AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene61376

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 30 sgRNAs with CRISPR-Local

Find 221 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGCATCACAATATTGAATT+TGG 0.165572 6.3:+80439250 None:intergenic
TTTATTTGTCCATTTGATTA+TGG 0.224110 6.3:-80440609 MS.gene61376:CDS
GTAGCACCTTCAACGATAAA+TGG 0.320614 6.3:-80442131 MS.gene61376:CDS
GCAGAGTTCATTGGCATACT+AGG 0.350554 6.3:-80440648 MS.gene61376:intron
CGGTTTCCATTTATCGTTGA+AGG 0.365309 6.3:+80442125 None:intergenic
ATCTAAGTCATGATCATTGT+TGG 0.399875 6.3:-80442555 None:intergenic
TTAATCTTTGCAGAGTTCAT+TGG 0.415750 6.3:-80440657 MS.gene61376:intron
ACAAATAAAACCTTCGGATA+AGG 0.434235 6.3:+80440623 None:intergenic
TCTTCTGAACAAGGCGGAGG+TGG 0.475561 6.3:-80442047 MS.gene61376:CDS
AAATGGACAAATAAAACCTT+CGG 0.493339 6.3:+80440617 None:intergenic
GGTTTGATTCGCATGCCATT+CGG 0.494201 6.3:+80442105 None:intergenic
CTAGCTTGACCATAATCAAA+TGG 0.494548 6.3:+80440600 None:intergenic
CTCCGCCTTGTTCAGAAGAT+CGG 0.504922 6.3:+80442051 None:intergenic
TCTAAGTCATGATCATTGTT+GGG 0.511051 6.3:-80442554 None:intergenic
ATTCTAGATCTAAAAGAAAG+TGG 0.520543 6.3:+80439156 None:intergenic
TCCCTCCTTCTTCTTCACAT+CGG 0.528047 6.3:+80442015 None:intergenic
AGAAGCCGATGTGAAGAAGA+AGG 0.532467 6.3:-80442020 MS.gene61376:CDS
CATTTGATTATGGTCAAGCT+AGG 0.543892 6.3:-80440599 MS.gene61376:intron
CGATCTTCTGAACAAGGCGG+AGG 0.548594 6.3:-80442050 MS.gene61376:CDS
GGGACTGAAGCGGCATGTGA+AGG 0.554999 6.3:-80441996 MS.gene61376:intron
ATACTAGGTTCCTTATCCGA+AGG 0.568215 6.3:-80440633 MS.gene61376:CDS
GCCGATGTGAAGAAGAAGGA+GGG 0.579212 6.3:-80442016 MS.gene61376:CDS
GCTTTCCGATCTTCTGAACA+AGG 0.584830 6.3:-80442056 MS.gene61376:CDS
CTCAAAATTGAATGTGACGT+CGG 0.609907 6.3:-80442083 MS.gene61376:CDS
AACGATAAATGGAAACCGAA+TGG 0.612701 6.3:-80442120 MS.gene61376:CDS
AGCCGATGTGAAGAAGAAGG+AGG 0.642473 6.3:-80442017 MS.gene61376:CDS
AGAAGAAGGAGGGACTGAAG+CGG 0.644736 6.3:-80442006 MS.gene61376:CDS
CGTTGAAGGTGCTACAACTG+TGG 0.652592 6.3:+80442139 None:intergenic
TACAACTGTGGTGATTGTAA+CGG 0.685691 6.3:+80442151 None:intergenic
TTCCGATCTTCTGAACAAGG+CGG 0.700783 6.3:-80442053 MS.gene61376:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAAATTAGTATTGTAA+TGG - chr6.3:80441376-80441395 MS.gene61376:intron 10.0%
!! ATAAAAATTAGTATTGTAAT+GGG - chr6.3:80441377-80441396 MS.gene61376:intron 10.0%
!! AAGAATGACTTATATTAAAA+TGG + chr6.3:80440614-80440633 None:intergenic 15.0%
!! AATTGAAAGATTATGTTTAT+TGG + chr6.3:80439367-80439386 None:intergenic 15.0%
!! ATATTAGAAATAAGTATGAT+TGG + chr6.3:80439235-80439254 None:intergenic 15.0%
!! ATTCAAAATACTACAAAATA+TGG + chr6.3:80441885-80441904 None:intergenic 15.0%
!! TAAAAAAATTGCTTACAATT+AGG - chr6.3:80441546-80441565 MS.gene61376:intron 15.0%
!! TAAAAAGAAAAAAACATTGT+CGG + chr6.3:80442084-80442103 None:intergenic 15.0%
!!! ATATTTTGTAGTATTTTGAA+TGG - chr6.3:80441884-80441903 MS.gene61376:intron 15.0%
!!! ATTTTAATATAAGTCATTCT+TGG - chr6.3:80440613-80440632 MS.gene61376:CDS 15.0%
