Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61414.t1 | RHN52877.1 | 96.8 | 63 | 2 | 0 | 1 | 63 | 1 | 63 | 1.60E-24 | 121.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61414.t1 | Q8L9T8 | 54.7 | 53 | 22 | 1 | 11 | 63 | 13 | 63 | 3.0e-08 | 58.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61414.t1 | A0A396HPT2 | 96.8 | 63 | 2 | 0 | 1 | 63 | 1 | 63 | 1.1e-24 | 121.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05527 | MS.gene61414 | 0.98119 | 5.46E-152 | -1.69E-46 |
| MS.gene057432 | MS.gene61414 | 0.803655 | 3.14E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61414.t1 | MTR_6g086365 | 82.540 | 63 | 11 | 0 | 1 | 63 | 1 | 63 | 8.21e-33 | 108 |
| MS.gene61414.t1 | MTR_6g086350 | 82.540 | 63 | 11 | 0 | 1 | 63 | 1 | 63 | 2.88e-31 | 103 |
| MS.gene61414.t1 | MTR_6g086390 | 76.190 | 63 | 15 | 0 | 1 | 63 | 2 | 64 | 2.81e-30 | 100 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61414.t1 | AT3G61640 | 47.458 | 59 | 29 | 1 | 5 | 63 | 6 | 62 | 2.04e-12 | 56.2 |
| MS.gene61414.t1 | AT2G46330 | 50.909 | 55 | 25 | 1 | 9 | 63 | 10 | 62 | 2.42e-12 | 56.2 |
| MS.gene61414.t1 | AT5G53250 | 48.214 | 56 | 24 | 1 | 8 | 63 | 13 | 63 | 2.59e-11 | 53.1 |
Find 12 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACTAGTGATGCTCAGATTC+TGG | 0.273706 | 6.3:-79811804 | MS.gene61414:CDS |
| TTCTCTTATGTTGGTGGCGC+TGG | 0.343746 | 6.3:-79811765 | MS.gene61414:CDS |
| TGCTTATTCTCTTATGTTGG+TGG | 0.427507 | 6.3:-79811771 | MS.gene61414:CDS |
| GTTCTTGGCAATTTCTCACA+TGG | 0.459379 | 6.3:-79811867 | MS.gene61414:CDS |
| AAGAACAAAATAGTAAGAAG+TGG | 0.472674 | 6.3:+79811884 | None:intergenic |
| AATTGCTTATTCTCTTATGT+TGG | 0.478265 | 6.3:-79811774 | MS.gene61414:CDS |
| AGAATCTGAGCATCACTAGT+AGG | 0.516211 | 6.3:+79811806 | None:intergenic |
| TTCTTGGCAATTTCTCACAT+GGG | 0.525548 | 6.3:-79811866 | MS.gene61414:CDS |
| TCACTAGTAGGAGTAGGTGC+TGG | 0.561134 | 6.3:+79811818 | None:intergenic |
| AGAACAAAATAGTAAGAAGT+GGG | 0.592067 | 6.3:+79811885 | None:intergenic |
| GATGCTCAGATTCTGGATCA+AGG | 0.592593 | 6.3:-79811797 | MS.gene61414:CDS |
| TGAGCATCACTAGTAGGAGT+AGG | 0.610713 | 6.3:+79811812 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAGAACAAAATAGTAAGAAG+TGG | + | chr6.3:79811772-79811791 | None:intergenic | 25.0% |
| ! | AATTGCTTATTCTCTTATGT+TGG | - | chr6.3:79811879-79811898 | MS.gene61414:CDS | 25.0% |
| ! | AGAACAAAATAGTAAGAAGT+GGG | + | chr6.3:79811771-79811790 | None:intergenic | 25.0% |
| !! | ACTTCTTACTATTTTGTTCT+TGG | - | chr6.3:79811771-79811790 | MS.gene61414:CDS | 25.0% |
| TGCTTATTCTCTTATGTTGG+TGG | - | chr6.3:79811882-79811901 | MS.gene61414:CDS | 35.0% | |
| TTCTTGGCAATTTCTCACAT+GGG | - | chr6.3:79811787-79811806 | MS.gene61414:CDS | 35.0% | |
| AGAATCTGAGCATCACTAGT+AGG | + | chr6.3:79811850-79811869 | None:intergenic | 40.0% | |
| GTTCTTGGCAATTTCTCACA+TGG | - | chr6.3:79811786-79811805 | MS.gene61414:CDS | 40.0% | |
| TACTAGTGATGCTCAGATTC+TGG | - | chr6.3:79811849-79811868 | MS.gene61414:CDS | 40.0% | |
| GATGCTCAGATTCTGGATCA+AGG | - | chr6.3:79811856-79811875 | MS.gene61414:CDS | 45.0% | |
| TGAGCATCACTAGTAGGAGT+AGG | + | chr6.3:79811844-79811863 | None:intergenic | 45.0% | |
| TCACTAGTAGGAGTAGGTGC+TGG | + | chr6.3:79811838-79811857 | None:intergenic | 50.0% | |
| !! | TTCTCTTATGTTGGTGGCGC+TGG | - | chr6.3:79811888-79811907 | MS.gene61414:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 79811742 | 79811933 | 79811742 | ID=MS.gene61414 |
| chr6.3 | mRNA | 79811742 | 79811933 | 79811742 | ID=MS.gene61414.t1;Parent=MS.gene61414 |
| chr6.3 | exon | 79811742 | 79811933 | 79811742 | ID=MS.gene61414.t1.exon1;Parent=MS.gene61414.t1 |
| chr6.3 | CDS | 79811742 | 79811933 | 79811742 | ID=cds.MS.gene61414.t1;Parent=MS.gene61414.t1 |
| Gene Sequence |
| Protein sequence |
