Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61445.t1 | RHN63874.1 | 98.6 | 496 | 7 | 0 | 11 | 506 | 1 | 496 | 2.50E-282 | 980.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61445.t1 | O48780 | 83.7 | 498 | 81 | 0 | 9 | 506 | 8 | 505 | 1.1e-249 | 863.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61445.t1 | A0A396IHT1 | 98.6 | 496 | 7 | 0 | 11 | 506 | 1 | 496 | 1.8e-282 | 980.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056959 | MS.gene61445 | 0.978081 | 4.39E-145 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61445.t1 | MTR_4g113280 | 96.371 | 496 | 7 | 1 | 11 | 506 | 1 | 485 | 0.0 | 984 |
| MS.gene61445.t1 | MTR_2g054940 | 88.560 | 507 | 57 | 1 | 1 | 506 | 1 | 507 | 0.0 | 943 |
| MS.gene61445.t1 | MTR_7g081695 | 85.458 | 502 | 60 | 2 | 1 | 501 | 1 | 490 | 0.0 | 887 |
| MS.gene61445.t1 | MTR_1g076430 | 75.152 | 495 | 123 | 0 | 12 | 506 | 14 | 508 | 0.0 | 801 |
| MS.gene61445.t1 | MTR_1g041085 | 75.869 | 489 | 118 | 0 | 18 | 506 | 26 | 514 | 0.0 | 790 |
| MS.gene61445.t1 | MTR_4g070270 | 67.276 | 492 | 160 | 1 | 13 | 504 | 12 | 502 | 0.0 | 707 |
| MS.gene61445.t1 | MTR_7g118170 | 64.940 | 502 | 161 | 5 | 16 | 505 | 31 | 529 | 0.0 | 668 |
| MS.gene61445.t1 | MTR_1g110930 | 60.204 | 490 | 192 | 2 | 18 | 506 | 5 | 492 | 0.0 | 622 |
| MS.gene61445.t1 | MTR_3g105550 | 56.088 | 501 | 208 | 4 | 16 | 504 | 36 | 536 | 0.0 | 613 |
| MS.gene61445.t1 | MTR_5g056510 | 58.776 | 490 | 199 | 2 | 18 | 506 | 5 | 492 | 0.0 | 609 |
| MS.gene61445.t1 | MTR_6g074880 | 60.508 | 433 | 170 | 1 | 75 | 506 | 38 | 470 | 0.0 | 549 |
| MS.gene61445.t1 | MTR_4g054630 | 57.082 | 466 | 197 | 3 | 42 | 504 | 1 | 466 | 0.0 | 545 |
| MS.gene61445.t1 | MTR_4g054415 | 60.329 | 426 | 167 | 2 | 80 | 504 | 1 | 425 | 0.0 | 540 |
| MS.gene61445.t1 | MTR_6g032965 | 60.417 | 384 | 152 | 0 | 122 | 505 | 2 | 385 | 3.26e-175 | 498 |
| MS.gene61445.t1 | MTR_2g013740 | 53.333 | 480 | 214 | 4 | 18 | 496 | 33 | 503 | 3.77e-172 | 495 |
| MS.gene61445.t1 | MTR_7g118160 | 55.446 | 404 | 136 | 3 | 101 | 502 | 56 | 417 | 1.09e-160 | 463 |
| MS.gene61445.t1 | MTR_2g007020 | 51.707 | 410 | 195 | 3 | 97 | 505 | 45 | 452 | 3.08e-154 | 447 |
| MS.gene61445.t1 | MTR_2g436480 | 49.180 | 427 | 212 | 4 | 81 | 504 | 27 | 451 | 3.36e-150 | 437 |
| MS.gene61445.t1 | MTR_1g102290 | 46.078 | 408 | 219 | 1 | 96 | 502 | 77 | 484 | 5.26e-132 | 392 |
| MS.gene61445.t1 | MTR_3g072940 | 45.012 | 411 | 224 | 2 | 93 | 502 | 74 | 483 | 1.87e-125 | 375 |
| MS.gene61445.t1 | MTR_3g072980 | 45.012 | 411 | 224 | 2 | 93 | 502 | 74 | 483 | 2.43e-125 | 375 |
| MS.gene61445.t1 | MTR_6g051440 | 44.853 | 408 | 223 | 2 | 96 | 502 | 76 | 482 | 5.24e-116 | 351 |
| MS.gene61445.t1 | MTR_4g062270 | 39.817 | 437 | 245 | 5 | 75 | 501 | 5 | 433 | 3.98e-108 | 330 |
| MS.gene61445.t1 | MTR_1g115560 | 37.778 | 450 | 251 | 5 | 80 | 503 | 7 | 453 | 1.62e-104 | 321 |
| MS.gene61445.t1 | MTR_4g062190 | 37.360 | 447 | 253 | 5 | 72 | 501 | 6 | 442 | 2.11e-104 | 320 |
| MS.gene61445.t1 | MTR_2g096210 | 38.182 | 440 | 250 | 5 | 80 | 501 | 9 | 444 | 8.33e-104 | 319 |
| MS.gene61445.t1 | MTR_4g062170 | 38.864 | 440 | 253 | 4 | 75 | 501 | 5 | 441 | 5.02e-103 | 317 |
| MS.gene61445.t1 | MTR_4g054260 | 55.078 | 256 | 97 | 2 | 250 | 504 | 87 | 325 | 1.60e-94 | 290 |
| MS.gene61445.t1 | MTR_4g053905 | 66.038 | 159 | 54 | 0 | 346 | 504 | 1 | 159 | 5.12e-73 | 229 |
| MS.gene61445.t1 | MTR_4g054265 | 48.305 | 118 | 60 | 1 | 80 | 197 | 1 | 117 | 2.58e-31 | 117 |