!!! TATTTTGTAGTATTTTGAAT+GGG - chr6.3:80441885-80441904 MS.gene61376:intron 15.0%
!!! TCATTATAATCCATATTTTT+AGG - chr6.3:80441995-80442014 MS.gene61376:intron 15.0%
!! AAAAAGATGTTCACAAAAAA+AGG - chr6.3:80442489-80442508 MS.gene61376:intron 20.0%
!! AAATTCAATCAGATTCTTTA+AGG + chr6.3:80442314-80442333 None:intergenic 20.0%
!! GAAGATAAATAATCAAAGAA+TGG + chr6.3:80440895-80440914 None:intergenic 20.0%
!! TAGAACTCTTAAATAAGAAA+TGG + chr6.3:80440530-80440549 None:intergenic 20.0%
!! TCCATTGATACAAAATTAAT+TGG + chr6.3:80440425-80440444 None:intergenic 20.0%
!! TTTATTTGAATGAATGACAT+TGG - chr6.3:80441758-80441777 MS.gene61376:intron 20.0%
!! TTTATTTGTCCATTTGATTA+TGG - chr6.3:80441082-80441101 MS.gene61376:intron 20.0%
!!! AATATAAGCATATACCTTTT+GGG + chr6.3:80442022-80442041 None:intergenic 20.0%
!!! ATATAGCATAATGTGTATTT+TGG - chr6.3:80441909-80441928 MS.gene61376:intron 20.0%
!!! ATTTTGTAGTATTTTGAATG+GGG - chr6.3:80441886-80441905 MS.gene61376:intron 20.0%
!!! GACAAAATTCTATATTTTGT+TGG - chr6.3:80441831-80441850 MS.gene61376:intron 20.0%
!!! TCCAATTAATTTTGTATCAA+TGG - chr6.3:80440421-80440440 MS.gene61376:intron 20.0%
!!! TTTTCATTATTTCTTTTTGC+AGG - chr6.3:80442399-80442418 MS.gene61376:intron 20.0%
! AAACAGAAACACAGTAATTT+AGG + chr6.3:80440184-80440203 None:intergenic 25.0%
! AAATGGACAAATAAAACCTT+CGG + chr6.3:80441077-80441096 None:intergenic 25.0%
! AAATTGAATAGTCATCGAAA+CGG + chr6.3:80439943-80439962 None:intergenic 25.0%
! AAGCATCACAATATTGAATT+TGG + chr6.3:80442444-80442463 None:intergenic 25.0%
! ATAATAAACATTGCAAGCTT+AGG - chr6.3:80440504-80440523 MS.gene61376:intron 25.0%
! ATACATGTCAAATATGTGAA+TGG + chr6.3:80440867-80440886 None:intergenic 25.0%
! ATTCTAGATCTAAAAGAAAG+TGG + chr6.3:80442538-80442557 None:intergenic 25.0%
! ATTTGTGGTGAAAACAAATA+GGG - chr6.3:80442158-80442177 MS.gene61376:CDS 25.0%
! GAATTCAAAATTAAATAGCC+AGG + chr6.3:80440019-80440038 None:intergenic 25.0%
! GATAACTATGCATACTATTA+TGG - chr6.3:80441313-80441332 MS.gene61376:intron 25.0%
! GTTTCAAAACAAGTAAGTTT+AGG - chr6.3:80440655-80440674 MS.gene61376:intron 25.0%
! TACATGTCAAATATGTGAAT+GGG + chr6.3:80440866-80440885 None:intergenic 25.0%
! TCACTTTCTTAATGCAATTA+AGG + chr6.3:80440041-80440060 None:intergenic 25.0%
! TTAAAATGATACAGGTTAGT+GGG - chr6.3:80442236-80442255 MS.gene61376:intron 25.0%
! TTAATATAAGTCATTCTTGG+TGG - chr6.3:80440616-80440635 MS.gene61376:CDS 25.0%
! TTATGTTTATTGGAAACAGA+TGG + chr6.3:80439357-80439376 None:intergenic 25.0%
! TTTCAGAAGTTATCAAATTG+TGG + chr6.3:80441281-80441300 None:intergenic 25.0%
! TTTCTTTCCATTACAATAAC+TGG + chr6.3:80442125-80442144 None:intergenic 25.0%
!! AGTGAAAAGATGAACAATTT+TGG + chr6.3:80439907-80439926 None:intergenic 25.0%
!! ATATAAGCATATACCTTTTG+GGG + chr6.3:80442021-80442040 None:intergenic 25.0%
!! CAATATAAGCATATACCTTT+TGG + chr6.3:80442023-80442042 None:intergenic 25.0%
!! GTGAAAAGATGAACAATTTT+GGG + chr6.3:80439906-80439925 None:intergenic 25.0%
!! TAAAAGCATGTTTATACCAA+TGG + chr6.3:80440277-80440296 None:intergenic 25.0%
