Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056959.t1 | RHN63874.1 | 98.8 | 500 | 6 | 0 | 11 | 510 | 1 | 500 | 9.40E-285 | 988.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056959.t1 | O48780 | 83.9 | 502 | 81 | 0 | 9 | 510 | 8 | 509 | 9.0e-252 | 870.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056959.t1 | A0A396IHT1 | 98.8 | 500 | 6 | 0 | 11 | 510 | 1 | 500 | 6.8e-285 | 988.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056959 | MS.gene063452 | 0.836134 | 1.12E-56 | -1.69E-46 |
| MS.gene056959 | MS.gene31203 | 0.805172 | 1.52E-49 | -1.69E-46 |
| MS.gene056959 | MS.gene31204 | 0.809966 | 1.46E-50 | -1.69E-46 |
| MS.gene056959 | MS.gene34420 | 0.818463 | 1.93E-52 | -1.69E-46 |
| MS.gene056959 | MS.gene38561 | 0.80738 | 5.20E-50 | -1.69E-46 |
| MS.gene056959 | MS.gene59912 | 0.977308 | 1.60E-143 | -1.69E-46 |
| MS.gene056959 | MS.gene59913 | 0.917743 | 3.66E-86 | -1.69E-46 |
| MS.gene056959 | MS.gene61445 | 0.978081 | 4.39E-145 | -1.69E-46 |
| MS.gene056959 | MS.gene72135 | 0.805805 | 1.12E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056959.t1 | MTR_4g113280 | 96.600 | 500 | 6 | 1 | 11 | 510 | 1 | 489 | 0.0 | 994 |
| MS.gene056959.t1 | MTR_2g054940 | 88.454 | 511 | 58 | 1 | 1 | 510 | 1 | 511 | 0.0 | 950 |
| MS.gene056959.t1 | MTR_7g081695 | 85.657 | 502 | 59 | 2 | 1 | 501 | 1 | 490 | 0.0 | 890 |
| MS.gene056959.t1 | MTR_1g076430 | 75.351 | 499 | 123 | 0 | 12 | 510 | 14 | 512 | 0.0 | 809 |
| MS.gene056959.t1 | MTR_1g041085 | 75.862 | 493 | 119 | 0 | 18 | 510 | 26 | 518 | 0.0 | 797 |
| MS.gene056959.t1 | MTR_4g070270 | 67.276 | 492 | 160 | 1 | 13 | 504 | 12 | 502 | 0.0 | 709 |
| MS.gene056959.t1 | MTR_7g118170 | 65.139 | 502 | 160 | 5 | 16 | 505 | 31 | 529 | 0.0 | 671 |
| MS.gene056959.t1 | MTR_1g110930 | 60.285 | 491 | 192 | 2 | 18 | 507 | 5 | 493 | 0.0 | 625 |
| MS.gene056959.t1 | MTR_3g105550 | 56.287 | 501 | 207 | 4 | 16 | 504 | 36 | 536 | 0.0 | 615 |
| MS.gene056959.t1 | MTR_5g056510 | 58.859 | 491 | 199 | 2 | 18 | 507 | 5 | 493 | 0.0 | 613 |
| MS.gene056959.t1 | MTR_6g074880 | 60.739 | 433 | 169 | 1 | 75 | 506 | 38 | 470 | 0.0 | 551 |
| MS.gene056959.t1 | MTR_4g054630 | 57.296 | 466 | 196 | 3 | 42 | 504 | 1 | 466 | 0.0 | 546 |
| MS.gene056959.t1 | MTR_4g054415 | 60.563 | 426 | 166 | 2 | 80 | 504 | 1 | 425 | 0.0 | 541 |
| MS.gene056959.t1 | MTR_6g032965 | 60.417 | 384 | 152 | 0 | 122 | 505 | 2 | 385 | 1.03e-175 | 500 |
| MS.gene056959.t1 | MTR_2g013740 | 53.333 | 480 | 214 | 4 | 18 | 496 | 33 | 503 | 1.55e-172 | 496 |
| MS.gene056959.t1 | MTR_7g118160 | 55.693 | 404 | 135 | 3 | 101 | 502 | 56 | 417 | 3.37e-161 | 464 |
| MS.gene056959.t1 | MTR_2g007020 | 51.707 | 410 | 195 | 3 | 97 | 505 | 45 | 452 | 2.97e-154 | 447 |
| MS.gene056959.t1 | MTR_2g436480 | 49.180 | 427 | 212 | 4 | 81 | 504 | 27 | 451 | 2.10e-150 | 438 |
| MS.gene056959.t1 | MTR_1g102290 | 46.078 | 408 | 219 | 1 | 96 | 502 | 77 | 484 | 2.45e-132 | 393 |
| MS.gene056959.t1 | MTR_3g072980 | 45.012 | 411 | 224 | 2 | 93 | 502 | 74 | 483 | 1.58e-125 | 375 |
| MS.gene056959.t1 | MTR_3g072940 | 45.012 | 411 | 224 | 2 | 93 | 502 | 74 | 483 | 1.58e-125 | 375 |
| MS.gene056959.t1 | MTR_6g051440 | 44.853 | 408 | 223 | 2 | 96 | 502 | 76 | 482 | 3.33e-116 | 352 |
| MS.gene056959.t1 | MTR_4g062270 | 40.275 | 437 | 243 | 5 | 75 | 501 | 5 | 433 | 9.05e-109 | 332 |
| MS.gene056959.t1 | MTR_4g062190 | 37.584 | 447 | 252 | 5 | 72 | 501 | 6 | 442 | 4.76e-105 | 322 |
| MS.gene056959.t1 | MTR_1g115560 | 37.778 | 450 | 251 | 5 | 80 | 503 | 7 | 453 | 7.29e-105 | 322 |