| MS.gene61445.t1 | MTR_7g086260 | 59.615 | 52 | 21 | 0 | 344 | 395 | 25 | 76 | 1.30e-13 | 66.6 |
| MS.gene61445.t1 | MTR_3g086260 | 25.651 | 269 | 155 | 10 | 227 | 483 | 154 | 389 | 4.17e-12 | 68.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61445.t1 | AT2G26640 | 83.735 | 498 | 81 | 0 | 9 | 506 | 8 | 505 | 0.0 | 895 |
| MS.gene61445.t1 | AT1G04220 | 71.457 | 501 | 134 | 5 | 14 | 506 | 18 | 517 | 0.0 | 746 |
| MS.gene61445.t1 | AT5G43760 | 73.006 | 489 | 126 | 3 | 24 | 506 | 34 | 522 | 0.0 | 717 |
| MS.gene61445.t1 | AT2G16280 | 67.073 | 492 | 161 | 1 | 13 | 504 | 20 | 510 | 0.0 | 709 |
| MS.gene61445.t1 | AT1G19440 | 67.828 | 488 | 156 | 1 | 15 | 502 | 26 | 512 | 0.0 | 709 |
| MS.gene61445.t1 | AT1G01120 | 63.030 | 495 | 177 | 3 | 16 | 504 | 32 | 526 | 0.0 | 667 |
| MS.gene61445.t1 | AT1G68530 | 59.476 | 496 | 198 | 2 | 11 | 506 | 1 | 493 | 0.0 | 635 |
| MS.gene61445.t1 | AT4G34510 | 62.213 | 479 | 170 | 4 | 29 | 504 | 15 | 485 | 0.0 | 631 |
| MS.gene61445.t1 | AT1G25450 | 59.305 | 489 | 198 | 1 | 18 | 506 | 1 | 488 | 0.0 | 618 |
| MS.gene61445.t1 | AT2G26250 | 54.264 | 516 | 203 | 5 | 16 | 502 | 37 | 548 | 0.0 | 591 |
| MS.gene61445.t1 | AT4G34520 | 57.407 | 486 | 195 | 3 | 30 | 504 | 6 | 490 | 0.0 | 570 |
| MS.gene61445.t1 | AT2G15090 | 58.936 | 470 | 182 | 4 | 44 | 504 | 12 | 479 | 0.0 | 562 |
| MS.gene61445.t1 | AT4G34250 | 55.992 | 484 | 206 | 3 | 27 | 504 | 5 | 487 | 0.0 | 560 |
| MS.gene61445.t1 | AT2G46720 | 58.636 | 440 | 175 | 3 | 71 | 504 | 27 | 465 | 0.0 | 550 |
| MS.gene61445.t1 | AT3G10280 | 57.500 | 440 | 173 | 4 | 71 | 504 | 27 | 458 | 0.0 | 530 |
| MS.gene61445.t1 | AT1G71160 | 47.406 | 424 | 221 | 2 | 79 | 502 | 25 | 446 | 8.99e-146 | 426 |
| MS.gene61445.t1 | AT5G49070 | 48.605 | 430 | 213 | 4 | 80 | 505 | 31 | 456 | 2.79e-144 | 422 |
| MS.gene61445.t1 | AT1G68530 | 53.846 | 351 | 160 | 1 | 11 | 361 | 1 | 349 | 1.52e-140 | 410 |
| MS.gene61445.t1 | AT3G52160 | 44.219 | 493 | 224 | 8 | 1 | 493 | 8 | 449 | 1.84e-139 | 410 |
| MS.gene61445.t1 | AT3G52160 | 49.176 | 364 | 154 | 2 | 130 | 493 | 4 | 336 | 6.52e-127 | 374 |
| MS.gene61445.t1 | AT5G04530 | 40.244 | 410 | 235 | 4 | 99 | 501 | 31 | 437 | 2.44e-107 | 328 |
| MS.gene61445.t1 | AT2G28630 | 40.233 | 430 | 218 | 7 | 99 | 501 | 29 | 446 | 1.25e-106 | 327 |
| MS.gene61445.t1 | AT2G28630 | 40.233 | 430 | 218 | 7 | 99 | 501 | 29 | 446 | 1.25e-106 | 327 |
| MS.gene61445.t1 | AT1G07720 | 40.898 | 423 | 230 | 5 | 97 | 501 | 27 | 447 | 1.42e-104 | 321 |
| MS.gene61445.t1 | AT1G07720 | 40.898 | 423 | 230 | 5 | 97 | 501 | 70 | 490 | 3.79e-104 | 322 |
| MS.gene61445.t1 | AT5G49070 | 43.662 | 284 | 159 | 1 | 80 | 363 | 31 | 313 | 5.28e-80 | 252 |
| MS.gene61445.t1 | AT4G34850 | 25.391 | 256 | 153 | 9 | 235 | 481 | 91 | 317 | 1.33e-12 | 69.3 |
| MS.gene61445.t1 | AT4G34850 | 25.391 | 256 | 153 | 9 | 235 | 481 | 164 | 390 | 2.96e-12 | 68.9 |
Find 116 sgRNAs with CRISPR-Local
Find 133 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCACATTGAATTGGTATTT+TGG | 0.124443 | 8.4:-14965898 | MS.gene61445:CDS |
| TGCCATTGGCATCTTCTTCT+TGG | 0.201240 | 8.4:+14965713 | None:intergenic |
| GGCAAGTTCCTGGATGAATT+TGG | 0.256583 | 8.4:+14966663 | None:intergenic |
| GGAGCATCTACAATACAATT+TGG | 0.261765 | 8.4:-14966490 | MS.gene61445:CDS |
| GTGCACGAAAAGGAAATTTA+TGG | 0.264464 | 8.4:-14966349 | MS.gene61445:CDS |
| TGCCGGAGGTAGAGCTGTTT+TGG | 0.290721 | 8.4:-14965485 | MS.gene61445:CDS |