!! TTATTGTAATGGAAAGAAAC+AGG - chr6.3:80442126-80442145 MS.gene61376:CDS 25.0%
!! TTTAAAATGATACAGGTTAG+TGG - chr6.3:80442235-80442254 MS.gene61376:intron 25.0%
!!! CATTTTCCTGTTTTTGTTTT+TGG - chr6.3:80441125-80441144 MS.gene61376:intron 25.0%
!!! TAATGTGTATTTTGGTTCAT+CGG - chr6.3:80441917-80441936 MS.gene61376:intron 25.0%
!!! TCTGAAATGTGAAAATGTTT+TGG - chr6.3:80441666-80441685 MS.gene61376:intron 25.0%
!!! TTGTGACTTTAAAATGATAC+AGG - chr6.3:80442228-80442247 MS.gene61376:intron 25.0%
AAAACCATTAGAATTAGGGT+TGG + chr6.3:80439282-80439301 None:intergenic 30.0%
AAAATAAAAAAAGGAGCAGC+AGG + chr6.3:80440583-80440602 None:intergenic 30.0%
AACCTGATTTCATATCCTTT+GGG + chr6.3:80441575-80441594 None:intergenic 30.0%
ACAAATAAAACCTTCGGATA+AGG + chr6.3:80441071-80441090 None:intergenic 30.0%
ACATGTCAAATATGTGAATG+GGG + chr6.3:80440865-80440884 None:intergenic 30.0%
ACATTCAAGGAGAAAAATTG+CGG + chr6.3:80441964-80441983 None:intergenic 30.0%
ACGCAATTCAATTCATAGAA+AGG + chr6.3:80439733-80439752 None:intergenic 30.0%
AGCCAAAACCATTAGAATTA+GGG + chr6.3:80439286-80439305 None:intergenic 30.0%
ATGCATCCAAAAACAAAAAC+AGG + chr6.3:80441134-80441153 None:intergenic 30.0%
CATTTGTGGTGAAAACAAAT+AGG - chr6.3:80442157-80442176 MS.gene61376:CDS 30.0%
CGCAATTCAATTCATAGAAA+GGG + chr6.3:80439732-80439751 None:intergenic 30.0%
CTTGAATCAATTATTGATGC+AGG - chr6.3:80440328-80440347 MS.gene61376:intron 30.0%
GAATTGCAGATAAATGTGAA+CGG + chr6.3:80440835-80440854 None:intergenic 30.0%
GCTTGCAATGTTTATTATGA+TGG + chr6.3:80440502-80440521 None:intergenic 30.0%
TAAAATGATACAGGTTAGTG+GGG - chr6.3:80442237-80442256 MS.gene61376:intron 30.0%
TAACCAGATAAATGATGAGA+GGG - chr6.3:80439178-80439197 MS.gene61376:CDS 30.0%
TACTACTAACAGAGAATTGA+AGG + chr6.3:80440706-80440725 None:intergenic 30.0%
TATCAAATTGTGGTTACCTT+TGG + chr6.3:80441271-80441290 None:intergenic 30.0%
TGAACTATTGGGAAATCATA+TGG - chr6.3:80441711-80441730 MS.gene61376:intron 30.0%
TGTTGAATCTGTGTTAGTAA+TGG - chr6.3:80440301-80440320 MS.gene61376:intron 30.0%
TTAATCTTTGCAGAGTTCAT+TGG - chr6.3:80441034-80441053 MS.gene61376:intron 30.0%
TTCCATGTTGATATATGAAG+CGG + chr6.3:80440086-80440105 None:intergenic 30.0%
TTCTTTATTGCCTTGCTTTA+TGG + chr6.3:80440454-80440473 None:intergenic 30.0%
! AAAGAATGATTGACTCCATA+AGG + chr6.3:80439801-80439820 None:intergenic 30.0%
! AACCCTAATTCTAATGGTTT+TGG - chr6.3:80439281-80439300 MS.gene61376:intron 30.0%
! AAGAATGATTGACTCCATAA+GGG + chr6.3:80439800-80439819 None:intergenic 30.0%
! ATTTCTTTTTGCAGGTTATG+TGG - chr6.3:80442407-80442426 MS.gene61376:intron 30.0%
! CGACTCAGATTTTCAAAAAT+CGG - chr6.3:80439858-80439877 MS.gene61376:intron 30.0%
! GAAATCAGGTTGTCAAAAAA+TGG - chr6.3:80441584-80441603 MS.gene61376:intron 30.0%
! GGGGATTAAAATAGTCTTTT+TGG - chr6.3:80442257-80442276 MS.gene61376:intron 30.0%
!! ATTCAACTGAGAGTATTCAT+AGG - chr6.3:80442201-80442220 MS.gene61376:intron 30.0%
!! ATTTTGTTGGCTGAACTATT+GGG - chr6.3:80441844-80441863 MS.gene61376:intron 30.0%
