303 | 4.4:+43068563 | MS.gene61171:CDS |
| CTCATCCCTTAGTTTCGGGT+CGG | 0.579411 | 4.4:-43068592 | None:intergenic |
| GCATGGTAAACTGTGGGTAT+GGG | 0.579914 | 4.4:+43067494 | MS.gene61171:CDS |
| AAGTTGGTGGCGAAGTACGG+TGG | 0.586585 | 4.4:+43068636 | MS.gene61171:CDS |
| CGTCGTCGGAAACCAAATCT+CGG | 0.598144 | 4.4:+43067417 | MS.gene61171:CDS |
| GACTCCGGTAACGGAGATTG+CGG | 0.599424 | 4.4:-43068138 | None:intergenic |
| CTCCACCGTCATCTCCGTCG+TGG | 0.600419 | 4.4:-43068493 | None:intergenic |
| GCCGTGGAAAAGTGTTCCCA+CGG | 0.601586 | 4.4:-43067677 | None:intergenic |
| TTCATGACATAGCGTGTGAG+AGG | 0.605604 | 4.4:-43068057 | None:intergenic |
| TCACGGGCGAATAGAGCTAA+GGG | 0.607170 | 4.4:-43068802 | None:intergenic |
| TCTTACAAGAGTGTTGGAGG+TGG | 0.608949 | 4.4:-43067218 | None:intergenic |
| ATCGTCGTCATCATCTTCGT+CGG | 0.613159 | 4.4:+43068200 | MS.gene61171:CDS |
| TGTATCCGACCCGAAACTAA+GGG | 0.621062 | 4.4:+43068587 | MS.gene61171:CDS |
| AACGGCTTGTAAACGGACGT+TGG | 0.621135 | 4.4:-43067926 | None:intergenic |
| GAGAGGATGAACACCACCAC+CGG | 0.624202 | 4.4:-43068040 | None:intergenic |
| TCTATGAGAAGAATCGGCTC+GGG | 0.625352 | 4.4:+43068661 | MS.gene61171:CDS |
| GGCGATTCTCTTCCCTGCGG+CGG | 0.633416 | 4.4:-43067728 | None:intergenic |
| GAAGGCTGTTAAAGTCGCCG+TGG | 0.638922 | 4.4:+43067660 | MS.gene61171:CDS |
| AAGGCTGTTAAAGTCGCCGT+GGG | 0.643890 | 4.4:+43067661 | MS.gene61171:CDS |
| AATCTCTGCGAAACAAGACT+CGG | 0.657686 | 4.4:-43067749 | None:intergenic |
| CTGATTGCATGGTAAACTGT+GGG | 0.666497 | 4.4:+43067488 | MS.gene61171:CDS |
| TTGAGTTGAAAATCAAGACG+TGG | 0.674843 | 4.4:+43067635 | MS.gene61171:CDS |
| TTCCACGACGGAGATGACGG+TGG | 0.677099 | 4.4:+43068491 | MS.gene61171:CDS |
| GGACTTCGATAGTACGACTC+CGG | 0.684693 | 4.4:-43068153 | None:intergenic |
| GCTGATTGCATGGTAAACTG+TGG | 0.727746 | 4.4:+43067487 | MS.gene61171:CDS |
| GGGAAATGAAAATTCCACGA+CGG | 0.733957 | 4.4:+43068479 | MS.gene61171:CDS |
| AGATATCAGAGAAAATCGCG+TGG | 0.736405 | 4.4:+43068223 | MS.gene61171:CDS |
| CTCGGCGATTCTCTTCCCTG+CGG | 0.764642 | 4.4:-43067731 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATAACTCTCTATATATTC+CGG | - | chr4.4:43068705-43068724 | None:intergenic | 20.0% |
| !! | GAATATATAGAGAGTTATTT+GGG | + | chr4.4:43068705-43068724 | MS.gene61171:CDS | 20.0% |
| !!! | ATTTGGGTAATATTTTGTAT+GGG | + | chr4.4:43068721-43068740 | MS.gene61171:CDS | 20.0% |