| AGGATGACACTTTATCGTTT+TGG | 0.293129 | 8.4:-14965412 | MS.gene61445:CDS |
| TCCCTTCTTAATTCTCCCTT+TGG | 0.293276 | 8.4:+14965342 | None:intergenic |
| ATGACCAATGCATAGGAATT+AGG | 0.295997 | 8.4:+14965934 | None:intergenic |
| ATCAGAATTCCTATATCTTT+AGG | 0.301992 | 8.4:+14966117 | None:intergenic |
| TACCTTGTCAAAAGATCTTA+TGG | 0.307989 | 8.4:-14965680 | MS.gene61445:CDS |
| AGCTTGAAATCAGGAATATA+AGG | 0.312020 | 8.4:+14965532 | None:intergenic |
| TTAACAGATTTCTTGAAATC+AGG | 0.316167 | 8.4:+14966642 | None:intergenic |
| TTTCTAGCTTCTTTCATTGA+AGG | 0.316291 | 8.4:+14966201 | None:intergenic |
| CTTGAAATCAGGCAAGTTCC+TGG | 0.317830 | 8.4:+14966653 | None:intergenic |
| CCACAAGTGGGGAAAGAAAA+AGG | 0.317889 | 8.4:+14966568 | None:intergenic |
| GCTTCTTTCATTGAAGGATT+AGG | 0.319754 | 8.4:+14966207 | None:intergenic |
| CGATAAAGTGTCATCCTTGA+TGG | 0.332202 | 8.4:+14965418 | None:intergenic |
| GGTGGGATGGGTTGCAGTGC+GGG | 0.335773 | 8.4:-14966000 | MS.gene61445:CDS |
| TGGCAAATAGCATTTGGTTC+TGG | 0.340846 | 8.4:-14965310 | MS.gene61445:CDS |
| GCCATTGGCATCTTCTTCTT+GGG | 0.342521 | 8.4:+14965714 | None:intergenic |
| TCAACAAGGTACACCGGTTT+CGG | 0.345198 | 8.4:+14966405 | None:intergenic |
| GAAGATGCCAATGGCAATAT+TGG | 0.352257 | 8.4:-14965706 | MS.gene61445:CDS |
| GTGCACTTACGCGATTCTTC+AGG | 0.355117 | 8.4:+14966366 | None:intergenic |
| CAGTTCACTTTGGTATGAAT+TGG | 0.362955 | 8.4:-14965377 | MS.gene61445:CDS |
| TTCTAATTGTTTGTTTCGAA+TGG | 0.382081 | 8.4:-14965854 | MS.gene61445:CDS |
| CCCAAATATCATAAAAGTCT+TGG | 0.400754 | 8.4:+14966511 | None:intergenic |
| ACAGTAAAGCCTAAAGATAT+AGG | 0.416419 | 8.4:-14966126 | MS.gene61445:CDS |
| AAAGATCTTATGGCAGTTGC+CGG | 0.419635 | 8.4:-14965670 | MS.gene61445:CDS |
| AGAACATGGCAAATAGCATT+TGG | 0.419756 | 8.4:-14965316 | MS.gene61445:CDS |
| GATACCTTTGGAACATCAAC+AGG | 0.425393 | 8.4:+14965196 | None:intergenic |
| TTGGAACATCAACAGGAAAC+TGG | 0.427742 | 8.4:+14965203 | None:intergenic |
| TCTAATTGTTTGTTTCGAAT+GGG | 0.432398 | 8.4:-14965853 | MS.gene61445:CDS |
| TGGAGAATATCACATTGAAT+TGG | 0.437805 | 8.4:-14965906 | MS.gene61445:CDS |
| TGCGAACCGTGGTGATCAAT+TGG | 0.438062 | 8.4:+14965776 | None:intergenic |
| TTGAAGATAGCGACGGAGTT+GGG | 0.440924 | 8.4:+14966073 | None:intergenic |
| AAATTGAAGTATGTTAAACT+TGG | 0.445451 | 8.4:-14966621 | MS.gene61445:CDS |
| GCAGCTACCACCATGGCATA+TGG | 0.451559 | 8.4:-14965443 | MS.gene61445:CDS |
| CGAAAGATTCTTGAGAGATC+GGG | 0.455522 | 8.4:-14966276 | MS.gene61445:CDS |
| CTGTTCTTCACCACATTGGT+TGG | 0.466086 | 8.4:-14965583 | MS.gene61445:CDS |
| TTCTTGAGAGATCGGGACTC+GGG | 0.466115 | 8.4:-14966269 | MS.gene61445:CDS |
| ATTCTTGAGAGATCGGGACT+CGG | 0.466508 | 8.4:-14966270 | MS.gene61445:CDS |
| GGAAAGTTAAGAACAGCCTC+TGG | 0.468115 | 8.4:+14966231 | None:intergenic |
| TCAAAAGCTAGCTTGAAATC+AGG | 0.468264 | 8.4:+14965523 | None:intergenic |
| GCGAAAGATTCTTGAGAGAT+CGG | 0.468284 | 8.4:-14966277 | MS.gene61445:CDS |
| AACAGTAGTTGTTCTGATGT+TGG | 0.477505 | 8.4:+14965601 | None:intergenic |
| TGTTCTTCACCACATTGGTT+GGG | 0.482146 | 8.4:-14965582 | MS.gene61445:CDS |
| GTGCAGTGTGGAAGGCTCTC+AGG | 0.489224 | 8.4:-14965273 | MS.gene61445:CDS |
| AGCTACAACCTAGGTGGGAT+GGG | 0.489907 | 8.4:-14966012 | MS.gene61445:CDS |
| AAAACTAATATCACTACGCT+AGG | 0.493178 | 8.4:-14965637 | MS.gene61445:CDS |
| TATCACTATCTAATCACTCA+TGG | 0.499740 | 8.4:-14966597 | MS.gene61445:CDS |
| CTGGATGAATTTGGCATCAA+GGG | 0.502606 | 8.4:+14966672 | None:intergenic |