!! GAAATGTGAAAATGTTTTGG+CGG - chr6.3:80441669-80441688 MS.gene61376:intron 30.0%
!! TATTTTGTTGGCTGAACTAT+TGG - chr6.3:80441843-80441862 MS.gene61376:intron 30.0%
!! TTCAACTGAGAGTATTCATA+GGG - chr6.3:80442202-80442221 MS.gene61376:intron 30.0%
!!! GCAATTTTTTTATCTCATGC+AGG + chr6.3:80441538-80441557 None:intergenic 30.0%
AAAATGATACAGGTTAGTGG+GGG - chr6.3:80442238-80442257 MS.gene61376:intron 35.0%
AACCGCTTCATATATCAACA+TGG - chr6.3:80440081-80440100 MS.gene61376:intron 35.0%
AACCTTTCTGATCCTATAGT+TGG + chr6.3:80440681-80440700 None:intergenic 35.0%
AACGATAAATGGAAACCGAA+TGG - chr6.3:80439571-80439590 MS.gene61376:intron 35.0%
AACTATAGGATCAGAAAGGT+TGG - chr6.3:80440680-80440699 MS.gene61376:intron 35.0%
AAGCCCTATAAAATACCATC+AGG - chr6.3:80441627-80441646 MS.gene61376:intron 35.0%
ACCTTTGGTCATTATTCAGT+AGG + chr6.3:80441256-80441275 None:intergenic 35.0%
AGTAAGTTTAGGCCAACTAT+AGG - chr6.3:80440666-80440685 MS.gene61376:intron 35.0%
ATCAACACAGATGCATCTAA+CGG + chr6.3:80439323-80439342 None:intergenic 35.0%
ATCAGGATTGTAGGAATCTA+TGG - chr6.3:80441416-80441435 MS.gene61376:intron 35.0%
ATCTAACGGTCATAACAGTT+TGG + chr6.3:80439309-80439328 None:intergenic 35.0%
CAACCTGATTTCATATCCTT+TGG + chr6.3:80441576-80441595 None:intergenic 35.0%
CAACGTTCCAGTTATTGTAA+TGG - chr6.3:80442115-80442134 MS.gene61376:CDS 35.0%
CATATATGAATCCATCCAAG+AGG - chr6.3:80441490-80441509 MS.gene61376:intron 35.0%
CATTTGATTATGGTCAAGCT+AGG - chr6.3:80441092-80441111 MS.gene61376:intron 35.0%
CTAACCAGATAAATGATGAG+AGG - chr6.3:80439177-80439196 MS.gene61376:CDS 35.0%
CTAGCTTGACCATAATCAAA+TGG + chr6.3:80441094-80441113 None:intergenic 35.0%
CTCAAAATTGAATGTGACGT+CGG - chr6.3:80439608-80439627 MS.gene61376:intron 35.0%
CTCTGCAAAGATTAACGAAA+AGG + chr6.3:80441029-80441048 None:intergenic 35.0%
GAGCCAAAACCATTAGAATT+AGG + chr6.3:80439287-80439306 None:intergenic 35.0%
GTTGACTGATAACAATCCAT+TGG - chr6.3:80440258-80440277 MS.gene61376:intron 35.0%
TAAGGCCAACAAATAAGCTA+AGG - chr6.3:80440736-80440755 MS.gene61376:intron 35.0%
TACAACTGTGGTGATTGTAA+CGG + chr6.3:80439543-80439562 None:intergenic 35.0%
TCCTACTGAATAATGACCAA+AGG - chr6.3:80441252-80441271 MS.gene61376:intron 35.0%
TCGATGACTATTCAATTTCG+TGG - chr6.3:80439945-80439964 MS.gene61376:intron 35.0%
TCTGTTAGTAGTATCTCGAT+CGG - chr6.3:80440713-80440732 MS.gene61376:intron 35.0%
TGGAGAATTTCCATAAAGCA+AGG - chr6.3:80440441-80440460 MS.gene61376:intron 35.0%
TTTCCCTCTCATCATTTATC+TGG + chr6.3:80439184-80439203 None:intergenic 35.0%
TTTGGCGGTAATTTGAGAAT+TGG - chr6.3:80441684-80441703 MS.gene61376:intron 35.0%
! AGGGAAAACCACTTTGAAAA+GGG - chr6.3:80439197-80439216 MS.gene61376:CDS 35.0%
! AGTATTGTAATGGGAATGTG+TGG - chr6.3:80441386-80441405 MS.gene61376:intron 35.0%
! ATAAGTATGATTGGCTAGTC+CGG + chr6.3:80439226-80439245 None:intergenic 35.0%
! ATTGAGCATCGCTGTATATA+GGG - chr6.3:80441159-80441178 MS.gene61376:intron 35.0%
! ATTTCAAGTCACAAGTGCAT+TGG - chr6.3:80439384-80439403 MS.gene61376:intron 35.0%
! CTATTCAATTTCGTGGATTG+AGG - chr6.3:80439952-80439971 MS.gene61376:intron 35.0%