| MS.gene056959.t1 | MTR_2g096210 | 38.182 | 440 | 250 | 5 | 80 | 501 | 9 | 444 | 4.63e-104 | 320 |
| MS.gene056959.t1 | MTR_4g062170 | 39.091 | 440 | 252 | 4 | 75 | 501 | 5 | 441 | 1.68e-103 | 318 |
| MS.gene056959.t1 | MTR_4g054260 | 55.469 | 256 | 96 | 2 | 250 | 504 | 87 | 325 | 2.94e-95 | 292 |
| MS.gene056959.t1 | MTR_4g053905 | 66.667 | 159 | 53 | 0 | 346 | 504 | 1 | 159 | 1.03e-73 | 231 |
| MS.gene056959.t1 | MTR_4g054265 | 48.305 | 118 | 60 | 1 | 80 | 197 | 1 | 117 | 2.23e-31 | 117 |
| MS.gene056959.t1 | MTR_7g086260 | 59.615 | 52 | 21 | 0 | 344 | 395 | 25 | 76 | 1.42e-13 | 66.6 |
| MS.gene056959.t1 | MTR_3g086260 | 25.651 | 269 | 155 | 10 | 227 | 483 | 154 | 389 | 3.10e-12 | 68.6 |
| MS.gene056959.t1 | MTR_4g075560 | 27.049 | 244 | 141 | 9 | 236 | 471 | 164 | 378 | 3.07e-11 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056959.t1 | AT2G26640 | 83.865 | 502 | 81 | 0 | 9 | 510 | 8 | 509 | 0.0 | 904 |
| MS.gene056959.t1 | AT1G04220 | 71.287 | 505 | 136 | 5 | 14 | 510 | 18 | 521 | 0.0 | 751 |
| MS.gene056959.t1 | AT5G43760 | 72.819 | 493 | 128 | 3 | 24 | 510 | 34 | 526 | 0.0 | 723 |
| MS.gene056959.t1 | AT2G16280 | 67.276 | 492 | 160 | 1 | 13 | 504 | 20 | 510 | 0.0 | 711 |
| MS.gene056959.t1 | AT1G19440 | 68.033 | 488 | 155 | 1 | 15 | 502 | 26 | 512 | 0.0 | 711 |
| MS.gene056959.t1 | AT1G01120 | 63.030 | 495 | 177 | 3 | 16 | 504 | 32 | 526 | 0.0 | 668 |
| MS.gene056959.t1 | AT1G68530 | 59.759 | 497 | 197 | 2 | 11 | 507 | 1 | 494 | 0.0 | 639 |
| MS.gene056959.t1 | AT4G34510 | 62.422 | 479 | 169 | 4 | 29 | 504 | 15 | 485 | 0.0 | 633 |
| MS.gene056959.t1 | AT1G25450 | 59.592 | 490 | 197 | 1 | 18 | 507 | 1 | 489 | 0.0 | 624 |
| MS.gene056959.t1 | AT2G26250 | 54.457 | 516 | 202 | 5 | 16 | 502 | 37 | 548 | 0.0 | 593 |
| MS.gene056959.t1 | AT4G34520 | 57.613 | 486 | 194 | 3 | 30 | 504 | 6 | 490 | 0.0 | 573 |
| MS.gene056959.t1 | AT2G15090 | 59.149 | 470 | 181 | 4 | 44 | 504 | 12 | 479 | 0.0 | 564 |
| MS.gene056959.t1 | AT4G34250 | 55.992 | 484 | 206 | 3 | 27 | 504 | 5 | 487 | 0.0 | 562 |
| MS.gene056959.t1 | AT2G46720 | 58.864 | 440 | 174 | 3 | 71 | 504 | 27 | 465 | 0.0 | 552 |
| MS.gene056959.t1 | AT3G10280 | 57.727 | 440 | 172 | 4 | 71 | 504 | 27 | 458 | 0.0 | 533 |
| MS.gene056959.t1 | AT1G71160 | 47.406 | 424 | 221 | 2 | 79 | 502 | 25 | 446 | 6.61e-146 | 427 |
| MS.gene056959.t1 | AT5G49070 | 48.276 | 435 | 217 | 4 | 80 | 510 | 31 | 461 | 4.74e-145 | 424 |
| MS.gene056959.t1 | AT1G68530 | 53.846 | 351 | 160 | 1 | 11 | 361 | 1 | 349 | 8.87e-141 | 410 |
| MS.gene056959.t1 | AT3G52160 | 44.219 | 493 | 224 | 8 | 1 | 493 | 8 | 449 | 6.59e-140 | 411 |
| MS.gene056959.t1 | AT3G52160 | 49.176 | 364 | 154 | 2 | 130 | 493 | 4 | 336 | 2.89e-127 | 374 |
| MS.gene056959.t1 | AT5G04530 | 40.244 | 410 | 235 | 4 | 99 | 501 | 31 | 437 | 1.18e-107 | 329 |
| MS.gene056959.t1 | AT2G28630 | 40.233 | 430 | 218 | 7 | 99 | 501 | 29 | 446 | 4.78e-107 | 328 |
| MS.gene056959.t1 | AT2G28630 | 40.233 | 430 | 218 | 7 | 99 | 501 | 29 | 446 | 4.78e-107 | 328 |
| MS.gene056959.t1 | AT1G07720 | 40.898 | 423 | 230 | 5 | 97 | 501 | 27 | 447 | 8.00e-105 | 322 |
| MS.gene056959.t1 | AT1G07720 | 40.898 | 423 | 230 | 5 | 97 | 501 | 70 | 490 | 1.33e-104 | 323 |
| MS.gene056959.t1 | AT5G49070 | 43.662 | 284 | 159 | 1 | 80 | 363 | 31 | 313 | 4.81e-80 | 253 |
| MS.gene056959.t1 | AT4G34850 | 25.781 | 256 | 152 | 9 | 235 | 481 | 91 | 317 | 2.81e-13 | 71.2 |
| MS.gene056959.t1 | AT4G34850 | 25.781 | 256 | 152 | 9 | 235 | 481 | 164 | 390 | 6.27e-13 | 70.9 |
Find 117 sgRNAs with CRISPR-Local