| AGCCAAAGGGAGAATTAAGA+AGG | 0.519483 | 8.4:-14965344 | MS.gene61445:CDS |
| CCTGGATGAATTTGGCATCA+AGG | 0.520908 | 8.4:+14966671 | None:intergenic |
| ATTGAAGATAGCGACGGAGT+TGG | 0.522625 | 8.4:+14966072 | None:intergenic |
| GTCAATCATTACAAGCTTCG+AGG | 0.523795 | 8.4:-14966045 | MS.gene61445:CDS |
| AATATCATAAAAGTCTTGGA+TGG | 0.525987 | 8.4:+14966515 | None:intergenic |
| CTTACTAAATAGCTCATCAA+TGG | 0.525994 | 8.4:+14966152 | None:intergenic |
| CTATGCATTGGTCATTAGCA+TGG | 0.531088 | 8.4:-14965926 | MS.gene61445:CDS |
| GGAAATTTATGGATCAGTCT+AGG | 0.532242 | 8.4:-14966338 | MS.gene61445:CDS |
| CATAGGAATTAGGATTGGCC+TGG | 0.532315 | 8.4:+14965944 | None:intergenic |
| ATCCAAAACAGCTCTACCTC+CGG | 0.535564 | 8.4:+14965483 | None:intergenic |
| CAATCCTAATTCCTATGCAT+TGG | 0.538699 | 8.4:-14965938 | MS.gene61445:CDS |
| AATCGCGTAAGTGCACGAAA+AGG | 0.539987 | 8.4:-14966359 | MS.gene61445:CDS |
| GTGCAACAGTGCAGTGTGGA+AGG | 0.541767 | 8.4:-14965281 | MS.gene61445:CDS |
| GAGCTACAACCTAGGTGGGA+TGG | 0.541792 | 8.4:-14966013 | MS.gene61445:CDS |
| CATGGCATATGGAGCCATCA+AGG | 0.542439 | 8.4:-14965432 | MS.gene61445:CDS |
| AGGTGGGATGGGTTGCAGTG+CGG | 0.542640 | 8.4:-14966001 | MS.gene61445:CDS |
| TCTTTCATTGAAGGATTAGG+AGG | 0.545431 | 8.4:+14966210 | None:intergenic |
| GTGGGATGGGTTGCAGTGCG+GGG | 0.548925 | 8.4:-14965999 | MS.gene61445:CDS |
| GTTTCCTGTTGATGTTCCAA+AGG | 0.557767 | 8.4:-14965200 | None:intergenic |
| ACACATCTAGCAGTTCACTT+TGG | 0.560068 | 8.4:-14965387 | MS.gene61445:CDS |
| TGGTCAATCTCATCTATCCA+AGG | 0.561417 | 8.4:+14965223 | None:intergenic |
| GTTTCGAATGGGAGGAGCTG+TGG | 0.564123 | 8.4:-14965842 | MS.gene61445:CDS |
| GAAGCTGAGACTGTGATGTT+CGG | 0.565891 | 8.4:-14966177 | MS.gene61445:CDS |
| TCAATCATTACAAGCTTCGA+GGG | 0.569665 | 8.4:-14966044 | MS.gene61445:CDS |
| ACTACTGTTCTTCACCACAT+TGG | 0.569759 | 8.4:-14965587 | MS.gene61445:CDS |
| AATTGTTTGTTTCGAATGGG+AGG | 0.571309 | 8.4:-14965850 | MS.gene61445:CDS |
| AATATAAAGAGCTACAACCT+AGG | 0.575611 | 8.4:-14966021 | MS.gene61445:CDS |
| CAGGACAATCAACCCTGTGA+AGG | 0.575639 | 8.4:-14965254 | MS.gene61445:CDS |
| TCTTGCTAAAGATCTTCTCC+AGG | 0.579124 | 8.4:-14965962 | MS.gene61445:CDS |
| GCAGCAATCAACACCACAAG+TGG | 0.584783 | 8.4:+14966555 | None:intergenic |
| TAAAGAGCTACAACCTAGGT+GGG | 0.586492 | 8.4:-14966017 | MS.gene61445:CDS |
| TGGCTTACACAGAAGCCAAA+GGG | 0.588822 | 8.4:-14965357 | MS.gene61445:CDS |
| GGTAACACCAATATTGCCAT+TGG | 0.592052 | 8.4:+14965699 | None:intergenic |
| AAACAGCTCTACCTCCGGCA+TGG | 0.599316 | 8.4:+14965488 | None:intergenic |
| CCTTGATGCCAAATTCATCC+AGG | 0.603328 | 8.4:-14966671 | MS.gene61445:CDS |
| CAATGCATAGGAATTAGGAT+TGG | 0.604720 | 8.4:+14965939 | None:intergenic |
| CACCACGGTTCGCACTAACA+AGG | 0.615650 | 8.4:-14965767 | MS.gene61445:CDS |
| TCTTGAGAGATCGGGACTCG+GGG | 0.615846 | 8.4:-14966268 | MS.gene61445:CDS |
| GCCAAAGGGAGAATTAAGAA+GGG | 0.617206 | 8.4:-14965343 | MS.gene61445:CDS |
| CATGCTAATGACCAATGCAT+AGG | 0.618448 | 8.4:+14965927 | None:intergenic |
| TATATCTTTAGGCTTTACTG+TGG | 0.620510 | 8.4:+14966128 | None:intergenic |
| TTGGCTTACACAGAAGCCAA+AGG | 0.624548 | 8.4:-14965358 | MS.gene61445:CDS |
| CTGTGAAGGAGAAAAGCCCT+TGG | 0.625413 | 8.4:-14965240 | MS.gene61445:CDS |
| AAACTAATATCACTACGCTA+GGG | 0.626894 | 8.4:-14965636 | MS.gene61445:CDS |
| GGTCAATCTCATCTATCCAA+GGG | 0.627776 | 8.4:+14965224 | None:intergenic |
| CTTGATGGCTCCATATGCCA+TGG | 0.634786 | 8.4:+14965433 | None:intergenic |