! CTCAGATTTTCAAAAATCGG+AGG - chr6.3:80439861-80439880 MS.gene61376:intron 35.0%
! CTCATGCAGGATTTTATCTT+TGG + chr6.3:80441525-80441544 None:intergenic 35.0%
! CTCTTGGATGGATTCATATA+TGG + chr6.3:80441492-80441511 None:intergenic 35.0%
! GAGCCTGATGGTATTTTATA+GGG + chr6.3:80441633-80441652 None:intergenic 35.0%
! TGAGCCTGATGGTATTTTAT+AGG + chr6.3:80441634-80441653 None:intergenic 35.0%
! TGTTTCTGTTTATCTCAGCT+CGG - chr6.3:80440193-80440212 MS.gene61376:intron 35.0%
!!! TGTTACATCAGTTTTAGACC+TGG - chr6.3:80439998-80440017 MS.gene61376:intron 35.0%
AAAGTGGAGCGTGAGTTTAT+TGG + chr6.3:80439431-80439450 None:intergenic 40.0%
AACAGGATCTGCTACATTTG+TGG - chr6.3:80442143-80442162 MS.gene61376:CDS 40.0%
AAGGCAGTTGCTTCTTTCAT+TGG + chr6.3:80442295-80442314 None:intergenic 40.0%
AATAGGAACACCATCATCGA+CGG + chr6.3:80440762-80440781 None:intergenic 40.0%
AATATGTGAATGGGGTGCAA+AGG + chr6.3:80440857-80440876 None:intergenic 40.0%
AGGACGATTGATAGGCAAAT+AGG + chr6.3:80440779-80440798 None:intergenic 40.0%
ATACTAGGTTCCTTATCCGA+AGG - chr6.3:80441058-80441077 MS.gene61376:intron 40.0%
CGGTTTCCATTTATCGTTGA+AGG + chr6.3:80439569-80439588 None:intergenic 40.0%
GAACTCGCATAACACATTCA+AGG + chr6.3:80441977-80441996 None:intergenic 40.0%
GACACTTAAGGACGATTGAT+AGG + chr6.3:80440787-80440806 None:intergenic 40.0%
GAGGTAAGAATGGAATGGAA+TGG - chr6.3:80439971-80439990 MS.gene61376:intron 40.0%
GGATTGAGGTAAGAATGGAA+TGG - chr6.3:80439966-80439985 MS.gene61376:intron 40.0%
GGTGGGAGAATATTGAAAAG+TGG + chr6.3:80439447-80439466 None:intergenic 40.0%
GTAGCACCTTCAACGATAAA+TGG - chr6.3:80439560-80439579 MS.gene61376:intron 40.0%
GTTGGGTAACTCATCATAGT+TGG - chr6.3:80439154-80439173 MS.gene61376:CDS 40.0%
TAGTAGTATCTCGATCGGTA+AGG - chr6.3:80440718-80440737 MS.gene61376:intron 40.0%
TATGAATCCATCCAAGAGGA+AGG - chr6.3:80441494-80441513 MS.gene61376:intron 40.0%
TATGGTCAAGCTAGGTAAGT+TGG - chr6.3:80441100-80441119 MS.gene61376:intron 40.0%
TCTTAGCTGCAAGACACTTA+AGG + chr6.3:80440799-80440818 None:intergenic 40.0%
TGCCCAAAGGATATGAAATC+AGG - chr6.3:80441570-80441589 MS.gene61376:intron 40.0%
TGTGCCAACCCTAATTCTAA+TGG - chr6.3:80439275-80439294 MS.gene61376:intron 40.0%
TTGACTCCATAAGGGTTCTA+AGG + chr6.3:80439792-80439811 None:intergenic 40.0%
TTGTAGGAATCTATGGCACA+TGG - chr6.3:80441423-80441442 MS.gene61376:intron 40.0%
! AGAATTGGTGGCTGAACTAT+TGG - chr6.3:80441699-80441718 MS.gene61376:intron 40.0%
! CATTGAGCATCGCTGTATAT+AGG - chr6.3:80441158-80441177 MS.gene61376:intron 40.0%
! CGACGTCACATTCAATTTTG+AGG + chr6.3:80439610-80439629 None:intergenic 40.0%
! CTTACAATTAGGTTGCCCAA+AGG - chr6.3:80441557-80441576 MS.gene61376:intron 40.0%
! GAATTGGTGGCTGAACTATT+GGG - chr6.3:80441700-80441719 MS.gene61376:intron 40.0%
! GAGGGAAAACCACTTTGAAA+AGG - chr6.3:80439196-80439215 MS.gene61376:CDS 40.0%
! TGACTCCATAAGGGTTCTAA+GGG + chr6.3:80439791-80439810 None:intergenic 40.0%
! TTCGTGGATTGAGGTAAGAA+TGG - chr6.3:80439961-80439980 MS.gene61376:intron 40.0%