Find 134 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCACATTGAATTGGTATTT+TGG | 0.124443 | 8.4:-14812282 | MS.gene056959:CDS |
| TGCCATTGGCATCTTCTTCT+TGG | 0.201240 | 8.4:+14812097 | None:intergenic |
| GGCAAGTTCCTGGATGAATT+TGG | 0.256583 | 8.4:+14813047 | None:intergenic |
| GGAGCATCTACAATACAATT+TGG | 0.261765 | 8.4:-14812874 | MS.gene056959:CDS |
| GTGCACGAAAAGGAAATTTA+TGG | 0.264464 | 8.4:-14812733 | MS.gene056959:CDS |
| TGCCGGAGGTAGAGCTGTTT+TGG | 0.290721 | 8.4:-14811869 | MS.gene056959:CDS |
| AGGATGACACTTTATCGTTT+TGG | 0.293129 | 8.4:-14811796 | MS.gene056959:CDS |
| TCCCTTCTTAATTCTCCCTT+TGG | 0.293276 | 8.4:+14811726 | None:intergenic |
| ATGACCAATGCATAGGAATT+AGG | 0.295997 | 8.4:+14812318 | None:intergenic |
| ATCAGAATTCCTATATCTTT+AGG | 0.301992 | 8.4:+14812501 | None:intergenic |
| TACCTTGTCAAAAGATCTTA+TGG | 0.307989 | 8.4:-14812064 | MS.gene056959:CDS |
| AGCTTGAAATCAGGAATATA+AGG | 0.312020 | 8.4:+14811916 | None:intergenic |
| TTAACAGATTTCTTGAAATC+AGG | 0.316167 | 8.4:+14813026 | None:intergenic |
| TTTCTAGCTTCTTTCATTGA+AGG | 0.316291 | 8.4:+14812585 | None:intergenic |
| CTTGAAATCAGGCAAGTTCC+TGG | 0.317830 | 8.4:+14813037 | None:intergenic |
| CCACAAGTGGGGAAAGAAAA+AGG | 0.317889 | 8.4:+14812952 | None:intergenic |
| GCTTCTTTCATTGAAGGATT+AGG | 0.319754 | 8.4:+14812591 | None:intergenic |
| CGATAAAGTGTCATCCTTGA+TGG | 0.332202 | 8.4:+14811802 | None:intergenic |
| GGTGGGATGGGTTGCAGTGC+GGG | 0.335773 | 8.4:-14812384 | MS.gene056959:CDS |
| TGGCAAATAGCATTTGGTTC+TGG | 0.340846 | 8.4:-14811694 | MS.gene056959:CDS |
| GCCATTGGCATCTTCTTCTT+GGG | 0.342521 | 8.4:+14812098 | None:intergenic |
| TCAACAAGGTACACCGGTTT+CGG | 0.345198 | 8.4:+14812789 | None:intergenic |
| GAAGATGCCAATGGCAATAT+TGG | 0.352257 | 8.4:-14812090 | MS.gene056959:CDS |
| GTGCACTTACGCGATTCTTC+AGG | 0.355117 | 8.4:+14812750 | None:intergenic |
| CAGTTCACTTTGGTATGAAT+TGG | 0.362955 | 8.4:-14811761 | MS.gene056959:CDS |
| TTCTAATTGTTTGTTTCGAA+TGG | 0.382081 | 8.4:-14812238 | MS.gene056959:CDS |
| CCCAAATATCATAAAAGTCT+TGG | 0.400754 | 8.4:+14812895 | None:intergenic |
| GTTCAGATGGTAGATACCTT+TGG | 0.409315 | 8.4:+14811568 | None:intergenic |
| ACAGTAAAGCCTAAAGATAT+AGG | 0.416419 | 8.4:-14812510 | MS.gene056959:CDS |
| AAAGATCTTATGGCAGTTGC+CGG | 0.419635 | 8.4:-14812054 | MS.gene056959:CDS |
| AGAACATGGCAAATAGCATT+TGG | 0.419756 | 8.4:-14811700 | MS.gene056959:CDS |
| GATACCTTTGGAACATCAAC+AGG | 0.425393 | 8.4:+14811580 | None:intergenic |
| TTGGAACATCAACAGGAAAC+TGG | 0.427742 | 8.4:+14811587 | None:intergenic |
| TCTAATTGTTTGTTTCGAAT+GGG | 0.432398 | 8.4:-14812237 | MS.gene056959:CDS |
| TGGAGAATATCACATTGAAT+TGG | 0.437805 | 8.4:-14812290 | MS.gene056959:CDS |
| TGCGAACCGTGGTGATCAAT+TGG | 0.438062 | 8.4:+14812160 | None:intergenic |
| GCAGCTATCACCATGGCATA+TGG | 0.439140 | 8.4:-14811827 | MS.gene056959:CDS |
| TTGAAGATAGCGACGGAGTT+GGG | 0.440924 | 8.4:+14812457 | None:intergenic |
| AAATTGAAGTATGTTAAACT+TGG | 0.445451 | 8.4:-14813005 | MS.gene056959:CDS |
| CGAAAGATTCTTGAGAGATC+GGG | 0.455522 | 8.4:-14812660 | MS.gene056959:CDS |
| CTGTTCTTCACCACATTGGT+TGG | 0.466086 | 8.4:-14811967 | MS.gene056959:CDS |
| TTCTTGAGAGATCGGGACTC+GGG | 0.466115 | 8.4:-14812653 | MS.gene056959:CDS |
| ATTCTTGAGAGATCGGGACT+CGG | 0.466508 | 8.4:-14812654 | MS.gene056959:CDS |
| GGAAAGTTAAGAACAGCCTC+TGG | 0.468115 | 8.4:+14812615 | None:intergenic |
| TCAAAAGCTAGCTTGAAATC+AGG | 0.468264 | 8.4:+14811907 | None:intergenic |
| GCGAAAGATTCTTGAGAGAT+CGG | 0.468284 | 8.4:-14812661 | MS.gene056959:CDS |
| AACAGTAGTTGTTCTGATGT+TGG | 0.477505 | 8.4:+14811985 | None:intergenic |