| CAGCAATCAACACCACAAGT+GGG | 0.638117 | 8.4:+14966556 | None:intergenic |
| CACTGCAACCCATCCCACCT+AGG | 0.640569 | 8.4:+14966004 | None:intergenic |
| ATAAAGAGCTACAACCTAGG+TGG | 0.642158 | 8.4:-14966018 | MS.gene61445:CDS |
| TCTGATGTTGGAAGTACGAG+AGG | 0.642235 | 8.4:+14965613 | None:intergenic |
| GGAATCTACTTATCTTCCAG+AGG | 0.644232 | 8.4:-14966247 | MS.gene61445:CDS |
| TTAAGAAGGGAGATAGAACA+TGG | 0.646961 | 8.4:-14965330 | MS.gene61445:CDS |
| AAAGCGTGGACAGGAAAACA+AGG | 0.654849 | 8.4:+14966442 | None:intergenic |
| AGCAATCAACACCACAAGTG+GGG | 0.662251 | 8.4:+14966557 | None:intergenic |
| ACCCAAGAAGAAGATGCCAA+TGG | 0.668165 | 8.4:-14965715 | MS.gene61445:CDS |
| AAGTTTCTTCCCAACCAATG+TGG | 0.668173 | 8.4:+14965573 | None:intergenic |
| ACAATCATTGAAGATAGCGA+CGG | 0.668618 | 8.4:+14966066 | None:intergenic |
| GAGAAATCAACAAGGTACAC+CGG | 0.678354 | 8.4:+14966399 | None:intergenic |
| AAAACTTGCAGCTACCACCA+TGG | 0.691925 | 8.4:-14965450 | MS.gene61445:CDS |
| TCAAGTGCAACAGTGCAGTG+TGG | 0.695312 | 8.4:-14965285 | MS.gene61445:CDS |
| GATGGCTCCATATGCCATGG+TGG | 0.697694 | 8.4:+14965436 | None:intergenic |
| AGCCTTGTTAGTGCGAACCG+TGG | 0.715624 | 8.4:+14965765 | None:intergenic |
| AAAATACCAATTGATCACCA+CGG | 0.722409 | 8.4:-14965782 | MS.gene61445:CDS |
| TGTAACATGAGAAATCAACA+AGG | 0.794037 | 8.4:+14966391 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTGAAGTATGTTAAACT+TGG | - | chr8.4:14965276-14965295 | MS.gene61445:CDS | 20.0% |
| ! | AATATCATAAAAGTCTTGGA+TGG | + | chr8.4:14965385-14965404 | None:intergenic | 25.0% |
| ! | ATCAGAATTCCTATATCTTT+AGG | + | chr8.4:14965783-14965802 | None:intergenic | 25.0% |
| ! | TCTAATTGTTTGTTTCGAAT+GGG | - | chr8.4:14966044-14966063 | MS.gene61445:CDS | 25.0% |
| ! | TTAACAGATTTCTTGAAATC+AGG | + | chr8.4:14965258-14965277 | None:intergenic | 25.0% |
| ! | TTCTAATTGTTTGTTTCGAA+TGG | - | chr8.4:14966043-14966062 | MS.gene61445:CDS | 25.0% |
| !! | ATCACATTGAATTGGTATTT+TGG | - | chr8.4:14965999-14966018 | MS.gene61445:CDS | 25.0% |
| !! | CCAAGACTTTTATGATATTT+GGG | - | chr8.4:14965386-14965405 | MS.gene61445:CDS | 25.0% |
| !! | TCCAAGACTTTTATGATATT+TGG | - | chr8.4:14965385-14965404 | MS.gene61445:CDS | 25.0% |
| !!! | ATTAGTTTTTAAAGCATCAC+CGG | + | chr8.4:14966249-14966268 | None:intergenic | 25.0% |
| AAAACTAATATCACTACGCT+AGG | - | chr8.4:14966260-14966279 | MS.gene61445:CDS | 30.0% | |
| AAAATACCAATTGATCACCA+CGG | - | chr8.4:14966115-14966134 | MS.gene61445:CDS | 30.0% | |
| AAACTAATATCACTACGCTA+GGG | - | chr8.4:14966261-14966280 | MS.gene61445:CDS | 30.0% | |
| AATATAAAGAGCTACAACCT+AGG | - | chr8.4:14965876-14965895 | MS.gene61445:CDS | 30.0% | |
| ACAGTAAAGCCTAAAGATAT+AGG | - | chr8.4:14965771-14965790 | MS.gene61445:CDS | 30.0% | |
| AGCTTGAAATCAGGAATATA+AGG | + | chr8.4:14966368-14966387 | None:intergenic | 30.0% | |
| CCCAAATATCATAAAAGTCT+TGG | + | chr8.4:14965389-14965408 | None:intergenic | 30.0% | |
| CTTACTAAATAGCTCATCAA+TGG | + | chr8.4:14965748-14965767 | None:intergenic | 30.0% | |
| TACCTTGTCAAAAGATCTTA+TGG | - | chr8.4:14966217-14966236 | MS.gene61445:CDS | 30.0% | |
| TATATCTTTAGGCTTTACTG+TGG | + | chr8.4:14965772-14965791 | None:intergenic | 30.0% | |
| TATCACTATCTAATCACTCA+TGG | - | chr8.4:14965300-14965319 | MS.gene61445:CDS | 30.0% | |
| TGGAGAATATCACATTGAAT+TGG | - | chr8.4:14965991-14966010 | MS.gene61445:CDS | 30.0% | |
| TGTAACATGAGAAATCAACA+AGG | + | chr8.4:14965509-14965528 | None:intergenic | 30.0% | |