| !!! | TATTTGGGTAATATTTTGTA+TGG | + | chr4.4:43068720-43068739 | MS.gene61171:CDS | 20.0% |
| ! | AGAATCACTTATAACCAAAT+GGG | + | chr4.4:43067069-43067088 | MS.gene61171:CDS | 25.0% |
| ! | GGAATATATAGAGAGTTATT+TGG | + | chr4.4:43068704-43068723 | MS.gene61171:CDS | 25.0% |
| !! | TTTGGGTAATATTTTGTATG+GGG | + | chr4.4:43068722-43068741 | MS.gene61171:CDS | 25.0% |
| !!! | TAAAAAGTGGGTTGATTATT+TGG | - | chr4.4:43067111-43067130 | None:intergenic | 25.0% |
| !!! | TATGTCTCTTTTTACTTTTC+CGG | + | chr4.4:43067786-43067805 | MS.gene61171:CDS | 25.0% |
| AGCTGTTAAAACGATGTTAA+AGG | + | chr4.4:43067972-43067991 | MS.gene61171:CDS | 30.0% | |
| AGTGTAAGAAACAAACTTCA+TGG | + | chr4.4:43068562-43068581 | MS.gene61171:CDS | 30.0% | |
| CAGAATCACTTATAACCAAA+TGG | + | chr4.4:43067068-43067087 | MS.gene61171:CDS | 30.0% | |
| GAGTTTCTCCATTGAATTAA+CGG | - | chr4.4:43067947-43067966 | None:intergenic | 30.0% | |
| GTGTAAGAAACAAACTTCAT+GGG | + | chr4.4:43068563-43068582 | MS.gene61171:CDS | 30.0% | |
| GTTAACGAATCATGAATTGA+AGG | + | chr4.4:43068395-43068414 | MS.gene61171:CDS | 30.0% | |
| TCTTCAAAGAAAAAAGTCTC+CGG | + | chr4.4:43068021-43068040 | MS.gene61171:CDS | 30.0% | |
| TTAATCTCATCCCTTAGTTT+CGG | - | chr4.4:43068600-43068619 | None:intergenic | 30.0% | |
| TTGAATTAACGGCTTGTAAA+CGG | - | chr4.4:43067936-43067955 | None:intergenic | 30.0% | |
| TTTACAAGCCGTTAATTCAA+TGG | + | chr4.4:43067936-43067955 | MS.gene61171:CDS | 30.0% | |
| ! | AATATTTTGTATGGGGTTGT+TGG | + | chr4.4:43068729-43068748 | MS.gene61171:CDS | 30.0% |
| ! | ACAGATTTGTTCAAATTGGA+TGG | + | chr4.4:43068433-43068452 | MS.gene61171:CDS | 30.0% |
| ! | GTGAACAGATTTGTTCAAAT+TGG | + | chr4.4:43068429-43068448 | MS.gene61171:CDS | 30.0% |
| ! | TCAGATTCAGAAAATGGATT+GGG | + | chr4.4:43067606-43067625 | MS.gene61171:CDS | 30.0% |
| !! | CTCTATTTGTTGAGAGTATT+TGG | - | chr4.4:43067294-43067313 | None:intergenic | 30.0% |
| !! | GTTGATTATTTGGTTTGTGT+TGG | - | chr4.4:43067101-43067120 | None:intergenic | 30.0% |
| !!! | ATAATCAACCCACTTTTTAG+CGG | + | chr4.4:43067112-43067131 | MS.gene61171:CDS | 30.0% |
| CTCAGATTCAGAAAATGGAT+TGG | + | chr4.4:43067605-43067624 | MS.gene61171:CDS | 35.0% | |
| GGAAACTTCATCATCGTAAT+CGG | - | chr4.4:43067383-43067402 | None:intergenic | 35.0% | |
| GTGGATTCTATGAGAAGAAT+CGG | + | chr4.4:43068655-43068674 | MS.gene61171:CDS | 35.0% | |