| TGTTCTTCACCACATTGGTT+GGG | 0.482146 | 8.4:-14811966 | MS.gene056959:CDS |
| GTGCAGTGTGGAAGGCTCTC+AGG | 0.489224 | 8.4:-14811657 | MS.gene056959:CDS |
| AGCTACAACCTAGGTGGGAT+GGG | 0.489907 | 8.4:-14812396 | MS.gene056959:CDS |
| AAAACTAATATCACTACGCT+AGG | 0.493178 | 8.4:-14812021 | MS.gene056959:CDS |
| TATCACTATCTAATCACTCA+TGG | 0.499740 | 8.4:-14812981 | MS.gene056959:CDS |
| CTGGATGAATTTGGCATCAA+GGG | 0.502606 | 8.4:+14813056 | None:intergenic |
| AGCCAAAGGGAGAATTAAGA+AGG | 0.519483 | 8.4:-14811728 | MS.gene056959:CDS |
| CCTGGATGAATTTGGCATCA+AGG | 0.520908 | 8.4:+14813055 | None:intergenic |
| ATTGAAGATAGCGACGGAGT+TGG | 0.522625 | 8.4:+14812456 | None:intergenic |
| GTCAATCATTACAAGCTTCG+AGG | 0.523795 | 8.4:-14812429 | MS.gene056959:CDS |
| AATATCATAAAAGTCTTGGA+TGG | 0.525987 | 8.4:+14812899 | None:intergenic |
| CTTACTAAATAGCTCATCAA+TGG | 0.525994 | 8.4:+14812536 | None:intergenic |
| CTATGCATTGGTCATTAGCA+TGG | 0.531088 | 8.4:-14812310 | MS.gene056959:CDS |
| GGAAATTTATGGATCAGTCT+AGG | 0.532242 | 8.4:-14812722 | MS.gene056959:CDS |
| CATAGGAATTAGGATTGGCC+TGG | 0.532315 | 8.4:+14812328 | None:intergenic |
| ATCCAAAACAGCTCTACCTC+CGG | 0.535564 | 8.4:+14811867 | None:intergenic |
| CAATCCTAATTCCTATGCAT+TGG | 0.538699 | 8.4:-14812322 | MS.gene056959:CDS |
| AATCGCGTAAGTGCACGAAA+AGG | 0.539987 | 8.4:-14812743 | MS.gene056959:CDS |
| GTGCAACAGTGCAGTGTGGA+AGG | 0.541767 | 8.4:-14811665 | MS.gene056959:CDS |
| GAGCTACAACCTAGGTGGGA+TGG | 0.541792 | 8.4:-14812397 | MS.gene056959:CDS |
| CATGGCATATGGAGCCATCA+AGG | 0.542439 | 8.4:-14811816 | MS.gene056959:CDS |
| AGGTGGGATGGGTTGCAGTG+CGG | 0.542640 | 8.4:-14812385 | MS.gene056959:CDS |
| TCTTTCATTGAAGGATTAGG+AGG | 0.545431 | 8.4:+14812594 | None:intergenic |
| GTGGGATGGGTTGCAGTGCG+GGG | 0.548925 | 8.4:-14812383 | MS.gene056959:CDS |
| GTTTCCTGTTGATGTTCCAA+AGG | 0.557767 | 8.4:-14811584 | MS.gene056959:CDS |
| ACACATCTAGCAGTTCACTT+TGG | 0.560068 | 8.4:-14811771 | MS.gene056959:CDS |
| TGGTCAATCTCATCTATCCA+AGG | 0.561417 | 8.4:+14811607 | None:intergenic |
| GTTTCGAATGGGAGGAGCTG+TGG | 0.564123 | 8.4:-14812226 | MS.gene056959:CDS |
| GAAGCTGAGACTGTGATGTT+CGG | 0.565891 | 8.4:-14812561 | MS.gene056959:CDS |
| TCAATCATTACAAGCTTCGA+GGG | 0.569665 | 8.4:-14812428 | MS.gene056959:CDS |
| ACTACTGTTCTTCACCACAT+TGG | 0.569759 | 8.4:-14811971 | MS.gene056959:CDS |
| AATTGTTTGTTTCGAATGGG+AGG | 0.571309 | 8.4:-14812234 | MS.gene056959:CDS |
| AATATAAAGAGCTACAACCT+AGG | 0.575611 | 8.4:-14812405 | MS.gene056959:CDS |
| CAGGACAATCAACCCTGTGA+AGG | 0.575639 | 8.4:-14811638 | MS.gene056959:CDS |
| TTACAATCTCGTCGTTCAGA+TGG | 0.578695 | 8.4:+14811555 | None:intergenic |
| TCTTGCTAAAGATCTTCTCC+AGG | 0.579124 | 8.4:-14812346 | MS.gene056959:CDS |
| GCAGCAATCAACACCACAAG+TGG | 0.584783 | 8.4:+14812939 | None:intergenic |
| TAAAGAGCTACAACCTAGGT+GGG | 0.586492 | 8.4:-14812401 | MS.gene056959:CDS |
| TGGCTTACACAGAAGCCAAA+GGG | 0.588822 | 8.4:-14811741 | MS.gene056959:CDS |
| GGTAACACCAATATTGCCAT+TGG | 0.592052 | 8.4:+14812083 | None:intergenic |
| AAACAGCTCTACCTCCGGCA+TGG | 0.599316 | 8.4:+14811872 | None:intergenic |
| CCTTGATGCCAAATTCATCC+AGG | 0.603328 | 8.4:-14813055 | MS.gene056959:CDS |
| CAATGCATAGGAATTAGGAT+TGG | 0.604720 | 8.4:+14812323 | None:intergenic |
| CACCACGGTTCGCACTAACA+AGG | 0.615650 | 8.4:-14812151 | MS.gene056959:CDS |
| TCTTGAGAGATCGGGACTCG+GGG | 0.615846 | 8.4:-14812652 | MS.gene056959:CDS |
| GCCAAAGGGAGAATTAAGAA+GGG | 0.617206 | 8.4:-14811727 | MS.gene056959:CDS |
| CATGCTAATGACCAATGCAT+AGG | 0.618448 | 8.4:+14812311 | None:intergenic |