| ! | TAGGATGATCAGTACTTTTA+CGG | - | chr8.4:14965578-14965597 | MS.gene61445:CDS | 30.0% |
| !! | ATTGGTATTTTGGAAACGAT+CGG | - | chr8.4:14966009-14966028 | MS.gene61445:CDS | 30.0% |
| !! | TTTCTAGCTTCTTTCATTGA+AGG | + | chr8.4:14965699-14965718 | None:intergenic | 30.0% |
| AAAGTCTTGGATGGAAAAAG+TGG | + | chr8.4:14965376-14965395 | None:intergenic | 35.0% | |
| AACAGTAGTTGTTCTGATGT+TGG | + | chr8.4:14966299-14966318 | None:intergenic | 35.0% | |
| AATTGTTTGTTTCGAATGGG+AGG | - | chr8.4:14966047-14966066 | MS.gene61445:CDS | 35.0% | |
| ACAATCATTGAAGATAGCGA+CGG | + | chr8.4:14965834-14965853 | None:intergenic | 35.0% | |
| AGAACATGGCAAATAGCATT+TGG | - | chr8.4:14966581-14966600 | MS.gene61445:CDS | 35.0% | |
| ATGACCAATGCATAGGAATT+AGG | + | chr8.4:14965966-14965985 | None:intergenic | 35.0% | |
| CAATCCTAATTCCTATGCAT+TGG | - | chr8.4:14965959-14965978 | MS.gene61445:CDS | 35.0% | |
| CAATGCATAGGAATTAGGAT+TGG | + | chr8.4:14965961-14965980 | None:intergenic | 35.0% | |
| CAGTTCACTTTGGTATGAAT+TGG | - | chr8.4:14966520-14966539 | MS.gene61445:CDS | 35.0% | |
| GCTTCTTTCATTGAAGGATT+AGG | + | chr8.4:14965693-14965712 | None:intergenic | 35.0% | |
| GGAAATTTATGGATCAGTCT+AGG | - | chr8.4:14965559-14965578 | MS.gene61445:CDS | 35.0% | |
| GTGCACGAAAAGGAAATTTA+TGG | - | chr8.4:14965548-14965567 | MS.gene61445:CDS | 35.0% | |
| TCAATCATTACAAGCTTCGA+GGG | - | chr8.4:14965853-14965872 | MS.gene61445:CDS | 35.0% | |
| TCTTTCATTGAAGGATTAGG+AGG | + | chr8.4:14965690-14965709 | None:intergenic | 35.0% | |
| TTAAGAAGGGAGATAGAACA+TGG | - | chr8.4:14966567-14966586 | MS.gene61445:CDS | 35.0% | |
| ! | AGGATGACACTTTATCGTTT+TGG | - | chr8.4:14966485-14966504 | MS.gene61445:CDS | 35.0% |
| ! | GGAGCATCTACAATACAATT+TGG | - | chr8.4:14965407-14965426 | MS.gene61445:CDS | 35.0% |
| ! | TCAAAAGCTAGCTTGAAATC+AGG | + | chr8.4:14966377-14966396 | None:intergenic | 35.0% |
| ! | TGCCATAAGATCTTTTGACA+AGG | + | chr8.4:14966222-14966241 | None:intergenic | 35.0% |
| ! | TTTTGATCTTCTTCTGTCTG+AGG | + | chr8.4:14966099-14966118 | None:intergenic | 35.0% |
| !! | TCTTCTGTCTGAGGTTTTAT+TGG | + | chr8.4:14966090-14966109 | None:intergenic | 35.0% |
| AAAGATCTTATGGCAGTTGC+CGG | - | chr8.4:14966227-14966246 | MS.gene61445:CDS | 40.0% | |
| AAGTTTCTTCCCAACCAATG+TGG | + | chr8.4:14966327-14966346 | None:intergenic | 40.0% | |
| ACACATCTAGCAGTTCACTT+TGG | - | chr8.4:14966510-14966529 | MS.gene61445:CDS | 40.0% | |
| ACGAGTCAAAAAGTAAAGCG+TGG | + | chr8.4:14965472-14965491 | None:intergenic | 40.0% | |
| ACTACTGTTCTTCACCACAT+TGG | - | chr8.4:14966310-14966329 | MS.gene61445:CDS | 40.0% | |
| AGCCAAAGGGAGAATTAAGA+AGG | - | chr8.4:14966553-14966572 | MS.gene61445:CDS | 40.0% | |
| ATAAAGAGCTACAACCTAGG+TGG | - | chr8.4:14965879-14965898 | MS.gene61445:CDS | 40.0% | |
| CATGCTAATGACCAATGCAT+AGG | + | chr8.4:14965973-14965992 | None:intergenic | 40.0% | |
| CGAAAGATTCTTGAGAGATC+GGG | - | chr8.4:14965621-14965640 | MS.gene61445:CDS | 40.0% | |
| CTGGATGAATTTGGCATCAA+GGG | + | chr8.4:14965228-14965247 | None:intergenic | 40.0% | |
| GAAGATGCCAATGGCAATAT+TGG | - | chr8.4:14966191-14966210 | MS.gene61445:CDS | 40.0% | |
| GAGAAATCAACAAGGTACAC+CGG | + | chr8.4:14965501-14965520 | None:intergenic | 40.0% | |
| GCCAAAGGGAGAATTAAGAA+GGG | - | chr8.4:14966554-14966573 | MS.gene61445:CDS | 40.0% | |
| GCGAAAGATTCTTGAGAGAT+CGG | - | chr8.4:14965620-14965639 | MS.gene61445:CDS | 40.0% | |
