Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05527.t1 | RHN52877.1 | 98.2 | 56 | 1 | 0 | 8 | 63 | 1 | 56 | 3.70E-21 | 110.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05527.t1 | A0A396HPT2 | 98.2 | 56 | 1 | 0 | 8 | 63 | 1 | 56 | 2.6e-21 | 110.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05527 | MS.gene61414 | 0.98119 | 5.46E-152 | -1.69E-46 |
| MS.gene05527 | MS.gene82042 | 0.822373 | 2.45E-53 | -1.69E-46 |
| MS.gene05527 | MS.gene83618 | 0.804346 | 2.26E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05527.t1 | MTR_6g086365 | 91.071 | 56 | 5 | 0 | 8 | 63 | 1 | 56 | 6.60e-32 | 105 |
| MS.gene05527.t1 | MTR_6g086350 | 80.357 | 56 | 11 | 0 | 8 | 63 | 1 | 56 | 8.70e-26 | 89.7 |
| MS.gene05527.t1 | MTR_6g086390 | 73.684 | 57 | 15 | 0 | 7 | 63 | 1 | 57 | 2.13e-25 | 88.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 12 sgRNAs with CRISPR-Local
Find 14 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACTAGTGATGCTCAGATTC+TGG | 0.273706 | 6.2:-84652813 | MS.gene05527:CDS |
| TTCTCTTATGTTGGTGGCGC+TGG | 0.343746 | 6.2:-84652774 | None:intergenic |
| TGCTTATTCTCTTATGTTGG+TGG | 0.427507 | 6.2:-84652780 | MS.gene05527:CDS |
| GTTCTTGGCAATTTCTCACA+TGG | 0.459379 | 6.2:-84652876 | MS.gene05527:CDS |
| AAGAACAAAATAGTAAGAAG+TGG | 0.472674 | 6.2:+84652893 | None:intergenic |
| AATTGCTTATTCTCTTATGT+TGG | 0.478265 | 6.2:-84652783 | MS.gene05527:CDS |
| AGAATCTGAGCATCACTAGT+AGG | 0.516211 | 6.2:+84652815 | None:intergenic |
| TTCTTGGCAATTTCTCACAT+GGG | 0.525548 | 6.2:-84652875 | MS.gene05527:CDS |
| TCACTAGTAGGAGTAGGTGC+TGG | 0.561134 | 6.2:+84652827 | None:intergenic |
| AGAACAAAATAGTAAGAAGT+GGG | 0.592067 | 6.2:+84652894 | None:intergenic |
| GATGCTCAGATTCTGGATCA+AGG | 0.592593 | 6.2:-84652806 | MS.gene05527:CDS |
| TGAGCATCACTAGTAGGAGT+AGG | 0.610713 | 6.2:+84652821 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTGTCTTCATTATTTTCTT+GGG | + | chr6.2:84652787-84652806 | None:intergenic | 20.0% |
| ! | AAGAACAAAATAGTAAGAAG+TGG | + | chr6.2:84652826-84652845 | None:intergenic | 25.0% |
| ! | AATTGCTTATTCTCTTATGT+TGG | - | chr6.2:84652933-84652952 | MS.gene05527:CDS | 25.0% |
| ! | AGAACAAAATAGTAAGAAGT+GGG | + | chr6.2:84652825-84652844 | None:intergenic | 25.0% |
| !! | ACTTCTTACTATTTTGTTCT+TGG | - | chr6.2:84652825-84652844 | MS.gene05527:CDS | 25.0% |
| !! | CATTGTCTTCATTATTTTCT+TGG | + | chr6.2:84652788-84652807 | None:intergenic | 25.0% |
| TGCTTATTCTCTTATGTTGG+TGG | - | chr6.2:84652936-84652955 | MS.gene05527:CDS | 35.0% | |
| TTCTTGGCAATTTCTCACAT+GGG | - | chr6.2:84652841-84652860 | MS.gene05527:CDS | 35.0% | |
| AGAATCTGAGCATCACTAGT+AGG | + | chr6.2:84652904-84652923 | None:intergenic | 40.0% | |
| GTTCTTGGCAATTTCTCACA+TGG | - | chr6.2:84652840-84652859 | MS.gene05527:CDS | 40.0% | |
| TACTAGTGATGCTCAGATTC+TGG | - | chr6.2:84652903-84652922 | MS.gene05527:CDS | 40.0% | |
| GATGCTCAGATTCTGGATCA+AGG | - | chr6.2:84652910-84652929 | MS.gene05527:CDS | 45.0% | |
| TGAGCATCACTAGTAGGAGT+AGG | + | chr6.2:84652898-84652917 | None:intergenic | 45.0% | |
| TCACTAGTAGGAGTAGGTGC+TGG | + | chr6.2:84652892-84652911 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.2 | gene | 84652775 | 84652963 | 84652775 | ID=MS.gene05527 |
| chr6.2 | mRNA | 84652775 | 84652963 | 84652775 | ID=MS.gene05527.t1;Parent=MS.gene05527 |
| chr6.2 | exon | 84652775 | 84652963 | 84652775 | ID=MS.gene05527.t1.exon1;Parent=MS.gene05527.t1 |
| chr6.2 | CDS | 84652775 | 84652963 | 84652775 | ID=cds.MS.gene05527.t1;Parent=MS.gene05527.t1 |
| Gene Sequence |
| Protein sequence |