| TATATCTTTAGGCTTTACTG+TGG | 0.620510 | 8.4:+14812512 | None:intergenic |
| TTGGCTTACACAGAAGCCAA+AGG | 0.624548 | 8.4:-14811742 | MS.gene056959:CDS |
| CTGTGAAGGAGAAAAGCCCT+TGG | 0.625413 | 8.4:-14811624 | MS.gene056959:CDS |
| AAACTAATATCACTACGCTA+GGG | 0.626894 | 8.4:-14812020 | MS.gene056959:CDS |
| GGTCAATCTCATCTATCCAA+GGG | 0.627776 | 8.4:+14811608 | None:intergenic |
| CAGCAATCAACACCACAAGT+GGG | 0.638117 | 8.4:+14812940 | None:intergenic |
| CACTGCAACCCATCCCACCT+AGG | 0.640569 | 8.4:+14812388 | None:intergenic |
| ATAAAGAGCTACAACCTAGG+TGG | 0.642158 | 8.4:-14812402 | MS.gene056959:CDS |
| TCTGATGTTGGAAGTACGAG+AGG | 0.642235 | 8.4:+14811997 | None:intergenic |
| GGAATCTACTTATCTTCCAG+AGG | 0.644232 | 8.4:-14812631 | MS.gene056959:CDS |
| TTAAGAAGGGAGATAGAACA+TGG | 0.646961 | 8.4:-14811714 | MS.gene056959:CDS |
| AAAGCGTGGACAGGAAAACA+AGG | 0.654849 | 8.4:+14812826 | None:intergenic |
| AGCAATCAACACCACAAGTG+GGG | 0.662251 | 8.4:+14812941 | None:intergenic |
| ACCCAAGAAGAAGATGCCAA+TGG | 0.668165 | 8.4:-14812099 | MS.gene056959:CDS |
| AAGTTTCTTCCCAACCAATG+TGG | 0.668173 | 8.4:+14811957 | None:intergenic |
| ACAATCATTGAAGATAGCGA+CGG | 0.668618 | 8.4:+14812450 | None:intergenic |
| CTTGATGGCTCCATATGCCA+TGG | 0.676445 | 8.4:+14811817 | None:intergenic |
| GAGAAATCAACAAGGTACAC+CGG | 0.678354 | 8.4:+14812783 | None:intergenic |
| TCAAGTGCAACAGTGCAGTG+TGG | 0.695312 | 8.4:-14811669 | MS.gene056959:CDS |
| AAAACTTGCAGCTATCACCA+TGG | 0.706018 | 8.4:-14811834 | MS.gene056959:CDS |
| AGCCTTGTTAGTGCGAACCG+TGG | 0.715624 | 8.4:+14812149 | None:intergenic |
| AAAATACCAATTGATCACCA+CGG | 0.722409 | 8.4:-14812166 | MS.gene056959:CDS |
| TGTAACATGAGAAATCAACA+AGG | 0.794037 | 8.4:+14812775 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTGAAGTATGTTAAACT+TGG | - | chr8.4:14811645-14811664 | MS.gene056959:CDS | 20.0% |
| ! | AATATCATAAAAGTCTTGGA+TGG | + | chr8.4:14811754-14811773 | None:intergenic | 25.0% |
| ! | ATCAGAATTCCTATATCTTT+AGG | + | chr8.4:14812152-14812171 | None:intergenic | 25.0% |
| ! | TCTAATTGTTTGTTTCGAAT+GGG | - | chr8.4:14812413-14812432 | MS.gene056959:CDS | 25.0% |
| ! | TTAACAGATTTCTTGAAATC+AGG | + | chr8.4:14811627-14811646 | None:intergenic | 25.0% |
| ! | TTCTAATTGTTTGTTTCGAA+TGG | - | chr8.4:14812412-14812431 | MS.gene056959:CDS | 25.0% |
| !! | ATCACATTGAATTGGTATTT+TGG | - | chr8.4:14812368-14812387 | MS.gene056959:CDS | 25.0% |
| !! | CCAAGACTTTTATGATATTT+GGG | - | chr8.4:14811755-14811774 | MS.gene056959:CDS | 25.0% |
| !! | TCCAAGACTTTTATGATATT+TGG | - | chr8.4:14811754-14811773 | MS.gene056959:CDS | 25.0% |
| !!! | ATTAGTTTTTAAAGCATCAC+CGG | + | chr8.4:14812618-14812637 | None:intergenic | 25.0% |
| AAAACTAATATCACTACGCT+AGG | - | chr8.4:14812629-14812648 | MS.gene056959:CDS | 30.0% | |
| AAAATACCAATTGATCACCA+CGG | - | chr8.4:14812484-14812503 | MS.gene056959:CDS | 30.0% | |
| AAACTAATATCACTACGCTA+GGG | - | chr8.4:14812630-14812649 | MS.gene056959:CDS | 30.0% | |
| AATATAAAGAGCTACAACCT+AGG | - | chr8.4:14812245-14812264 | MS.gene056959:CDS | 30.0% | |
| ACAGTAAAGCCTAAAGATAT+AGG | - | chr8.4:14812140-14812159 | MS.gene056959:CDS | 30.0% | |
| AGCTTGAAATCAGGAATATA+AGG | + | chr8.4:14812737-14812756 | None:intergenic | 30.0% | |
| CCCAAATATCATAAAAGTCT+TGG | + | chr8.4:14811758-14811777 | None:intergenic | 30.0% | |
| CTTACTAAATAGCTCATCAA+TGG | + | chr8.4:14812117-14812136 | None:intergenic | 30.0% | |
| TACCTTGTCAAAAGATCTTA+TGG | - | chr8.4:14812586-14812605 | MS.gene056959:CDS | 30.0% | |
| TATATCTTTAGGCTTTACTG+TGG | + | chr8.4:14812141-14812160 | None:intergenic | 30.0% | |