!! AATCGGAGGTGTTGAATGTT+CGG - chr6.3:80439875-80439894 MS.gene61376:intron 40.0%
!! ACAGTTCTTAAACTTGCTGC+AGG + chr6.3:80440556-80440575 None:intergenic 40.0%
!! TTGGAAACAGATGGATTGTG+TGG + chr6.3:80439348-80439367 None:intergenic 40.0%
!!! CCATATTTTTAGGCCCCAAA+AGG - chr6.3:80442005-80442024 MS.gene61376:CDS 40.0%
!!! CCTTTTGGGGCCTAAAAATA+TGG + chr6.3:80442008-80442027 None:intergenic 40.0%
AAGAGGAAGCTGACTATGTG+GGG - chr6.3:80440224-80440243 MS.gene61376:intron 45.0%
AATGGAGAGAGTTCCAGAGA+CGG + chr6.3:80439474-80439493 None:intergenic 45.0%
AGAAGCCGATGTGAAGAAGA+AGG - chr6.3:80439671-80439690 MS.gene61376:intron 45.0%
AGTCATCGAAACGGAGAAGA+AGG + chr6.3:80439934-80439953 None:intergenic 45.0%
CTTGCAGCTAAGATAACCGT+CGG - chr6.3:80440806-80440825 MS.gene61376:intron 45.0%
CTTTGGATTCTCCTTCCTCT+TGG + chr6.3:80441508-80441527 None:intergenic 45.0%
GAAGAGGAAGCTGACTATGT+GGG - chr6.3:80440223-80440242 MS.gene61376:intron 45.0%
GAATGTGTGGAGACTACATC+AGG - chr6.3:80441399-80441418 MS.gene61376:intron 45.0%
GACGGCCTTAGCTTATTTGT+TGG + chr6.3:80440744-80440763 None:intergenic 45.0%
GCAGAGTTCATTGGCATACT+AGG - chr6.3:80441043-80441062 MS.gene61376:intron 45.0%
GCTTTCCGATCTTCTGAACA+AGG - chr6.3:80439635-80439654 MS.gene61376:intron 45.0%
GGAGACTACATCAGGATTGT+AGG - chr6.3:80441407-80441426 MS.gene61376:intron 45.0%
GGCCAACTATAGGATCAGAA+AGG - chr6.3:80440676-80440695 MS.gene61376:intron 45.0%
GGCGGTAATTTGAGAATTGG+TGG - chr6.3:80441687-80441706 MS.gene61376:intron 45.0%
TAAATGTGAACGGAGACCGA+CGG + chr6.3:80440825-80440844 None:intergenic 45.0%
TACCATCAGGCTCATAGCTT+TGG - chr6.3:80441640-80441659 MS.gene61376:intron 45.0%
TCAGCTCGGATATGATGAAG+AGG - chr6.3:80440207-80440226 MS.gene61376:intron 45.0%
TCATCCCCTTAGAACCCTTA+TGG - chr6.3:80439783-80439802 MS.gene61376:intron 45.0%
TCCCTCCTTCTTCTTCACAT+CGG + chr6.3:80439679-80439698 None:intergenic 45.0%
TGAAGAGGAAGCTGACTATG+TGG - chr6.3:80440222-80440241 MS.gene61376:intron 45.0%
TTCCGATCTTCTGAACAAGG+CGG - chr6.3:80439638-80439657 MS.gene61376:intron 45.0%
! ACCACTTTGAAAAGGGTGAC+CGG - chr6.3:80439204-80439223 MS.gene61376:CDS 45.0%
! GGTTTGATTCGCATGCCATT+CGG + chr6.3:80439589-80439608 None:intergenic 45.0%
!! ATCGGAGGTGTTGAATGTTC+GGG - chr6.3:80439876-80439895 MS.gene61376:intron 45.0%
!! GACTCCATAAGGGTTCTAAG+GGG + chr6.3:80439790-80439809 None:intergenic 45.0%
!! GATAAAAATAAAATAAAAAA+AGG + chr6.3:80440592-80440611 None:intergenic 5.0%
AACGGAAAAGCCACAGCTGA+CGG + chr6.3:80439525-80439544 None:intergenic 50.0%
AGAAGAAGGAGGGACTGAAG+CGG - chr6.3:80439685-80439704 MS.gene61376:intron 50.0%
AGAGGAAGCTGACTATGTGG+GGG - chr6.3:80440225-80440244 MS.gene61376:intron 50.0%
AGCCGATGTGAAGAAGAAGG+AGG - chr6.3:80439674-80439693 MS.gene61376:intron 50.0%
AGTCGTCTCAAGCGTTAGAG+AGG + chr6.3:80439843-80439862 None:intergenic 50.0%
GCCGATGTGAAGAAGAAGGA+GGG - chr6.3:80439675-80439694 MS.gene61376:intron 50.0%
GGATTCTCCTTCCTCTTGGA+TGG + chr6.3:80441504-80441523 None:intergenic 50.0%
GTCGTCTCAAGCGTTAGAGA+GGG + chr6.3:80439842-80439861 None:intergenic 50.0%