| GGAATCTACTTATCTTCCAG+AGG | - | chr8.4:14965650-14965669 | MS.gene61445:CDS | 40.0% | |
| GGTAACACCAATATTGCCAT+TGG | + | chr8.4:14966201-14966220 | None:intergenic | 40.0% | |
| GGTCAATCTCATCTATCCAA+GGG | + | chr8.4:14966676-14966695 | None:intergenic | 40.0% | |
| GTCAATCATTACAAGCTTCG+AGG | - | chr8.4:14965852-14965871 | MS.gene61445:CDS | 40.0% | |
| TAAAGAGCTACAACCTAGGT+GGG | - | chr8.4:14965880-14965899 | MS.gene61445:CDS | 40.0% | |
| TCAAAAAGTAAAGCGTGGAC+AGG | + | chr8.4:14965467-14965486 | None:intergenic | 40.0% | |
| TCCCTTCTTAATTCTCCCTT+TGG | + | chr8.4:14966558-14966577 | None:intergenic | 40.0% | |
| TCTTGCTAAAGATCTTCTCC+AGG | - | chr8.4:14965935-14965954 | MS.gene61445:CDS | 40.0% | |
| TGGTCAATCTCATCTATCCA+AGG | + | chr8.4:14966677-14966696 | None:intergenic | 40.0% | |
| TTGGAACATCAACAGGAAAC+TGG | + | chr8.4:14966697-14966716 | None:intergenic | 40.0% | |
| ! | CGATAAAGTGTCATCCTTGA+TGG | + | chr8.4:14966482-14966501 | None:intergenic | 40.0% |
| ! | CTATGCATTGGTCATTAGCA+TGG | - | chr8.4:14965971-14965990 | MS.gene61445:CDS | 40.0% |
| ! | GAACATTTTTGCATCCATGC+CGG | - | chr8.4:14966395-14966414 | MS.gene61445:CDS | 40.0% |
| ! | GATGATCAGTACTTTTACGG+AGG | - | chr8.4:14965581-14965600 | MS.gene61445:CDS | 40.0% |
| ! | TGGCAAATAGCATTTGGTTC+TGG | - | chr8.4:14966587-14966606 | MS.gene61445:CDS | 40.0% |
| ! | TGTTCTTCACCACATTGGTT+GGG | - | chr8.4:14966315-14966334 | MS.gene61445:CDS | 40.0% |
| !! | TAGAGCTGTTTTGGATGAAC+TGG | - | chr8.4:14966421-14966440 | MS.gene61445:CDS | 40.0% |
| AAAACTTGCAGCTACCACCA+TGG | - | chr8.4:14966447-14966466 | MS.gene61445:CDS | 45.0% | |
| AAAGCGTGGACAGGAAAACA+AGG | + | chr8.4:14965458-14965477 | None:intergenic | 45.0% | |
| AATCGCGTAAGTGCACGAAA+AGG | - | chr8.4:14965538-14965557 | MS.gene61445:CDS | 45.0% | |
| ACCCAAGAAGAAGATGCCAA+TGG | - | chr8.4:14966182-14966201 | MS.gene61445:CDS | 45.0% | |
| AGCAATCAACACCACAAGTG+GGG | + | chr8.4:14965343-14965362 | None:intergenic | 45.0% | |
| ATCCAAAACAGCTCTACCTC+CGG | + | chr8.4:14966417-14966436 | None:intergenic | 45.0% | |
| ATTCTTGAGAGATCGGGACT+CGG | - | chr8.4:14965627-14965646 | MS.gene61445:CDS | 45.0% | |
| ATTGAAGATAGCGACGGAGT+TGG | + | chr8.4:14965828-14965847 | None:intergenic | 45.0% | |
| CAGCAATCAACACCACAAGT+GGG | + | chr8.4:14965344-14965363 | None:intergenic | 45.0% | |
| CCACAAGTGGGGAAAGAAAA+AGG | + | chr8.4:14965332-14965351 | None:intergenic | 45.0% | |
| CCTGGATGAATTTGGCATCA+AGG | + | chr8.4:14965229-14965248 | None:intergenic | 45.0% | |
| CCTTGATGCCAAATTCATCC+AGG | - | chr8.4:14965226-14965245 | MS.gene61445:CDS | 45.0% | |
| CTGTTCTTCACCACATTGGT+TGG | - | chr8.4:14966314-14966333 | MS.gene61445:CDS | 45.0% | |
| CTTGAAATCAGGCAAGTTCC+TGG | + | chr8.4:14965247-14965266 | None:intergenic | 45.0% | |
| GAAGCTGAGACTGTGATGTT+CGG | - | chr8.4:14965720-14965739 | MS.gene61445:CDS | 45.0% | |
| GCCATTGGCATCTTCTTCTT+GGG | + | chr8.4:14966186-14966205 | None:intergenic | 45.0% | |
| GGAAAGTTAAGAACAGCCTC+TGG | + | chr8.4:14965669-14965688 | None:intergenic | 45.0% | |
| GGCAAGTTCCTGGATGAATT+TGG | + | chr8.4:14965237-14965256 | None:intergenic | 45.0% | |
| TGCCATTGGCATCTTCTTCT+TGG | + | chr8.4:14966187-14966206 | None:intergenic | 45.0% | |
| TGGCTTACACAGAAGCCAAA+GGG | - | chr8.4:14966540-14966559 | MS.gene61445:CDS | 45.0% | |
| TTGAAGATAGCGACGGAGTT+GGG | + | chr8.4:14965827-14965846 | None:intergenic | 45.0% | |
| TTGGCTTACACAGAAGCCAA+AGG | - | chr8.4:14966539-14966558 | MS.gene61445:CDS | 45.0% | |