| TATCACTATCTAATCACTCA+TGG | - | chr8.4:14811669-14811688 | MS.gene056959:CDS | 30.0% | |
| TGGAGAATATCACATTGAAT+TGG | - | chr8.4:14812360-14812379 | MS.gene056959:CDS | 30.0% | |
| TGTAACATGAGAAATCAACA+AGG | + | chr8.4:14811878-14811897 | None:intergenic | 30.0% | |
| ! | TAGGATGATCAGTACTTTTA+CGG | - | chr8.4:14811947-14811966 | MS.gene056959:CDS | 30.0% |
| !! | ATTGGTATTTTGGAAACGAT+CGG | - | chr8.4:14812378-14812397 | MS.gene056959:CDS | 30.0% |
| !! | TTTCTAGCTTCTTTCATTGA+AGG | + | chr8.4:14812068-14812087 | None:intergenic | 30.0% |
| AAAGTCTTGGATGGAAAAAG+TGG | + | chr8.4:14811745-14811764 | None:intergenic | 35.0% | |
| AACAGTAGTTGTTCTGATGT+TGG | + | chr8.4:14812668-14812687 | None:intergenic | 35.0% | |
| AATTGTTTGTTTCGAATGGG+AGG | - | chr8.4:14812416-14812435 | MS.gene056959:CDS | 35.0% | |
| ACAATCATTGAAGATAGCGA+CGG | + | chr8.4:14812203-14812222 | None:intergenic | 35.0% | |
| AGAACATGGCAAATAGCATT+TGG | - | chr8.4:14812950-14812969 | MS.gene056959:CDS | 35.0% | |
| ATGACCAATGCATAGGAATT+AGG | + | chr8.4:14812335-14812354 | None:intergenic | 35.0% | |
| CAATCCTAATTCCTATGCAT+TGG | - | chr8.4:14812328-14812347 | MS.gene056959:CDS | 35.0% | |
| CAATGCATAGGAATTAGGAT+TGG | + | chr8.4:14812330-14812349 | None:intergenic | 35.0% | |
| CAGTTCACTTTGGTATGAAT+TGG | - | chr8.4:14812889-14812908 | MS.gene056959:CDS | 35.0% | |
| GCTTCTTTCATTGAAGGATT+AGG | + | chr8.4:14812062-14812081 | None:intergenic | 35.0% | |
| GGAAATTTATGGATCAGTCT+AGG | - | chr8.4:14811928-14811947 | MS.gene056959:CDS | 35.0% | |
| GTGCACGAAAAGGAAATTTA+TGG | - | chr8.4:14811917-14811936 | MS.gene056959:CDS | 35.0% | |
| TCAATCATTACAAGCTTCGA+GGG | - | chr8.4:14812222-14812241 | MS.gene056959:CDS | 35.0% | |
| TCTTTCATTGAAGGATTAGG+AGG | + | chr8.4:14812059-14812078 | None:intergenic | 35.0% | |
| TTAAGAAGGGAGATAGAACA+TGG | - | chr8.4:14812936-14812955 | MS.gene056959:CDS | 35.0% | |
| ! | AGGATGACACTTTATCGTTT+TGG | - | chr8.4:14812854-14812873 | MS.gene056959:CDS | 35.0% |
| ! | GGAGCATCTACAATACAATT+TGG | - | chr8.4:14811776-14811795 | MS.gene056959:CDS | 35.0% |
| ! | TCAAAAGCTAGCTTGAAATC+AGG | + | chr8.4:14812746-14812765 | None:intergenic | 35.0% |
| ! | TGCCATAAGATCTTTTGACA+AGG | + | chr8.4:14812591-14812610 | None:intergenic | 35.0% |
| ! | TTTTGATCTTCTTCTGTCTG+AGG | + | chr8.4:14812468-14812487 | None:intergenic | 35.0% |
| !! | TCTTCTGTCTGAGGTTTTAT+TGG | + | chr8.4:14812459-14812478 | None:intergenic | 35.0% |
| AAAACTTGCAGCTATCACCA+TGG | - | chr8.4:14812816-14812835 | MS.gene056959:CDS | 40.0% | |
| AAAGATCTTATGGCAGTTGC+CGG | - | chr8.4:14812596-14812615 | MS.gene056959:CDS | 40.0% | |
| AAGTTTCTTCCCAACCAATG+TGG | + | chr8.4:14812696-14812715 | None:intergenic | 40.0% | |
| ACACATCTAGCAGTTCACTT+TGG | - | chr8.4:14812879-14812898 | MS.gene056959:CDS | 40.0% | |
| ACGAGTCAAAAAGTAAAGCG+TGG | + | chr8.4:14811841-14811860 | None:intergenic | 40.0% | |
| ACTACTGTTCTTCACCACAT+TGG | - | chr8.4:14812679-14812698 | MS.gene056959:CDS | 40.0% | |
| AGCCAAAGGGAGAATTAAGA+AGG | - | chr8.4:14812922-14812941 | MS.gene056959:CDS | 40.0% | |
| ATAAAGAGCTACAACCTAGG+TGG | - | chr8.4:14812248-14812267 | MS.gene056959:CDS | 40.0% | |
| CATGCTAATGACCAATGCAT+AGG | + | chr8.4:14812342-14812361 | None:intergenic | 40.0% | |
| CGAAAGATTCTTGAGAGATC+GGG | - | chr8.4:14811990-14812009 | MS.gene056959:CDS | 40.0% | |
| CTGGATGAATTTGGCATCAA+GGG | + | chr8.4:14811597-14811616 | None:intergenic | 40.0% | |
| GAAGATGCCAATGGCAATAT+TGG | - | chr8.4:14812560-14812579 | MS.gene056959:CDS | 40.0% | |
| GAGAAATCAACAAGGTACAC+CGG | + | chr8.4:14811870-14811889 | None:intergenic | 40.0% | |