| GTTCTCTCAGATTCAGAAAA+TGG | + | chr4.4:43067600-43067619 | MS.gene61171:CDS | 35.0% | |
| TAATCTCATCCCTTAGTTTC+GGG | - | chr4.4:43068599-43068618 | None:intergenic | 35.0% | |
| TGTGTTGGAATAATCCCATT+TGG | - | chr4.4:43067086-43067105 | None:intergenic | 35.0% | |
| TGTTCAAATTGGATGGAACA+AGG | + | chr4.4:43068440-43068459 | MS.gene61171:CDS | 35.0% | |
| TTCATCATCGTAATCGGAAA+CGG | - | chr4.4:43067377-43067396 | None:intergenic | 35.0% | |
| TTCCGGTTCATATCTTAAAC+CGG | - | chr4.4:43068688-43068707 | None:intergenic | 35.0% | |
| ! | GATGAAGCACTTTATCATCT+TGG | + | chr4.4:43067562-43067581 | MS.gene61171:CDS | 35.0% |
| !! | AGTGTTCTGAACATTTTCTC+AGG | - | chr4.4:43067838-43067857 | None:intergenic | 35.0% |
| !! | GATGAGATTAAAGCGTTGAT+TGG | + | chr4.4:43068609-43068628 | MS.gene61171:CDS | 35.0% |
| !! | TCTTTTTACTTTTCCGGACA+TGG | + | chr4.4:43067792-43067811 | MS.gene61171:CDS | 35.0% |
| !! | TTGAGTTGAAAATCAAGACG+TGG | + | chr4.4:43067635-43067654 | MS.gene61171:CDS | 35.0% |
| AAGAGACATAGCACCTTCTT+TGG | - | chr4.4:43067776-43067795 | None:intergenic | 40.0% | |
| AAGGTTCGAAGATCGAATCT+AGG | + | chr4.4:43068351-43068370 | MS.gene61171:CDS | 40.0% | |
| AATCTCTGCGAAACAAGACT+CGG | - | chr4.4:43067752-43067771 | None:intergenic | 40.0% | |
| AGATATCAGAGAAAATCGCG+TGG | + | chr4.4:43068223-43068242 | MS.gene61171:CDS | 40.0% | |
| AGGTTCGAAGATCGAATCTA+GGG | + | chr4.4:43068352-43068371 | MS.gene61171:CDS | 40.0% | |
| CACTACCTTAATGCTGTCAA+GGG | + | chr4.4:43067157-43067176 | MS.gene61171:CDS | 40.0% | |
| CGTTGAAGAACGGTTAGAAA+CGG | - | chr4.4:43067335-43067354 | None:intergenic | 40.0% | |
| CGTTTCTAACCGTTCTTCAA+CGG | + | chr4.4:43067333-43067352 | MS.gene61171:CDS | 40.0% | |
| CTGATTGCATGGTAAACTGT+GGG | + | chr4.4:43067488-43067507 | MS.gene61171:CDS | 40.0% | |
| GAAAATCAAGACGTGGTTGA+AGG | + | chr4.4:43067642-43067661 | MS.gene61171:CDS | 40.0% | |
| GACGATGAAAAATTCGTCGT+CGG | + | chr4.4:43067403-43067422 | MS.gene61171:CDS | 40.0% | |
| GAGTGTTACCGCTAAAAAGT+GGG | - | chr4.4:43067123-43067142 | None:intergenic | 40.0% | |
| GGGAAATGAAAATTCCACGA+CGG | + | chr4.4:43068479-43068498 | MS.gene61171:CDS | 40.0% | |
| GTAAACCCTTGACAGCATTA+AGG | - | chr4.4:43067165-43067184 | None:intergenic | 40.0% | |
| TCAAAGAAAAAAGTCTCCGG+TGG | + | chr4.4:43068024-43068043 | MS.gene61171:CDS | 40.0% | |