| ! | AAGGGCTTTTCTCCTTCACA+GGG | + | chr8.4:14966658-14966677 | None:intergenic | 45.0% |
| ! | CCTTTTTCTTTCCCCACTTG+TGG | - | chr8.4:14965329-14965348 | MS.gene61445:CDS | 45.0% |
| ! | TCTGATGTTGGAAGTACGAG+AGG | + | chr8.4:14966287-14966306 | None:intergenic | 45.0% |
| !! | CATAGGAATTAGGATTGGCC+TGG | + | chr8.4:14965956-14965975 | None:intergenic | 45.0% |
| !! | CTTTTTGACTCGTCCGAAAC+CGG | - | chr8.4:14965479-14965498 | MS.gene61445:CDS | 45.0% |
| !! | TCAACAAGGTACACCGGTTT+CGG | + | chr8.4:14965495-14965514 | None:intergenic | 45.0% |
| AGCTACAACCTAGGTGGGAT+GGG | - | chr8.4:14965885-14965904 | MS.gene61445:CDS | 50.0% | |
| CAGGACAATCAACCCTGTGA+AGG | - | chr8.4:14966643-14966662 | MS.gene61445:CDS | 50.0% | |
| CATGGCATATGGAGCCATCA+AGG | - | chr8.4:14966465-14966484 | MS.gene61445:CDS | 50.0% | |
| CTGTGAAGGAGAAAAGCCCT+TGG | - | chr8.4:14966657-14966676 | MS.gene61445:CDS | 50.0% | |
| GCAGCAATCAACACCACAAG+TGG | + | chr8.4:14965345-14965364 | None:intergenic | 50.0% | |
| GTGCACTTACGCGATTCTTC+AGG | + | chr8.4:14965534-14965553 | None:intergenic | 50.0% | |
| TCAAGTGCAACAGTGCAGTG+TGG | - | chr8.4:14966612-14966631 | MS.gene61445:CDS | 50.0% | |
| TGCGAACCGTGGTGATCAAT+TGG | + | chr8.4:14966124-14966143 | None:intergenic | 50.0% | |
| TTCTTGAGAGATCGGGACTC+GGG | - | chr8.4:14965628-14965647 | MS.gene61445:CDS | 50.0% | |
| ! | CAAGGGCTTTTCTCCTTCAC+AGG | + | chr8.4:14966659-14966678 | None:intergenic | 50.0% |
| ! | CATTTTTGCATCCATGCCGG+AGG | - | chr8.4:14966398-14966417 | MS.gene61445:CDS | 50.0% |
| ! | CTTGATGGCTCCATATGCCA+TGG | + | chr8.4:14966467-14966486 | None:intergenic | 50.0% |
| AAACAGCTCTACCTCCGGCA+TGG | + | chr8.4:14966412-14966431 | None:intergenic | 55.0% | |
| CACCACGGTTCGCACTAACA+AGG | - | chr8.4:14966130-14966149 | MS.gene61445:CDS | 55.0% | |
| GAGCTACAACCTAGGTGGGA+TGG | - | chr8.4:14965884-14965903 | MS.gene61445:CDS | 55.0% | |
| GATGGCTCCATATGCCATGG+TGG | + | chr8.4:14966464-14966483 | None:intergenic | 55.0% | |
| GCAGCTACCACCATGGCATA+TGG | - | chr8.4:14966454-14966473 | MS.gene61445:CDS | 55.0% | |
| GTGCAACAGTGCAGTGTGGA+AGG | - | chr8.4:14966616-14966635 | MS.gene61445:CDS | 55.0% | |
| GTTTCGAATGGGAGGAGCTG+TGG | - | chr8.4:14966055-14966074 | MS.gene61445:CDS | 55.0% | |
| TCTTGAGAGATCGGGACTCG+GGG | - | chr8.4:14965629-14965648 | MS.gene61445:CDS | 55.0% | |
| ! | TGCCGGAGGTAGAGCTGTTT+TGG | - | chr8.4:14966412-14966431 | MS.gene61445:CDS | 55.0% |
| !! | AGCCTTGTTAGTGCGAACCG+TGG | + | chr8.4:14966135-14966154 | None:intergenic | 55.0% |
| CACTGCAACCCATCCCACCT+AGG | + | chr8.4:14965896-14965915 | None:intergenic | 60.0% | |
| GTGCAGTGTGGAAGGCTCTC+AGG | - | chr8.4:14966624-14966643 | MS.gene61445:CDS | 60.0% | |
| !! | AGGTGGGATGGGTTGCAGTG+CGG | - | chr8.4:14965896-14965915 | MS.gene61445:CDS | 60.0% |
| !! | GGTGGGATGGGTTGCAGTGC+GGG | - | chr8.4:14965897-14965916 | MS.gene61445:CDS | 65.0% |
| !! | GTGGGATGGGTTGCAGTGCG+GGG | - | chr8.4:14965898-14965917 | MS.gene61445:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 14965201 | 14966718 | 14965201 | ID=MS.gene61445 |
| chr8.4 | mRNA | 14965201 | 14966718 | 14965201 | ID=MS.gene61445.t1;Parent=MS.gene61445 |
| chr8.4 | exon | 14965201 | 14966718 | 14965201 | ID=MS.gene61445.t1.exon1;Parent=MS.gene61445.t1 |
| chr8.4 | CDS | 14965201 | 14966718 | 14965201 | ID=cds.MS.gene61445.t1;Parent=MS.gene61445.t1 |
| Gene Sequence |
| Protein sequence |