| GCCAAAGGGAGAATTAAGAA+GGG | - | chr8.4:14812923-14812942 | MS.gene056959:CDS | 40.0% | |
| GCGAAAGATTCTTGAGAGAT+CGG | - | chr8.4:14811989-14812008 | MS.gene056959:CDS | 40.0% | |
| GGAATCTACTTATCTTCCAG+AGG | - | chr8.4:14812019-14812038 | MS.gene056959:CDS | 40.0% | |
| GGTAACACCAATATTGCCAT+TGG | + | chr8.4:14812570-14812589 | None:intergenic | 40.0% | |
| GGTCAATCTCATCTATCCAA+GGG | + | chr8.4:14813045-14813064 | None:intergenic | 40.0% | |
| GTCAATCATTACAAGCTTCG+AGG | - | chr8.4:14812221-14812240 | MS.gene056959:CDS | 40.0% | |
| GTTTCCTGTTGATGTTCCAA+AGG | - | chr8.4:14813066-14813085 | MS.gene056959:CDS | 40.0% | |
| TAAAGAGCTACAACCTAGGT+GGG | - | chr8.4:14812249-14812268 | MS.gene056959:CDS | 40.0% | |
| TCAAAAAGTAAAGCGTGGAC+AGG | + | chr8.4:14811836-14811855 | None:intergenic | 40.0% | |
| TCCCTTCTTAATTCTCCCTT+TGG | + | chr8.4:14812927-14812946 | None:intergenic | 40.0% | |
| TCTTGCTAAAGATCTTCTCC+AGG | - | chr8.4:14812304-14812323 | MS.gene056959:CDS | 40.0% | |
| TGGTCAATCTCATCTATCCA+AGG | + | chr8.4:14813046-14813065 | None:intergenic | 40.0% | |
| TTGGAACATCAACAGGAAAC+TGG | + | chr8.4:14813066-14813085 | None:intergenic | 40.0% | |
| ! | CGATAAAGTGTCATCCTTGA+TGG | + | chr8.4:14812851-14812870 | None:intergenic | 40.0% |
| ! | CTATGCATTGGTCATTAGCA+TGG | - | chr8.4:14812340-14812359 | MS.gene056959:CDS | 40.0% |
| ! | GAACATTTTTGCATCCATGC+CGG | - | chr8.4:14812764-14812783 | MS.gene056959:CDS | 40.0% |
| ! | GATGATCAGTACTTTTACGG+AGG | - | chr8.4:14811950-14811969 | MS.gene056959:CDS | 40.0% |
| ! | TGGCAAATAGCATTTGGTTC+TGG | - | chr8.4:14812956-14812975 | MS.gene056959:CDS | 40.0% |
| ! | TGTTCTTCACCACATTGGTT+GGG | - | chr8.4:14812684-14812703 | MS.gene056959:CDS | 40.0% |
| !! | GATACCTTTGGAACATCAAC+AGG | + | chr8.4:14813073-14813092 | None:intergenic | 40.0% |
| !! | TAGAGCTGTTTTGGATGAAC+TGG | - | chr8.4:14812790-14812809 | MS.gene056959:CDS | 40.0% |
| AAAGCGTGGACAGGAAAACA+AGG | + | chr8.4:14811827-14811846 | None:intergenic | 45.0% | |
| AATCGCGTAAGTGCACGAAA+AGG | - | chr8.4:14811907-14811926 | MS.gene056959:CDS | 45.0% | |
| ACCCAAGAAGAAGATGCCAA+TGG | - | chr8.4:14812551-14812570 | MS.gene056959:CDS | 45.0% | |
| AGCAATCAACACCACAAGTG+GGG | + | chr8.4:14811712-14811731 | None:intergenic | 45.0% | |
| ATCCAAAACAGCTCTACCTC+CGG | + | chr8.4:14812786-14812805 | None:intergenic | 45.0% | |
| ATTCTTGAGAGATCGGGACT+CGG | - | chr8.4:14811996-14812015 | MS.gene056959:CDS | 45.0% | |
| ATTGAAGATAGCGACGGAGT+TGG | + | chr8.4:14812197-14812216 | None:intergenic | 45.0% | |
| CAGCAATCAACACCACAAGT+GGG | + | chr8.4:14811713-14811732 | None:intergenic | 45.0% | |
| CCACAAGTGGGGAAAGAAAA+AGG | + | chr8.4:14811701-14811720 | None:intergenic | 45.0% | |
| CCTGGATGAATTTGGCATCA+AGG | + | chr8.4:14811598-14811617 | None:intergenic | 45.0% | |
| CCTTGATGCCAAATTCATCC+AGG | - | chr8.4:14811595-14811614 | MS.gene056959:CDS | 45.0% | |
| CTGTTCTTCACCACATTGGT+TGG | - | chr8.4:14812683-14812702 | MS.gene056959:CDS | 45.0% | |
| CTTGAAATCAGGCAAGTTCC+TGG | + | chr8.4:14811616-14811635 | None:intergenic | 45.0% | |
| GAAGCTGAGACTGTGATGTT+CGG | - | chr8.4:14812089-14812108 | MS.gene056959:CDS | 45.0% | |
| GCCATTGGCATCTTCTTCTT+GGG | + | chr8.4:14812555-14812574 | None:intergenic | 45.0% | |
| GGAAAGTTAAGAACAGCCTC+TGG | + | chr8.4:14812038-14812057 | None:intergenic | 45.0% | |
| GGCAAGTTCCTGGATGAATT+TGG | + | chr8.4:14811606-14811625 | None:intergenic | 45.0% | |
| TGCCATTGGCATCTTCTTCT+TGG | + | chr8.4:14812556-14812575 | None:intergenic | 45.0% | |