| TTCGCAGAGATTACCAAAGA+AGG | + | chr4.4:43067760-43067779 | MS.gene61171:CDS | 40.0% | |
| TTCTATGAGAAGAATCGGCT+CGG | + | chr4.4:43068660-43068679 | MS.gene61171:CDS | 40.0% | |
| ! | AACGGTTAGAAACGGATTCA+GGG | - | chr4.4:43067327-43067346 | None:intergenic | 40.0% |
| ! | ATAACCTTTCTCGCCGATTA+CGG | + | chr4.4:43068084-43068103 | MS.gene61171:CDS | 40.0% |
| ! | ATTTTGTATGGGGTTGTTGG+TGG | + | chr4.4:43068732-43068751 | MS.gene61171:CDS | 40.0% |
| ! | GAAAGCTATTGCTGATTGCA+TGG | + | chr4.4:43067477-43067496 | MS.gene61171:CDS | 40.0% |
| ! | GACGACGAATTTTTCATCGT+CGG | - | chr4.4:43067404-43067423 | None:intergenic | 40.0% |
| ! | GTATGGGAAAGAGTGTGTTA+AGG | + | chr4.4:43067510-43067529 | MS.gene61171:CDS | 40.0% |
| ! | GTCCGGTTTAAGATATGAAC+CGG | + | chr4.4:43068683-43068702 | MS.gene61171:CDS | 40.0% |
| ! | GTCGTGGAATTTTCATTTCC+CGG | - | chr4.4:43068480-43068499 | None:intergenic | 40.0% |
| ! | TCGAATCTAGGGTTTCTTCT+CGG | + | chr4.4:43068363-43068382 | MS.gene61171:CDS | 40.0% |
| ! | TTGAGCTCTTACAAGAGTGT+TGG | - | chr4.4:43067227-43067246 | None:intergenic | 40.0% |
| !! | TTTTACTTTTCCGGACATGG+TGG | + | chr4.4:43067795-43067814 | MS.gene61171:CDS | 40.0% |
| AAATTCCACGACGGAGATGA+CGG | + | chr4.4:43068488-43068507 | MS.gene61171:CDS | 45.0% | |
| AAGAAAAAAGTCTCCGGTGG+TGG | + | chr4.4:43068027-43068046 | MS.gene61171:CDS | 45.0% | |
| AGCGAGAATTCCACCGTAAT+CGG | - | chr4.4:43068100-43068119 | None:intergenic | 45.0% | |
| ATCGTCGTCATCATCTTCGT+CGG | + | chr4.4:43068200-43068219 | MS.gene61171:CDS | 45.0% | |
| CATGCAATACGTCGTCGTAA+AGG | + | chr4.4:43068332-43068351 | MS.gene61171:CDS | 45.0% | |
| CGAGTGTTACCGCTAAAAAG+TGG | - | chr4.4:43067124-43067143 | None:intergenic | 45.0% | |
| CGGAAACCAAATCTCGGAAA+CGG | + | chr4.4:43067423-43067442 | MS.gene61171:CDS | 45.0% | |
| GACAGCATTAAGGTAGTGCT+TGG | - | chr4.4:43067155-43067174 | None:intergenic | 45.0% | |
| GCACTACCTTAATGCTGTCA+AGG | + | chr4.4:43067156-43067175 | MS.gene61171:CDS | 45.0% | |
| GCATGGTAAACTGTGGGTAT+GGG | + | chr4.4:43067494-43067513 | MS.gene61171:CDS | 45.0% | |
| GCTGATTGCATGGTAAACTG+TGG | + | chr4.4:43067487-43067506 | MS.gene61171:CDS | 45.0% | |
| GGTTTCTTCTCGGAGAAGAA+TGG | + | chr4.4:43068373-43068392 | MS.gene61171:CDS | 45.0% | |
| TGCATGGTAAACTGTGGGTA+TGG | + | chr4.4:43067493-43067512 | MS.gene61171:CDS | 45.0% | |