GTGGAGGATGAGATGACAGA+GGG + chr6.3:80439501-80439520 None:intergenic 50.0%
TAAGCTAAGGCCGTCGATGA+TGG - chr6.3:80440749-80440768 MS.gene61376:intron 50.0%
TGCCAAAGCTATGAGCCTGA+TGG + chr6.3:80441645-80441664 None:intergenic 50.0%
! CTCCGCCTTGTTCAGAAGAT+CGG + chr6.3:80439643-80439662 None:intergenic 50.0%
! TCCGGTCACCCTTTTCAAAG+TGG + chr6.3:80439208-80439227 None:intergenic 50.0%
!! CGTTGAAGGTGCTACAACTG+TGG + chr6.3:80439555-80439574 None:intergenic 50.0%
CGATCTTCTGAACAAGGCGG+AGG - chr6.3:80439641-80439660 MS.gene61376:intron 55.0%
CGTGGAGGATGAGATGACAG+AGG + chr6.3:80439502-80439521 None:intergenic 55.0%
GAGAGTTCCAGAGACGGTAG+TGG + chr6.3:80439468-80439487 None:intergenic 55.0%
GAGATGACAGAGGGAGGCAA+TGG + chr6.3:80439492-80439511 None:intergenic 55.0%
GAGGATGAGATGACAGAGGG+AGG + chr6.3:80439498-80439517 None:intergenic 55.0%
TCTTCTGAACAAGGCGGAGG+TGG - chr6.3:80439644-80439663 MS.gene61376:intron 55.0%
! AGTTCCAGAGACGGTAGTGG+TGG + chr6.3:80439465-80439484 None:intergenic 55.0%
! GTTCCAGAGACGGTAGTGGT+GGG + chr6.3:80439464-80439483 None:intergenic 55.0%
AAAAGCCACAGCTGACGGCG+TGG + chr6.3:80439520-80439539 None:intergenic 60.0%
GGGACTGAAGCGGCATGTGA+AGG - chr6.3:80439695-80439714 MS.gene61376:intron 60.0%
TCTCCCACCACTACCGTCTC+TGG - chr6.3:80439458-80439477 MS.gene61376:intron 60.0%
ATCCTCCACGCCGTCAGCTG+TGG - chr6.3:80439512-80439531 MS.gene61376:intron 65.0%
AGCCACAGCTGACGGCGTGG+AGG + chr6.3:80439517-80439536 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 80439145 80442568 80439145 ID=MS.gene61376
chr6.3 mRNA 80439145 80442568 80439145 ID=MS.gene61376.t1;Parent=MS.gene61376
chr6.3 exon 80442555 80442568 80442555 ID=MS.gene61376.t1.exon1;Parent=MS.gene61376.t1
chr6.3 CDS 80442555 80442568 80442555 ID=cds.MS.gene61376.t1;Parent=MS.gene61376.t1
chr6.3 exon 80441997 80442184 80441997 ID=MS.gene61376.t1.exon2;Parent=MS.gene61376.t1
chr6.3 CDS 80441997 80442184 80441997 ID=cds.MS.gene61376.t1;Parent=MS.gene61376.t1
chr6.3 exon 80440600 80440666 80440600 ID=MS.gene61376.t1.exon3;Parent=MS.gene61376.t1
chr6.3 CDS 80440600 80440666 80440600 ID=cds.MS.gene61376.t1;Parent=MS.gene61376.t1
chr6.3 exon 80439145 80439292 80439145 ID=MS.gene61376.t1.exon4;Parent=MS.gene61376.t1
chr6.3 CDS 80439145 80439292 80439145 ID=cds.MS.gene61376.t1;Parent=MS.gene61376.t1
Gene Sequence

>MS.gene61376

ATGATCATTGTTGGGTAACTCATCATAGTTGGCTAACCAGATAAATGATGAGAGGGAAAACCACTTTGAAAAGGGTGACCGGACTAGCCAATCATACTTATTTCTAATATGAATAACATTGCTAACAAAGTGTGCCAACCCTAATTCTAATGGTTTTGGCTCCAAACTGTTATGACCGTTAGATGCATCTGTGTTGATCTCCACACAATCCATCTGTTTCCAATAAACATAATCTTTCAATTTCAAGTCACAAGTGCATTGGTAACATACACACGTTTCTAATCCAATAAACTCACGCTCCACTTTTCAATATTCTCCCACCACTACCGTCTCTGGAACTCTCTCCATTGCCTCCCTCTGTCATCTCATCCTCCACGCCGTCAGCTGTGGCTTTTCCGTTACAATCACCACAGTTGTAGCACCTTCAACGATAAATGGAAACCGAATGGCATGCGAATCAAACCTCAAAATTGAATGTGACGTCGGAGAAGCTTTCCGATCTTCTGAACAAGGCGGAGGTGGAGATAGAAGCCGATGTGAAGAAGAAGGAGGGACTGAAGCGGCATGTGAAGGTAATCGTTTTACCCTTTCTATGAATTGAATTGCGTTTCTCTACATTTCAGTTTTCAGTTTCACTTTCATCCCCTTAGAACCCTTATGGAGTCAATCATTCTTTACTATTCTCAGTTTATTTCCCTCTCTAACGCTTGAGACGACTCAGATTTTCAAAAATCGGAGGTGTTGAATGTTCGGGTATTCCCAAAATTGTTCATCTTTTCACTTTCACCTTCTTCTCCGTTTCGATGACTATTCAATTTCGTGGATTGAGGTAAGAATGGAATGGAATGGAACGTGTTACATCAGTTTTAGACCTGGCTATTTAATTTTGAATTCCTTAATTGCATTAAGAAAGTGATTGCTGATGATGATATCATCAACCGCTTCATATATCAACATGGAATCGATGTAATATAAAATTTAGATGTCAATCAGATTATGTTTAATTCGTATAATGACAACTATGTTATATTACATACCTAAATTACTGTGTTTCTGTTTATCTCAGCTCGGATATGATGAAGAGGAAGCTGACTATGTGGGGGATGTTTCTAAGTTGACTGATAACAATCCATTGGTATAAACATGCTTTTATAATTGTTGAATCTGTGTTAGTAATGGTGTTCTTGAATCAATTATTGATGCAGGCAAACTCTTATTACTTAGCTTCATTTTATATTTAACTCTAATGATCAATGTCTATGACAGAGTCTTAGTTTCCAATTAATTTTGTATCAATGGAGAATTTCCATAAAGCAAGGCAATAAAGAAAAATCATTTAAGATTATACTTTTAACCATCATAATAAACATTGCAAGCTTAGGCCATTTCTTATTTAAGAGTTCTACTGCCTGCAGCAAGTTTAAGAACTGTACTGCCTGCTGCTCCTTTTTTTATTTTATTTTTATCCATTTTAATATAAGTCATTCTTGGTGGACTCATTTTCTGCTCTGTTTCAAAACAAGTAAGTTTAGGCCAACTATAGGATCAGAAAGGTTGGCCTTCAATTCTCTGTTAGTAGTATCTCGATCGGTAAGGCCAACAAATAAGCTAAGGCCGTCGATGATGGTGTTCCTATTTGCCTATCAATCGTCCTTAAGTGTCTTGCAGCTAAGATAACCGTCGGTCTCCGTTCACATTTATCTGCAATTCCTTTGCACCCCATTCACATATTTGACATGTATGTGTACCATTCTTTGATTATTTATCTTCACTTCATAGTCAAATTTGCACATGCTAATTAGAAAGTTTAAGTTATGTACATTATTATTTTGTTTATAATCGCATAAGCATGATATATGTAATCTGATTGACTACTGAGTTCCTTTTCGTTAATCTTTGCAGAGTTCATTGGCATACTAGGTTCCTTATCCGAAGGTTTTATTTGTCCATTTGATTATGGTCAAGCTAGGTAAGTTGGTTCATTTTCCTGTTTTTGTTTTTGGATGCATTTAGCATTGAGCATCGCTGTATATAGGGTGATTGAACAAGATGTATTGTTTTTTGATTCTTAGCATTACATGTGAATGTGATAAAATGCTACTTCTATTCCTACTGAATAATGACCAAAGGTAACCACAATTTGATAACTTCTGAAATTATAATGACATGATAACTATGCATACTATTATGGCTGAAAGTTAGTGACATGTGCGTTCTAACTTATTTCTTTTAATAAAAATTAGTATTGTAATGGGAATGTGTGGAGACTACATCAGGATTGTAGGAATCTATGGCACATGGCTGCTGATATATTTACAGCTTATACAAAAAATGCATCAAAACTCCATATATGAATCCATCCAAGAGGAAGGAGAATCCAAAGATAAAATCCTGCATGAGATAAAAAAATTGCTTACAATTAGGTTGCCCAAAGGATATGAAATCAGGTTGTCAAAAAATGGCTATACTTTTATATTCTAACAAGCCCTATAAAATACCATCAGGCTCATAGCTTTGGCAGTCTGAAATGTGAAAATGTTTTGGCGGTAATTTGAGAATTGGTGGCTGAACTATTGGGAAATCATATGGTCTTCAAACTATTGTCTTCTAATTTTTATTTGAATGAATGACATTGGTGTCATTAGTTTTGCTTTTGTATGTTATGAGCTAAATCTCTCTAACACTTGACAAAATTCTATATTTTGTTGGCTGAACTATTGGGTGATTAAATGTTATTCCATATTTTGTAGTATTTTGAATGGGGATATAGCATAATGTGTATTTTGGTTCATCGGCTTTGATTGATTTAGAGTAAACCGCAATTTTTCTCCTTGAATGTGTTATGCGAGTTCATTATAATCCATATTTTTAGGCCCCAAAAGGTATATGCTTATATTGTTCTGATCATATGTTTCACAGTGTTTACATTGATGCTACCGACAATGTTTTTTTCTTTTTAATATTCAGATTCAACGTTCCAGTTATTGTAATGGAAAGAAACAGGATCTGCTACATTTGTGGTGAAAACAAATAGGGAAGAAACTTAGATTATATTGATTCAACTGAGAGTATTCATAGGGATTTTGTGACTTTAAAATGATACAGGTTAGTGGGGGATTAAAATAGTCTTTTTGGAGTATGAAGAAACCAATGAAAGAAGCAACTGCCTTAAAGAATCTGATTGAATTTATGCAAAATCACAGTTATGTGTCTTTTGACAATTCTATGACATATCTATTGATCTCATATAAGCATTTTCATTATTTCTTTTTGCAGGTTATGTGGTTATGCAGTAGCCAAATTCAATATTGTGATGCTTGAAAGAAGAATGATTATGCAGCTTGAAAAAGATGTTCACAAAAAAAGGAGAACTCACATGCTGTCGTGCTGCCACTTTCTTTTAGATCTAGAATATAACCAGTAA
Protein sequence

>MS.gene61376.t1

MIIVGCGFSVTITTVVAPSTINGNRMACESNLKIECDVGEAFRSSEQGGGGDRSRCEEEGGTEAACEEFIGILGSLSEGFICPFDYGQARLCGYAVAKFNIVMLERRMIMQLEKDVHKKRRTHMLSCCHFLLDLEYNQ