| TGGCTTACACAGAAGCCAAA+GGG | - | chr8.4:14812909-14812928 | MS.gene056959:CDS | 45.0% | |
| TTGAAGATAGCGACGGAGTT+GGG | + | chr8.4:14812196-14812215 | None:intergenic | 45.0% | |
| TTGGCTTACACAGAAGCCAA+AGG | - | chr8.4:14812908-14812927 | MS.gene056959:CDS | 45.0% | |
| ! | AAGGGCTTTTCTCCTTCACA+GGG | + | chr8.4:14813027-14813046 | None:intergenic | 45.0% |
| ! | CCTTTTTCTTTCCCCACTTG+TGG | - | chr8.4:14811698-14811717 | MS.gene056959:CDS | 45.0% |
| ! | TCTGATGTTGGAAGTACGAG+AGG | + | chr8.4:14812656-14812675 | None:intergenic | 45.0% |
| !! | CATAGGAATTAGGATTGGCC+TGG | + | chr8.4:14812325-14812344 | None:intergenic | 45.0% |
| !! | CTTTTTGACTCGTCCGAAAC+CGG | - | chr8.4:14811848-14811867 | MS.gene056959:CDS | 45.0% |
| !! | TCAACAAGGTACACCGGTTT+CGG | + | chr8.4:14811864-14811883 | None:intergenic | 45.0% |
| AGCTACAACCTAGGTGGGAT+GGG | - | chr8.4:14812254-14812273 | MS.gene056959:CDS | 50.0% | |
| CAGGACAATCAACCCTGTGA+AGG | - | chr8.4:14813012-14813031 | MS.gene056959:CDS | 50.0% | |
| CATGGCATATGGAGCCATCA+AGG | - | chr8.4:14812834-14812853 | MS.gene056959:CDS | 50.0% | |
| CTGTGAAGGAGAAAAGCCCT+TGG | - | chr8.4:14813026-14813045 | MS.gene056959:CDS | 50.0% | |
| GCAGCAATCAACACCACAAG+TGG | + | chr8.4:14811714-14811733 | None:intergenic | 50.0% | |
| GCAGCTATCACCATGGCATA+TGG | - | chr8.4:14812823-14812842 | MS.gene056959:CDS | 50.0% | |
| GTGCACTTACGCGATTCTTC+AGG | + | chr8.4:14811903-14811922 | None:intergenic | 50.0% | |
| TCAAGTGCAACAGTGCAGTG+TGG | - | chr8.4:14812981-14813000 | MS.gene056959:CDS | 50.0% | |
| TGCGAACCGTGGTGATCAAT+TGG | + | chr8.4:14812493-14812512 | None:intergenic | 50.0% | |
| TTCTTGAGAGATCGGGACTC+GGG | - | chr8.4:14811997-14812016 | MS.gene056959:CDS | 50.0% | |
| ! | CAAGGGCTTTTCTCCTTCAC+AGG | + | chr8.4:14813028-14813047 | None:intergenic | 50.0% |
| ! | CATTTTTGCATCCATGCCGG+AGG | - | chr8.4:14812767-14812786 | MS.gene056959:CDS | 50.0% |
| ! | CTTGATGGCTCCATATGCCA+TGG | + | chr8.4:14812836-14812855 | None:intergenic | 50.0% |
| AAACAGCTCTACCTCCGGCA+TGG | + | chr8.4:14812781-14812800 | None:intergenic | 55.0% | |
| CACCACGGTTCGCACTAACA+AGG | - | chr8.4:14812499-14812518 | MS.gene056959:CDS | 55.0% | |
| GAGCTACAACCTAGGTGGGA+TGG | - | chr8.4:14812253-14812272 | MS.gene056959:CDS | 55.0% | |
| GTGCAACAGTGCAGTGTGGA+AGG | - | chr8.4:14812985-14813004 | MS.gene056959:CDS | 55.0% | |
| GTTTCGAATGGGAGGAGCTG+TGG | - | chr8.4:14812424-14812443 | MS.gene056959:CDS | 55.0% | |
| TCTTGAGAGATCGGGACTCG+GGG | - | chr8.4:14811998-14812017 | MS.gene056959:CDS | 55.0% | |
| ! | TGCCGGAGGTAGAGCTGTTT+TGG | - | chr8.4:14812781-14812800 | MS.gene056959:CDS | 55.0% |
| !! | AGCCTTGTTAGTGCGAACCG+TGG | + | chr8.4:14812504-14812523 | None:intergenic | 55.0% |
| CACTGCAACCCATCCCACCT+AGG | + | chr8.4:14812265-14812284 | None:intergenic | 60.0% | |
| GTGCAGTGTGGAAGGCTCTC+AGG | - | chr8.4:14812993-14813012 | MS.gene056959:CDS | 60.0% | |
| !! | AGGTGGGATGGGTTGCAGTG+CGG | - | chr8.4:14812265-14812284 | MS.gene056959:CDS | 60.0% |
| !! | GGTGGGATGGGTTGCAGTGC+GGG | - | chr8.4:14812266-14812285 | MS.gene056959:CDS | 65.0% |
| !! | GTGGGATGGGTTGCAGTGCG+GGG | - | chr8.4:14812267-14812286 | MS.gene056959:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 14811570 | 14813102 | 14811570 | ID=MS.gene056959 |
| chr8.4 | mRNA | 14811570 | 14813102 | 14811570 | ID=MS.gene056959.t1;Parent=MS.gene056959 |
| chr8.4 | exon | 14811570 | 14813102 | 14811570 | ID=MS.gene056959.t1.exon1;Parent=MS.gene056959.t1 |
| chr8.4 | CDS | 14811570 | 14813102 | 14811570 | ID=cds.MS.gene056959.t1;Parent=MS.gene056959.t1 |
| Gene Sequence |
| Protein sequence |