| TGTATCCGACCCGAAACTAA+GGG | + | chr4.4:43068587-43068606 | MS.gene61171:CDS | 45.0% | |
| TTCATGACATAGCGTGTGAG+AGG | - | chr4.4:43068060-43068079 | None:intergenic | 45.0% | |
| TTCGACGATCCGTTGAAGAA+CGG | - | chr4.4:43067345-43067364 | None:intergenic | 45.0% | |
| TTGTATCCGACCCGAAACTA+AGG | + | chr4.4:43068586-43068605 | MS.gene61171:CDS | 45.0% | |
| ! | GAACGGTTAGAAACGGATTC+AGG | - | chr4.4:43067328-43067347 | None:intergenic | 45.0% |
| ! | GAGAGTTTCCATGCTTGCTA+TGG | + | chr4.4:43067447-43067466 | MS.gene61171:CDS | 45.0% |
| ! | TCTATGAGAAGAATCGGCTC+GGG | + | chr4.4:43068661-43068680 | MS.gene61171:CDS | 45.0% |
| ! | TTGCATTTTGCCACCATGTC+CGG | - | chr4.4:43067808-43067827 | None:intergenic | 45.0% |
| !! | AAGGTTTTGTCAACGTTGCC+GGG | + | chr4.4:43068459-43068478 | MS.gene61171:CDS | 45.0% |
| !! | AGCGTTGATTGGTTCGAAGT+TGG | + | chr4.4:43068620-43068639 | MS.gene61171:CDS | 45.0% |
| !! | AGCTCTTACAAGAGTGTTGG+AGG | - | chr4.4:43067224-43067243 | None:intergenic | 45.0% |
| !! | AGTTTCCATGCTTGCTATGG+CGG | + | chr4.4:43067450-43067469 | MS.gene61171:CDS | 45.0% |
| !! | CAAGGTTTTGTCAACGTTGC+CGG | + | chr4.4:43068458-43068477 | MS.gene61171:CDS | 45.0% |
| !! | GTTGATTGGTTCGAAGTTGG+TGG | + | chr4.4:43068623-43068642 | MS.gene61171:CDS | 45.0% |
| !! | TCTTACAAGAGTGTTGGAGG+TGG | - | chr4.4:43067221-43067240 | None:intergenic | 45.0% |
| AACGGCTTGTAAACGGACGT+TGG | - | chr4.4:43067929-43067948 | None:intergenic | 50.0% | |
| AAGGCTGTTAAAGTCGCCGT+GGG | + | chr4.4:43067661-43067680 | MS.gene61171:CDS | 50.0% | |
| ATGACGGTGGAGCAGGTTAA+GGG | + | chr4.4:43068504-43068523 | MS.gene61171:CDS | 50.0% | |
| CAAATCCGCCATAGCAAGCA+TGG | - | chr4.4:43067458-43067477 | None:intergenic | 50.0% | |
| CGATAGTACGACTCCGGTAA+CGG | - | chr4.4:43068150-43068169 | None:intergenic | 50.0% | |
| CGTCGTCGGAAACCAAATCT+CGG | + | chr4.4:43067417-43067436 | MS.gene61171:CDS | 50.0% | |
| CTCTCTCCGTTTCCGAGATT+TGG | - | chr4.4:43067432-43067451 | None:intergenic | 50.0% | |
| GATGAATTCTCGCTGCGCAT+CGG | - | chr4.4:43068177-43068196 | None:intergenic | 50.0% | |
| GGACTTCGATAGTACGACTC+CGG | - | chr4.4:43068156-43068175 | None:intergenic | 50.0% | |
| TATGCCGCAATCTCCGTTAC+CGG | + | chr4.4:43068134-43068153 | MS.gene61171:CDS | 50.0% | |
| TCACGGGCGAATAGAGCTAA+GGG | - | chr4.4:43068805-43068824 | None:intergenic | 50.0% | |
| ! | CTCATCCCTTAGTTTCGGGT+CGG | - | chr4.4:43068595-43068614 | None:intergenic | 50.0% |
| ! | GGGTTGTTGGTGGAGATTCA+GGG | + | chr4.4:43068742-43068761 | MS.gene61171:CDS | 50.0% |
| !! | TCGAAGTTGGTGGCGAAGTA+CGG | + | chr4.4:43068633-43068652 | MS.gene61171:CDS | 50.0% |
| CGCAGAGAATTCTTTCGCCG+TGG | - | chr4.4:43067696-43067715 | None:intergenic | 55.0% | |
| GAAGGCTGTTAAAGTCGCCG+TGG | + | chr4.4:43067660-43067679 | MS.gene61171:CDS | 55.0% | |
| GAGAGGATGAACACCACCAC+CGG | - | chr4.4:43068043-43068062 | None:intergenic | 55.0% | |
| TCCACCGTAATCGGCGAGAA+AGG | - | chr4.4:43068091-43068110 | None:intergenic | 55.0% | |
| ! | ACCTTTCTCGCCGATTACGG+TGG | + | chr4.4:43068087-43068106 | MS.gene61171:CDS | 55.0% |
| ! | GATGACGGTGGAGCAGGTTA+AGG | + | chr4.4:43068503-43068522 | MS.gene61171:CDS | 55.0% |
| ! | GCCGTGGGAACACTTTTCCA+CGG | + | chr4.4:43067676-43067695 | MS.gene61171:CDS | 55.0% |
| ! | GGGGTTGTTGGTGGAGATTC+AGG | + | chr4.4:43068741-43068760 | MS.gene61171:CDS | 55.0% |
| !! | AAGTTGGTGGCGAAGTACGG+TGG | + | chr4.4:43068636-43068655 | MS.gene61171:CDS | 55.0% |
| !! | GACTCCGGTAACGGAGATTG+CGG | - | chr4.4:43068141-43068160 | None:intergenic | 55.0% |
| !! | GCCGTGGAAAAGTGTTCCCA+CGG | - | chr4.4:43067680-43067699 | None:intergenic | 55.0% |
| CTCGGCGATTCTCTTCCCTG+CGG | - | chr4.4:43067734-43067753 | None:intergenic | 60.0% | |
| ! | TTCCACGACGGAGATGACGG+TGG | + | chr4.4:43068491-43068510 | MS.gene61171:CDS | 60.0% |
| !! | GAGAAGAATCGGCTCGGGTC+CGG | + | chr4.4:43068666-43068685 | MS.gene61171:CDS | 60.0% |
| CTCCACCGTCATCTCCGTCG+TGG | - | chr4.4:43068496-43068515 | None:intergenic | 65.0% | |
| GGCGATTCTCTTCCCTGCGG+CGG | - | chr4.4:43067731-43067750 | None:intergenic | 65.0% | |
| ! | GACGGAGATGACGGTGGAGC+AGG | + | chr4.4:43068497-43068516 | MS.gene61171:CDS | 65.0% |
| ACGACGTGTTCGCCGCCGCA+GGG | + | chr4.4:43067716-43067735 | MS.gene61171:CDS | 70.0% | |
| GACGACGTGTTCGCCGCCGC+AGG | + | chr4.4:43067715-43067734 | MS.gene61171:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 43067046 | 43068824 | 43067046 | ID=MS.gene61171 |
| chr4.4 | mRNA | 43067046 | 43068824 | 43067046 | ID=MS.gene61171.t1;Parent=MS.gene61171 |
| chr4.4 | exon | 43067046 | 43068824 | 43067046 | ID=MS.gene61171.t1.exon1;Parent=MS.gene61171.t1 |
| chr4.4 | CDS | 43067046 | 43068824 | 43067046 | ID=cds.MS.gene61171.t1;Parent=MS.gene61171.t1 |
| Gene Sequence |
| Protein sequence |
